##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931088_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1974103 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.63704072178604 33.0 33.0 33.0 33.0 33.0 2 32.605238429808374 33.0 33.0 33.0 33.0 33.0 3 32.654422286983 33.0 33.0 33.0 33.0 33.0 4 36.59660210232192 37.0 37.0 37.0 37.0 37.0 5 36.549138520127876 37.0 37.0 37.0 37.0 37.0 6 36.55474309091268 37.0 37.0 37.0 37.0 37.0 7 36.499767742615255 37.0 37.0 37.0 37.0 37.0 8 36.50887111766711 37.0 37.0 37.0 37.0 37.0 9 36.5015001750162 37.0 37.0 37.0 37.0 37.0 10-11 36.478827345888234 37.0 37.0 37.0 37.0 37.0 12-13 36.405687291899156 37.0 37.0 37.0 37.0 37.0 14-15 38.40256562094278 40.0 37.0 40.0 37.0 40.0 16-17 38.407000799856945 40.0 37.0 40.0 37.0 40.0 18-19 38.37503488926363 40.0 37.0 40.0 37.0 40.0 20-21 38.30050939591298 40.0 37.0 40.0 37.0 40.0 22-23 38.19090366612076 40.0 37.0 40.0 35.0 40.0 24-25 38.32683907577264 40.0 37.0 40.0 37.0 40.0 26-27 38.25905588512859 40.0 37.0 40.0 37.0 40.0 28-29 38.198205970002576 40.0 37.0 40.0 37.0 40.0 30-31 38.0960147469509 40.0 37.0 40.0 35.0 40.0 32-33 37.985807478130575 40.0 37.0 40.0 33.0 40.0 34-35 37.91984587430342 40.0 37.0 40.0 33.0 40.0 36-37 37.87573470077296 40.0 37.0 40.0 33.0 40.0 38-39 37.76222922512149 40.0 37.0 40.0 33.0 40.0 40-41 37.60875825628146 40.0 37.0 40.0 33.0 40.0 42-43 37.47558663352419 40.0 37.0 40.0 33.0 40.0 44-45 37.2943957331507 40.0 37.0 40.0 33.0 40.0 46-47 37.0996548812296 40.0 37.0 40.0 33.0 40.0 48-49 36.90391585444124 40.0 37.0 40.0 33.0 40.0 50-51 36.72246356953006 40.0 37.0 40.0 33.0 40.0 52-53 36.53138868640593 37.0 37.0 40.0 33.0 40.0 54-55 36.34143051299755 37.0 37.0 40.0 33.0 40.0 56-57 36.08406754865375 37.0 37.0 40.0 33.0 40.0 58-59 35.8652441134024 37.0 37.0 40.0 33.0 40.0 60-61 35.64611269016865 37.0 37.0 40.0 30.0 40.0 62-63 35.381355734731166 37.0 33.0 40.0 27.0 40.0 64-65 35.065977560441375 37.0 33.0 40.0 27.0 40.0 66-67 34.76261421009947 37.0 33.0 40.0 27.0 40.0 68-69 34.524892318181976 37.0 33.0 40.0 27.0 40.0 70-71 34.271428086579064 37.0 33.0 37.0 27.0 40.0 72-73 33.933593130652255 37.0 33.0 37.0 27.0 40.0 74-75 33.54642868178611 37.0 33.0 37.0 27.0 40.0 76-77 31.915756675310256 33.0 33.0 37.0 24.5 37.0 78-79 32.663183228028124 37.0 33.0 37.0 27.0 37.0 80-81 32.6810888286984 37.0 33.0 37.0 27.0 37.0 82-83 32.44609779732871 37.0 33.0 37.0 24.5 37.0 84-85 32.12757186428469 37.0 33.0 37.0 22.0 37.0 86-87 31.788868919200265 33.0 33.0 37.0 22.0 37.0 88-89 31.450348841980382 33.0 33.0 37.0 22.0 37.0 90-91 31.053796331802342 33.0 33.0 37.0 22.0 37.0 92-93 30.66721113336032 33.0 33.0 37.0 15.0 37.0 94-95 30.22279713875112 33.0 33.0 37.0 15.0 37.0 96-97 29.759843078096736 33.0 30.0 37.0 4.0 37.0 98-99 29.158756660620035 33.0 27.0 37.0 2.0 37.0 100-101 27.429273447231477 33.0 24.5 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06954509828464239 1101 2 0.17253372402186784 1101 3 0.0640249334250953 1101 4 0.063380121415193 1101 5 0.12712516281676045 1101 6 0.05978203363455492 1101 7 0.007493495709717024 1101 8 -0.017396043155692098 1101 9 0.12901098840163172 1101 10-11 0.13172409106134353 1101 12-13 0.12772018989705458 1101 14-15 0.23777595798176066 1101 16-17 0.18181993871328928 1101 18-19 0.09018425282096132 1101 20-21 0.05727685183881448 1101 22-23 0.07294628214154386 1101 24-25 0.13996664670668935 1101 26-27 0.1154391700210553 1101 28-29 0.1869248589058472 1101 30-31 0.1767273234747364 1101 32-33 0.11489695615340878 1101 34-35 0.10984346882946028 1101 36-37 0.060495234012996946 1101 38-39 -0.004762437492622951 1101 40-41 -0.020221739705348796 1101 42-43 -0.06663716793728014 1101 44-45 -0.04593034046670397 1101 46-47 -0.1148130119804307 1101 48-49 -0.16902466222390444 1101 50-51 -0.11866980310920638 1101 52-53 -0.18716233888966372 1101 54-55 -0.25607614255195443 1101 56-57 -0.25999650931553475 1101 58-59 -0.2733708542163171 1101 60-61 -0.22085909888724586 1101 62-63 -0.38559319903795597 1101 64-65 -0.38407547839693024 1101 66-67 -0.41446367460070377 1101 68-69 -0.4301875774655315 1101 70-71 -0.4915885903618502 1101 72-73 -0.5549299946079529 1101 74-75 -0.6390280490059581 1101 76-77 -0.8446812083707655 1101 78-79 -0.8214188754259126 1101 80-81 -0.9913129841290669 1101 82-83 -0.9245304729229673 1101 84-85 -0.9829458294359483 1101 86-87 -1.123574370515179 1101 88-89 -1.2180722103488435 1101 90-91 -1.2889352506945215 1101 92-93 -1.3614217868399017 1101 94-95 -1.5572107221070546 1101 96-97 -1.5900181067442958 1101 98-99 -1.6814463025652806 1101 100-101 -3.2332858143604604 1102 1 0.08823852792939846 1102 2 0.24111369780095515 1102 3 0.12175442942892545 1102 4 0.12433901257319491 1102 5 0.12798206975773496 1102 6 0.14810616443656244 1102 7 0.040966127633673466 1102 8 0.12878963367157326 1102 9 0.1372548176631625 1102 10-11 0.1789593922236392 1102 12-13 0.18563956689828842 1102 14-15 0.20249496562112057 1102 16-17 0.1839091024174948 1102 18-19 0.182332476606355 1102 20-21 0.11938779276613332 1102 22-23 0.11424721121902337 1102 24-25 0.1367890945800312 1102 26-27 0.1890909361506914 1102 28-29 0.19619278493262726 1102 30-31 0.21338822280156933 1102 32-33 0.13296937251121932 1102 34-35 0.14400755122363051 1102 36-37 0.07714283379112885 1102 38-39 0.014346839415331658 1102 40-41 0.0012091278538406414 1102 42-43 0.04519044848104414 1102 44-45 -0.010832295689453986 1102 46-47 0.07705236002874472 1102 48-49 0.22477182316550426 1102 50-51 0.09134314976878244 1102 52-53 0.030652910839300773 1102 54-55 0.01024133217259049 1102 56-57 0.15779116433496654 1102 58-59 0.07989147465441704 1102 60-61 -0.029469926358686394 1102 62-63 -0.1284799889933268 1102 64-65 -0.11807804376012143 1102 66-67 -0.028093033196874728 1102 68-69 0.009726353654151865 1102 70-71 -0.08177450040395229 1102 72-73 -0.17700141507539513 1102 74-75 -0.22434165992797261 1102 76-77 -0.25776314241509013 1102 78-79 -0.06821942001406711 1102 80-81 -0.1726283788294225 1102 82-83 -0.3544801426403339 1102 84-85 -0.2711232555005445 1102 86-87 -0.3874887869738437 1102 88-89 -0.3041611030195952 1102 90-91 -0.34519675385868 1102 92-93 -0.6810383060139422 1102 94-95 -0.6433665852540678 1102 96-97 -0.7778529591256103 1102 98-99 -0.8612235823978693 1102 100-101 -1.9646382397947413 1103 1 0.07181593026167832 1103 2 0.14435897691350164 1103 3 0.11762214828454631 1103 4 0.10307395436256428 1103 5 0.061791544273127386 1103 6 0.06078057968206707 1103 7 0.09561262681035032 1103 8 0.10131592234625941 1103 9 0.01998253247437276 1103 10-11 0.07550340649463294 1103 12-13 0.13871566683377523 1103 14-15 0.10814462329057761 1103 16-17 0.1102953966773228 1103 18-19 -0.014850638938625593 1103 20-21 0.09338168522330648 1103 22-23 0.06493698800602488 1103 24-25 0.1270081789060029 1103 26-27 0.0653126854549626 1103 28-29 0.08461186540677801 1103 30-31 0.14685976025570824 1103 32-33 0.14118187503897417 1103 34-35 0.17020233778463023 1103 36-37 0.09519974684590693 1103 38-39 -0.0630485716220619 1103 40-41 -0.09177201267019797 1103 42-43 -0.08988537219745041 1103 44-45 0.024803363019984204 1103 46-47 0.014193960171262177 1103 48-49 0.03669705933024403 1103 50-51 -0.1294753981911967 1103 52-53 -0.1204242146433856 1103 54-55 -0.20647171683219057 1103 56-57 -0.22874443611569006 1103 58-59 -0.23828905776346687 1103 60-61 -0.2155197761376968 1103 62-63 -0.3541199328530382 1103 64-65 -0.34432103826164706 1103 66-67 -0.2564286495362964 1103 68-69 -0.21895773206811242 1103 70-71 -0.3951769699023444 1103 72-73 -0.6236780764137038 1103 74-75 -0.6099152080324046 1103 76-77 -0.5496592130767333 1103 78-79 -0.6147686703535058 1103 80-81 -0.6136251095967964 1103 82-83 -0.46411890206525896 1103 84-85 -0.6118549930567561 1103 86-87 -0.8342427856449639 1103 88-89 -0.7246610332053507 1103 90-91 -0.6868074888823159 1103 92-93 -0.8512839558642042 1103 94-95 -0.8696441859550994 1103 96-97 -1.0972064912449753 1103 98-99 -0.8554828652851931 1103 100-101 -1.6942377347247835 1104 1 0.05699372404224334 1104 2 0.1309849727653969 1104 3 0.12282829275036988 1104 4 0.14025412887374955 1104 5 0.0964739961530654 1104 6 0.03619805942427945 1104 7 0.10275368282793806 1104 8 0.06501716337024988 1104 9 0.13967274217403514 1104 10-11 0.09177381478539104 1104 12-13 0.09645059928508459 1104 14-15 0.21888466022824815 1104 16-17 0.2392915825106101 1104 18-19 0.14072431432136057 1104 20-21 0.07874206604933676 1104 22-23 0.17339340905004264 1104 24-25 0.15342877994962123 1104 26-27 0.10432270587744341 1104 28-29 0.09347429416092012 1104 30-31 0.12805130684465382 1104 32-33 0.14139342612586603 1104 34-35 0.1605658308253055 1104 36-37 0.0604304028416891 1104 38-39 0.17509320906449233 1104 40-41 0.08287008264534279 1104 42-43 0.10178246817546466 1104 44-45 0.20633577662195535 1104 46-47 0.12276141004075214 1104 48-49 0.06660214814194632 1104 50-51 0.1829338762084589 1104 52-53 0.17540910922458153 1104 54-55 0.168932927864212 1104 56-57 0.2919097650333242 1104 58-59 0.13133356917285965 1104 60-61 0.028747899310566538 1104 62-63 0.027044864591758255 1104 64-65 0.03995710967532062 1104 66-67 0.19594083622134661 1104 68-69 0.07342319164627753 1104 70-71 0.13042724271870298 1104 72-73 0.14451730478715064 1104 74-75 0.043370734071316974 1104 76-77 -0.07155120105495527 1104 78-79 0.06935517307255878 1104 80-81 0.006710259927579898 1104 82-83 -0.01275662665016597 1104 84-85 0.08031006336064905 1104 86-87 0.06993195498488447 1104 88-89 0.21391617357064519 1104 90-91 0.20085235287178804 1104 92-93 0.20300062095411064 1104 94-95 0.2123569907800693 1104 96-97 0.2390580788037049 1104 98-99 0.04983339933029285 1104 100-101 -0.756451359864208 1105 1 0.11582179135032789 1105 2 0.16383511715812205 1105 3 0.13494419425261128 1105 4 0.11956298035034507 1105 5 0.12744926956288083 1105 6 0.09642961982342513 1105 7 0.16175785180893598 1105 8 0.06095937648412786 1105 9 0.07744926436284771 1105 10-11 0.0941237878647101 1105 12-13 0.1787094996019576 1105 14-15 0.2108528810586776 1105 16-17 0.23031571748429513 1105 18-19 0.13216130397232462 1105 20-21 0.08484683537211168 1105 22-23 0.1252333257129905 1105 24-25 0.10630136533499268 1105 26-27 0.17691490122155074 1105 28-29 0.1596959715852151 1105 30-31 0.1217637854836795 1105 32-33 0.23586718538052054 1105 34-35 0.1661021269229792 1105 36-37 0.20891390674359656 1105 38-39 0.2664208135071462 1105 40-41 0.30904980424509887 1105 42-43 0.3140405810890954 1105 44-45 0.24823573810304822 1105 46-47 0.22083088880108193 1105 48-49 0.11240454397594846 1105 50-51 0.3545247393513833 1105 52-53 0.06823900458507381 1105 54-55 0.20517741870160222 1105 56-57 0.22115041036349226 1105 58-59 0.2496903862761144 1105 60-61 0.1500614688223152 1105 62-63 0.005518696202386764 1105 64-65 0.20002368781232605 1105 66-67 0.18636750237726574 1105 68-69 0.09753661814857395 1105 70-71 0.04136182653352449 1105 72-73 0.11634343125262347 1105 74-75 0.06508470877652428 1105 76-77 -0.07128279231180201 1105 78-79 0.016356549964193334 1105 80-81 -0.03893837946088041 1105 82-83 -0.03253773918711289 1105 84-85 0.09272656137405733 1105 86-87 0.10376821554300264 1105 88-89 0.17454521300252424 1105 90-91 0.19278146955431197 1105 92-93 0.23653947717640733 1105 94-95 0.26079027112329456 1105 96-97 0.3004063685425642 1105 98-99 0.1308579918491084 1105 100-101 -0.9484064395728247 1106 1 0.11668870802647291 1106 2 0.20026590879962924 1106 3 0.1424789203128114 1106 4 0.2063958856986332 1106 5 0.17222029623037827 1106 6 0.10756230373017672 1106 7 0.07095507148140001 1106 8 0.14993220704316457 1106 9 0.12687953430580023 1106 10-11 0.14263387661754479 1106 12-13 0.14779498928039203 1106 14-15 0.2927602205616182 1106 16-17 0.1626326731142953 1106 18-19 0.232276964059821 1106 20-21 0.2934191915432294 1106 22-23 0.24576718298758493 1106 24-25 0.24981145141791927 1106 26-27 0.36816467628244 1106 28-29 0.27221585566881856 1106 30-31 0.20390313933573623 1106 32-33 0.2684688057964948 1106 34-35 0.2521225815643078 1106 36-37 0.3109397471081081 1106 38-39 0.41326373632193025 1106 40-41 0.3492302040787081 1106 42-43 0.3994211940875516 1106 44-45 0.5214853647174635 1106 46-47 0.3789746992509677 1106 48-49 0.3538564571999032 1106 50-51 0.27902756383907246 1106 52-53 0.33472737529968555 1106 54-55 0.3117486310873758 1106 56-57 0.4362581488563535 1106 58-59 0.3581366697479851 1106 60-61 0.3449945050491223 1106 62-63 0.3420133251636486 1106 64-65 0.47584029021448515 1106 66-67 0.6349195886641823 1106 68-69 0.5059205861439366 1106 70-71 0.7117664873253418 1106 72-73 0.6602316553363039 1106 74-75 0.6279788862042182 1106 76-77 0.5011856481299297 1106 78-79 0.7804306631968032 1106 80-81 0.7467054590229552 1106 82-83 0.8373546745043541 1106 84-85 0.9651914241491824 1106 86-87 1.0774227058616752 1106 88-89 0.8793421964421952 1106 90-91 0.8846367780372795 1106 92-93 1.1642810244050779 1106 94-95 1.2223533162835913 1106 96-97 1.1669846348053312 1106 98-99 1.265792224191319 1106 100-101 0.9433608689913093 1107 1 0.04183582601277891 1107 2 0.14311174525237647 1107 3 0.038488284012174745 1107 4 0.05080540221891283 1107 5 0.05319897801575024 1107 6 0.13259698339489034 1107 7 0.07234858763102636 1107 8 0.17440532805601805 1107 9 0.12466216249649875 1107 10-11 0.10388772214232489 1107 12-13 0.08733540238804949 1107 14-15 0.1079717253742345 1107 16-17 0.09051745282405221 1107 18-19 0.21298674077570467 1107 20-21 0.19879655870410318 1107 22-23 0.15880977510992267 1107 24-25 0.20254418874291247 1107 26-27 0.22179345158074426 1107 28-29 0.14907057528066048 1107 30-31 0.13415669395389074 1107 32-33 0.150452621868574 1107 34-35 0.14892314038858956 1107 36-37 0.25959371532511 1107 38-39 0.36782723587037225 1107 40-41 0.3806560946669322 1107 42-43 0.27982388582198325 1107 44-45 0.273210992932718 1107 46-47 0.30873745970924205 1107 48-49 0.3408287015685616 1107 50-51 0.31158429379544117 1107 52-53 0.32822957456894386 1107 54-55 0.4966494126868852 1107 56-57 0.47365091659868597 1107 58-59 0.4726669112511175 1107 60-61 0.4951319451366629 1107 62-63 0.40203326074897916 1107 64-65 0.591365538706718 1107 66-67 0.6556800423772344 1107 68-69 0.7025026991590124 1107 70-71 0.5208627500740235 1107 72-73 0.6266825768064521 1107 74-75 0.5741588822090051 1107 76-77 0.5483299907303341 1107 78-79 0.7382569248492103 1107 80-81 0.6736247274978666 1107 82-83 0.8552639141696332 1107 84-85 0.8746211028530695 1107 86-87 0.8663224787823474 1107 88-89 0.8978465414326422 1107 90-91 0.8321301579494289 1107 92-93 0.9472941790051124 1107 94-95 1.0683469953307956 1107 96-97 1.0664415837898247 1107 98-99 1.13599609428746 1107 100-101 1.8002318986159302 1108 1 -0.013932048350397963 1108 2 0.03592199267329477 1108 3 0.045776320439635754 1108 4 0.11687458017280505 1108 5 -0.02665283306649968 1108 6 1.91526675628495E-4 1108 7 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-0.27121248139285825 2316 50-51 -0.35799208983716113 2316 52-53 -0.32848363115434864 2316 54-55 -0.17750077836338818 2316 56-57 -0.239994359124708 2316 58-59 -0.13354461651272942 2316 60-61 -0.08323411904528655 2316 62-63 -0.1519010526099578 2316 64-65 -0.15604034965425484 2316 66-67 0.013755564231750839 2316 68-69 0.1486216199757422 2316 70-71 0.019144893484842385 2316 72-73 0.030586454351883674 2316 74-75 -0.04439613479857485 2316 76-77 -0.03882529803372492 2316 78-79 0.1683851884347547 2316 80-81 0.1519750608099244 2316 82-83 0.1575638129449004 2316 84-85 0.05550159839827273 2316 86-87 0.2741659133100107 2316 88-89 0.26591424344893255 2316 90-91 0.27906856589420315 2316 92-93 0.38472601190223443 2316 94-95 0.3702458709155714 2316 96-97 0.4980873063611142 2316 98-99 0.5970947929569483 2316 100-101 1.0193522010134082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 11.0 5 27.0 6 44.0 7 124.0 8 360.0 9 630.0 10 1108.0 11 1731.0 12 2419.0 13 3360.0 14 4154.0 15 5168.0 16 6370.0 17 8291.0 18 8620.0 19 7081.0 20 6829.0 21 7199.0 22 7881.0 23 9173.0 24 10490.0 25 12898.0 26 15530.0 27 19342.0 28 23876.0 29 29860.0 30 38164.0 31 48750.0 32 64158.0 33 86897.0 34 124810.0 35 190208.0 36 313319.0 37 554683.0 38 360503.0 39 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.70349760397802 25.269615845583836 14.627597549426191 23.399289001011947 2 12.617135597418775 23.091878277757825 43.33339580902902 20.95759031579438 3 15.803987937812769 31.85355576684702 30.93592380944662 21.406532485893592 4 10.392162921590211 19.050221796937645 42.51323259222037 28.04438268925178 5 11.09065737704669 39.0468987687066 37.071064681022214 12.791379173224499 6 25.050010055199756 42.44246627455609 20.611031947167906 11.896491723076252 7 23.300759889428264 33.24340219329994 27.57880414547772 15.877033771794075 8 22.761274361064242 38.251904789162474 21.809348347072063 17.177472502701228 9 25.169406054294026 15.885391998289855 24.01450177624977 34.93070017116634 10-11 22.394728137285643 28.164589183036547 30.676286900936777 18.764395778741026 12-13 22.745874961944743 26.0201468717691 30.595237431886783 20.63874073439937 14-15 20.536812663413365 27.324626734306044 27.926497007618423 24.212063594662165 16-17 19.092063585334706 29.721929402873105 29.583486778552082 21.60252023324011 18-19 18.900432246949627 30.47049723342703 30.982375286395897 19.646695233227447 20-21 19.714143588252487 28.80890713402492 30.366677929165803 21.11027134855679 22-23 19.913499954156393 29.699260879498183 29.57502724021999 20.812211926125435 24-25 19.91043527110794 29.36275361518624 30.08832365889723 20.638487454808587 26-27 20.079322102240866 29.0305267759585 29.827192400801785 21.062958720998854 28-29 19.525171685570612 30.37875936564607 29.214230463152123 20.881838485631196 30-31 20.835817583986245 29.483719947743353 28.936408080024194 20.744054388246205 32-33 20.893540002725288 29.512771116805965 29.001982166077454 20.591706714391293 34-35 21.088058728445276 28.470981504004605 29.888004830548354 20.552954937001765 36-37 21.119364085865833 29.125734574133162 29.71934595104713 20.035555388953867 38-39 21.5305237520655 28.41757923349452 30.545128873786666 19.506768140653318 40-41 20.356740220507017 28.846961930638237 30.50298081372656 20.29331703512819 42-43 21.066130831056007 28.629650071416325 29.747539056078594 20.556680041449077 44-45 20.419476592660057 29.78661701035863 29.551801501745352 20.24210489523596 46-47 21.69519523550696 28.858068702595556 28.671654923780572 20.775081138116906 48-49 21.50066397362443 27.88367556954449 29.75427530375242 20.861385153078658 50-51 21.145401227798143 28.60433320855092 28.604383864469078 21.645881699181857 52-53 20.314137610854143 28.786314594527234 29.22063843679889 21.678909357819727 54-55 20.64375567029684 29.426453432267717 28.61874481726637 21.311046080169067 56-57 21.411572749750142 28.355410026731125 29.067024364990075 21.16599285852866 58-59 21.234732939466685 27.927823421574256 29.91193975187718 20.92550388708188 60-61 21.114957020986242 29.797153441335126 28.588022002904612 20.499867534774022 62-63 21.501056429173147 28.932608886162477 27.81364498205007 21.7526897026143 64-65 20.170604066758422 29.17965779901049 28.3975810785962 22.25215705563489 66-67 21.05137847563368 27.32471094272602 29.273778505217585 22.350132076422717 68-69 20.025861785096417 28.595712668221918 28.452421661081434 22.92600388560023 70-71 20.40560252128148 28.374547984613557 29.036250133290242 22.18359936081472 72-73 21.30468876244046 28.07199016464693 29.312274992743543 21.311046080169067 74-75 21.140968834959473 29.195791708943254 27.973869651178283 21.689369804918993 76-77 20.419549139937434 28.933957052720732 29.294151706257853 21.35234210108398 78-79 20.30838757361603 28.85819445144514 28.689704887438484 22.143713087500338 80-81 20.248341137633716 28.976273525298772 29.58254950498184 21.192835832085667 82-83 21.196523265026578 27.83110338413281 29.81445120555046 21.157922145290158 84-85 21.8819534659652 28.139132513922444 28.468023143161012 21.51089087695134 86-87 20.896044447630146 29.044574016950016 28.563240385421494 21.49614114999834 88-89 21.208163910393733 28.228770231340512 28.652836250185526 21.910229608080225 90-91 20.976894316051393 27.62355864916876 29.51046627252985 21.889080762249993 92-93 20.946647606330437 28.37534104459693 28.646606371974414 22.031404977098216 94-95 19.636738411535376 28.2076227740989 29.43949236891803 22.716146445447695 96-97 20.34906516774702 28.236908599808313 28.364691972418044 23.049334260026626 98-99 19.35078868731773 28.972829685178535 28.714585814417994 22.961795813085743 100-101 18.457069044121372 28.60592168583152 28.70804417202776 24.22896509801935 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 66.0 1 48.5 2 48.5 3 66.5 4 53.5 5 46.5 6 75.0 7 148.0 8 220.5 9 236.5 10 225.0 11 249.0 12 307.0 13 340.0 14 405.0 15 487.5 16 566.0 17 685.5 18 777.5 19 863.0 20 972.0 21 1089.0 22 1227.0 23 1394.5 24 1622.0 25 1941.0 26 2534.5 27 3747.0 28 6823.0 29 14519.5 30 25848.0 31 39519.5 32 57424.0 33 79408.5 34 106639.0 35 123810.5 36 130928.5 37 139081.5 38 127440.5 39 97453.5 40 72750.0 41 56709.0 42 48440.5 43 44606.5 44 42401.5 45 44939.0 46 48159.5 47 52339.0 48 59532.0 49 58800.5 50 52627.5 51 50636.0 52 48711.5 53 46565.5 54 44633.0 55 40552.5 56 35662.0 57 29938.0 58 25131.5 59 23452.5 60 19690.0 61 14582.0 62 10959.5 63 8598.5 64 6504.5 65 4254.0 66 2983.5 67 2227.0 68 1796.5 69 1488.5 70 1179.5 71 854.0 72 631.0 73 514.5 74 418.0 75 319.5 76 257.0 77 213.5 78 163.5 79 138.0 80 97.0 81 60.0 82 51.5 83 55.5 84 53.5 85 42.0 86 27.0 87 15.0 88 11.0 89 9.0 90 5.0 91 5.0 92 4.0 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08444341556646234 2 1.013118363124923E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.2926346801559996E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.065591815624615E-5 40-41 0.0031153389666091383 42-43 0.005800102628890184 44-45 0.0 46-47 0.0 48-49 1.7729571354686152E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0028620593758279076 68-69 0.007497075887124431 70-71 0.0013930377492967691 72-73 0.0 74-75 0.0 76-77 0.0015956614219217538 78-79 0.004736328347609016 80-81 0.0042804250842028 82-83 0.0033939465164684922 84-85 0.0021528765216404615 86-87 2.2795163170310768E-4 88-89 0.0 90-91 0.0 92-93 0.0010637742812811693 94-95 0.003596570189093477 96-97 0.002988699171218523 98-99 0.0 100-101 1.5196775446873846E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1974103.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.59826053780667 #Duplication Level Percentage of deduplicated Percentage of total 1 80.46846626655324 28.645374272344696 2 10.758094718577935 7.659389173646786 3 3.3040880208387184 3.5285935861698805 4 1.4754333814348441 2.1009144767397867 5 0.8113624715389252 1.4441546326220704 6 0.5288415590294865 1.1295503760930914 7 0.34571315461135005 0.8614750864441314 8 0.25712269178522046 0.7322496457881953 9 0.193339104296948 0.6194282226218039 >10 1.3640942202748019 9.860118582517607 >50 0.21945336820916886 5.528563070634694 >100 0.2310682325652264 17.03319572428507 >500 0.02546753410195533 6.299035830947116 >1k 0.015738363770871268 10.333150786314988 >5k 0.0015738363770871268 3.645804969638126 >10k+ 1.430760342806479E-4 0.5790015631919952 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 11368 0.5758564776002063 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 9240 0.4680606837637145 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 7885 0.3994219146620009 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 7113 0.3603155458453789 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 6744 0.34162351204572405 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 6089 0.3084438856533828 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 6034 0.30565781015478927 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 6002 0.3040368207737894 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 5782 0.29289251877941525 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 5689 0.28818151839088435 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 5568 0.2820521522939786 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 5435 0.27531491517919787 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 4872 0.24679563325723125 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 4456 0.22572277130423285 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 3834 0.19421479021104773 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 3632 0.183982294743486 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 3630 0.18388098290717353 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 3390 0.17172356254967447 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 3371 0.17076110010470577 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 3333 0.16883617521476843 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 3145 0.15931286260139416 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 3133 0.1587049915835192 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 3095 0.15678006669358185 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 3090 0.15652678710280063 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 3088 0.15642547526648812 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 3031 0.15353808793158208 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 2962 0.1500428295788011 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2846 0.14416674307267655 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 2758 0.13970902227492688 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 2756 0.1396077104386144 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 2715 0.1375308177942083 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 2676 0.1355552369861147 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 2607 0.1320599786333337 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 2595 0.13145210761545878 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 2540 0.12866603211686523 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 2383 0.12071305296633458 No Hit TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC 2336 0.11833222481299102 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 2313 0.11716713869539734 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 2265 0.11473565462389755 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2253 0.11412778360602258 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 2213 0.11210154687977274 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2139 0.10835300893621053 No Hit CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT 2110 0.10688398730967939 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 2107 0.10673201955521065 No Hit ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT 2029 0.10278085793902345 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 2003 0.10146380406696105 No Hit ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA 2001 0.10136249223064855 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 1986 0.10060265345830485 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 2.5327959078123075E-5 0.0 0.0 0.0 22-23 0.0 5.065591815624615E-5 0.0 5.065591815624615E-5 0.0 24-25 0.0 5.065591815624615E-5 0.0 1.2663979539061538E-4 0.0 26-27 0.0 5.065591815624615E-5 0.0 3.799193861718462E-4 0.0 28-29 0.0 5.065591815624615E-5 0.0 0.0014690216265311384 0.0 30-31 0.0 5.065591815624615E-5 0.0 0.0066865811966244915 0.0 32-33 0.0 5.065591815624615E-5 0.0 0.022795163170310766 0.0 34-35 0.0 5.065591815624615E-5 0.0 0.053847241000089655 0.0 36-37 0.0 5.065591815624615E-5 0.0 0.10331274507966404 0.0 38-39 0.0 5.065591815624615E-5 0.0 0.17491488539351796 0.0 40-41 0.0 5.065591815624615E-5 0.0 0.2480367032520593 0.0 42-43 0.0 5.065591815624615E-5 0.0 0.3306058498467405 0.0 44-45 0.0 5.065591815624615E-5 0.0 0.432778836767889 0.0 46-47 0.0 5.065591815624615E-5 0.0 0.5596719117492857 0.0 48-49 0.0 5.065591815624615E-5 0.0 0.6919598420143225 0.0 50-51 0.0 5.065591815624615E-5 0.0 0.8244250679929062 0.0 52-53 0.0 5.065591815624615E-5 0.0 0.9578527564164585 0.0 54-55 0.0 5.065591815624615E-5 0.0 1.1135690488287593 0.0 56-57 0.0 5.065591815624615E-5 0.0 1.305352354968307 0.0 58-59 0.0 5.065591815624615E-5 0.0 1.5179805714291503 0.0 60-61 0.0 5.065591815624615E-5 0.0 1.7369661056186025 0.0 62-63 0.0 5.065591815624615E-5 0.0 1.9542799945088984 0.0 64-65 0.0 5.065591815624615E-5 0.0 2.1927174012703494 0.0 66-67 0.0 5.065591815624615E-5 0.0 2.4716542145977183 0.0 68-69 0.0 5.065591815624615E-5 0.0 2.7778439118931484 0.0 70-71 0.0 5.065591815624615E-5 0.0 3.092569131397906 0.0 72-73 0.0 5.065591815624615E-5 0.0 3.450326553376394 0.0 74-75 0.0 5.065591815624615E-5 0.0 3.7783742793562443 0.0 76-77 0.0 5.065591815624615E-5 0.0 4.161333020617466 0.0 78-79 0.0 5.065591815624615E-5 0.0 4.584968464158152 0.0 80-81 0.0 5.065591815624615E-5 0.0 5.06741542867824 0.0 82-83 0.0 5.065591815624615E-5 0.0 5.579065530015404 0.0 84-85 0.0 5.065591815624615E-5 0.0 6.068553667159211 0.0 86-87 0.0 5.065591815624615E-5 0.0 6.599478345354827 0.0 88-89 0.0 5.065591815624615E-5 0.0 7.170167919303097 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2395 0.0 61.67822 2 ACCTGGG 2415 0.0 60.57739 3 GTACCTG 2670 0.0 55.538654 1 CCTGGGG 2090 0.0 55.452415 4 CTGGGGG 1715 0.0 54.837543 5 GTACCCG 210 0.0 38.475266 1 TGGGGGG 3735 0.0 37.13378 6 TAACGCA 150 2.5465852E-11 34.831997 4 GGGGGGA 3835 0.0 29.972773 7 ATAACGC 210 1.8189894E-12 29.403633 3 CATGGGG 3730 0.0 26.35958 4 GTACACG 235 5.456968E-12 26.292236 1 ATGGGGG 2975 0.0 26.18387 5 GGGGAAT 4430 0.0 25.41099 9 CTGTGCG 1380 0.0 25.125847 9 GGGGGAA 4525 0.0 24.982466 8 TATTCTC 1710 0.0 24.165737 5 ACATGGG 5605 0.0 24.151615 3 GTACATG 5835 0.0 23.947407 1 TAGTACT 1585 0.0 23.374496 4 >>END_MODULE