##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931084_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1537932 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.33916193953959 33.0 33.0 33.0 27.0 33.0 2 30.221403156966627 33.0 33.0 33.0 27.0 33.0 3 30.261454342584717 33.0 33.0 33.0 27.0 33.0 4 33.747988857764845 37.0 37.0 37.0 27.0 37.0 5 33.69347994579734 37.0 37.0 37.0 27.0 37.0 6 33.80422086282098 37.0 37.0 37.0 27.0 37.0 7 33.76112467911455 37.0 37.0 37.0 27.0 37.0 8 33.754113966027106 37.0 37.0 37.0 27.0 37.0 9 33.785655022458734 37.0 37.0 37.0 27.0 37.0 10-11 33.64151958604151 37.0 37.0 37.0 27.0 37.0 12-13 33.52091282319374 37.0 37.0 37.0 27.0 37.0 14-15 34.91615689120195 37.0 37.0 40.0 27.0 40.0 16-17 34.843260950419136 37.0 37.0 40.0 27.0 40.0 18-19 34.756208336909566 37.0 37.0 40.0 24.5 40.0 20-21 34.65101512940754 37.0 37.0 40.0 22.0 40.0 22-23 34.51343589963666 37.0 37.0 40.0 22.0 40.0 24-25 34.34749975941719 37.0 35.0 40.0 22.0 40.0 26-27 34.12221899277732 37.0 33.0 40.0 15.0 40.0 28-29 33.969319189665086 37.0 33.0 40.0 15.0 40.0 30-31 33.77800676492849 37.0 33.0 40.0 15.0 40.0 32-33 33.569363274839205 37.0 33.0 40.0 15.0 40.0 34-35 33.38057534403342 37.0 33.0 40.0 15.0 40.0 36-37 33.100555486198346 37.0 33.0 40.0 6.0 40.0 38-39 32.85205620274499 37.0 33.0 40.0 6.0 40.0 40-41 32.58728084206584 37.0 33.0 40.0 6.0 40.0 42-43 32.33782442916852 37.0 33.0 40.0 6.0 40.0 44-45 31.982760941316002 37.0 33.0 40.0 2.0 40.0 46-47 31.768235201556376 37.0 33.0 40.0 2.0 40.0 48-49 31.67256972349883 37.0 33.0 40.0 2.0 40.0 50-51 30.84604488364895 37.0 30.0 38.5 2.0 40.0 52-53 30.984543529882984 37.0 33.0 37.0 2.0 40.0 54-55 31.403142986816064 37.0 33.0 40.0 2.0 40.0 56-57 31.293128694896783 37.0 33.0 40.0 2.0 40.0 58-59 31.06665671824242 37.0 33.0 40.0 2.0 40.0 60-61 31.032383746485543 37.0 33.0 40.0 2.0 40.0 62-63 30.82421719555871 37.0 33.0 38.5 2.0 40.0 64-65 30.564255116611136 37.0 33.0 37.0 2.0 40.0 66-67 30.264631986329697 37.0 27.0 37.0 2.0 40.0 68-69 29.972445790841206 37.0 27.0 37.0 2.0 40.0 70-71 29.69822820514821 37.0 27.0 37.0 2.0 40.0 72-73 29.10111825490334 35.0 27.0 37.0 2.0 40.0 74-75 28.922689689791227 33.0 27.0 37.0 2.0 38.5 76-77 28.593488853863498 33.0 27.0 37.0 2.0 37.0 78-79 28.32746311280343 33.0 27.0 37.0 2.0 37.0 80-81 28.08147596902854 33.0 27.0 37.0 2.0 37.0 82-83 27.802507523089446 33.0 27.0 37.0 2.0 37.0 84-85 27.40326295310846 33.0 24.5 37.0 2.0 37.0 86-87 27.239868537750695 33.0 27.0 37.0 2.0 37.0 88-89 27.0377968596791 33.0 22.0 37.0 2.0 37.0 90-91 26.723416900097014 33.0 22.0 37.0 2.0 37.0 92-93 26.39978393062892 33.0 22.0 37.0 2.0 37.0 94-95 26.09958112582351 33.0 18.5 37.0 2.0 37.0 96-97 25.74548614633157 33.0 15.0 37.0 2.0 37.0 98-99 25.373318521235007 33.0 8.5 37.0 2.0 37.0 100-101 24.642979988712113 33.0 2.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34579431407888706 1101 2 0.43967550648302733 1101 3 0.3369468787980594 1101 4 0.3592057078505704 1101 5 0.3358187211822923 1101 6 0.43834484655373984 1101 7 0.3593335715885857 1101 8 0.34515209521816104 1101 9 0.24932538971212637 1101 10-11 0.4575410899654173 1101 12-13 0.4447480185130601 1101 14-15 0.7409580099128803 1101 16-17 0.620641591451438 1101 18-19 0.633822429696103 1101 20-21 0.633183830154735 1101 22-23 0.6316899877448208 1101 24-25 0.7251578025859402 1101 26-27 0.6359630053772491 1101 28-29 0.5745157762135733 1101 30-31 0.5420316973879338 1101 32-33 0.284507199014584 1101 34-35 0.456417561851417 1101 36-37 0.24163751742760553 1101 38-39 0.487391799991741 1101 40-41 0.5532607377095857 1101 42-43 0.4865413867713144 1101 44-45 0.25261512023936916 1101 46-47 0.15625112971996558 1101 48-49 0.18693057258464663 1101 50-51 0.2962245636164411 1101 52-53 0.43543733006035623 1101 54-55 0.7104134737199352 1101 56-57 0.5560991234614541 1101 58-59 0.4828579222655378 1101 60-61 0.5279349960177235 1101 62-63 0.5194074181881128 1101 64-65 0.6834669891271261 1101 66-67 0.8249452300067261 1101 68-69 0.6132018273140858 1101 70-71 0.6518720685059094 1101 72-73 0.5144546673642836 1101 74-75 0.8121543975262391 1101 76-77 0.580937380734543 1101 78-79 0.319087636159086 1101 80-81 0.42959510305807314 1101 82-83 0.4045728333544112 1101 84-85 0.5998748342671867 1101 86-87 0.48643371390975787 1101 88-89 0.3720289051640613 1101 90-91 0.29133834540985504 1101 92-93 0.13637543877417357 1101 94-95 0.3141415893166517 1101 96-97 0.23083591950886273 1101 98-99 0.18650090035839284 1101 100-101 0.21082391197396433 1102 1 0.5097170116831506 1102 2 0.5370919006646702 1102 3 0.5891104493069861 1102 4 0.6346871875058824 1102 5 0.43622519722767805 1102 6 0.41989619742371787 1102 7 0.5058345016182528 1102 8 0.37431683952163297 1102 9 0.4168399086903207 1102 10-11 0.5008877175827422 1102 12-13 0.5719759263462905 1102 14-15 0.6558553896794379 1102 16-17 0.6468585165323475 1102 18-19 0.5909972928833795 1102 20-21 0.6843638886281198 1102 22-23 0.6801501433820292 1102 24-25 0.8861442528813015 1102 26-27 0.8092493936370815 1102 28-29 0.8739845750640285 1102 30-31 0.8749481064118143 1102 32-33 0.936758615441974 1102 34-35 0.7966202514660239 1102 36-37 0.8127066767180224 1102 38-39 0.8053331464895521 1102 40-41 0.8030601159456339 1102 42-43 0.7990024258989692 1102 44-45 0.8774712888995069 1102 46-47 0.8369988866554401 1102 48-49 0.908699505618987 1102 50-51 0.7306596102135146 1102 52-53 0.946470143479349 1102 54-55 0.9389600262707596 1102 56-57 0.984404577706858 1102 58-59 0.9831416857134876 1102 60-61 0.9939108026681573 1102 62-63 0.947886451138384 1102 64-65 1.0147671036890742 1102 66-67 1.0726156276931462 1102 68-69 1.1647569667279747 1102 70-71 1.1854547681931145 1102 72-73 0.9497176089145469 1102 74-75 1.0093835782699685 1102 76-77 1.0486522447570117 1102 78-79 0.969942876047373 1102 80-81 0.947314218500992 1102 82-83 0.8694420128705111 1102 84-85 0.9922477356629109 1102 86-87 1.0681789698024993 1102 88-89 1.026799225318662 1102 90-91 1.0574775172935276 1102 92-93 1.0642756244782312 1102 94-95 1.0908562324665034 1102 96-97 1.3287546856812398 1102 98-99 1.1087489609308214 1102 100-101 0.9086804916885995 1103 1 1.6293731637517794E-4 1103 2 -0.04145414928391844 1103 3 0.06360749769705976 1103 4 -0.08434132243206705 1103 5 0.05486564988764542 1103 6 -0.07950099743228378 1103 7 -0.15872754836037473 1103 8 -0.06393962931200292 1103 9 -0.14839937609040987 1103 10-11 -0.30498673469521975 1103 12-13 -0.1436154914354475 1103 14-15 -0.06505943810694248 1103 16-17 -0.034998808477006094 1103 18-19 -0.03201836597161645 1103 20-21 0.044503709386859214 1103 22-23 -0.12997867248716233 1103 24-25 -0.13935846408278962 1103 26-27 -0.16682142435236358 1103 28-29 -0.114778705303479 1103 30-31 -0.04727970559557804 1103 32-33 -0.21134631442004093 1103 34-35 -0.253868023047076 1103 36-37 -0.044827636715311314 1103 38-39 -0.025034811026415582 1103 40-41 -0.09983053299557554 1103 42-43 -0.3719471529352205 1103 44-45 -0.28472459250298243 1103 46-47 -0.2632519028104632 1103 48-49 -0.386481197483171 1103 50-51 -0.30025591681744856 1103 52-53 -0.05054258042557436 1103 54-55 0.016482316451632784 1103 56-57 0.0754431717841193 1103 58-59 0.1415764290649122 1103 60-61 0.23174969577805982 1103 62-63 0.1245219677886169 1103 64-65 0.21817805629952858 1103 66-67 0.2875218796436414 1103 68-69 0.07875621867706073 1103 70-71 0.07193874587172644 1103 72-73 -0.1302168404256534 1103 74-75 -0.05764558492198191 1103 76-77 0.0807259836177856 1103 78-79 -0.05925574742631312 1103 80-81 0.08830609459297278 1103 82-83 0.2518105372908117 1103 84-85 0.3634349168672131 1103 86-87 0.2603452248221636 1103 88-89 0.23319651544086994 1103 90-91 0.12212901426354605 1103 92-93 -0.07285679695882408 1103 94-95 -0.04384197310295335 1103 96-97 0.001920963696193212 1103 98-99 -0.034981701319498626 1103 100-101 0.05505138884755567 1104 1 0.09415779324178786 1104 2 0.20781227043037376 1104 3 0.17882310776926147 1104 4 0.251457529030219 1104 5 0.27135561787403617 1104 6 0.10245762298726646 1104 7 0.018471534625327024 1104 8 0.25313617925080223 1104 9 0.25200127245998516 1104 10-11 0.10763387266875668 1104 12-13 0.11980314179309914 1104 14-15 0.333294573741604 1104 16-17 0.3845977657598141 1104 18-19 0.2895401488782383 1104 20-21 0.2460127506832137 1104 22-23 0.3007398823652423 1104 24-25 0.314963061497167 1104 26-27 0.2981879198114967 1104 28-29 0.08989876193186319 1104 30-31 0.11970243879544995 1104 32-33 0.1651197891192595 1104 34-35 0.033954699983361536 1104 36-37 0.20328717432962407 1104 38-39 0.05650286582618236 1104 40-41 0.014966937426443394 1104 42-43 0.09651501443902788 1104 44-45 -0.06347352935121009 1104 46-47 0.10974335233928656 1104 48-49 0.21874890839361427 1104 50-51 0.21075902538848723 1104 52-53 0.3355242676203858 1104 54-55 0.4939702028636255 1104 56-57 0.5004989274463654 1104 58-59 0.43197630765086714 1104 60-61 0.4447648097795316 1104 62-63 0.3312264888151013 1104 64-65 0.28931943289803996 1104 66-67 0.42218932843989165 1104 68-69 0.423821569965547 1104 70-71 0.2553218005120499 1104 72-73 0.29513176876487535 1104 74-75 0.16136234482165435 1104 76-77 0.3571320922397412 1104 78-79 0.2800804898153082 1104 80-81 0.31190087610332284 1104 82-83 -0.0885279889260353 1104 84-85 0.06283523473346264 1104 86-87 0.1696765707867698 1104 88-89 0.05138676964930511 1104 90-91 0.2895370869624294 1104 92-93 0.14193524365613186 1104 94-95 0.015016022755258263 1104 96-97 0.09980235979750063 1104 98-99 0.17798512300443647 1104 100-101 0.22491667177387953 1105 1 0.050440002656813476 1105 2 0.045578229003314874 1105 3 0.044917473124961305 1105 4 0.09826149550681151 1105 5 -0.03841538115081988 1105 6 -0.10073686503467627 1105 7 -0.04567482139977841 1105 8 -0.06810582807190713 1105 9 0.015612376866869226 1105 10-11 -0.05296871200249598 1105 12-13 0.024915702993901334 1105 14-15 0.05115354186320076 1105 16-17 0.049528601393397764 1105 18-19 0.02339039952470756 1105 20-21 0.22717838059362094 1105 22-23 5.9607663992267135E-5 1105 24-25 0.07397238235300563 1105 26-27 0.011303256986991528 1105 28-29 0.06224042530639906 1105 30-31 0.16117522971281772 1105 32-33 0.012525019180912977 1105 34-35 -0.04245806831463739 1105 36-37 0.10377847442663324 1105 38-39 0.32177316133029876 1105 40-41 0.1296662230660246 1105 42-43 0.10913413978681774 1105 44-45 0.2123320050683759 1105 46-47 0.3676204345849463 1105 48-49 0.262383284241789 1105 50-51 0.3059744068965813 1105 52-53 0.34299475595227236 1105 54-55 0.4512444971940077 1105 56-57 0.4235371885582033 1105 58-59 0.4258684011617504 1105 60-61 0.49235439911226564 1105 62-63 0.5800327477149629 1105 64-65 0.5167382803096245 1105 66-67 0.6625131131146986 1105 68-69 0.6423072071758043 1105 70-71 0.7763793638786716 1105 72-73 0.5502942729514899 1105 74-75 0.5253143910867699 1105 76-77 0.5391758633818817 1105 78-79 0.5469506969082687 1105 80-81 0.6105584492934035 1105 82-83 0.5089251940236537 1105 84-85 0.5437061608021772 1105 86-87 0.6787173309533117 1105 88-89 0.5551120633263373 1105 90-91 0.5132698075150195 1105 92-93 0.46600700065418366 1105 94-95 0.5681493046847912 1105 96-97 0.4479197908099195 1105 98-99 0.5059720798188891 1105 100-101 0.5379541834233628 1106 1 -0.049754688627373866 1106 2 -0.07437045391098351 1106 3 -0.23199042221554222 1106 4 -0.2026750079411599 1106 5 -0.1426444528804609 1106 6 -0.29356131505682015 1106 7 -0.004795907867233495 1106 8 -0.1748215095623067 1106 9 -0.07103483735443916 1106 10-11 -0.08867921423760095 1106 12-13 -0.09246378902367525 1106 14-15 -0.028080764175811623 1106 16-17 -0.04616270698374336 1106 18-19 -0.22301898936654396 1106 20-21 -0.05608930490738118 1106 22-23 0.0209703608122922 1106 24-25 0.06253885724792241 1106 26-27 0.12832385472427177 1106 28-29 0.15343564961428058 1106 30-31 0.3124978625328936 1106 32-33 0.15441838774886207 1106 34-35 0.12813453892197657 1106 36-37 0.0936412229103496 1106 38-39 -0.02288734261482972 1106 40-41 0.2668326557528147 1106 42-43 0.22728355492051833 1106 44-45 0.20867737272543252 1106 46-47 0.01820192256355213 1106 48-49 0.022474865887421203 1106 50-51 0.22770062790872458 1106 52-53 0.2709564783445231 1106 54-55 0.30882144154183067 1106 56-57 0.2608702849921123 1106 58-59 0.08411033219232067 1106 60-61 -0.0563534563557333 1106 62-63 0.110615920682406 1106 64-65 0.03814076658571963 1106 66-67 0.05874032301509402 1106 68-69 0.18167359298319496 1106 70-71 0.24598846315203815 1106 72-73 0.06463002449404698 1106 74-75 0.2550930653008514 1106 76-77 0.19188777783783806 1106 78-79 0.12573560307858145 1106 80-81 0.27663536986786497 1106 82-83 0.2730561610320734 1106 84-85 0.36074077230798096 1106 86-87 0.26426971950840183 1106 88-89 0.28852347337004147 1106 90-91 0.409146047587555 1106 92-93 0.41505297999555424 1106 94-95 0.3270407488620286 1106 96-97 0.2571834340060235 1106 98-99 0.2619072861591185 1106 100-101 0.3640562453658909 1107 1 -0.22109628330177244 1107 2 -0.21923960872786452 1107 3 -0.307609718173822 1107 4 -0.16076230846470452 1107 5 -0.2553179349504262 1107 6 -0.362240922054184 1107 7 -0.2777243786255781 1107 8 -0.44966178624006403 1107 9 -0.3338449930364433 1107 10-11 -0.3734595007444881 1107 12-13 -0.3875662840786518 1107 14-15 -0.24209153559986163 1107 16-17 -0.283123678848888 1107 18-19 -0.3648002953407854 1107 20-21 -0.310723210523399 1107 22-23 -0.2621111518985515 1107 24-25 -0.20857914508344777 1107 26-27 -0.2670489413375776 1107 28-29 -0.43057353777682295 1107 30-31 -0.3179291765033625 1107 32-33 -0.2795666329857198 1107 34-35 -0.3402331354333441 1107 36-37 -0.21758530598606285 1107 38-39 -0.2871582609883845 1107 40-41 -0.1946083268350165 1107 42-43 -0.15681559741877038 1107 44-45 -0.4793865328499578 1107 46-47 -0.6959022661881882 1107 48-49 -0.3399516695075917 1107 50-51 -0.4809946975694288 1107 52-53 -0.30243907905230216 1107 54-55 -0.13810918999991983 1107 56-57 -0.2007875882608765 1107 58-59 -0.3338203115342999 1107 60-61 -0.2745024123155524 1107 62-63 -0.3411062074455522 1107 64-65 -0.31195265074197565 1107 66-67 -0.16974871263376912 1107 68-69 -0.2728673014674108 1107 70-71 -0.2685121399989683 1107 72-73 -0.3249259182695603 1107 74-75 -0.2816934598588716 1107 76-77 0.01916462211927339 1107 78-79 -0.10362302604244178 1107 80-81 0.0419795721042 1107 82-83 0.2421096404213685 1107 84-85 0.3136082781059457 1107 86-87 0.2550742051647781 1107 88-89 0.17994329490232985 1107 90-91 0.07260992148228596 1107 92-93 0.05982729591670832 1107 94-95 0.06039642112154908 1107 96-97 0.27235339261808633 1107 98-99 0.1420577286729383 1107 100-101 0.21623104137087878 1108 1 0.11386903467170484 1108 2 0.10385758565148251 1108 3 0.14314556883524077 1108 4 0.17653145818219684 1108 5 0.15445138486774113 1108 6 0.06223696521275457 1108 7 0.06739613788720789 1108 8 0.10265301550298034 1108 9 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-0.28919631147179814 2316 98-99 -0.28857777733736256 2316 100-101 -0.38965232439644737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 70750.0 3 10328.0 4 7798.0 5 6746.0 6 6570.0 7 7088.0 8 7541.0 9 7593.0 10 7628.0 11 7755.0 12 8550.0 13 9193.0 14 9275.0 15 10090.0 16 11979.0 17 14815.0 18 12724.0 19 10290.0 20 10083.0 21 10370.0 22 11090.0 23 12643.0 24 14299.0 25 15983.0 26 18167.0 27 21414.0 28 25175.0 29 30421.0 30 36777.0 31 45122.0 32 56794.0 33 73052.0 34 99400.0 35 140579.0 36 205479.0 37 297107.0 38 187095.0 39 169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.345970956895705 20.139005839701777 11.901261508230917 23.613761695171597 2 15.847033976554798 20.901381378569514 40.122987169383386 23.1285974754923 3 19.429727712278567 28.082646046769298 28.726042503829817 23.76158373712232 4 12.545995655162145 16.598815150622563 39.66325881045596 31.19193038375933 5 12.954950666942363 37.98606424026311 34.37552424908025 14.683460843714277 6 28.55288790401656 37.90291118202885 19.25858880626712 14.285612107687465 7 26.552604406436696 31.70777381574738 24.18793548739476 17.551686290421163 8 26.044779613142843 33.35934228561471 20.938832146024662 19.657045955217786 9 26.930969639750003 15.248723610666792 21.98764314677112 35.83266360281209 10-11 25.617420015969493 25.55298283669239 28.200791712507446 20.628805434830667 12-13 26.222528405885964 23.93500482304293 27.902514387037897 21.93995238403321 14-15 23.07373687357373 25.15657100795543 26.503002021288392 25.266690097182455 16-17 21.775490908807164 27.183648467807902 27.549903697470906 23.490956925914027 18-19 21.49828693080033 27.834757260467796 28.998402349203417 21.668553459528457 20-21 22.85136794084524 26.587293846541982 28.01134900632798 22.549989206284803 22-23 22.808908326245895 26.65039806701467 27.69140638207671 22.849287224662728 24-25 22.843012564924848 26.639376773485434 28.476161494786506 22.041449166803215 26-27 22.70061355118432 26.373467747598724 28.343873461245362 22.5820452399716 28-29 21.852721706811483 27.514220394659844 28.26688696249249 22.366170936036184 30-31 22.90569413992296 26.683949615457642 27.855295292639724 22.555060951979673 32-33 23.145399146386186 27.00993932111433 27.400073605334956 22.44458792716453 34-35 23.152486585882862 26.459980025124647 28.291172821685223 22.096360567307265 36-37 23.086423847088167 26.296058603371282 28.21994080362461 22.397576745915945 38-39 23.06870524834648 26.611417149782955 28.3210180944281 21.99885950744246 40-41 22.720774911983938 26.122978979205485 28.425165132732026 22.73108097607855 42-43 22.719242463255853 26.52727818915271 27.427480538801458 23.325998808789986 44-45 22.724082179640277 26.939766509842656 27.456079577430216 22.880071733086854 46-47 23.159439276824315 26.67391589152365 27.276857067994236 22.8897877636578 48-49 23.633736940246674 26.273943902506787 27.326209063914142 22.766110093332394 50-51 22.716762798216454 26.743743832297916 27.2808705016784 23.25862286780723 52-53 22.577289218118597 27.33741836541953 26.829578607507106 23.255713808954773 54-55 22.494740904104557 26.94190219924642 26.957070315800184 23.606286580848835 56-57 23.498571232451006 26.240180993527417 26.81602502291604 23.44522275110553 58-59 23.30359067369847 26.40000991313291 27.343714079159625 22.95268533400899 60-61 22.948409821186388 27.29545063325474 27.05866474012561 22.697474805433263 62-63 22.852610639980544 26.776867101626635 26.752352519688273 23.618169738704548 64-65 22.09024370605583 27.365059682084553 26.910969266260953 23.633727345598665 66-67 22.91805599829467 26.28618699605153 26.62330974158595 24.17244726406786 68-69 22.37802436009694 26.71043537229198 26.729792059000744 24.181748208610337 70-71 22.825743677223993 26.31752542913136 26.984652078248295 23.872078815396353 72-73 23.40122091445339 26.074265740317053 27.499485385606793 23.025027959622765 74-75 23.10568877670266 27.23575681085147 26.672504796190115 22.98604961625576 76-77 23.114028601380205 26.46416189737059 26.707130975580068 23.71467852566913 78-79 22.754835084733486 26.64809223221458 27.058235061110302 23.53883762194163 80-81 22.69321958171495 26.645403462771455 27.447241490641904 23.21413546487169 82-83 23.178869272075925 25.75803075847583 28.2324946110553 22.830605358392944 84-85 23.331240210903275 26.560517320751124 26.921499432053075 23.186743036292526 86-87 22.48957892297448 27.085087291289188 27.205880195993497 23.219453589742837 88-89 23.085548182232728 26.277499073490684 26.78271963159969 23.854233112676894 90-91 23.11537902352488 25.824296545298903 27.259175276417164 23.801149154759056 92-93 23.562565209089424 26.340562024963393 27.105801482840846 22.99107128310634 94-95 22.332330082540178 26.335365893381802 27.602328323593984 23.729975700484037 96-97 22.357792797441935 26.499708111758196 27.38914514350385 23.75335394729602 98-99 22.37225948189714 27.131190271594214 26.631921119837294 23.864629126671353 100-101 22.582439104122177 26.343688126105608 26.57222572964187 24.501647040130344 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 139.0 1 100.5 2 130.0 3 161.0 4 108.5 5 104.5 6 154.5 7 249.0 8 381.5 9 462.5 10 431.5 11 401.5 12 460.0 13 571.0 14 701.0 15 861.0 16 1018.5 17 1171.5 18 1404.0 19 1617.0 20 1811.5 21 2066.0 22 2361.0 23 2741.0 24 3149.5 25 3660.5 26 4578.5 27 5809.5 28 7534.5 29 10843.5 30 15492.5 31 19700.5 32 24814.0 33 30128.0 34 35073.5 35 39570.5 36 42840.0 37 49163.0 38 51241.0 39 46816.5 40 43378.5 41 40463.5 42 39349.5 43 41046.0 44 44087.5 45 49444.0 46 54926.5 47 61723.0 48 72141.5 49 71922.0 50 63620.5 51 59891.0 52 57040.0 53 54781.5 54 52759.5 55 48186.0 56 43560.5 57 37387.5 58 31233.0 59 28651.0 60 24965.5 61 20252.5 62 16438.5 63 13674.0 64 11107.5 65 8274.5 66 6382.5 67 5200.5 68 4334.0 69 3660.0 70 3062.0 71 2540.0 72 2136.5 73 1814.0 74 1527.0 75 1251.0 76 1024.5 77 872.5 78 743.5 79 611.5 80 490.0 81 398.0 82 339.5 83 274.5 84 206.5 85 175.5 86 145.0 87 113.0 88 86.0 89 68.0 90 60.0 91 41.0 92 29.0 93 28.5 94 22.0 95 12.5 96 9.5 97 8.5 98 4.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.152461877378193E-4 2 0.0031210742737650303 3 0.0 4 9.753357105515719E-4 5 0.0011704028526618863 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.625559517585953E-4 14-15 0.0033486526062270635 16-17 0.004551566649240669 18-19 0.003186096654468468 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.625559517585953E-4 42-43 0.0 44-45 5.52690235979224E-4 46-47 0.7368336181313608 48-49 0.5321756748672893 50-51 0.40726768153598475 52-53 0.34224530083254656 54-55 0.33236189896562396 56-57 0.3002408428981255 58-59 0.29991573099460833 60-61 0.27751552084227393 62-63 0.26990790229997164 64-65 0.2663641825516343 66-67 0.2549527547381809 68-69 0.23268258934725333 70-71 0.2480928935739682 72-73 0.8139501616456385 74-75 0.5417989872113982 76-77 0.6342283013813355 78-79 0.3601589667163438 80-81 0.3099616888132895 82-83 0.29055250817331324 84-85 0.2820670874915146 86-87 0.2536848183144638 88-89 0.25674087020752545 90-91 0.26717696231042726 92-93 0.22400210152334435 94-95 0.1899953964154462 96-97 0.14574766634675654 98-99 0.15524093392945854 100-101 0.14945394204685253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1537932.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.157689765464156 #Duplication Level Percentage of deduplicated Percentage of total 1 87.6999322187303 43.98825992679309 2 6.895736466073379 6.917484207394134 3 1.8791427472135247 2.8276037681927404 4 0.8304294572533231 1.6660969235605985 5 0.49284657143392263 1.236002271597768 6 0.33037259609832614 0.9942435709266503 7 0.22688905497840597 0.796616158155036 8 0.16927487515766393 0.6792349338596626 9 0.1398075449459315 0.6311181119642296 >10 1.01063572406479 10.293132759678544 >50 0.15807138664624593 5.6081650686313145 >100 0.1485378259941205 14.87415240392761 >500 0.012735504100624462 4.322347861626594 >1k 0.005458073185981912 4.749876508393527 >5k 1.2995412347575983E-4 0.4156655252984798 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6377 0.4146477217458249 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 4012 0.26086979138219374 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3400 0.2210760943916896 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 3362 0.21860524392495898 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 3253 0.21151780442828422 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 2916 0.18960526213122555 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2854 0.1855738745276124 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2701 0.17562545027998636 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2499 0.16249092937789186 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 2472 0.16073532509889904 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2086 0.13563668614737193 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2056 0.1336860147262688 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2020 0.13134520902094501 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 1799 0.1169752628854852 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1716 0.11157840528709982 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 1716 0.11157840528709982 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 1666 0.10832728625192792 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 1605 0.10436092102901817 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 1604 0.10429589864831475 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 1573 0.10228020484650817 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1568 0.10195509294299097 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.502238070343812E-5 7 0.0 0.0 0.0 0.0 6.502238070343812E-5 8 0.0 0.0 0.0 0.0 6.502238070343812E-5 9 0.0 0.0 0.0 0.0 6.502238070343812E-5 10-11 0.0 0.0 0.0 0.0 6.502238070343812E-5 12-13 0.0 0.0 0.0 0.0 6.502238070343812E-5 14-15 0.0 0.0 0.0 0.0 6.502238070343812E-5 16-17 0.0 0.0 0.0 0.0 6.502238070343812E-5 18-19 0.0 0.0 0.0 0.0 6.502238070343812E-5 20-21 0.0 0.0 0.0 3.251119035171906E-5 6.502238070343812E-5 22-23 0.0 0.0 0.0 9.753357105515718E-5 6.502238070343812E-5 24-25 0.0 0.0 0.0 3.2511190351719064E-4 6.502238070343812E-5 26-27 0.0 0.0 0.0 9.103133298481337E-4 6.502238070343812E-5 28-29 0.0 0.0 0.0 0.0025033616570823676 6.502238070343812E-5 30-31 0.0 0.0 0.0 0.007347529019488508 6.502238070343812E-5 32-33 0.0 0.0 0.0 0.021229807299672547 6.502238070343812E-5 34-35 0.0 0.0 0.0 0.0434999726906001 6.502238070343812E-5 36-37 0.0 0.0 0.0 0.07971743874241513 6.502238070343812E-5 38-39 0.0 0.0 0.0 0.13212547758938625 6.502238070343812E-5 40-41 0.0 0.0 0.0 0.18872745999172916 6.502238070343812E-5 42-43 0.0 0.0 0.0 0.2457520878686444 6.502238070343812E-5 44-45 0.0 0.0 0.0 0.3130502518967029 6.502238070343812E-5 46-47 0.0 0.0 0.0 0.3953360746769038 6.502238070343812E-5 48-49 0.0 0.0 0.0 0.48093803887298003 6.502238070343812E-5 50-51 0.0 0.0 0.0 0.5706689242437246 6.502238070343812E-5 52-53 0.0 0.0 0.0 0.6555881534424148 6.502238070343812E-5 54-55 0.0 0.0 0.0 0.7508784523633034 6.502238070343812E-5 56-57 0.0 0.0 0.0 0.8771194044990285 6.502238070343812E-5 58-59 6.502238070343812E-5 0.0 0.0 1.0141215606411726 6.502238070343812E-5 60-61 6.502238070343812E-5 0.0 0.0 1.1549275260544678 6.502238070343812E-5 62-63 6.502238070343812E-5 0.0 0.0 1.2929375290975154 6.502238070343812E-5 64-65 6.502238070343812E-5 0.0 0.0 1.443886985900547 6.502238070343812E-5 66-67 6.502238070343812E-5 0.0 0.0 1.6164563842874717 6.502238070343812E-5 68-69 6.502238070343812E-5 0.0 0.0 1.807492138794173 6.502238070343812E-5 70-71 9.753357105515718E-5 0.0 0.0 2.022813752493608 6.502238070343812E-5 72-73 1.3004476140687625E-4 0.0 0.0 2.2454828952125325 6.502238070343812E-5 74-75 1.3004476140687625E-4 0.0 0.0 2.4686071945963803 6.502238070343812E-5 76-77 1.3004476140687625E-4 0.0 0.0 2.7152695957948723 6.502238070343812E-5 78-79 1.3004476140687625E-4 0.0 0.0 2.9903792885511193 6.502238070343812E-5 80-81 1.3004476140687625E-4 6.502238070343812E-5 0.0 3.2880842586018106 6.502238070343812E-5 82-83 1.3004476140687625E-4 6.502238070343812E-5 0.0 3.6019798014476585 6.502238070343812E-5 84-85 1.3004476140687625E-4 6.502238070343812E-5 0.0 3.921597313795408 6.502238070343812E-5 86-87 1.3004476140687625E-4 6.502238070343812E-5 0.0 4.260689029163839 9.753357105515718E-5 88-89 1.3004476140687625E-4 6.502238070343812E-5 0.0 4.623774003011837 1.3004476140687625E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2255 0.0 54.812897 2 ACCTGGG 2290 0.0 54.592003 3 GTACCTG 2490 0.0 50.40056 1 CCTGGGG 2185 0.0 45.94571 4 CTGGGGG 2015 0.0 41.126667 5 GTACACG 305 0.0 37.26492 1 ATAACGC 185 0.0 33.27608 3 TAACGCA 190 0.0 32.400394 4 TGGGGGG 4555 0.0 30.251787 6 TATCACG 110 2.79903E-6 30.136591 2 GTACTAG 1050 0.0 28.4145 1 CATGGGG 4045 0.0 26.457602 4 CTATTGA 1780 0.0 25.8055 9 ATGGGGG 3525 0.0 25.793047 5 TAGTACT 1225 0.0 25.126835 4 TACACGG 475 0.0 24.925001 2 AGGGCTA 1825 0.0 24.909723 5 GTACATG 6690 0.0 24.705185 1 GGGGGGA 4595 0.0 24.52663 7 TAGGCAT 1275 0.0 24.512877 5 >>END_MODULE