##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931087_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2298732 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.795547719351365 33.0 33.0 33.0 27.0 33.0 2 30.709277114513565 33.0 33.0 33.0 27.0 33.0 3 30.739027864057228 33.0 33.0 33.0 27.0 33.0 4 34.30699272468474 37.0 37.0 37.0 27.0 37.0 5 34.277411198869636 37.0 37.0 37.0 27.0 37.0 6 34.373772584189894 37.0 37.0 37.0 33.0 37.0 7 34.33051003770775 37.0 37.0 37.0 27.0 37.0 8 34.32830882416915 37.0 37.0 37.0 27.0 37.0 9 34.348081899064354 37.0 37.0 37.0 33.0 37.0 10-11 34.23861655034167 37.0 37.0 37.0 27.0 37.0 12-13 34.149627707797165 37.0 37.0 37.0 27.0 37.0 14-15 35.72707366495963 40.0 37.0 40.0 27.0 40.0 16-17 35.66144879002859 40.0 37.0 40.0 27.0 40.0 18-19 35.592208226100304 40.0 37.0 40.0 27.0 40.0 20-21 35.49884458040346 40.0 37.0 40.0 27.0 40.0 22-23 35.40097671237882 40.0 37.0 40.0 27.0 40.0 24-25 35.27350513239473 40.0 37.0 40.0 27.0 40.0 26-27 35.09295494211591 38.5 37.0 40.0 27.0 40.0 28-29 34.97010525802921 37.0 37.0 40.0 27.0 40.0 30-31 34.82517492252251 37.0 37.0 40.0 27.0 40.0 32-33 34.66169718784095 37.0 37.0 40.0 22.0 40.0 34-35 34.540741373940065 37.0 37.0 40.0 22.0 40.0 36-37 34.322662885451635 37.0 35.0 40.0 22.0 40.0 38-39 34.13833191516018 37.0 33.0 40.0 22.0 40.0 40-41 33.91370155372614 37.0 33.0 40.0 18.5 40.0 42-43 33.72985193576285 37.0 33.0 40.0 15.0 40.0 44-45 33.4718901550942 37.0 33.0 40.0 15.0 40.0 46-47 33.32969893837124 37.0 33.0 40.0 15.0 40.0 48-49 33.32784639531707 37.0 33.0 40.0 15.0 40.0 50-51 32.551784201029086 37.0 33.0 38.5 10.5 40.0 52-53 32.67219775946043 37.0 33.0 38.5 6.0 40.0 54-55 33.16348882775374 37.0 33.0 40.0 6.0 40.0 56-57 33.11830609222824 37.0 33.0 40.0 6.0 40.0 58-59 32.96927610526151 37.0 33.0 40.0 6.0 40.0 60-61 32.98858675130464 37.0 33.0 40.0 6.0 40.0 62-63 32.81766665274595 37.0 33.0 40.0 6.0 40.0 64-65 32.59952965373954 37.0 33.0 40.0 2.0 40.0 66-67 32.36102316407481 37.0 33.0 40.0 2.0 40.0 68-69 32.13311860625771 37.0 33.0 40.0 2.0 40.0 70-71 31.889138881783524 37.0 33.0 40.0 2.0 40.0 72-73 31.291431754549897 37.0 33.0 37.0 2.0 40.0 74-75 31.11039455665123 37.0 33.0 37.0 2.0 40.0 76-77 30.75527616964483 37.0 33.0 37.0 2.0 40.0 78-79 30.487405882895438 37.0 33.0 37.0 2.0 37.0 80-81 30.222424362648624 37.0 33.0 37.0 2.0 37.0 82-83 29.94284022669889 37.0 33.0 37.0 2.0 37.0 84-85 29.54942072412095 35.0 27.0 37.0 2.0 37.0 86-87 29.3734152132567 33.0 27.0 37.0 2.0 37.0 88-89 29.166507665965412 33.0 27.0 37.0 2.0 37.0 90-91 28.88049629099869 33.0 27.0 37.0 2.0 37.0 92-93 28.595370404205447 33.0 27.0 37.0 2.0 37.0 94-95 28.335335524106334 33.0 27.0 37.0 2.0 37.0 96-97 28.031036240849303 33.0 27.0 37.0 2.0 37.0 98-99 27.695087552615963 33.0 27.0 37.0 2.0 37.0 100-101 26.99790688953736 33.0 24.5 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.008368629415766549 1101 2 0.05241459746905974 1101 3 0.02388541845255432 1101 4 0.15571726511842598 1101 5 -0.04978847697354638 1101 6 0.012422099890159188 1101 7 0.22089873731646748 1101 8 0.19858710454796835 1101 9 0.18266689131990432 1101 10-11 0.18552513871763665 1101 12-13 0.21154413331496613 1101 14-15 0.28810513255318426 1101 16-17 0.14721853944229935 1101 18-19 0.159295779971238 1101 20-21 0.1114935406187314 1101 22-23 0.2131917277427675 1101 24-25 0.23792977319063624 1101 26-27 0.16910663706007512 1101 28-29 0.09211951717599476 1101 30-31 0.16559517776760657 1101 32-33 0.12144530689090516 1101 34-35 0.11207953414106697 1101 36-37 0.21678398903379303 1101 38-39 0.1600267269950777 1101 40-41 0.12727717600805022 1101 42-43 -0.0375944594157005 1101 44-45 0.1394284013750564 1101 46-47 0.30829636639276004 1101 48-49 0.33908230752805224 1101 50-51 0.20511701522628556 1101 52-53 0.2669879444796095 1101 54-55 0.28472784986301036 1101 56-57 0.31622657075457283 1101 58-59 0.24875341852570898 1101 60-61 0.22710071799985343 1101 62-63 0.19941839100989966 1101 64-65 0.25400401631176095 1101 66-67 0.20414011655993392 1101 68-69 0.29155612199575387 1101 70-71 0.2502658735419807 1101 72-73 0.3670134888269274 1101 74-75 0.24163002122809374 1101 76-77 0.19867279863769127 1101 78-79 0.20079105203076963 1101 80-81 0.21925265839952957 1101 82-83 0.2961198707730013 1101 84-85 0.3693346866003431 1101 86-87 0.22366171309824523 1101 88-89 0.24822571180361663 1101 90-91 0.27631036282292243 1101 92-93 0.18148040346499172 1101 94-95 0.2270793168334535 1101 96-97 0.26277936919305134 1101 98-99 0.252239376912307 1101 100-101 0.4004969030555614 1102 1 -0.03549454514527284 1102 2 -0.014384914269221838 1102 3 -0.06286422030480665 1102 4 -0.05910013210266385 1102 5 0.017037204994608146 1102 6 -0.04802879447744601 1102 7 0.09449512507702451 1102 8 -0.01634525475433435 1102 9 -0.007359821007582923 1102 10-11 0.041485128174770125 1102 12-13 0.005238136032936325 1102 14-15 0.23802232246866595 1102 16-17 0.23897790791726692 1102 18-19 0.22063155523625966 1102 20-21 0.1675644264249314 1102 22-23 0.1628659083809083 1102 24-25 0.19929129809254675 1102 26-27 0.16494370120891944 1102 28-29 0.25260634887711575 1102 30-31 0.26362441926087854 1102 32-33 0.24537863636101065 1102 34-35 0.13490885377117223 1102 36-37 0.21013511500792958 1102 38-39 0.13086280976030196 1102 40-41 0.13631104589340737 1102 42-43 0.15212031311826735 1102 44-45 0.263911772997929 1102 46-47 0.3461611840703753 1102 48-49 0.2541944936955858 1102 50-51 0.09524434274109694 1102 52-53 0.3597579044204693 1102 54-55 0.37437467326443397 1102 56-57 0.34941603013370326 1102 58-59 0.5313402616295733 1102 60-61 0.4436757265519091 1102 62-63 0.4327119705178575 1102 64-65 0.4359039376141496 1102 66-67 0.5475213382984307 1102 68-69 0.482616792065798 1102 70-71 0.46522603871352075 1102 72-73 0.39038677537785915 1102 74-75 0.41082499691782104 1102 76-77 0.46247689390480673 1102 78-79 0.4381875160588571 1102 80-81 0.508625535573298 1102 82-83 0.4177894753595197 1102 84-85 0.5865211906127961 1102 86-87 0.4593762725317774 1102 88-89 0.5597956171608516 1102 90-91 0.6132782426043626 1102 92-93 0.435912285160601 1102 94-95 0.33549486830562003 1102 96-97 0.5327409456876993 1102 98-99 0.49984106227511305 1102 100-101 0.5659649576167531 1103 1 0.09586232491713176 1103 2 0.17936762645063808 1103 3 0.19769657664130236 1103 4 0.12135160237678377 1103 5 0.13113834350164666 1103 6 0.15188716887092824 1103 7 0.3215991056630614 1103 8 0.17210274789815827 1103 9 0.20242978754740193 1103 10-11 0.24015487118839474 1103 12-13 0.32252697961629906 1103 14-15 0.40578258256783784 1103 16-17 0.46512049333615124 1103 18-19 0.38686473798555454 1103 20-21 0.4578934215388131 1103 22-23 0.4438503452868474 1103 24-25 0.4090716476250549 1103 26-27 0.5044932189020273 1103 28-29 0.5050220719318119 1103 30-31 0.45235768798681164 1103 32-33 0.49460198073756345 1103 34-35 0.6124828399743478 1103 36-37 0.5450055195566677 1103 38-39 0.6348988617483897 1103 40-41 0.6227884640194006 1103 42-43 0.5580343153175065 1103 44-45 0.5079374989714225 1103 46-47 0.3640593154232761 1103 48-49 0.346075521429448 1103 50-51 0.48112182030119754 1103 52-53 0.7155184272995072 1103 54-55 0.7788469133923286 1103 56-57 0.7579953080886739 1103 58-59 0.6913258087363872 1103 60-61 0.8499091650265882 1103 62-63 0.7837047581802565 1103 64-65 0.8734408083643572 1103 66-67 0.7519471069516115 1103 68-69 0.812558751222042 1103 70-71 0.7926468983211628 1103 72-73 0.6805960645582978 1103 74-75 0.7370946513975589 1103 76-77 0.5712538740349444 1103 78-79 0.805186949053958 1103 80-81 0.8153639434976085 1103 82-83 0.8701533478883512 1103 84-85 0.7932202697368744 1103 86-87 0.77199900969282 1103 88-89 0.737716772616313 1103 90-91 0.7019878346871025 1103 92-93 0.7258925237611997 1103 94-95 0.7841714453596502 1103 96-97 0.877321639832207 1103 98-99 0.9617836140087981 1103 100-101 0.9500327371885255 1104 1 0.12445321601352788 1104 2 0.16022015073534845 1104 3 0.12184785020647126 1104 4 0.25573738115338784 1104 5 0.05470457679120955 1104 6 0.01818153338007278 1104 7 0.04120073482987863 1104 8 0.042905831158648766 1104 9 0.08391931388424467 1104 10-11 -0.037648755099830566 1104 12-13 0.09855980875736492 1104 14-15 0.19609842070961747 1104 16-17 0.1680592390549478 1104 18-19 0.19129323578020774 1104 20-21 0.27469074511018476 1104 22-23 0.28599648788785714 1104 24-25 0.2894806574543125 1104 26-27 0.3492822846058701 1104 28-29 0.27682271261257796 1104 30-31 0.18388293543230105 1104 32-33 0.3418149190945172 1104 34-35 0.4030965282028447 1104 36-37 0.45961388536088776 1104 38-39 0.40826170000680406 1104 40-41 0.5113038036123854 1104 42-43 0.40817078291162545 1104 44-45 0.5205275698827307 1104 46-47 0.3908787823197031 1104 48-49 0.3239848688221372 1104 50-51 0.3556727982318648 1104 52-53 0.5003564365656814 1104 54-55 0.6127387417654617 1104 56-57 0.644132340866399 1104 58-59 0.48302137208691676 1104 60-61 0.5108349541620996 1104 62-63 0.5488589525574383 1104 64-65 0.48987427408945194 1104 66-67 0.579746699200065 1104 68-69 0.5665477476992606 1104 70-71 0.5658006144660668 1104 72-73 0.5380584946889364 1104 74-75 0.5682436462527996 1104 76-77 0.6184041698157579 1104 78-79 0.6078202991505002 1104 80-81 0.6633614294338592 1104 82-83 0.5450821669430645 1104 84-85 0.6442811176878571 1104 86-87 0.6010822835606433 1104 88-89 0.5857895048902613 1104 90-91 0.586557444447056 1104 92-93 0.5531105245245342 1104 94-95 0.5260158919500064 1104 96-97 0.3852088146373909 1104 98-99 0.6588701803219301 1104 100-101 0.8002269981503538 1105 1 -0.01293681963972304 1105 2 -0.06819769279590204 1105 3 -0.0682216177614734 1105 4 -0.02984862144419509 1105 5 -0.1237833495210765 1105 6 -0.12770888446227247 1105 7 -0.006501076090266622 1105 8 -0.11953724690310708 1105 9 0.01243014789594099 1105 10-11 -0.03365779048763784 1105 12-13 0.002313774745196895 1105 14-15 -0.05289556180722599 1105 16-17 -0.08842304871186712 1105 18-19 0.025710472774697735 1105 20-21 0.05814441037165352 1105 22-23 -0.06539077283014194 1105 24-25 -0.09107239057026817 1105 26-27 -0.08928576840520464 1105 28-29 -0.18209801847113027 1105 30-31 -0.11903990966258249 1105 32-33 -0.30314470719370945 1105 34-35 -0.1915314832728754 1105 36-37 -0.1989322235520703 1105 38-39 -0.22966008985599018 1105 40-41 -0.2681464825417166 1105 42-43 -0.2111818780171859 1105 44-45 -0.16541555635907912 1105 46-47 -0.19817571199357076 1105 48-49 -0.22145986864380518 1105 50-51 -0.13345180842076587 1105 52-53 -0.20464766908522591 1105 54-55 -0.08478829079775352 1105 56-57 -0.029624776038538414 1105 58-59 -0.05145456773470869 1105 60-61 -0.05675428684522643 1105 62-63 -0.11503396118961007 1105 64-65 -0.08245050565671619 1105 66-67 -0.037296570044908606 1105 68-69 -0.08961330022601288 1105 70-71 -0.1325094180421793 1105 72-73 -0.17113964124505898 1105 74-75 -0.09109166985276218 1105 76-77 -0.19339267465603882 1105 78-79 -0.0991092355837786 1105 80-81 -0.07436019147356987 1105 82-83 -0.10753247353019901 1105 84-85 -7.97924220524493E-4 1105 86-87 -0.05510950501932399 1105 88-89 -0.17592903958703587 1105 90-91 -0.010092060657960644 1105 92-93 -0.026123808530361003 1105 94-95 -0.04942729074820562 1105 96-97 0.045840558737992865 1105 98-99 -0.028192319567228452 1105 100-101 0.015592182348942885 1106 1 0.14211744217679723 1106 2 0.09021325041362971 1106 3 0.1068165000435215 1106 4 0.0921740669677007 1106 5 0.07367179876948171 1106 6 0.18685168306627276 1106 7 0.1194378370532192 1106 8 0.10270503478385251 1106 9 0.12243625253112356 1106 10-11 0.138034929373255 1106 12-13 0.050934445444973164 1106 14-15 0.07514265147310795 1106 16-17 0.23219817748078242 1106 18-19 0.16781451774647138 1106 20-21 0.20112943231651315 1106 22-23 0.13991100156452774 1106 24-25 0.18562902333267317 1106 26-27 0.16918448524094032 1106 28-29 0.17683167406935496 1106 30-31 0.24175184126830374 1106 32-33 0.3359834004561719 1106 34-35 0.17812124688779107 1106 36-37 0.22041975929708713 1106 38-39 0.1545595728556819 1106 40-41 0.29742250706882345 1106 42-43 0.2591793948715875 1106 44-45 0.16817324583048787 1106 46-47 0.1584738559081984 1106 48-49 0.2119839846250713 1106 50-51 0.1456341743531695 1106 52-53 0.25782386913818556 1106 54-55 0.38098322225487635 1106 56-57 0.38549215231734735 1106 58-59 0.41917577201282796 1106 60-61 0.26765268111846297 1106 62-63 0.3705952646789967 1106 64-65 0.4436486903252117 1106 66-67 0.41913976960425714 1106 68-69 0.5330811873752168 1106 70-71 0.5374910738004637 1106 72-73 0.20403214203171416 1106 74-75 0.42079153777137535 1106 76-77 0.376401821914083 1106 78-79 0.4526586494060503 1106 80-81 0.5259383859439097 1106 82-83 0.6215840324196513 1106 84-85 0.5684648036545106 1106 86-87 0.6447105384364207 1106 88-89 0.633376333938525 1106 90-91 0.6044439147383329 1106 92-93 0.7986977538512008 1106 94-95 0.7155112723190378 1106 96-97 0.6198478322227565 1106 98-99 0.5282046726703413 1106 100-101 0.46151350488488774 1107 1 -0.0145821185077466 1107 2 0.012710135863763128 1107 3 -0.06434167537667079 1107 4 -0.018924658798916028 1107 5 0.039654357290238806 1107 6 -0.08622713143768834 1107 7 -0.17663946935292074 1107 8 -0.03960967681975802 1107 9 0.013854848033055589 1107 10-11 -0.1142209336302642 1107 12-13 -0.26674882122606647 1107 14-15 -0.1053550754519108 1107 16-17 -0.19791760727650143 1107 18-19 -0.21786702226183507 1107 20-21 -0.018162745245611234 1107 22-23 -0.11978863561773068 1107 24-25 -0.1713524860765432 1107 26-27 -0.14557602226675925 1107 28-29 -0.15447884108812815 1107 30-31 -0.1004858262070556 1107 32-33 -0.15241927259258148 1107 34-35 -0.09277191858770095 1107 36-37 -0.05848517589841862 1107 38-39 -0.20676864605892575 1107 40-41 -0.0057248275321839515 1107 42-43 -0.003548459039635077 1107 44-45 -0.09732245936917394 1107 46-47 -0.22104633948882935 1107 48-49 -0.16959645818742075 1107 50-51 -0.24096205977355822 1107 52-53 -0.08472252126244229 1107 54-55 -0.011240511707917733 1107 56-57 -0.009090170153307042 1107 58-59 0.04126421430076732 1107 60-61 0.03649652742362974 1107 62-63 0.06629035571127417 1107 64-65 0.0049741771785392075 1107 66-67 0.04143311648861214 1107 68-69 0.042728095774670294 1107 70-71 -0.016033558464087605 1107 72-73 0.032996909369373384 1107 74-75 0.2032721947774725 1107 76-77 0.09805204157180825 1107 78-79 -0.009043315492423432 1107 80-81 0.27555562415779633 1107 82-83 0.26196526425144384 1107 84-85 0.2791575451140922 1107 86-87 0.271692375692993 1107 88-89 0.11522451875700668 1107 90-91 0.24761321511208223 1107 92-93 0.001597017287476632 1107 94-95 0.1115997801764479 1107 96-97 0.26684966816794287 1107 98-99 0.1908019340521072 1107 100-101 0.18774921620436302 1108 1 0.11815671492384894 1108 2 0.05413436042174524 1108 3 0.06354848530264334 1108 4 0.17840458131195192 1108 5 0.0483856616321674 1108 6 0.04994282539372108 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8025.0 8 8306.0 9 8645.0 10 8602.0 11 8691.0 12 9179.0 13 9473.0 14 9873.0 15 10886.0 16 12467.0 17 14708.0 18 13214.0 19 10911.0 20 11336.0 21 12006.0 22 12896.0 23 14627.0 24 16912.0 25 19455.0 26 22474.0 27 25876.0 28 30545.0 29 36819.0 30 45304.0 31 56166.0 32 71875.0 33 94583.0 34 128169.0 35 184021.0 36 281998.0 37 493667.0 38 487294.0 39 320.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.14787930357946 22.709709639165347 12.815533473758943 23.32687758349625 2 14.605771293013778 21.51586168073215 42.72380389732596 21.154563128928118 3 17.482973192130604 30.09938539922949 30.025996986159303 22.391644422480606 4 11.306897079755968 17.928996759924686 42.5920689499163 28.172037210403044 5 11.982737994248899 38.77660754603709 36.35918718922517 12.881467270488844 6 26.309809059951313 40.77678476655826 20.434047988195232 12.479358185295197 7 24.31179450236043 32.72208330505688 26.703243353292162 16.262878839290533 8 23.452625186407115 37.06465129471378 22.028361722897667 17.454361795981438 9 25.797526636423907 15.652585860378679 23.44901450016792 35.1008730030295 10-11 23.277734541362168 27.261780521513828 30.40425347724462 19.056231459879385 12-13 23.60604611848885 25.329236000210553 30.10822057909342 20.956497302207175 14-15 21.250254225029664 26.82691272386757 27.40455549501284 24.518277556089927 16-17 20.049068507536905 29.10577332603041 29.027683095488037 21.817475070944646 18-19 19.76577024549946 29.647654776812644 30.38833171172265 20.198243265965253 20-21 20.74572432665581 28.381522591916454 29.598933150739875 21.27381993068786 22-23 20.951637685471816 28.919552170500957 28.921488020352086 21.20732212367514 24-25 20.75187538173219 28.854124795756963 29.56375514849056 20.83024467402029 26-27 20.81373557248083 28.62373691234994 29.075137945615236 21.487389569553997 28-29 20.42001851455498 29.473487992510655 28.860650132333827 21.24584336060054 30-31 21.44882483038475 28.865631139254162 28.725706171924347 20.95983785843674 32-33 21.43101066152992 29.06687251928454 28.441984537562448 21.060132281623087 34-35 21.561082370628675 28.317133097725183 29.23070196960759 20.891082562038548 36-37 21.62046293347811 28.58634673376453 29.07252781098449 20.720662521772873 38-39 21.96991645829092 28.249160841716215 29.582678624563457 20.19824407542941 40-41 21.249245233442554 28.112552877161416 29.724512381651735 20.913689507744294 42-43 21.648130360563997 28.22205894380032 28.877333242848668 21.252477452787012 44-45 21.20403172463266 29.212080557058208 28.8064415020804 20.777446216228736 46-47 21.826205410579657 28.468241344654793 28.24251928608715 21.4630339586784 48-49 21.887731559944804 27.81122684400552 28.92151181226341 21.379529783786268 50-51 21.70865949119374 28.2005259295499 28.143739516354486 21.94707506290187 52-53 20.871945999815786 28.6270296886643 28.599964990503345 21.901059321016568 54-55 21.287734069134814 28.872838759563713 28.212127673458486 21.62729949784299 56-57 21.80964723387637 28.12963484157269 28.467172394834506 21.593545529716437 58-59 21.337257009283604 27.882993120694703 29.37749825077447 21.40225161924722 60-61 21.3495140188669 29.311573442032458 28.087137864387696 21.25177467471295 62-63 21.71043031974792 28.63515026413921 27.581188917603967 22.07323049850891 64-65 20.731661835405312 28.570606025776275 28.14714456859864 22.550587570219772 66-67 21.398301342391793 27.39149179576768 28.723285243154635 22.486921618685887 68-69 20.718053706859056 28.225175049301605 28.190639400073913 22.866131843765434 70-71 20.887043948577364 27.9004168342057 28.807918716021558 22.404620501195378 72-73 21.608185678790537 27.71733230282748 28.950364431055277 21.72411758732671 74-75 21.46512237748855 28.51981951264214 28.059721535415427 21.95533657445388 76-77 21.112013767072295 28.30117594107357 28.754087400593825 21.83272289126031 78-79 20.845576068534285 28.212413675073368 28.497922264125243 22.444087992267104 80-81 20.755367837138223 28.378102401279097 29.165168449109185 21.70136131247349 82-83 21.663601000326345 27.378196480708116 29.430178721948717 21.52802379701682 84-85 22.174293147945534 27.621433893649805 28.375836941101333 21.828436017303325 86-87 21.00923951050285 28.582515473143577 28.529373732105 21.878871284248564 88-89 21.701631945625653 27.563800621585578 28.638731079191537 22.09583635359723 90-91 21.537902209908122 27.16227627864453 29.179585131886114 22.120236379561227 92-93 21.518861787767175 27.768687391874547 28.635456777721974 22.076994042636308 94-95 20.50920521212816 27.575952452504044 29.202753947200105 22.712088388167693 96-97 20.993634741186586 27.612885265235093 28.39636038156264 22.99711961201568 98-99 20.368174100282467 28.28358394291931 28.556378115990462 22.79186384080776 100-101 20.836737997049134 27.267473358665267 28.44019529826379 23.45559334602181 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 42.0 1 34.0 2 36.5 3 49.0 4 35.5 5 37.5 6 71.0 7 110.5 8 161.0 9 183.5 10 183.5 11 200.5 12 257.0 13 352.5 14 429.0 15 557.5 16 722.0 17 873.5 18 1023.0 19 1155.0 20 1337.5 21 1476.0 22 1636.0 23 1918.0 24 2258.0 25 2696.0 26 3547.0 27 5219.5 28 8583.5 29 16540.0 30 28489.5 31 42092.0 32 58192.5 33 77270.5 34 101028.5 35 118026.5 36 128243.0 37 141461.0 38 138536.5 39 116261.0 40 94483.0 41 77506.5 42 67137.5 43 62776.5 44 60206.5 45 61464.5 46 63566.0 47 65363.0 48 67194.0 49 65780.0 50 62393.0 51 61018.5 52 59485.5 53 57055.5 54 54812.5 55 49402.0 56 44002.0 57 37593.5 58 31107.5 59 28623.0 60 24885.5 61 19721.0 62 15760.5 63 13138.5 64 10522.5 65 7624.0 66 5774.5 67 4579.5 68 3817.0 69 3270.5 70 2721.5 71 2274.0 72 1993.0 73 1700.5 74 1409.0 75 1241.5 76 1094.5 77 918.0 78 744.5 79 619.5 80 514.0 81 420.5 82 358.5 83 292.5 84 214.5 85 174.0 86 145.0 87 109.0 88 94.0 89 83.5 90 64.0 91 43.5 92 33.5 93 32.0 94 21.5 95 17.5 96 14.0 97 6.5 98 4.5 99 2.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.6101346307442537E-4 2 0.0034366772638132676 3 1.7400897538295026E-4 4 8.700448769147513E-4 5 0.0018270942415209777 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.3502243845737565E-5 12-13 4.3502243845737565E-5 14-15 0.003458428385736136 16-17 0.004741744579185394 18-19 0.0039804553118849865 20-21 2.1751121922868783E-5 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.480179507659005E-4 42-43 0.0 44-45 5.872802919174571E-4 46-47 0.7467160156120853 48-49 0.5366871823248643 50-51 0.4111832088299115 52-53 0.3447552824774702 54-55 0.33564156239178816 56-57 0.30395017775016836 58-59 0.304667964773623 60-61 0.27887113417310067 62-63 0.2734333536923835 64-65 0.26960515623395853 66-67 0.257598536932535 68-69 0.23706547783734683 70-71 0.2494418662114592 72-73 0.824171760779421 74-75 0.5476279966520673 76-77 0.6408533052134828 78-79 0.3592415296781008 80-81 0.30973597618165144 82-83 0.2905949888895269 84-85 0.28085048626808173 86-87 0.25263928113412093 88-89 0.2560542072760113 90-91 0.2674517951635945 92-93 0.22186144361326154 94-95 0.18934351633857274 96-97 0.14710283756436157 98-99 0.15639056662542655 100-101 0.15110504399816943 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2298732.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.1897957442396 #Duplication Level Percentage of deduplicated Percentage of total 1 85.28669372897224 39.393749630427486 2 8.717628249156823 8.053309364055334 3 2.378259514444989 3.2955396359702545 4 1.0185854793287459 1.8819302095297266 5 0.5118701755117018 1.182158942722679 6 0.3280405299569473 0.9091275044726106 7 0.2307176369057454 0.7459760366289011 8 0.16176251454447227 0.5977422004707009 9 0.1304489107355792 0.5422867687741453 >10 0.9076817662747289 8.51161338605881 >50 0.14945594840528106 4.894465898827635 >100 0.1513338369783874 14.32492137655538 >500 0.01730746634901417 5.421740445280797 >1k 0.009457631884707196 7.98393010289865 >5k 7.566105507765757E-4 2.2615084973269215 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 9069 0.3945218494369939 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 7961 0.3463213632559167 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 6351 0.27628275066427926 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 6101 0.2654071897028448 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 5998 0.2609264585867339 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 5808 0.2526610322560438 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 5272 0.22934382955472843 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 5209 0.22660318819244696 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 4788 0.20828874353339147 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 4656 0.20254644734575408 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 4503 0.19589060403735625 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 4389 0.19093134823894217 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 4165 0.18118684561749696 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 3864 0.16809267021992993 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 3491 0.15186633326546983 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 3332 0.14494947649399756 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 3085 0.1342044222641004 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 3048 0.1325948392418081 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 3033 0.131942305584122 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 2982 0.12972369114798943 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 2832 0.1231983545711288 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2733 0.11889163243040077 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 2700 0.11745605838349144 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 2536 0.11032169039279045 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 2483 0.10801607146896637 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 2370 0.10310031791439803 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 2361 0.1027087977197864 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 2305 0.10027267206442508 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.1751121922868783E-5 8.700448769147513E-5 14-15 0.0 0.0 0.0 4.3502243845737565E-5 8.700448769147513E-5 16-17 0.0 0.0 0.0 4.3502243845737565E-5 8.700448769147513E-5 18-19 2.1751121922868783E-5 0.0 0.0 4.3502243845737565E-5 8.700448769147513E-5 20-21 4.3502243845737565E-5 0.0 0.0 4.3502243845737565E-5 2.1751121922868781E-4 22-23 4.3502243845737565E-5 0.0 0.0 6.525336576860636E-5 2.1751121922868781E-4 24-25 4.3502243845737565E-5 0.0 0.0 2.1751121922868781E-4 2.1751121922868781E-4 26-27 4.3502243845737565E-5 0.0 0.0 4.132713165345068E-4 2.6101346307442537E-4 28-29 4.3502243845737565E-5 0.0 0.0 0.0013485695592178643 2.6101346307442537E-4 30-31 4.3502243845737565E-5 0.0 0.0 0.005959807406866046 2.6101346307442537E-4 32-33 4.3502243845737565E-5 0.0 0.0 0.021098588265182718 2.6101346307442537E-4 34-35 4.3502243845737565E-5 0.0 0.0 0.047134681206856655 3.045157069201629E-4 36-37 4.3502243845737565E-5 0.0 0.0 0.08976688017567946 3.045157069201629E-4 38-39 4.3502243845737565E-5 0.0 0.0 0.14914744302511124 3.697690726887693E-4 40-41 4.3502243845737565E-5 0.0 0.0 0.21390053298949158 3.915201946116381E-4 42-43 4.3502243845737565E-5 0.0 0.0 0.2825253226561426 3.915201946116381E-4 44-45 4.3502243845737565E-5 0.0 0.0 0.3688337744460859 3.915201946116381E-4 46-47 4.3502243845737565E-5 0.0 0.0 0.4778503975235043 3.915201946116381E-4 48-49 4.3502243845737565E-5 0.0 0.0 0.5938491307381635 3.915201946116381E-4 50-51 4.3502243845737565E-5 0.0 0.0 0.7127407631685643 3.915201946116381E-4 52-53 4.3502243845737565E-5 0.0 0.0 0.83295921403626 3.915201946116381E-4 54-55 4.3502243845737565E-5 0.0 0.0 0.9670766318126689 3.915201946116381E-4 56-57 8.700448769147513E-5 0.0 0.0 1.132646171889546 3.915201946116381E-4 58-59 8.700448769147513E-5 0.0 0.0 1.3188575266712257 3.915201946116381E-4 60-61 8.700448769147513E-5 0.0 0.0 1.5143348593920476 3.915201946116381E-4 62-63 8.700448769147513E-5 0.0 0.0 1.7154457326908923 4.785246823031132E-4 64-65 8.700448769147513E-5 0.0 0.0 1.9265186198304107 4.785246823031132E-4 66-67 8.700448769147513E-5 0.0 0.0 2.167107779419262 4.785246823031132E-4 68-69 8.700448769147513E-5 0.0 0.0 2.438409523163205 4.785246823031132E-4 70-71 8.700448769147513E-5 0.0 0.0 2.717041394995154 5.002758042259819E-4 72-73 8.700448769147513E-5 0.0 0.0 3.02438474776529 5.220269261488507E-4 74-75 8.700448769147513E-5 0.0 0.0 3.324202212350113 5.220269261488507E-4 76-77 8.700448769147513E-5 0.0 0.0 3.6642375013703203 5.220269261488507E-4 78-79 8.700448769147513E-5 0.0 0.0 4.041989235804783 5.220269261488507E-4 80-81 8.700448769147513E-5 0.0 0.0 4.468833252419159 5.220269261488507E-4 82-83 8.700448769147513E-5 0.0 0.0 4.919777512124075 5.655291699945883E-4 84-85 8.700448769147513E-5 0.0 0.0 5.3673720990528695 5.655291699945883E-4 86-87 8.700448769147513E-5 0.0 0.0 5.844243696089844 6.090314138403258E-4 88-89 8.700448769147513E-5 0.0 0.0 6.358092200395697 6.525336576860634E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1765 0.0 50.429634 2 ACCTGGG 1860 0.0 46.5802 3 GTACCTG 2170 0.0 41.23583 1 CCTGGGG 1955 0.0 38.02035 4 TATCACG 175 3.6379788E-12 32.465034 2 CTGGGGG 1795 0.0 32.44172 5 GTACATG 7200 0.0 28.012365 1 TGGGGGG 5095 0.0 27.504345 6 ACATGGG 7170 0.0 26.94041 3 CATGGGG 5270 0.0 26.8611 4 TACATGG 7410 0.0 26.707346 2 GTATCAA 6025 0.0 26.088776 1 ATGGGGG 4635 0.0 25.63818 5 GTACACG 225 1.07320375E-10 25.250582 1 CTGTGCG 1575 0.0 25.250582 9 GTGATCG 755 0.0 25.082268 8 CAACGCA 6005 0.0 25.071327 5 GTACTAG 1195 0.0 24.56385 1 ATCAACG 6180 0.0 24.208162 3 TAGTACT 1725 0.0 24.152205 4 >>END_MODULE