##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931080_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1145307 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.692549683185383 33.0 33.0 33.0 33.0 33.0 2 31.61751041423828 33.0 33.0 33.0 27.0 33.0 3 31.630246737337675 33.0 33.0 33.0 27.0 33.0 4 35.30951351908266 37.0 37.0 37.0 33.0 37.0 5 35.288070360174174 37.0 37.0 37.0 33.0 37.0 6 35.40028918010629 37.0 37.0 37.0 33.0 37.0 7 35.398528080243985 37.0 37.0 37.0 33.0 37.0 8 35.40167396165395 37.0 37.0 37.0 33.0 37.0 9 35.4298891039695 37.0 37.0 37.0 33.0 37.0 10-11 35.334158876179046 37.0 37.0 37.0 33.0 37.0 12-13 35.2703628808695 37.0 37.0 37.0 33.0 37.0 14-15 37.063864972448435 40.0 37.0 40.0 33.0 40.0 16-17 37.01995141913915 40.0 37.0 40.0 33.0 40.0 18-19 36.96120603471384 40.0 37.0 40.0 33.0 40.0 20-21 36.88238699318174 40.0 37.0 40.0 33.0 40.0 22-23 36.79978468655129 40.0 37.0 40.0 33.0 40.0 24-25 36.686999206326334 40.0 37.0 40.0 33.0 40.0 26-27 36.526023590181495 40.0 37.0 40.0 33.0 40.0 28-29 36.41509612706462 40.0 37.0 40.0 33.0 40.0 30-31 36.278920848296565 40.0 37.0 40.0 33.0 40.0 32-33 36.10373288559312 40.0 37.0 40.0 33.0 40.0 34-35 35.96949464204794 40.0 37.0 40.0 33.0 40.0 36-37 35.76026602474271 38.5 37.0 40.0 30.0 40.0 38-39 35.56981621521565 37.0 37.0 40.0 27.0 40.0 40-41 35.3356680785152 37.0 37.0 40.0 27.0 40.0 42-43 35.14676195989372 37.0 37.0 40.0 27.0 40.0 44-45 34.8597053017226 37.0 37.0 40.0 27.0 40.0 46-47 34.660506309661955 37.0 37.0 40.0 22.0 40.0 48-49 34.63980400015018 37.0 37.0 40.0 22.0 40.0 50-51 33.85286390461248 37.0 35.0 38.5 22.0 40.0 52-53 33.993173009507494 37.0 35.0 38.5 22.0 40.0 54-55 34.502326013898454 37.0 37.0 40.0 24.5 40.0 56-57 34.439714853746636 37.0 37.0 40.0 22.0 40.0 58-59 34.288946107899456 37.0 33.0 40.0 22.0 40.0 60-61 34.291423609564944 37.0 33.0 40.0 22.0 40.0 62-63 34.14001791659354 37.0 33.0 40.0 22.0 40.0 64-65 33.930330906909674 37.0 33.0 40.0 22.0 40.0 66-67 33.68854682630945 37.0 33.0 40.0 22.0 40.0 68-69 33.4661453217347 37.0 33.0 40.0 22.0 40.0 70-71 33.24737865044045 37.0 33.0 40.0 18.5 40.0 72-73 32.66248481848098 37.0 33.0 38.5 15.0 40.0 74-75 32.47908246435235 37.0 33.0 37.0 15.0 40.0 76-77 32.11745235120365 37.0 33.0 37.0 10.5 40.0 78-79 31.845998496472998 37.0 33.0 37.0 6.0 38.5 80-81 31.576755402699888 37.0 33.0 37.0 6.0 37.0 82-83 31.306498606923732 37.0 33.0 37.0 4.0 37.0 84-85 30.9210290341367 37.0 33.0 37.0 2.0 37.0 86-87 30.766278386493752 37.0 33.0 37.0 2.0 37.0 88-89 30.577545147283654 37.0 33.0 37.0 2.0 37.0 90-91 30.302842381998886 35.0 33.0 37.0 2.0 37.0 92-93 30.037365090757326 33.0 33.0 37.0 2.0 37.0 94-95 29.805957267352774 33.0 33.0 37.0 2.0 37.0 96-97 29.5248396281521 33.0 33.0 37.0 2.0 37.0 98-99 29.224627981842424 33.0 30.0 37.0 2.0 37.0 100-101 28.55219823156586 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07115165313976846 1101 2 0.06260250488342933 1101 3 -0.06809339757085198 1101 4 -0.10517580656922121 1101 5 -0.15176613997438437 1101 6 -0.1215362851213655 1101 7 0.004911938711352093 1101 8 0.044908082621930134 1101 9 0.05979978313963841 1101 10-11 -0.1063464789158175 1101 12-13 0.024435191259421174 1101 14-15 -0.051587378985246346 1101 16-17 0.1012822321117568 1101 18-19 0.09869499195237097 1101 20-21 0.015847379178339338 1101 22-23 0.08967623777269296 1101 24-25 -0.024888743656255485 1101 26-27 0.09583133496896323 1101 28-29 -0.0855725631679789 1101 30-31 -0.0832084428365718 1101 32-33 -0.018882189270833294 1101 34-35 0.12510075917992225 1101 36-37 0.041312925369808795 1101 38-39 -0.024755902665980045 1101 40-41 0.0984163780800813 1101 42-43 0.04444280483237151 1101 44-45 0.1105556340097209 1101 46-47 0.13787204144887966 1101 48-49 0.12470730392180229 1101 50-51 -0.03235153484214237 1101 52-53 0.08671270822004828 1101 54-55 0.3299058525714358 1101 56-57 0.29224590518207805 1101 58-59 0.19302308475144514 1101 60-61 0.3457790955699025 1101 62-63 0.38677794553748157 1101 64-65 0.3923443770860615 1101 66-67 0.33885016381140076 1101 68-69 0.32930746312411685 1101 70-71 0.30418313465995794 1101 72-73 0.17633658436461985 1101 74-75 0.2662731535110865 1101 76-77 0.18740801393425244 1101 78-79 0.21399047306685048 1101 80-81 0.412430673405904 1101 82-83 0.4115436061593307 1101 84-85 0.36497603904744835 1101 86-87 0.10334602360193657 1101 88-89 0.10387219001203007 1101 90-91 0.23096320938687853 1101 92-93 0.2921036505906258 1101 94-95 0.21742327573214126 1101 96-97 0.19632189326747707 1101 98-99 0.23286930858531107 1101 100-101 0.043615213838588573 1102 1 0.05063903407448578 1102 2 -0.005514404969581221 1102 3 -0.013432119849575486 1102 4 -0.019494630196199125 1102 5 -0.026604100613553783 1102 6 0.009000878087732644 1102 7 -0.1127755577423315 1102 8 -0.05663294625344406 1102 9 -0.06191851061316811 1102 10-11 0.02135945110645565 1102 12-13 -0.05658048818561667 1102 14-15 0.014145315356500987 1102 16-17 0.028599122178142977 1102 18-19 -0.011390294218969643 1102 20-21 0.11191907950409075 1102 22-23 0.08134003101410059 1102 24-25 0.08082311653377872 1102 26-27 0.10336007285874871 1102 28-29 -0.08019562069613784 1102 30-31 -0.024824078925632875 1102 32-33 -0.09946030788292148 1102 34-35 -0.12448022994761487 1102 36-37 -0.10629674854370563 1102 38-39 -0.012606042263527684 1102 40-41 -0.07138450446156952 1102 42-43 -0.14591907216174604 1102 44-45 -0.1548237381432216 1102 46-47 -0.4990172101948005 1102 48-49 -0.44721380273246325 1102 50-51 -0.42424636602294896 1102 52-53 -0.3468453342426301 1102 54-55 -0.34839564030028924 1102 56-57 -0.29563362337248833 1102 58-59 -0.43808730860956757 1102 60-61 -0.3519787597927788 1102 62-63 -0.45138419712117894 1102 64-65 -0.32927854804630385 1102 66-67 -0.2988389184820406 1102 68-69 -0.39074926338253846 1102 70-71 -0.24227214975093858 1102 72-73 -0.4291293979176203 1102 74-75 -0.4250354989179428 1102 76-77 -0.5361226473318297 1102 78-79 -0.46142668280955945 1102 80-81 -0.43202281565980627 1102 82-83 -0.4324890534103396 1102 84-85 -0.41908132470490855 1102 86-87 -0.36985546679645864 1102 88-89 -0.2981491009779269 1102 90-91 -0.3428427187440306 1102 92-93 -0.2017646781525002 1102 94-95 -0.26768146971148 1102 96-97 -0.17202139884857104 1102 98-99 -0.14250005386345777 1102 100-101 -0.11580896628952786 1103 1 -0.023604291621428075 1103 2 -0.17874883159337784 1103 3 -0.05468896082665964 1103 4 -0.06265027745706675 1103 5 -0.17199358797805075 1103 6 -0.1530453599550583 1103 7 -0.06571258910638988 1103 8 -0.16863087022524326 1103 9 -0.04134388963600344 1103 10-11 -0.009945535158848884 1103 12-13 -0.0043052963006928735 1103 14-15 -0.06371312652525774 1103 16-17 -0.05418250244051137 1103 18-19 -0.04910797126155586 1103 20-21 -0.0569850139002952 1103 22-23 -0.15589999075887562 1103 24-25 -0.15195893004182892 1103 26-27 -0.017111801600620424 1103 28-29 -0.16735399379891902 1103 30-31 -0.1142093298423319 1103 32-33 -0.2288280897818069 1103 34-35 -0.18131255691361758 1103 36-37 -0.1793628143436763 1103 38-39 -0.2901693252573949 1103 40-41 -0.22591252451296384 1103 42-43 -0.14240628053675408 1103 44-45 -0.17387087495432496 1103 46-47 -0.3056955228733358 1103 48-49 -0.07308461639205888 1103 50-51 -0.2592749114107491 1103 52-53 -0.09406885749879024 1103 54-55 0.02935921114976736 1103 56-57 0.07312510586195486 1103 58-59 0.060617283547948375 1103 60-61 0.02438996307011365 1103 62-63 -0.024969269972039854 1103 64-65 -0.11508581498893022 1103 66-67 -0.10455622420251132 1103 68-69 -0.07941993020111227 1103 70-71 -0.25758813221441557 1103 72-73 -0.3755482492664015 1103 74-75 -0.221786219409438 1103 76-77 -0.16955176489325652 1103 78-79 -0.31445696666000345 1103 80-81 -0.3881888725986755 1103 82-83 -0.0893473307288879 1103 84-85 0.028351278220938525 1103 86-87 0.10686938773388022 1103 88-89 -0.10777267318108841 1103 90-91 -0.13599414643537244 1103 92-93 -0.1048685934910587 1103 94-95 -0.09535847010389986 1103 96-97 8.775719984228658E-4 1103 98-99 -0.08191231002508559 1103 100-101 -0.17249255342996506 1104 1 0.0056614862416068945 1104 2 -0.011904324271934996 1104 3 -0.055660090694296116 1104 4 0.018491019940469755 1104 5 -0.03574766432564758 1104 6 -0.07510119792424064 1104 7 -0.11671688925346047 1104 8 -0.13208361656452183 1104 9 -0.08578207302368668 1104 10-11 -0.1501169415786947 1104 12-13 -0.04410163877783191 1104 14-15 -0.029650646507349165 1104 16-17 0.05116110968302934 1104 18-19 0.07924202713987683 1104 20-21 -0.029474663174546833 1104 22-23 -0.0803237071090308 1104 24-25 -0.01399254792021054 1104 26-27 -0.1915584973612141 1104 28-29 -0.13625485862403508 1104 30-31 -0.23815627338092327 1104 32-33 -0.06040899329226335 1104 34-35 0.13231643151274852 1104 36-37 0.0703554381021334 1104 38-39 0.2095219513587594 1104 40-41 0.12667655567118885 1104 42-43 0.14963604953550913 1104 44-45 -0.054789571801947545 1104 46-47 -0.2531436093878199 1104 48-49 -0.24904495592783604 1104 50-51 -0.16163939283539008 1104 52-53 0.022143016661964054 1104 54-55 0.04625407335323928 1104 56-57 -0.0511775189861865 1104 58-59 0.15603079304344192 1104 60-61 0.17182785871560924 1104 62-63 0.24338428524248457 1104 64-65 0.12457045299495917 1104 66-67 0.1412235571134275 1104 68-69 0.0888343139960881 1104 70-71 0.23599561674651426 1104 72-73 0.18649316162943563 1104 74-75 0.15271123087510574 1104 76-77 0.06594522979949602 1104 78-79 0.31652667199976037 1104 80-81 0.33235551962589227 1104 82-83 0.35317816642566413 1104 84-85 0.26678763924146764 1104 86-87 0.2359137639727713 1104 88-89 0.3215856217866211 1104 90-91 0.42901560482749446 1104 92-93 0.1923461021416557 1104 94-95 0.14568855176451123 1104 96-97 0.1333179054596414 1104 98-99 0.16805571862125035 1104 100-101 0.32510360041618824 1105 1 -0.13444353285277444 1105 2 -0.13286381690696203 1105 3 -0.14441486428949446 1105 4 -0.13264441377475578 1105 5 -0.3042964913851094 1105 6 -0.19814061418173878 1105 7 -0.12280322313235104 1105 8 -0.31180947581165697 1105 9 -0.20496851601332366 1105 10-11 -0.14282868462288434 1105 12-13 -0.23484086409153093 1105 14-15 -0.16483715769120266 1105 16-17 -0.17444240913858522 1105 18-19 -0.334142792827393 1105 20-21 -0.12045427310362555 1105 22-23 -0.1515525669017137 1105 24-25 -0.08987566303205341 1105 26-27 -0.1340963451517183 1105 28-29 -0.22675710903319413 1105 30-31 -0.09694309825817271 1105 32-33 -0.2302637396097751 1105 34-35 -0.16223144082767504 1105 36-37 -0.17836592032667653 1105 38-39 -0.09057522540065577 1105 40-41 -0.04141366527807122 1105 42-43 -0.15341905139193557 1105 44-45 0.0018457255248378601 1105 46-47 5.299454016238769E-5 1105 48-49 0.13906538231548637 1105 50-51 0.03212595086346681 1105 52-53 -0.08918404280338876 1105 54-55 0.07392387695388436 1105 56-57 -0.029338320950181185 1105 58-59 0.12693743516018685 1105 60-61 0.00790600928844043 1105 62-63 0.06555081552071584 1105 64-65 0.23449100673200007 1105 66-67 0.15786294609323903 1105 68-69 0.24026809741125277 1105 70-71 0.09778298717750289 1105 72-73 0.11612525382801664 1105 74-75 -0.050635400330570235 1105 76-77 -0.09216306261730267 1105 78-79 -0.16331341256106668 1105 80-81 -0.1422250859387546 1105 82-83 0.023476175154520718 1105 84-85 0.07183742101997126 1105 86-87 0.010804653225360283 1105 88-89 -0.09236180047197351 1105 90-91 0.017633989988432575 1105 92-93 -0.043256393766693435 1105 94-95 -0.15260147824094972 1105 96-97 -0.24107687086332774 1105 98-99 -0.3619497369161202 1105 100-101 -0.41826451196788383 1106 1 -0.03589411162587197 1106 2 0.09455511974939057 1106 3 0.07316384939202081 1106 4 -0.006666063075527973 1106 5 0.023721744153668567 1106 6 -0.05151494506818466 1106 7 -0.035787402073971464 1106 8 0.09549635297089765 1106 9 0.06155068909868078 1106 10-11 -0.01100723733904374 1106 12-13 0.02231750313635672 1106 14-15 -0.01981709103641549 1106 16-17 0.0246077331915302 1106 18-19 0.0041396851749126995 1106 20-21 0.0547035863811729 1106 22-23 -0.08208494092471597 1106 24-25 -0.10539423616595656 1106 26-27 -3.971937308477891E-4 1106 28-29 0.01297797331300643 1106 30-31 -0.03263932745556275 1106 32-33 0.04306746012230889 1106 34-35 0.038804436082436666 1106 36-37 0.0435164561270156 1106 38-39 0.03765488484441448 1106 40-41 -0.00354764463344992 1106 42-43 -0.03803848714893121 1106 44-45 -0.010980864008089952 1106 46-47 -0.2395099142875452 1106 48-49 -0.0022262836759878724 1106 50-51 0.14878600066549552 1106 52-53 0.2622896274768891 1106 54-55 0.17887815306889365 1106 56-57 0.2863050273453709 1106 58-59 0.3551017818730884 1106 60-61 0.2651494504901777 1106 62-63 0.38310090113484563 1106 64-65 0.26628234052986954 1106 66-67 0.31940964495298374 1106 68-69 0.4808080065576519 1106 70-71 0.34439083126797243 1106 72-73 -0.004438070916890524 1106 74-75 0.3125987340484997 1106 76-77 0.4001512465550192 1106 78-79 0.29081821173065237 1106 80-81 0.2659760451399791 1106 82-83 0.3724962039986508 1106 84-85 0.5626035189063536 1106 86-87 0.5880653252827486 1106 88-89 0.5822997941653973 1106 90-91 0.5178230432636468 1106 92-93 0.5263894824412247 1106 94-95 0.7914941178980435 1106 96-97 0.730792676975252 1106 98-99 0.7251768153463054 1106 100-101 0.5139760934347173 1107 1 -0.47879262122205546 1107 2 -0.497312825006869 1107 3 -0.36734349752101636 1107 4 -0.4540867605592851 1107 5 -0.5109028170285654 1107 6 -0.4280889081969832 1107 7 -0.4168914360442528 1107 8 -0.5086453510084183 1107 9 -0.4851216532947973 1107 10-11 -0.5389352291828118 1107 12-13 -0.5597598128754981 1107 14-15 -0.7466832489897968 1107 16-17 -0.5773451372547456 1107 18-19 -0.5589791267062765 1107 20-21 -0.49926837132415613 1107 22-23 -0.5684014467791201 1107 24-25 -0.6765405227691375 1107 26-27 -0.5501373190172671 1107 28-29 -0.5629834842977246 1107 30-31 -0.5868976991526083 1107 32-33 -0.47997966943141535 1107 34-35 -0.4982415057728957 1107 36-37 -0.5457488000164403 1107 38-39 -0.5985454788225297 1107 40-41 -0.40617345657770443 1107 42-43 -0.37337146312169267 1107 44-45 -0.5280461453769973 1107 46-47 -0.7220084428028102 1107 48-49 -0.7814483937850909 1107 50-51 -0.746562531002958 1107 52-53 -0.7626336451051472 1107 54-55 -0.7490821588858623 1107 56-57 -0.5548981216319433 1107 58-59 -0.6712469915392774 1107 60-61 -0.7610022490746857 1107 62-63 -0.5647520449877916 1107 64-65 -0.5075517705882504 1107 66-67 -0.39548230602555634 1107 68-69 -0.5047294006862586 1107 70-71 -0.671581347160064 1107 72-73 -0.6522526533305495 1107 74-75 -0.5539914679653286 1107 76-77 -0.6860554234750396 1107 78-79 -0.7909293340430281 1107 80-81 -0.5599919815989978 1107 82-83 -0.5143755245754811 1107 84-85 -0.38608370650408474 1107 86-87 -0.558682430703044 1107 88-89 -0.6705827393108805 1107 90-91 -0.6517760822540026 1107 92-93 -0.6670595201023417 1107 94-95 -0.7327998321976033 1107 96-97 -0.6707584255872305 1107 98-99 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2436.0 7 2666.0 8 2713.0 9 2905.0 10 2969.0 11 3219.0 12 3665.0 13 4238.0 14 4357.0 15 5043.0 16 6275.0 17 8475.0 18 7060.0 19 4910.0 20 4771.0 21 4747.0 22 5088.0 23 5653.0 24 6570.0 25 7613.0 26 8453.0 27 10059.0 28 11805.0 29 14815.0 30 18223.0 31 23240.0 32 30863.0 33 42074.0 34 60521.0 35 92613.0 36 151718.0 37 276901.0 38 278396.0 39 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.34092722818182 23.860784803362595 13.425582333454988 23.3727056350006 2 13.840820781488757 21.486313032089065 44.116219166120935 20.556647020301245 3 17.00593553163958 30.504336832252694 30.76383080154998 21.72589683455775 4 10.756791918239406 17.98916436376654 43.554804657315366 27.69923906067869 5 11.458350614300171 38.34925219617441 37.28838273725275 12.90401445227266 6 25.38821468828882 41.27487215218278 20.820618401878274 12.516294757650131 7 23.75939376953079 32.716206222436426 27.282204683984297 16.242195324048485 8 23.318202019196598 36.465681254021845 22.420888023909747 17.795228702871807 9 25.239870183278367 15.506759323046134 24.337317417949947 34.91605307572555 10-11 22.606820703968456 27.341009877700913 31.085595390580867 18.966574027749765 12-13 23.225180333823303 25.469121147919793 30.815593904339146 20.490104613917758 14-15 20.6711366617013 27.007874153253482 28.571615677056684 23.749373507988537 16-17 19.052296741418708 29.147358705892934 30.04563183855445 21.75471271413391 18-19 18.766791965178363 29.69491777012307 31.648352128110087 19.889938136588476 20-21 19.670664721336724 28.28813584479969 30.585118225942914 21.456081207920672 22-23 19.817961472338858 28.972581150730765 29.955374410529227 21.254082966401146 24-25 19.689829888405466 29.112718249342752 30.865916300171047 20.331535562080735 26-27 19.900865008246697 28.355803291169966 30.82108115989861 20.922250540684725 28-29 19.42553393980828 29.71260107551949 29.9616172781621 20.900247706510132 30-31 20.79429358241939 28.711515776992545 29.4549845587253 21.039206081862766 32-33 20.863314377717064 29.187152440350054 29.29127299492625 20.658260187006626 34-35 20.938796322732685 28.35017161337528 30.464670171403824 20.246361892488217 36-37 20.690827874098385 28.754604660584455 30.580970866326666 19.97359659899049 38-39 21.28826594092239 28.141930504222884 30.883116928474198 19.686686626380524 40-41 20.381226294503467 28.20028568834126 30.97601160914482 20.44247640801045 42-43 20.54195949208378 28.423732676042317 29.991827518735153 21.042480313138746 44-45 20.10239247567666 29.7466862365199 29.767990716834642 20.382930570968803 46-47 21.119970397103348 28.51994745661633 29.3364442157232 21.023637930557122 48-49 21.279802505353636 27.95730950390348 29.665210079315436 21.09767791142744 50-51 20.816034867398386 28.403672152522084 28.84963522069893 21.930657759380605 52-53 19.837660660163635 29.33142078010104 29.048697423394977 21.78222113634035 54-55 20.341169141105393 29.659246780004437 28.674783403609403 21.32480067528076 56-57 21.139646104227204 28.479554201095326 29.025642102996457 21.355157591681014 58-59 21.253594422244497 27.996314759780986 29.74763545008513 21.002455367889393 60-61 20.768486874636256 29.798973425459575 28.76417228598809 20.66836741391608 62-63 20.8829790259665 28.856437149001618 28.146991067385123 22.11359275764676 64-65 19.82669038133804 29.364276166536495 28.392543442258933 22.41649000986653 66-67 20.734295991607148 27.636657313079183 28.861417282874708 22.767629412438957 68-69 19.956224187448658 28.864260863251324 28.43389493630024 22.74562001299977 70-71 20.897597424186745 27.91397623672284 29.189764992038075 21.99866134705234 72-73 21.59894070628962 27.768376687798597 29.615148498131898 21.01753410777988 74-75 21.08681710390908 28.968673481115214 28.638318069401397 21.30619134557431 76-77 20.710156329574072 28.54542548969335 29.40724012096137 21.337178059771215 78-79 20.18335205389771 28.528957757210698 29.55944271835732 21.72824747053427 80-81 20.35050412818697 28.386746029175264 30.11794944973557 21.144800392902198 82-83 21.083318598711173 27.468501462666552 30.69748449363377 20.750695444988505 84-85 22.16454691306049 28.125035557769014 28.849801357891025 20.860616171279474 86-87 20.743299826999156 29.11375789857793 29.274682809911713 20.868259464511198 88-89 21.193903156715653 28.355878176897715 29.009098884462325 21.441119781924307 90-91 21.006396312307377 27.3358880074286 30.492954079317425 21.1647616009466 92-93 21.33043234064443 28.316216951560598 29.190888993287302 21.162461714507675 94-95 19.877704860538675 28.333675155667642 29.777156618329055 22.011463365464635 96-97 20.153720933078983 28.378704453299715 29.337853834411874 22.129720779209425 98-99 19.799856259530237 29.2006491053817 29.19531566945987 21.80417896562819 100-101 20.23413067217597 27.91806519357594 29.240585680774778 22.607218453473315 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 72.0 1 46.5 2 39.5 3 49.0 4 34.0 5 39.0 6 59.5 7 98.5 8 150.0 9 192.0 10 186.5 11 190.5 12 240.0 13 307.5 14 387.0 15 459.5 16 605.5 17 777.0 18 929.5 19 1074.0 20 1238.5 21 1421.0 22 1637.0 23 1876.0 24 2165.0 25 2667.0 26 3586.5 27 5045.0 28 8338.0 29 15233.0 30 23553.5 31 30606.5 32 38555.0 33 46537.0 34 55506.0 35 62104.5 36 64016.0 37 69449.5 38 66273.0 39 50971.5 40 38875.5 41 31004.5 42 27074.5 43 25843.0 44 25204.5 45 27052.0 46 29993.0 47 33008.5 48 36850.0 49 35884.0 50 31140.5 51 29021.0 52 27726.0 53 26429.5 54 24876.5 55 22441.0 56 20331.5 57 17077.0 58 13744.5 59 12519.0 60 10872.5 61 8605.0 62 6713.0 63 5459.5 64 4376.5 65 3104.0 66 2285.5 67 1861.0 68 1560.5 69 1278.5 70 1073.5 71 900.0 72 758.5 73 648.5 74 527.0 75 438.0 76 371.0 77 312.5 78 256.0 79 212.0 80 172.0 81 131.5 82 116.0 83 101.5 84 74.5 85 66.5 86 61.0 87 49.5 88 37.5 89 26.5 90 18.0 91 11.5 92 12.5 93 13.5 94 8.5 95 7.0 96 5.0 97 2.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.731283402616067E-5 2 0.0049768315394911585 3 8.731283402616067E-5 4 0.001047754008313928 5 0.0017462566805232134 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.3656417013080334E-5 14-15 0.0035798261950725874 16-17 0.004714893037412677 18-19 0.0036671390290987484 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.61938502078482E-4 42-43 0.0 44-45 6.985026722092853E-4 46-47 0.661874938335311 48-49 0.47393406309400016 50-51 0.35527592165244776 52-53 0.2949427533403708 54-55 0.2849454338443753 56-57 0.2539493777650883 58-59 0.2542549726841799 60-61 0.23329989251790131 62-63 0.22566001954061224 64-65 0.22304063451982745 66-67 0.21217018668357046 68-69 0.19296136319781507 70-71 0.2066694781399223 72-73 0.7267047175997352 74-75 0.4779504534592035 76-77 0.5615088356222393 78-79 0.30686095518494166 80-81 0.26525638977147614 82-83 0.2508497721571596 84-85 0.2444759352732499 86-87 0.22159997275839577 88-89 0.22566001954061224 90-91 0.23535174411751608 92-93 0.1964538765588615 94-95 0.16812086191737238 96-97 0.12931030719274395 98-99 0.1378669649273077 100-101 0.13481101573639206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1145307.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.0743857331197 #Duplication Level Percentage of deduplicated Percentage of total 1 86.18110840103468 37.983794145750046 2 7.800243758028638 6.875819044074268 3 2.1310027104249794 2.817679063727824 4 0.9619055564710506 1.6958158613894492 5 0.5156145470465344 1.1362697218068374 6 0.354353596784683 0.937075026636389 7 0.22684887078972138 0.6998757244016165 8 0.19261674864398698 0.6791571902715554 9 0.12986704802758375 0.5151429331910377 >10 1.120035978311239 10.240923712632261 >50 0.18325545907569915 5.6441309182688855 >100 0.1713122891094701 14.574977073759356 >500 0.02029483561051819 6.143077207768997 >1k 0.010943293711553925 8.39287586539435 >5k 5.969069297211232E-4 1.6633865109272148 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 6433 0.5616834612902916 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 6368 0.5560081270785912 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 6167 0.5384582474393328 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 4671 0.40783824773619654 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 4391 0.38339065420887153 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 3942 0.3441871917311254 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 3467 0.30271359556869903 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 2918 0.2547788496883368 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 2885 0.2518975261654735 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 2683 0.2342603336921891 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 2528 0.2207268444181342 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 2501 0.21836939789942783 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 2426 0.21182093534746577 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 2284 0.19942251291575097 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 2282 0.19924788724769868 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2159 0.1885084086624809 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 2125 0.18553977230559143 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 2100 0.1833569514549374 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 1989 0.17366522687803357 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1849 0.1614414301143711 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 1795 0.1567265370769584 No Hit ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA 1775 0.1549802803964352 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 1747 0.1525355210437027 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1661 0.14502661731745287 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 1647 0.14380423764108663 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 1556 0.135858769744706 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 1530 0.13358863606002583 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1450 0.12660360933793297 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 1394 0.12171409063246798 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 1378 0.12031708528804941 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 1363 0.119007392777657 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 1350 0.11787232593531692 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 1341 0.11708651042908146 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 1323 0.11551487941661057 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 1257 0.10975223237088395 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1251 0.109228355366727 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 1231 0.10748209868620379 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 1224 0.10687090884802065 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 1201 0.10486271366541897 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 1198 0.10460077516334049 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 1181 0.10311645698489576 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 1175 0.10259257998073879 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 1169 0.10206870297658183 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 4.3656417013080334E-5 0.0 0.0 0.0 14-15 4.3656417013080334E-5 8.731283402616067E-5 0.0 0.0 0.0 16-17 8.731283402616067E-5 8.731283402616067E-5 0.0 0.0 0.0 18-19 8.731283402616067E-5 8.731283402616067E-5 0.0 0.0 0.0 20-21 8.731283402616067E-5 8.731283402616067E-5 0.0 0.0 0.0 22-23 8.731283402616067E-5 8.731283402616067E-5 0.0 8.731283402616067E-5 0.0 24-25 8.731283402616067E-5 8.731283402616067E-5 0.0 8.731283402616067E-5 0.0 26-27 8.731283402616067E-5 8.731283402616067E-5 0.0 1.7462566805232134E-4 4.3656417013080334E-5 28-29 8.731283402616067E-5 8.731283402616067E-5 0.0 6.985026722092853E-4 8.731283402616067E-5 30-31 8.731283402616067E-5 8.731283402616067E-5 0.0 0.0027940106888371414 8.731283402616067E-5 32-33 8.731283402616067E-5 8.731283402616067E-5 0.0 0.010957760670283164 8.731283402616067E-5 34-35 8.731283402616067E-5 8.731283402616067E-5 0.0 0.024098342191220347 8.731283402616067E-5 36-37 8.731283402616067E-5 8.731283402616067E-5 0.0 0.04671236620399596 8.731283402616067E-5 38-39 8.731283402616067E-5 8.731283402616067E-5 0.0 0.07631141693886442 1.7462566805232134E-4 40-41 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.10979588878789703 1.7462566805232134E-4 42-43 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.14725309458511998 1.7462566805232134E-4 44-45 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.19208823485755347 1.7462566805232134E-4 46-47 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.25320721867586593 1.7462566805232134E-4 48-49 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.3190410955315911 1.7462566805232134E-4 50-51 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.38308505928978 1.7462566805232134E-4 52-53 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.44730364871602113 1.7462566805232134E-4 54-55 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.5224363423955324 1.7462566805232134E-4 56-57 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.6159047312205373 1.7462566805232134E-4 58-59 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.7263991226806437 1.7462566805232134E-4 60-61 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.8427434740205029 1.7462566805232134E-4 62-63 1.7462566805232134E-4 8.731283402616067E-5 0.0 0.9612269897940029 1.7462566805232134E-4 64-65 1.7462566805232134E-4 8.731283402616067E-5 0.0 1.090711922654799 1.7462566805232134E-4 66-67 1.7462566805232134E-4 8.731283402616067E-5 0.0 1.248704495825137 1.7462566805232134E-4 68-69 1.7462566805232134E-4 8.731283402616067E-5 0.0 1.421627563613948 1.7462566805232134E-4 70-71 1.7462566805232134E-4 8.731283402616067E-5 0.0 1.6043733252307022 1.7462566805232134E-4 72-73 1.7462566805232134E-4 8.731283402616067E-5 0.0 1.8038394945634664 1.7462566805232134E-4 74-75 1.7462566805232134E-4 8.731283402616067E-5 0.0 1.9952292267488105 1.7462566805232134E-4 76-77 1.7462566805232134E-4 8.731283402616067E-5 0.0 2.219273958859939 1.7462566805232134E-4 78-79 1.7462566805232134E-4 8.731283402616067E-5 0.0 2.4689013513407323 1.7462566805232134E-4 80-81 1.7462566805232134E-4 8.731283402616067E-5 0.0 2.7569900472100493 2.1828208506540167E-4 82-83 1.7462566805232134E-4 8.731283402616067E-5 0.0 3.063327125390834 2.61938502078482E-4 84-85 1.7462566805232134E-4 8.731283402616067E-5 0.0 3.3705809883288933 2.61938502078482E-4 86-87 1.7462566805232134E-4 8.731283402616067E-5 0.0 3.702369757628304 2.61938502078482E-4 88-89 1.7462566805232134E-4 8.731283402616067E-5 0.0 4.068166875780904 2.61938502078482E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 1165 0.0 57.353203 1 ACCTGGG 1200 0.0 55.67797 3 TACCTGG 1215 0.0 55.383007 2 CTGGGGG 1035 0.0 45.783096 5 CCTGGGG 1195 0.0 44.41152 4 GTACACG 120 3.8835424E-9 35.54068 1 TATCACG 110 7.16027E-8 34.463688 2 GTACTAG 625 0.0 32.60265 1 GCATCGT 630 0.0 32.341076 8 GTACCCG 135 1.2250894E-8 31.591717 1 TATTCTC 1120 0.0 31.30828 5 CGTTAAC 185 9.094947E-12 30.737886 1 TAGGCAT 750 0.0 29.063108 5 TGGGGGG 2580 0.0 29.017763 6 TAGTACT 625 0.0 28.810387 4 CTGTGCG 610 0.0 28.743282 9 CTAGGCA 825 0.0 28.718489 4 AGGGCTA 2405 0.0 27.584078 5 TCACGCA 140 5.929778E-7 27.077433 4 GCTATTG 2500 0.0 26.724258 8 >>END_MODULE