##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931082_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5681733 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.347735629252554 33.0 33.0 33.0 27.0 33.0 2 31.29233492668522 33.0 33.0 33.0 27.0 33.0 3 31.299504922177793 33.0 33.0 33.0 27.0 33.0 4 34.95333606841434 37.0 37.0 37.0 33.0 37.0 5 34.95501442957633 37.0 37.0 37.0 33.0 37.0 6 35.04236453912917 37.0 37.0 37.0 33.0 37.0 7 35.00908138414811 37.0 37.0 37.0 33.0 37.0 8 35.00857256051983 37.0 37.0 37.0 33.0 37.0 9 35.02101453904997 37.0 37.0 37.0 33.0 37.0 10-11 34.94072011127591 37.0 37.0 37.0 33.0 37.0 12-13 34.879390495822314 37.0 37.0 37.0 33.0 37.0 14-15 36.59662324857574 40.0 37.0 40.0 33.0 40.0 16-17 36.558099527028105 40.0 37.0 40.0 33.0 40.0 18-19 36.50029330839728 40.0 37.0 40.0 33.0 40.0 20-21 36.42561433281008 40.0 37.0 40.0 33.0 40.0 22-23 36.36423578862294 40.0 37.0 40.0 33.0 40.0 24-25 36.259582683663595 40.0 37.0 40.0 33.0 40.0 26-27 36.099570324758304 40.0 37.0 40.0 33.0 40.0 28-29 36.01755846323648 40.0 37.0 40.0 33.0 40.0 30-31 35.909438018294765 40.0 37.0 40.0 33.0 40.0 32-33 35.76689612130665 40.0 37.0 40.0 27.0 40.0 34-35 35.6862295359532 40.0 37.0 40.0 27.0 40.0 36-37 35.52085420064617 37.0 37.0 40.0 27.0 40.0 38-39 35.3837395210229 37.0 37.0 40.0 27.0 40.0 40-41 35.19534805665806 37.0 37.0 40.0 27.0 40.0 42-43 35.067111830140554 37.0 37.0 40.0 27.0 40.0 44-45 34.87749433139501 37.0 37.0 40.0 27.0 40.0 46-47 34.78933056164378 37.0 37.0 40.0 27.0 40.0 48-49 34.841973830871666 37.0 37.0 40.0 27.0 40.0 50-51 34.08455650767117 37.0 35.0 38.5 22.0 40.0 52-53 34.211000235315524 37.0 35.0 38.5 24.5 40.0 54-55 34.775925584676365 37.0 37.0 40.0 27.0 40.0 56-57 34.78426502970133 37.0 37.0 40.0 27.0 40.0 58-59 34.68293063049602 37.0 37.0 40.0 27.0 40.0 60-61 34.728870135221065 37.0 37.0 40.0 27.0 40.0 62-63 34.601312750880766 37.0 37.0 40.0 27.0 40.0 64-65 34.411791349575914 37.0 33.0 40.0 24.5 40.0 66-67 34.197311718097275 37.0 33.0 40.0 22.0 40.0 68-69 33.99729554345479 37.0 33.0 40.0 22.0 40.0 70-71 33.744686876345654 37.0 33.0 40.0 22.0 40.0 72-73 33.10376305961579 37.0 33.0 40.0 15.0 40.0 74-75 32.87224074063319 37.0 33.0 37.0 15.0 40.0 76-77 32.46143130977819 37.0 33.0 37.0 15.0 40.0 78-79 32.155059028644956 37.0 33.0 37.0 15.0 38.5 80-81 31.834798027292024 37.0 33.0 37.0 15.0 37.0 82-83 31.530313814464705 37.0 33.0 37.0 6.0 37.0 84-85 31.11232761201556 37.0 33.0 37.0 6.0 37.0 86-87 30.904449047500123 37.0 33.0 37.0 2.0 37.0 88-89 30.64486970788666 37.0 33.0 37.0 2.0 37.0 90-91 30.315152964773247 35.0 33.0 37.0 2.0 37.0 92-93 29.996185318810298 33.0 33.0 37.0 2.0 37.0 94-95 29.698608769542673 33.0 33.0 37.0 2.0 37.0 96-97 29.345771703809383 33.0 27.0 37.0 2.0 37.0 98-99 28.968824212612596 33.0 27.0 37.0 2.0 37.0 100-101 28.207868796368995 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08603824494829482 1101 2 0.09760313153682532 1101 3 0.17563526480092762 1101 4 0.10822309481115155 1101 5 0.14306324030470563 1101 6 0.14054432790017302 1101 7 0.24274467412365652 1101 8 0.16795336694885066 1101 9 0.13454698324136416 1101 10-11 0.20579075695124516 1101 12-13 0.1667283034712881 1101 14-15 0.34274508467546383 1101 16-17 0.32381964690667786 1101 18-19 0.3709659210419929 1101 20-21 0.32159911318823475 1101 22-23 0.4166477087367326 1101 24-25 0.3731581003178235 1101 26-27 0.36577115734461074 1101 28-29 0.30028285930471554 1101 30-31 0.3224654866603913 1101 32-33 0.28917646348237014 1101 34-35 0.3846624492122501 1101 36-37 0.31958828720958365 1101 38-39 0.2977142657172678 1101 40-41 0.3686743241386523 1101 42-43 0.2970762153417468 1101 44-45 0.3400411111735764 1101 46-47 0.2962514489595449 1101 48-49 0.31348195796170586 1101 50-51 0.271757719209198 1101 52-53 0.32777416865112485 1101 54-55 0.45801616996880057 1101 56-57 0.439522253929141 1101 58-59 0.4770616701097339 1101 60-61 0.5493162322270138 1101 62-63 0.5668599710453108 1101 64-65 0.6142994034788458 1101 66-67 0.6280468776542989 1101 68-69 0.4458971605849271 1101 70-71 0.4126747520110712 1101 72-73 0.4291648575731486 1101 74-75 0.39742142627306265 1101 76-77 0.2875914982613068 1101 78-79 0.2950518240168307 1101 80-81 0.4502644347345388 1101 82-83 0.37802207583520797 1101 84-85 0.40887027186071023 1101 86-87 0.3245786932620156 1101 88-89 0.37066072575798614 1101 90-91 0.2723428466775175 1101 92-93 0.2748563547396863 1101 94-95 0.13699578857484696 1101 96-97 -0.013994026224047218 1101 98-99 0.05027101626678032 1101 100-101 0.10977167032551804 1102 1 0.24718812221330566 1102 2 0.21967213629990212 1102 3 0.21154639928887775 1102 4 0.29673791477548406 1102 5 0.23142054301577986 1102 6 0.3252375522890105 1102 7 0.3459724637456105 1102 8 0.29440087837500073 1102 9 0.3029524559315462 1102 10-11 0.31980080679857537 1102 12-13 0.2699085759808355 1102 14-15 0.49717341849339647 1102 16-17 0.5430676401203769 1102 18-19 0.5402824235771817 1102 20-21 0.575117913314017 1102 22-23 0.5219378858263894 1102 24-25 0.5648743144333537 1102 26-27 0.5770161587791165 1102 28-29 0.5679064100492326 1102 30-31 0.5594564925002601 1102 32-33 0.5840064349251648 1102 34-35 0.5891589896110787 1102 36-37 0.6008185144637963 1102 38-39 0.6162824351575864 1102 40-41 0.57601158055148 1102 42-43 0.5934262298140638 1102 44-45 0.5954417951655202 1102 46-47 0.6722806847514349 1102 48-49 0.6921694756462529 1102 50-51 0.5584775330860268 1102 52-53 0.6594147871212286 1102 54-55 0.6929830696711505 1102 56-57 0.6772061408763221 1102 58-59 0.7103596573805007 1102 60-61 0.7055211049555226 1102 62-63 0.7789043173666954 1102 64-65 0.6666986249704152 1102 66-67 0.7401656981646099 1102 68-69 0.6862361923300924 1102 70-71 0.7079747172748725 1102 72-73 0.5968809591920277 1102 74-75 0.7007471546746302 1102 76-77 0.5877421139062591 1102 78-79 0.6339469410258616 1102 80-81 0.652931610180925 1102 82-83 0.5752307723530627 1102 84-85 0.6524091182408718 1102 86-87 0.5230566846123246 1102 88-89 0.5708526457516321 1102 90-91 0.4952680194228414 1102 92-93 0.4168982321894994 1102 94-95 0.4111532055288194 1102 96-97 0.45208617046433375 1102 98-99 0.3768827622775923 1102 100-101 0.4996600639044537 1103 1 0.24121622820553057 1103 2 0.18846836178687454 1103 3 0.1435793475797169 1103 4 0.24911217513714234 1103 5 0.2234997293193075 1103 6 0.28034441435193 1103 7 0.2541788555830351 1103 8 0.2991797133638059 1103 9 0.2362857974116892 1103 10-11 0.2751269401476222 1103 12-13 0.28300255044825917 1103 14-15 0.3655514602765777 1103 16-17 0.3561039596410396 1103 18-19 0.40447923933283647 1103 20-21 0.40706975200335194 1103 22-23 0.36864876358038856 1103 24-25 0.37840742618985246 1103 26-27 0.524271393896349 1103 28-29 0.4762165310953037 1103 30-31 0.4668275515744611 1103 32-33 0.46646468315491774 1103 34-35 0.4767858567673855 1103 36-37 0.4984337528690759 1103 38-39 0.40392173841340195 1103 40-41 0.4944028446009554 1103 42-43 0.3930509716315953 1103 44-45 0.4986719956364212 1103 46-47 0.3311613673625473 1103 48-49 0.4138436622222912 1103 50-51 0.37497420849665986 1103 52-53 0.43261974226938804 1103 54-55 0.5518423221088113 1103 56-57 0.5530390397812184 1103 58-59 0.47354173955586276 1103 60-61 0.4513837698458616 1103 62-63 0.5182409832325305 1103 64-65 0.6500212371069196 1103 66-67 0.6221177038634664 1103 68-69 0.6013125006114208 1103 70-71 0.583812626465523 1103 72-73 0.46240700783058486 1103 74-75 0.514966349706647 1103 76-77 0.52238723373722 1103 78-79 0.5901042318999927 1103 80-81 0.5687842072482887 1103 82-83 0.6261067821191268 1103 84-85 0.663145447757671 1103 86-87 0.5545343547463517 1103 88-89 0.4770406867453829 1103 90-91 0.5905234091664546 1103 92-93 0.40286173335762854 1103 94-95 0.4769243874894471 1103 96-97 0.48593197456183645 1103 98-99 0.5179387830565112 1103 100-101 0.4993253116419005 1104 1 0.005788217435394927 1104 2 0.06522587143312109 1104 3 0.02746365875974277 1104 4 0.0725961972303466 1104 5 0.0484889862279374 1104 6 -0.04581073091269161 1104 7 0.04208889609125066 1104 8 0.046802785617487075 1104 9 0.017277662712857023 1104 10-11 -0.05724635147547019 1104 12-13 -0.053974606584191065 1104 14-15 0.036560589650441955 1104 16-17 0.06138167230917446 1104 18-19 0.06786794841894306 1104 20-21 0.08471206415701715 1104 22-23 0.1032768648028437 1104 24-25 0.0495440828196152 1104 26-27 0.04046096964190582 1104 28-29 0.042009852047783625 1104 30-31 0.03640469448311734 1104 32-33 0.08535156366169616 1104 34-35 0.132897962052688 1104 36-37 0.1516247515107807 1104 38-39 0.13739274108189647 1104 40-41 0.21833435776912324 1104 42-43 0.04679313593628365 1104 44-45 0.059696285540617566 1104 46-47 -0.03453924747284276 1104 48-49 0.06227697391918241 1104 50-51 0.08450828888803841 1104 52-53 0.1345935035834458 1104 54-55 0.21279760831077255 1104 56-57 0.2858949816873917 1104 58-59 0.3129249358584403 1104 60-61 0.23270281111135205 1104 62-63 0.26330370635827904 1104 64-65 0.20307877349820558 1104 66-67 0.2649228001456052 1104 68-69 0.2305952753040046 1104 70-71 0.27420360737738037 1104 72-73 0.1511518917150383 1104 74-75 0.16632343503834335 1104 76-77 -0.009877800580568419 1104 78-79 0.1200055983010131 1104 80-81 0.1969848972101822 1104 82-83 0.09629282541603956 1104 84-85 0.2572791564230634 1104 86-87 0.23224281579475914 1104 88-89 0.1067603596745954 1104 90-91 0.1308249519645237 1104 92-93 0.1335382926835358 1104 94-95 0.1241881296312144 1104 96-97 0.10605235728634455 1104 98-99 -0.011067150435490447 1104 100-101 0.07198917759216172 1105 1 0.0620039727747681 1105 2 0.06586743466693434 1105 3 0.03505479148925161 1105 4 0.06779984813049111 1105 5 0.04190073284540574 1105 6 0.08039132656242742 1105 7 0.12010522170905347 1105 8 0.16049016680948114 1105 9 0.08832834481058427 1105 10-11 0.12985738888011156 1105 12-13 0.12333830574948479 1105 14-15 0.2250865317280457 1105 16-17 0.12334284305724452 1105 18-19 0.189580111563302 1105 20-21 0.2104525175842724 1105 22-23 0.1939752462184785 1105 24-25 0.1721424139165677 1105 26-27 0.23785383277508743 1105 28-29 0.1172355872596782 1105 30-31 0.14978736808258475 1105 32-33 0.10351171685413618 1105 34-35 0.10432917920577012 1105 36-37 0.1697807114794756 1105 38-39 0.2120520416521714 1105 40-41 0.1341214957541439 1105 42-43 0.11153895668994096 1105 44-45 0.14287237298331235 1105 46-47 0.14634174883712348 1105 48-49 0.21703069191090663 1105 50-51 0.20490343033945635 1105 52-53 0.18947756094674872 1105 54-55 0.2917032467989813 1105 56-57 0.3015786868199015 1105 58-59 0.3848394220487563 1105 60-61 0.3088489589364656 1105 62-63 0.28960675063872543 1105 64-65 0.33223181218767905 1105 66-67 0.3915424328192074 1105 68-69 0.40207820888334567 1105 70-71 0.4138879275160363 1105 72-73 0.4360174988938397 1105 74-75 0.42183053556389183 1105 76-77 0.46067458676148476 1105 78-79 0.4097779761384217 1105 80-81 0.37765972668574577 1105 82-83 0.384634265648625 1105 84-85 0.427598470411656 1105 86-87 0.4414902719056819 1105 88-89 0.3874903999295398 1105 90-91 0.35635679251020136 1105 92-93 0.3143361233891149 1105 94-95 0.3500784908075545 1105 96-97 0.32107807572534597 1105 98-99 0.36289118289784383 1105 100-101 0.4733589640173008 1106 1 0.136415122581532 1106 2 0.07987756589250594 1106 3 0.08192058004358671 1106 4 0.1280437945048689 1106 5 0.10893030772695056 1106 6 0.1345595398421935 1106 7 0.09903707611635326 1106 8 0.0997557621281544 1106 9 0.11955487268237164 1106 10-11 0.12789350866439264 1106 12-13 0.10792919474269524 1106 14-15 0.21736119929720843 1106 16-17 0.20738254643504916 1106 18-19 0.22363325682848512 1106 20-21 0.2252283587598498 1106 22-23 0.2115356733020377 1106 24-25 0.24426066431504267 1106 26-27 0.27555880449935444 1106 28-29 0.30419102023856226 1106 30-31 0.3517237504271762 1106 32-33 0.3129677775759845 1106 34-35 0.2794059412529677 1106 36-37 0.34722106225132876 1106 38-39 0.2777874425342901 1106 40-41 0.33829748758802936 1106 42-43 0.37013958115772994 1106 44-45 0.3782669776690071 1106 46-47 0.3611153383259804 1106 48-49 0.33676706715927907 1106 50-51 0.2594096695586572 1106 52-53 0.4047387429594522 1106 54-55 0.5217467521156962 1106 56-57 0.4744288036346731 1106 58-59 0.4937726138214984 1106 60-61 0.41650346558508744 1106 62-63 0.480611398665971 1106 64-65 0.507563151299891 1106 66-67 0.5892672057400148 1106 68-69 0.6381396540022237 1106 70-71 0.5463049835652996 1106 72-73 0.4055426932212285 1106 74-75 0.46365623044805915 1106 76-77 0.44470855857309743 1106 78-79 0.43702863936251646 1106 80-81 0.5630576065521247 1106 82-83 0.7077887013349944 1106 84-85 0.7374180079561476 1106 86-87 0.6683262836089945 1106 88-89 0.6601274253012761 1106 90-91 0.6427926317206669 1106 92-93 0.6025634181990434 1106 94-95 0.6217310514529828 1106 96-97 0.6333693511898772 1106 98-99 0.594584969880092 1106 100-101 0.6704151906816378 1107 1 0.01749500928022485 1107 2 -0.02734322443352255 1107 3 0.011854344760859448 1107 4 0.03459618173800294 1107 5 0.005514378184308555 1107 6 0.0017461259999933532 1107 7 -0.08303659191767565 1107 8 0.0038545145633861466 1107 9 0.021026423325110954 1107 10-11 0.008522146386582108 1107 12-13 -0.006505087776474738 1107 14-15 0.0613568652902714 1107 16-17 0.027738879348895296 1107 18-19 0.06118009596805507 1107 20-21 0.11452727919078853 1107 22-23 0.0316950574656687 1107 24-25 0.0442319507628568 1107 26-27 0.05478461251232858 1107 28-29 0.0340921009168369 1107 30-31 0.07630639554279384 1107 32-33 0.08441388937279015 1107 34-35 0.037765076448174284 1107 36-37 0.10832248033274539 1107 38-39 -0.049886686630813415 1107 40-41 0.07484056245401405 1107 42-43 0.005036807312620795 1107 44-45 -0.009795797870175704 1107 46-47 -0.1628495746712062 1107 48-49 -0.11285703488046295 1107 50-51 -0.10540735528373801 1107 52-53 -0.14424414436846433 1107 54-55 0.029039591021323474 1107 56-57 -0.006568572566806097 1107 58-59 0.030011057814192554 1107 60-61 0.005656272101624893 1107 62-63 0.05782696811697008 1107 64-65 0.06232208855056598 1107 66-67 0.024375649188172588 1107 68-69 0.01790568771027523 1107 70-71 0.00915143689959308 1107 72-73 -0.12524881008827293 1107 74-75 0.036255888406380166 1107 76-77 0.02259570750644002 1107 78-79 0.038065995214928705 1107 80-81 0.07492999233319253 1107 82-83 0.1748893737211752 1107 84-85 0.24074010796300271 1107 86-87 0.24533229958976221 1107 88-89 0.2551820768463102 1107 90-91 0.2252194335906026 1107 92-93 0.3690328083927774 1107 94-95 0.32171167799831935 1107 96-97 0.3312986856001565 1107 98-99 0.24249240989170673 1107 100-101 0.33948778328095486 1108 1 0.08017554710795949 1108 2 0.05202422408717666 1108 3 0.0781322574988117 1108 4 0.03642958140768826 1108 5 0.06198171553442933 1108 6 0.08469059103158116 1108 7 0.09613607933306412 1108 8 0.01876612708586123 1108 9 0.04549481748202311 1108 10-11 0.14385569245375507 1108 12-13 0.09245932162406945 1108 14-15 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-1.0191955153104644 2316 64-65 -1.097078189972116 2316 66-67 -1.0916035074509125 2316 68-69 -1.0751527010347246 2316 70-71 -0.9742643772635518 2316 72-73 -0.6945478280857103 2316 74-75 -0.9080798443306932 2316 76-77 -0.9169242929010295 2316 78-79 -0.9162270189675681 2316 80-81 -0.8435454058201195 2316 82-83 -0.8171307943431962 2316 84-85 -0.9036602894050816 2316 86-87 -0.9327582219802544 2316 88-89 -0.8872698395782095 2316 90-91 -0.8951116399823427 2316 92-93 -0.8877464727793125 2316 94-95 -0.8776279766369477 2316 96-97 -0.7980676095048551 2316 98-99 -0.8186156664172266 2316 100-101 -0.8890683151434118 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 136786.0 3 20142.0 4 14767.0 5 12600.0 6 12767.0 7 13581.0 8 13660.0 9 14222.0 10 14299.0 11 14513.0 12 14676.0 13 15624.0 14 16576.0 15 18234.0 16 20930.0 17 25244.0 18 22548.0 19 19310.0 20 20485.0 21 22512.0 22 25376.0 23 29705.0 24 34887.0 25 41114.0 26 47052.0 27 55794.0 28 66726.0 29 81362.0 30 100294.0 31 125319.0 32 163390.0 33 216671.0 34 298188.0 35 430907.0 36 679063.0 37 1360534.0 38 1461261.0 39 614.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.92913425046478 25.35073563357171 14.088580162877278 22.631549953086232 2 13.245700434251212 22.4221438548666 44.80304862937701 19.529107081505177 3 16.344733636997248 32.11675337749836 31.16089846032539 20.377614525178995 4 10.256854329999099 19.59638936762188 45.11804992340272 25.028706378976302 5 11.172365094076941 39.66375118672014 37.58575450056023 11.57812921864269 6 23.828258033244435 43.58971109694876 21.53489085108364 11.04714001872316 7 22.47397052976618 33.25381534119959 29.12115018428356 15.151063944750659 8 21.383440580541183 39.31020693862242 22.946625615811232 16.35972686502516 9 25.00856692843539 16.321375890067344 24.88508347717149 33.78497370432578 10-11 21.330774253616415 28.698406999276628 32.288114711540324 17.682704035566633 12-13 21.892166051991417 26.36306939448775 31.463543695741077 20.28122085777976 14-15 19.92824297731537 28.17976375924497 28.395180914592704 23.496812348846955 16-17 18.690921917308636 30.047651450400814 30.61825261939528 20.64317401289527 18-19 18.392712171154677 31.38002940230422 31.218831555422376 19.00842687111873 20-21 18.94453681649595 29.717323922120244 30.71587489239639 20.62226436898742 22-23 19.12154267016771 30.64015855725709 29.975660595103644 20.262638177471555 24-25 19.145482398677025 30.332616885132914 30.669424393829203 19.852476322360857 26-27 19.53115361105494 29.70455493068752 30.11866273899178 20.64562871926576 28-29 19.29806451658323 31.027293961191067 29.559731159489544 20.114910362736158 30-31 20.709781681046962 30.27234824304486 29.052236351127377 19.9656337247808 32-33 20.216437484830774 30.414531974663365 29.479104350732428 19.889926189773437 34-35 20.76860176287763 29.330575723991252 30.08971030493689 19.811112208194224 36-37 20.87869141334167 29.92183898821011 30.148433585316308 19.05103601313191 38-39 21.463856186131945 29.034530485681042 30.922440389226313 18.5791729389607 40-41 20.236317502770508 29.228628898588028 31.09605620339825 19.43899739524322 42-43 20.891779048028045 29.366896452798507 29.99678355149596 19.744540947677493 44-45 19.912850034949926 30.329062575544064 30.03018198512928 19.727905404376735 46-47 20.93914797626586 29.227051640956336 29.313480761718246 20.520319621059553 48-49 20.76401045945845 28.099265760456316 30.44953835084628 20.68718542923895 50-51 20.62239310861635 28.952347792890876 29.127663447671292 21.29759565082148 52-53 19.31357268618322 29.27631692241092 30.15474649133753 21.25536390006833 54-55 20.19878385774339 29.800288962266603 29.179669998097957 20.82125718189205 56-57 20.565366508130555 28.701474227826097 29.97059877358213 20.76256049046122 58-59 20.1348373918432 28.220322974173012 31.05290569062344 20.591933943360353 60-61 20.202012446470256 30.224672237890037 28.854856252058497 20.718459063581207 62-63 20.68428971694575 29.066207779027952 28.301912767224835 21.947589736801465 64-65 19.502174466871093 28.980305065131645 28.995024180205736 22.522496287791526 66-67 20.211585600264137 27.735685025608436 29.70116474342725 22.351564630700178 68-69 19.145900466381068 28.738147614385273 29.14255802019917 22.973393899034484 70-71 19.447543896967463 28.274588623221668 29.958130261233045 22.319737218577824 72-73 20.092879669051293 28.18770619960921 29.987071206858918 21.732342924480584 74-75 20.168755798740726 29.098982430230393 28.96712620050801 21.765135570520872 76-77 19.50031837489479 29.016189246040952 30.12070079854571 21.362791580518557 78-79 19.511642989824292 28.494935021426937 29.715960032725775 22.277461956022997 80-81 19.65692062108154 28.790101863912014 30.314493991860136 21.238483523146307 82-83 20.492030233293608 27.778023446310556 30.252120347453566 21.477825972942274 84-85 21.299229186454482 27.49636251342159 29.46734805296724 21.737060247156688 86-87 20.20180327520262 28.744106850792296 29.4318692262402 21.622220647764884 88-89 21.04874180207233 27.25860804464557 29.806936200340377 21.885713952941725 90-91 20.89392463436521 26.7925363794891 30.437363954437206 21.876175031708485 92-93 20.199628692959703 28.02807171546393 29.79070405798656 21.98159553358981 94-95 19.69924837223148 27.1766581847469 30.106773784924872 23.017319658096746 96-97 20.148244040796328 27.117444961542027 29.55755149756906 23.176759500092587 98-99 19.74243078511568 27.792140848529144 29.914693320013008 22.55073504634217 100-101 20.43052984186318 26.317584287414764 29.56325186383566 23.688634006886396 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 30.0 1 25.0 2 31.5 3 37.5 4 31.5 5 40.5 6 50.5 7 73.0 8 118.0 9 144.5 10 161.0 11 195.5 12 320.5 13 587.0 14 792.0 15 988.5 16 1372.5 17 1615.0 18 1700.5 19 1781.5 20 1900.0 21 2017.5 22 2175.0 23 2568.0 24 2964.0 25 3339.0 26 4196.5 27 6103.0 28 11523.0 29 26046.5 30 48402.0 31 77002.0 32 118021.5 33 176222.0 34 260470.0 35 331132.5 36 394686.0 37 478646.5 38 500143.0 39 432312.5 40 356744.0 41 296888.0 42 247535.0 43 213881.5 44 180472.5 45 151931.0 46 131759.5 47 120632.5 48 115379.0 49 106233.0 50 95119.5 51 89826.5 52 84672.0 53 79895.5 54 75873.5 55 68522.5 56 61192.0 57 52040.5 58 43260.0 59 39649.5 60 34499.0 61 27617.5 62 22132.5 63 18245.0 64 14823.5 65 10988.5 66 8430.0 67 6860.0 68 5743.5 69 4782.5 70 4041.0 71 3416.0 72 2847.0 73 2397.0 74 1990.5 75 1708.5 76 1513.0 77 1286.5 78 1115.5 79 970.5 80 797.5 81 681.0 82 595.0 83 505.0 84 418.5 85 354.5 86 303.5 87 250.5 88 210.5 89 170.5 90 136.5 91 111.5 92 75.5 93 66.5 94 63.5 95 51.5 96 39.0 97 18.5 98 6.0 99 5.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.632084436209868E-4 2 0.0040304604246626866 3 2.2880343022102588E-4 4 0.0012144182065577528 5 0.0018832282333576744 6 0.0 7 0.0 8 0.0 9 0.0 10-11 5.2800791589467514E-5 12-13 1.1440171511051294E-4 14-15 0.003493652376836434 16-17 0.004734470979188919 18-19 0.0036168542238785243 20-21 0.0 22-23 0.0 24-25 1.7600263863155837E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.2000329828944797E-4 42-43 8.800131931577918E-6 44-45 5.720085755525647E-4 46-47 0.7467791957137021 48-49 0.5382160689353055 50-51 0.416299041155225 52-53 0.3506764573414485 54-55 0.34148031947294955 56-57 0.30824222116737976 58-59 0.3080574183968166 60-61 0.2849658722083562 62-63 0.27910498434192527 64-65 0.2751185245769205 66-67 0.263097544358385 68-69 0.2416252224453349 70-71 0.2554414295779122 72-73 0.8276172076371768 74-75 0.550193048494183 76-77 0.6451992728274982 78-79 0.36523187555627834 80-81 0.31680474953680504 82-83 0.29777006416880203 84-85 0.2889963326330188 86-87 0.261847925624101 88-89 0.26500717298753745 90-91 0.2756641327566783 92-93 0.23072185898210984 94-95 0.1978269658218716 96-97 0.1535183015463768 98-99 0.16191362740910212 100-101 0.1576191630264921 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5681733.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.642022835380544 #Duplication Level Percentage of deduplicated Percentage of total 1 76.14545262024455 24.855416032404104 2 12.18349617237657 7.953879205469749 3 4.546637949480235 4.452343793134249 4 2.1891085026707073 2.858277189332118 5 1.2146118688750747 1.9823694179972213 6 0.7503989576332143 1.4696723946425483 7 0.5150783586655164 1.1769239681899084 8 0.3484916290869656 0.9100377371676559 9 0.26312304379645557 0.7729981563706865 >10 1.4519872059192434 8.982775744050029 >50 0.17099379875154383 3.9429492195511022 >100 0.17599676518681684 12.108751781369815 >500 0.02348013531817946 5.263733497124905 >1k 0.01782750995974619 11.132971542696698 >5k 0.002065382251434009 4.541062989475675 >10k+ 0.00125009978376269 7.595837331023647 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 34736 0.6113627655505811 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 30143 0.5305247536271064 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 29333 0.5162685398979502 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 27693 0.4874041071623746 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 24873 0.4377713630682751 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 21510 0.37858167569648205 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 21474 0.37794806619740845 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 21322 0.37527282609020873 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 21253 0.37405840788365097 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 19212 0.3381362693389499 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 17655 0.3107326585040163 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 17127 0.30143971918427 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 17083 0.30066530757429116 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 15605 0.27465211758454683 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 14753 0.2596566927731381 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 14102 0.2481989209982236 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 13168 0.23176027455003603 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 11860 0.20873912941702824 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 11791 0.20752471121047045 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 11397 0.20059020724838708 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 10951 0.1927404895654196 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 10853 0.1910156637068303 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 10242 0.18026190248644208 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 9736 0.1713561689716852 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 9623 0.1693673391551486 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 8902 0.15667754890981325 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 8841 0.15560393281416077 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 8617 0.15166147370881386 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 8536 0.1502358523358982 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 8030 0.14133011882114138 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 7939 0.13972849480959418 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 7873 0.1385668773946259 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 7656 0.13474762013632108 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 7357 0.1294851412412375 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 7300 0.1284819262010376 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 7233 0.12730270852220618 No Hit GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA 6756 0.11890738265948084 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 6668 0.11735855943952311 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 6639 0.1168481517874916 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 6548 0.11524652777594442 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 6545 0.11519372698435494 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 6332 0.11144487078150275 No Hit AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA 6282 0.11056485758834497 No Hit ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC 6230 0.10964964386746086 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 6209 0.10928003832633458 No Hit AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA 6127 0.1078368166895558 No Hit GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA 6090 0.10718560692661903 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 6014 0.10584798687301919 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 5930 0.10436956470851412 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 5908 0.10398235890352468 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 5829 0.10259193805833539 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 5791 0.10192312803153544 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 5713 0.10055030745020929 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 5707 0.10044470586703036 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 5683 0.10002229953431463 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.7600263863155837E-5 0.0 5 0.0 0.0 0.0 1.7600263863155837E-5 0.0 6 0.0 0.0 0.0 1.7600263863155837E-5 0.0 7 0.0 0.0 0.0 1.7600263863155837E-5 0.0 8 0.0 0.0 0.0 1.7600263863155837E-5 0.0 9 0.0 0.0 0.0 5.280079158946751E-5 0.0 10-11 0.0 0.0 0.0 6.160092352104542E-5 0.0 12-13 0.0 1.7600263863155837E-5 0.0 7.040105545262335E-5 0.0 14-15 0.0 1.7600263863155837E-5 0.0 8.800131931577918E-5 1.7600263863155837E-5 16-17 0.0 1.7600263863155837E-5 0.0 1.0560158317893501E-4 1.7600263863155837E-5 18-19 0.0 1.7600263863155837E-5 0.0 1.4080211090524667E-4 1.7600263863155837E-5 20-21 0.0 1.7600263863155837E-5 0.0 2.464036940841817E-4 3.5200527726311674E-5 22-23 0.0 1.7600263863155837E-5 0.0 7.040105545262334E-4 5.280079158946751E-5 24-25 0.0 1.7600263863155837E-5 0.0 0.0017952269140418954 7.040105545262335E-5 26-27 0.0 1.7600263863155837E-5 0.0 0.003942459105346907 7.040105545262335E-5 28-29 0.0 1.7600263863155837E-5 0.0 0.009724145784393599 7.040105545262335E-5 30-31 0.0 1.7600263863155837E-5 0.0 0.03808697099986923 7.040105545262335E-5 32-33 0.0 2.6400395794733757E-5 0.0 0.12974034506725324 7.040105545262335E-5 34-35 0.0 3.5200527726311674E-5 0.0 0.27288329106629966 8.800131931577918E-5 36-37 0.0 3.5200527726311674E-5 0.0 0.4925873848700739 8.800131931577918E-5 38-39 0.0 3.5200527726311674E-5 0.0 0.7934462953468598 8.800131931577918E-5 40-41 0.0 3.5200527726311674E-5 0.0 1.0972268496249296 8.800131931577918E-5 42-43 0.0 4.400065965788959E-5 0.0 1.4392175767499107 8.800131931577918E-5 44-45 0.0 5.280079158946751E-5 0.0 1.8131967834461773 8.800131931577918E-5 46-47 0.0 5.280079158946751E-5 0.0 2.26366673689172 8.800131931577918E-5 48-49 0.0 5.280079158946751E-5 0.0 2.7046149475872943 8.800131931577918E-5 50-51 0.0 6.160092352104542E-5 0.0 3.1362350184353964 1.0560158317893501E-4 52-53 0.0 7.040105545262335E-5 0.0 3.564493438885636 1.0560158317893501E-4 54-55 0.0 7.040105545262335E-5 0.0 3.999941567123974 1.0560158317893501E-4 56-57 0.0 7.040105545262335E-5 0.0 4.520460570744877 1.0560158317893501E-4 58-59 0.0 7.040105545262335E-5 0.0 5.1085029866767755 1.0560158317893501E-4 60-61 0.0 7.040105545262335E-5 0.0 5.685140431625351 1.0560158317893501E-4 62-63 0.0 7.040105545262335E-5 0.0 6.241792776957312 1.0560158317893501E-4 64-65 0.0 7.040105545262335E-5 0.0 6.820102246972887 1.0560158317893501E-4 66-67 0.0 7.040105545262335E-5 0.0 7.4216176648920325 1.0560158317893501E-4 68-69 0.0 7.040105545262335E-5 0.0 8.057893603940911 1.0560158317893501E-4 70-71 0.0 7.040105545262335E-5 0.0 8.70617292294446 1.0560158317893501E-4 72-73 0.0 7.040105545262335E-5 0.0 9.414715545415458 1.0560158317893501E-4 74-75 0.0 7.040105545262335E-5 0.0 10.064006879591139 1.0560158317893501E-4 76-77 0.0 7.040105545262335E-5 0.0 10.768501441373608 1.0560158317893501E-4 78-79 0.0 8.800131931577918E-5 0.0 11.530408063877694 1.0560158317893501E-4 80-81 0.0 8.800131931577918E-5 0.0 12.380544809127779 1.0560158317893501E-4 82-83 0.0 8.800131931577918E-5 0.0 13.275087020104605 1.0560158317893501E-4 84-85 0.0 8.800131931577918E-5 0.0 14.095514519953683 1.0560158317893501E-4 86-87 0.0 8.800131931577918E-5 0.0 14.97444529688389 1.1440171511051294E-4 88-89 0.0 8.800131931577918E-5 0.0 15.87468999335238 1.2320184704209085E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 4180 0.0 48.593746 3 TACCTGG 4355 0.0 45.55507 2 CCTGGGG 3720 0.0 42.129322 4 CTGGGGG 3575 0.0 37.47989 5 GTACCTG 5390 0.0 37.4224 1 GTACACG 515 0.0 30.34014 1 TATTCGC 1250 0.0 29.924307 9 TGGGGGG 9425 0.0 28.080881 6 TGAACCG 1000 0.0 26.514044 5 TATATCC 5360 0.0 26.323938 4 GCATCGT 1890 0.0 24.299313 8 GTACATG 14650 0.0 24.110825 1 CATGGGG 9290 0.0 23.852259 4 GTACTAG 2580 0.0 23.674501 1 ACATGGG 14320 0.0 23.640814 3 GGGGGGA 9850 0.0 23.55267 7 GTATCAA 11330 0.0 23.486443 1 ATGGGGG 8240 0.0 23.271036 5 ATATATC 6265 0.0 23.201534 3 ACCGTGT 1170 0.0 23.066042 8 >>END_MODULE