##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931084_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1537932 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.63909262568176 33.0 33.0 33.0 33.0 33.0 2 32.590942902547056 33.0 33.0 33.0 33.0 33.0 3 32.64482174764554 33.0 33.0 33.0 33.0 33.0 4 36.589176244463346 37.0 37.0 37.0 37.0 37.0 5 36.5398028001238 37.0 37.0 37.0 37.0 37.0 6 36.54219172239085 37.0 37.0 37.0 37.0 37.0 7 36.4825291365288 37.0 37.0 37.0 37.0 37.0 8 36.49346590096311 37.0 37.0 37.0 37.0 37.0 9 36.48435236408372 37.0 37.0 37.0 37.0 37.0 10-11 36.459379218326944 37.0 37.0 37.0 37.0 37.0 12-13 36.37172189667683 37.0 37.0 37.0 37.0 37.0 14-15 38.27507588111828 40.0 37.0 40.0 37.0 40.0 16-17 38.282871739452716 40.0 37.0 40.0 37.0 40.0 18-19 38.24844954133213 40.0 37.0 40.0 37.0 40.0 20-21 38.15650561923414 40.0 37.0 40.0 35.0 40.0 22-23 38.01416317496482 40.0 37.0 40.0 33.0 40.0 24-25 38.17330480151268 40.0 37.0 40.0 37.0 40.0 26-27 38.102167065904084 40.0 37.0 40.0 35.0 40.0 28-29 38.01426981166918 40.0 37.0 40.0 33.0 40.0 30-31 37.879308057833505 40.0 37.0 40.0 33.0 40.0 32-33 37.73408479698712 40.0 37.0 40.0 33.0 40.0 34-35 37.62682810423348 40.0 37.0 40.0 33.0 40.0 36-37 37.548416965119394 40.0 37.0 40.0 33.0 40.0 38-39 37.380030131371214 40.0 37.0 40.0 33.0 40.0 40-41 37.198985390771504 40.0 37.0 40.0 33.0 40.0 42-43 36.994011763849116 40.0 37.0 40.0 33.0 40.0 44-45 36.724199769560684 40.0 37.0 40.0 33.0 40.0 46-47 36.42598860027621 38.5 37.0 40.0 33.0 40.0 48-49 36.12441642413319 37.0 37.0 40.0 33.0 40.0 50-51 35.838528946663445 37.0 37.0 40.0 33.0 40.0 52-53 35.602518511871786 37.0 37.0 40.0 27.0 40.0 54-55 35.34887920922381 37.0 37.0 40.0 27.0 40.0 56-57 35.005331510105776 37.0 33.0 40.0 27.0 40.0 58-59 34.70595286397578 37.0 33.0 40.0 27.0 40.0 60-61 34.42361723405196 37.0 33.0 40.0 27.0 40.0 62-63 34.093177396659925 37.0 33.0 40.0 27.0 40.0 64-65 33.72759881451195 37.0 33.0 37.0 27.0 40.0 66-67 33.34707678883072 37.0 33.0 37.0 24.5 40.0 68-69 33.02116250913564 37.0 33.0 37.0 22.0 40.0 70-71 32.71878763170283 37.0 33.0 37.0 22.0 40.0 72-73 32.369633702920545 37.0 33.0 37.0 22.0 40.0 74-75 31.987461734328956 37.0 33.0 37.0 22.0 37.0 76-77 30.344208326505985 33.0 30.0 37.0 18.5 37.0 78-79 31.14440300351381 33.0 33.0 37.0 15.0 37.0 80-81 31.203418291575957 33.0 33.0 37.0 15.0 37.0 82-83 30.982648127485483 33.0 33.0 37.0 15.0 37.0 84-85 30.67978785798072 33.0 33.0 37.0 15.0 37.0 86-87 30.35825641185696 33.0 33.0 37.0 15.0 37.0 88-89 30.021533461817558 33.0 30.0 37.0 6.0 37.0 90-91 29.589138856594438 33.0 27.0 37.0 4.0 37.0 92-93 29.1887710249868 33.0 27.0 37.0 2.0 37.0 94-95 28.699451276129246 33.0 27.0 37.0 2.0 37.0 96-97 28.178267959831775 33.0 27.0 37.0 2.0 37.0 98-99 27.514926862826186 33.0 27.0 37.0 2.0 37.0 100-101 25.79545226967122 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09856714882397455 1101 2 0.169461957781877 1101 3 0.07382050005885787 1101 4 0.15938872538519888 1101 5 0.08831116646312154 1101 6 0.08131063508538716 1101 7 0.06850881775798712 1101 8 0.05539268486060678 1101 9 0.058775858320423424 1101 10-11 0.10150766367097219 1101 12-13 0.1348769668180978 1101 14-15 0.2399824401932733 1101 16-17 0.11256891170553018 1101 18-19 0.1296069721345532 1101 20-21 -0.00986800534924015 1101 22-23 0.08074735676791533 1101 24-25 0.21157850914357113 1101 26-27 0.22144282978479168 1101 28-29 0.21003476612629868 1101 30-31 0.0429430783230913 1101 32-33 0.11406558502523012 1101 34-35 0.08369368341845984 1101 36-37 0.04251157909025238 1101 38-39 -0.09242434644485797 1101 40-41 0.029057068759577476 1101 42-43 0.015065041835462978 1101 44-45 -0.0015716305141140197 1101 46-47 -0.18940552200614036 1101 48-49 -0.2548529935546 1101 50-51 -0.2450276222116088 1101 52-53 -0.2580215699338382 1101 54-55 -0.17054708273415287 1101 56-57 -0.22893686857788254 1101 58-59 -0.527573841575439 1101 60-61 -0.6646232760132875 1101 62-63 -0.6774263793514095 1101 64-65 -0.6207971862837738 1101 66-67 -0.8567279309546763 1101 68-69 -0.8805338256344228 1101 70-71 -1.062683907055515 1101 72-73 -1.1547261585031166 1101 74-75 -1.4067169058645206 1101 76-77 -1.1962277970847843 1101 78-79 -1.2302721657244504 1101 80-81 -1.2746779338043304 1101 82-83 -1.5836213296374595 1101 84-85 -1.6674439094169848 1101 86-87 -1.6995907740600096 1101 88-89 -1.9644413311748998 1101 90-91 -1.9523551505684154 1101 92-93 -2.0080579203904527 1101 94-95 -2.0257740172496703 1101 96-97 -2.552806186573928 1101 98-99 -2.7447241560368774 1101 100-101 -4.396817207294589 1102 1 0.12497324953685052 1102 2 0.22990357219465096 1102 3 0.14488462808245828 1102 4 0.1822860206264707 1102 5 0.146032878039712 1102 6 0.16550385384335442 1102 7 0.16096941413417198 1102 8 0.11931140776409421 1102 9 0.18352209380081064 1102 10-11 0.1369921822114435 1102 12-13 0.1845871406936439 1102 14-15 0.35620067843482417 1102 16-17 0.308193873027669 1102 18-19 0.29887438446849757 1102 20-21 0.15496535154719027 1102 22-23 0.21869362384466484 1102 24-25 0.32216977858752927 1102 26-27 0.21435704517634235 1102 28-29 0.22437915935803687 1102 30-31 0.29073573579294276 1102 32-33 0.3127525753264635 1102 34-35 0.2758921004812933 1102 36-37 0.28317694400048765 1102 38-39 0.19798112837843007 1102 40-41 0.236376455085896 1102 42-43 0.19070178638445867 1102 44-45 0.1042258479155791 1102 46-47 0.0693721518480288 1102 48-49 0.06224989232972433 1102 50-51 0.03931215734847626 1102 52-53 0.007210967671106516 1102 54-55 0.0011026344331739324 1102 56-57 -0.14301616436152642 1102 58-59 -0.27845796093519937 1102 60-61 -0.3238746939500601 1102 62-63 -0.24363080676557303 1102 64-65 -0.10501234136502546 1102 66-67 -0.39808091909949894 1102 68-69 -0.46887708481862234 1102 70-71 -0.5212011122270539 1102 72-73 -0.7069912096635491 1102 74-75 -0.7621223228282403 1102 76-77 -0.853284828003428 1102 78-79 -0.7148385032288687 1102 80-81 -0.7732784869418019 1102 82-83 -0.6703165973788394 1102 84-85 -0.7050994195849221 1102 86-87 -0.7250592982032984 1102 88-89 -0.7724356116307938 1102 90-91 -0.7938430772890186 1102 92-93 -0.9018436015057425 1102 94-95 -0.9109559805606686 1102 96-97 -1.0905205625635155 1102 98-99 -1.1638020153938484 1102 100-101 -1.9253719104976454 1103 1 0.1084790553786874 1103 2 0.21053215083690446 1103 3 0.052234721072601076 1103 4 0.04809347682630971 1103 5 0.1295602712981534 1103 6 0.1964372139576014 1103 7 0.1556349757753992 1103 8 0.1789790272492695 1103 9 0.0380182129933857 1103 10-11 0.16650476180917906 1103 12-13 0.21754220930174029 1103 14-15 0.25936891339602397 1103 16-17 0.2587204417406497 1103 18-19 0.17695968386015437 1103 20-21 0.09507951143767457 1103 22-23 0.08540657831257903 1103 24-25 0.13002722279696854 1103 26-27 0.1693041067300456 1103 28-29 0.06491549403057206 1103 30-31 0.16508406565429112 1103 32-33 0.1981398183834031 1103 34-35 0.1795246995452473 1103 36-37 0.22740890360761767 1103 38-39 0.14898825175662012 1103 40-41 -6.808327555489768E-4 1103 42-43 0.023292074581171107 1103 44-45 0.29784620315998467 1103 46-47 0.19663349239844052 1103 48-49 0.23046678579723334 1103 50-51 0.15782449338382776 1103 52-53 0.17941918675148116 1103 54-55 0.06755128383523612 1103 56-57 -0.037766480077493725 1103 58-59 0.03687318768900383 1103 60-61 -0.032108557004178806 1103 62-63 0.06700953701872692 1103 64-65 0.02013390165015494 1103 66-67 0.07722307630895386 1103 68-69 0.13768802400307578 1103 70-71 0.17700915014922458 1103 72-73 0.02814010518375909 1103 74-75 -0.03161963167518422 1103 76-77 -0.24419821506440087 1103 78-79 -0.0017021847938245571 1103 80-81 -0.061854613793219215 1103 82-83 -0.11508871169265689 1103 84-85 -0.20918296101000067 1103 86-87 -0.31706744886094285 1103 88-89 -0.14165223713275665 1103 90-91 -0.19290688734603378 1103 92-93 -0.3969864327705146 1103 94-95 -0.13978494298964605 1103 96-97 -0.35661996510829397 1103 98-99 -0.6048723570522618 1103 100-101 -1.2145548314722134 1104 1 0.13358790743291848 1104 2 0.22347155908585137 1104 3 0.11988166077548357 1104 4 0.1819862879842944 1104 5 0.1293822448371884 1104 6 0.13682327323952137 1104 7 0.1156631395615193 1104 8 0.2304544883044244 1104 9 0.088534556490103 1104 10-11 0.14089009207273762 1104 12-13 0.16352341771865042 1104 14-15 0.1867503622376674 1104 16-17 0.2127184042278074 1104 18-19 0.23271267570610377 1104 20-21 0.1837062019658262 1104 22-23 0.18177385936339618 1104 24-25 0.19706442187105466 1104 26-27 0.27260262550537817 1104 28-29 0.23915288430507076 1104 30-31 0.23154223366409354 1104 32-33 0.23832951447888462 1104 34-35 0.3523772664865348 1104 36-37 0.361855377059193 1104 38-39 0.22955835479388043 1104 40-41 0.3070761807081439 1104 42-43 0.3034897017554883 1104 44-45 0.19584104662354207 1104 46-47 0.029780194658528103 1104 48-49 0.15165358139659446 1104 50-51 0.12023442868456158 1104 52-53 0.11387006635796837 1104 54-55 0.049747302540474436 1104 56-57 0.054210223379818956 1104 58-59 0.07264626982106392 1104 60-61 -0.17922205821943749 1104 62-63 -0.19686350875142722 1104 64-65 0.044351731267255445 1104 66-67 0.04214273964311133 1104 68-69 -0.13006466341762035 1104 70-71 0.03230301175234018 1104 72-73 -0.08420104514311788 1104 74-75 -0.17683314548580853 1104 76-77 -0.09659291575073681 1104 78-79 0.16670706421490067 1104 80-81 -0.0679667072642296 1104 82-83 -0.13343077796567826 1104 84-85 -0.07412941732653522 1104 86-87 0.007376207321893702 1104 88-89 -0.05637756233146973 1104 90-91 -0.1844455121895372 1104 92-93 -0.07131239120935007 1104 94-95 -0.11468365706001293 1104 96-97 -0.17214182519997223 1104 98-99 -0.30788104874915234 1104 100-101 -0.8934353743817098 1105 1 0.04565649551781803 1105 2 0.18712390509356425 1105 3 0.08220656922040348 1105 4 0.08796644713283541 1105 5 0.07228676290960578 1105 6 0.09199526214747777 1105 7 0.09053032170585595 1105 8 0.09941078364001754 1105 9 0.010429569940200167 1105 10-11 0.018939017144710135 1105 12-13 0.07696580841042078 1105 14-15 0.19738277720438901 1105 16-17 0.14145956575896435 1105 18-19 0.18235663874448704 1105 20-21 0.09933240740404159 1105 22-23 0.07955333366424355 1105 24-25 0.07698509742032655 1105 26-27 0.19815857202340936 1105 28-29 0.25905936664634055 1105 30-31 0.2623273526444194 1105 32-33 0.21917905758207468 1105 34-35 0.22862091881560787 1105 36-37 0.30917727706906106 1105 38-39 0.28116857678294593 1105 40-41 0.0842409095700205 1105 42-43 0.2549186131338885 1105 44-45 0.3304260667764609 1105 46-47 0.3834866247491462 1105 48-49 0.26100119770404717 1105 50-51 0.2637454608127854 1105 52-53 0.12009420302602791 1105 54-55 0.34627441254163926 1105 56-57 0.3938883695232889 1105 58-59 0.37457945927456393 1105 60-61 0.3793350870149368 1105 62-63 0.15129922874239554 1105 64-65 0.35978731074350634 1105 66-67 0.43800134615970876 1105 68-69 0.31769315269141885 1105 70-71 0.14444692715540697 1105 72-73 0.13075238021741598 1105 74-75 0.5343928690723914 1105 76-77 0.45441561922819673 1105 78-79 0.560396464149612 1105 80-81 0.4073848731486329 1105 82-83 0.466209818843474 1105 84-85 0.6558471291593939 1105 86-87 0.6082847504729294 1105 88-89 0.5783467646661542 1105 90-91 0.6926142919424976 1105 92-93 0.8349154715992384 1105 94-95 0.7117434018337576 1105 96-97 0.748859126236141 1105 98-99 0.8635989022859718 1105 100-101 0.07212873577147505 1106 1 0.11606150176979213 1106 2 0.22220962681515743 1106 3 0.18139276966233098 1106 4 0.14214676262614034 1106 5 0.13617036187892495 1106 6 0.08731065952540007 1106 7 0.18291442241813627 1106 8 0.11394297818782206 1106 9 0.15417746891760942 1106 10-11 0.19435740737978335 1106 12-13 0.177674963488613 1106 14-15 0.29104938798281665 1106 16-17 0.29786302382317587 1106 18-19 0.1977769975803696 1106 20-21 0.26137953537394054 1106 22-23 0.2342607503678309 1106 24-25 0.26164882119560673 1106 26-27 0.336159574675186 1106 28-29 0.3361920244826635 1106 30-31 0.2132603943579099 1106 32-33 0.2538126684380728 1106 34-35 0.3344701239930217 1106 36-37 0.17400780639746216 1106 38-39 0.3008881596064015 1106 40-41 0.43233687218663164 1106 42-43 0.4553540826493574 1106 44-45 0.46361379872309527 1106 46-47 0.42995811632295045 1106 48-49 0.37108289463795785 1106 50-51 0.3768044579441323 1106 52-53 0.4657185706338751 1106 54-55 0.4571268667445665 1106 56-57 0.5703769779143713 1106 58-59 0.5168682163116856 1106 60-61 0.5574025382858139 1106 62-63 0.468724223800038 1106 64-65 0.5986290560916387 1106 66-67 0.5396633123748416 1106 68-69 0.3990691462717777 1106 70-71 0.6816105237223624 1106 72-73 0.813243552794475 1106 74-75 0.710269000705054 1106 76-77 0.5120622570498554 1106 78-79 0.6629115602315103 1106 80-81 0.7319018360646972 1106 82-83 0.6563530929436112 1106 84-85 0.8302862239033146 1106 86-87 0.9749391550921374 1106 88-89 0.9251652221339519 1106 90-91 1.0377917621403014 1106 92-93 0.9637641487578783 1106 94-95 1.1205262810125518 1106 96-97 1.3570122616917217 1106 98-99 1.2157065146818198 1106 100-101 0.996859784026384 1107 1 -0.010397940894307567 1107 2 0.07805734844776424 1107 3 0.025114109835442378 1107 4 -0.010556044361472061 1107 5 0.04233956096749836 1107 6 0.012090214940158717 1107 7 -0.00866664554499863 1107 8 -0.03955753154585295 1107 9 0.07210344177784833 1107 10-11 0.015569030119998217 1107 12-13 -0.02135816716973693 1107 14-15 0.057792006998518275 1107 16-17 -0.03655659056634164 1107 18-19 0.09104709144447298 1107 20-21 0.06120001163984057 1107 22-23 0.028269856526463855 1107 24-25 0.09613829549974895 1107 26-27 0.018211669051048318 1107 28-29 0.021955426822970026 1107 30-31 0.11101756616861991 1107 32-33 -0.0049561724153548425 1107 34-35 -0.01452340584162215 1107 36-37 0.002682458214827932 1107 38-39 -0.057073839849344665 1107 40-41 0.0641286165318391 1107 42-43 0.042260831848203395 1107 44-45 0.001550372419217183 1107 46-47 0.10144633558560656 1107 48-49 0.04437548989316298 1107 50-51 0.04102196597599317 1107 52-53 0.06155114241290249 1107 54-55 0.19204727924299192 1107 56-57 0.010682696273313752 1107 58-59 0.14975659885158166 1107 60-61 -0.0711543264662069 1107 62-63 -0.02442442753805807 1107 64-65 0.09453656481397843 1107 66-67 0.1390674722890708 1107 68-69 0.22886744607641418 1107 70-71 0.10035247663190461 1107 72-73 0.12997804856428985 1107 74-75 0.22426616569224223 1107 76-77 0.4054832402063049 1107 78-79 0.5715073759475544 1107 80-81 0.4228864546986095 1107 82-83 0.5251409421924151 1107 84-85 0.4999493528963974 1107 86-87 0.6749017881610619 1107 88-89 0.5448561732441952 1107 90-91 0.7086934830499416 1107 92-93 0.7418772812033403 1107 94-95 0.9534006509532915 1107 96-97 1.0269751384580204 1107 98-99 1.0445294157366227 1107 100-101 1.8068355361374095 1108 1 0.06713544702675023 1108 2 0.070870592558407 1108 3 0.16552878828552764 1108 4 0.10802354547768545 1108 5 0.16105091427240836 1108 6 0.12118848225058088 1108 7 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78-79 0.13753517307894825 2316 80-81 0.307087096971717 2316 82-83 0.3825461028007311 2316 84-85 0.4048526915953552 2316 86-87 0.48698865519771317 2316 88-89 0.48202632756592223 2316 90-91 0.48403383974375913 2316 92-93 0.5033308845796789 2316 94-95 0.5987549822925331 2316 96-97 0.787593466465772 2316 98-99 0.8610931745558652 2316 100-101 1.3547225216982817 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 12.0 5 29.0 6 70.0 7 158.0 8 411.0 9 753.0 10 1293.0 11 2034.0 12 3173.0 13 4546.0 14 5346.0 15 6580.0 16 8136.0 17 11007.0 18 11160.0 19 8618.0 20 7954.0 21 7831.0 22 8435.0 23 9354.0 24 10777.0 25 12464.0 26 15075.0 27 18621.0 28 22489.0 29 27966.0 30 34843.0 31 44557.0 32 58362.0 33 78803.0 34 112362.0 35 173214.0 36 276162.0 37 395462.0 38 159861.0 39 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.279797824011624 21.83354905800228 13.284214485634948 24.602438632351152 2 14.282974051467914 21.847497511263526 39.85216482685481 24.017363610413746 3 17.75624670011418 28.28258986743237 29.91049019072365 24.050673241729804 4 11.78777735296489 17.589919450274785 38.96316612177911 31.65913707498121 5 12.399117776338615 38.32432123136783 34.91877404202526 14.357786950268284 6 28.82292585107794 38.49825609974953 18.870990394893923 13.807827654278604 7 26.28893865268425 31.87358088654115 24.270643955649536 17.566836505125064 8 26.140102423254085 34.010801517882456 20.554614898448044 19.294481160415415 9 26.534528184601143 15.125181087330258 22.091678955896622 36.24861177217198 10-11 25.421962739574962 25.923317805988823 28.303169450925008 20.35155000351121 12-13 25.964867107258314 24.07369116449882 28.15790945243353 21.803532275809335 14-15 22.683839127657187 25.362053802926344 26.67333148019006 25.280775589226412 16-17 21.54191472704905 27.374194697814985 27.70613395130604 23.377756623829924 18-19 21.325975400732933 27.86456098188997 29.313584735866083 21.495878881511015 20-21 22.54790849010229 26.677902534052222 28.39221760129837 22.38197137454712 22-23 22.53103518230975 26.71548547009881 28.027507067932785 22.725972279658656 24-25 22.476806516803084 26.816952895186525 28.80631263280821 21.89992795520218 26-27 22.394618227593938 26.469343247945943 28.717687127909425 22.418351396550694 28-29 21.579205062382474 27.664259538133024 28.584261202705974 22.17227419677853 30-31 22.666769402028176 26.750142399013743 28.1961101010968 22.386978097861284 32-33 22.980729967254728 27.114105175001235 27.797295329052258 22.107869528691776 34-35 22.883976664768014 26.594186218896542 28.675292535690783 21.846544580644657 36-37 22.809623572433633 26.34258211676459 28.702992069870447 22.144802240931327 38-39 22.6296888481993 26.645668758871498 28.944829124323523 21.77981326860568 40-41 22.292961739537546 26.26716648893282 28.945366348479723 22.49450542304991 42-43 22.38181568715938 26.63872037954756 27.986495265084393 22.992968668208665 44-45 22.278293188515487 27.042905668130974 28.088790661745772 22.59001048160777 46-47 22.795318648678876 26.846733145548697 27.83998252198407 22.51796568378836 48-49 23.238378859896095 26.440182583082457 27.91264882016737 22.408789736854082 50-51 22.3324243204511 26.912795884343392 27.72108909886783 23.03369069633768 52-53 22.268799920932786 27.40813638054218 27.192912300413802 23.130151398111227 54-55 22.273286465201323 27.060851845205118 27.28322838721088 23.38263330238268 56-57 23.409324989661442 26.387740160163126 27.167065904084186 23.035868946091245 58-59 22.99776583099903 26.649032597019897 27.662666489805794 22.690535082175284 60-61 22.763035036659616 27.558110501634665 27.282870764117007 22.395983697588708 62-63 22.753834369790084 26.877261153288963 26.98432050311717 23.38458397380378 64-65 21.804865234613754 27.58489972248448 27.145803585594162 23.464431457307604 66-67 22.64910928313817 26.425776087439257 26.925486567476682 23.999628061945895 68-69 22.08356133695798 26.84882896362964 27.026842488755886 24.04076721065649 70-71 22.453503365288018 26.468538084552577 27.337608841106775 23.740349709052627 72-73 23.02289698114091 26.232661782185428 27.779804308643037 22.96463692803063 74-75 22.631697185472824 27.51409929505963 26.948762022235712 22.905441497231834 76-77 22.56647108681255 26.72248067257709 27.053060152265534 23.65798808834483 78-79 22.22394540438696 26.90498601133724 27.432864985426104 23.438203598849693 80-81 22.077964301935697 26.970996582612123 27.74576923089428 23.2052698845579 82-83 22.5890973737668 26.08377311966957 28.577842831764492 22.749286674799137 84-85 22.683018021386374 26.89809772448704 27.15321917296044 23.26566508116614 86-87 21.848768455449594 27.417955279397294 27.37975449411919 23.353521771033922 88-89 22.234208014398558 26.54928826502082 27.16371725147796 24.052786469102667 90-91 22.272571219013585 26.077388337065617 27.64657345058169 24.003466993339106 92-93 22.662774392791295 26.63083251810808 27.302779698664136 23.40361339043649 94-95 21.247355919132254 26.737908105306758 27.862386198044177 24.15234977751681 96-97 21.303203958178766 26.866320432231667 27.585882894558022 24.244592715031548 98-99 21.126226647211972 27.674110428809595 26.827356476099073 24.37230644787936 100-101 19.66077085533263 27.65383034836481 27.141030009207196 25.54436878709537 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 166.0 1 113.0 2 113.5 3 144.5 4 100.0 5 106.0 6 183.5 7 309.0 8 456.0 9 543.5 10 543.0 11 561.5 12 654.0 13 749.0 14 874.5 15 1051.5 16 1204.5 17 1358.0 18 1531.5 19 1721.0 20 1872.0 21 2028.5 22 2303.5 23 2657.5 24 3043.5 25 3578.0 26 4430.5 27 5565.0 28 7294.0 29 10761.0 30 15569.0 31 19809.5 32 25237.0 33 30865.5 34 35624.0 35 40370.0 36 43721.0 37 50116.5 38 52388.0 39 47734.0 40 43724.0 41 40330.0 42 39605.0 43 41471.0 44 45120.0 45 51716.0 46 57629.0 47 64733.0 48 76103.5 49 74865.5 50 65321.0 51 61720.5 52 58597.0 53 55991.5 54 53729.0 55 49044.5 56 43528.5 57 36372.0 58 30322.5 59 28234.0 60 23878.5 61 18236.5 62 14100.5 63 11101.0 64 8491.0 65 5830.5 66 4187.5 67 3204.5 68 2588.5 69 2137.5 70 1750.5 71 1337.0 72 1080.5 73 912.0 74 727.5 75 601.0 76 471.0 77 364.0 78 283.5 79 197.5 80 157.0 81 145.0 82 123.0 83 112.0 84 89.0 85 72.5 86 67.5 87 51.0 88 38.0 89 24.5 90 15.0 91 11.5 92 7.5 93 7.5 94 4.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0813429982600011 2 1.950671421103144E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.9260071316547157E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.502238070343812E-5 40-41 0.003381163796578782 42-43 0.00656726045104725 44-45 0.0 46-47 0.0 48-49 1.3004476140687625E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0026984287991926818 68-69 0.00829035353968836 70-71 0.001625559517585953 72-73 0.0 74-75 3.251119035171906E-5 76-77 0.0017230930886411103 78-79 0.005169279265923331 80-81 0.003998876413261444 82-83 0.00416143236502004 84-85 0.0024058280860272106 86-87 3.5762309386890966E-4 88-89 0.0 90-91 0.0 92-93 8.778021394964148E-4 94-95 0.004096409984316602 96-97 0.0034461861772822205 98-99 0.0 100-101 2.600895228137525E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1537932.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.93142892310078 #Duplication Level Percentage of deduplicated Percentage of total 1 83.70226895810052 35.93458010476957 2 9.225582168578917 7.921348502891434 3 2.534522623210363 3.2643203355703982 4 1.0991381600243142 1.8875028717500644 5 0.6542595588440177 1.404414887388561 6 0.412685891308941 1.0630317006177776 7 0.282642564986551 0.8493974432554103 8 0.2256714044594067 0.7750716688420278 9 0.1663892842337152 0.6428996756680821 >10 1.2688094821587041 11.096290660644573 >50 0.20299096204652406 6.155920954687055 >100 0.19924217515651446 17.40521901978728 >500 0.017450754073673556 5.030424395594911 >1k 0.008194267130246713 6.106646095676861 >5k 1.5174568759716134E-4 0.4629316828560736 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7106 0.4620490372786313 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 4504 0.29286080268828535 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3888 0.2528070161749674 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 3846 0.25007607618542305 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 3634 0.23629133147629414 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 3468 0.2254976162795234 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3375 0.21945053487410368 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 3329 0.21645950536174552 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2971 0.19318149306991467 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2875 0.18693934452238462 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2523 0.16405146651477437 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2416 0.1570940717795065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2204 0.14330932707037763 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 1833 0.11918602382940209 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 1814 0.11795059859603675 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1781 0.1158048600328233 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 1746 0.11352907670820296 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 1686 0.10962773386599667 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1647 0.10709186101856259 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 1606 0.10442594340972164 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1579 0.10267033913072879 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 6.502238070343812E-5 20-21 0.0 6.502238070343812E-5 0.0 3.251119035171906E-5 6.502238070343812E-5 22-23 0.0 6.502238070343812E-5 0.0 1.6255595175859532E-4 6.502238070343812E-5 24-25 0.0 6.502238070343812E-5 0.0 3.9013428422062874E-4 6.502238070343812E-5 26-27 0.0 6.502238070343812E-5 0.0 8.127797587929766E-4 6.502238070343812E-5 28-29 0.0 6.502238070343812E-5 0.0 0.0024058280860272106 6.502238070343812E-5 30-31 0.0 6.502238070343812E-5 0.0 0.007347529019488508 6.502238070343812E-5 32-33 0.0 6.502238070343812E-5 0.0 0.021197296109320828 9.753357105515718E-5 34-35 0.0 9.753357105515718E-5 0.0 0.0434999726906001 1.3004476140687625E-4 36-37 0.0 1.3004476140687625E-4 0.0 0.08004255064593233 1.3004476140687625E-4 38-39 0.0 1.3004476140687625E-4 0.0 0.13248310068325517 1.3004476140687625E-4 40-41 0.0 1.3004476140687625E-4 0.0 0.18869494880137744 1.3004476140687625E-4 42-43 0.0 1.3004476140687625E-4 0.0 0.2456220431072375 1.3004476140687625E-4 44-45 0.0 1.3004476140687625E-4 0.0 0.31184733785368923 1.6255595175859532E-4 46-47 0.0 1.3004476140687625E-4 0.0 0.3929302465908766 1.9506714211031437E-4 48-49 0.0 1.3004476140687625E-4 0.0 0.47758938626675307 1.9506714211031437E-4 50-51 0.0 1.3004476140687625E-4 0.0 0.5666050254497599 1.9506714211031437E-4 52-53 0.0 1.3004476140687625E-4 0.0 0.6509390532221191 1.9506714211031437E-4 54-55 0.0 1.3004476140687625E-4 0.0 0.7443762142929596 1.9506714211031437E-4 56-57 0.0 1.3004476140687625E-4 0.0 0.8679187376294921 1.9506714211031437E-4 58-59 0.0 1.3004476140687625E-4 0.0 1.0032628230636984 1.9506714211031437E-4 60-61 0.0 1.3004476140687625E-4 0.0 1.1423782065787043 1.9506714211031437E-4 62-63 0.0 1.3004476140687625E-4 0.0 1.27827498224889 1.9506714211031437E-4 64-65 0.0 1.3004476140687625E-4 0.0 1.4264934990623772 1.9506714211031437E-4 66-67 0.0 1.3004476140687625E-4 0.0 1.5967871141246817 1.9506714211031437E-4 68-69 0.0 1.3004476140687625E-4 0.0 1.7847668167383213 1.9506714211031437E-4 70-71 0.0 1.3004476140687625E-4 0.0 1.996024531643792 1.9506714211031437E-4 72-73 0.0 1.3004476140687625E-4 0.0 2.214369686045937 1.9506714211031437E-4 74-75 0.0 1.3004476140687625E-4 0.0 2.433365064255117 1.9506714211031437E-4 76-77 0.0 1.3004476140687625E-4 0.0 2.6762886785631617 1.9506714211031437E-4 78-79 0.0 1.3004476140687625E-4 0.0 2.9451237115815263 1.9506714211031437E-4 80-81 0.0 1.3004476140687625E-4 0.0 3.2348634399960465 1.9506714211031437E-4 82-83 0.0 1.3004476140687625E-4 0.0 3.5396883607337646 1.9506714211031437E-4 84-85 0.0 1.3004476140687625E-4 0.0 3.8514381650163987 1.9506714211031437E-4 86-87 0.0 1.3004476140687625E-4 0.0 4.180613967327554 1.9506714211031437E-4 88-89 0.0 1.3004476140687625E-4 6.502238070343812E-5 4.533035270740189 1.9506714211031437E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2075 0.0 55.16603 2 ACCTGGG 2110 0.0 53.350525 3 GTACCTG 2255 0.0 50.818707 1 CCTGGGG 1815 0.0 46.843487 4 CTGGGGG 1585 0.0 45.849537 5 TATCACG 135 0.0 45.738552 2 GTACACG 260 0.0 34.748287 1 TCACGCA 185 0.0 33.37678 4 GTACCCG 155 1.3424142E-9 30.677603 1 TGGGGGG 3965 0.0 29.948122 6 GTACTAG 1095 0.0 29.528946 1 TAGTACT 1365 0.0 28.185461 4 CTGTGCG 1460 0.0 27.328348 9 TAACGCA 140 5.810689E-7 27.141558 4 CAATGCG 555 0.0 26.95817 18-19 TAGGCAT 1465 0.0 25.937325 5 CGACGAG 580 0.0 25.796177 24-25 GGGGGGA 4170 0.0 25.058752 7 ATCACGC 305 0.0 24.916838 3 GTACTGT 1695 0.0 24.379362 6 >>END_MODULE