##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931086_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1646159 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.616123351389504 33.0 33.0 33.0 33.0 33.0 2 32.57583684200615 33.0 33.0 33.0 33.0 33.0 3 32.62456542776245 33.0 33.0 33.0 33.0 33.0 4 36.56519449214809 37.0 37.0 37.0 37.0 37.0 5 36.51339572908814 37.0 37.0 37.0 37.0 37.0 6 36.518706880684064 37.0 37.0 37.0 37.0 37.0 7 36.4514205492908 37.0 37.0 37.0 37.0 37.0 8 36.464265602532926 37.0 37.0 37.0 37.0 37.0 9 36.44754000069252 37.0 37.0 37.0 37.0 37.0 10-11 36.42396937355383 37.0 37.0 37.0 37.0 37.0 12-13 36.33517691790404 37.0 37.0 37.0 37.0 37.0 14-15 38.19144292865999 40.0 37.0 40.0 35.0 40.0 16-17 38.1893319539607 40.0 37.0 40.0 35.0 40.0 18-19 38.1508684762529 40.0 37.0 40.0 35.0 40.0 20-21 38.05172556235455 40.0 37.0 40.0 33.0 40.0 22-23 37.89884057372343 40.0 37.0 40.0 33.0 40.0 24-25 38.06298358785512 40.0 37.0 40.0 33.0 40.0 26-27 37.9841628299575 40.0 37.0 40.0 33.0 40.0 28-29 37.892332393164935 40.0 37.0 40.0 33.0 40.0 30-31 37.750008352777584 40.0 37.0 40.0 33.0 40.0 32-33 37.601860755856514 40.0 37.0 40.0 33.0 40.0 34-35 37.4889810158071 40.0 37.0 40.0 33.0 40.0 36-37 37.409673670647855 40.0 37.0 40.0 33.0 40.0 38-39 37.225650438384136 40.0 37.0 40.0 33.0 40.0 40-41 37.021007387500234 40.0 37.0 40.0 33.0 40.0 42-43 36.79336412825249 40.0 37.0 40.0 33.0 40.0 44-45 36.49195521210284 37.0 37.0 40.0 33.0 40.0 46-47 36.16461836311073 37.0 37.0 40.0 33.0 40.0 48-49 35.82967441176703 37.0 37.0 40.0 33.0 40.0 50-51 35.50549187532918 37.0 37.0 40.0 27.0 40.0 52-53 35.25034580499211 37.0 37.0 40.0 27.0 40.0 54-55 34.97520379258626 37.0 33.0 40.0 27.0 40.0 56-57 34.60302224754717 37.0 33.0 40.0 27.0 40.0 58-59 34.272807790741965 37.0 33.0 40.0 27.0 40.0 60-61 33.975319516523015 37.0 33.0 38.5 27.0 40.0 62-63 33.63667938516267 37.0 33.0 37.0 27.0 40.0 64-65 33.252758694634 37.0 33.0 37.0 24.5 40.0 66-67 32.85766715122901 37.0 33.0 37.0 22.0 40.0 68-69 32.51212853679384 37.0 33.0 37.0 22.0 40.0 70-71 32.21726273099986 37.0 33.0 37.0 22.0 40.0 72-73 31.868317701995977 37.0 33.0 37.0 22.0 37.0 74-75 31.472729244258907 33.0 33.0 37.0 18.5 37.0 76-77 29.83599063030971 33.0 27.0 37.0 15.0 37.0 78-79 30.654762996770057 33.0 33.0 37.0 15.0 37.0 80-81 30.714122086627114 33.0 33.0 37.0 15.0 37.0 82-83 30.474987531581093 33.0 33.0 37.0 15.0 37.0 84-85 30.165396234507117 33.0 27.0 37.0 15.0 37.0 86-87 29.8258327415517 33.0 27.0 37.0 6.0 37.0 88-89 29.468554677889557 33.0 27.0 37.0 4.0 37.0 90-91 29.004809681203334 33.0 27.0 37.0 2.0 37.0 92-93 28.568224879856686 33.0 27.0 37.0 2.0 37.0 94-95 28.03607033099476 33.0 27.0 37.0 2.0 37.0 96-97 27.482356200099748 33.0 27.0 35.0 2.0 37.0 98-99 26.767565587528303 33.0 27.0 33.0 2.0 37.0 100-101 25.035029726776088 33.0 18.5 33.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.010325861500653843 1101 2 0.15284946737195781 1101 3 0.0610880540048484 1101 4 0.07931676161908996 1101 5 0.06488232230057633 1101 6 0.04290567329410777 1101 7 0.08416812303633492 1101 8 0.18923823014627317 1101 9 0.11881616895175995 1101 10-11 0.1063413956617083 1101 12-13 0.10802251351982761 1101 14-15 0.2894788875401133 1101 16-17 0.20123854961937582 1101 18-19 0.12438193137172249 1101 20-21 0.07648676012032496 1101 22-23 0.062342440723377024 1101 24-25 0.06594057407495768 1101 26-27 0.17178910486445176 1101 28-29 0.123695814082744 1101 30-31 0.006986072252523456 1101 32-33 -0.03320394801509963 1101 34-35 -0.127847875853206 1101 36-37 -0.08228206242966962 1101 38-39 -0.14104563350932153 1101 40-41 -0.2197012231224562 1101 42-43 -0.42592952845683385 1101 44-45 -0.6741628595639071 1101 46-47 -0.6709171210825389 1101 48-49 -0.7055500665249923 1101 50-51 -0.6483853739307008 1101 52-53 -0.6896567890062428 1101 54-55 -0.6309018675395208 1101 56-57 -0.782041918654194 1101 58-59 -0.6473284453075365 1101 60-61 -0.7609044052254887 1101 62-63 -0.9759523726807657 1101 64-65 -1.1038391878862797 1101 66-67 -1.110519787223616 1101 68-69 -1.2931512570938182 1101 70-71 -1.412092910195394 1101 72-73 -1.596288410097106 1101 74-75 -1.6090528647361957 1101 76-77 -1.4795514287115985 1101 78-79 -1.5334263038744531 1101 80-81 -1.679875785125727 1101 82-83 -1.9252776031646626 1101 84-85 -1.8613189457343822 1101 86-87 -2.1117662237841657 1101 88-89 -2.2861689228648174 1101 90-91 -2.309379628309191 1101 92-93 -2.428517840470999 1101 94-95 -2.5196812345435085 1101 96-97 -2.551686603141466 1101 98-99 -3.085105791062624 1101 100-101 -4.241014610736208 1102 1 -0.001605110765211748 1102 2 0.18899896003240002 1102 3 0.1303181679754033 1102 4 0.14579710556344594 1102 5 0.11485142626136025 1102 6 0.1616825777498505 1102 7 0.14013434877528397 1102 8 0.22751326213527534 1102 9 0.13011406851686758 1102 10-11 0.11961317128480431 1102 12-13 0.20526458567728412 1102 14-15 0.3033525838106428 1102 16-17 0.18637059131347655 1102 18-19 0.25470642767163554 1102 20-21 0.17247608915570112 1102 22-23 0.2021712437644041 1102 24-25 0.30756765730406954 1102 26-27 0.2819112873191969 1102 28-29 0.3710261574186404 1102 30-31 0.24879772044241122 1102 32-33 0.24124910645612374 1102 34-35 0.2496036944363027 1102 36-37 0.15566070755912165 1102 38-39 0.27442158626629976 1102 40-41 0.12335726391360424 1102 42-43 0.29153757323327767 1102 44-45 0.24093230068760363 1102 46-47 0.29679820808534885 1102 48-49 -0.022389061427929846 1102 50-51 -0.11452074590685157 1102 52-53 -0.07389354416042693 1102 54-55 0.07282652495150188 1102 56-57 0.1411635082317133 1102 58-59 0.15427932057305327 1102 60-61 0.002751937071074906 1102 62-63 0.1074320033431917 1102 64-65 -0.06258227120733295 1102 66-67 -0.17831710019215308 1102 68-69 -0.26564544408629587 1102 70-71 -0.36690559366175535 1102 72-73 -0.4865029065818085 1102 74-75 -0.4650592936363189 1102 76-77 -0.3893595577727602 1102 78-79 -0.43665454560361994 1102 80-81 -0.577420597903842 1102 82-83 -0.8128097104818686 1102 84-85 -0.9293163730926324 1102 86-87 -1.084842274914294 1102 88-89 -1.0226987230073306 1102 90-91 -1.132254956593691 1102 92-93 -1.200407943417229 1102 94-95 -1.0923998290914732 1102 96-97 -1.3684291465142309 1102 98-99 -1.6932155619181266 1102 100-101 -2.2489644252923675 1103 1 0.13867991745694752 1103 2 0.21294756763523282 1103 3 0.09184871339592604 1103 4 0.17269204428549756 1103 5 0.05596721507851754 1103 6 0.15828469207298923 1103 7 0.1833201784444185 1103 8 0.07895430744665788 1103 9 0.1523874336586033 1103 10-11 0.15692442584556687 1103 12-13 0.20976952845335006 1103 14-15 0.2886259177676678 1103 16-17 0.2664653928228162 1103 18-19 0.20697885727215493 1103 20-21 0.14981353887036875 1103 22-23 0.14563087516116013 1103 24-25 0.306246673114714 1103 26-27 0.2543296613485708 1103 28-29 0.28073707518456814 1103 30-31 0.2398651729636967 1103 32-33 0.2098330089103655 1103 34-35 0.12896004208341338 1103 36-37 0.14314316616142264 1103 38-39 0.2740985127015847 1103 40-41 0.17163929840388903 1103 42-43 0.11966306049432518 1103 44-45 0.08924115402592747 1103 46-47 0.18105679167595667 1103 48-49 0.1341307252686761 1103 50-51 0.13436689559376447 1103 52-53 0.05291719585267174 1103 54-55 0.1900280093435569 1103 56-57 0.21984252404236315 1103 58-59 0.22530748036349735 1103 60-61 0.13016890778863655 1103 62-63 0.01035401039092676 1103 64-65 0.13734413288410252 1103 66-67 -0.017660350262318047 1103 68-69 -0.010121073237762346 1103 70-71 0.0943860769029996 1103 72-73 0.14416101583064034 1103 74-75 -0.09262096139444864 1103 76-77 -0.20675688212489973 1103 78-79 -0.05720152536474643 1103 80-81 -0.11040528621269274 1103 82-83 -0.19402840883219596 1103 84-85 -0.33639842005680265 1103 86-87 -0.28551336980935815 1103 88-89 -0.2860666713654574 1103 90-91 0.09248449517916058 1103 92-93 0.03282497515950311 1103 94-95 -0.23947563911423586 1103 96-97 -0.5607920117651126 1103 98-99 -0.6429196190485094 1103 100-101 -1.6140905674322603 1104 1 0.0920799274086832 1104 2 0.2223439949626922 1104 3 0.16553135259580642 1104 4 0.13948111728146273 1104 5 0.1195209793219405 1104 6 0.14710690434347384 1104 7 0.1595335436232972 1104 8 0.15783374218815993 1104 9 0.11571330319137019 1104 10-11 0.11906504384824501 1104 12-13 0.20532309366208779 1104 14-15 0.2649875637844872 1104 16-17 0.21388042989302392 1104 18-19 0.1750875302084154 1104 20-21 0.13324987265152544 1104 22-23 0.17391270827058491 1104 24-25 0.23701544457313872 1104 26-27 0.20557910156352932 1104 28-29 0.2820507040844049 1104 30-31 0.21054437881531385 1104 32-33 0.2970659292467346 1104 34-35 0.24901676696772768 1104 36-37 0.3122878146164112 1104 38-39 0.32135730754657743 1104 40-41 0.3061290232384408 1104 42-43 0.26810810204280955 1104 44-45 0.30370500435185477 1104 46-47 0.25761448685433663 1104 48-49 0.2914467425846965 1104 50-51 0.28897803158420743 1104 52-53 0.28840308926960034 1104 54-55 0.410369353822837 1104 56-57 0.40875131639943163 1104 58-59 0.22210998820048644 1104 60-61 0.2841829397337037 1104 62-63 0.14890205238724974 1104 64-65 0.14436806680458858 1104 66-67 0.21755983185441607 1104 68-69 0.08128062850877171 1104 70-71 0.0732764585520087 1104 72-73 0.07904184381491319 1104 74-75 0.02739297103327587 1104 76-77 -0.04196213664049964 1104 78-79 -0.05160467011271663 1104 80-81 -0.13918470602659383 1104 82-83 -0.09398163568197404 1104 84-85 0.07517047082243522 1104 86-87 -0.014307717224390615 1104 88-89 0.04594407650735732 1104 90-91 0.11201875402376515 1104 92-93 0.0677117741842288 1104 94-95 -0.03508294368705833 1104 96-97 0.015478078298084341 1104 98-99 -0.20441337433537043 1104 100-101 -0.9041239057414643 1105 1 0.07519831330420601 1105 2 0.20547921197896102 1105 3 0.09592204498603962 1105 4 0.05987412395079872 1105 5 0.18029815632221613 1105 6 0.15630090404893338 1105 7 0.14036289709787297 1105 8 0.11141351118102705 1105 9 0.09809532755441808 1105 10-11 0.11510973047382578 1105 12-13 0.1365574459795198 1105 14-15 0.21287841396647877 1105 16-17 0.28013613669490667 1105 18-19 0.18887432198641108 1105 20-21 0.11403504443415358 1105 22-23 0.13010340486859207 1105 24-25 0.029946373861399422 1105 26-27 0.18152743002284666 1105 28-29 0.2419968444980256 1105 30-31 0.27656234603670526 1105 32-33 0.10399824710245298 1105 34-35 0.1279652426993536 1105 36-37 0.22368879502919725 1105 38-39 0.34762437535495394 1105 40-41 0.39716434755310104 1105 42-43 0.356736576091329 1105 44-45 0.4060950234972509 1105 46-47 0.43813456848527466 1105 48-49 0.4482496711997044 1105 50-51 0.509129090892877 1105 52-53 0.41229486516322567 1105 54-55 0.4329335750679135 1105 56-57 0.38652371756919024 1105 58-59 0.5558626969589824 1105 60-61 0.4029496393613172 1105 62-63 0.2982424828431647 1105 64-65 0.43603245425698844 1105 66-67 0.6484554578669091 1105 68-69 0.35376805776547116 1105 70-71 0.23828876630904716 1105 72-73 0.1164195552032723 1105 74-75 0.41741632421330266 1105 76-77 0.3021585424952242 1105 78-79 0.4782263875128301 1105 80-81 0.5229137139519437 1105 82-83 0.46969259797606 1105 84-85 0.5283652279283224 1105 86-87 0.5198152761904282 1105 88-89 0.5786574635600203 1105 90-91 0.44202864230719996 1105 92-93 0.6532412181260803 1105 94-95 0.5702988071702748 1105 96-97 0.35969764026816264 1105 98-99 0.5564311271478779 1105 100-101 -0.3244201334665249 1106 1 0.09250560180105794 1106 2 0.2011945957823258 1106 3 0.13006292475192538 1106 4 0.1642830142150018 1106 5 0.19393019456554583 1106 6 0.15829896886427974 1106 7 0.20466834377759113 1106 8 0.13982657973679125 1106 9 0.245926590733049 1106 10-11 0.1669485179308694 1106 12-13 0.21138121955910805 1106 14-15 0.266911909657658 1106 16-17 0.16194639180163506 1106 18-19 0.08503840338917001 1106 20-21 0.09531962137832295 1106 22-23 0.09328383015952824 1106 24-25 0.2001873649442274 1106 26-27 0.22351499194551394 1106 28-29 0.3603272123012502 1106 30-31 0.2352763742961983 1106 32-33 0.1377389803766107 1106 34-35 0.21864625010816496 1106 36-37 0.1717324407219678 1106 38-39 0.2545426795417498 1106 40-41 0.237950631598423 1106 42-43 0.3842794213005689 1106 44-45 0.4315214748571563 1106 46-47 0.36837245748006353 1106 48-49 0.41492070772012113 1106 50-51 0.29097913199363035 1106 52-53 0.2844474894191933 1106 54-55 0.5166224988986983 1106 56-57 0.48589647841611594 1106 58-59 0.6644205347045329 1106 60-61 0.43191662498919925 1106 62-63 0.34706816721751466 1106 64-65 0.6160225382458364 1106 66-67 0.4547188175197263 1106 68-69 0.47043572162260006 1106 70-71 0.6294449190948868 1106 72-73 0.6515472724361331 1106 74-75 0.5391397437204084 1106 76-77 0.6175416217863479 1106 78-79 0.7433013349507327 1106 80-81 0.552727676320643 1106 82-83 0.5909478460878468 1106 84-85 0.6454492346827934 1106 86-87 0.6578938260900316 1106 88-89 0.49124393807506905 1106 90-91 0.610007471890146 1106 92-93 0.7583326439775995 1106 94-95 0.7931965994668069 1106 96-97 0.9203110686509 1106 98-99 1.074689811565296 1106 100-101 0.843758197724437 1107 1 -0.026858164906158777 1107 2 0.20431728199451982 1107 3 0.07545518031641762 1107 4 0.08518666438150291 1107 5 0.08793727154451858 1107 6 0.01784105691324811 1107 7 0.17364775482065653 1107 8 0.10939224082780896 1107 9 0.16725472936020225 1107 10-11 0.05568622025045755 1107 12-13 0.09607017772039228 1107 14-15 0.16635826524509412 1107 16-17 0.0940776350932282 1107 18-19 0.10333266182990286 1107 20-21 0.1678530466781254 1107 22-23 0.18473974234650825 1107 24-25 0.28983603469944086 1107 26-27 0.32635084989351526 1107 28-29 0.3485349011515737 1107 30-31 0.3047473668609513 1107 32-33 0.3110384752606734 1107 34-35 0.43184422240675246 1107 36-37 0.5206386139349632 1107 38-39 0.3971009589485277 1107 40-41 0.44806391537311896 1107 42-43 0.3340821228276454 1107 44-45 0.4689788722109256 1107 46-47 0.4163997260687182 1107 48-49 0.24314799613993898 1107 50-51 0.3099989653393891 1107 52-53 0.25203654695348376 1107 54-55 0.38942288509996104 1107 56-57 0.4111877759322269 1107 58-59 0.5636736449057906 1107 60-61 0.5430023238897306 1107 62-63 0.4725383433748007 1107 64-65 0.5667380889987967 1107 66-67 0.7617459290512585 1107 68-69 0.7953002146284902 1107 70-71 0.7909056008964583 1107 72-73 0.7969962712151428 1107 74-75 0.7494055610723969 1107 76-77 0.5419392498620788 1107 78-79 0.667537020497786 1107 80-81 0.6879180230260253 1107 82-83 0.7984908576615801 1107 84-85 0.8234454247812479 1107 86-87 0.8089833102840771 1107 88-89 0.7416456279227397 1107 90-91 0.9432443031579716 1107 92-93 1.0069359575207706 1107 94-95 1.1596251352240792 1107 96-97 1.3879701504964714 1107 98-99 1.6652361314710866 1107 100-101 2.0650786282491005 1108 1 0.06763879477082924 1108 2 0.011521239425412944 1108 3 0.125529296991715 1108 4 0.05385492888535026 1108 5 0.05889093666815626 1108 6 0.05023656157149503 1108 7 0.07053616451851497 1108 8 0.023263620393620954 1108 9 0.057868604080418606 1108 10-11 0.045114686687036 1108 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0.6501227641323517 2316 94-95 0.761723433293902 2316 96-97 1.0153244969710435 2316 98-99 1.202764479947934 2316 100-101 1.517841382509765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 17.0 5 28.0 6 63.0 7 165.0 8 469.0 9 930.0 10 1566.0 11 2376.0 12 3524.0 13 5220.0 14 5977.0 15 7793.0 16 10092.0 17 13261.0 18 13173.0 19 10402.0 20 9195.0 21 9271.0 22 9647.0 23 11069.0 24 12486.0 25 14762.0 26 17954.0 27 21708.0 28 26385.0 29 33294.0 30 41169.0 31 52375.0 32 68476.0 33 91864.0 34 130330.0 35 199388.0 36 304187.0 37 382310.0 38 135229.0 39 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.21449306199371 22.58564980166269 13.742571849780386 24.45728528656322 2 14.258048133897233 21.675379884555433 39.65075041399429 24.415821567553053 3 17.809215270213873 28.032043077248307 29.670463181260132 24.488278471277685 4 11.924789768181567 17.613000931258764 38.92114917210306 31.541060128456603 5 12.53493739061658 38.70944422744097 34.76243789330192 13.993180488640528 6 29.011292347823026 38.65847709729133 18.313115561740997 14.017114993144647 7 26.32431010613191 31.939381311282812 24.030667754451425 17.705640828133856 8 26.36379596381638 34.059346636625015 19.98458229126105 19.592275108297557 9 26.27808127890441 15.146592765340408 22.3752383578986 36.20008759785659 10-11 25.77038427029224 25.90664085304032 27.546245532782677 20.776729343884764 12-13 26.201782452363346 23.985562755481094 27.702852519106596 22.10980227304896 14-15 23.142913724961332 25.03070794105533 26.25121950923182 25.575158824751508 16-17 21.72636422119613 27.161531783989272 27.433680464645278 23.678423530169322 18-19 21.257393726851415 27.924398554453123 29.23217016096258 21.58603755773288 20-21 22.720253632850778 26.675977229417086 28.23858448667474 22.3651846510574 22-23 22.965430435334618 26.483255870180216 27.947239604436753 22.60407409004841 24-25 22.756246510817 26.547405202049134 28.766814141282826 21.929534145851036 26-27 22.601067090117056 26.30110457130812 28.480906157910628 22.6169221806642 28-29 21.59615201204744 27.51332040222117 28.528653671972148 22.361873913759243 30-31 22.754059601776014 26.699820612711168 28.183638397019973 22.36248138849285 32-33 23.14746023926 27.058534442906186 27.518939543507038 22.275065774326784 34-35 23.113927633964884 26.526599192423088 28.54080316664429 21.818670006967736 36-37 22.901038113572262 26.058813273808912 28.536793833402484 22.503354779216345 38-39 22.75959453479115 26.464857424118033 28.982871333471234 21.792676707619588 40-41 22.367476644257344 25.85824020246453 29.129000972289003 22.645282180989124 42-43 22.50871543706888 26.246853497125127 27.9658532866242 23.27857777918179 44-45 22.433829295955007 26.88309573984044 28.014669299867144 22.668405664337406 46-47 22.91358246682125 26.6703884618679 27.60684113745999 22.80918793385086 48-49 23.565272460259727 26.363850360612517 27.67317222076259 22.397704958365164 50-51 22.574064838208216 26.54640286873868 27.67390634805022 23.205625945002883 52-53 22.33350484369979 27.278227680315208 26.983693555725786 23.40457392025922 54-55 22.275369511693587 26.89214711337119 27.0871161291224 23.74536724581283 56-57 23.64692596523179 26.244184188769125 26.820039862492017 23.288849983507063 58-59 22.9365450117516 26.648580118931402 27.50627369531133 22.908601174005668 60-61 22.83694952917671 27.512044705280598 26.955749717979856 22.695256047562843 62-63 22.99291866703034 26.715675703258313 26.538809434568595 23.752596195142754 64-65 21.949520064586714 27.38532547585015 26.832158983427483 23.83299547613566 66-67 22.792001936671216 26.358866755744632 26.70650166892095 24.142629638663198 68-69 22.17594306854077 26.53832706732088 26.95933506252394 24.32639480161441 70-71 22.5580046879409 26.32008181571599 26.994660534335356 24.127252962007752 72-73 23.158637774358372 26.102490707155262 27.539897421816484 23.198974096669883 74-75 22.840456383895106 27.411311672391108 26.706367189540735 23.04186475417305 76-77 22.730350194197964 26.68034319292619 26.620171742259647 23.969134870616195 78-79 22.36546545461062 26.9298728155213 26.967659706568387 23.737002023299688 80-81 22.20568138706496 27.172498298244722 27.426797185702277 23.195023128988037 82-83 22.953348265679114 25.87499719031329 28.496389324770018 22.675265219237577 84-85 23.046754214002753 26.855986717849685 26.971561594271133 23.125697473876432 86-87 21.898268265464996 27.423088862335398 27.22948595908157 23.44915691311804 88-89 22.507455233668196 26.3897351349414 26.965864172294413 24.136945459095994 90-91 22.386854724759107 25.959628419629222 27.43685600045682 24.21666085515485 92-93 22.83204785485114 26.627127812898564 27.066153669984622 23.474670662265677 94-95 21.352554435235852 26.619525152518897 27.747762524622484 24.28015788762277 96-97 21.421520206935345 26.86611773882553 27.315751361688527 24.396610692550595 98-99 21.20244763719665 27.64608400525101 26.664981936738798 24.48648642081354 100-101 19.7910465350793 27.7335805355021 26.776654217461772 25.698718711956825 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 61.0 1 48.0 2 57.0 3 71.5 4 52.5 5 56.0 6 98.0 7 183.5 8 282.5 9 377.5 10 424.5 11 441.0 12 539.5 13 662.0 14 802.5 15 1010.0 16 1194.5 17 1356.0 18 1557.5 19 1704.0 20 1871.0 21 2154.0 22 2422.5 23 2656.0 24 2932.0 25 3307.5 26 4037.0 27 4935.5 28 6284.0 29 9675.0 30 14568.0 31 18950.0 32 25078.5 33 31430.0 34 35903.0 35 40883.5 36 44590.5 37 51290.0 38 53645.0 39 49369.5 40 45768.0 41 41653.0 42 40328.5 43 42259.0 44 47099.5 45 56244.5 46 63980.5 47 73306.5 48 87378.0 49 85500.5 50 74305.0 51 71055.0 52 67624.5 53 63656.5 54 61514.0 55 55418.5 56 47963.0 57 39682.5 58 32489.0 59 30769.5 60 26127.5 61 19063.5 62 14070.5 63 11155.5 64 8529.0 65 5229.5 66 3479.0 67 2643.0 68 2167.0 69 1801.5 70 1428.5 71 1093.5 72 857.5 73 694.5 74 562.0 75 466.5 76 358.0 77 284.0 78 227.5 79 182.0 80 156.0 81 126.5 82 110.0 83 91.5 84 77.0 85 60.5 86 51.5 87 44.0 88 31.5 89 21.0 90 16.5 91 11.0 92 7.0 93 4.0 94 2.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07496238212712138 2 6.0747473360714246E-5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.0373736680357124E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.0373736680357123E-5 40-41 0.0026425150911910697 42-43 0.005193908972341068 44-45 0.0 46-47 0.0 48-49 9.112121004107137E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0021565353043053554 68-69 0.006651848332998209 70-71 0.001245323203894642 72-73 0.0 74-75 6.0747473360714246E-5 76-77 0.00179205046414107 78-79 0.005193908972341068 80-81 0.003918212031766069 82-83 0.004130828188528569 84-85 0.00242989893442857 86-87 3.6448484016428546E-4 88-89 0.0 90-91 6.0747473360714246E-5 92-93 8.808383637303565E-4 94-95 0.003128494878076784 96-97 0.002490646407789284 98-99 0.0 100-101 1.8224242008214273E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1646159.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.661328053277156 #Duplication Level Percentage of deduplicated Percentage of total 1 81.7855980786868 25.894406508027767 2 9.758409546471922 6.179284118581582 3 2.748068605362549 2.610225048818865 4 1.2064195215966522 1.5278737697259717 5 0.7494848300930409 1.186484253826523 6 0.47950353855135003 0.9109031302068915 7 0.3498145715603173 0.7752915735591464 8 0.279799827508933 0.7087067302408552 9 0.21154429394772312 0.602799595962998 >10 1.7294686352497373 11.483386949919634 >50 0.3018979184284929 6.783356144681722 >100 0.34408832791774413 23.20844576389624 >500 0.03874608010198505 8.186405303358535 >1k 0.016770691387426363 9.160666388390535 >5k 3.855331353431348E-4 0.7817647208027874 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7144 0.43397994968894255 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 5665 0.34413443658844617 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 4757 0.2889757307769177 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 4416 0.26826084236091413 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4194 0.25477490327483554 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 4081 0.24791043878507482 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 3963 0.24074223692851054 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 3946 0.23970952988137842 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 3645 0.22142454039980344 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3496 0.21237316686905702 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3470 0.21079373256167844 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2682 0.1629247235534356 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 2451 0.1488920572071106 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2430 0.14761636026653563 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 2138 0.12987809804520706 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 2093 0.12714446174397492 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 2065 0.12544353248987491 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 2058 0.1250183001763499 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 2044 0.12416783554929992 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 2016 0.12246690629519992 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1946 0.11821458315994993 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 1939 0.11778935084642492 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 1901 0.11548094685871779 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 1856 0.11274731055748563 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 1854 0.1126258156107642 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 1841 0.11183609845707493 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 1755 0.10661181574805349 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1746 0.10606508848780707 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 1741 0.1057613511210035 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 1736 0.10545761375419993 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 1711 0.10393892692018207 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1699 0.10320995723985352 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 1680 0.10205575524599993 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 1664 0.1010837956722285 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 1655 0.10053706841198207 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.0373736680357123E-5 0.0 16-17 0.0 0.0 0.0 6.0747473360714246E-5 0.0 18-19 0.0 0.0 0.0 6.0747473360714246E-5 0.0 20-21 0.0 0.0 0.0 9.112121004107137E-5 6.0747473360714246E-5 22-23 0.0 0.0 0.0 2.733636301232141E-4 1.2149494672142849E-4 24-25 0.0 0.0 0.0 4.252323135249997E-4 1.8224242008214273E-4 26-27 0.0 0.0 0.0 6.985959436482138E-4 1.8224242008214273E-4 28-29 0.0 0.0 0.0 0.0017920504641410703 1.8224242008214273E-4 30-31 0.0 0.0 0.0 0.006742969543039282 1.8224242008214273E-4 32-33 0.0 0.0 0.0 0.0204718985225607 1.8224242008214273E-4 34-35 0.0 0.0 0.0 0.04364705960967319 1.8224242008214273E-4 36-37 0.0 0.0 0.0 0.0803992809929053 1.8224242008214273E-4 38-39 0.0 0.0 0.0 0.12823791626446776 2.4298989344285698E-4 40-41 0.0 0.0 0.0 0.17683589495303914 2.4298989344285698E-4 42-43 0.0 0.0 0.0 0.2312960048209195 2.4298989344285698E-4 44-45 3.0373736680357123E-5 0.0 0.0 0.2961743063701623 2.4298989344285698E-4 46-47 6.0747473360714246E-5 0.0 0.0 0.37475116316224616 2.4298989344285698E-4 48-49 6.0747473360714246E-5 0.0 0.0 0.45979762586724615 2.4298989344285698E-4 50-51 6.0747473360714246E-5 0.0 0.0 0.5494608965476604 2.4298989344285698E-4 52-53 6.0747473360714246E-5 0.0 0.0 0.6363905309268425 3.6448484016428546E-4 54-55 6.0747473360714246E-5 0.0 0.0 0.7332827509371816 4.8597978688571397E-4 56-57 6.0747473360714246E-5 0.0 0.0 0.8548384451319708 4.8597978688571397E-4 58-59 6.0747473360714246E-5 0.0 0.0 0.9906394218298475 4.8597978688571397E-4 60-61 6.0747473360714246E-5 0.0 0.0 1.131786176183467 4.8597978688571397E-4 62-63 6.0747473360714246E-5 0.0 0.0 1.279250667766601 5.467272602464282E-4 64-65 6.0747473360714246E-5 0.0 0.0 1.4336707450495365 5.467272602464282E-4 66-67 6.0747473360714246E-5 0.0 0.0 1.6092309430620007 5.467272602464282E-4 68-69 6.0747473360714246E-5 0.0 0.0 1.806143877960756 5.467272602464282E-4 70-71 6.0747473360714246E-5 0.0 0.0 2.018213307463009 5.771009969267854E-4 72-73 6.0747473360714246E-5 0.0 0.0 2.2346261813105537 6.682222069678567E-4 74-75 6.0747473360714246E-5 0.0 0.0 2.453104469252363 6.985959436482138E-4 76-77 6.0747473360714246E-5 0.0 0.0 2.698524261629648 7.289696803285709E-4 78-79 6.0747473360714246E-5 0.0 0.0 2.9834602854280785 7.289696803285709E-4 80-81 6.0747473360714246E-5 0.0 0.0 3.2941836116681316 7.59343417008928E-4 82-83 6.0747473360714246E-5 0.0 0.0 3.6081873014696635 7.897171536892851E-4 84-85 6.0747473360714246E-5 0.0 0.0 3.9205508094904564 7.897171536892851E-4 86-87 6.0747473360714246E-5 0.0 0.0 4.253781074610654 7.897171536892851E-4 88-89 6.0747473360714246E-5 0.0 0.0 4.627985510512654 7.897171536892851E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1335 0.0 54.08129 2 ACCTGGG 1355 0.0 51.53031 3 CTGGGGG 1150 0.0 44.60778 5 GTACCTG 1630 0.0 44.32993 1 CCTGGGG 1460 0.0 40.666977 4 GTACACG 145 0.0 39.341805 1 GTACTAG 1625 0.0 31.301952 1 TATTCTC 830 0.0 30.330704 5 TAGGCAT 1885 0.0 29.986126 5 CAGTACG 240 0.0 27.707766 9 TGGGGGG 4980 0.0 26.801659 6 GTACCCG 160 6.196933E-8 26.740131 1 TAGTACT 2145 0.0 26.572882 4 CTGTGCG 2200 0.0 26.34037 9 TACCCCG 145 7.8924495E-7 26.206362 5 CTAGGCA 2175 0.0 26.20636 4 TTAAGGT 460 0.0 25.814688 4 GTACTGT 2340 0.0 24.96744 6 ACTGTGC 2460 0.0 24.908028 8 TACTGTG 2500 0.0 24.699495 7 >>END_MODULE