FastQCFastQC Report
Sat 18 Jun 2016
SRR1931084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537932
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71060.4620490372786313No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA45040.29286080268828535No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38880.2528070161749674No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA38460.25007607618542305No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC36340.23629133147629414No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC34680.2254976162795234No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33750.21945053487410368No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC33290.21645950536174552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29710.19318149306991467No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC28750.18693934452238462No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25230.16405146651477437No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT24160.1570940717795065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22040.14330932707037763No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT18330.11918602382940209No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA18140.11795059859603675No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT17810.1158048600328233No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC17460.11352907670820296No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC16860.10962773386599667No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA16470.10709186101856259No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC16060.10442594340972164No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15790.10267033913072879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG20750.055.166032
ACCTGGG21100.053.3505253
GTACCTG22550.050.8187071
CCTGGGG18150.046.8434874
CTGGGGG15850.045.8495375
TATCACG1350.045.7385522
GTACACG2600.034.7482871
TCACGCA1850.033.376784
GTACCCG1551.3424142E-930.6776031
TGGGGGG39650.029.9481226
GTACTAG10950.029.5289461
TAGTACT13650.028.1854614
CTGTGCG14600.027.3283489
TAACGCA1405.810689E-727.1415584
CAATGCG5550.026.9581718-19
TAGGCAT14650.025.9373255
CGACGAG5800.025.79617724-25
GGGGGGA41700.025.0587527
ATCACGC3050.024.9168383
GTACTGT16950.024.3793626
Produced by FastQC (version 0.11.5)