FastQCFastQC Report
Sat 18 Jun 2016
SRR1931077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683936
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44670.26527136423236986No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA38920.23112517340326474No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA38510.22869040153545026No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC36890.21907008342359804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35250.20933099595234023No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC31500.1870617410637934No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC29860.1773226535925356No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC27070.16075432795545674No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT26140.1552315527430971No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26080.15487524466488037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.15238108811736312No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA19870.11799735856944682No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT19180.1138998156699542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG27450.056.4093132
ACCTGGG27850.055.4285773
GTACCTG29250.053.144541
CCTGGGG25200.046.932574
CTGGGGG21050.044.677485
GTACCCG2800.044.142011
TATCACG1500.037.998422
ATAACGC2650.037.6399463
TAACGCA2900.034.3951234
TGGGGGG42600.031.8883466
GAACCGT2700.029.9061646
GTATAAC4500.029.578921
TAGTACT15250.028.9660074
GTACTAG10550.028.8379441
CTGTGCG15150.028.2166489
GTACACG3600.027.7302381
GGGGGGA44400.026.6374077
CTAGTAC17850.024.746873
AACCGTG2701.8189894E-1224.6286077
GTACTGT17650.024.2199556
Produced by FastQC (version 0.11.5)