FastQCFastQC Report
Sat 18 Jun 2016
SRR1931080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1145307
Sequences flagged as poor quality0
Sequence length101
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA74280.6485597311463215No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA71920.6279539023161476No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC68850.6011488622701162No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC61020.5327829132276325No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC41920.36601540023766554No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC38050.33222533346954136No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC37810.3301298254529135No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA31880.2783533148754002No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC29570.2581840502153571No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC29000.25320721867586593No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28680.2504132079870288No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA23800.20780454498226242No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23260.2030896519448497No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG22760.1987240102435417No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC22170.1935725530359982No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA21070.18396814129312053No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC20030.17488760655439983No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG18820.1643227536372344No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17700.1545437162263044No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC17660.15419446489019975No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC17570.1534086493839643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16970.14816987934239467No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG16960.1480825665083685No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA16940.1479079408403162No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT16840.14703481250005457No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT16520.14424080181121743No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA16140.1409229141182233No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA16140.1409229141182233No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT15300.13358863606002583No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA14900.13009612269897938No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG14410.12581779383169753No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA13950.12180140346649414No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT13590.11865814144155234No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA13260.11577681791868906No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA13130.11464175107634897No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA13010.11359399706803504No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT12820.11193505322153798No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA12290.10730747301815147No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC12180.1063470318438637No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT12070.10538659066957592No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT11610.10137020030437255No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG11530.10067169763216327No Hit
TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC11470.1001478206280063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10000.052.248492
ACCTGGG10150.051.944313
GTACCTG10350.050.967251
CCTGGGG9600.047.4986274
CTGGGGG9450.042.2215
TAACGCA1650.037.423164
ATAACGC1553.6379788E-1133.7087023
GTACACG1401.6976628E-830.5508371
CGTTAAC2051.8189894E-1230.1368681
TGGGGGG21800.029.4142866
TATTCTC15150.028.5305255
TCTCTGC15200.028.4366788
GCTATTG27100.027.3423828
CTATTGA27700.027.2645539
AGGGCTA27200.027.241865
GCGCGAG350.00839267827.14325540-41
GGGGGGA22300.026.411797
ATTTAGG30000.025.3458791
AGCACCG2251.0186341E-1025.33265
CTCTGCA17400.025.1142149
Produced by FastQC (version 0.11.5)