##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931077_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1683936
Sequences flagged as poor quality	0
Sequence length	101
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.363314282728084	33.0	33.0	33.0	27.0	33.0
2	30.24631814985843	33.0	33.0	33.0	27.0	33.0
3	30.286817907568935	33.0	33.0	33.0	27.0	33.0
4	33.78824551526899	37.0	37.0	37.0	27.0	37.0
5	33.73140784447865	37.0	37.0	37.0	27.0	37.0
6	33.83647656443	37.0	37.0	37.0	27.0	37.0
7	33.80015451893659	37.0	37.0	37.0	27.0	37.0
8	33.7921001748285	37.0	37.0	37.0	27.0	37.0
9	33.82598032229254	37.0	37.0	37.0	27.0	37.0
10-11	33.689640817703285	37.0	37.0	37.0	27.0	37.0
12-13	33.56113860621781	37.0	37.0	37.0	27.0	37.0
14-15	34.9827561736313	40.0	37.0	40.0	27.0	40.0
16-17	34.9042520024514	37.0	37.0	40.0	27.0	40.0
18-19	34.83423004199685	37.0	37.0	40.0	27.0	40.0
20-21	34.72409343347966	37.0	37.0	40.0	24.5	40.0
22-23	34.59204565969253	37.0	37.0	40.0	22.0	40.0
24-25	34.43409725785303	37.0	37.0	40.0	22.0	40.0
26-27	34.230133746175625	37.0	33.0	40.0	22.0	40.0
28-29	34.071530331319	37.0	33.0	40.0	15.0	40.0
30-31	33.8885875116394	37.0	33.0	40.0	15.0	40.0
32-33	33.68942554823936	37.0	33.0	40.0	15.0	40.0
34-35	33.510232277236184	37.0	33.0	40.0	15.0	40.0
36-37	33.240261803298935	37.0	33.0	40.0	15.0	40.0
38-39	33.00026930952245	37.0	33.0	40.0	6.0	40.0
40-41	32.74548468587879	37.0	33.0	40.0	6.0	40.0
42-43	32.489504648632725	37.0	33.0	40.0	6.0	40.0
44-45	32.148319176025694	37.0	33.0	40.0	4.0	40.0
46-47	31.922429652908423	37.0	33.0	40.0	2.0	40.0
48-49	31.826701846150925	37.0	33.0	40.0	2.0	40.0
50-51	31.02027422657393	37.0	30.0	38.5	2.0	40.0
52-53	31.151123914448057	37.0	33.0	38.5	2.0	40.0
54-55	31.560820304334605	37.0	33.0	40.0	2.0	40.0
56-57	31.457131684339547	37.0	33.0	40.0	2.0	40.0
58-59	31.249029951257054	37.0	33.0	40.0	2.0	40.0
60-61	31.22062180510423	37.0	33.0	40.0	2.0	40.0
62-63	31.00689960901127	37.0	33.0	40.0	2.0	40.0
64-65	30.746640014822418	37.0	33.0	37.0	2.0	40.0
66-67	30.451910880223476	37.0	30.0	37.0	2.0	40.0
68-69	30.166081727571594	37.0	27.0	37.0	2.0	40.0
70-71	29.90888549208521	37.0	27.0	37.0	2.0	40.0
72-73	29.316267661003742	37.0	27.0	37.0	2.0	40.0
74-75	29.133959960473558	35.0	27.0	37.0	2.0	38.5
76-77	28.800215388233283	33.0	27.0	37.0	2.0	37.0
78-79	28.539782390779695	33.0	27.0	37.0	2.0	37.0
80-81	28.296658245919083	33.0	27.0	37.0	2.0	37.0
82-83	28.02736327271345	33.0	27.0	37.0	2.0	37.0
84-85	27.625506848241265	33.0	27.0	37.0	2.0	37.0
86-87	27.443009413659425	33.0	27.0	37.0	2.0	37.0
88-89	27.245044348478803	33.0	27.0	37.0	2.0	37.0
90-91	26.93473148623226	33.0	22.0	37.0	2.0	37.0
92-93	26.621696727191534	33.0	22.0	37.0	2.0	37.0
94-95	26.333531084316743	33.0	22.0	37.0	2.0	37.0
96-97	25.999049548201356	33.0	15.0	37.0	2.0	37.0
98-99	25.63520199104954	33.0	15.0	37.0	2.0	37.0
100-101	24.91355669099063	33.0	2.0	37.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.25231441776161745
1101	2	-0.24824720353824148
1101	3	-0.2511217427401071
1101	4	-0.4301683782096646
1101	5	-0.26874830778618275
1101	6	-0.2560275237645513
1101	7	-0.22955493426982088
1101	8	-0.2910177344404232
1101	9	-0.2705428424849785
1101	10-11	-0.2623192354628969
1101	12-13	-0.10706316082982426
1101	14-15	-0.08210624272050637
1101	16-17	-0.08251902944589773
1101	18-19	-0.11626262316345759
1101	20-21	-0.11701952095878454
1101	22-23	-0.06365785694546844
1101	24-25	-0.11534038580103356
1101	26-27	-0.06126999003775779
1101	28-29	-0.017098140454862687
1101	30-31	-0.15105397545757882
1101	32-33	-0.2093348021702397
1101	34-35	-0.2840254388312289
1101	36-37	-0.14365515887705982
1101	38-39	-0.19911100397855108
1101	40-41	-0.26216989286304937
1101	42-43	-0.25389416587615443
1101	44-45	-0.2891845110601956
1101	46-47	-0.23020972231156023
1101	48-49	-0.11954721577270888
1101	50-51	-0.12379684773461719
1101	52-53	0.0071714595898306754
1101	54-55	0.066838593917808
1101	56-57	0.04399944506387854
1101	58-59	-0.06942738188552866
1101	60-61	-0.08742669853858587
1101	62-63	0.021391204293038868
1101	64-65	0.049336992845539385
1101	66-67	0.08521198015202813
1101	68-69	0.03248321699238588
1101	70-71	-0.027831681715170475
1101	72-73	0.02550596463537147
1101	74-75	0.030047083056231827
1101	76-77	-0.16110762131809153
1101	78-79	-0.34619641462195716
1101	80-81	-0.2318805234527055
1101	82-83	-0.25793780732027116
1101	84-85	-0.24844357510331605
1101	86-87	-0.30698142297060826
1101	88-89	-0.3599371963865359
1101	90-91	-0.26347906287790934
1101	92-93	-0.2266823352970988
1101	94-95	-0.4533591466772826
1101	96-97	-0.7146238658912942
1101	98-99	-0.4639971342566902
1101	100-101	-0.4073725175995726
1102	1	0.279840872552942
1102	2	0.22578646272780034
1102	3	0.18270615453426586
1102	4	0.17046433208361833
1102	5	0.18126688831256388
1102	6	0.20006783866239886
1102	7	0.19072598633746907
1102	8	0.07479035264703526
1102	9	0.21653356626244857
1102	10-11	0.16674520290310113
1102	12-13	0.3128352670646919
1102	14-15	0.40910246878846834
1102	16-17	0.37508730870874984
1102	18-19	0.4194956967006078
1102	20-21	0.33862686582337176
1102	22-23	0.44796284152249655
1102	24-25	0.4144715970135593
1102	26-27	0.28331450027127403
1102	28-29	0.474113161298213
1102	30-31	0.3679601430996158
1102	32-33	0.2689175083275117
1102	34-35	0.28778735213952444
1102	36-37	0.36344988070186446
1102	38-39	0.18982709954833155
1102	40-41	0.4826107652661804
1102	42-43	0.36205638463705014
1102	44-45	0.16298404130418476
1102	46-47	-0.025523452677511216
1102	48-49	0.25746281365246304
1102	50-51	0.13544859688941813
1102	52-53	0.41748771112670724
1102	54-55	0.41445021617042244
1102	56-57	0.5199827017259899
1102	58-59	0.4518575173367054
1102	60-61	0.5407532578071645
1102	62-63	0.3706977096047659
1102	64-65	0.42720871014284256
1102	66-67	0.33404378006085267
1102	68-69	0.2614720202305385
1102	70-71	0.22906182126188668
1102	72-73	0.07739287112410409
1102	74-75	0.21453031208907092
1102	76-77	0.17956819063709517
1102	78-79	0.281414551435482
1102	80-81	0.2806942482604491
1102	82-83	0.20402704824772044
1102	84-85	0.347291847002861
1102	86-87	0.5088729431448513
1102	88-89	0.40761429766626023
1102	90-91	0.5263226280334443
1102	92-93	0.3692957063402105
1102	94-95	0.49442498583269767
1102	96-97	0.5410366808919669
1102	98-99	0.6415112788561608
1102	100-101	0.5480174350162272
1103	1	0.12627734152274073
1103	2	0.030884700119276687
1103	3	0.07288456229086648
1103	4	0.11441361229262981
1103	5	-0.056249688266696296
1103	6	-0.09998930202424816
1103	7	-0.15798454984364696
1103	8	-0.18001706176992371
1103	9	-0.03632800959842086
1103	10-11	0.07186936829401702
1103	12-13	0.2107617289827317
1103	14-15	0.32843109049235153
1103	16-17	0.22191331696937056
1103	18-19	0.2189663609032877
1103	20-21	0.20007308669359958
1103	22-23	0.07351484367600847
1103	24-25	0.21876350665201016
1103	26-27	0.1706350479631098
1103	28-29	0.16583874397633025
1103	30-31	0.29421624732687945
1103	32-33	0.24696169492322184
1103	34-35	0.290031692982069
1103	36-37	0.2859798671763727
1103	38-39	0.3916976313035221
1103	40-41	0.3654636874508057
1103	42-43	0.20498947718679972
1103	44-45	0.14134468986748772
1103	46-47	0.1240115360842644
1103	48-49	0.2732572178286894
1103	50-51	0.20417018137093024
1103	52-53	0.4717891166885124
1103	54-55	0.5039457844642783
1103	56-57	0.566619245672296
1103	58-59	0.5848099402834812
1103	60-61	0.5758923028971132
1103	62-63	0.5892593909942399
1103	64-65	0.5962871349095309
1103	66-67	0.5387544186327986
1103	68-69	0.6430603331435059
1103	70-71	0.6874655482879461
1103	72-73	0.3581233305781666
1103	74-75	0.3743972783314753
1103	76-77	0.3544729836837668
1103	78-79	0.6055746163710864
1103	80-81	0.6639371226021318
1103	82-83	0.6082032727676783
1103	84-85	0.6568378923223612
1103	86-87	0.6601543560721908
1103	88-89	0.6117842879061044
1103	90-91	0.5723165456880999
1103	92-93	0.3176455212935636
1103	94-95	0.4770445560224381
1103	96-97	0.6191637710272708
1103	98-99	0.7050282716045793
1103	100-101	0.8702163853451168
1104	1	0.09947129652409714
1104	2	0.21749353720250042
1104	3	0.053309474191109985
1104	4	0.20567818263689475
1104	5	0.2601538086159323
1104	6	0.020758190521156905
1104	7	0.36277310805822083
1104	8	0.06381030788761421
1104	9	0.06093863899650387
1104	10-11	-0.028423732817401515
1104	12-13	0.04227546350880118
1104	14-15	0.1941834398191773
1104	16-17	0.1975074255805609
1104	18-19	0.1692532498524173
1104	20-21	0.1471008494115864
1104	22-23	0.2330352647476559
1104	24-25	0.23998368827304262
1104	26-27	0.28598530096753905
1104	28-29	0.19897328811656934
1104	30-31	0.03132036316676334
1104	32-33	0.12657789624245908
1104	34-35	0.07725101619521979
1104	36-37	0.11070992048801997
1104	38-39	-0.12222608334362661
1104	40-41	-0.07536433730749081
1104	42-43	0.09517990819791322
1104	44-45	-0.033132923758607546
1104	46-47	0.04667519832335287
1104	48-49	-0.047126580852076216
1104	50-51	-0.06470293239070202
1104	52-53	0.2353129939813634
1104	54-55	0.3516269537061696
1104	56-57	0.3980669053813415
1104	58-59	0.17498399377584306
1104	60-61	0.24871748135559457
1104	62-63	0.300888558790394
1104	64-65	0.17823910924765585
1104	66-67	0.23084690078694692
1104	68-69	0.3855255450347137
1104	70-71	0.28324636061287123
1104	72-73	0.33575728738669497
1104	74-75	0.11205766506681414
1104	76-77	0.30013576492529737
1104	78-79	0.33029168061613845
1104	80-81	0.28223984691766546
1104	82-83	0.05059393871147577
1104	84-85	0.1756635677538263
1104	86-87	0.20264159290240613
1104	88-89	0.010102486153144241
1104	90-91	0.20021141331256942
1104	92-93	0.09100946364469564
1104	94-95	-0.03851126307939978
1104	96-97	-0.1667402680076968
1104	98-99	-0.22371935647891306
1104	100-101	-0.12956167103872573
1105	1	-0.10834031980889947
1105	2	-0.11624698988719828
1105	3	-0.060997448102749985
1105	4	-0.05685716404334329
1105	5	-0.2594607711827308
1105	6	-0.2081963960640678
1105	7	-0.19530007370854463
1105	8	-0.1740375034459447
1105	9	-0.22984860226579684
1105	10-11	-0.12062696209010682
1105	12-13	-0.11540890477532173
1105	14-15	-0.12909376298593855
1105	16-17	-0.21704692347497456
1105	18-19	-0.2224841101747259
1105	20-21	-0.14188159035767
1105	22-23	-0.24584014527383147
1105	24-25	-0.317847015267283
1105	26-27	-0.030112284147264745
1105	28-29	-0.23051432640667713
1105	30-31	-0.3382185747361959
1105	32-33	-0.1284144688746025
1105	34-35	0.001513684445420438
1105	36-37	-0.08830461406689238
1105	38-39	-0.19683232132575057
1105	40-41	-0.30656767968957155
1105	42-43	-0.1503480298181472
1105	44-45	-0.19915416004409536
1105	46-47	-0.17843861798701255
1105	48-49	0.02842341349245814
1105	50-51	-0.05936063513926371
1105	52-53	0.02706378574699997
1105	54-55	0.015622825213917224
1105	56-57	0.11298673911055701
1105	58-59	0.11153034034297704
1105	60-61	0.1480819480448794
1105	62-63	0.07648803848725905
1105	64-65	-0.005451932489705769
1105	66-67	-0.03568544849480659
1105	68-69	0.06494953553352545
1105	70-71	0.2069802425266829
1105	72-73	0.13988860737312336
1105	74-75	0.08877834146088759
1105	76-77	0.10176171221632657
1105	78-79	0.12084634072245848
1105	80-81	0.24571753609309965
1105	82-83	0.4276793928456222
1105	84-85	0.2850085230756214
1105	86-87	0.2636506699298913
1105	88-89	0.10647874449496086
1105	90-91	0.2383589644945623
1105	92-93	0.2328959866624558
1105	94-95	0.2121073038247978
1105	96-97	0.14017354264720439
1105	98-99	0.20209220329939015
1105	100-101	0.08042659386343587
1106	1	0.09577481793266074
1106	2	0.021255041804337793
1106	3	0.0486426074444104
1106	4	0.0576489709522221
1106	5	0.08254776619896376
1106	6	0.12307411689170777
1106	7	0.07812925051520381
1106	8	0.16547597415411985
1106	9	0.2745640891765788
1106	10-11	0.20458697146430893
1106	12-13	0.06742052153201428
1106	14-15	0.08352667711409367
1106	16-17	0.14356584894991187
1106	18-19	-0.07285359288467674
1106	20-21	0.19557774016788443
1106	22-23	0.13819232757278854
1106	24-25	0.20013024689649228
1106	26-27	0.15668107927886865
1106	28-29	0.07976560504333463
1106	30-31	0.1914719237910063
1106	32-33	0.10432399468184173
1106	34-35	0.24525571807597402
1106	36-37	0.2597145223287498
1106	38-39	0.11860775018978842
1106	40-41	0.22563356185217742
1106	42-43	0.1345806685020321
1106	44-45	0.1994776204590778
1106	46-47	0.2728581715126346
1106	48-49	0.12031539593863982
1106	50-51	0.16249544696103868
1106	52-53	0.12422105720665755
1106	54-55	0.30715416459052136
1106	56-57	0.236909942596224
1106	58-59	0.23770658281145884
1106	60-61	0.2282467930802845
1106	62-63	0.2563907596707473
1106	64-65	0.35256383913961287
1106	66-67	0.31184429974653227
1106	68-69	0.19268340817997043
1106	70-71	0.17626106649522555
1106	72-73	0.004694862416705803
1106	74-75	0.35107827331011165
1106	76-77	0.20137626112377305
1106	78-79	0.30146264900348996
1106	80-81	0.4726735203870511
1106	82-83	0.3223276321888662
1106	84-85	0.5944935997666292
1106	86-87	0.5148639818579888
1106	88-89	0.4279946430158894
1106	90-91	0.3425633540888775
1106	92-93	0.27811680658187043
1106	94-95	0.34390420310707626
1106	96-97	0.38656105252051276
1106	98-99	0.32415979518174964
1106	100-101	0.4243573965883236
1107	1	-0.005432331593816286
1107	2	0.00808310636349674
1107	3	0.04574144698022309
1107	4	0.026369792614218568
1107	5	0.07100981768622461
1107	6	-0.17411142399131307
1107	7	-0.031614647043817
1107	8	0.05006314235473752
1107	9	-0.04302961497175062
1107	10-11	0.03383533747738454
1107	12-13	-0.07980503535740979
1107	14-15	0.06840396136112759
1107	16-17	0.14660039610210163
1107	18-19	-0.031086886202004393
1107	20-21	0.0384982417018378
1107	22-23	-0.05860305724780801
1107	24-25	0.04575182886261331
1107	26-27	0.1454099041421415
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2312	80-81	-0.45174672694510676
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2312	84-85	-1.5945564611523046
2312	86-87	-1.0003165312418218
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2312	90-91	-0.5511711365124761
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2312	98-99	-0.579257162655697
2312	100-101	-0.509645917197254
2313	1	-0.4022768510483168
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2313	24-25	-0.7866935359354699
2313	26-27	-2.0043733316041
2313	28-29	-0.882994689498851
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2313	32-33	-0.7793427473141961
2313	34-35	-0.6849338768044149
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2314	4	-0.4695972395563359
2314	5	-0.4654939771345923
2314	6	-0.6007310825092063
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2314	80-81	-1.241166962533665
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2314	84-85	-1.3525785319780006
2314	86-87	-1.3206166335236276
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2314	90-91	-1.360800944446364
2314	92-93	-1.2490920982890543
2314	94-95	-1.3229322422777763
2314	96-97	-1.2979209594623597
2314	98-99	-1.305987240801592
2314	100-101	-1.5227010310445266
2315	1	-0.11603369579541223
2315	2	-0.1269861094948901
2315	3	-0.22010642442640105
2315	4	-0.22140035038921724
2315	5	-0.08881412533184374
2315	6	-0.20833647153720847
2315	7	-0.13996786736420574
2315	8	-0.1492513280570833
2315	9	-0.15938106146432318
2315	10-11	-0.25147720823981956
2315	12-13	-0.32873068088443347
2315	14-15	-0.519682247584818
2315	16-17	-0.4858715106713589
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2315	20-21	-0.44318572892942854
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2315	24-25	-0.42856709897733936
2315	26-27	-0.3238601291399874
2315	28-29	-0.23048379713689116
2315	30-31	-0.2834837842986744
2315	32-33	-0.23302031804325196
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2315	82-83	-0.42778067726394653
2315	84-85	-0.27650137080224013
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2315	90-91	-0.3790344584232237
2315	92-93	-0.3985546684597594
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2315	96-97	-0.45653001455147546
2315	98-99	-0.43537930271547154
2315	100-101	-0.44678037303565077
2316	1	-0.5579148318402929
2316	2	-1.1145147443710002
2316	3	-0.7181656810883936
2316	4	-0.5463404509034788
2316	5	-0.4565697938685389
2316	6	-0.6963691386713862
2316	7	-0.915456705606374
2316	8	-0.9204081186134161
2316	9	-0.8488957295335169
2316	10-11	-0.7798238363370729
2316	12-13	-0.7479160478783555
2316	14-15	-1.0884899556259526
2316	16-17	-1.0369025123076412
2316	18-19	-1.0992043070833972
2316	20-21	-1.31928890214121
2316	22-23	-0.9658311948796694
2316	24-25	-0.9579743862611139
2316	26-27	-0.9267502062788395
2316	28-29	-0.9431385131256675
2316	30-31	-0.9850797403529086
2316	32-33	-1.0849179629707848
2316	34-35	-0.776426520036658
2316	36-37	-1.1011482575658107
2316	38-39	-1.1647856071531493
2316	40-41	-1.0513193062515853
2316	42-43	-0.7870095327958744
2316	44-45	-0.9711168782099548
2316	46-47	-0.9562414683433076
2316	48-49	-1.1157238893406891
2316	50-51	-1.1006682533016772
2316	52-53	-1.2876036726853037
2316	54-55	-1.0779951995029435
2316	56-57	-1.077712080125906
2316	58-59	-1.0681345362448589
2316	60-61	-1.236015955497411
2316	62-63	-1.32499135839387
2316	64-65	-1.4050372882678737
2316	66-67	-1.1340547555277531
2316	68-69	-0.9718295274290547
2316	70-71	-1.035296600049655
2316	72-73	-0.7393048244145461
2316	74-75	-0.8395520657820477
2316	76-77	-1.0023694110581438
2316	78-79	-0.9744572841871744
2316	80-81	-0.8798848942831654
2316	82-83	-0.9638936389282762
2316	84-85	-0.8711971982917213
2316	86-87	-0.8614500220044263
2316	88-89	-1.025565215710209
2316	90-91	-0.8812637419669365
2316	92-93	-0.6994020270385306
2316	94-95	-0.7480221326446177
2316	96-97	-0.8308810547571781
2316	98-99	-0.6641541390876107
2316	100-101	-0.6840673646205069
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76134.0
3	11003.0
4	8118.0
5	6978.0
6	7125.0
7	7740.0
8	7740.0
9	8079.0
10	7906.0
11	8427.0
12	8919.0
13	9597.0
14	10067.0
15	10993.0
16	13171.0
17	16333.0
18	14182.0
19	11153.0
20	10968.0
21	10962.0
22	11850.0
23	13340.0
24	15212.0
25	17182.0
26	19631.0
27	22688.0
28	27186.0
29	32135.0
30	38785.0
31	47858.0
32	60935.0
33	78840.0
34	106045.0
35	150209.0
36	222996.0
37	334557.0
38	218630.0
39	262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02432883116143	20.132452218859715	12.25628411666057	23.586934833318292
2	15.764406294502823	20.46693295610975	40.65670872424552	23.111952025141903
3	18.935336424117143	27.715921851904856	28.87202622910321	24.47671549487479
4	12.534666259678346	16.73494014539935	39.23787081763083	31.492522777291477
5	13.057467441846654	37.49624386101395	34.4282057829694	15.018082914169998
6	28.844979856716645	37.730293787887426	19.311303992550783	14.113422362845144
7	26.869132793645363	31.623173327252342	23.725426619538986	17.78226725956331
8	26.214773008000304	34.039357790319826	20.73623938201927	19.009629819660603
9	26.8847509644072	15.218690021473499	21.832005491895178	36.064553522224124
10-11	25.51914844719409	25.8011366824917	28.08896183969042	20.590753030623798
12-13	25.80827817233984	24.104084808524323	28.047320130325737	22.040316888810104
14-15	22.825039299095366	25.304994830394783	26.423215179695003	25.446750690814852
16-17	21.725188482312802	27.376035205140614	27.484180551060224	23.41459576148636
18-19	21.434166509786643	27.587971092900492	29.264043632640625	21.71381876467224
20-21	22.715768295232124	26.765239296505328	28.43154371662581	22.087448691636737
22-23	22.768709737187162	26.76096359956673	28.007923103965947	22.462403559280162
24-25	22.471133107196472	26.66185056914277	28.89486298766699	21.972153335993767
26-27	22.437580763164398	26.8018499515421	28.289347101077478	22.47122218421603
28-29	21.817723476426657	27.27645231172681	28.666172586131538	22.23965162571499
30-31	22.63355020618361	26.893539897003212	28.28667479049085	22.18623510632233
32-33	22.900335879748397	27.170183427398666	27.68629567869563	22.24318501415731
34-35	22.71036428937917	26.750007126161563	28.52299612336811	22.016632461091156
36-37	22.813931170780837	26.567844621173254	28.28608094369382	22.33214326435209
38-39	22.796382997928664	26.74187142504228	28.448468350341106	22.01327722668795
40-41	22.51056826175143	26.236784291066122	28.59940728063193	22.65324016655052
42-43	22.604362636109688	26.560451228550253	27.83591537920681	22.99927075613325
44-45	22.538935796466113	27.196592961923503	27.815266229038567	22.44920501257181
46-47	22.833299037154724	26.82465640014548	27.489274468329473	22.85277009437032
48-49	23.282557245106933	26.486277382328634	27.59510703692527	22.636058335639166
50-51	22.659672027924493	26.931764111015838	27.332516841632014	23.076047019427655
52-53	22.357277869280328	27.37650730921321	27.149680959338703	23.11653386216776
54-55	22.453990106103916	27.165164905716026	27.18199733958169	23.198847648598363
56-57	23.183056183054692	26.67197786570211	27.046849242856315	23.09811670838688
58-59	22.768299089736214	26.849773714688713	27.56082422890853	22.821102966666547
60-61	22.68663382477077	27.54333388417202	27.1037050601233	22.66632723093391
62-63	22.814666654360394	26.96984953498668	26.857128055590206	23.358355755062714
64-65	22.034840413021332	27.319195988636615	27.20505004974905	23.440913548593002
66-67	22.638018127060892	26.673517326742253	27.00471856081365	23.683745985383208
68-69	22.229490593642748	26.92243133174686	27.051595384379123	23.79648269023127
70-71	22.45158924438264	26.57882642612895	27.27132287807062	23.698261451417796
72-73	23.013641305388994	26.503261556734802	27.486488007433092	22.99660913044311
74-75	23.019292147617108	27.181187618732622	26.91730418595543	22.882216047694843
76-77	22.941507603993553	26.79289493168686	26.858241050987903	23.407356413331684
78-79	22.54042283368653	26.961398369022394	27.082941003997586	23.415237793293493
80-81	22.301419474740737	26.989528172076316	27.54468992548204	23.164362427700905
82-83	22.925994480435563	26.253545015895252	28.206385733462447	22.61407477020674
84-85	23.13262052147815	26.74405171310067	27.030337677495808	23.092990087925372
86-87	22.28500753106191	27.292895797488853	27.36674783147091	23.05534883997833
88-89	22.964505168627092	26.50892773685993	27.150429285096383	23.37613780941659
90-91	22.898662560628956	26.325888162869653	27.4231338546858	23.35231542181559
92-93	23.130086950000848	26.61826423170558	27.290527987877766	22.96112083041581
94-95	22.087907543903647	26.672723470322406	27.820809047365337	23.418559938408613
96-97	22.305544933305818	26.91306056981911	27.347350341752303	23.434044155122777
98-99	22.16145728785182	27.38888715416401	26.89627485179254	23.55338070619163
100-101	22.444704147114624	26.81603535128255	26.761051736340335	23.978208765262487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	134.0
1	88.0
2	91.5
3	127.0
4	91.0
5	92.5
6	136.0
7	227.0
8	362.0
9	431.0
10	409.0
11	415.0
12	509.0
13	618.5
14	700.0
15	839.5
16	1070.5
17	1353.5
18	1587.0
19	1817.5
20	2093.5
21	2432.5
22	2812.5
23	3249.0
24	3822.5
25	4612.0
26	5870.0
27	7289.5
28	9287.5
29	13106.0
30	17972.0
31	22472.5
32	27756.0
33	33126.5
34	37550.5
35	42014.5
36	45916.5
37	52028.0
38	54772.0
39	52009.0
40	49921.5
41	47860.5
42	47314.5
43	49505.5
44	53049.0
45	58498.5
46	64470.0
47	69376.5
48	74209.0
49	73164.5
50	68005.0
51	65808.0
52	63386.5
53	60622.5
54	58040.5
55	52660.5
56	47204.5
57	39957.5
58	32902.0
59	30361.0
60	26261.0
61	20630.5
62	16375.0
63	13444.5
64	10567.5
65	7622.0
66	5847.0
67	4646.0
68	3789.5
69	3062.0
70	2484.5
71	2108.5
72	1749.5
73	1415.0
74	1176.0
75	963.5
76	784.5
77	680.0
78	560.5
79	428.5
80	356.5
81	302.5
82	252.0
83	198.0
84	143.5
85	110.0
86	90.0
87	74.0
88	60.0
89	48.5
90	44.0
91	34.0
92	24.5
93	20.5
94	15.0
95	10.0
96	5.5
97	4.0
98	2.5
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.7507743762233243E-4
2	0.0032067727039507437
3	2.9692339851395777E-4
4	0.0013064629534614142
5	0.0015440016722725806
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.9692339851395777E-5
12-13	8.907701955418733E-5
14-15	0.0032661573836535356
16-17	0.005404005852954032
18-19	0.0035036961024647014
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.4846169925697889E-4
42-43	0.0
44-45	5.938467970279155E-4
46-47	0.725918324686924
48-49	0.5247527221937175
50-51	0.40630997852649986
52-53	0.3432434486821352
54-55	0.33424666970716227
56-57	0.3024758660661688
58-59	0.3017929422495867
60-61	0.27783122398951027
62-63	0.27171460198012276
64-65	0.26764675142048155
66-67	0.2549087376242327
68-69	0.23171902020029264
70-71	0.24609011268836822
72-73	0.8057313342074758
74-75	0.5317304220587956
76-77	0.6258551393877202
78-79	0.3517948425593372
80-81	0.30559356175056535
82-83	0.2861153868080497
84-85	0.27738583889173934
86-87	0.2509596564239971
88-89	0.2543445831670562
90-91	0.26473690211504475
92-93	0.22076254679512763
94-95	0.18893235847443132
96-97	0.14578938867035326
98-99	0.1540141668091899
100-101	0.15000570092925147
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1683936.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.543018715686884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.76512076834769	48.41517643368194
2	6.579106245674704	7.176886301806558
3	1.6907859417792044	2.766617078000503
4	0.7194069309272386	1.5695450279103698
5	0.41751810578650306	1.1386348929025685
6	0.2742479734442625	0.8974987408985766
7	0.18891177210474538	0.7212672825065893
8	0.15237987427019314	0.6649006667370527
9	0.11626525720675243	0.5707312289830843
>10	0.8190810692072398	9.019482880374298
>50	0.1340912788928171	5.210559595931035
>100	0.12879824545567534	14.247749056159325
>500	0.010251408159385479	3.814186301012193
>1k	0.004035128743587901	3.7867645130959295
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	3557	0.21123130570282955	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	3332	0.19786975276970148	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3170	0.1882494346578492	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	2959	0.17571926724056022	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	2721	0.16158571347129583	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	2714	0.16117002071337627	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	2515	0.14935246945252076	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	2481	0.14733339034262585	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2356	0.13991030537977692	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	2287	0.1358127624802843	No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	1934	0.11484997054519885	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	1865	0.11075242764570625	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	1782	0.10582349923037455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	2.9692339851395777E-5	0.0
16-17	0.0	0.0	0.0	5.9384679702791554E-5	0.0
18-19	0.0	0.0	0.0	5.9384679702791554E-5	2.9692339851395777E-5
20-21	0.0	0.0	0.0	5.9384679702791554E-5	5.9384679702791554E-5
22-23	0.0	0.0	0.0	5.9384679702791554E-5	5.9384679702791554E-5
24-25	0.0	0.0	0.0	2.0784637895977044E-4	5.9384679702791554E-5
26-27	0.0	0.0	0.0	4.4538509777093666E-4	8.907701955418733E-5
28-29	0.0	0.0	0.0	0.0016924633715295592	1.7815403910837466E-4
30-31	0.0	0.0	0.0	0.006176006689090322	1.7815403910837466E-4
32-33	0.0	0.0	0.0	0.01864678942667655	1.7815403910837466E-4
34-35	0.0	0.0	0.0	0.038540657127111716	1.7815403910837466E-4
36-37	0.0	0.0	0.0	0.06847053569731866	1.7815403910837466E-4
38-39	0.0	0.0	0.0	0.11045550424719229	1.7815403910837466E-4
40-41	0.0	0.0	0.0	0.1533015506527564	2.3753871881116622E-4
42-43	0.0	0.0	0.0	0.20024513995781312	2.3753871881116622E-4
44-45	0.0	0.0	0.0	0.2607581285749577	2.3753871881116622E-4
46-47	0.0	0.0	0.0	0.33106958934306296	2.3753871881116622E-4
48-49	0.0	0.0	0.0	0.4072304410618931	2.9692339851395777E-4
50-51	0.0	0.0	0.0	0.492150533036885	2.9692339851395777E-4
52-53	0.0	0.0	0.0	0.5734184672101552	2.9692339851395777E-4
54-55	0.0	0.0	0.0	0.6646333352336431	3.2661573836535355E-4
56-57	0.0	0.0	0.0	0.7745246850236589	3.563080782167493E-4
58-59	0.0	0.0	0.0	0.897421279668586	3.563080782167493E-4
60-61	0.0	0.0	0.0	1.0289286528704178	3.563080782167493E-4
62-63	0.0	0.0	0.0	1.1625738745415504	3.563080782167493E-4
64-65	0.0	0.0	0.0	1.3044735666913707	3.563080782167493E-4
66-67	0.0	0.0	0.0	1.4643965091309883	3.563080782167493E-4
68-69	0.0	0.0	0.0	1.6464996294395986	3.563080782167493E-4
70-71	0.0	0.0	0.0	1.8514658514337836	4.156927579195409E-4
72-73	0.0	0.0	0.0	2.0595200767725137	4.156927579195409E-4
74-75	0.0	0.0	0.0	2.2739581551792942	4.156927579195409E-4
76-77	0.0	0.0	0.0	2.509121486802349	4.4538509777093666E-4
78-79	0.0	0.0	0.0	2.7737099272181367	4.7507743762233243E-4
80-81	0.0	0.0	0.0	3.059142390209604	4.7507743762233243E-4
82-83	0.0	0.0	0.0	3.3635482583661136	4.7507743762233243E-4
84-85	0.0	0.0	0.0	3.6662616631510936	4.7507743762233243E-4
86-87	0.0	0.0	0.0	3.9861669327100318	4.7507743762233243E-4
88-89	0.0	0.0	0.0	4.339208853543128	4.7507743762233243E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	2735	0.0	58.003952	2
ACCTGGG	2800	0.0	56.319183	3
GTACCTG	2850	0.0	55.331123	1
CCTGGGG	2725	0.0	46.22589	4
CTGGGGG	2460	0.0	41.195404	5
TATCACG	160	1.8189894E-12	35.5166	2
GTACCCG	320	0.0	35.5166	1
GTACACG	245	0.0	30.926022	1
TGGGGGG	4845	0.0	28.149382	6
ATAACGC	250	0.0	26.519064	3
GTACTAG	1165	0.0	26.421507	1
AGGGCTA	1550	0.0	26.274649	5
TAGTACT	1380	0.0	25.736668	4
CTGTGCG	1355	0.0	25.165295	9
GCTATTG	1590	0.0	24.72015	8
GTATAAG	1055	0.0	24.687685	1
TAACGCA	270	1.8189894E-12	24.55469	4
GGGCTAT	1725	0.0	23.609102	6
CTATTGA	1680	0.0	23.39794	9
GCATCGT	1255	0.0	23.394733	8
>>END_MODULE