##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931079_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3506630 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.258629510384615 33.0 33.0 33.0 27.0 33.0 2 31.198795424666987 33.0 33.0 33.0 27.0 33.0 3 31.20745758748428 33.0 33.0 33.0 27.0 33.0 4 34.85402908205314 37.0 37.0 37.0 33.0 37.0 5 34.839727886888554 37.0 37.0 37.0 33.0 37.0 6 34.93099585642055 37.0 37.0 37.0 33.0 37.0 7 34.89161873365596 37.0 37.0 37.0 33.0 37.0 8 34.89508616534964 37.0 37.0 37.0 33.0 37.0 9 34.905350721347844 37.0 37.0 37.0 33.0 37.0 10-11 34.813881418912175 37.0 37.0 37.0 33.0 37.0 12-13 34.7370612240242 37.0 37.0 37.0 33.0 37.0 14-15 36.418328993934345 40.0 37.0 40.0 33.0 40.0 16-17 36.37430039667716 40.0 37.0 40.0 33.0 40.0 18-19 36.311108813875435 40.0 37.0 40.0 33.0 40.0 20-21 36.22532958994819 40.0 37.0 40.0 33.0 40.0 22-23 36.147207432777336 40.0 37.0 40.0 33.0 40.0 24-25 36.03239848515527 40.0 37.0 40.0 33.0 40.0 26-27 35.85916806164323 40.0 37.0 40.0 27.0 40.0 28-29 35.75861026113391 40.0 37.0 40.0 27.0 40.0 30-31 35.628529670937624 40.0 37.0 40.0 27.0 40.0 32-33 35.48163122428086 37.0 37.0 40.0 27.0 40.0 34-35 35.38679173451433 38.5 37.0 40.0 27.0 40.0 36-37 35.193471081921956 37.0 37.0 40.0 27.0 40.0 38-39 35.01756658101938 37.0 37.0 40.0 27.0 40.0 40-41 34.80016825270987 37.0 37.0 40.0 27.0 40.0 42-43 34.63727738598027 37.0 37.0 40.0 24.5 40.0 44-45 34.3921550035219 37.0 33.0 40.0 22.0 40.0 46-47 34.23978919931672 37.0 35.0 40.0 22.0 40.0 48-49 34.24779389328215 37.0 33.0 40.0 22.0 40.0 50-51 33.45599022423238 37.0 33.0 38.5 22.0 40.0 52-53 33.57474911809914 37.0 33.0 38.5 22.0 40.0 54-55 34.09801319215315 37.0 33.0 40.0 22.0 40.0 56-57 34.0655776343669 37.0 33.0 40.0 22.0 40.0 58-59 33.92333351394359 37.0 33.0 40.0 22.0 40.0 60-61 33.948386342442745 37.0 33.0 40.0 22.0 40.0 62-63 33.7892398114429 37.0 33.0 40.0 22.0 40.0 64-65 33.57980439909542 37.0 33.0 40.0 18.5 40.0 66-67 33.36224580295041 37.0 33.0 40.0 15.0 40.0 68-69 33.16223097389802 37.0 33.0 40.0 15.0 40.0 70-71 32.943743280585636 37.0 33.0 40.0 15.0 40.0 72-73 32.348007060910334 37.0 33.0 38.5 6.0 40.0 74-75 32.158023515455014 37.0 33.0 37.0 6.0 40.0 76-77 31.79776452035145 37.0 33.0 37.0 6.0 40.0 78-79 31.543019366172082 37.0 33.0 37.0 6.0 38.5 80-81 31.271981218434796 37.0 33.0 37.0 2.0 37.0 82-83 31.00358748998326 37.0 33.0 37.0 2.0 37.0 84-85 30.624457242423638 37.0 33.0 37.0 2.0 37.0 86-87 30.454042485235114 37.0 33.0 37.0 2.0 37.0 88-89 30.254753281640777 37.0 33.0 37.0 2.0 37.0 90-91 29.98248745946963 33.0 33.0 37.0 2.0 37.0 92-93 29.700961892187085 33.0 33.0 37.0 2.0 37.0 94-95 29.46355004662596 33.0 30.0 37.0 2.0 37.0 96-97 29.177724054148854 33.0 27.0 37.0 2.0 37.0 98-99 28.865107810062653 33.0 27.0 37.0 2.0 37.0 100-101 28.182837795832466 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.00788699911781876 1101 2 0.04619898918629417 1101 3 0.01331077473288289 1101 4 0.06756201461246292 1101 5 0.023901524385131268 1101 6 -0.05603374886283774 1101 7 -0.0920403930400795 1101 8 -0.06354581155378014 1101 9 0.05020912863481897 1101 10-11 -0.004888863407487065 1101 12-13 0.04535893921447354 1101 14-15 0.23911824225964295 1101 16-17 0.23216038014740548 1101 18-19 0.18292451975867863 1101 20-21 0.16008013472826121 1101 22-23 0.13729067325966327 1101 24-25 0.026832526099390464 1101 26-27 0.10347541474973099 1101 28-29 0.1933179833645795 1101 30-31 0.13103830885967938 1101 32-33 0.10110508496634907 1101 34-35 0.06334936297531613 1101 36-37 0.13868751663134304 1101 38-39 0.13733537170008958 1101 40-41 0.17064508333677253 1101 42-43 0.0962542551952339 1101 44-45 -0.00708863448423358 1101 46-47 0.11746371126698563 1101 48-49 0.13729492819815192 1101 50-51 0.11538787868587974 1101 52-53 0.3530576449930152 1101 54-55 0.35963594983364544 1101 56-57 0.3077882215562937 1101 58-59 0.3415749481409591 1101 60-61 0.36516071201447176 1101 62-63 0.40147175117960643 1101 64-65 0.4514852933432465 1101 66-67 0.39175708079084615 1101 68-69 0.3157277084346859 1101 70-71 0.2720423830620504 1101 72-73 0.168065519909824 1101 74-75 0.28817326690567313 1101 76-77 0.16867319173570294 1101 78-79 0.12223697166578162 1101 80-81 0.2920536512863059 1101 82-83 0.27357422607226667 1101 84-85 0.27653271640793164 1101 86-87 0.27269950961958855 1101 88-89 0.20975390092239365 1101 90-91 0.13287136421905998 1101 92-93 0.17111102381166887 1101 94-95 0.25996638794625326 1101 96-97 0.07057886045117456 1101 98-99 0.07695746894375333 1101 100-101 0.3005726487892879 1102 1 0.0922126856523775 1102 2 0.08828928407222847 1102 3 0.049282623300296535 1102 4 0.20651841386251846 1102 5 0.09874749785124948 1102 6 0.12504715877064143 1102 7 0.16740926362039232 1102 8 0.15048957734942547 1102 9 0.07862880313691534 1102 10-11 0.15223449811520595 1102 12-13 0.1852357625256431 1102 14-15 0.32449934114454493 1102 16-17 0.32814827059376483 1102 18-19 0.3011320017444561 1102 20-21 0.3093715914154842 1102 22-23 0.32839082143042475 1102 24-25 0.30255221916585384 1102 26-27 0.22931827640714886 1102 28-29 0.2722494330795371 1102 30-31 0.27551016684086704 1102 32-33 0.31141676992845646 1102 34-35 0.28034032231165895 1102 36-37 0.3991658051304867 1102 38-39 0.4129037358353571 1102 40-41 0.3327961081929729 1102 42-43 0.3056066969356195 1102 44-45 0.4444364574721291 1102 46-47 0.33268427394337863 1102 48-49 0.3366446288098004 1102 50-51 0.24594881201389995 1102 52-53 0.33853462264957557 1102 54-55 0.2913077994740263 1102 56-57 0.22335963453932806 1102 58-59 0.3304752494019425 1102 60-61 0.4050150839832085 1102 62-63 0.486743883111707 1102 64-65 0.5361619788286802 1102 66-67 0.4984938297919328 1102 68-69 0.4719733115447582 1102 70-71 0.5168335299045523 1102 72-73 0.2645717752101362 1102 74-75 0.3052180163383582 1102 76-77 0.262660048084161 1102 78-79 0.30331357730623054 1102 80-81 0.2750667510069107 1102 82-83 0.2713727190992934 1102 84-85 0.323056847754561 1102 86-87 0.31366354273096775 1102 88-89 0.39370130242750534 1102 90-91 0.43917427999858916 1102 92-93 0.4447794759910195 1102 94-95 0.4191277300951981 1102 96-97 0.38699424348747513 1102 98-99 0.38710839095701743 1102 100-101 0.4223453695377053 1103 1 0.05091679026133633 1103 2 -0.01059907538698468 1103 3 -0.03804738861520107 1103 4 -0.05782588684922274 1103 5 -0.06790475145245978 1103 6 -0.09726743608290178 1103 7 -0.027108845777036095 1103 8 -0.10009865581749722 1103 9 -0.1848286632156686 1103 10-11 -0.18819136889884902 1103 12-13 -0.030274455578130244 1103 14-15 0.05740289431553691 1103 16-17 0.027403601901433206 1103 18-19 0.03629256215830168 1103 20-21 0.03240705441696434 1103 22-23 0.011861376654970002 1103 24-25 0.0026357637397254052 1103 26-27 -3.943289264398686E-4 1103 28-29 0.024242050628394907 1103 30-31 0.09090692077397478 1103 32-33 -0.04848419468353171 1103 34-35 -0.015131065027873092 1103 36-37 -0.011476188146552602 1103 38-39 0.09297284020688323 1103 40-41 0.1738429686194891 1103 42-43 0.0012126272966099805 1103 44-45 0.09330381897004258 1103 46-47 0.013180998251897336 1103 48-49 -0.007191111081176871 1103 50-51 0.10107448907086791 1103 52-53 0.228248980906109 1103 54-55 0.21475093601463868 1103 56-57 0.2343391939542272 1103 58-59 0.24189401974267355 1103 60-61 0.19997877078555604 1103 62-63 0.33015224097596985 1103 64-65 0.3561730502214644 1103 66-67 0.3750222962803136 1103 68-69 0.3381557736934653 1103 70-71 0.2914901944424031 1103 72-73 0.19415123643471333 1103 74-75 0.2746009141864434 1103 76-77 0.2933174046554363 1103 78-79 0.2665868440037471 1103 80-81 0.23040827930257635 1103 82-83 0.22017547368161416 1103 84-85 0.31468131151668643 1103 86-87 0.23589186180062782 1103 88-89 0.2780294195303732 1103 90-91 0.3509514859669416 1103 92-93 0.1522923400377607 1103 94-95 0.15807487052306968 1103 96-97 0.21929304775992264 1103 98-99 0.23043985621883678 1103 100-101 0.2747986480032196 1104 1 -0.0681836543342591 1104 2 -0.0969386218657533 1104 3 0.06625248542860263 1104 4 0.011771548394186482 1104 5 -0.11669851728442637 1104 6 -0.15570146083001646 1104 7 -0.027754411641794263 1104 8 -0.11612291359612215 1104 9 -0.021443817349677374 1104 10-11 -0.07169238074576612 1104 12-13 -0.18120794310107868 1104 14-15 -0.14840866933143815 1104 16-17 -0.14063527339691007 1104 18-19 0.025897723025998687 1104 20-21 -0.04950640172993559 1104 22-23 -0.06531020180110403 1104 24-25 -0.05714432592559149 1104 26-27 -0.030366011204002064 1104 28-29 -0.07095918331900464 1104 30-31 -0.1821591888298002 1104 32-33 -0.15995471085281565 1104 34-35 -0.003325272549588476 1104 36-37 -0.10384134488148078 1104 38-39 -0.0715373064239202 1104 40-41 0.03969114422775277 1104 42-43 -0.01080980374048579 1104 44-45 -0.033795884453518 1104 46-47 -0.04123788125813377 1104 48-49 -0.03480703401076113 1104 50-51 -0.05889171872205878 1104 52-53 -0.09089746406904453 1104 54-55 0.024059093534859244 1104 56-57 0.058254332984972734 1104 58-59 -0.049312239537670166 1104 60-61 -0.061966305353188034 1104 62-63 -0.042288365708849085 1104 64-65 -0.0060518219478851165 1104 66-67 -0.017231657743344897 1104 68-69 -0.04266790664541986 1104 70-71 0.045995702469916466 1104 72-73 0.0956366119194243 1104 74-75 -0.04337085741920532 1104 76-77 -0.07857821995420622 1104 78-79 -0.03710969770762773 1104 80-81 0.07690983788046069 1104 82-83 -0.06028576420483134 1104 84-85 0.02298679708661311 1104 86-87 -0.005870850501569436 1104 88-89 0.03164057040264723 1104 90-91 0.013192914326705107 1104 92-93 0.06595548416415298 1104 94-95 0.04816548831112755 1104 96-97 0.03362851133504918 1104 98-99 0.06600585371405998 1104 100-101 0.11789035781822577 1105 1 0.05324267537745442 1105 2 0.05155643888477357 1105 3 0.08900964771395792 1105 4 0.1487964912304065 1105 5 0.056272355341221214 1105 6 0.04166676358078547 1105 7 0.05540207513954698 1105 8 0.018129772727910165 1105 9 0.01626559636664382 1105 10-11 0.004703461616706761 1105 12-13 -0.04260819417336137 1105 14-15 0.07275537950810218 1105 16-17 -0.1072262198497711 1105 18-19 0.009191328155047529 1105 20-21 0.0769637909082661 1105 22-23 0.08121588431070847 1105 24-25 -0.010075744334564263 1105 26-27 0.0710287103000411 1105 28-29 -0.09197527884603574 1105 30-31 -0.15569385324479157 1105 32-33 -0.093596155714053 1105 34-35 0.0024004459206636852 1105 36-37 -0.11033858756974979 1105 38-39 -0.071471923015622 1105 40-41 -0.019549959892522395 1105 42-43 -0.13851107255222672 1105 44-45 -0.0500371230768053 1105 46-47 -0.04306674859333981 1105 48-49 -0.04340588625173325 1105 50-51 -0.11220721795240252 1105 52-53 -0.10839406413181507 1105 54-55 -0.03228272345137384 1105 56-57 -0.03460505158902549 1105 58-59 0.030698371255446943 1105 60-61 -0.04226535038180401 1105 62-63 -0.10918680099048572 1105 64-65 -0.014239692765208645 1105 66-67 -0.11730189820483616 1105 68-69 -0.05670105416533744 1105 70-71 -0.061759890613636514 1105 72-73 -0.02816406956250006 1105 74-75 -0.0341816864061002 1105 76-77 0.05933967342973645 1105 78-79 -0.01944496888064151 1105 80-81 -0.06539995244429164 1105 82-83 0.028295677368550542 1105 84-85 0.09210266395851718 1105 86-87 0.07620027170188592 1105 88-89 0.07136752928955659 1105 90-91 0.12459129730161678 1105 92-93 0.13275864647939883 1105 94-95 0.14261835995042205 1105 96-97 0.0887934269717725 1105 98-99 0.024184757663967105 1105 100-101 0.1506306325032618 1106 1 0.14668312526496052 1106 2 0.1290662950935335 1106 3 0.08795625644649263 1106 4 0.136316961428804 1106 5 0.1144869528137562 1106 6 0.09438943061878291 1106 7 0.0948027185199507 1106 8 0.12455963189024999 1106 9 0.18679722642662 1106 10-11 0.11806103101307741 1106 12-13 0.10848926470669795 1106 14-15 0.13152419379935765 1106 16-17 0.06670446621249226 1106 18-19 0.11391251368508648 1106 20-21 0.23233461969969227 1106 22-23 0.2125682205516526 1106 24-25 0.228099460333695 1106 26-27 0.3218291426818851 1106 28-29 0.2656172134084187 1106 30-31 0.29295713665809586 1106 32-33 0.2977789493553473 1106 34-35 0.27377719973240744 1106 36-37 0.2674275654692835 1106 38-39 0.23248801726551704 1106 40-41 0.4380441929050889 1106 42-43 0.4490038091176416 1106 44-45 0.3883721507449067 1106 46-47 0.35585868610652227 1106 48-49 0.31944761978707703 1106 50-51 0.1561812746351734 1106 52-53 0.335598638581736 1106 54-55 0.4423757441218399 1106 56-57 0.38970267049303686 1106 58-59 0.4897893309212762 1106 60-61 0.35915085692411 1106 62-63 0.6156672650812638 1106 64-65 0.6672227366448453 1106 66-67 0.59823892300372 1106 68-69 0.5878042169212847 1106 70-71 0.6139678908973139 1106 72-73 0.3538645797803426 1106 74-75 0.5286034086258553 1106 76-77 0.5113954933047999 1106 78-79 0.5581080024464171 1106 80-81 0.5504794088416887 1106 82-83 0.6492830211637006 1106 84-85 0.6954862271773372 1106 86-87 0.6500686073183992 1106 88-89 0.7003446987597073 1106 90-91 0.6635622224551163 1106 92-93 0.6112541909976343 1106 94-95 0.6877869042390472 1106 96-97 0.8202326424940907 1106 98-99 0.8990281439986916 1106 100-101 0.7992961121203912 1107 1 -0.17851152423151007 1107 2 -0.15556787140409511 1107 3 -0.17652467361649826 1107 4 -0.21325087734594206 1107 5 -0.2072419451484322 1107 6 -0.21107973486857645 1107 7 -0.1948245026353348 1107 8 -0.17727810609103045 1107 9 -0.2840904211446045 1107 10-11 -0.3120356897493579 1107 12-13 -0.3134725285953195 1107 14-15 -0.26700793290678604 1107 16-17 -0.19831172772408223 1107 18-19 -0.2154350669909988 1107 20-21 -0.14101819934221282 1107 22-23 -0.15631154495556387 1107 24-25 -0.2827071602204825 1107 26-27 -0.20317752982891335 1107 28-29 -0.2489449136970876 1107 30-31 -0.25285468153486335 1107 32-33 -0.19131601302751733 1107 34-35 -0.23305284163122053 1107 36-37 -0.13729202498287663 1107 38-39 -0.1851903997420905 1107 40-41 -0.17968776235210981 1107 42-43 -0.17789208350218644 1107 44-45 -0.0560419000009702 1107 46-47 -0.37584932244852354 1107 48-49 -0.22896574611205978 1107 50-51 -0.3404734942033514 1107 52-53 -0.17911781628460943 1107 54-55 -0.09744623526711393 1107 56-57 -0.08747935916019856 1107 58-59 -0.021935071251412808 1107 60-61 -0.02684078494058184 1107 62-63 0.0070235265456233265 1107 64-65 -0.006452537659605184 1107 66-67 0.010023901689244497 1107 68-69 -0.06796852309436474 1107 70-71 -0.12709970292214479 1107 72-73 -0.19013654634176902 1107 74-75 -0.06073690445032298 1107 76-77 -0.0330726888027435 1107 78-79 -0.11968967753640669 1107 80-81 0.03680580315920068 1107 82-83 0.11063860648030399 1107 84-85 0.18173180008998813 1107 86-87 0.1224171294610521 1107 88-89 -0.046129586075640105 1107 90-91 0.1103557992886337 1107 92-93 0.03164641205604113 1107 94-95 0.12976702074998414 1107 96-97 0.13507550931314682 1107 98-99 0.06605237816250309 1107 100-101 0.10240528467764776 1108 1 -0.12098000638606621 1108 2 -0.1678598221982952 1108 3 -0.09647507784985265 1108 4 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-0.7583692178077825 2316 74-75 -1.025745448388271 2316 76-77 -0.9943324936048477 2316 78-79 -1.0568551167316684 2316 80-81 -1.0597314838756624 2316 82-83 -1.0390599375958232 2316 84-85 -1.0863986204744158 2316 86-87 -0.9991163577092266 2316 88-89 -0.953474609174215 2316 90-91 -0.995154695246466 2316 92-93 -0.981290629945132 2316 94-95 -0.83678864381179 2316 96-97 -0.9040009163269573 2316 98-99 -0.990652195032105 2316 100-101 -1.0608281467612777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89714.0 3 14551.0 4 10663.0 5 9072.0 6 9272.0 7 9953.0 8 9928.0 9 10381.0 10 10586.0 11 11013.0 12 11404.0 13 12179.0 14 12604.0 15 14200.0 16 16922.0 17 21079.0 18 18065.0 19 14406.0 20 14548.0 21 15435.0 22 16927.0 23 19829.0 24 22712.0 25 26401.0 26 29607.0 27 35478.0 28 42272.0 29 51838.0 30 63486.0 31 79404.0 32 102574.0 33 135848.0 34 187928.0 35 273710.0 36 431577.0 37 793795.0 38 856843.0 39 426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.027227389597265 24.762021276353043 13.404889500304568 22.805861833745126 2 13.78523036698786 21.951625727365126 44.24073974787338 20.02240415777364 3 16.472301294748856 32.21983182694704 30.15419950687641 21.15366737142769 4 10.650363286995871 18.644730470650437 44.293322808400404 26.41158343395329 5 11.471917088043192 39.16918076190275 37.008831724968296 12.350070425085761 6 24.554914547585575 42.46393260766035 21.28000387836755 11.70114896638653 7 23.306051679247595 33.09411029963241 28.097546647350875 15.502291373769117 8 22.77271910637849 37.84488240846625 22.321174460949685 17.061224024205575 9 25.82268445772722 15.5049149753467 24.389656165606294 34.28274440131979 10-11 22.30590503268454 28.058455025519535 31.022599349374865 18.613040592421065 12-13 22.717508101800362 26.028010384905386 30.766194760322453 20.488286752971803 14-15 20.742441392397374 27.359996395264908 27.972387843259046 23.925174369078675 16-17 19.154083814196003 29.56588535127394 29.72477786347678 21.55525297105327 18-19 18.911876020068995 30.379184047468378 31.121818518335786 19.587121414126837 20-21 19.7179485717056 28.71710160467457 30.374319503340814 21.190630320279013 22-23 20.145096574203723 29.730667335875182 29.36987934284484 20.75435674707625 24-25 19.945334971943858 29.69697825219345 30.08631222659822 20.271374549264472 26-27 20.05579430963632 29.144306641989605 29.759084933397595 21.040814114976484 28-29 19.694236346577767 30.332712604409362 29.025246461702547 20.947804587310323 30-31 21.164636702475025 29.338966472082884 28.692719220448122 20.80367760499397 32-33 20.87301768364498 29.768324573736038 28.732572298759777 20.626085443859203 34-35 21.1545843159957 28.705651865181096 29.911524740277702 20.2282390785455 36-37 21.257189951605955 29.190319480526888 29.71053404550808 19.841956522359077 38-39 21.735726324134568 28.466604688832298 30.529454205319638 19.268214781713496 40-41 20.589063140306944 28.636601238971217 30.535809564711762 20.238526056010077 42-43 21.220422804921764 28.697575363689744 29.464437213061316 20.617564618327176 44-45 20.39649695810155 30.122173569654677 29.52942151100657 19.9519079612372 46-47 21.605568886801606 28.76922964201881 28.739634802554292 20.885566668625287 48-49 21.426833693240134 27.987057978039164 29.558906657480122 21.027201671240586 50-51 21.448453687958658 28.361539822897342 28.229967130452415 21.96003935869159 52-53 20.131302801327706 29.06525060387813 29.095943538736556 21.707503056057607 54-55 20.748187777499254 29.805723338037733 28.351781775560266 21.094307108902754 56-57 21.3761384828848 28.685656649623958 28.874925250130477 21.063279617360763 58-59 21.1500399195259 27.964750672453064 29.98797408191527 20.89723532610577 60-61 21.105726319173137 30.173987946848996 28.219547748020336 20.50073798595753 62-63 21.41349358787836 29.228376310302963 27.497906332078255 21.860223769740426 64-65 20.156164637006796 29.182649943258742 28.145722587081067 22.515462832653395 66-67 21.134059306885923 27.381267068839488 29.17576206564928 22.30891155862531 68-69 20.109477428731186 28.676985984647946 28.36467930034155 22.848857286279316 70-71 20.75163408036056 28.136859626621714 29.215644285965066 21.895862007052664 72-73 21.68916025810429 27.942314455548495 29.40960037774165 20.958924908605557 74-75 21.30253302091987 29.132321401995092 28.216100381417036 21.349045195668 76-77 20.627637682600792 28.969872421086045 29.486089338965364 20.916400557347796 78-79 20.350703361498702 28.933084564512896 28.9582705472542 21.757941526734207 80-81 20.46203723543182 29.00941999611517 29.815650827643164 20.712891940809847 82-83 21.64140076899547 27.820807209286063 30.02733255929455 20.510459462423917 84-85 22.64569024724047 28.150935043233442 28.380525796571664 20.822848912954417 86-87 21.021110013693654 29.405789189203873 28.83435538970876 20.738745407393715 88-89 21.79121696820499 28.32298660536543 28.80451867879253 21.08127774763705 90-91 21.540603298682473 27.513140442423612 30.01293777586065 20.93331848303327 92-93 21.549682584302982 28.627450251818882 28.763045584355428 21.0598215795227 94-95 20.181261052546763 28.439743993800715 29.49455948252437 21.884435471128157 96-97 20.950260530016646 28.422590997005702 28.41466542370829 22.212483049269363 98-99 20.10045392641205 29.299775950240615 28.86814311763926 21.73162700570807 100-101 20.774279197273138 28.001899938477635 28.739197170314117 22.484623693935106 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 63.0 1 50.0 2 53.0 3 74.0 4 62.0 5 50.5 6 70.0 7 125.0 8 227.0 9 284.0 10 261.5 11 291.0 12 414.0 13 571.0 14 712.5 15 884.0 16 1162.5 17 1396.0 18 1535.0 19 1749.0 20 2035.0 21 2252.5 22 2505.0 23 2858.0 24 3217.0 25 3697.5 26 4869.0 27 7123.5 28 13336.0 29 30636.5 30 54079.5 31 75536.5 32 104077.0 33 139175.5 34 184781.5 35 216170.5 36 231918.5 37 257462.5 38 243719.0 39 186579.0 40 137571.5 41 104104.0 42 85295.0 43 77520.5 44 72754.5 45 76560.5 46 81933.0 47 86057.5 48 89610.5 49 88151.5 50 85766.0 51 85571.0 52 82742.0 53 79151.5 54 75982.0 55 68945.5 56 61889.0 57 51705.0 58 42045.0 59 39065.5 60 33755.0 61 26566.0 62 20929.0 63 17135.0 64 13822.0 65 9537.5 66 6854.0 67 5496.0 68 4669.5 69 3855.0 70 3095.0 71 2491.5 72 1919.5 73 1520.5 74 1260.5 75 1026.0 76 842.5 77 662.5 78 521.5 79 425.5 80 365.0 81 303.0 82 227.5 83 178.5 84 147.5 85 120.0 86 96.0 87 77.0 88 63.0 89 48.0 90 43.5 91 37.5 92 23.5 93 17.0 94 10.0 95 8.0 96 9.5 97 8.0 98 4.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.992437183278532E-4 2 0.003935402366374553 3 1.1406963380795807E-4 4 0.0010266267042716225 5 0.0019106663662832974 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.129352112997379E-5 12-13 5.7034816903979035E-5 14-15 0.0037357805072106265 16-17 0.004890735549516202 18-19 0.003621710873402669 20-21 0.0 22-23 0.0 24-25 1.4258704225994759E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.136914929718847E-4 42-43 2.8517408451989518E-5 44-45 5.846068732657851E-4 46-47 0.7498224791323863 48-49 0.5395921440243197 50-51 0.4176089293709345 52-53 0.3519190790017766 54-55 0.34295035404362595 56-57 0.30811633961952073 58-59 0.3093283294787303 60-61 0.28377673150574767 62-63 0.2788004437308755 64-65 0.2742091409701052 66-67 0.2626881079555014 68-69 0.24142837995454325 70-71 0.25505970119459426 72-73 0.8304554515303868 74-75 0.5524107191234889 76-77 0.6453346945642968 78-79 0.35997524688946364 80-81 0.31314966221129686 82-83 0.29231769533711854 84-85 0.28441837319591745 86-87 0.25735535257497943 88-89 0.2608629938145741 90-91 0.27110074344883833 92-93 0.22769724778491032 94-95 0.19594311347361998 96-97 0.15131336924625638 98-99 0.16032487031708506 100-101 0.15581911978167073 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3506630.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.023980375382084 #Duplication Level Percentage of deduplicated Percentage of total 1 80.87690134813906 23.473695975501013 2 9.803739642101906 5.690870939554423 3 3.307226711427038 2.879666495081934 4 1.5430213560296795 1.791384862248035 5 0.8742759922551716 1.26874846209409 6 0.5529110247263568 0.9628607238594107 7 0.39101723827841245 0.7944213655160121 8 0.2783576473144677 0.6463237514394111 9 0.21525507192705629 0.5622803084981107 >10 1.565219133905028 9.243926734024464 >50 0.24717950686712706 5.092206428628976 >100 0.2768722708616753 17.041452762630797 >500 0.038701071142994904 7.69762031162682 >1k 0.026360168355050097 14.734179701969255 >5k 0.00187581722376761 3.5725657773287067 >10k+ 0.0010859994453391426 4.5477953999985266 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 21904 0.6246453147323784 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 18763 0.5350721347846793 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 15497 0.4419342787804815 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 14295 0.40765635382119014 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 14227 0.40571717004645486 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 14226 0.4056886526380029 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 13927 0.397161947510858 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 13005 0.37086889691812364 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 11788 0.3361632108320524 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 10885 0.3104119909999059 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 10194 0.29070646175958115 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 9474 0.27017392767414866 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 9389 0.26774994795572954 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 8739 0.24921363246193637 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 7924 0.22597194457356493 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 7357 0.20980257398128688 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 7102 0.20253063482602954 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 6608 0.18844303505074672 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 6497 0.1852776027125759 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 6447 0.18385173228997642 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 6127 0.17472616158533977 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 6112 0.17429840045855993 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 5872 0.16745422243008246 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 5785 0.16497320789475936 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 5440 0.15513470197882295 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 5328 0.15194075223220013 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 5298 0.15108522997864046 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 5094 0.1452676786544346 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 5071 0.14461177826003885 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 5013 0.14295776856982345 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 4925 0.14044823662604836 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 4909 0.13999195809081655 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 4825 0.1375964957808494 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 4794 0.13671245611883773 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 4651 0.13263446671020324 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 4646 0.1324918796679433 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 4587 0.1308093525692759 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 4519 0.12887016879454063 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 4477 0.12767243763955707 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 4456 0.1270735720620653 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 4444 0.1267313631606414 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 4418 0.12598991054088968 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 4406 0.12564770163946581 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 4387 0.125105870878878 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 4369 0.1245925575267422 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 4316 0.12308113487878676 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 4182 0.11925980214622016 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 3988 0.11372742490653419 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 3921 0.1118167585402509 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 3908 0.11144603223037504 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 3627 0.10343264045536597 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 3596 0.1025486007933543 No Hit GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT 3564 0.10163604372289063 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 3540 0.10095162592004289 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 3520 0.1003812777510031 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8517408451989518E-5 0.0 5 0.0 0.0 0.0 2.8517408451989518E-5 0.0 6 0.0 0.0 0.0 2.8517408451989518E-5 0.0 7 0.0 0.0 0.0 8.555222535596856E-5 0.0 8 0.0 0.0 0.0 8.555222535596856E-5 0.0 9 0.0 0.0 0.0 8.555222535596856E-5 0.0 10-11 0.0 0.0 0.0 8.555222535596856E-5 0.0 12-13 0.0 2.8517408451989518E-5 0.0 1.1406963380795807E-4 0.0 14-15 0.0 2.8517408451989518E-5 0.0 1.4258704225994758E-4 0.0 16-17 0.0 2.8517408451989518E-5 0.0 1.4258704225994758E-4 0.0 18-19 0.0 2.8517408451989518E-5 0.0 1.4258704225994758E-4 0.0 20-21 0.0 2.8517408451989518E-5 0.0 1.711044507119371E-4 0.0 22-23 0.0 2.8517408451989518E-5 0.0 1.996218591639266E-4 0.0 24-25 0.0 2.8517408451989518E-5 0.0 2.994327887458899E-4 0.0 26-27 0.0 2.8517408451989518E-5 0.0 6.131242817177746E-4 0.0 28-29 0.0 2.8517408451989518E-5 0.0 0.001425870422599476 0.0 30-31 0.0 2.8517408451989518E-5 0.0 0.0062595711552117 1.4258704225994759E-5 32-33 0.0 2.8517408451989518E-5 0.0 0.022029698029161902 2.8517408451989518E-5 34-35 0.0 2.8517408451989518E-5 0.0 0.04723908710072064 2.8517408451989518E-5 36-37 0.0 2.8517408451989518E-5 0.0 0.0919401248492142 2.8517408451989518E-5 38-39 0.0 2.8517408451989518E-5 0.0 0.15172687166881021 2.8517408451989518E-5 40-41 0.0 2.8517408451989518E-5 0.0 0.214878672685741 2.8517408451989518E-5 42-43 0.0 2.8517408451989518E-5 0.0 0.2893804022665636 2.8517408451989518E-5 44-45 0.0 2.8517408451989518E-5 0.0 0.3767577417634595 2.8517408451989518E-5 46-47 0.0 2.8517408451989518E-5 0.0 0.4853948092613136 2.8517408451989518E-5 48-49 0.0 2.8517408451989518E-5 0.0 0.6006764329284813 2.8517408451989518E-5 50-51 0.0 2.8517408451989518E-5 0.0 0.7219609710747925 2.8517408451989518E-5 52-53 0.0 2.8517408451989518E-5 0.0 0.8403509922632271 2.8517408451989518E-5 54-55 0.0 2.8517408451989518E-5 0.0 0.9712031209451809 2.8517408451989518E-5 56-57 0.0 2.8517408451989518E-5 0.0 1.1282627479945133 4.277611267798428E-5 58-59 0.0 2.8517408451989518E-5 0.0 1.316591713411452 5.7034816903979035E-5 60-61 0.0 2.8517408451989518E-5 0.0 1.5131051750541118 5.7034816903979035E-5 62-63 0.0 2.8517408451989518E-5 0.0 1.7052412144993911 5.7034816903979035E-5 64-65 0.0 2.8517408451989518E-5 0.0 1.912776654508745 5.7034816903979035E-5 66-67 0.0 2.8517408451989518E-5 0.0 2.146519592885477 5.7034816903979035E-5 68-69 0.0 2.8517408451989518E-5 0.0 2.402577403375891 5.7034816903979035E-5 70-71 0.0 2.8517408451989518E-5 0.0 2.671867291388028 5.7034816903979035E-5 72-73 0.0 2.8517408451989518E-5 0.0 2.976433213655276 5.7034816903979035E-5 74-75 0.0 2.8517408451989518E-5 0.0 3.266583585950043 5.7034816903979035E-5 76-77 0.0 2.8517408451989518E-5 0.0 3.594433972218341 5.7034816903979035E-5 78-79 0.0 2.8517408451989518E-5 0.0 3.9553075174740417 5.7034816903979035E-5 80-81 0.0 2.8517408451989518E-5 0.0 4.36809700481659 5.7034816903979035E-5 82-83 0.0 2.8517408451989518E-5 0.0 4.809275572273094 5.7034816903979035E-5 84-85 0.0 2.8517408451989518E-5 0.0 5.236737266264191 5.7034816903979035E-5 86-87 0.0 2.8517408451989518E-5 0.0 5.708899998003782 5.7034816903979035E-5 88-89 0.0 2.8517408451989518E-5 0.0 6.216295987885805 8.555222535596856E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2890 0.0 53.242195 2 ACCTGGG 2925 0.0 51.471336 3 GTACCTG 3250 0.0 48.072975 1 CCTGGGG 2770 0.0 42.55825 4 GTACACG 360 0.0 42.084084 1 CTGGGGG 2665 0.0 38.372555 5 GTATCAA 7865 0.0 29.977886 1 TATTCTC 3050 0.0 28.716805 5 TGGGGGG 7570 0.0 28.580652 6 TATTCGC 525 0.0 27.957052 9 TATCACG 240 0.0 27.61768 2 CAACGCA 8270 0.0 26.90646 5 CTATTGA 6155 0.0 26.846418 9 GCTATTG 6195 0.0 26.824017 8 AGGGCTA 6110 0.0 26.577682 5 CATGGGG 7420 0.0 26.479414 4 TCTCTGC 3280 0.0 26.269726 8 ATCAACG 8475 0.0 26.19976 3 GGTATCA 5195 0.0 25.700048 1 GTACATG 11075 0.0 25.64944 1 >>END_MODULE