FastQCFastQC Report
Sat 18 Jun 2016
SRR1931086_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931086_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646159
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64390.391152980969639No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA42960.2609711455576284No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC41540.25234500434040696No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA40130.24377961059654624No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT38460.233634782545307No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT37590.22834975236292482No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC36340.22075631819283556No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC35010.21267690423586058No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC34350.20866757099405345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30470.1850975513300963No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27800.16887797594278559No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT25760.1564854913771999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25690.15606025906367488No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT24270.14743411784645347No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC23010.13977993620300347No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA22020.13376593634029277No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT20030.12167718914151064No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA19090.11596692664560349No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT18950.1151164620185535No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG18640.11323329034437135No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC18090.10989217930953206No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC17990.10928470457592493No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC17490.10624733090788922No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT17330.10527537133411778No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT16750.10175201787919635No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG16720.10156977545911422No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC16620.10096230072550708No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT16560.10059781588534278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG14650.051.415822
ACCTGGG15400.048.601233
GTACCTG15950.047.8192181
CCTGGGG16650.039.831324
GTACACG2500.039.7939071
CTGGGGG16350.036.5059555
GTACTAG14050.032.0320361
TAGGCAT17050.029.7283155
GCATCGT15250.029.1990628
TATTCTC9250.027.6543165
TAGTACT20150.027.0354564
CTGTGCG20050.026.6985789
TGGGGGG54100.026.356046
CTAGGCA20150.026.3301834
TATCACG1109.652198E-525.8401992
ACTGTGC21800.025.4237888
GTACTGT21650.024.7247246
TGAACCG2900.024.5021465
AGGGCTA18250.024.3992185
GTACATG82200.024.2631721
Produced by FastQC (version 0.11.5)