##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931085_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1638869 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64710724286078 33.0 33.0 33.0 33.0 33.0 2 32.5987074012627 33.0 33.0 33.0 33.0 33.0 3 32.65040463880884 33.0 33.0 33.0 33.0 33.0 4 36.60088573278279 37.0 37.0 37.0 37.0 37.0 5 36.54938680272798 37.0 37.0 37.0 37.0 37.0 6 36.55047352778044 37.0 37.0 37.0 37.0 37.0 7 36.48959068723614 37.0 37.0 37.0 37.0 37.0 8 36.494530679389264 37.0 37.0 37.0 37.0 37.0 9 36.48933929435483 37.0 37.0 37.0 37.0 37.0 10-11 36.46511466139148 37.0 37.0 37.0 37.0 37.0 12-13 36.378280997443966 37.0 37.0 37.0 37.0 37.0 14-15 38.32894972081356 40.0 37.0 40.0 37.0 40.0 16-17 38.32441549629653 40.0 37.0 40.0 37.0 40.0 18-19 38.28673371697188 40.0 37.0 40.0 37.0 40.0 20-21 38.202633035343275 40.0 37.0 40.0 35.0 40.0 22-23 38.06766404148227 40.0 37.0 40.0 33.0 40.0 24-25 38.22020612995914 40.0 37.0 40.0 37.0 40.0 26-27 38.15775543987958 40.0 37.0 40.0 37.0 40.0 28-29 38.06590917272827 40.0 37.0 40.0 33.0 40.0 30-31 37.92531678859018 40.0 37.0 40.0 33.0 40.0 32-33 37.78266963375352 40.0 37.0 40.0 33.0 40.0 34-35 37.683223613357754 40.0 37.0 40.0 33.0 40.0 36-37 37.60435214773115 40.0 37.0 40.0 33.0 40.0 38-39 37.44471492230313 40.0 37.0 40.0 33.0 40.0 40-41 37.24963740237932 40.0 37.0 40.0 33.0 40.0 42-43 37.061266336723676 40.0 37.0 40.0 33.0 40.0 44-45 36.81315376640842 40.0 37.0 40.0 33.0 40.0 46-47 36.54428267298972 40.0 37.0 40.0 33.0 40.0 48-49 36.26417151096274 37.0 37.0 40.0 33.0 40.0 50-51 36.01743000813366 37.0 37.0 40.0 33.0 40.0 52-53 35.78697656737665 37.0 37.0 40.0 30.0 40.0 54-55 35.54921991934681 37.0 37.0 40.0 27.0 40.0 56-57 35.24107112893099 37.0 37.0 40.0 27.0 40.0 58-59 34.9663276930615 37.0 33.0 40.0 27.0 40.0 60-61 34.712693632010854 37.0 33.0 40.0 27.0 40.0 62-63 34.39456143230484 37.0 33.0 40.0 27.0 40.0 64-65 34.03808052992643 37.0 33.0 40.0 27.0 40.0 66-67 33.689614301082024 37.0 33.0 37.0 27.0 40.0 68-69 33.38815182909677 37.0 33.0 37.0 27.0 40.0 70-71 33.10759523793543 37.0 33.0 37.0 22.0 40.0 72-73 32.769455337796984 37.0 33.0 37.0 22.0 40.0 74-75 32.377997875364045 37.0 33.0 37.0 22.0 38.5 76-77 30.738744524425076 33.0 30.0 37.0 22.0 37.0 78-79 31.510957861793713 33.0 33.0 37.0 22.0 37.0 80-81 31.5586981021668 33.0 33.0 37.0 22.0 37.0 82-83 31.328597953832798 33.0 33.0 37.0 15.0 37.0 84-85 31.028713399301594 33.0 33.0 37.0 15.0 37.0 86-87 30.674294284656064 33.0 33.0 37.0 15.0 37.0 88-89 30.323523722762467 33.0 33.0 37.0 15.0 37.0 90-91 29.90882706305385 33.0 30.0 37.0 6.0 37.0 92-93 29.48819673811635 33.0 27.0 37.0 2.0 37.0 94-95 29.009671913984583 33.0 27.0 37.0 2.0 37.0 96-97 28.51366491159452 33.0 27.0 37.0 2.0 37.0 98-99 27.846877633294667 33.0 27.0 37.0 2.0 37.0 100-101 26.146534591843523 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.012444064607912253 1101 2 0.1624530257453145 1101 3 0.08085078736444018 1101 4 0.1451276938664563 1101 5 0.0717453782745423 1101 6 0.08893308564253033 1101 7 0.05046820915180916 1101 8 0.16548245029498787 1101 9 0.1153714496381042 1101 10-11 0.1346608991561027 1101 12-13 0.21240083481353622 1101 14-15 0.17338294278284394 1101 16-17 0.18041361839751602 1101 18-19 0.10277618207553729 1101 20-21 0.14719821993204363 1101 22-23 0.1617721914533945 1101 24-25 0.21556924570206348 1101 26-27 0.2128705606436725 1101 28-29 0.105693848189091 1101 30-31 0.026063553522710947 1101 32-33 0.18742763929959239 1101 34-35 0.11821732374999527 1101 36-37 0.15704251582061346 1101 38-39 0.010323706497160856 1101 40-41 -0.019510550640731594 1101 42-43 -0.08102752786064116 1101 44-45 -0.1132613696786322 1101 46-47 -0.1791444654226808 1101 48-49 -0.27187881185293605 1101 50-51 -0.0024360580577891255 1101 52-53 -0.36132285932588815 1101 54-55 -0.4737258392035102 1101 56-57 -0.499439199474665 1101 58-59 -0.4220487516125502 1101 60-61 -0.4933803413411013 1101 62-63 -0.696019960853107 1101 64-65 -0.6579412686984654 1101 66-67 -0.5985481905986703 1101 68-69 -0.8712315586919956 1101 70-71 -0.8767233872481413 1101 72-73 -0.9460621920437902 1101 74-75 -1.1057038937524126 1101 76-77 -1.1219337988895361 1101 78-79 -1.0918352956708013 1101 80-81 -1.0800409744287833 1101 82-83 -1.3108375870717985 1101 84-85 -1.1754347257042888 1101 86-87 -1.3370176071522408 1101 88-89 -1.6926652037534637 1101 90-91 -1.7624546646183283 1101 92-93 -1.831539588204457 1101 94-95 -1.8578783855273606 1101 96-97 -2.0819795100268124 1101 98-99 -2.4857438671942766 1101 100-101 -3.8968058768096974 1102 1 0.08540108657999212 1102 2 0.2104839350059038 1102 3 0.10801086572257645 1102 4 0.12354426548181152 1102 5 0.15206017166034513 1102 6 0.08236753425988752 1102 7 0.13163168315188756 1102 8 0.15035469535864365 1102 9 0.17542318881270802 1102 10-11 0.17874125992351253 1102 12-13 0.15809217160503408 1102 14-15 0.21577145920546315 1102 16-17 0.268428602253735 1102 18-19 0.32634283821415266 1102 20-21 0.1839358383209202 1102 22-23 0.11665672537556304 1102 24-25 0.2186554824103979 1102 26-27 0.2680393979742135 1102 28-29 0.2741661449275554 1102 30-31 0.2558054415198612 1102 32-33 0.2874659816415388 1102 34-35 0.22420355636663913 1102 36-37 0.12018674417178232 1102 38-39 0.17289086880985138 1102 40-41 0.30018435649402875 1102 42-43 0.2193205875029136 1102 44-45 0.09889476180894263 1102 46-47 0.1786295823287105 1102 48-49 0.12968228234738888 1102 50-51 -0.014165872354880094 1102 52-53 0.04141080253786811 1102 54-55 0.053653995023324796 1102 56-57 -9.216321077687439E-4 1102 58-59 0.03754812049827194 1102 60-61 0.05744303056903988 1102 62-63 -0.050776043332355414 1102 64-65 0.029623123678852892 1102 66-67 -0.08541961640509044 1102 68-69 -0.009083311750764267 1102 70-71 -0.06432685755190448 1102 72-73 -0.4057333122148492 1102 74-75 -0.36667525147129254 1102 76-77 -0.31249454874437177 1102 78-79 -0.07352820661312975 1102 80-81 -0.20447267793673873 1102 82-83 -0.3392175863454092 1102 84-85 -0.34626503049831925 1102 86-87 -0.3011318875598086 1102 88-89 -0.4635487924495578 1102 90-91 -0.5234696976667585 1102 92-93 -0.5461185511314568 1102 94-95 -0.6485186244819836 1102 96-97 -0.5638349391691051 1102 98-99 -0.848707003836914 1102 100-101 -1.669379700759503 1103 1 0.12426469716677957 1103 2 0.2680727515458088 1103 3 0.15408321269757153 1103 4 0.16470340752315593 1103 5 0.15787425301867586 1103 6 0.20132893457288503 1103 7 0.1495961681796132 1103 8 0.08305331834164775 1103 9 0.1501468991988375 1103 10-11 0.1889316428412826 1103 12-13 0.12435566480619542 1103 14-15 0.21446132872285517 1103 16-17 0.2019056273174229 1103 18-19 0.20027620095857657 1103 20-21 0.21562413421548143 1103 22-23 0.1484845298978854 1103 24-25 0.25755423968392677 1103 26-27 0.25645328190735484 1103 28-29 0.2453365899708544 1103 30-31 0.1713283315003764 1103 32-33 0.19489154936834296 1103 34-35 0.245535438982742 1103 36-37 0.21892090282694454 1103 38-39 0.2513714796980864 1103 40-41 0.22366678509126814 1103 42-43 0.25161564889749144 1103 44-45 0.4473551859927909 1103 46-47 0.25533560897204666 1103 48-49 0.2391715427705421 1103 50-51 0.10963455828906632 1103 52-53 0.21015522057557945 1103 54-55 0.1264973361061834 1103 56-57 0.20871498565109192 1103 58-59 0.13229108178128968 1103 60-61 0.05882237630591902 1103 62-63 0.06627967343796115 1103 64-65 0.14717132531129806 1103 66-67 0.1288820232417507 1103 68-69 0.05735945111518248 1103 70-71 0.16634149443073198 1103 72-73 -0.10716695203591797 1103 74-75 -0.12273417370568751 1103 76-77 -0.12897450672359767 1103 78-79 -0.11526759242154228 1103 80-81 -0.13307121350583273 1103 82-83 -0.014818842656183051 1103 84-85 -0.12182777639036502 1103 86-87 0.0749374449938287 1103 88-89 -0.02137026711827872 1103 90-91 -0.04634738762813129 1103 92-93 0.06637736818727191 1103 94-95 -0.01270686754870809 1103 96-97 -0.29570108161108166 1103 98-99 -0.1702347160291069 1103 100-101 -0.8181940067978744 1104 1 0.015474166822407653 1104 2 0.15044912219042317 1104 3 0.07133447697086126 1104 4 0.08302029802099753 1104 5 -0.023492430737213965 1104 6 0.017018287210994743 1104 7 0.09568538839977236 1104 8 0.0756092385477487 1104 9 0.0028355514877418386 1104 10-11 0.07627952630865309 1104 12-13 0.035133632359773515 1104 14-15 0.09367928150425087 1104 16-17 0.15718677986910734 1104 18-19 0.07317107568305659 1104 20-21 0.043166464264011495 1104 22-23 0.0596954332758699 1104 24-25 -0.024902959072278463 1104 26-27 0.10565911651298165 1104 28-29 0.2133110181633171 1104 30-31 0.16246321537490616 1104 32-33 0.02702682510012977 1104 34-35 0.01941406471959084 1104 36-37 0.057102580419034155 1104 38-39 0.09013792270138055 1104 40-41 0.1597058532200606 1104 42-43 0.19808222806413767 1104 44-45 0.17697085577270144 1104 46-47 0.24140453960445996 1104 48-49 0.34263011604764415 1104 50-51 0.28619988306946453 1104 52-53 0.13939800172309447 1104 54-55 0.20582587882002912 1104 56-57 0.22704125978122391 1104 58-59 0.171986262742422 1104 60-61 0.2902694653058475 1104 62-63 0.06616630466162121 1104 64-65 0.19964938956058376 1104 66-67 0.2603388583318633 1104 68-69 0.04309653140403924 1104 70-71 -0.014056218540467569 1104 72-73 -0.02906210746390059 1104 74-75 -0.21399684412634912 1104 76-77 -0.27680557878948875 1104 78-79 -0.1497117652463018 1104 80-81 -0.21660507331597856 1104 82-83 -0.1826082562274891 1104 84-85 -0.06084765801161396 1104 86-87 -0.2815372814209347 1104 88-89 -0.2282080756656022 1104 90-91 -0.2175094719543651 1104 92-93 -0.29516894970360497 1104 94-95 -0.08291476131793374 1104 96-97 -0.1515314384267228 1104 98-99 -0.47090482013909707 1104 100-101 -0.9798242538290403 1105 1 0.03721631925528612 1105 2 0.14932170877320772 1105 3 0.10702300910079998 1105 4 0.12533544760049864 1105 5 0.0784519361592686 1105 6 0.09426487098367176 1105 7 0.08791872008640667 1105 8 0.10250823509619522 1105 9 0.11143640616207762 1105 10-11 0.08398760282417328 1105 12-13 0.13830612929327657 1105 14-15 0.08948020265922452 1105 16-17 0.17736495254793994 1105 18-19 0.11181064306880018 1105 20-21 0.09961372571018501 1105 22-23 0.061924793740651296 1105 24-25 0.15693228155811312 1105 26-27 0.24531438994711863 1105 28-29 0.13136119801716006 1105 30-31 0.16890850461350482 1105 32-33 0.2301100174110573 1105 34-35 0.17570385592216553 1105 36-37 0.172885142810685 1105 38-39 0.3104785057165671 1105 40-41 0.17713346650040762 1105 42-43 0.21454307668016526 1105 44-45 0.3529086213266268 1105 46-47 0.13230488874263813 1105 48-49 0.2462598204807449 1105 50-51 0.33811757729457526 1105 52-53 0.08255017151522992 1105 54-55 0.22943588415456873 1105 56-57 0.2538592617855997 1105 58-59 0.23023244556338085 1105 60-61 0.07956646712909077 1105 62-63 0.05457585137172316 1105 64-65 0.2003371730175587 1105 66-67 0.5279796806543473 1105 68-69 0.23468845523316872 1105 70-71 0.11107920318836051 1105 72-73 0.02385145584797499 1105 74-75 0.18361686129936317 1105 76-77 0.09072564240526759 1105 78-79 0.45121126619374863 1105 80-81 0.2906790743093097 1105 82-83 0.43444207182913175 1105 84-85 0.33350826686374546 1105 86-87 0.14234622889409465 1105 88-89 0.36531458193580235 1105 90-91 0.264274606164971 1105 92-93 0.24740526753103964 1105 94-95 0.26253060719192334 1105 96-97 0.6050368744396906 1105 98-99 0.5459177275165246 1105 100-101 -0.35670278154353596 1106 1 0.12068288338483057 1106 2 0.16589292147701684 1106 3 0.1284815996201658 1106 4 0.1530305885553105 1106 5 0.10241998775162386 1106 6 0.1437491497497163 1106 7 0.13593577362831866 1106 8 0.1345612036132522 1106 9 0.11967310500651251 1106 10-11 0.15140575864012362 1106 12-13 0.16107004432239336 1106 14-15 0.22588998685906603 1106 16-17 0.1778371629648987 1106 18-19 0.1848700269016561 1106 20-21 0.2168618857760265 1106 22-23 0.17556391704828656 1106 24-25 0.15989877485608872 1106 26-27 0.2673817946112038 1106 28-29 0.3175453330963194 1106 30-31 0.3880191974811069 1106 32-33 0.2561591677695958 1106 34-35 0.23647902305092572 1106 36-37 0.3436819641599911 1106 38-39 0.45362572068699336 1106 40-41 0.3917227446255964 1106 42-43 0.3420550674518239 1106 44-45 0.4388542901452226 1106 46-47 0.38879778197586745 1106 48-49 0.33152247066052354 1106 50-51 0.43464750531621377 1106 52-53 0.449762915799802 1106 54-55 0.3838014694593781 1106 56-57 0.5217610959857808 1106 58-59 0.3414801604022557 1106 60-61 0.3908087852774784 1106 62-63 0.5542655416691531 1106 64-65 0.6374303131493733 1106 66-67 0.6062940918416828 1106 68-69 0.556224960471333 1106 70-71 0.6323252990200885 1106 72-73 0.6820857936403257 1106 74-75 0.7105463467923911 1106 76-77 0.7705855308286935 1106 78-79 0.8713065177807131 1106 80-81 0.8905684421544784 1106 82-83 0.7407885798331009 1106 84-85 0.7114756033946179 1106 86-87 0.9992276838418483 1106 88-89 0.9307966750422061 1106 90-91 1.0740378938288089 1106 92-93 1.2037713385379938 1106 94-95 1.1326052838964316 1106 96-97 1.0583807987780922 1106 98-99 1.1755833350826848 1106 100-101 0.972915267687771 1107 1 0.06667366863013058 1107 2 0.1463864136187354 1107 3 0.08548201847265346 1107 4 0.1411309823050928 1107 5 0.054498712107580616 1107 6 0.07730129617315384 1107 7 0.12327561944224641 1107 8 0.15390557502517765 1107 9 0.045706173730323485 1107 10-11 0.1413177427279919 1107 12-13 0.08030973816895681 1107 14-15 0.06664657382997774 1107 16-17 0.09686707500588199 1107 18-19 0.1354352999771251 1107 20-21 0.1096194711661056 1107 22-23 0.18872203635084617 1107 24-25 0.15105007162765816 1107 26-27 0.1898807510356093 1107 28-29 0.10145282856468185 1107 30-31 0.17169059952758658 1107 32-33 0.04039466953567228 1107 34-35 0.14324256624428955 1107 36-37 0.15993212505197363 1107 38-39 0.16039840183358223 1107 40-41 0.10163077485977823 1107 42-43 0.17536546701149547 1107 44-45 0.1945932447776002 1107 46-47 0.07130777505662422 1107 48-49 0.008157150935275581 1107 50-51 0.01366342242182128 1107 52-53 0.034922773091281556 1107 54-55 0.1523228917373558 1107 56-57 0.2647918955104629 1107 58-59 0.14877192707083964 1107 60-61 0.04534739686327072 1107 62-63 0.16017157506499302 1107 64-65 0.21259068956192806 1107 66-67 0.17222691330740503 1107 68-69 0.3534717342760558 1107 70-71 0.37717622238003656 1107 72-73 0.2879121810625236 1107 74-75 0.2367278272545903 1107 76-77 0.22540653005854594 1107 78-79 0.2983419168603767 1107 80-81 0.3989074467078133 1107 82-83 0.5264313975085315 1107 84-85 0.6309730799683884 1107 86-87 0.6853036120552254 1107 88-89 0.7039123188625673 1107 90-91 0.8511521922011163 1107 92-93 0.9160735480737472 1107 94-95 0.8444916632260266 1107 96-97 0.7115408950074951 1107 98-99 1.037182811125959 1107 100-101 1.777475959697501 1108 1 0.05678196526979207 1108 2 0.07486227406823787 1108 3 0.08272186707228002 1108 4 0.04866557872333033 1108 5 0.030045070843677024 1108 6 0.007768830398099169 1108 7 0.10644539561857869 1108 8 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0.5161574719472775 2316 84-85 0.3164028760695352 2316 86-87 0.43504880716297123 2316 88-89 0.12107308495304636 2316 90-91 0.4407212628706674 2316 92-93 0.5245508931029228 2316 94-95 0.57058979676302 2316 96-97 0.4625566805483139 2316 98-99 0.8864760194423482 2316 100-101 1.2328715391416871 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 14.0 5 31.0 6 64.0 7 126.0 8 409.0 9 790.0 10 1476.0 11 2162.0 12 3577.0 13 4862.0 14 5716.0 15 6621.0 16 8657.0 17 11032.0 18 11007.0 19 8739.0 20 7876.0 21 7751.0 22 8361.0 23 9380.0 24 10944.0 25 12740.0 26 15201.0 27 18776.0 28 22679.0 29 28355.0 30 35678.0 31 45702.0 32 58996.0 33 79092.0 34 113010.0 35 171088.0 36 274544.0 37 433698.0 38 219692.0 39 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.26993926847365 22.74589550757694 13.70926422946685 24.27490099448256 2 13.820532002006267 21.794094209044545 41.41021901729611 22.97515477165308 3 16.73147762267759 29.15956064822753 30.576452419320887 23.532509309773996 4 11.416653802103768 18.022489900046924 39.88988747727854 30.67096882057077 5 11.981555572776104 38.325393914949885 35.49411209803834 14.19893841423567 6 27.943050969906686 39.31070756723082 19.635614561017384 13.110626901845112 7 25.65543676767332 32.35810793907262 24.886308789781246 17.10014650347282 8 25.370484157061973 35.38421923899958 21.14879224636014 18.096504357578304 9 26.262440744196148 15.397325838733908 22.546829551355234 35.79340386571471 10-11 24.540826631048606 26.703598640281808 29.07846813869809 19.677106589971498 12-13 24.759636066091918 24.81586386709371 29.137167156130232 21.287332910684135 14-15 22.017527400864928 26.108047502459787 27.105721193815835 24.768703902859453 16-17 20.882327995709236 28.14276186809318 28.296465428292315 22.678444707905268 18-19 20.697139307656684 28.205732123800008 30.15482018391952 20.942308384623786 20-21 21.65822893715117 27.389010347989984 29.24159893194636 21.711161782912484 22-23 21.730321337458943 27.456068788902595 28.721179057020418 22.092430816618045 24-25 21.467365603962243 27.572795629180856 29.547633154327773 21.412205612529128 26-27 21.559685368385146 27.38055939797507 29.1568148521938 21.902940381445983 28-29 21.020411027360943 28.07695428981816 29.154160582694526 21.748474100126366 30-31 22.015212930380645 27.632440420802396 28.632154247837992 21.72019240097897 32-33 22.212147523688593 27.927125352911062 28.276085519953085 21.584641603447256 34-35 22.048162482785386 27.427939633979285 29.179330379670372 21.34456750356496 36-37 22.059237193454752 27.350904800810806 29.179910047721936 21.409947958012506 38-39 22.196004922913207 27.221122885505654 29.392409512181285 21.190462679399854 40-41 21.71483541226564 26.86283196100614 29.579569959397666 21.842762667330557 42-43 21.80399213801074 27.28770523764455 28.793406766412144 22.11489585793256 44-45 21.553003931369744 28.089890040021505 28.696466892716867 21.660639135891888 46-47 21.95620882450031 27.326589251489896 28.56192288706419 22.1552790369456 48-49 22.396082171402558 27.04032725047243 28.60119216507502 21.962398413049993 50-51 21.985771895130117 27.46415363277968 27.885358744353578 22.664715727736628 52-53 21.531617231151483 27.937223780546216 27.879684099217204 22.651474889085094 54-55 21.69572430743397 28.023197705246726 27.780255773951428 22.500822213367876 56-57 22.52751745258468 27.365671081703297 27.806371345116666 22.30044012059536 58-59 22.089562985205042 27.3732982929081 28.37551384521887 22.161624876667997 60-61 22.173523326147485 28.311170691495168 27.55317844196211 21.962127540395233 62-63 22.271060103034472 27.551408928962594 27.322226486680755 22.85530448132218 64-65 21.41559209430406 27.890941862955486 27.599124762259823 23.094341280480624 66-67 22.0228962334985 27.086757400179458 27.6286383840652 23.261707982256837 68-69 21.608682320024776 27.618418503324975 27.479652719403564 23.29324645724668 70-71 21.822483069646854 27.027943129512128 28.00649725279335 23.143076548047667 72-73 22.415763554011946 27.03071447443328 28.143341536144746 22.41018043541003 74-75 22.242280111113313 27.8341903784168 27.513694670252477 22.409834840217407 76-77 21.93702961983947 27.408602701676926 27.854895614809067 22.79947206367454 78-79 21.495702921915463 27.627948215388447 28.0276033263607 22.84874553633539 80-81 21.423131911684212 27.53363587159149 28.416354395683186 22.626877821041113 82-83 22.00878633118043 26.852343687787766 28.873431736906767 22.26543824412503 84-85 22.429758556335482 27.455052523767726 27.461032401479223 22.654156518417565 86-87 21.46418405420825 27.871697790849154 27.960509254880666 22.703608900061933 88-89 21.98143963916579 27.187743498717715 27.757130069578473 23.073686792538027 90-91 21.69792094426095 26.831918841591367 28.484247368154502 22.98591284599318 92-93 22.067726842269693 27.326268207911898 27.755350099582145 22.850654850236264 94-95 20.753554722157475 27.488305510231232 28.22881414883763 23.52932561877367 96-97 21.034153884079867 27.616275876881996 27.694838597086434 23.6547316419517 98-99 20.62504690734891 28.16173836957072 27.492862455754548 23.72035226732582 100-101 19.477827961529496 28.411779852654213 27.650430236517202 24.45996194929909 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 67.0 1 56.5 2 75.5 3 98.0 4 83.0 5 75.5 6 102.0 7 203.5 8 303.5 9 382.0 10 436.0 11 464.5 12 578.0 13 695.0 14 825.0 15 998.5 16 1179.0 17 1322.0 18 1414.0 19 1580.0 20 1797.5 21 2057.5 22 2348.5 23 2746.0 24 3383.5 25 4238.0 26 5977.5 27 8315.5 28 11776.0 29 18265.5 30 25734.5 31 31571.0 32 38738.0 33 44962.0 34 50324.5 35 55730.5 36 58561.5 37 63990.0 38 63172.0 39 54351.5 40 48244.0 41 44243.5 42 42643.5 43 43472.0 44 46503.5 45 52501.0 46 56738.5 47 59439.5 48 64537.5 49 64399.0 50 61074.0 51 59097.5 52 55336.0 53 52182.5 54 50222.0 55 46380.5 56 41195.0 57 34806.0 58 29501.0 59 27445.0 60 23187.0 61 17511.0 62 13194.5 63 10466.0 64 8134.0 65 5598.5 66 4096.5 67 3216.5 68 2652.0 69 2230.5 70 1818.5 71 1457.5 72 1151.5 73 955.0 74 778.0 75 669.5 76 574.5 77 446.5 78 349.0 79 291.5 80 236.0 81 175.5 82 149.0 83 137.5 84 112.0 85 97.0 86 84.5 87 60.5 88 47.5 89 27.0 90 15.0 91 14.5 92 10.5 93 8.0 94 5.0 95 2.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0813975979776297 2 1.830530689152092E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.9661498264962E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.2203537927680614E-4 40-41 0.002867831413004944 42-43 0.005979733584563501 44-45 0.0 46-47 0.0 48-49 1.2203537927680614E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0024712164303553243 68-69 0.00832891463564202 70-71 0.0013728980168640692 72-73 0.0 74-75 9.15265344576046E-5 76-77 0.0021356191373441075 78-79 0.004942432860710649 80-81 0.0041492028954114085 82-83 0.004484800188422625 84-85 0.0024101987407169215 86-87 2.4407075855361227E-4 88-89 0.0 90-91 0.0 92-93 0.0012813714824064645 94-95 0.0038441144472193935 96-97 0.0031119021715585566 98-99 0.0 100-101 1.830530689152092E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1638869.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.143279714408905 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75096712140953 39.10687581949873 2 9.016803897036286 8.321298087018352 3 2.2462275793233633 3.109449224848127 4 0.9570793848700375 1.766511270598102 5 0.5453657731635477 1.2582482708875231 6 0.35682981803677094 0.987917886246741 7 0.2469574677764343 0.7976799259219092 8 0.2003232688758782 0.7394858103235513 9 0.1548220331236849 0.6429596742341707 >10 1.1439449067341816 10.826603360018236 >50 0.187268480746585 6.117993941679484 >100 0.1715072989497086 15.699537443629804 >500 0.015663942148919786 4.926458083460119 >1k 0.005973537260181275 4.994387685272389 >5k 2.6549054489694555E-4 0.7045935163629798 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 5787 0.3531093699374386 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 5716 0.34877711397311195 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 4842 0.2954476532291477 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 4010 0.2446809354499963 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3573 0.21801620507801417 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 3539 0.21594160363030845 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 3077 0.18775143101736624 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 3057 0.1865310772245982 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2726 0.16633422195428677 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 2512 0.15327643637166852 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 2419 0.14760179123529704 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 2366 0.14436785368446167 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2071 0.12636763524113276 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 1985 0.1211201139322301 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1982 0.12093706086331489 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 1950 0.11898449479488599 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 1804 0.11007591210767914 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 1734 0.10580467383299091 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 1713 0.10452330235058446 No Hit ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA 1675 0.10220463014432514 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 1665 0.1015944532479411 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 3.0508844819201534E-5 0.0 0.0 0.0 0.0 18-19 6.101768963840307E-5 0.0 0.0 0.0 0.0 20-21 6.101768963840307E-5 0.0 0.0 9.15265344576046E-5 0.0 22-23 6.101768963840307E-5 0.0 0.0 2.1356191373441075E-4 0.0 24-25 6.101768963840307E-5 0.0 0.0 4.57632672288023E-4 0.0 26-27 6.101768963840307E-5 0.0 0.0 9.457741893952477E-4 3.0508844819201534E-5 28-29 6.101768963840307E-5 0.0 0.0 0.0025932518096321304 6.101768963840307E-5 30-31 6.101768963840307E-5 0.0 0.0 0.008115352721907608 6.101768963840307E-5 32-33 6.101768963840307E-5 0.0 0.0 0.022973160148858758 6.101768963840307E-5 34-35 6.101768963840307E-5 0.0 0.0 0.04655649719410154 6.101768963840307E-5 36-37 6.101768963840307E-5 0.0 0.0 0.08621799545906353 6.101768963840307E-5 38-39 6.101768963840307E-5 0.0 0.0 0.13735081937604532 6.101768963840307E-5 40-41 6.101768963840307E-5 0.0 0.0 0.19373116460192974 6.101768963840307E-5 42-43 6.101768963840307E-5 0.0 0.0 0.25169796975841263 6.101768963840307E-5 44-45 6.101768963840307E-5 0.0 0.0 0.3257429361346148 6.101768963840307E-5 46-47 6.101768963840307E-5 0.0 0.0 0.41513385145487525 6.101768963840307E-5 48-49 6.101768963840307E-5 0.0 0.0 0.5097722880840385 6.101768963840307E-5 50-51 6.101768963840307E-5 0.0 0.0 0.6069734676780145 1.2203537927680614E-4 52-53 6.101768963840307E-5 0.0 0.0 0.7026797138758498 1.2203537927680614E-4 54-55 6.101768963840307E-5 0.0 0.0 0.8097352503464279 1.2203537927680614E-4 56-57 6.101768963840307E-5 0.0 0.0 0.9391842789143 1.2203537927680614E-4 58-59 6.101768963840307E-5 0.0 0.0 1.088738636218026 1.2203537927680614E-4 60-61 6.101768963840307E-5 0.0 0.0 1.2426862671757168 1.2203537927680614E-4 62-63 6.101768963840307E-5 0.0 0.0 1.4002034329772544 1.2203537927680614E-4 64-65 6.101768963840307E-5 0.0 0.0 1.5634562616048018 1.2203537927680614E-4 66-67 6.101768963840307E-5 0.0 0.0 1.7476381577783213 1.2203537927680614E-4 68-69 6.101768963840307E-5 0.0 0.0 1.957996642806716 1.2203537927680614E-4 70-71 6.101768963840307E-5 0.0 0.0 2.187606208916027 1.2203537927680614E-4 72-73 6.101768963840307E-5 0.0 0.0 2.431371879021447 1.2203537927680614E-4 74-75 6.101768963840307E-5 0.0 0.0 2.6684866209562816 1.2203537927680614E-4 76-77 6.101768963840307E-5 0.0 0.0 2.9343406947108037 1.2203537927680614E-4 78-79 6.101768963840307E-5 0.0 0.0 3.2270730607510423 1.2203537927680614E-4 80-81 6.101768963840307E-5 0.0 0.0 3.541893830440383 1.2203537927680614E-4 82-83 6.101768963840307E-5 0.0 0.0 3.8762707696588317 1.2203537927680614E-4 84-85 6.101768963840307E-5 0.0 0.0 4.213240960686912 1.2203537927680614E-4 86-87 6.101768963840307E-5 0.0 0.0 4.568607985140972 1.2203537927680614E-4 88-89 6.101768963840307E-5 0.0 0.0 4.947680382019551 1.2203537927680614E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2150 0.0 59.868916 2 GTACCTG 2500 0.0 52.47885 1 ACCTGGG 2370 0.0 52.106945 3 CCTGGGG 1810 0.0 46.97265 4 TATCACG 125 0.0 45.59758 2 CTGGGGG 1485 0.0 43.49938 5 TAACGCA 195 0.0 41.40806 4 GTACACG 270 0.0 36.971813 1 TGGGGGG 3775 0.0 30.57454 6 ATAACGC 280 0.0 28.837755 3 TCACGCA 220 1.8189894E-12 28.066694 4 GTACCCG 205 3.092282E-11 27.825478 1 CTATTGA 2340 0.0 25.779333 9 GGGCTAT 2445 0.0 25.060022 6 ATCACGC 285 0.0 24.998676 3 GCTATTG 2455 0.0 24.958704 8 AGGGCTA 2385 0.0 24.69471 5 GGGGGGA 4100 0.0 24.675522 7 CATGGGG 3920 0.0 24.59691 4 ATGGGGG 3255 0.0 24.36891 5 >>END_MODULE