FastQCFastQC Report
Sat 18 Jun 2016
SRR1931085_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931085_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638869
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA53850.3285802587028005No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA53320.32534632115196516No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC44680.2726270373043849No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC32610.19897868591083243No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC31110.18982603246507193No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC30960.18891076712049593No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC25890.15797479847382553No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.15791378078418716No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT23810.1452831190290377No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC21360.13033378506762894No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC20970.12795409517173123No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19860.1211811316218685No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC19030.11611666338188104No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA18870.11514038034766659No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA17600.1073911337635894No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT17510.10684197455684376No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG20750.059.356962
ACCTGGG22700.053.8357163
GTACCTG23550.052.0985071
CCTGGGG21450.044.828964
TATCACG1650.043.0649262
CTGGGGG19900.037.3711555
GTACACG2750.036.174541
TCACGCA2600.030.9717314
TAGTACT11900.029.8541164
CTATTGA23750.029.1209589
CTGTGCG12050.028.6980429
TAACGCA1853.0013325E-1028.1650714
GCTATTG24950.027.7186588
TGGGGGG44350.027.235576
TCTAACG4050.026.9022882
GGGCTAT25900.026.8848446
TTTAGGG25100.026.6110382
GGCTATT26100.026.4973417
ATTTAGG25450.026.4312061
AGGGCTA25650.026.2235135
Produced by FastQC (version 0.11.5)