FastQCFastQC Report
Sat 18 Jun 2016
SRR1931085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1638869
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA57870.3531093699374386No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA57160.34877711397311195No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC48420.2954476532291477No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC40100.2446809354499963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35730.21801620507801417No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC35390.21594160363030845No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC30770.18775143101736624No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC30570.1865310772245982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27260.16633422195428677No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC25120.15327643637166852No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT24190.14760179123529704No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC23660.14436785368446167No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT20710.12636763524113276No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA19850.1211201139322301No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19820.12093706086331489No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC19500.11898449479488599No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA18040.11007591210767914No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC17340.10580467383299091No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG17130.10452330235058446No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA16750.10220463014432514No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC16650.1015944532479411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG21500.059.8689162
GTACCTG25000.052.478851
ACCTGGG23700.052.1069453
CCTGGGG18100.046.972654
TATCACG1250.045.597582
CTGGGGG14850.043.499385
TAACGCA1950.041.408064
GTACACG2700.036.9718131
TGGGGGG37750.030.574546
ATAACGC2800.028.8377553
TCACGCA2201.8189894E-1228.0666944
GTACCCG2053.092282E-1127.8254781
CTATTGA23400.025.7793339
GGGCTAT24450.025.0600226
ATCACGC2850.024.9986763
GCTATTG24550.024.9587048
AGGGCTA23850.024.694715
GGGGGGA41000.024.6755227
CATGGGG39200.024.596914
ATGGGGG32550.024.368915
Produced by FastQC (version 0.11.5)