FastQCFastQC Report
Sat 18 Jun 2016
SRR1931081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962568
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA66790.3403194182316231No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA66260.3376188748619156No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC55580.28320037827988637No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC53100.2705638734555949No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49770.25359630851007453No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC45990.23433582938272712No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC41210.2099799854068751No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT37470.1909233208734678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37360.19036283074013233No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC32670.16646556960064568No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT30470.15525576693393553No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC28650.14598220290965713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27070.13793152644901985No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC26720.13614814875204323No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA23310.11877295461864251No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC21820.11118086099437063No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA21730.11072227815800524No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG21430.10919366870345383No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC20740.10567786695798566No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG20170.10277350899433803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG25750.054.7847332
ACCTGGG27200.051.1657143
GTACCTG28650.050.1104051
CCTGGGG24850.044.344724
CTGGGGG22150.043.102525
ATAACGC2200.038.862453
TAACGCA2350.036.3818664
TATTCGC1950.034.101529
GTACACG3400.033.55661
GTACCCG2400.031.6923451
TGGGGGG55650.031.1535856
TAGTACT19700.030.1386724
TATCACG1751.5279511E-1029.856232
CTGTGCG20500.028.499139
GTATAAC4000.026.1461851
GGGGGGA60150.025.7431877
ACTGTGC24850.025.0394748
GTACTGT24300.024.8243456
CTAGTAC25850.024.805823
GTACTAG15350.024.4660781
Produced by FastQC (version 0.11.5)