FastQCFastQC Report
Sat 18 Jun 2016
SRR1931081_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931081_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962568
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA60340.30745431495876835No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA59070.3009832016011675No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC50200.25578731539493155No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC39690.20223503083714808No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC38160.194439122618936No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC36610.18654130710375386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33880.17263096106733627No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT33150.1689113447279279No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC31950.16279690690972234No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT29860.15214759437634773No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC26900.13706531442477407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26860.1368614998308339No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC25990.13242853241263486No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA21760.11087513910346036No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA20940.10669693992768657No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC20940.10669693992768657No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC20580.10486260858222493No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA20090.10236587980645767No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT19970.10175443602463712No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG19910.10144871413372684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG25050.058.03982
GTACCTG27300.054.8175541
ACCTGGG27900.051.7701953
CCTGGGG27900.042.94384
CTGGGGG25100.039.432725
GTACCCG2400.033.5453951
GTACTAG14600.031.7883831
TAGGCAT17500.028.9554215
TAGTACT17850.028.1223624
CTGTGCG17500.027.3338439
TGGGGGG61050.026.9946526
GTATCAA74700.026.9440921
CTAGGCA20450.026.1679274
GCATCGT16200.026.017788
GAGTACC6950.025.212281
AGGGCTA27100.024.9891195
GCTATTG27450.024.4985968
CTATTGA27950.023.8921189
CTAGTAC22800.023.8862043
ATTTAGG29050.023.8013691
Produced by FastQC (version 0.11.5)