##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931088_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1974103 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.133037131294568 33.0 33.0 33.0 27.0 33.0 2 31.06007791893331 33.0 33.0 33.0 27.0 33.0 3 31.07821425731079 33.0 33.0 33.0 27.0 33.0 4 34.70132612128141 37.0 37.0 37.0 33.0 37.0 5 34.68459396495522 37.0 37.0 37.0 33.0 37.0 6 34.77297841095424 37.0 37.0 37.0 33.0 37.0 7 34.74133517855958 37.0 37.0 37.0 33.0 37.0 8 34.7391174624627 37.0 37.0 37.0 33.0 37.0 9 34.751209030126596 37.0 37.0 37.0 33.0 37.0 10-11 34.6602578487546 37.0 37.0 37.0 33.0 37.0 12-13 34.57088789186785 37.0 37.0 37.0 33.0 37.0 14-15 36.24816055697195 40.0 37.0 40.0 33.0 40.0 16-17 36.205787894552614 40.0 37.0 40.0 33.0 40.0 18-19 36.14257893331807 40.0 37.0 40.0 33.0 40.0 20-21 36.0569790431401 40.0 37.0 40.0 33.0 40.0 22-23 35.97484477760279 40.0 37.0 40.0 33.0 40.0 24-25 35.85790736349623 40.0 37.0 40.0 27.0 40.0 26-27 35.686649582114 40.0 37.0 40.0 27.0 40.0 28-29 35.57905818490727 40.0 37.0 40.0 27.0 40.0 30-31 35.447267695758526 40.0 37.0 40.0 27.0 40.0 32-33 35.29584778504465 38.5 37.0 40.0 27.0 40.0 34-35 35.183120890855236 37.0 37.0 40.0 27.0 40.0 36-37 34.983121954629524 37.0 37.0 40.0 27.0 40.0 38-39 34.81589638433253 37.0 37.0 40.0 27.0 40.0 40-41 34.60262179835601 37.0 37.0 40.0 22.0 40.0 42-43 34.43599802036672 37.0 37.0 40.0 22.0 40.0 44-45 34.18711536328145 37.0 33.0 40.0 22.0 40.0 46-47 34.04471017976266 37.0 33.0 40.0 22.0 40.0 48-49 34.04454985378169 37.0 33.0 40.0 18.5 40.0 50-51 33.24211907889304 37.0 33.0 38.5 15.0 40.0 52-53 33.36114275698887 37.0 33.0 38.5 18.5 40.0 54-55 33.87632889469293 37.0 33.0 40.0 22.0 40.0 56-57 33.837182507700966 37.0 33.0 40.0 18.5 40.0 58-59 33.70409624016578 37.0 33.0 40.0 15.0 40.0 60-61 33.72001080997293 37.0 33.0 40.0 15.0 40.0 62-63 33.56134026441376 37.0 33.0 40.0 15.0 40.0 64-65 33.354146921411896 37.0 33.0 40.0 15.0 40.0 66-67 33.13587335615213 37.0 33.0 40.0 15.0 40.0 68-69 32.9314184720858 37.0 33.0 40.0 10.5 40.0 70-71 32.698828531236714 37.0 33.0 40.0 6.0 40.0 72-73 32.09792194226948 37.0 33.0 38.5 2.0 40.0 74-75 31.917231775646965 37.0 33.0 37.0 2.0 40.0 76-77 31.560676671885915 37.0 33.0 37.0 2.0 40.0 78-79 31.29445044154231 37.0 33.0 37.0 2.0 38.5 80-81 31.020904684304718 37.0 33.0 37.0 2.0 37.0 82-83 30.76038458986183 37.0 33.0 37.0 2.0 37.0 84-85 30.38783462666335 37.0 33.0 37.0 2.0 37.0 86-87 30.225681993290117 37.0 33.0 37.0 2.0 37.0 88-89 30.026536862564924 37.0 33.0 37.0 2.0 37.0 90-91 29.758626576222213 33.0 33.0 37.0 2.0 37.0 92-93 29.48989110497274 33.0 30.0 37.0 2.0 37.0 94-95 29.25743844166186 33.0 27.0 37.0 2.0 37.0 96-97 28.972493329881978 33.0 27.0 37.0 2.0 37.0 98-99 28.674078049625578 33.0 27.0 37.0 2.0 37.0 100-101 27.991319601864745 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.03052900524354385 1101 2 -0.012090643849180793 1101 3 0.00264105602003184 1101 4 0.03500115705824669 1101 5 -0.15131725181789335 1101 6 0.013040500269895006 1101 7 -0.03280166281289354 1101 8 -0.0033207341679073465 1101 9 0.058111314665509894 1101 10-11 7.761935556729327E-4 1101 12-13 0.08432145670052194 1101 14-15 0.1014186351942925 1101 16-17 0.12722485865892708 1101 18-19 0.11433994329478736 1101 20-21 -0.004655994344169301 1101 22-23 0.09106256962050452 1101 24-25 0.08439373224550195 1101 26-27 0.1294249059227539 1101 28-29 0.16999293700527573 1101 30-31 0.16695645130935333 1101 32-33 -0.013014435146558867 1101 34-35 0.11593311329887968 1101 36-37 0.1477180480901623 1101 38-39 0.16579324664883188 1101 40-41 0.18686399929358544 1101 42-43 0.02330426018927767 1101 44-45 0.10109876847655386 1101 46-47 0.02292811820002072 1101 48-49 -0.08402554307385657 1101 50-51 -0.015418771365979467 1101 52-53 0.08718640196046579 1101 54-55 0.2534360917493004 1101 56-57 0.33844996406233463 1101 58-59 0.38855020622301595 1101 60-61 0.34097347524382826 1101 62-63 0.3258575013095708 1101 64-65 0.3793269198655267 1101 66-67 0.3534925435779357 1101 68-69 0.2670918822939825 1101 70-71 0.1840120874310074 1101 72-73 0.09939973924868895 1101 74-75 0.022740261177670362 1101 76-77 -0.001744187720355228 1101 78-79 -0.03565565890122713 1101 80-81 0.0972654303579148 1101 82-83 0.11079144786572925 1101 84-85 0.15546168405388983 1101 86-87 -0.09700385674623746 1101 88-89 -0.01541056516096262 1101 90-91 -0.12620664376056467 1101 92-93 -0.08847446583834895 1101 94-95 -0.1648792330510176 1101 96-97 -0.2158475588493438 1101 98-99 -0.1145682923884479 1101 100-101 -0.0376362752386008 1102 1 0.05653765638063746 1102 2 0.0660873810102629 1102 3 0.03727850149818579 1102 4 0.10076175257547959 1102 5 0.028746661653094918 1102 6 0.10823785044512135 1102 7 0.06991434454913303 1102 8 -0.03617849833097608 1102 9 0.16460823875092956 1102 10-11 0.08595539079239245 1102 12-13 0.04501199322070448 1102 14-15 0.16329573097625172 1102 16-17 0.2050062607212979 1102 18-19 0.18233435874495285 1102 20-21 0.31778321222114414 1102 22-23 0.31459111007382745 1102 24-25 0.34251291763238356 1102 26-27 0.16922538117676567 1102 28-29 0.36842645817110053 1102 30-31 0.22070742829042445 1102 32-33 0.2486420520077104 1102 34-35 0.2859777707882287 1102 36-37 0.42885979228500304 1102 38-39 0.4221399992898327 1102 40-41 0.3442523398124635 1102 42-43 0.40142792077975287 1102 44-45 0.3242629895390081 1102 46-47 0.4125445428275114 1102 48-49 0.450601502283277 1102 50-51 0.4273287199987621 1102 52-53 0.5029216274855131 1102 54-55 0.465877788040558 1102 56-57 0.669484713072741 1102 58-59 0.6746679355929572 1102 60-61 0.56345617322431 1102 62-63 0.6707831877175536 1102 64-65 0.6963134690423374 1102 66-67 0.8402637472186072 1102 68-69 0.7969846665009257 1102 70-71 0.6954525357077372 1102 72-73 0.6919187431061928 1102 74-75 0.6816462314774547 1102 76-77 0.6736314772570786 1102 78-79 0.7581546933162002 1102 80-81 0.7436778613389308 1102 82-83 0.7822755766507434 1102 84-85 0.8071775464445317 1102 86-87 0.7220918684888638 1102 88-89 0.683131054232426 1102 90-91 0.5988050169811849 1102 92-93 0.5148664942503984 1102 94-95 0.5936318478051952 1102 96-97 0.576375829447052 1102 98-99 0.5931635672792517 1102 100-101 0.6164106324587486 1103 1 0.04764837695995183 1103 2 0.012543465442167445 1103 3 0.09327313783818525 1103 4 -8.017254258163575E-4 1103 5 -0.00828738843982535 1103 6 -0.04145171689914662 1103 7 0.045800744734023624 1103 8 0.08031439506026317 1103 9 0.02349980519001349 1103 10-11 -0.016173823339933335 1103 12-13 0.057513858946244056 1103 14-15 0.13743527039482473 1103 16-17 0.16606104564527158 1103 18-19 0.15342584429581052 1103 20-21 0.1990469343315553 1103 22-23 0.15042351255054598 1103 24-25 0.20847648663293228 1103 26-27 0.09742353567328621 1103 28-29 0.10012338971461787 1103 30-31 0.01308828666861217 1103 32-33 0.1043477416280183 1103 34-35 0.10653078387478843 1103 36-37 0.10125403573931635 1103 38-39 0.21890079883146285 1103 40-41 0.005127533607172552 1103 42-43 -0.07120218649345134 1103 44-45 0.09736127355299118 1103 46-47 0.07790891300671632 1103 48-49 -0.1271890212754201 1103 50-51 -0.19683561551405404 1103 52-53 -0.017537858585342292 1103 54-55 0.15576892596461533 1103 56-57 0.1873207482976369 1103 58-59 0.08776665293674313 1103 60-61 0.051914855227643386 1103 62-63 0.10572413449502704 1103 64-65 0.06495495603763146 1103 66-67 0.06703817257514544 1103 68-69 0.06602048361649082 1103 70-71 0.14988249035255308 1103 72-73 9.259243439743159E-4 1103 74-75 0.08529354435119885 1103 76-77 0.18786997235082126 1103 78-79 0.03790519164547845 1103 80-81 -0.01674041025293249 1103 82-83 0.05959340961655002 1103 84-85 -0.02956214030616877 1103 86-87 -0.1923312130638024 1103 88-89 -0.2259506356575045 1103 90-91 -0.1431585207233219 1103 92-93 -0.1939340729301655 1103 94-95 -0.012070531656895866 1103 96-97 0.05986445935451101 1103 98-99 0.08511115603964114 1103 100-101 -0.019821637130167602 1104 1 -0.0018227737189420168 1104 2 0.0483998128834493 1104 3 -0.1436686228574935 1104 4 0.09198032186621674 1104 5 -0.03202938357387097 1104 6 -0.09200532763605906 1104 7 0.041649800051935415 1104 8 -0.03241535793893746 1104 9 -0.007148694018297874 1104 10-11 -0.1288507595577073 1104 12-13 -0.021155885102494665 1104 14-15 -0.011398160242542588 1104 16-17 -0.02640955301306036 1104 18-19 -0.025171830402790363 1104 20-21 0.01217037112520103 1104 22-23 0.07108163236399179 1104 24-25 0.08233950602634366 1104 26-27 0.10001950887643574 1104 28-29 0.05761865892119289 1104 30-31 -0.10276560734798323 1104 32-33 0.0752252498572119 1104 34-35 0.03119446024081185 1104 36-37 0.16106137375081886 1104 38-39 0.2314859587924829 1104 40-41 0.18662132848724156 1104 42-43 0.11724269436778911 1104 44-45 0.11561827433217786 1104 46-47 -0.13803846103377992 1104 48-49 -0.1543172536056474 1104 50-51 -0.04572481612922985 1104 52-53 0.08273565283330697 1104 54-55 0.13804463637873 1104 56-57 0.17147713367613449 1104 58-59 0.17435039536349706 1104 60-61 0.23241932932015885 1104 62-63 0.14239970997078188 1104 64-65 0.14956346442357216 1104 66-67 0.2348467418423681 1104 68-69 0.14346493512840652 1104 70-71 0.19063631644562662 1104 72-73 0.10634396065011487 1104 74-75 0.16439860090490654 1104 76-77 0.05385761208812667 1104 78-79 0.17597990069884162 1104 80-81 0.3082730495074948 1104 82-83 0.22108384239611922 1104 84-85 0.3547040812607989 1104 86-87 0.3191927839284787 1104 88-89 0.361830571109401 1104 90-91 0.43125578092524464 1104 92-93 0.1949857046259993 1104 94-95 0.2559953632411123 1104 96-97 0.29169293089364956 1104 98-99 0.3434343424931008 1104 100-101 0.39870620583247884 1105 1 -0.07013798613692757 1105 2 -0.07644935683174481 1105 3 -0.064124933681736 1105 4 -0.11313130850541597 1105 5 -0.07231623244990004 1105 6 -0.03868589090603791 1105 7 -0.1711560369470817 1105 8 -0.09038260196371795 1105 9 -0.02426407413189935 1105 10-11 -0.04740985417165433 1105 12-13 -0.14189459703846552 1105 14-15 -0.12618532727206144 1105 16-17 -0.12614428324232563 1105 18-19 -0.007091615292736719 1105 20-21 0.1073764686057217 1105 22-23 0.009290960325962772 1105 24-25 -6.450821511094773E-4 1105 26-27 -0.058835372704500344 1105 28-29 -0.1709333809852609 1105 30-31 -0.13703999397149147 1105 32-33 -0.18710027115901084 1105 34-35 -0.08235095058151387 1105 36-37 -0.05560273917558334 1105 38-39 -0.1863761775389392 1105 40-41 -0.0012956494165194954 1105 42-43 0.0668730936663664 1105 44-45 0.041456592779333334 1105 46-47 0.10151354907902999 1105 48-49 0.12719403924663908 1105 50-51 0.13150874895602982 1105 52-53 0.08465627832942602 1105 54-55 0.17153640801475944 1105 56-57 0.09643893397790748 1105 58-59 0.15153538618067586 1105 60-61 0.15829488981295015 1105 62-63 0.17101942399443004 1105 64-65 0.17333878635445643 1105 66-67 0.11292692503067059 1105 68-69 0.08434664587902319 1105 70-71 0.10928718610059462 1105 72-73 0.09628674622744171 1105 74-75 0.1252782261009564 1105 76-77 0.22701636535212444 1105 78-79 0.20436498983501394 1105 80-81 0.2612464544306832 1105 82-83 0.09203376993374235 1105 84-85 0.17386389007077696 1105 86-87 0.19048666835896455 1105 88-89 0.1581838832041278 1105 90-91 0.11089704426055391 1105 92-93 0.3055831415220105 1105 94-95 0.3138277378577854 1105 96-97 0.22262835531464376 1105 98-99 0.20313211797251185 1105 100-101 0.4662438575406149 1106 1 0.03697788594066509 1106 2 -0.05327328597472203 1106 3 -0.0202082751582644 1106 4 0.05724785473710625 1106 5 -0.05993723901305259 1106 6 0.0441541783191397 1106 7 0.11169188178541845 1106 8 0.05513471527758895 1106 9 0.06273836989497283 1106 10-11 0.07120834953000355 1106 12-13 0.028918425027192995 1106 14-15 0.17505259877063395 1106 16-17 0.05297706853895079 1106 18-19 -0.01226174401931246 1106 20-21 0.24067538692226265 1106 22-23 0.1491686468465403 1106 24-25 0.15640753998481927 1106 26-27 0.25209335750687245 1106 28-29 0.1167371789302436 1106 30-31 0.10586541305750785 1106 32-33 0.20430806928960976 1106 34-35 0.1442337554811317 1106 36-37 0.11983975677551939 1106 38-39 0.16294812405515557 1106 40-41 0.27239184087171964 1106 42-43 0.26418140682859814 1106 44-45 0.36904069597846245 1106 46-47 0.30855311540175734 1106 48-49 0.3407031748450109 1106 50-51 0.43391822731621943 1106 52-53 0.5224334473698988 1106 54-55 0.43060646032878935 1106 56-57 0.47918518290126855 1106 58-59 0.3653318030674697 1106 60-61 0.33093465776656217 1106 62-63 0.5362200216249491 1106 64-65 0.4726949665599207 1106 66-67 0.2966187458974332 1106 68-69 0.29244950005599435 1106 70-71 0.326864582509792 1106 72-73 0.37552228965096646 1106 74-75 0.27149746017059684 1106 76-77 0.23489751099572587 1106 78-79 0.22506272559924057 1106 80-81 0.23504284995827618 1106 82-83 0.11611053170759789 1106 84-85 0.32193777048806993 1106 86-87 0.3378475518610635 1106 88-89 0.3327450450166509 1106 90-91 0.3899674543412459 1106 92-93 0.4546374093845813 1106 94-95 0.48642251798881375 1106 96-97 0.5552105121563429 1106 98-99 0.5529272565109764 1106 100-101 0.4448655641516588 1107 1 0.026018124479033844 1107 2 0.07942747947174666 1107 3 0.08681346131375278 1107 4 0.07240943197255945 1107 5 -0.021517563294182196 1107 6 4.437491521187553E-4 1107 7 -0.016741493635272775 1107 8 -0.05280911164081914 1107 9 -0.056130532490790586 1107 10-11 -0.025913826947778773 1107 12-13 0.0426738269910274 1107 14-15 0.10228834529551278 1107 16-17 0.14282290828307254 1107 18-19 0.12754243455431435 1107 20-21 0.0766211410654023 1107 22-23 0.12501238365731382 1107 24-25 0.16777973564116877 1107 26-27 0.2160332155373439 1107 28-29 0.0652717716267901 1107 30-31 0.08249831953367703 1107 32-33 0.16763547899779496 1107 34-35 0.14638618599458653 1107 36-37 0.2042766068720212 1107 38-39 0.1495847750996191 1107 40-41 0.27899901641652747 1107 42-43 0.24771163592579626 1107 44-45 0.049205193673365955 1107 46-47 0.0818657114053778 1107 48-49 0.14585662745597006 1107 50-51 0.08244606004162591 1107 52-53 0.1651693520072186 1107 54-55 0.31472903506254823 1107 56-57 0.281644647759677 1107 58-59 0.4387792693568642 1107 60-61 0.3262147041293275 1107 62-63 0.27028376941877497 1107 64-65 0.41136835640188707 1107 66-67 0.4113169739494893 1107 68-69 0.3817812528557525 1107 70-71 0.346567872944739 1107 72-73 0.43032388196596827 1107 74-75 0.42017340462338026 1107 76-77 0.4559089444615374 1107 78-79 0.47257147368549823 1107 80-81 0.6248067092283378 1107 82-83 0.5070597911616233 1107 84-85 0.5649012608206796 1107 86-87 0.514758022246184 1107 88-89 0.7194714745543287 1107 90-91 0.6643477206942343 1107 92-93 0.6490151894860077 1107 94-95 0.5607684021664561 1107 96-97 0.6327186450092732 1107 98-99 0.4904990495566146 1107 100-101 0.4875630103763413 1108 1 -0.054267060560547975 1108 2 -0.028404933847678393 1108 3 0.026096937370652995 1108 4 0.11754045012385461 1108 5 -0.05232735282799439 1108 6 0.006316035650691276 1108 7 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0.009057455746031451 2316 74-75 -0.15127871425639583 2316 76-77 -0.28138188056559343 2316 78-79 -0.2253421342009112 2316 80-81 -0.20884447127085792 2316 82-83 -0.17471223433606298 2316 84-85 -0.15463682156348213 2316 86-87 -0.207851569480745 2316 88-89 -0.3047832469864744 2316 90-91 -0.25929942449485566 2316 92-93 -0.16436209149012626 2316 94-95 -0.1627465125220695 2316 96-97 0.0030653852994468878 2316 98-99 -0.05599843806387739 2316 100-101 -0.2604323296384301 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58893.0 3 8624.0 4 6631.0 5 5494.0 6 5662.0 7 6004.0 8 5929.0 9 6130.0 10 6284.0 11 6240.0 12 6537.0 13 7115.0 14 7310.0 15 8242.0 16 9504.0 17 11805.0 18 10499.0 19 8345.0 20 8630.0 21 9030.0 22 9932.0 23 11247.0 24 12891.0 25 14987.0 26 16983.0 27 19436.0 28 23867.0 29 28462.0 30 35103.0 31 44483.0 32 56924.0 33 75691.0 34 103852.0 35 151174.0 36 236218.0 37 435113.0 38 494557.0 39 275.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.459349727343415 23.431597770117445 13.20493694579035 22.90411555674879 2 14.113413885404782 21.92321954323207 43.3525797803899 20.61078679097325 3 17.297502659439743 31.08935717542171 29.992502912719722 21.620637252418824 4 10.96611537216558 18.1479382051699 42.915108346618 27.970838076046523 5 11.68438217267768 38.57156100261845 36.56514887255828 13.178907952145591 6 25.374157275481572 41.434261535492325 20.805651984724204 12.385929204301902 7 23.913240595855434 32.76054998143461 27.24893280644424 16.077276616265717 8 22.97473840017466 37.513949373462275 21.946018014257614 17.56529421210545 9 25.63984756621108 15.92110442059001 23.890445432685123 34.54860258051378 10-11 22.877825684241667 27.598783547857085 30.644878188887454 18.878512579013794 12-13 23.31670298697838 25.606554271258524 30.16933775458411 20.90740498717898 14-15 21.021423847609864 27.10276420647318 27.635605891171828 24.240206054745133 16-17 19.421218297692235 29.36119575979536 29.44369287163657 21.773893070875836 18-19 19.151570905603823 30.207293532819683 30.70824678267094 19.932888778905557 20-21 20.07091321982693 28.62781222661634 29.884078996891244 21.417195556665483 22-23 20.25337583702573 29.453680988276698 29.219448022722215 21.07349515197535 24-25 20.28194577486585 29.145920957518427 29.821848201436296 20.750285066179426 26-27 20.38556245545445 28.798142751416723 29.41069944172113 21.4055953514077 28-29 19.892680371794178 30.090121943991775 28.921895159472427 21.095302524741616 30-31 21.1977794471717 29.2726114088272 28.75564243608363 20.773966707917467 32-33 21.121972865650882 29.461203392122904 28.699211743257568 20.717611998968646 34-35 21.418943185841872 28.327650583581505 29.511530046811135 20.741876183765488 36-37 21.415143991980155 28.95659446340946 29.404620731542373 20.223640813068013 38-39 21.861827878281932 28.48519048904743 30.059095194121078 19.59388643854956 40-41 20.855530185312528 28.587721731069514 30.094638182764527 20.462109900853427 42-43 21.41965779385822 28.505536060057672 29.1694326915649 20.905373454519204 44-45 20.826611897252132 29.588629115779412 29.126898433686936 20.457860553281517 46-47 21.962870691496434 28.720209881057702 28.309419920582652 21.007499506863216 48-49 21.792760498326576 27.777915004979647 29.357881101089568 21.07144339560421 50-51 21.531358336039812 28.421705319104834 28.412498623446403 21.63443772140895 52-53 20.576052824539623 28.875839203362023 28.883616488352498 21.664491483745856 54-55 20.978338546489937 29.233228866893217 28.40228355573537 21.38614903088147 56-57 21.58516512898888 28.162252972108405 28.89625838372481 21.356323515177905 58-59 21.32666854829306 27.79445018444935 29.80790897873669 21.070972288520903 60-61 21.165778486899285 29.553781735854272 28.44115688399006 20.839282893256385 62-63 21.619342056574165 28.857705149854564 27.641080485826997 21.881872307744274 64-65 20.327752363300856 29.00281820913057 28.30366260523206 22.36576682233651 66-67 21.10740148426831 27.310313445411012 29.24964868863224 22.332636381688438 68-69 20.21114737284381 28.40880385787601 28.414642970026314 22.965405799253862 70-71 20.50323708327853 28.198197145963537 29.066717583844493 22.23184818691344 72-73 21.3478916528017 27.906209418641918 29.41394294061539 21.331955987940994 74-75 21.331380838384355 29.00445678194876 27.963709061274383 21.7004533183925 76-77 20.808859122606716 28.696278135624027 29.208997078006742 21.285865663762518 78-79 20.590266630163924 28.498041790556112 28.76830443490526 22.143387144374703 80-81 20.620863303868838 28.75319184615111 29.50709002721168 21.118854822768377 82-83 21.607211989363293 27.55218012902649 29.77247116515045 21.06813671645977 84-85 22.209629137609365 27.84542680100231 28.583570088686415 21.36137397270191 86-87 21.220145545149315 28.80943783452708 28.705197685538575 21.265218934785025 88-89 21.791822524245216 27.8355667629111 28.887551455220944 21.485059257622737 90-91 21.606130687010182 27.249724313119344 29.724556932705987 21.419588067164486 92-93 21.54141332650285 28.138128448825817 28.92778056881049 21.392677655860844 94-95 20.552425225461167 27.780607383011052 29.505651470290793 22.161315921236987 96-97 21.18574031148741 27.890243277563094 28.614398999960937 22.309617410988558 98-99 20.283063981135832 28.68976994392248 28.891501865233842 22.13566420970784 100-101 20.934145632412683 27.39545953361464 28.72324179579114 22.94715303818154 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 70.0 1 52.0 2 48.0 3 68.0 4 58.5 5 50.0 6 70.5 7 118.0 8 197.5 9 236.5 10 205.0 11 180.0 12 256.5 13 380.5 14 452.0 15 549.5 16 692.5 17 784.5 18 883.0 19 1019.0 20 1144.0 21 1262.5 22 1461.0 23 1727.0 24 1893.5 25 2167.5 26 2807.5 27 3855.5 28 6303.0 29 12773.0 30 23182.5 31 36026.5 32 52249.5 33 73613.5 34 99960.0 35 117848.5 36 129313.0 37 141431.5 38 133146.0 39 104102.0 40 78094.5 41 60240.0 42 49331.0 43 44428.0 44 41942.5 45 43447.0 46 46512.0 47 50471.0 48 56710.0 49 56540.5 50 51160.5 51 49237.5 52 47263.5 53 45287.0 54 43543.5 55 39636.0 56 35730.0 57 30456.5 58 25515.0 59 23692.5 60 20391.5 61 16145.5 62 12982.0 63 10792.0 64 8658.0 65 6160.5 66 4675.5 67 3834.0 68 3214.0 69 2723.0 70 2197.5 71 1770.5 72 1486.5 73 1228.5 74 1065.5 75 899.5 76 726.0 77 576.5 78 479.0 79 424.0 80 362.0 81 290.5 82 219.0 83 179.0 84 166.0 85 135.0 86 100.5 87 80.5 88 71.5 89 59.0 90 46.0 91 38.0 92 30.0 93 20.0 94 10.5 95 8.0 96 5.0 97 4.5 98 3.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.052473452499692E-4 2 0.002988699171218523 3 1.5196775446873846E-4 4 0.0011144301994374154 5 0.0016209893809998768 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.598387723436923E-5 12-13 5.065591815624615E-5 14-15 0.003267306721077877 16-17 0.0047869842657652616 18-19 0.0033686185573903694 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.7860754985935385E-4 42-43 2.5327959078123075E-5 44-45 4.305753043280923E-4 46-47 0.7450219162829903 48-49 0.537535275515006 50-51 0.4124658135872343 52-53 0.34625852855702055 54-55 0.3366085761482557 56-57 0.3003642667074616 58-59 0.301276073234274 60-61 0.2770878723146665 62-63 0.2724021998852137 64-65 0.2684510382690265 66-67 0.2559136985253556 68-69 0.23430894943171657 70-71 0.2478087516203562 72-73 0.8221708796349533 74-75 0.5472358838419271 76-77 0.6382392408096235 78-79 0.3610500566586444 80-81 0.31396538073241365 82-83 0.29507072326013384 84-85 0.2862312655418689 86-87 0.2579399352516054 88-89 0.2612832258499177 90-91 0.2727314633532293 92-93 0.22802761558034204 94-95 0.195709139796657 96-97 0.15095463610561355 98-99 0.16060458851437845 100-101 0.1556149805759882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1974103.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.560680462487596 #Duplication Level Percentage of deduplicated Percentage of total 1 83.78590031134885 33.984131297904604 2 8.815625793833288 7.15135561801071 3 2.8057568716251056 3.4141022377624424 4 1.2696499797351573 2.059914685089663 5 0.6808019201852753 1.3806894571441468 6 0.4401227563973901 1.0711007091902287 7 0.29047717970899717 0.8247366447474855 8 0.21902966966173376 0.7107193954363037 9 0.15609296180092871 0.5698113071445677 >10 1.1367647934775436 9.368915581465826 >50 0.18333974606000844 5.266552280159604 >100 0.18145196476005526 14.941047850357517 >500 0.020565277528902418 5.703850433469666 >1k 0.013041395506133242 9.837124036150875 >5k 0.0012539803371281963 3.1740107046381945 >10k+ 1.2539803371281963E-4 0.5419377613281193 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 10655 0.5397388079548028 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 7869 0.398611419971501 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 7409 0.37530969761962774 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 7278 0.3686737723411595 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 6162 0.3121417676787888 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 6003 0.30408747669194564 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 5745 0.29101824980763413 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 5680 0.2877256151274782 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 5577 0.2825080555573848 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 5459 0.27653065721494774 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 5222 0.2645252046119174 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 4841 0.24522529979438762 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 4544 0.23018049210198252 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 4488 0.2273437606852327 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 4063 0.20581499546882812 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 3712 0.18803476819598572 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 3671 0.18595787555157964 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 3590 0.18185474618092368 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 3480 0.1762825951837366 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 3378 0.1711156915317995 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 3367 0.1705584764320808 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 3079 0.1559695720030819 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 2948 0.14933364672461366 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 2791 0.14138066757408302 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 2781 0.14087410839252057 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 2704 0.1369736026944896 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 2657 0.13459277454114602 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 2650 0.1342381831140523 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2609 0.1321612904696462 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 2525 0.12790619334452155 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 2418 0.1224860101018032 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 2412 0.12218207459286572 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 2396 0.12137157990236577 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 2375 0.1203078056210846 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 2369 0.12000387011214712 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 2329 0.11797763338589728 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 2326 0.11782566563142856 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 2228 0.11286138565211642 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 2199 0.11139236402558528 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 2130 0.1078971056728043 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2089 0.10582021302839822 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 2018 0.10222364283930473 No Hit TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC 1995 0.10105855672171107 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 1982 0.10040002978567988 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.065591815624615E-5 2 0.0 0.0 0.0 0.0 5.065591815624615E-5 3 0.0 0.0 0.0 0.0 5.065591815624615E-5 4 0.0 0.0 0.0 0.0 5.065591815624615E-5 5 0.0 0.0 0.0 0.0 5.065591815624615E-5 6 0.0 0.0 0.0 0.0 5.065591815624615E-5 7 0.0 0.0 0.0 0.0 5.065591815624615E-5 8 0.0 0.0 0.0 0.0 5.065591815624615E-5 9 0.0 0.0 0.0 0.0 5.065591815624615E-5 10-11 0.0 0.0 0.0 2.5327959078123075E-5 5.065591815624615E-5 12-13 0.0 0.0 0.0 5.065591815624615E-5 1.5196775446873846E-4 14-15 0.0 0.0 0.0 5.065591815624615E-5 1.5196775446873846E-4 16-17 0.0 0.0 0.0 5.065591815624615E-5 1.5196775446873846E-4 18-19 0.0 0.0 0.0 5.065591815624615E-5 1.5196775446873846E-4 20-21 0.0 0.0 0.0 5.065591815624615E-5 1.7729571354686152E-4 22-23 0.0 0.0 0.0 1.013118363124923E-4 2.026236726249846E-4 24-25 0.0 0.0 0.0 2.026236726249846E-4 2.026236726249846E-4 26-27 0.0 0.0 0.0 6.33198976953077E-4 2.026236726249846E-4 28-29 0.0 0.0 0.0 0.0017223012173123694 2.026236726249846E-4 30-31 0.0 0.0 0.0 0.007041172623718215 2.026236726249846E-4 32-33 0.0 0.0 0.0 0.02330172235187323 2.026236726249846E-4 34-35 0.0 2.5327959078123075E-5 0.0 0.054404456099808374 2.026236726249846E-4 36-37 0.0 5.065591815624615E-5 0.0 0.10361668058860152 2.026236726249846E-4 38-39 0.0 5.065591815624615E-5 0.0 0.17509218110706481 2.026236726249846E-4 40-41 0.0 5.065591815624615E-5 0.0 0.2481633430474499 2.026236726249846E-4 42-43 0.0 5.065591815624615E-5 0.0 0.3303778982150374 2.026236726249846E-4 44-45 0.0 5.065591815624615E-5 0.0 0.43148711085490477 2.026236726249846E-4 46-47 0.0 5.065591815624615E-5 0.0 0.5582535460409107 2.026236726249846E-4 48-49 0.0 5.065591815624615E-5 0.0 0.6905668042650257 2.026236726249846E-4 50-51 0.0 5.065591815624615E-5 0.0 0.8242731002384374 2.026236726249846E-4 52-53 0.0 5.065591815624615E-5 0.0 0.9584606274343335 2.5327959078123077E-4 54-55 0.0 5.065591815624615E-5 0.0 1.115392661882384 2.5327959078123077E-4 56-57 0.0 5.065591815624615E-5 0.0 1.3091515488300256 2.5327959078123077E-4 58-59 0.0 5.065591815624615E-5 0.0 1.5243885450759156 2.5327959078123077E-4 60-61 0.0 5.065591815624615E-5 0.0 1.7452230202780705 2.5327959078123077E-4 62-63 0.0 5.065591815624615E-5 0.0 1.9633980597770226 2.5327959078123077E-4 64-65 0.0 5.065591815624615E-5 0.0 2.2031271924514577 2.5327959078123077E-4 66-67 0.0 5.065591815624615E-5 0.0 2.483330403732733 2.5327959078123077E-4 68-69 0.0 5.065591815624615E-5 0.0 2.791799617345194 2.5327959078123077E-4 70-71 2.5327959078123075E-5 5.065591815624615E-5 0.0 3.108475089698967 2.5327959078123077E-4 72-73 5.065591815624615E-5 5.065591815624615E-5 0.0 3.469449162480377 2.5327959078123077E-4 74-75 5.065591815624615E-5 5.065591815624615E-5 0.0 3.799523125186477 2.5327959078123077E-4 76-77 5.065591815624615E-5 5.065591815624615E-5 0.0 4.185217286028135 2.5327959078123077E-4 78-79 5.065591815624615E-5 5.065591815624615E-5 0.0 4.613918321384446 2.5327959078123077E-4 80-81 5.065591815624615E-5 5.065591815624615E-5 0.0 5.100595055070581 2.5327959078123077E-4 82-83 5.065591815624615E-5 5.065591815624615E-5 0.0 5.6179692751594015 2.5327959078123077E-4 84-85 5.065591815624615E-5 5.065591815624615E-5 0.0 6.113206859013943 2.5327959078123077E-4 86-87 5.065591815624615E-5 5.065591815624615E-5 2.5327959078123075E-5 6.651552629219448 2.5327959078123077E-4 88-89 5.065591815624615E-5 5.065591815624615E-5 5.065591815624615E-5 7.230929693131513 3.0393550893747693E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2245 0.0 60.140354 2 ACCTGGG 2350 0.0 57.85495 3 GTACCTG 2525 0.0 54.40942 1 CCTGGGG 2145 0.0 47.703793 4 CTGGGGG 1985 0.0 43.910946 5 GTACCCG 265 0.0 42.9045 1 TGGGGGG 4195 0.0 32.972763 6 GTACACG 195 0.0 31.582478 1 GGGGGGA 4170 0.0 27.036184 7 GTACATG 5830 0.0 26.002728 1 TATTCGC 350 0.0 25.71651 9 GTACTAG 1145 0.0 25.652145 1 CATGGGG 3720 0.0 25.596441 4 GGGGAAT 4250 0.0 25.525429 9 CTGTGCG 1115 0.0 25.067072 9 ATGGGGG 3185 0.0 24.987068 5 ACATGGG 5700 0.0 24.849794 3 TACATGG 6020 0.0 24.631186 2 TAGGCAT 1285 0.0 24.330793 5 CTAGGCA 1345 0.0 24.302631 4 >>END_MODULE