##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931080_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1145307 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45815838024215 33.0 33.0 33.0 33.0 33.0 2 32.50099667600041 33.0 33.0 33.0 33.0 33.0 3 32.52327803811554 33.0 33.0 33.0 33.0 33.0 4 36.451691991754174 37.0 37.0 37.0 37.0 37.0 5 36.314381209579615 37.0 37.0 37.0 33.0 37.0 6 36.3175253447329 37.0 37.0 37.0 33.0 37.0 7 36.24554027872003 37.0 37.0 37.0 33.0 37.0 8 36.26167394419138 37.0 37.0 37.0 33.0 37.0 9 36.281036438265026 37.0 37.0 37.0 33.0 37.0 10-11 36.19879298738242 37.0 37.0 37.0 33.0 37.0 12-13 36.12870653894545 37.0 37.0 37.0 33.0 37.0 14-15 37.776969843020254 40.0 37.0 40.0 33.0 40.0 16-17 37.76930639557778 40.0 37.0 40.0 33.0 40.0 18-19 37.681856043838025 40.0 37.0 40.0 33.0 40.0 20-21 37.592118532410964 40.0 37.0 40.0 33.0 40.0 22-23 37.44623013742167 40.0 37.0 40.0 33.0 40.0 24-25 37.59709405425794 40.0 37.0 40.0 33.0 40.0 26-27 37.50496286148605 40.0 37.0 40.0 33.0 40.0 28-29 37.39570001754988 40.0 37.0 40.0 33.0 40.0 30-31 37.231701631091056 38.5 37.0 40.0 33.0 40.0 32-33 37.059360503341026 37.0 37.0 40.0 33.0 40.0 34-35 36.94816804577288 37.0 37.0 40.0 33.0 40.0 36-37 36.868386380245646 37.0 37.0 40.0 33.0 40.0 38-39 36.68447804824383 37.0 37.0 40.0 33.0 40.0 40-41 36.46042502141347 37.0 37.0 40.0 33.0 40.0 42-43 36.236123589570305 37.0 37.0 40.0 33.0 40.0 44-45 35.96951385087142 37.0 37.0 40.0 33.0 40.0 46-47 35.682441039826 37.0 37.0 40.0 30.0 40.0 48-49 35.38580703689055 37.0 37.0 40.0 27.0 40.0 50-51 35.11183420689824 37.0 35.0 40.0 27.0 40.0 52-53 34.87080538231234 37.0 33.0 40.0 27.0 40.0 54-55 34.627089941823456 37.0 33.0 40.0 27.0 40.0 56-57 34.293123153879264 37.0 33.0 40.0 27.0 40.0 58-59 33.98373711153429 37.0 33.0 40.0 27.0 40.0 60-61 33.69992237889055 37.0 33.0 38.5 24.5 40.0 62-63 33.36328338166099 37.0 33.0 37.0 22.0 40.0 64-65 32.958745995615146 37.0 33.0 37.0 22.0 40.0 66-67 32.56976033500188 37.0 33.0 37.0 22.0 40.0 68-69 32.27245184042357 37.0 33.0 37.0 22.0 40.0 70-71 31.933795916728005 37.0 33.0 37.0 18.5 40.0 72-73 31.517731490334032 35.0 33.0 37.0 15.0 40.0 74-75 31.08938695039845 33.0 30.0 37.0 15.0 37.0 76-77 29.74704555197864 33.0 27.0 37.0 15.0 37.0 78-79 30.466852119126138 33.0 27.0 37.0 15.0 37.0 80-81 30.47820846288375 33.0 27.0 37.0 15.0 37.0 82-83 30.16514131145623 33.0 27.0 37.0 10.5 37.0 84-85 29.743826764352264 33.0 27.0 37.0 6.0 37.0 86-87 29.271500567096858 33.0 27.0 37.0 6.0 37.0 88-89 28.80366530545958 33.0 27.0 37.0 2.0 37.0 90-91 28.246922440882663 33.0 27.0 37.0 2.0 37.0 92-93 27.734841837166805 33.0 27.0 33.0 2.0 37.0 94-95 27.11358395609212 33.0 27.0 33.0 2.0 37.0 96-97 26.4458979120882 33.0 24.5 33.0 2.0 37.0 98-99 25.609550103160114 33.0 22.0 33.0 2.0 37.0 100-101 23.947684769236545 30.0 8.5 33.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.166218295195101 1101 2 0.30374196853448865 1101 3 0.23853399720221802 1101 4 0.29888264796240094 1101 5 0.20911426483065298 1101 6 0.22483518387932833 1101 7 0.18503608461999477 1101 8 0.17460040996444803 1101 9 0.21768310805192925 1101 10-11 0.26060273832830916 1101 12-13 0.291328883935293 1101 14-15 0.3087180430649852 1101 16-17 0.31023440098469024 1101 18-19 0.2676708749016967 1101 20-21 0.18494244291927942 1101 22-23 0.23080403563808716 1101 24-25 0.28538847413211954 1101 26-27 0.34545919440368067 1101 28-29 0.30925265314892414 1101 30-31 0.19992902608436225 1101 32-33 0.16061682743173833 1101 34-35 0.24191146308192657 1101 36-37 0.17183608498099545 1101 38-39 0.11698525016991113 1101 40-41 0.23602947097943883 1101 42-43 0.24382234637223377 1101 44-45 -0.04485867242739516 1101 46-47 -0.10742516604643271 1101 48-49 -0.2316858630429266 1101 50-51 -0.0886913926244901 1101 52-53 -0.11689947410608426 1101 54-55 -0.12738400805812944 1101 56-57 -0.23405239010754997 1101 58-59 -0.24320216799821992 1101 60-61 -0.18855450261554552 1101 62-63 -0.3357122247099724 1101 64-65 -0.3995205452985431 1101 66-67 -0.4520209700636002 1101 68-69 -0.5844410594467107 1101 70-71 -0.3366866288607646 1101 72-73 -0.6012182033958773 1101 74-75 -0.7904563909254811 1101 76-77 -0.7294824527660673 1101 78-79 -0.7806294833352219 1101 80-81 -1.1240773612606034 1101 82-83 -1.0420696357751957 1101 84-85 -1.1151043209246403 1101 86-87 -1.1795226039727176 1101 88-89 -1.5505028714627933 1101 90-91 -1.587550154472428 1101 92-93 -1.6013270491151168 1101 94-95 -1.627884544936098 1101 96-97 -1.889374007636384 1101 98-99 -2.357146800639562 1101 100-101 -2.9819877228736225 1102 1 0.12328568465552792 1102 2 0.13605178051791 1102 3 0.1839869290416587 1102 4 0.23712219124055167 1102 5 0.1400458511214353 1102 6 0.2170703886490699 1102 7 0.3000080123464386 1102 8 0.3368667350208554 1102 9 0.2062134128677897 1102 10-11 0.2888034067088441 1102 12-13 0.16023231211348588 1102 14-15 0.3417518854674313 1102 16-17 0.25713968561382927 1102 18-19 0.25241189562964195 1102 20-21 0.2451781656330141 1102 22-23 0.30051987934120916 1102 24-25 0.41417866753182153 1102 26-27 0.2838027629505149 1102 28-29 0.3723621648412134 1102 30-31 0.3125716607316136 1102 32-33 0.4723052666598875 1102 34-35 0.506756159902821 1102 36-37 0.46134495477191706 1102 38-39 0.4374023551248527 1102 40-41 0.5486261308226332 1102 42-43 0.34913010840315195 1102 44-45 0.2659524627865366 1102 46-47 0.31226223438392253 1102 48-49 0.304180667445209 1102 50-51 0.2549200737244277 1102 52-53 0.13927220686951358 1102 54-55 0.05163984209892902 1102 56-57 0.13973949824933385 1102 58-59 0.23948126201314324 1102 60-61 0.11227602640023093 1102 62-63 -0.018931639462344663 1102 64-65 0.0038752613919541545 1102 66-67 -0.17530466224773278 1102 68-69 -0.2059680339990777 1102 70-71 -0.2606092398804627 1102 72-73 -0.39049501681819265 1102 74-75 -0.22441052960353147 1102 76-77 -0.27244307010875346 1102 78-79 -0.3460410087530299 1102 80-81 -0.48267574079424946 1102 82-83 -0.2579671701580928 1102 84-85 -0.41595667342335574 1102 86-87 -0.7639132850545245 1102 88-89 -0.6810469454414871 1102 90-91 -0.848339320713535 1102 92-93 -0.7913226553205526 1102 94-95 -1.1251751595290251 1102 96-97 -0.9261054754983924 1102 98-99 -1.0120404257092162 1102 100-101 -1.6271804460514723 1103 1 0.2670253203378934 1103 2 0.263824565138421 1103 3 0.15584661991599802 1103 4 0.24362454787012666 1103 5 0.1980649320148018 1103 6 0.22198618713930784 1103 7 0.23818709931598647 1103 8 0.13614181297740657 1103 9 0.14942011691984192 1103 10-11 0.2824265822724712 1103 12-13 0.2503897442522529 1103 14-15 0.2862234054829571 1103 16-17 0.2257387041419534 1103 18-19 0.18456965689483695 1103 20-21 0.2695968144424583 1103 22-23 0.16262645722813573 1103 24-25 0.2256999911263904 1103 26-27 0.2095657666088826 1103 28-29 0.3539574574894644 1103 30-31 0.27175576476931695 1103 32-33 0.39910522952767025 1103 34-35 0.3459652437920866 1103 36-37 0.45015254662139625 1103 38-39 0.4352387708537222 1103 40-41 0.15211951242783783 1103 42-43 0.304501831379973 1103 44-45 0.5053950998487124 1103 46-47 0.33521036321582187 1103 48-49 0.471152198009662 1103 50-51 0.34544038884188666 1103 52-53 0.2988067461558188 1103 54-55 0.21943524361206102 1103 56-57 0.30050509694295613 1103 58-59 0.17374986293047812 1103 60-61 0.03436483989948158 1103 62-63 -0.25519483089156125 1103 64-65 -0.11144451868408112 1103 66-67 -0.05259641594302167 1103 68-69 -0.12319484851946072 1103 70-71 0.08366332743221605 1103 72-73 -0.052283861120297814 1103 74-75 -0.27706231806807224 1103 76-77 -0.5678329794302037 1103 78-79 -0.28061447356814995 1103 80-81 -0.32224559787743345 1103 82-83 -0.07578514636815825 1103 84-85 -0.019873066872893475 1103 86-87 -0.1102217946603723 1103 88-89 -0.48617351142922516 1103 90-91 -0.5894581817673306 1103 92-93 -0.4802697039426498 1103 94-95 -0.6220832699635999 1103 96-97 -0.6020731282784553 1103 98-99 -0.8248096869891803 1103 100-101 -1.5074596549665777 1104 1 0.225072909697829 1104 2 0.21796965114136668 1104 3 0.17623035608838933 1104 4 0.18939913384109985 1104 5 0.22183763167590342 1104 6 0.17152340179497827 1104 7 0.20967464647373646 1104 8 0.16683631070743843 1104 9 0.08807108565186184 1104 10-11 0.24596125520552192 1104 12-13 0.14261630925001612 1104 14-15 0.26664694599065797 1104 16-17 0.3129365748498074 1104 18-19 0.12529276898639807 1104 20-21 0.07058612801750286 1104 22-23 0.08749477521521953 1104 24-25 0.1836067067092344 1104 26-27 0.2090252509109476 1104 28-29 0.21192272061370687 1104 30-31 0.13351562793192784 1104 32-33 0.23972603328750353 1104 34-35 0.25498276409389575 1104 36-37 0.3156011047133447 1104 38-39 0.31826757704337894 1104 40-41 0.1879983649759609 1104 42-43 0.29479952464681247 1104 44-45 0.3308664774943395 1104 46-47 0.3622101713877015 1104 48-49 0.22411754106228443 1104 50-51 0.56670014010475 1104 52-53 0.5156734336038653 1104 54-55 0.5336537038716713 1104 56-57 0.6621550585773264 1104 58-59 0.5653000339772234 1104 60-61 0.6735171594211877 1104 62-63 0.3267137789939838 1104 64-65 0.4646063397467657 1104 66-67 0.5000154438306197 1104 68-69 0.4856156572673527 1104 70-71 0.6432389476764762 1104 72-73 0.48835073028580567 1104 74-75 0.25315560667135983 1104 76-77 0.27371199627156884 1104 78-79 0.6826424482299167 1104 80-81 0.3274712558216528 1104 82-83 0.2343209985260124 1104 84-85 0.30682820878411476 1104 86-87 0.4458050053268394 1104 88-89 0.41625391359795216 1104 90-91 0.07004989899230907 1104 92-93 -0.031040213566026154 1104 94-95 0.06614008191311882 1104 96-97 -0.08317506094678606 1104 98-99 0.0496867641905645 1104 100-101 -0.524498388261545 1105 1 0.16828417538249596 1105 2 0.15625388463836742 1105 3 0.14446889945990904 1105 4 0.233026384250266 1105 5 0.13388414013198968 1105 6 0.21615651690681403 1105 7 0.07606512656102637 1105 8 0.1423034410947821 1105 9 0.07392078559730209 1105 10-11 0.14979036049739847 1105 12-13 0.15859080461280683 1105 14-15 0.10371391271188202 1105 16-17 0.1368250091105736 1105 18-19 0.14777981208514746 1105 20-21 0.0880012262171661 1105 22-23 0.14069478885406284 1105 24-25 -0.051416138353509666 1105 26-27 0.11030559733757173 1105 28-29 0.1659427697135456 1105 30-31 0.274438977631867 1105 32-33 0.21488628969776613 1105 34-35 0.04078472808735256 1105 36-37 0.1628460583412732 1105 38-39 0.33678230699427303 1105 40-41 0.17551029896396386 1105 42-43 0.3542734062616475 1105 44-45 0.02824611470110483 1105 46-47 -0.10655687716658235 1105 48-49 -0.08609795266384879 1105 50-51 -0.012251714777953282 1105 52-53 0.045039568355640824 1105 54-55 -0.11565753453530903 1105 56-57 -0.01073433040248517 1105 58-59 0.04249759262424391 1105 60-61 -0.22067098842387622 1105 62-63 -0.3060092160960366 1105 64-65 0.0316327309087967 1105 66-67 0.04682224961349135 1105 68-69 -0.01632787792086532 1105 70-71 -0.14961259351282408 1105 72-73 -0.12990656649542842 1105 74-75 0.05338917186503167 1105 76-77 -0.28579598245183746 1105 78-79 0.0440036048038408 1105 80-81 0.17521914180092324 1105 82-83 0.14103130949164466 1105 84-85 0.13363194582721505 1105 86-87 -0.04726475735728641 1105 88-89 -0.025883293166916843 1105 90-91 -0.054447168656356126 1105 92-93 -0.10128025721032685 1105 94-95 -0.10686864694667264 1105 96-97 -0.22594817878639262 1105 98-99 -0.3859378948383494 1105 100-101 -0.9666526435806979 1106 1 0.06531630903776176 1106 2 0.1599917379654343 1106 3 0.16070452465908858 1106 4 0.14532862102058886 1106 5 0.12754815465127933 1106 6 0.2716598577482685 1106 7 0.21482347866464835 1106 8 0.1323970494736244 1106 9 0.019129095298978882 1106 10-11 0.19884428389300268 1106 12-13 0.15352485202736688 1106 14-15 0.2660202006238137 1106 16-17 0.20070948675637368 1106 18-19 0.31716411958299773 1106 20-21 0.23760583479586472 1106 22-23 0.3158006586881825 1106 24-25 0.22990256225632066 1106 26-27 0.16250725776547625 1106 28-29 0.22668693742000556 1106 30-31 0.2423789181071001 1106 32-33 0.14408786370131565 1106 34-35 0.2896044954978052 1106 36-37 -0.04480128167914188 1106 38-39 0.20207374612182605 1106 40-41 0.19407938173147699 1106 42-43 0.27329581177551887 1106 44-45 0.2161522416476771 1106 46-47 0.11643078575683319 1106 48-49 0.2454427505063066 1106 50-51 -0.004455292746229134 1106 52-53 0.37308886264676744 1106 54-55 0.37531719714407785 1106 56-57 0.4377722837613902 1106 58-59 0.46016151531766525 1106 60-61 0.39577772682407897 1106 62-63 0.6043302425634138 1106 64-65 0.7013296515010126 1106 66-67 0.5754827261357747 1106 68-69 0.6349397242894455 1106 70-71 0.650882861106286 1106 72-73 0.7315795002781904 1106 74-75 0.6318010433304089 1106 76-77 0.5907063158958366 1106 78-79 0.6232047736932351 1106 80-81 0.6346597871747903 1106 82-83 0.8305700465077344 1106 84-85 0.9051637634934373 1106 86-87 0.9597322393963985 1106 88-89 0.9834188031638327 1106 90-91 0.9565098190227737 1106 92-93 1.3913702967157668 1106 94-95 1.2849138823700876 1106 96-97 1.0664227113304392 1106 98-99 0.9505816613725884 1106 100-101 0.9283053055693777 1107 1 -0.03959004146989997 1107 2 0.09558274929586474 1107 3 0.020500934236352464 1107 4 0.004670446922922622 1107 5 -0.0633218081411826 1107 6 -0.07756813829974618 1107 7 -0.09813504648722926 1107 8 0.07337211831809043 1107 9 -0.17465756043665692 1107 10-11 -0.010831779254708351 1107 12-13 0.03181992675776968 1107 14-15 0.06539245472821165 1107 16-17 -0.042199796210134366 1107 18-19 -0.006439020985837374 1107 20-21 -0.06820153734262391 1107 22-23 -0.05992325924501074 1107 24-25 0.044875182129779034 1107 26-27 0.06945161462154914 1107 28-29 -0.03836819070428277 1107 30-31 0.024051313053597312 1107 32-33 0.0013880045434220278 1107 34-35 -0.16322522218734292 1107 36-37 -0.06416575793568313 1107 38-39 0.051119120404472085 1107 40-41 -0.059855526090508704 1107 42-43 0.0959871573431954 1107 44-45 0.05601397463626512 1107 46-47 0.006430733203799832 1107 48-49 -0.08021652720489669 1107 50-51 -0.10160481661581144 1107 52-53 -0.007847001640293172 1107 54-55 0.126612629235062 1107 56-57 -0.014560807828026157 1107 58-59 0.18524481186387476 1107 60-61 0.2516523668358559 1107 62-63 0.18791845807286478 1107 64-65 0.29764620990845003 1107 66-67 0.3362298199534379 1107 68-69 0.15768204629234361 1107 70-71 0.08949435760625235 1107 72-73 0.2512146246186475 1107 74-75 0.3813990126814879 1107 76-77 0.3378579418864618 1107 78-79 0.33662129846672784 1107 80-81 0.2846434957367272 1107 82-83 0.2419459372815247 1107 84-85 0.24053615423569497 1107 86-87 0.4776291724018016 1107 88-89 0.3921644149861585 1107 90-91 0.4622315297288786 1107 92-93 0.9264152821060279 1107 94-95 0.6634677807535567 1107 96-97 0.7344005759958883 1107 98-99 1.0815543127778575 1107 100-101 1.7157178047810788 1108 1 0.1279731694735986 1108 2 -0.0029265226875381245 1108 3 0.03946748298938019 1108 4 0.11490751082097006 1108 5 0.05619442370804251 1108 6 0.06240837476567407 1108 7 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0.241755466528506 2316 96-97 0.392699939053216 2316 98-99 0.4772252257290255 2316 100-101 0.6911105860974267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 11.0 5 21.0 6 63.0 7 135.0 8 354.0 9 701.0 10 1225.0 11 1812.0 12 2536.0 13 3464.0 14 4374.0 15 5771.0 16 7570.0 17 9355.0 18 8907.0 19 7226.0 20 6699.0 21 7109.0 22 7631.0 23 8903.0 24 10264.0 25 12069.0 26 14499.0 27 18088.0 28 22335.0 29 27526.0 30 34623.0 31 43872.0 32 56501.0 33 75376.0 34 103529.0 35 145795.0 36 209277.0 37 237558.0 38 50125.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.47907086871469 29.718326717941412 18.35249219564319 25.45011021770071 2 9.990797203187975 25.659737502008202 41.121396589901025 23.228068704902803 3 12.450548193628434 32.199401557835586 33.20480884164682 22.145241406889156 4 9.107339778766741 21.746221755389602 40.41466611135704 28.731772354486612 5 10.747773304450249 39.44523171516458 37.410493431018935 12.39650154936624 6 26.02105810931043 43.088097776404055 19.412698953206434 11.478145161079082 7 23.101491565143668 33.493814322273415 27.039125754055465 16.36556835852745 8 23.831601483270422 37.72935990088247 21.11250520602773 17.326533409819376 9 24.69835598664812 15.00043219852843 23.83404624262316 36.467165572200294 10-11 22.400631446415677 28.768967621781762 30.67775714284467 18.152643788957896 12-13 22.793320917448334 25.910738343518375 31.109126199350918 20.186814539682373 14-15 20.393668405942876 27.44847656168144 28.346797007606718 23.81105802476897 16-17 19.215677543226402 29.413336336894822 29.70849737232026 21.662488747558513 18-19 19.0754967881974 29.580846008974014 31.797587895647194 19.546069307181394 20-21 19.485517856784252 28.689687568486004 31.0016004442477 20.823194130482044 22-23 19.825033811894976 28.980526618627145 30.3160637278913 20.87837584158658 24-25 19.376638752753628 29.521473281836226 31.20909939431087 19.892788571099278 26-27 19.820493544525615 28.71046802298423 31.01910666746994 20.449931765020207 28-29 19.363978391819835 30.171342705492936 30.00051514572075 20.46416375696647 30-31 20.776001543690906 28.922900148169877 29.456075969150632 20.845022338988585 32-33 21.08133452428039 29.531121349996113 29.554084625344995 19.8334595003785 34-35 20.86191737237265 28.887713076057338 30.557789308892723 19.69258024267729 36-37 20.726757105300152 29.000914165372254 30.863384227984287 19.40894450134331 38-39 20.88406941027114 28.19111224423866 31.552702945762967 19.372115399727235 40-41 20.207854916307944 28.64852829463795 31.16775956761288 19.97585722144123 42-43 20.48915043740103 28.687500027287083 30.434833860956374 20.38851567435551 44-45 20.160358750972446 29.79253597507044 30.051287558706967 19.995817715250148 46-47 20.92321098185901 28.76599898542487 29.679727793508643 20.63106223920748 48-49 21.289244970339805 28.128683513401658 29.920693683609446 20.66137783264909 50-51 20.627700695097474 28.75665651218407 28.64214573035876 21.973497062359698 52-53 19.691750770754044 29.42538550799043 29.14498034151542 21.737883379740104 54-55 20.273123276117232 29.9032049921986 28.56862832410873 21.255043407575435 56-57 21.38566340727857 28.820875101610312 29.25709002040501 20.536371470706108 58-59 21.428577665202432 28.485943070285956 29.76861225854727 20.31686700596434 60-61 20.894048495294275 30.518454877163943 28.52444803009149 20.06304859745029 62-63 21.200123634972982 28.877322848808223 28.1279604507787 21.794593065440097 64-65 20.04872929267 29.50427265353307 28.244086520033495 22.202911533763437 66-67 21.099676190883663 27.863082486410452 28.472980471476898 22.564260851228987 68-69 20.394189367723502 29.20518576925999 28.237853111784638 22.16277175123187 70-71 21.183864544528618 28.187627342034755 29.061247726015605 21.56726038742102 72-73 21.471448266709274 28.359077522445947 29.39906068853155 20.77041352231323 74-75 21.035198874711703 29.51476307870426 28.593874216203265 20.856163830380776 76-77 20.75024415416136 28.89121922533388 29.25038887853238 21.10814774197238 78-79 19.99975551008237 29.043088291858094 29.769878230923347 21.18727796713619 80-81 20.311824661495784 28.865915919455777 30.200723155198954 20.62153626384949 82-83 20.8323200959785 28.205122607904865 30.586728824503506 20.37582847161313 84-85 21.899332172919934 28.923325837359638 28.43209289593868 20.745249093781748 86-87 20.844352979080643 29.381220515444383 28.712183588098522 21.06224291737645 88-89 20.85571816115679 28.793808123062202 28.680388751662218 21.67008496411879 90-91 20.859385300185888 27.610544596339672 30.303534336208543 21.226535767265894 92-93 21.182648814474195 28.60423987388386 28.70683366316145 21.5062776484805 94-95 19.38664582714842 28.739063618741596 29.337181076786056 22.537109477323927 96-97 19.924620164826266 28.904320522777887 28.713098654330672 22.457960658065176 98-99 19.603171900634504 29.31205344942448 28.903734981101138 22.181039668839883 100-101 18.491831419283805 29.08090697396235 29.039520546091296 23.387741060662552 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 64.0 1 47.0 2 37.0 3 44.0 4 35.5 5 30.5 6 52.5 7 116.0 8 179.0 9 219.0 10 230.5 11 236.0 12 273.0 13 365.5 14 493.0 15 602.5 16 699.0 17 799.5 18 895.5 19 1009.0 20 1157.0 21 1335.5 22 1539.0 23 1812.0 24 2138.5 25 2602.5 26 3571.5 27 5199.0 28 8824.5 29 15502.5 30 23428.0 31 30756.5 32 39752.0 33 48463.5 34 56908.0 35 64086.5 36 66363.5 37 71515.5 38 67590.0 39 49723.5 40 37658.0 41 31510.0 42 28241.0 43 26837.0 44 25684.0 45 27721.0 46 30620.5 47 34407.5 48 38322.5 49 36282.5 50 31304.5 51 28839.5 52 27157.0 53 25902.0 54 24783.5 55 22406.0 56 19161.5 57 15718.0 58 13144.0 59 11917.0 60 10016.0 61 7429.0 62 5433.5 63 4050.0 64 2998.5 65 2124.0 66 1531.0 67 1113.0 68 856.0 69 687.5 70 545.0 71 455.5 72 354.5 73 270.5 74 217.0 75 180.5 76 158.0 77 117.0 78 96.5 79 79.0 80 61.0 81 48.0 82 39.5 83 46.0 84 34.0 85 22.5 86 20.5 87 12.0 88 9.5 89 7.0 90 3.5 91 1.5 92 2.5 93 4.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06583387685572514 2 2.61938502078482E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.61938502078482E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.731283402616067E-5 40-41 0.0032305748589679453 42-43 0.006679431803001291 44-45 0.0 46-47 0.0 48-49 8.731283402616067E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0029249799398763827 68-69 0.008382032066511424 70-71 0.0013533489274054904 72-73 0.0 74-75 4.3656417013080334E-5 76-77 0.002313790101693258 78-79 0.005718990628713524 80-81 0.004191016033255712 82-83 0.004409298118321114 84-85 0.002226477267667097 86-87 3.492513361046427E-4 88-89 0.0 90-91 0.0 92-93 0.0011787232593531692 94-95 0.0041037031992295515 96-97 0.0035361697780595075 98-99 0.0 100-101 3.492513361046427E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1145307.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.403477236117766 #Duplication Level Percentage of deduplicated Percentage of total 1 84.77310931878449 35.09901501915217 2 8.568488828785073 7.095304643410643 3 2.367082434353936 2.940163310603622 4 1.05093142613642 1.740488615150402 5 0.5918525946088966 1.2252377714013343 6 0.3788697217278878 0.9411914339408924 7 0.27398217043789247 0.7940670189779194 8 0.19643536875739367 0.6506485854972119 9 0.16047730635901244 0.5979886650673982 >10 1.2081054930666728 10.261923531851373 >50 0.19652697841707353 5.7388589588759835 >100 0.20075039770112896 16.481091642652224 >500 0.02197688563208562 6.395334096312196 >1k 0.010565810400041164 7.623287938406724 >5k 8.452648320032931E-4 2.4153987686999394 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 7428 0.6485597311463215 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 7192 0.6279539023161476 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 6885 0.6011488622701162 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 6102 0.5327829132276325 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 4192 0.36601540023766554 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 3805 0.33222533346954136 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 3781 0.3301298254529135 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 3188 0.2783533148754002 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 2957 0.2581840502153571 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 2900 0.25320721867586593 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2868 0.2504132079870288 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 2380 0.20780454498226242 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2326 0.2030896519448497 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 2276 0.1987240102435417 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 2217 0.1935725530359982 No Hit ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA 2107 0.18396814129312053 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 2003 0.17488760655439983 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 1882 0.1643227536372344 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1770 0.1545437162263044 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 1766 0.15419446489019975 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 1757 0.1534086493839643 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1697 0.14816987934239467 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 1696 0.1480825665083685 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 1694 0.1479079408403162 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 1684 0.14703481250005457 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 1652 0.14424080181121743 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 1614 0.1409229141182233 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 1614 0.1409229141182233 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 1530 0.13358863606002583 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 1490 0.13009612269897938 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 1441 0.12581779383169753 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 1395 0.12180140346649414 No Hit CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT 1359 0.11865814144155234 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 1326 0.11577681791868906 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 1313 0.11464175107634897 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 1301 0.11359399706803504 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 1282 0.11193505322153798 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 1229 0.10730747301815147 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 1218 0.1063470318438637 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 1207 0.10538659066957592 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 1161 0.10137020030437255 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 1153 0.10067169763216327 No Hit TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC 1147 0.1001478206280063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 8.731283402616067E-5 8 0.0 0.0 0.0 0.0 8.731283402616067E-5 9 0.0 0.0 0.0 0.0 8.731283402616067E-5 10-11 0.0 0.0 0.0 0.0 8.731283402616067E-5 12-13 0.0 0.0 0.0 0.0 8.731283402616067E-5 14-15 0.0 0.0 0.0 0.0 8.731283402616067E-5 16-17 0.0 0.0 0.0 0.0 8.731283402616067E-5 18-19 0.0 4.3656417013080334E-5 0.0 0.0 8.731283402616067E-5 20-21 0.0 8.731283402616067E-5 0.0 0.0 8.731283402616067E-5 22-23 0.0 1.30969251039241E-4 0.0 8.731283402616067E-5 8.731283402616067E-5 24-25 0.0 1.7462566805232134E-4 0.0 8.731283402616067E-5 8.731283402616067E-5 26-27 0.0 1.7462566805232134E-4 0.0 1.7462566805232134E-4 8.731283402616067E-5 28-29 0.0 1.7462566805232134E-4 0.0 6.54846255196205E-4 8.731283402616067E-5 30-31 0.0 1.7462566805232134E-4 0.0 0.0025320721867586595 8.731283402616067E-5 32-33 0.0 1.7462566805232134E-4 0.0 0.01047754008313928 8.731283402616067E-5 34-35 4.3656417013080334E-5 1.7462566805232134E-4 0.0 0.02322521385095874 8.731283402616067E-5 36-37 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.045489986527629714 8.731283402616067E-5 38-39 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.0750453808454851 8.731283402616067E-5 40-41 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.10835522702646538 8.731283402616067E-5 42-43 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.14520124298550519 1.30969251039241E-4 44-45 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.1895561626707948 1.7462566805232134E-4 46-47 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.2491035154766364 1.7462566805232134E-4 48-49 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.31340941773690373 1.7462566805232134E-4 50-51 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.37579443764859555 1.7462566805232134E-4 52-53 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.4381794575602873 1.7462566805232134E-4 54-55 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.5113039560571969 1.7462566805232134E-4 56-57 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.602109303444404 1.7462566805232134E-4 58-59 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.7084563352882678 1.7462566805232134E-4 60-61 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.8210898911820149 2.1828208506540167E-4 62-63 8.731283402616067E-5 1.7462566805232134E-4 0.0 0.936342832096547 2.61938502078482E-4 64-65 8.731283402616067E-5 1.7462566805232134E-4 0.0 1.061287497587983 2.61938502078482E-4 66-67 8.731283402616067E-5 1.7462566805232134E-4 0.0 1.2141722699677904 2.61938502078482E-4 68-69 8.731283402616067E-5 1.7462566805232134E-4 0.0 1.380808813706718 2.61938502078482E-4 70-71 8.731283402616067E-5 1.7462566805232134E-4 0.0 1.5577919282777457 2.61938502078482E-4 72-73 8.731283402616067E-5 1.7462566805232134E-4 0.0 1.7489633783780243 2.61938502078482E-4 74-75 8.731283402616067E-5 1.7462566805232134E-4 0.0 1.9356382175259559 2.61938502078482E-4 76-77 8.731283402616067E-5 1.7462566805232134E-4 0.0 2.151912107408756 2.61938502078482E-4 78-79 8.731283402616067E-5 1.7462566805232134E-4 0.0 2.3914548675595277 2.61938502078482E-4 80-81 8.731283402616067E-5 1.7462566805232134E-4 0.0 2.667057828163104 3.0559491909156237E-4 82-83 8.731283402616067E-5 2.1828208506540167E-4 0.0 2.9616513301673697 3.492513361046427E-4 84-85 8.731283402616067E-5 2.61938502078482E-4 0.0 3.256375801422675 3.92907753117723E-4 86-87 8.731283402616067E-5 2.61938502078482E-4 0.0 3.5703527525807495 4.3656417013080334E-4 88-89 1.7462566805232134E-4 2.61938502078482E-4 0.0 3.9158932932392796 4.3656417013080334E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1000 0.0 52.24849 2 ACCTGGG 1015 0.0 51.94431 3 GTACCTG 1035 0.0 50.96725 1 CCTGGGG 960 0.0 47.498627 4 CTGGGGG 945 0.0 42.221 5 TAACGCA 165 0.0 37.42316 4 ATAACGC 155 3.6379788E-11 33.708702 3 GTACACG 140 1.6976628E-8 30.550837 1 CGTTAAC 205 1.8189894E-12 30.136868 1 TGGGGGG 2180 0.0 29.414286 6 TATTCTC 1515 0.0 28.530525 5 TCTCTGC 1520 0.0 28.436678 8 GCTATTG 2710 0.0 27.342382 8 CTATTGA 2770 0.0 27.264553 9 AGGGCTA 2720 0.0 27.24186 5 GCGCGAG 35 0.008392678 27.143255 40-41 GGGGGGA 2230 0.0 26.41179 7 ATTTAGG 3000 0.0 25.345879 1 AGCACCG 225 1.0186341E-10 25.3326 5 CTCTGCA 1740 0.0 25.114214 9 >>END_MODULE