##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931078_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3004317 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.304586034030365 33.0 33.0 33.0 27.0 33.0 2 31.226001783433638 33.0 33.0 33.0 27.0 33.0 3 31.23885994720264 33.0 33.0 33.0 27.0 33.0 4 34.87867591868634 37.0 37.0 37.0 33.0 37.0 5 34.85275322144767 37.0 37.0 37.0 33.0 37.0 6 34.951969449295795 37.0 37.0 37.0 33.0 37.0 7 34.91947853705185 37.0 37.0 37.0 33.0 37.0 8 34.92865533164443 37.0 37.0 37.0 33.0 37.0 9 34.938484853628964 37.0 37.0 37.0 33.0 37.0 10-11 34.84520524964576 37.0 37.0 37.0 33.0 37.0 12-13 34.747935720498205 37.0 37.0 37.0 33.0 37.0 14-15 36.40972207659844 40.0 37.0 40.0 33.0 40.0 16-17 36.35253570112608 40.0 37.0 40.0 33.0 40.0 18-19 36.287672705643246 40.0 37.0 40.0 33.0 40.0 20-21 36.20622108119749 40.0 37.0 40.0 33.0 40.0 22-23 36.10857625876364 40.0 37.0 40.0 33.0 40.0 24-25 35.97207052384951 40.0 37.0 40.0 33.0 40.0 26-27 35.78141570946075 40.0 37.0 40.0 27.0 40.0 28-29 35.67132562908641 40.0 37.0 40.0 27.0 40.0 30-31 35.52762957437581 37.0 37.0 40.0 27.0 40.0 32-33 35.3655636206166 37.0 37.0 40.0 27.0 40.0 34-35 35.24192919721854 37.0 37.0 40.0 27.0 40.0 36-37 35.0158065876537 37.0 37.0 40.0 27.0 40.0 38-39 34.80408059469091 37.0 37.0 40.0 27.0 40.0 40-41 34.55789585453199 37.0 37.0 40.0 22.0 40.0 42-43 34.35761622358759 37.0 33.0 40.0 22.0 40.0 44-45 34.03639346313987 37.0 33.0 40.0 22.0 40.0 46-47 33.85144593596481 37.0 33.0 40.0 22.0 40.0 48-49 33.802867340563594 37.0 33.0 40.0 15.0 40.0 50-51 32.97193372070923 37.0 33.0 38.5 15.0 40.0 52-53 33.09313830730912 37.0 33.0 38.5 15.0 40.0 54-55 33.56713256290864 37.0 33.0 40.0 15.0 40.0 56-57 33.47891866936811 37.0 33.0 40.0 15.0 40.0 58-59 33.29066889412802 37.0 33.0 40.0 15.0 40.0 60-61 33.294358584663335 37.0 33.0 40.0 15.0 40.0 62-63 33.10786195331585 37.0 33.0 40.0 15.0 40.0 64-65 32.87406405515796 37.0 33.0 40.0 15.0 40.0 66-67 32.63966202634409 37.0 33.0 40.0 10.5 40.0 68-69 32.407179402173604 37.0 33.0 40.0 6.0 40.0 70-71 32.197963796763126 37.0 33.0 37.0 6.0 40.0 72-73 31.617518890316834 37.0 33.0 37.0 2.0 40.0 74-75 31.42592076668341 37.0 33.0 37.0 2.0 40.0 76-77 31.0848572237883 37.0 33.0 37.0 2.0 38.5 78-79 30.83722972642368 37.0 33.0 37.0 2.0 37.0 80-81 30.60789074521763 37.0 33.0 37.0 2.0 37.0 82-83 30.36364305098297 37.0 33.0 37.0 2.0 37.0 84-85 30.000134140305434 35.0 30.0 37.0 2.0 37.0 86-87 29.841430681249683 33.0 33.0 37.0 2.0 37.0 88-89 29.673923058052797 33.0 30.0 37.0 2.0 37.0 90-91 29.394603998179953 33.0 27.0 37.0 2.0 37.0 92-93 29.1055367992126 33.0 27.0 37.0 2.0 37.0 94-95 28.861050781259102 33.0 27.0 37.0 2.0 37.0 96-97 28.567998982797086 33.0 27.0 37.0 2.0 37.0 98-99 28.240215829421462 33.0 27.0 37.0 2.0 37.0 100-101 27.538371949431436 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18589582186692866 1101 2 0.20685102119213994 1101 3 0.13391392569747396 1101 4 0.19574905117413266 1101 5 0.2651476631449796 1101 6 0.20515279666745556 1101 7 0.14982264557960434 1101 8 0.2072083767021482 1101 9 0.18892121088044433 1101 10-11 0.18167189006052809 1101 12-13 0.17947047987069453 1101 14-15 0.19410588810777796 1101 16-17 0.1489345660807757 1101 18-19 0.2602851771217516 1101 20-21 0.36984828156932537 1101 22-23 0.3488829613104869 1101 24-25 0.34502358808690303 1101 26-27 0.24371568150814227 1101 28-29 0.21315079185131225 1101 30-31 0.2893209839452595 1101 32-33 0.29002310412897003 1101 34-35 0.4165853810359934 1101 36-37 0.42567049009958424 1101 38-39 0.42999221766817897 1101 40-41 0.4049643014925266 1101 42-43 0.2984468401671947 1101 44-45 0.1774099203948225 1101 46-47 0.21627107505511134 1101 48-49 0.23422169156043537 1101 50-51 0.2692067556214468 1101 52-53 0.3863483359769617 1101 54-55 0.4840034540149887 1101 56-57 0.5167117580472222 1101 58-59 0.5095628532169414 1101 60-61 0.3691077904072273 1101 62-63 0.4961884202891511 1101 64-65 0.5696059727716047 1101 66-67 0.5709911953441349 1101 68-69 0.4760196176384781 1101 70-71 0.4739939918713034 1101 72-73 0.41043569561929516 1101 74-75 0.47117277693947557 1101 76-77 0.41025399230792914 1101 78-79 0.428053178314201 1101 80-81 0.5305958707142899 1101 82-83 0.4834674778372907 1101 84-85 0.5325868078871956 1101 86-87 0.43811146402764223 1101 88-89 0.41890842829151254 1101 90-91 0.28450002893866255 1101 92-93 0.19349899649021296 1101 94-95 0.2510805380400818 1101 96-97 0.20129509299431092 1101 98-99 0.1710936602782489 1101 100-101 0.16858859172572238 1102 1 -0.027522269043007697 1102 2 -0.06276785106715721 1102 3 -0.05250156440656539 1102 4 -0.0414085705977314 1102 5 -0.21087569203848489 1102 6 -0.0843090444087835 1102 7 -0.04696003951282535 1102 8 -0.019477783596052234 1102 9 -0.011062926355130287 1102 10-11 -0.003367384285475339 1102 12-13 0.0703994611540324 1102 14-15 0.1698196902278113 1102 16-17 0.23324700499608042 1102 18-19 0.10320790801760182 1102 20-21 0.09747271292550153 1102 22-23 0.0559180082170343 1102 24-25 0.06278032330082794 1102 26-27 0.07766251935007773 1102 28-29 0.10486202639322784 1102 30-31 0.12306202597101645 1102 32-33 0.09021720020155755 1102 34-35 0.025402289979005843 1102 36-37 0.029326908223715975 1102 38-39 0.020585089125169986 1102 40-41 -0.023135172553708117 1102 42-43 -0.08189998402716725 1102 44-45 0.031364529209000125 1102 46-47 -0.1365117134126379 1102 48-49 -1.8031595770651165E-4 1102 50-51 0.05766730743431481 1102 52-53 0.08482888429391267 1102 54-55 0.10781729543547414 1102 56-57 0.045669196315159866 1102 58-59 0.0934477231569133 1102 60-61 0.16279853499342067 1102 62-63 0.08675468405942866 1102 64-65 0.1397314859403096 1102 66-67 0.08061375703291418 1102 68-69 0.11588317749488652 1102 70-71 0.1720915295399621 1102 72-73 0.045577091285402105 1102 74-75 0.0221619527033603 1102 76-77 -0.041742494458951285 1102 78-79 0.041453356776667505 1102 80-81 0.1260482733183821 1102 82-83 0.10968778719175276 1102 84-85 0.21284828378884413 1102 86-87 0.11798330918669464 1102 88-89 0.0701596961350539 1102 90-91 0.03357681222974662 1102 92-93 -0.051310754093911726 1102 94-95 -0.13117471930144475 1102 96-97 -0.12138173449863388 1102 98-99 0.0780004301267212 1102 100-101 -0.004039533515769023 1103 1 0.12952679492647334 1103 2 0.17744615432673072 1103 3 0.13712656354454467 1103 4 0.09359980949763269 1103 5 0.07613680092746478 1103 6 0.13872145087369603 1103 7 0.21598936512971534 1103 8 0.1233251182626347 1103 9 0.016636357660217982 1103 10-11 0.12226762518847067 1103 12-13 0.20079872803241727 1103 14-15 0.24701282620655718 1103 16-17 0.2331254054977947 1103 18-19 0.17611929534572823 1103 20-21 0.26453656815829163 1103 22-23 0.23698130194090083 1103 24-25 0.27807013847688467 1103 26-27 0.3563997441104263 1103 28-29 0.2756761414150546 1103 30-31 0.289948373890212 1103 32-33 0.30209840095558604 1103 34-35 0.264293103231104 1103 36-37 0.23938308342422232 1103 38-39 0.22752975132650022 1103 40-41 0.25990415859435956 1103 42-43 0.23664670446785863 1103 44-45 0.23192135889556198 1103 46-47 0.15867115983584057 1103 48-49 0.16572582211271936 1103 50-51 0.2125001734512395 1103 52-53 0.22937762922422422 1103 54-55 0.41750156212547296 1103 56-57 0.42351766912026534 1103 58-59 0.39729661162446916 1103 60-61 0.41621583943457097 1103 62-63 0.47305544622879836 1103 64-65 0.44467203527105426 1103 66-67 0.4162601546857587 1103 68-69 0.5038530310285623 1103 70-71 0.46055762570383507 1103 72-73 0.2839392718360436 1103 74-75 0.3970978714085369 1103 76-77 0.3902781598326399 1103 78-79 0.4107733354344667 1103 80-81 0.4273210268876717 1103 82-83 0.4188097549523846 1103 84-85 0.46177357757833803 1103 86-87 0.5283126426804579 1103 88-89 0.4835384971068777 1103 90-91 0.24042339267684198 1103 92-93 0.1945684753019279 1103 94-95 0.294316327548092 1103 96-97 0.2582269837174671 1103 98-99 0.2962184171293707 1103 100-101 0.24039235192668684 1104 1 -0.1349967173737454 1104 2 -0.05552584589835874 1104 3 -0.043137505535575826 1104 4 -0.022259609375829825 1104 5 -0.05232728262282649 1104 6 -0.04698512398014287 1104 7 -0.04928062408656331 1104 8 -0.058878642373223045 1104 9 -0.11900251747945845 1104 10-11 -0.13498973823209326 1104 12-13 -0.1118708227148062 1104 14-15 -0.023542908631057458 1104 16-17 0.00395119271512101 1104 18-19 -0.029627256218027753 1104 20-21 -0.006046039848307316 1104 22-23 0.00849023037902441 1104 24-25 0.06278137249510252 1104 26-27 0.0022983702483756474 1104 28-29 -0.08536896072021705 1104 30-31 -0.09278900289803005 1104 32-33 -0.022573981810218413 1104 34-35 -0.06196347581448691 1104 36-37 -0.030361345848241683 1104 38-39 -0.17478047988610967 1104 40-41 -0.09989719815614251 1104 42-43 -0.2585910024928708 1104 44-45 -0.13029823853517541 1104 46-47 -0.18919403102682253 1104 48-49 -0.27836391819370476 1104 50-51 -0.34091157058298194 1104 52-53 -0.15541830121331657 1104 54-55 -0.03835988946205049 1104 56-57 -0.06463941718510569 1104 58-59 -0.1042231005340426 1104 60-61 -0.05911258169784617 1104 62-63 -0.038544001148601126 1104 64-65 -0.03555792555612669 1104 66-67 -0.004007899432664885 1104 68-69 0.06300501028339767 1104 70-71 -0.031188636456562335 1104 72-73 -0.012138910710863371 1104 74-75 -0.1049171568944196 1104 76-77 -0.09366024089224467 1104 78-79 0.07505246267662358 1104 80-81 0.06024111825644596 1104 82-83 -0.07156034830259728 1104 84-85 0.13465935397749362 1104 86-87 0.01616401797804201 1104 88-89 -0.0485789018919256 1104 90-91 0.10584951041118273 1104 92-93 -0.09256530599411406 1104 94-95 -0.010159777725828434 1104 96-97 -0.12885333834712398 1104 98-99 -0.07847733610603314 1104 100-101 -0.02696536095991675 1105 1 0.046452507537633636 1105 2 0.04955485218410871 1105 3 -0.008969713632737353 1105 4 0.05409821464623832 1105 5 0.09440376665224903 1105 6 0.16253940112334675 1105 7 0.08709043249668724 1105 8 0.06364611732713854 1105 9 0.12861859450973867 1105 10-11 0.02176324639154359 1105 12-13 0.07454623431602414 1105 14-15 0.10897702940076925 1105 16-17 0.0542653579340282 1105 18-19 0.017492445487178543 1105 20-21 0.20158333168071607 1105 22-23 0.21276492061925012 1105 24-25 0.1320313435759033 1105 26-27 0.11472622089772955 1105 28-29 0.10859714273376397 1105 30-31 0.10588336256282105 1105 32-33 0.12814294408419613 1105 34-35 0.06472478385507685 1105 36-37 0.0632895112952383 1105 38-39 0.049110401214356614 1105 40-41 0.17850756470100748 1105 42-43 0.14959599608334173 1105 44-45 0.11146724891280257 1105 46-47 0.1403461482830295 1105 48-49 -0.053361674928630976 1105 50-51 -0.04736536310636552 1105 52-53 0.04384963760986693 1105 54-55 0.037604579381429915 1105 56-57 0.09424510169129974 1105 58-59 0.13282347613535705 1105 60-61 0.1141154821427719 1105 62-63 0.029836664176791317 1105 64-65 0.18437989005504818 1105 66-67 0.13798845512646096 1105 68-69 0.10296318284940043 1105 70-71 0.16108285591471372 1105 72-73 0.045355702750239146 1105 74-75 0.1856870760116287 1105 76-77 0.14983656069825813 1105 78-79 0.08379216933322908 1105 80-81 0.14766975269721527 1105 82-83 0.0743066385113309 1105 84-85 0.016979668445419094 1105 86-87 0.034273329311883316 1105 88-89 0.12641858457010713 1105 90-91 0.08014082784021781 1105 92-93 -0.05798610468296772 1105 94-95 0.03720430473551417 1105 96-97 0.030159289883851415 1105 98-99 0.13473436000776573 1105 100-101 0.23070038930143966 1106 1 0.10411593137649433 1106 2 0.04563246253360376 1106 3 0.0842437311422124 1106 4 0.08903972954892936 1106 5 0.13477778850190703 1106 6 0.08201159419213866 1106 7 0.0905848558842024 1106 8 0.05489156984572929 1106 9 0.11397910306634174 1106 10-11 0.09702168472252737 1106 12-13 0.1472047911942056 1106 14-15 0.14979832221779077 1106 16-17 0.08207636759726 1106 18-19 0.09235772040248236 1106 20-21 0.1933383196356644 1106 22-23 0.17565937554685007 1106 24-25 0.17605640162595648 1106 26-27 0.14631170811041017 1106 28-29 0.2469330228379576 1106 30-31 0.16344428114047105 1106 32-33 0.17756298726148145 1106 34-35 0.21015500686519317 1106 36-37 0.07536664310558194 1106 38-39 0.0027598060312925554 1106 40-41 0.11077469830587461 1106 42-43 0.07313196536982502 1106 44-45 0.10893697315461992 1106 46-47 0.14032657168142038 1106 48-49 0.21491362646946754 1106 50-51 0.2089940415474274 1106 52-53 0.344291470632335 1106 54-55 0.31179799620534254 1106 56-57 0.38883298703767366 1106 58-59 0.40002260823935387 1106 60-61 0.3173945629125754 1106 62-63 0.43065779385546676 1106 64-65 0.4036960141054422 1106 66-67 0.48225740500491554 1106 68-69 0.5440694239787476 1106 70-71 0.5009941086540763 1106 72-73 0.3131918900983166 1106 74-75 0.37513855051038547 1106 76-77 0.3286451613519006 1106 78-79 0.38917274390648515 1106 80-81 0.4490042243736774 1106 82-83 0.5907387484883273 1106 84-85 0.5109911316332507 1106 86-87 0.30083894083597684 1106 88-89 0.3653134854091782 1106 90-91 0.351971185711367 1106 92-93 0.46946001887801714 1106 94-95 0.4033637017761862 1106 96-97 0.4047023665643117 1106 98-99 0.324020520981815 1106 100-101 0.28654482057397246 1107 1 -0.04475758103814442 1107 2 0.036228454814356326 1107 3 0.0474424289094415 1107 4 0.15724906575805875 1107 5 0.06801513458817965 1107 6 0.030601727258584788 1107 7 0.045088567101792876 1107 8 -0.011089161191762287 1107 9 -0.015906430839720542 1107 10-11 -0.00898854026255691 1107 12-13 0.011937445014204684 1107 14-15 0.09569015483130272 1107 16-17 0.031857454186777545 1107 18-19 0.03589076622537135 1107 20-21 0.0891809956698566 1107 22-23 -0.055073172044949104 1107 24-25 -0.009469120740803305 1107 26-27 0.045641585208549884 1107 28-29 0.05804829397501976 1107 30-31 -0.025385979733073327 1107 32-33 -0.07395633637731436 1107 34-35 0.06826964375741085 1107 36-37 0.06362946833832694 1107 38-39 -0.036697357446627166 1107 40-41 0.061601559876990564 1107 42-43 0.1526520534350837 1107 44-45 0.19081277572429656 1107 46-47 -0.03701307841122059 1107 48-49 0.07806525056327018 1107 50-51 -0.034596856986929936 1107 52-53 0.04680256991717613 1107 54-55 0.19081954074255947 1107 56-57 0.13918984089161768 1107 58-59 0.28727949406590625 1107 60-61 0.10659568518227047 1107 62-63 0.11823565548177584 1107 64-65 0.126514039989722 1107 66-67 0.15578164013583518 1107 68-69 0.1663343862729505 1107 70-71 0.10377093465957188 1107 72-73 0.10097210603317208 1107 74-75 0.1506366514209283 1107 76-77 0.09059838553401889 1107 78-79 0.16737039501349926 1107 80-81 0.14356263270029856 1107 82-83 0.21184821873245596 1107 84-85 0.2767290999876586 1107 86-87 0.33173431959679434 1107 88-89 0.344914442296151 1107 90-91 0.2845952201543014 1107 92-93 0.3404195719697256 1107 94-95 0.2935755020630033 1107 96-97 0.3408064681184726 1107 98-99 0.2357884706838007 1107 100-101 0.3698018501210356 1108 1 -0.26536953254714746 1108 2 -0.25138962218771255 1108 3 -0.2939104826040335 1108 4 -0.25478266377534453 1108 5 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10665.0 13 11788.0 14 12369.0 15 14176.0 16 17295.0 17 23076.0 18 19769.0 19 14523.0 20 14051.0 21 14140.0 22 15487.0 23 17557.0 24 20551.0 25 23922.0 26 26871.0 27 31674.0 28 38175.0 29 46492.0 30 57534.0 31 73264.0 32 93776.0 33 125537.0 34 176056.0 35 261207.0 36 412114.0 37 675310.0 38 592265.0 39 278.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.678335064742264 23.453939474026704 12.730565586558187 23.137159874672843 2 14.805845925653715 20.91100761197945 42.74947973045843 21.5336667319084 3 17.50633173041557 30.6538300103884 29.164704210247066 22.675134048948962 4 11.602968162453404 17.81869417761243 42.49236839538752 28.08596926454665 5 12.437218253964291 38.5177100361254 35.77124060957501 13.27383110033529 6 26.23225178967466 40.61205924674394 20.21907142288913 12.936617540692277 7 24.585654576397896 32.410894056785615 26.615433724204202 16.388017642612283 8 24.460534624009384 35.93881737513052 21.321385193373402 18.279262807486692 9 26.404437347989578 14.84230858461341 23.87291354407674 34.88034052332027 10-11 24.10418476215805 26.897482339161005 29.131750932366813 19.866581966314133 12-13 24.445759590082865 25.004872159689008 29.133784096172356 21.415584154055775 14-15 22.188809829412612 25.997201259033996 26.91336618503113 24.900622726522265 16-17 20.296418423019638 28.122701628788427 28.701314890940594 22.879565057251337 18-19 19.78348040756825 28.9665980240719 30.59577922104941 20.654142347310447 20-21 21.071448364378387 27.638948825797815 29.504531230314786 21.785071579509015 22-23 21.678055278454302 28.202050582545052 28.653800514393126 21.466093624607524 24-25 21.196026183002417 28.156879661939634 29.7132907645685 20.933803390489448 26-27 21.193252909063855 27.94658486438016 28.94632956508917 21.913832661466817 28-29 20.527277913748783 28.993029031224072 28.82189196413028 21.657801090896868 30-31 21.881096435562558 28.153853271808533 28.619183661377946 21.345866631250964 32-33 22.048339106692136 28.535687146196626 27.921504288661946 21.49446945844929 34-35 22.05230007352753 27.641374062723738 29.361066092559472 20.94525977118926 36-37 21.973829659120526 27.84185224129145 29.15714287140804 21.02717522817998 38-39 22.503783721890866 27.375606502243272 29.797538009471037 20.323071766394822 40-41 21.52139759212598 27.092926495601322 29.972539451654455 21.413136460618247 42-43 21.9876764003266 27.26428336290744 28.930152843391692 21.81788739337427 44-45 21.39650075125603 28.873778335200655 28.8592824556253 20.870438457918013 46-47 22.179152225804934 27.735757247026022 28.133729154161774 21.95136137300727 48-49 22.57138283292487 27.026289196720754 28.69649016248185 21.705837807872523 50-51 22.31798127304718 27.383695023638836 27.616566451310444 22.681757252003543 52-53 21.034487076842673 28.21303193909557 28.107389229185575 22.645091754876187 54-55 21.576189879125906 28.556297635526523 27.557920609667956 22.30959187567961 56-57 22.506477867598644 27.50583112397366 27.7662309700904 22.22146003833729 58-59 21.86299402708715 27.323184705657923 29.019256956756927 21.794564310498004 60-61 21.882148619363765 29.13178414354565 27.4074635236339 21.57860371345668 62-63 22.260921449856163 28.024425564249256 26.74793504469794 22.96671794119664 64-65 20.918494101613785 28.162139172982897 27.480875273362333 23.43849145204098 66-67 22.015080563750224 26.59118432491563 28.09533684357865 23.2983982677555 68-69 20.926374515893954 27.580604834196755 27.68607027237371 23.80695037753558 70-71 21.3858773169271 27.19936875153266 28.319300955714183 23.095452975826053 72-73 22.473465953523107 26.998369225907332 28.645363834389038 21.882800986180523 74-75 22.329492083434054 28.010800221826116 27.511239775463793 22.148467919276037 76-77 21.737875327354438 27.811179279719767 28.263514463875474 22.18743092905032 78-79 21.274179378563858 27.850021850508455 28.11506676470033 22.760732006227354 80-81 21.138841309386425 28.092123338088133 28.960288372400456 21.80874698012499 82-83 22.41708561582397 26.711002265454532 29.589406183898166 21.28250593482333 84-85 23.19670091780045 27.29939457976934 27.663838168357252 21.840066334072954 86-87 21.44056870823474 28.396524249376398 28.253736602084413 21.909170440304454 88-89 22.455809847083078 27.067591652306568 28.239722831299563 22.236875669310795 90-91 22.162199500089347 26.500203304562582 29.150553994505017 22.18704320084305 92-93 22.35176763414613 27.43216892008638 28.216268251565563 21.999795194201926 94-95 20.712149202368884 27.383723256682497 29.09426185776023 22.80986568318839 96-97 21.4182732729805 27.437374393392687 28.025653704323112 23.118698629303704 98-99 20.55746672400172 28.33643342633612 28.25154754642639 22.854552303235764 100-101 21.322406389768688 27.13463393561299 27.899572206559498 23.643387468058823 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 70.0 1 45.5 2 38.5 3 54.0 4 45.0 5 47.5 6 81.5 7 134.5 8 215.0 9 274.5 10 274.5 11 308.0 12 399.0 13 555.0 14 766.0 15 1019.0 16 1327.5 17 1656.0 18 2007.5 19 2348.5 20 2669.5 21 2966.5 22 3265.5 23 3610.0 24 3860.0 25 4138.0 26 4837.0 27 5873.0 28 8868.5 29 18838.5 30 34180.0 31 48639.0 32 67035.0 33 89367.5 34 115986.5 35 135528.5 36 146256.0 37 167664.5 38 165878.0 39 131601.0 40 101428.5 41 80121.5 42 70629.0 43 70291.0 44 72421.5 45 83564.5 46 95213.0 47 102595.0 48 107598.0 49 105263.5 50 100944.0 51 101624.5 52 100135.5 53 95970.5 54 91686.5 55 81592.5 56 72470.5 57 59243.0 58 45609.0 59 42475.5 60 36641.5 61 27218.0 62 20469.5 63 16935.5 64 13226.5 65 8059.5 66 5435.0 67 4112.5 68 3324.5 69 2711.0 70 2194.5 71 1836.5 72 1537.0 73 1258.5 74 1020.0 75 865.0 76 731.5 77 591.5 78 481.5 79 392.0 80 295.0 81 261.5 82 244.0 83 189.5 84 149.0 85 125.0 86 96.5 87 72.0 88 58.0 89 50.0 90 40.5 91 34.0 92 29.5 93 19.0 94 11.5 95 10.0 96 7.0 97 6.0 98 5.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.657087118303428E-4 2 0.0034949707371092994 3 1.3314174236606855E-4 4 0.001131704810111583 5 0.0019971261354910282 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.664271779575857E-5 12-13 8.321358897879285E-5 14-15 0.003628112479475368 16-17 0.005042743492114847 18-19 0.0036114697616796095 20-21 1.664271779575857E-5 22-23 0.0 24-25 4.992815338727571E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.3299804914062E-4 42-43 0.0 44-45 4.992815338727571E-4 46-47 0.7095622732221666 48-49 0.5084516713782201 50-51 0.3887239595555329 52-53 0.32584777172315704 54-55 0.3164279934507577 56-57 0.2842409772337606 58-59 0.2843408335405352 60-61 0.262638729534866 62-63 0.25638106764366075 64-65 0.25321895126246663 66-67 0.24233461382404056 68-69 0.2214313602725678 70-71 0.23572745485912439 72-73 0.7830065868548493 74-75 0.5155747545948047 76-77 0.6076921975943285 78-79 0.3364325402412595 80-81 0.2911144196834089 82-83 0.27392249220039033 84-85 0.2652349935110043 86-87 0.23995470517924705 88-89 0.2429004662290963 90-91 0.25235352993708715 92-93 0.21152894318409143 94-95 0.18097291331107868 96-97 0.1373523499683955 98-99 0.14668891465181602 100-101 0.1421620954113697 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3004317.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.11317551648129 #Duplication Level Percentage of deduplicated Percentage of total 1 81.73342931985098 18.89119097433634 2 8.97647542655494 4.149497041066912 3 2.9586952838600697 2.0515453018692966 4 1.3738376032980912 1.2701499862468313 5 0.7942895315734999 0.9179276677081006 6 0.5527186098900098 0.7665049344968008 7 0.3950044121627941 0.6390864415672224 8 0.28264149685174716 0.5226194019980341 9 0.22808648305184526 0.4744622624142812 >10 1.838628136961974 8.83885810516844 >50 0.335821394422901 5.470139983917363 >100 0.41062970942843685 20.220098973548076 >500 0.07616034273369217 12.163422726508392 >1k 0.040686418836820344 17.743329358879343 >5k 0.002027081365535533 3.2239728269944243 >10k+ 8.687491566580856E-4 2.657194013280158 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 16919 0.5631562847728785 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 15501 0.5159575371041072 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 13010 0.433043517045638 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 11946 0.3976278135762638 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 11831 0.39379998848323927 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 10193 0.3392784449843342 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 9635 0.3207051719242677 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 8096 0.26947888654892277 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 8033 0.2673819041066572 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 7593 0.25273631244638967 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 7458 0.24824277864153482 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 7151 0.23802414991493906 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 6769 0.2253091135189795 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 6738 0.2242772650156425 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 6500 0.21635533134486143 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 5902 0.19645064086113417 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 5847 0.1946199419036007 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 5763 0.19182396531391327 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 5761 0.19175739444273027 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 5090 0.16942286716082225 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 4872 0.1621666422018715 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 4869 0.16206678589509696 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 4598 0.1530464328497958 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 4541 0.15114916302107934 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 4413 0.14688862726536514 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 4376 0.145657066148479 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 4307 0.1433603710926643 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 4269 0.14209552454018667 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4225 0.14063096537415992 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 4142 0.13786827422006398 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 4138 0.13773513247769795 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 4086 0.13600428982693905 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4077 0.1357047209066154 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 4074 0.13560486459984084 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 4062 0.13520543937274263 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 3992 0.13287545888133642 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 3978 0.13240946278305518 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 3920 0.1304789075187472 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 3809 0.1267842241680888 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 3805 0.1266510824257227 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 3753 0.12492023977496382 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 3686 0.12269011559033217 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 3573 0.11892886136849075 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 3526 0.11736444589568944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3509 0.11679859349063365 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 3463 0.11526746345342385 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 3369 0.11213863250782125 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 3338 0.11110678400448422 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 3206 0.10671310650640395 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 3197 0.10641353758608028 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 3177 0.10574782887424995 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 3164 0.10531511821156023 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 3150 0.104849122113279 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 3108 0.10345113381843528 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 3077 0.10241928531509822 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 3072 0.10225285813714065 No Hit GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC 3053 0.10162043486090183 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 3036 0.10105458245584605 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 3005 0.10002273395250902 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.328543559151714E-5 0.0 0.0 0.0 6 0.0 3.328543559151714E-5 0.0 0.0 0.0 7 0.0 3.328543559151714E-5 0.0 0.0 0.0 8 0.0 3.328543559151714E-5 0.0 0.0 0.0 9 0.0 3.328543559151714E-5 0.0 0.0 0.0 10-11 0.0 3.328543559151714E-5 0.0 0.0 0.0 12-13 0.0 3.328543559151714E-5 0.0 0.0 0.0 14-15 0.0 3.328543559151714E-5 0.0 0.0 0.0 16-17 0.0 3.328543559151714E-5 0.0 0.0 0.0 18-19 0.0 3.328543559151714E-5 0.0 0.0 0.0 20-21 0.0 6.657087118303428E-5 0.0 0.0 0.0 22-23 0.0 6.657087118303428E-5 0.0 8.321358897879285E-5 0.0 24-25 0.0 6.657087118303428E-5 0.0 3.1621163811941283E-4 0.0 26-27 0.0 8.321358897879285E-5 0.0 7.988504541964114E-4 0.0 28-29 0.0 9.985630677455141E-5 0.0 0.0018140562397376842 0.0 30-31 0.0 9.985630677455141E-5 0.0 0.006107877431043395 0.0 32-33 0.0 9.985630677455141E-5 0.0 0.01910584002953084 0.0 34-35 0.0 9.985630677455141E-5 0.0 0.04047508967928484 0.0 36-37 0.0 9.985630677455141E-5 0.0 0.07376052527080199 0.0 38-39 0.0 9.985630677455141E-5 0.0 0.12179140882936121 0.0 40-41 0.0 9.985630677455141E-5 0.0 0.17386647281228979 0.0 42-43 0.0 9.985630677455141E-5 0.0 0.22973607645265132 0.0 44-45 0.0 1.1649902457030998E-4 0.0 0.29560795348826374 0.0 46-47 0.0 1.3314174236606855E-4 0.0 0.37622527849091825 0.0 48-49 0.0 1.3314174236606855E-4 0.0 0.4633665488695101 0.0 50-51 0.0 1.3314174236606855E-4 0.0 0.5538530055250495 0.0 52-53 0.0 1.3314174236606855E-4 0.0 0.6472352950770508 0.0 54-55 0.0 2.163553313448614E-4 0.0 0.7519179900123723 0.0 56-57 0.0 2.3299804914061998E-4 0.0 0.8784858588491161 0.0 58-59 0.0 2.662834847321371E-4 0.0 1.029601736434604 0.0 60-61 0.0 2.9956892032365426E-4 0.0 1.1873081302672122 0.0 62-63 0.0 2.9956892032365426E-4 0.0 1.3497410559538157 0.0 64-65 0.0 3.6613979150668856E-4 0.0 1.5263535772024057 0.0 66-67 0.0 3.6613979150668856E-4 0.0 1.7219887248915478 0.0 68-69 3.328543559151714E-5 3.6613979150668856E-4 0.0 1.9428542327590597 0.0 70-71 3.328543559151714E-5 3.8278250930244713E-4 0.0 2.1783320468512475 0.0 72-73 3.328543559151714E-5 3.9942522709820566E-4 0.0 2.4367934542193783 0.0 74-75 3.328543559151714E-5 3.9942522709820566E-4 0.0 2.690361902555556 0.0 76-77 3.328543559151714E-5 3.9942522709820566E-4 0.0 2.98412251436849 0.0 78-79 3.328543559151714E-5 3.9942522709820566E-4 0.0 3.296905752621977 0.0 80-81 3.328543559151714E-5 3.9942522709820566E-4 0.0 3.654241546414709 0.0 82-83 3.328543559151714E-5 3.9942522709820566E-4 0.0 4.036591345054466 0.0 84-85 3.328543559151714E-5 3.9942522709820566E-4 0.0 4.40953800813962 0.0 86-87 3.328543559151714E-5 3.9942522709820566E-4 0.0 4.82495688703955 0.0 88-89 3.328543559151714E-5 3.9942522709820566E-4 0.0 5.2808175701831725 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2250 0.0 53.686657 2 ACCTGGG 2325 0.0 52.565186 3 GTACCTG 2595 0.0 46.91421 1 CCTGGGG 2510 0.0 44.160744 4 CTGGGGG 2430 0.0 41.91084 5 GTACCCG 250 0.0 37.89646 1 GTACTAG 2505 0.0 34.606052 1 GTATCAA 9900 0.0 31.963179 1 TATTCTC 2285 0.0 30.681017 5 GTACACG 205 1.8189894E-12 30.039877 1 TCTCTGC 2385 0.0 29.990437 8 TATTCGC 530 0.0 29.497833 9 TAGGCAT 3365 0.0 28.998558 5 TTCTCTG 2520 0.0 28.570827 7 TGGGGGG 9195 0.0 28.487423 6 CTGTGCG 2970 0.0 27.914745 9 TAGTACT 3010 0.0 27.85486 4 GGTATCA 6565 0.0 27.852312 1 CAACGCA 10920 0.0 27.80543 5 GCATCGT 2825 0.0 27.49917 8 >>END_MODULE