##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931083_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2945808 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.65291831646869 33.0 33.0 33.0 27.0 33.0 2 30.572842154003247 33.0 33.0 33.0 27.0 33.0 3 30.59462225644034 33.0 33.0 33.0 27.0 33.0 4 34.13272928853476 37.0 37.0 37.0 27.0 37.0 5 34.10607004937185 37.0 37.0 37.0 27.0 37.0 6 34.204002093822815 37.0 37.0 37.0 27.0 37.0 7 34.16154005963729 37.0 37.0 37.0 27.0 37.0 8 34.16185100997757 37.0 37.0 37.0 27.0 37.0 9 34.176804462476845 37.0 37.0 37.0 27.0 37.0 10-11 34.04960693297052 37.0 37.0 37.0 27.0 37.0 12-13 33.95859285465991 37.0 37.0 37.0 27.0 37.0 14-15 35.44960788347373 40.0 37.0 40.0 27.0 40.0 16-17 35.40275299680088 40.0 37.0 40.0 27.0 40.0 18-19 35.3275958582501 40.0 37.0 40.0 27.0 40.0 20-21 35.23312890724718 40.0 37.0 40.0 27.0 40.0 22-23 35.12732092519268 40.0 37.0 40.0 27.0 40.0 24-25 34.98450323306882 37.0 37.0 40.0 27.0 40.0 26-27 34.78121011281115 37.0 37.0 40.0 24.5 40.0 28-29 34.67264516221016 37.0 37.0 40.0 22.0 40.0 30-31 34.533368264326796 37.0 37.0 40.0 22.0 40.0 32-33 34.35226769701217 37.0 37.0 40.0 22.0 40.0 34-35 34.20093689065954 37.0 33.0 40.0 22.0 40.0 36-37 33.98115559466197 37.0 33.0 40.0 18.5 40.0 38-39 33.785926136394494 37.0 33.0 40.0 15.0 40.0 40-41 33.56882016750582 37.0 33.0 40.0 15.0 40.0 42-43 33.376330025582114 37.0 33.0 40.0 15.0 40.0 44-45 33.08188907763167 37.0 33.0 40.0 15.0 40.0 46-47 32.92992533797179 37.0 33.0 40.0 6.0 40.0 48-49 32.908738790851274 37.0 33.0 40.0 6.0 40.0 50-51 32.123536394768436 37.0 33.0 38.5 6.0 40.0 52-53 32.268036986796155 37.0 33.0 38.5 6.0 40.0 54-55 32.761986354847295 37.0 33.0 40.0 6.0 40.0 56-57 32.6824686130257 37.0 33.0 40.0 6.0 40.0 58-59 32.51537167391765 37.0 33.0 40.0 2.0 40.0 60-61 32.50492089097457 37.0 33.0 40.0 2.0 40.0 62-63 32.36029011395176 37.0 33.0 40.0 2.0 40.0 64-65 32.13271604938272 37.0 33.0 40.0 2.0 40.0 66-67 31.876209515351984 37.0 33.0 40.0 2.0 40.0 68-69 31.626953114391704 37.0 33.0 40.0 2.0 40.0 70-71 31.38787371749958 37.0 33.0 38.5 2.0 40.0 72-73 30.802764979930803 37.0 33.0 37.0 2.0 40.0 74-75 30.608256546251486 37.0 33.0 37.0 2.0 40.0 76-77 30.25452711106766 37.0 33.0 37.0 2.0 40.0 78-79 29.976701808128702 37.0 27.0 37.0 2.0 37.0 80-81 29.681077313932207 37.0 27.0 37.0 2.0 37.0 82-83 29.397288112463542 35.0 27.0 37.0 2.0 37.0 84-85 28.999703307208073 33.0 27.0 37.0 2.0 37.0 86-87 28.84081294503919 33.0 27.0 37.0 2.0 37.0 88-89 28.6217124469755 33.0 27.0 37.0 2.0 37.0 90-91 28.314467881138214 33.0 27.0 37.0 2.0 37.0 92-93 27.98823175169597 33.0 27.0 37.0 2.0 37.0 94-95 27.717905070527337 33.0 27.0 37.0 2.0 37.0 96-97 27.391975308641975 33.0 27.0 37.0 2.0 37.0 98-99 27.063111546984732 33.0 27.0 37.0 2.0 37.0 100-101 26.357624801073253 33.0 22.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4087465386864899 1101 2 0.3209717017135816 1101 3 0.404050910479377 1101 4 0.3504576303042981 1101 5 0.47162851986209375 1101 6 0.4021792644596047 1101 7 0.4173181531626966 1101 8 0.4321512391678439 1101 9 0.4530410395174371 1101 10-11 0.3726265874493535 1101 12-13 0.45685721450465877 1101 14-15 0.5782453795470843 1101 16-17 0.5746416443131324 1101 18-19 0.672729317286624 1101 20-21 0.7510623292730898 1101 22-23 0.600467481385607 1101 24-25 0.510742414883012 1101 26-27 0.5651086031175652 1101 28-29 0.6382590952011 1101 30-31 0.5618551025066054 1101 32-33 0.5745073951250816 1101 34-35 0.5216191455322914 1101 36-37 0.46900988247792696 1101 38-39 0.5535547247517698 1101 40-41 0.507436623260304 1101 42-43 0.4002265220081469 1101 44-45 0.3644012898853859 1101 46-47 0.23249838637547526 1101 48-49 0.24406497616865863 1101 50-51 0.4257080516199139 1101 52-53 0.5472641848900324 1101 54-55 0.6521027776873538 1101 56-57 0.5888251946099672 1101 58-59 0.6228872880189229 1101 60-61 0.5817317514323364 1101 62-63 0.6006692108483875 1101 64-65 0.5355899325525542 1101 66-67 0.6007402970132993 1101 68-69 0.5002248624766281 1101 70-71 0.4010351294781671 1101 72-73 0.3916274380915752 1101 74-75 0.352964978912933 1101 76-77 0.4609871835744812 1101 78-79 0.3781431908335513 1101 80-81 0.4416884026046688 1101 82-83 0.3928838884399326 1101 84-85 0.4118189559429055 1101 86-87 0.37261837099114103 1101 88-89 0.40484241151246536 1101 90-91 0.38082869739498193 1101 92-93 0.28248597503867146 1101 94-95 0.12366562901014788 1101 96-97 0.10078259094643016 1101 98-99 0.2677590671396928 1101 100-101 0.17629275471517403 1102 1 0.3136592109338956 1102 2 0.29755586894668085 1102 3 0.29971385625075087 1102 4 0.45026868975986645 1102 5 0.275306426758533 1102 6 0.320413589991297 1102 7 0.4391801516869336 1102 8 0.36220107323602946 1102 9 0.3613129132565902 1102 10-11 0.3944488931928092 1102 12-13 0.43621645961485456 1102 14-15 0.4598575171051067 1102 16-17 0.5335780086434241 1102 18-19 0.5747659349673526 1102 20-21 0.5831636839891488 1102 22-23 0.6141243423845424 1102 24-25 0.6078243412187305 1102 26-27 0.6586881140821461 1102 28-29 0.6562410880549479 1102 30-31 0.7475421474663122 1102 32-33 0.7449036323076257 1102 34-35 0.663471475302245 1102 36-37 0.6703221432580406 1102 38-39 0.6598824519038899 1102 40-41 0.8041927925434678 1102 42-43 0.746083242701097 1102 44-45 0.8459296800377913 1102 46-47 0.7330979945364859 1102 48-49 0.7320912780882338 1102 50-51 0.6294804651372701 1102 52-53 0.5941777803944248 1102 54-55 0.7853529694267465 1102 56-57 0.9077129970766009 1102 58-59 0.969150532576613 1102 60-61 1.002061361447133 1102 62-63 1.0531284548681157 1102 64-65 1.0032393711468828 1102 66-67 0.9969336430202169 1102 68-69 0.9862921875394264 1102 70-71 0.8800484076584034 1102 72-73 0.6523532489402051 1102 74-75 0.7510713570193843 1102 76-77 0.6844840012457638 1102 78-79 0.8158551128437779 1102 80-81 0.7282924525768628 1102 82-83 0.794192441652612 1102 84-85 0.8856694828173168 1102 86-87 0.8076197704161032 1102 88-89 0.6993112297798696 1102 90-91 0.8484292833360314 1102 92-93 0.7264998166237682 1102 94-95 0.6946260707195506 1102 96-97 0.7793866554581044 1102 98-99 0.9067356331325733 1102 100-101 0.9095842385781729 1103 1 0.12810440367908527 1103 2 0.17899680578604205 1103 3 0.1205623804435163 1103 4 0.1690118412362125 1103 5 0.04068569197665539 1103 6 0.07424913640561215 1103 7 0.15297262203245054 1103 8 0.16223917731906567 1103 9 0.09792644544402407 1103 10-11 -0.0216403658931128 1103 12-13 0.24565377439515146 1103 14-15 0.2584458883848839 1103 16-17 0.13456183489857665 1103 18-19 0.1466575380785926 1103 20-21 0.19007956149103933 1103 22-23 0.15324874095271213 1103 24-25 0.15969535618367559 1103 26-27 0.15159434967692675 1103 28-29 0.26039536942455754 1103 30-31 0.22569200697108016 1103 32-33 0.04026950817434738 1103 34-35 0.09516373218075813 1103 36-37 0.08006207882034744 1103 38-39 0.04414706684842429 1103 40-41 0.15228444697580557 1103 42-43 0.11972078086794369 1103 44-45 0.21784256064269414 1103 46-47 0.0524463061578615 1103 48-49 0.009542196351858934 1103 50-51 0.13204339895625594 1103 52-53 0.25858902820950647 1103 54-55 0.3706740906366832 1103 56-57 0.3896508068164479 1103 58-59 0.2793446367839536 1103 60-61 0.3868727726972523 1103 62-63 0.39227331674013755 1103 64-65 0.37351756216367704 1103 66-67 0.48025116523979605 1103 68-69 0.4253358320484182 1103 70-71 0.4338284941769288 1103 72-73 0.1966494490213826 1103 74-75 0.2803199724556933 1103 76-77 0.23367293928306765 1103 78-79 0.33005682212672127 1103 80-81 0.33103377538290246 1103 82-83 0.3456925253106178 1103 84-85 0.433839598007534 1103 86-87 0.4361057749682402 1103 88-89 0.4314554027116131 1103 90-91 0.42779942338617616 1103 92-93 0.26157267100632353 1103 94-95 0.19494184066696363 1103 96-97 0.3243745776307847 1103 98-99 0.36904115541566185 1103 100-101 0.364519036222827 1104 1 -0.007105493844974831 1104 2 0.008733347745469189 1104 3 -0.03710714941743731 1104 4 0.028655381604387742 1104 5 0.00592756543218087 1104 6 0.02005375658367825 1104 7 0.03830766334076685 1104 8 -0.0415378756720699 1104 9 3.2951304622486077E-4 1104 10-11 -0.023080190852958538 1104 12-13 -0.06696639752951938 1104 14-15 -0.009426715882206338 1104 16-17 -0.020589152324092197 1104 18-19 -0.02419122453905942 1104 20-21 -0.04914253400293944 1104 22-23 -0.022752914500685506 1104 24-25 0.02950898722578188 1104 26-27 0.0961592982138626 1104 28-29 0.0014043288754166383 1104 30-31 -0.025355057067493192 1104 32-33 -0.044550096772880465 1104 34-35 -0.03257993498102252 1104 36-37 0.06359464037613094 1104 38-39 0.09926033827959913 1104 40-41 0.15794405383632437 1104 42-43 -0.051616151217800166 1104 44-45 0.035302414235339086 1104 46-47 -0.030429445568124436 1104 48-49 -0.07873507316296013 1104 50-51 0.05607632590374578 1104 52-53 0.11432491075007789 1104 54-55 0.09342363194666348 1104 56-57 0.005810073096583324 1104 58-59 -0.02609565831237859 1104 60-61 0.04227008279012523 1104 62-63 -0.015226946080993287 1104 64-65 0.03339830659589893 1104 66-67 -0.032082499678740106 1104 68-69 -0.03325980018412267 1104 70-71 0.04207773966925643 1104 72-73 0.05943629174793941 1104 74-75 0.007030247393451816 1104 76-77 -0.09393871621380612 1104 78-79 0.07592861196803824 1104 80-81 0.1295082873605118 1104 82-83 0.009072883376589402 1104 84-85 0.1251054699099221 1104 86-87 0.10391572421242756 1104 88-89 -0.0060855711666505385 1104 90-91 0.0632340937266278 1104 92-93 -0.10477440687032313 1104 94-95 -0.1701690826396529 1104 96-97 -0.10204164458467346 1104 98-99 -0.09653771739758454 1104 100-101 0.19903182467701086 1105 1 -0.009056917280048538 1105 2 -0.07238770689390606 1105 3 -0.014503301603934204 1105 4 0.07080785962111236 1105 5 -0.15194458686784174 1105 6 -0.004043063652055423 1105 7 -0.10352856525743448 1105 8 0.06276983202957354 1105 9 -0.06659615679496511 1105 10-11 0.020899596893364958 1105 12-13 0.07412923785327763 1105 14-15 0.09870302046276436 1105 16-17 0.030450805073151344 1105 18-19 0.029775266684708868 1105 20-21 -0.003477349484825254 1105 22-23 -0.05795562633703355 1105 24-25 -0.1275276826014533 1105 26-27 0.13339160587730703 1105 28-29 0.008649827228019547 1105 30-31 -0.050866917863878314 1105 32-33 0.006794011361904495 1105 34-35 0.02169419467161049 1105 36-37 -0.008063465812291781 1105 38-39 -0.04460127686304105 1105 40-41 0.08744410170187678 1105 42-43 -0.10266981884995374 1105 44-45 -0.08495582886300213 1105 46-47 -0.37138795454880835 1105 48-49 -0.3324533129227376 1105 50-51 -0.20742885680557777 1105 52-53 -0.16630435535788735 1105 54-55 -0.018225545751931804 1105 56-57 -0.04043695454853946 1105 58-59 0.013459090985158184 1105 60-61 0.06549943000495517 1105 62-63 0.1814700659056001 1105 64-65 0.11382819702466662 1105 66-67 0.1284362090191813 1105 68-69 0.12573117944343082 1105 70-71 0.060668818202927355 1105 72-73 -0.10987572866443784 1105 74-75 -0.06965975553620751 1105 76-77 -0.09189625200834328 1105 78-79 -0.15709330660856224 1105 80-81 -0.012906537203804191 1105 82-83 0.05781926279218652 1105 84-85 0.07367513161348072 1105 86-87 -0.054122153558662234 1105 88-89 0.0034347437935267067 1105 90-91 -0.035475628850377916 1105 92-93 0.043657046044156544 1105 94-95 0.015542784025665668 1105 96-97 -0.08323108202727525 1105 98-99 0.02279556590541887 1105 100-101 0.0027336121549019765 1106 1 0.3118228168359636 1106 2 0.31504920393535585 1106 3 0.3643453585569105 1106 4 0.34043499335072624 1106 5 0.2898480815436031 1106 6 0.3454191993993234 1106 7 0.2410296074612006 1106 8 0.23372073461530363 1106 9 0.36184262242737475 1106 10-11 0.3608100976862829 1106 12-13 0.3842821352753205 1106 14-15 0.5082810956293784 1106 16-17 0.4826658574693923 1106 18-19 0.4656364051683042 1106 20-21 0.5923320108466115 1106 22-23 0.5523701592533783 1106 24-25 0.6057404865499265 1106 26-27 0.5718453604997222 1106 28-29 0.641517475065946 1106 30-31 0.5672323012255234 1106 32-33 0.6457547751073136 1106 34-35 0.5948921136561083 1106 36-37 0.637812471474696 1106 38-39 0.5987491510307592 1106 40-41 0.5732232993171138 1106 42-43 0.5364191540989651 1106 44-45 0.5608461144070773 1106 46-47 0.49749729644808127 1106 48-49 0.6511095961193547 1106 50-51 0.4688584448321791 1106 52-53 0.4898159564412481 1106 54-55 0.6396229413441503 1106 56-57 0.6524974115222903 1106 58-59 0.6651660746782184 1106 60-61 0.6104598900040301 1106 62-63 0.6886008640167276 1106 64-65 0.6270023107741309 1106 66-67 0.6847730283713496 1106 68-69 0.7705597217083238 1106 70-71 0.7975745261614371 1106 72-73 0.5725124591355559 1106 74-75 0.7042512609190723 1106 76-77 0.549830016044254 1106 78-79 0.6552054173036943 1106 80-81 0.7183371273896206 1106 82-83 0.7393952823732306 1106 84-85 0.8098094651905186 1106 86-87 0.7220584836728676 1106 88-89 0.6891558410947738 1106 90-91 0.6183775345262958 1106 92-93 0.7173287739722198 1106 94-95 0.886803613818234 1106 96-97 0.9576063748389814 1106 98-99 0.9252793984776204 1106 100-101 0.857492010210926 1107 1 0.01613893741700778 1107 2 0.09171728900623677 1107 3 0.09493032514097877 1107 4 0.23410473878888638 1107 5 0.08780724818940655 1107 6 0.1292527288061862 1107 7 0.18815316715515706 1107 8 0.10160389148246196 1107 9 0.04697190579950927 1107 10-11 0.08765715199447754 1107 12-13 0.13806134814442572 1107 14-15 0.16944435395416235 1107 16-17 0.15981369690604197 1107 18-19 0.15220495163773506 1107 20-21 0.24500570278590317 1107 22-23 0.03406940749914611 1107 24-25 0.02921090447856045 1107 26-27 0.08320093774852211 1107 28-29 0.02761043894670223 1107 30-31 0.0014892660073755337 1107 32-33 0.05083933702900367 1107 34-35 0.008768278624863513 1107 36-37 0.07452744732010075 1107 38-39 0.056613368029921673 1107 40-41 0.0035012231070865596 1107 42-43 0.05938228393301159 1107 44-45 0.1250898129377802 1107 46-47 0.11588815858753065 1107 48-49 -0.041845941080381976 1107 50-51 -0.01830583140620945 1107 52-53 0.03154791141943036 1107 54-55 0.09134046197358003 1107 56-57 0.06748910968587296 1107 58-59 0.07423134630261785 1107 60-61 0.12226518482351878 1107 62-63 0.2369342390678284 1107 64-65 0.13110984250049285 1107 66-67 0.10798729918642991 1107 68-69 0.18903151632338933 1107 70-71 0.1276204991450065 1107 72-73 0.07906798966044803 1107 74-75 0.208330424561197 1107 76-77 0.24014982447625144 1107 78-79 0.3143553760030997 1107 80-81 0.2891330190235344 1107 82-83 0.28057990019673085 1107 84-85 0.5081924843890384 1107 86-87 0.5066496077999219 1107 88-89 0.5374675229049899 1107 90-91 0.5803033937037867 1107 92-93 0.5691013620592926 1107 94-95 0.6268618518264617 1107 96-97 0.5830567181762376 1107 98-99 0.6742418775144436 1107 100-101 0.759070610663148 1108 1 -0.1327200794261998 1108 2 -0.04827511501779114 1108 3 -0.03268329970584816 1108 4 -0.023605535126918653 1108 5 -0.005570944398272104 1108 6 -0.08962972090630217 1108 7 -0.03423530661671492 1108 8 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-1.3229869691596647 2316 92-93 -1.3893589318049244 2316 94-95 -1.3466480782231542 2316 96-97 -1.307638945247831 2316 98-99 -1.2506612049762822 2316 100-101 -1.3067581753072979 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 112800.0 3 16701.0 4 12470.0 5 10351.0 6 10662.0 7 11143.0 8 11575.0 9 11756.0 10 11795.0 11 11809.0 12 12407.0 13 12618.0 14 13129.0 15 14895.0 16 17246.0 17 20425.0 18 17878.0 19 15194.0 20 15448.0 21 16419.0 22 18069.0 23 20638.0 24 23837.0 25 27303.0 26 31225.0 27 36814.0 28 43544.0 29 52077.0 30 63224.0 31 78796.0 32 99751.0 33 131268.0 34 177328.0 35 248760.0 36 368056.0 37 594437.0 38 553667.0 39 293.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.889822761159195 23.947967936712587 12.977599625772159 23.184609676356057 2 14.084689691227368 21.752489650150476 43.06501521712194 21.097805441500224 3 17.846524856015638 30.890798647297206 29.53351306908873 21.729163427598422 4 10.920989606477342 17.935980897399904 43.65523934771709 27.487790148405665 5 11.558207058234046 38.96989256729439 36.65126482177211 12.820635552699464 6 25.271436563414863 41.49075567721997 20.57591669246604 12.661891066899134 7 23.86839196580361 32.69653012008929 27.32391248852607 16.111165425581028 8 23.376608387240445 36.16678344277699 21.865613780667307 18.590994389315256 9 25.538324289974092 15.83786180226274 24.430444889823097 34.193369017940064 10-11 23.317171946910992 27.277070888033244 30.175444254249474 19.23031291080629 12-13 24.04296549789183 25.414933199085954 29.79612876681777 20.745972536204444 14-15 21.427143194681324 26.84202646844753 27.83678288594614 23.89404745092501 16-17 19.556605302845774 28.830873660009182 29.414235919858978 22.198285117286062 18-19 18.98740601529284 30.457698904026625 30.568148333226965 19.98674674745357 20-21 20.12729614421578 28.17578402937326 29.477786739665312 22.21913308674564 22-23 20.369810252399343 29.302283108742998 28.641411796016573 21.686494842841082 24-25 20.568312666677528 29.18392848413746 29.469605622633928 20.778153226551087 26-27 20.676313595454964 28.144281636820867 29.713749164915026 21.465655602809143 28-29 19.788798183724126 30.394156713540056 28.505099449794418 21.3119456529414 30-31 21.412902673901353 28.805967666596054 28.113746720763878 21.66738293873871 32-33 21.518595237707277 29.415121419997504 27.968659192995602 21.097624149299616 34-35 21.875101839631096 28.22794289376633 29.357208616447505 20.53974665015507 36-37 21.572638135275618 28.333788217018895 29.864930097277213 20.22864355042827 38-39 21.8028466213684 28.22531203662968 30.25943985487174 19.712401487130187 40-41 20.88913028718854 28.337107067689594 29.957668545047184 20.816094100074682 42-43 21.32328741779631 28.389719966426863 28.705338010036296 21.581654605740532 44-45 20.76753604296301 29.620050988020193 28.77771327895064 20.834699690066163 46-47 22.078630521299264 28.484304693483793 28.437518115691102 20.999546669525845 48-49 21.9859212538285 27.709257663784058 28.980552778404878 21.324268303982564 50-51 21.10307888403683 28.07688577961771 28.443764099643243 22.376271236702216 52-53 20.227482837107683 29.27733612563162 28.24216352249247 22.253017514768228 54-55 20.61671920180424 29.28385033710307 28.032305490703845 22.067124970388846 56-57 21.825596727225367 28.043136477119607 28.40701644473916 21.72425035091587 58-59 22.089397036306117 27.49057438295534 29.18066506568224 21.2393635150563 60-61 21.35221649597713 29.679384173062523 28.30425134976385 20.664147981196493 62-63 21.394586049918683 28.659705945024506 27.427226601511734 22.518481403545078 64-65 20.20280375804057 29.353956582799906 27.61253392713333 22.830705732026193 66-67 21.347712711033182 27.071210319499627 28.33283190653586 23.248245062931332 68-69 20.239545000603968 28.381574089669453 27.871094846549994 23.507786063176592 70-71 21.314923427521716 27.651850499431596 28.510148322752787 22.5230777502939 72-73 22.038156846040273 26.972615359707607 29.5745249990031 21.414702795249024 74-75 21.358439572978664 28.96308251612389 28.029551463075265 21.648926447822184 76-77 20.991743753664167 28.110526970313593 28.90866608632195 21.989063189700296 78-79 20.700863273420794 28.160926784779218 28.874875946393168 22.26333399540682 80-81 20.95764186645277 28.051973303698773 29.65837227068901 21.33201255915944 82-83 21.751452871040684 26.70853531839746 30.459435622649657 21.0805761879122 84-85 22.594368237830903 27.68416963916715 28.460582907401644 21.2608792156003 86-87 20.968358653319456 28.820132379649877 28.70593336297641 21.50557560405426 88-89 21.583143692098304 27.891207050791067 28.336374002862613 22.189275254248017 90-91 21.662655593256222 26.544887289716478 29.73862188015726 22.05383523687004 92-93 22.19723225478403 27.782192275089017 28.439365475977223 21.581209994149727 94-95 20.38723602684109 27.633592381804956 29.42280567555259 22.55636591580137 96-97 20.52472220749003 27.659223282486867 28.94272599071736 22.873328519305748 98-99 20.04299690392371 28.695248308310173 28.667639984740624 22.59411480302549 100-101 20.63519774984195 27.082439101425916 28.656485583182562 23.625877565549573 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 42.0 1 29.0 2 27.5 3 34.5 4 26.5 5 27.5 6 46.5 7 81.5 8 115.0 9 140.0 10 148.5 11 188.5 12 309.5 13 448.0 14 569.0 15 719.0 16 966.0 17 1149.0 18 1255.0 19 1457.5 20 1715.0 21 1938.0 22 2203.0 23 2502.5 24 2808.5 25 3326.5 26 4563.0 27 6628.5 28 12285.5 29 28138.0 30 49810.0 31 67647.5 32 88866.0 33 110623.5 34 136248.0 35 157639.5 36 165169.0 37 179487.0 38 171365.0 39 131176.5 40 98576.0 41 77412.0 42 65980.0 43 62488.0 44 60933.0 45 66610.0 46 74965.5 47 88484.5 48 111247.5 49 111894.5 50 92632.0 51 83640.5 52 78609.0 53 74576.5 54 71566.5 55 64179.5 56 57193.5 57 48134.5 58 38962.5 59 35915.0 60 30942.0 61 24077.5 62 18914.5 63 15980.0 64 13044.0 65 8971.5 66 6683.0 67 5448.5 68 4433.0 69 3689.0 70 3051.0 71 2506.5 72 2049.0 73 1706.5 74 1450.5 75 1189.0 76 993.0 77 863.0 78 729.0 79 573.0 80 479.5 81 420.5 82 336.0 83 273.0 84 229.5 85 184.5 86 146.5 87 115.0 88 99.5 89 81.5 90 63.0 91 54.5 92 38.0 93 24.5 94 21.5 95 16.0 96 11.0 97 10.5 98 6.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.0551889328836094E-4 2 0.0038359594379538653 3 6.789308739741355E-5 4 0.0010523428546599098 5 0.001934952990826286 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.6973271849353387E-5 12-13 8.486635924676693E-5 14-15 0.0038020128942551587 16-17 0.0042602912341877 18-19 0.003937799069049986 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.715723495896542E-4 42-43 1.6973271849353387E-5 44-45 6.110377865767219E-4 46-47 0.743174707923938 48-49 0.5311445959818155 50-51 0.40740944420002934 52-53 0.3433353429687203 54-55 0.33391517709232915 56-57 0.30044388500540425 58-59 0.3009361098890355 60-61 0.2771565560280914 62-63 0.2712328841526671 64-65 0.2679740159575913 66-67 0.2554816878764672 68-69 0.23433299115217288 70-71 0.24840383351528683 72-73 0.8235940699461745 74-75 0.5455718770537659 76-77 0.6386023800600718 78-79 0.35932416505081116 80-81 0.3104920619402215 82-83 0.29216092834291985 84-85 0.28263892283543257 86-87 0.25515919571132945 88-89 0.2574675606828415 90-91 0.2681607219479341 92-93 0.22501466490687785 94-95 0.1931218871019428 96-97 0.14950057844910464 98-99 0.1583945728981658 100-101 0.15287825954712594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2945808.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.304136760717498 #Duplication Level Percentage of deduplicated Percentage of total 1 83.77276086822374 25.386612021735356 2 8.453363684198305 5.123437783480562 3 2.668680136251937 2.426161434587667 4 1.2145957794471822 1.472291064374307 5 0.7122205251008792 1.0791614098223536 6 0.45583206666922893 0.8288158372958886 7 0.31695504879322517 0.6723534401940847 8 0.2414671574779334 0.5853963010746404 9 0.183105428617973 0.49939667554219647 >10 1.4169498241593712 8.770299300143215 >50 0.23031055450533616 4.941662502339028 >100 0.2658101032549375 17.048702221500776 >500 0.038101087274739605 7.846588899627465 >1k 0.027134305477559365 15.779071975595608 >5k 0.0019220133046604548 4.072418663025861 >10k+ 7.914172430954813E-4 3.4676304696610134 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 20881 0.7088377789726961 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 15809 0.5366609093328554 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15038 0.5104881241411525 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 13310 0.45182849662978714 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 13208 0.44836594917251904 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 11897 0.40386203038351454 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 11067 0.37568639911358787 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 8725 0.2961835937712166 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 8689 0.29496151819806316 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 8669 0.294282587324089 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8451 0.2868822407977709 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 7780 0.2641041099759387 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 7000 0.23762580589094742 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6991 0.23732028699765903 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 6979 0.23691292847327458 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 6879 0.23351827410340387 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 6785 0.23032729899572546 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 6464 0.21943045846844056 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 6294 0.21365954603966042 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 6137 0.20832993867896343 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 6060 0.20571605481416302 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 6052 0.2054444824645734 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5676 0.19268058203385965 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 5231 0.17757437008793514 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 4932 0.16742435352202178 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 4877 0.16555729361859292 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 4776 0.16212869270502353 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 4725 0.16039741897638948 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 4681 0.1589037710536464 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 4681 0.1589037710536464 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 4526 0.15364205678034684 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 4495 0.15258971392568696 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 4317 0.14654722914731713 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 4313 0.14641144297252232 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 4120 0.1398597600386719 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4094 0.13897714990250554 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 4013 0.13622747986291028 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 3966 0.13463199230907105 No Hit ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA 3863 0.13113549830810425 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 3843 0.13045656743413012 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 3697 0.12550037205411893 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 3693 0.12536458587932411 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 3636 0.12342963288849783 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 3628 0.12315806053890817 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3564 0.12098548174219095 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 3456 0.11731925502273059 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 3453 0.1172174153916345 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 3451 0.11714952230423706 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3428 0.11636875179916681 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 3350 0.11372092139066768 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 3345 0.11355118867217415 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 3089 0.10486087348530522 No Hit CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT 3064 0.10401220989283756 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 3059 0.10384247717434403 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 2957 0.10037992971707592 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 2946 0.10000651773639015 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.3946543698706774E-5 0.0 7 0.0 0.0 0.0 3.3946543698706774E-5 0.0 8 0.0 0.0 0.0 3.3946543698706774E-5 0.0 9 0.0 0.0 0.0 3.3946543698706774E-5 0.0 10-11 0.0 0.0 0.0 3.3946543698706774E-5 0.0 12-13 0.0 0.0 0.0 3.3946543698706774E-5 0.0 14-15 0.0 0.0 0.0 3.3946543698706774E-5 0.0 16-17 0.0 0.0 0.0 3.3946543698706774E-5 0.0 18-19 0.0 0.0 0.0 6.789308739741355E-5 0.0 20-21 0.0 0.0 0.0 1.357861747948271E-4 0.0 22-23 0.0 1.6973271849353387E-5 0.0 2.8854562143900754E-4 0.0 24-25 0.0 3.3946543698706774E-5 0.0 4.413050680831881E-4 0.0 26-27 0.0 3.3946543698706774E-5 0.0 9.165566798650829E-4 0.0 28-29 0.0 3.3946543698706774E-5 0.0 0.002070739165621113 0.0 30-31 0.0 3.3946543698706774E-5 0.0 0.006959041458234888 0.0 32-33 0.0 3.3946543698706774E-5 0.0 0.02452637782231564 0.0 34-35 0.0 3.3946543698706774E-5 0.0 0.054704855170465964 0.0 36-37 0.0 3.3946543698706774E-5 0.0 0.10397826334913884 0.0 38-39 0.0 3.3946543698706774E-5 0.0 0.17170161802805883 0.0 40-41 0.0 3.3946543698706774E-5 0.0 0.24446603444623682 0.0 42-43 0.0 3.3946543698706774E-5 0.0 0.32245821859401563 0.0 44-45 0.0 3.3946543698706774E-5 0.0 0.4188833759701922 0.0 46-47 0.0 3.3946543698706774E-5 0.0 0.5378999581778581 0.0 48-49 0.0 3.3946543698706774E-5 0.0 0.657340872181758 0.0 50-51 0.0 3.3946543698706774E-5 0.0 0.7813645695849831 0.0 52-53 0.0 3.3946543698706774E-5 0.0 0.9013316550162129 0.0 54-55 0.0 3.3946543698706774E-5 0.0 1.041972185559955 0.0 56-57 0.0 3.3946543698706774E-5 0.0 1.2133173648791775 0.0 58-59 0.0 3.3946543698706774E-5 0.0 1.4072709423017387 0.0 60-61 0.0 3.3946543698706774E-5 0.0 1.6088964386002074 0.0 62-63 0.0 3.3946543698706774E-5 0.0 1.806380456567434 0.0 64-65 0.0 3.3946543698706774E-5 0.0 2.016356802615785 0.0 66-67 0.0 3.3946543698706774E-5 0.0 2.2595158951296215 0.0 68-69 0.0 3.3946543698706774E-5 0.0 2.5286271203011195 0.0 70-71 0.0 3.3946543698706774E-5 0.0 2.8096026624953154 0.0 72-73 0.0 3.3946543698706774E-5 0.0 3.1215374525427317 0.0 74-75 0.0 3.3946543698706774E-5 0.0 3.4180605117509355 0.0 76-77 0.0 3.3946543698706774E-5 0.0 3.7490732593570253 0.0 78-79 0.0 3.3946543698706774E-5 0.0 4.119430051109916 0.0 80-81 0.0 3.3946543698706774E-5 0.0 4.546443624295948 0.0 82-83 0.0 3.3946543698706774E-5 0.0 4.995929809410525 0.0 84-85 0.0 3.3946543698706774E-5 0.0 5.417596801964011 0.0 86-87 0.0 3.3946543698706774E-5 0.0 5.873481910565793 0.0 88-89 0.0 3.3946543698706774E-5 0.0 6.370034978518627 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2170 0.0 55.85585 2 ACCTGGG 2285 0.0 53.25103 3 GTACCTG 2525 0.0 47.627834 1 CCTGGGG 2280 0.0 44.438564 4 CTGGGGG 2260 0.0 39.3843 5 GTACACG 360 0.0 36.825108 1 GTACTAG 1860 0.0 32.582584 1 CTGTGCG 2345 0.0 30.688416 9 TAGTACT 2390 0.0 30.111113 4 GCATCGT 1825 0.0 29.31496 8 ACTGTGC 2605 0.0 28.534197 8 TGGGGGG 6870 0.0 28.462107 6 TAGGCAT 2185 0.0 28.385307 5 TATTCTC 2560 0.0 28.111069 5 TCTCTGC 2695 0.0 27.405615 8 AGGGCTA 5355 0.0 26.965847 5 CTAGGCA 2455 0.0 26.035347 4 GCTATTG 5645 0.0 25.832148 8 CGTTAAC 405 0.0 25.71912 1 CATGGGG 6645 0.0 25.507572 4 >>END_MODULE