FastQCFastQC Report
Sat 18 Jun 2016
SRR1931084_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931084_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537932
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63770.4146477217458249No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA40120.26086979138219374No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34000.2210760943916896No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA33620.21860524392495898No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC32530.21151780442828422No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC29160.18960526213122555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28540.1855738745276124No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC27010.17562545027998636No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC24990.16249092937789186No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC24720.16073532509889904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20860.13563668614737193No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT20560.1336860147262688No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT20200.13134520902094501No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT17990.1169752628854852No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA17160.11157840528709982No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC17160.11157840528709982No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA16660.10832728625192792No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC16050.10436092102901817No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC16040.10429589864831475No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC15730.10228020484650817No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15680.10195509294299097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG22550.054.8128972
ACCTGGG22900.054.5920033
GTACCTG24900.050.400561
CCTGGGG21850.045.945714
CTGGGGG20150.041.1266675
GTACACG3050.037.264921
ATAACGC1850.033.276083
TAACGCA1900.032.4003944
TGGGGGG45550.030.2517876
TATCACG1102.79903E-630.1365912
GTACTAG10500.028.41451
CATGGGG40450.026.4576024
CTATTGA17800.025.80559
ATGGGGG35250.025.7930475
TAGTACT12250.025.1268354
TACACGG4750.024.9250012
AGGGCTA18250.024.9097235
GTACATG66900.024.7051851
GGGGGGA45950.024.526637
TAGGCAT12750.024.5128775
Produced by FastQC (version 0.11.5)