FastQCFastQC Report
Sat 18 Jun 2016
SRR1931077_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931077_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683936
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA35570.21123130570282955No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA33320.19786975276970148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.1882494346578492No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC29590.17571926724056022No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC27210.16158571347129583No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC27140.16117002071337627No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT25150.14935246945252076No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC24810.14733339034262585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23560.13991030537977692No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22870.1358127624802843No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA19340.11484997054519885No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT18650.11075242764570625No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC17820.10582349923037455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG27350.058.0039522
ACCTGGG28000.056.3191833
GTACCTG28500.055.3311231
CCTGGGG27250.046.225894
CTGGGGG24600.041.1954045
TATCACG1601.8189894E-1235.51662
GTACCCG3200.035.51661
GTACACG2450.030.9260221
TGGGGGG48450.028.1493826
ATAACGC2500.026.5190643
GTACTAG11650.026.4215071
AGGGCTA15500.026.2746495
TAGTACT13800.025.7366684
CTGTGCG13550.025.1652959
GCTATTG15900.024.720158
GTATAAG10550.024.6876851
TAACGCA2701.8189894E-1224.554694
GGGCTAT17250.023.6091026
CTATTGA16800.023.397949
GCATCGT12550.023.3947338
Produced by FastQC (version 0.11.5)