##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931077_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1683936 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.642360517264315 33.0 33.0 33.0 33.0 33.0 2 32.59072494441594 33.0 33.0 33.0 33.0 33.0 3 32.64067042927997 33.0 33.0 33.0 33.0 33.0 4 36.59420726203371 37.0 37.0 37.0 37.0 37.0 5 36.544468435855045 37.0 37.0 37.0 37.0 37.0 6 36.54854103718906 37.0 37.0 37.0 37.0 37.0 7 36.48760760503962 37.0 37.0 37.0 37.0 37.0 8 36.49344214982042 37.0 37.0 37.0 37.0 37.0 9 36.4866521055432 37.0 37.0 37.0 37.0 37.0 10-11 36.46671429317979 37.0 37.0 37.0 37.0 37.0 12-13 36.371170578929366 37.0 37.0 37.0 37.0 37.0 14-15 38.30395216920358 40.0 37.0 40.0 37.0 40.0 16-17 38.30480998090189 40.0 37.0 40.0 37.0 40.0 18-19 38.269885850768674 40.0 37.0 40.0 37.0 40.0 20-21 38.18831416395872 40.0 37.0 40.0 35.0 40.0 22-23 38.045532015468524 40.0 37.0 40.0 33.0 40.0 24-25 38.205343611633694 40.0 37.0 40.0 37.0 40.0 26-27 38.142736719210234 40.0 37.0 40.0 35.0 40.0 28-29 38.05699207095756 40.0 37.0 40.0 33.0 40.0 30-31 37.91918309246907 40.0 37.0 40.0 33.0 40.0 32-33 37.783980507572736 40.0 37.0 40.0 33.0 40.0 34-35 37.68276050871292 40.0 37.0 40.0 33.0 40.0 36-37 37.60382936168595 40.0 37.0 40.0 33.0 40.0 38-39 37.44536906390742 40.0 37.0 40.0 33.0 40.0 40-41 37.256420671569465 40.0 37.0 40.0 33.0 40.0 42-43 37.054692399236075 40.0 37.0 40.0 33.0 40.0 44-45 36.7904213699333 40.0 37.0 40.0 33.0 40.0 46-47 36.486283326682255 38.5 37.0 40.0 33.0 40.0 48-49 36.183368607833074 37.0 37.0 40.0 33.0 40.0 50-51 35.901446076335446 37.0 37.0 40.0 33.0 40.0 52-53 35.66467015373506 37.0 37.0 40.0 30.0 40.0 54-55 35.416419032552305 37.0 37.0 40.0 27.0 40.0 56-57 35.090969609296316 37.0 35.0 40.0 27.0 40.0 58-59 34.79568760332934 37.0 33.0 40.0 27.0 40.0 60-61 34.53378008427873 37.0 33.0 40.0 27.0 40.0 62-63 34.21161374304012 37.0 33.0 40.0 27.0 40.0 64-65 33.852046039754484 37.0 33.0 37.0 27.0 40.0 66-67 33.490469946601294 37.0 33.0 37.0 27.0 40.0 68-69 33.17118881002604 37.0 33.0 37.0 22.0 40.0 70-71 32.88230966022462 37.0 33.0 37.0 22.0 40.0 72-73 32.54005556030633 37.0 33.0 37.0 22.0 40.0 74-75 32.14867429641032 37.0 33.0 37.0 22.0 37.0 76-77 30.51429864317884 33.0 30.0 37.0 18.5 37.0 78-79 31.304073610873573 33.0 33.0 37.0 22.0 37.0 80-81 31.367400542538434 33.0 33.0 37.0 18.5 37.0 82-83 31.139900209984226 33.0 33.0 37.0 15.0 37.0 84-85 30.836859298690687 33.0 33.0 37.0 15.0 37.0 86-87 30.5029778447637 33.0 33.0 37.0 15.0 37.0 88-89 30.172307617391635 33.0 33.0 37.0 10.5 37.0 90-91 29.74632141601581 33.0 27.0 37.0 6.0 37.0 92-93 29.335982186971478 33.0 27.0 37.0 2.0 37.0 94-95 28.8452485723923 33.0 27.0 37.0 2.0 37.0 96-97 28.34545612184786 33.0 27.0 37.0 2.0 37.0 98-99 27.667885834140964 33.0 27.0 37.0 2.0 37.0 100-101 25.950196147597058 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.12262732749451288 1101 2 0.15388887031193832 1101 3 0.060107669261164176 1101 4 0.04814195981311542 1101 5 0.0355406336644748 1101 6 0.021692535892391618 1101 7 -0.09156896382396695 1101 8 0.06727025193967506 1101 9 0.14111154531124726 1101 10-11 0.11264945718794905 1101 12-13 0.07420740749268617 1101 14-15 0.09263069406166125 1101 16-17 0.17041074202285245 1101 18-19 0.10289739004781495 1101 20-21 0.019654223460619846 1101 22-23 0.07104826198056458 1101 24-25 0.09572781952521581 1101 26-27 0.09456499852812073 1101 28-29 0.037824300160515634 1101 30-31 -0.09959853225316806 1101 32-33 0.0027821915741128578 1101 34-35 -0.04935209094093551 1101 36-37 0.04835771761517549 1101 38-39 -0.0034457496775459617 1101 40-41 -0.10302267264017217 1101 42-43 -0.18931812867404574 1101 44-45 -0.23665309595300243 1101 46-47 -0.4544817672712327 1101 48-49 -0.5554487482403303 1101 50-51 -0.3532570331984388 1101 52-53 -0.2595161867705471 1101 54-55 -0.32388257924500863 1101 56-57 -0.3019476110794912 1101 58-59 -0.4527688118523656 1101 60-61 -0.4600196730252222 1101 62-63 -0.6607220633839148 1101 64-65 -0.686428340128991 1101 66-67 -0.6056810492356703 1101 68-69 -0.7563620484260483 1101 70-71 -0.7931368629301758 1101 72-73 -0.9955155849611401 1101 74-75 -1.1849445317829712 1101 76-77 -1.1621211875472675 1101 78-79 -1.0758747763965104 1101 80-81 -1.2069262308273103 1101 82-83 -1.3277932415053577 1101 84-85 -1.565181233399656 1101 86-87 -1.628911328174894 1101 88-89 -1.963726139482148 1101 90-91 -2.038743502719523 1101 92-93 -2.064058044443012 1101 94-95 -2.3039486590957345 1101 96-97 -2.419957520946241 1101 98-99 -2.671466826419536 1101 100-101 -4.1357017507747535 1102 1 0.1589166652113576 1102 2 0.2640381374455387 1102 3 0.18711942551574623 1102 4 0.20408501588671868 1102 5 0.17913685950613 1102 6 0.14535596993442113 1102 7 0.15770749465615097 1102 8 0.1538655660154049 1102 9 0.14898588738924445 1102 10-11 0.17628053044696657 1102 12-13 0.16414240963396054 1102 14-15 0.309676745148181 1102 16-17 0.263482529774798 1102 18-19 0.2331305465538165 1102 20-21 0.14717625780379961 1102 22-23 0.18742879301513682 1102 24-25 0.18875989020088468 1102 26-27 0.29317369207806365 1102 28-29 0.1755484426170355 1102 30-31 0.35890230353226826 1102 32-33 0.37103978616983113 1102 34-35 0.3195340695574984 1102 36-37 0.23885117638539555 1102 38-39 0.26529293958986244 1102 40-41 0.248075418177244 1102 42-43 0.20499214112577846 1102 44-45 0.17876420964054773 1102 46-47 0.14951480669923 1102 48-49 0.1374691790118021 1102 50-51 0.12474747727996771 1102 52-53 -0.02708322228797755 1102 54-55 -0.030482175857656557 1102 56-57 0.04284323534320578 1102 58-59 -0.08542298318147346 1102 60-61 -0.1724317774085975 1102 62-63 -0.19577929051033038 1102 64-65 -0.20930192240205514 1102 66-67 -0.1572174093555816 1102 68-69 -0.2641232831781437 1102 70-71 -0.4449346650217123 1102 72-73 -0.6269540683904822 1102 74-75 -0.5814211090689483 1102 76-77 -0.6935140826803696 1102 78-79 -0.5948724900743798 1102 80-81 -0.470091578196314 1102 82-83 -0.4854778050319517 1102 84-85 -0.6775831494895606 1102 86-87 -0.6748570781023631 1102 88-89 -0.9686536471838068 1102 90-91 -0.7452472603003031 1102 92-93 -0.7653018796620437 1102 94-95 -0.751388289139804 1102 96-97 -0.9989116149133892 1102 98-99 -1.3010636462905083 1102 100-101 -1.9225272512099139 1103 1 0.14250399385122847 1103 2 0.2300324101651512 1103 3 0.10770914712829693 1103 4 0.165676070231477 1103 5 0.09029300056105427 1103 6 0.08947287383402625 1103 7 0.09630313842195193 1103 8 0.1317173569465524 1103 9 0.20839114532329006 1103 10-11 0.16517360304701612 1103 12-13 0.24067955190600543 1103 14-15 0.33945245143844716 1103 16-17 0.17565845042521033 1103 18-19 0.3059077160420145 1103 20-21 0.20118803770316163 1103 22-23 0.35104045599078404 1103 24-25 0.25237659014457847 1103 26-27 0.29409149371400645 1103 28-29 0.4523418096732854 1103 30-31 0.2590892080759488 1103 32-33 0.27929306474058535 1103 34-35 0.2655662599119566 1103 36-37 0.30411934126104256 1103 38-39 0.280198442766455 1103 40-41 0.1869955748021752 1103 42-43 0.3421097650640874 1103 44-45 0.4392144732969214 1103 46-47 0.31714283238942187 1103 48-49 0.3581017974259666 1103 50-51 0.3079510880428913 1103 52-53 0.04945041062109823 1103 54-55 0.06262184331254161 1103 56-57 -0.0976101413245658 1103 58-59 -0.0762383609623356 1103 60-61 0.0024199623271314863 1103 62-63 -0.02912273906636642 1103 64-65 -0.10862696868435506 1103 66-67 -0.0634389731937901 1103 68-69 -0.14359875314377746 1103 70-71 -0.11025394273700329 1103 72-73 0.01665711566032968 1103 74-75 -0.17179608243907651 1103 76-77 -0.33242416536921837 1103 78-79 -0.26748484336236444 1103 80-81 -0.5465746349918454 1103 82-83 -0.28984320485987425 1103 84-85 -0.1708036795107155 1103 86-87 -0.38573717679390995 1103 88-89 -0.4428651965386621 1103 90-91 -0.22146327521633324 1103 92-93 -0.25814670141117446 1103 94-95 -0.23702735652452844 1103 96-97 -0.3231858373624128 1103 98-99 -0.31077487938982884 1103 100-101 -0.9270126299651373 1104 1 0.016856799489616492 1104 2 0.17108463750770397 1104 3 0.10468438883788878 1104 4 0.10409434076549928 1104 5 0.09096391408775162 1104 6 0.05800205970191996 1104 7 0.14169823194323072 1104 8 0.06356654823596841 1104 9 0.08892900562870665 1104 10-11 0.1475039545424508 1104 12-13 0.10191904770432103 1104 14-15 0.12817963586060444 1104 16-17 0.1086382552503693 1104 18-19 0.24423336888379055 1104 20-21 0.2244491970055904 1104 22-23 0.16764217732448117 1104 24-25 0.22436538566277875 1104 26-27 0.21126473397784906 1104 28-29 0.20921980280602526 1104 30-31 0.2557916793870447 1104 32-33 0.1747067947487153 1104 34-35 0.32667304133420316 1104 36-37 0.20116856417601525 1104 38-39 0.2917592767774764 1104 40-41 0.17710298873549135 1104 42-43 -0.03187765248357266 1104 44-45 0.1030625125126079 1104 46-47 0.1152272274377637 1104 48-49 0.2553978126062262 1104 50-51 0.1752813265899249 1104 52-53 0.21925365449929046 1104 54-55 0.1467192726068447 1104 56-57 0.14801932013744334 1104 58-59 0.07967166433811457 1104 60-61 0.12367741692187195 1104 62-63 0.00359671968487163 1104 64-65 0.10550287680222681 1104 66-67 0.243359956055329 1104 68-69 -0.013670249484249553 1104 70-71 -0.01668183647514354 1104 72-73 -0.06727484422039964 1104 74-75 0.01340202906359167 1104 76-77 -0.07961457379065351 1104 78-79 0.13020987968814524 1104 80-81 0.05573911308803403 1104 82-83 0.042378716166602715 1104 84-85 -0.12576324398166605 1104 86-87 -0.10162958214314699 1104 88-89 -0.2262922770482838 1104 90-91 -0.11466942864544549 1104 92-93 -0.20403820317317312 1104 94-95 -0.1016338442809186 1104 96-97 -0.10653159502031428 1104 98-99 -0.1729879904407028 1104 100-101 -0.8535588936318952 1105 1 0.062169829689821654 1105 2 0.190610143672167 1105 3 0.12068088244720343 1105 4 0.09528029258090243 1105 5 0.05733932410924325 1105 6 -0.015308368982616116 1105 7 0.05170134245108926 1105 8 0.061041381399640215 1105 9 0.06435740739321005 1105 10-11 0.023072329160704896 1105 12-13 0.05622144562311604 1105 14-15 0.0745339792606643 1105 16-17 0.06485220163651917 1105 18-19 0.05797333042661279 1105 20-21 -0.0081793235237555 1105 22-23 -0.06746909402491497 1105 24-25 -0.05491580424785525 1105 26-27 0.08388166079608794 1105 28-29 0.02947698525144915 1105 30-31 0.018358838079507223 1105 32-33 0.07645645542058332 1105 34-35 0.020451318678389896 1105 36-37 0.051023642787029644 1105 38-39 0.17089271756471902 1105 40-41 0.12048538326099845 1105 42-43 0.1764281104391756 1105 44-45 0.04624837949597804 1105 46-47 0.16272468247679228 1105 48-49 0.12515236525864282 1105 50-51 0.06966756683424791 1105 52-53 -0.009723553961286768 1105 54-55 0.11418689504773738 1105 56-57 0.18812861207273812 1105 58-59 0.32345135554950843 1105 60-61 0.18952191980616107 1105 62-63 -0.06487050687321272 1105 64-65 -0.009269207803590973 1105 66-67 0.1335696325625122 1105 68-69 -0.1538417515767705 1105 70-71 -0.19774874067149995 1105 72-73 -0.2515300189743854 1105 74-75 -0.08165367730446604 1105 76-77 -0.25428726353163356 1105 78-79 -0.0512302854515454 1105 80-81 -0.25482176803400947 1105 82-83 -0.2865970784267695 1105 84-85 -0.16768582061326853 1105 86-87 -0.2197334448283641 1105 88-89 -0.24918985650761982 1105 90-91 -0.25615936191091393 1105 92-93 -0.41354009775523437 1105 94-95 -0.34552028246664435 1105 96-97 -0.23488387417425471 1105 98-99 -0.4070155325739435 1105 100-101 -1.2087916161745937 1106 1 0.07413471065407862 1106 2 0.16923389721158344 1106 3 0.13632704590071398 1106 4 0.1502828161556522 1106 5 0.1601015691497807 1106 6 0.16459969875754155 1106 7 0.14166321793762648 1106 8 0.11391668829199375 1106 9 0.12813968990241165 1106 10-11 0.14294212981256038 1106 12-13 0.15614573838059442 1106 14-15 0.17826216887380752 1106 16-17 0.15760284108470302 1106 18-19 0.16924337288539704 1106 20-21 0.06455273842215803 1106 22-23 0.08465648304676421 1106 24-25 0.11051984744749888 1106 26-27 0.26576403369774226 1106 28-29 0.17089697367436685 1106 30-31 0.33167953452910126 1106 32-33 0.26404503004283697 1106 34-35 0.2568175991573298 1106 36-37 0.19108703156297224 1106 38-39 0.24532320234770566 1106 40-41 0.202885801860738 1106 42-43 0.19486054279453668 1106 44-45 0.23953460134814009 1106 46-47 0.07219001255181468 1106 48-49 0.1788171803533274 1106 50-51 0.17332304327660353 1106 52-53 0.29013700784062735 1106 54-55 0.29947787604821485 1106 56-57 0.17583794758841975 1106 58-59 0.22172598606680083 1106 60-61 0.23842081428081485 1106 62-63 0.2615774119617811 1106 64-65 0.3343244054136605 1106 66-67 0.34906473796809223 1106 68-69 0.3869424956137877 1106 70-71 0.4175668630046445 1106 72-73 0.4883974246871716 1106 74-75 0.3480408846058083 1106 76-77 0.3860341857131466 1106 78-79 0.5638283270670996 1106 80-81 0.49553253045498025 1106 82-83 0.5806340182874514 1106 84-85 0.7686495211243738 1106 86-87 0.753024779601553 1106 88-89 0.6542349338360687 1106 90-91 0.7559438166526711 1106 92-93 0.7716845859424737 1106 94-95 0.931659473046011 1106 96-97 0.8257963361521554 1106 98-99 1.0791778759058417 1106 100-101 0.9021383852796703 1107 1 0.07210056366194806 1107 2 0.14359030644194348 1107 3 0.10848257477893242 1107 4 0.06684944280631555 1107 5 0.07163284832811456 1107 6 0.033880744509176 1107 7 0.09314381017452433 1107 8 0.08720600401828449 1107 9 0.019229459143446093 1107 10-11 0.11694839898689935 1107 12-13 0.08516734702406126 1107 14-15 0.12527430706241205 1107 16-17 0.2047550352957188 1107 18-19 0.20319028498357028 1107 20-21 0.15972414031624993 1107 22-23 0.1962844675739106 1107 24-25 0.15378715437026358 1107 26-27 0.22924942785540736 1107 28-29 0.19650721777200886 1107 30-31 0.24327750705144524 1107 32-33 0.22004409633601796 1107 34-35 0.114243903012202 1107 36-37 0.1435013306159263 1107 38-39 0.15296959423779555 1107 40-41 0.19653326084434042 1107 42-43 0.30061951304930545 1107 44-45 0.1539837143266638 1107 46-47 0.20235874671214304 1107 48-49 0.29054520489648183 1107 50-51 0.3794054762497936 1107 52-53 0.23736589939725405 1107 54-55 0.410055440407298 1107 56-57 0.4694053745592086 1107 58-59 0.4532686031136208 1107 60-61 0.48877095433684303 1107 62-63 0.47141134553521624 1107 64-65 0.571035756620816 1107 66-67 0.5678355796380998 1107 68-69 0.5634130838490847 1107 70-71 0.5721787077425375 1107 72-73 0.758972321312477 1107 74-75 0.6709700562744771 1107 76-77 0.5815957436696628 1107 78-79 0.9309751858105955 1107 80-81 0.9141999974417807 1107 82-83 0.965054453128058 1107 84-85 0.9365666879911245 1107 86-87 0.9796487625280577 1107 88-89 0.9113211016365241 1107 90-91 1.0473770566153249 1107 92-93 1.3575689396839685 1107 94-95 1.4254069720456108 1107 96-97 1.3387348448889789 1107 98-99 1.5188203995819762 1107 100-101 2.073340199338624 1108 1 0.04347848971846702 1108 2 0.11302858293716156 1108 3 0.11176150618017289 1108 4 0.055490497916160564 1108 5 0.045181024660799096 1108 6 0.06432251844857007 1108 7 0.06725051135691018 1108 8 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-0.028529662562107205 2316 72-73 -0.1850077634750491 2316 74-75 -0.21932571420763125 2316 76-77 -0.41355608517781306 2316 78-79 -0.10102718035326319 2316 80-81 -0.07307299871320794 2316 82-83 -0.007227909092197393 2316 84-85 0.013817501359952189 2316 86-87 0.07715147375747478 2316 88-89 0.09947894448380623 2316 90-91 0.31661939123032923 2316 92-93 0.3299395400980494 2316 94-95 0.3508713317025176 2316 96-97 0.5950574318947979 2316 98-99 0.9890254671162353 2316 100-101 1.1575953506745265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 12.0 5 37.0 6 72.0 7 154.0 8 400.0 9 698.0 10 1374.0 11 2053.0 12 3301.0 13 4785.0 14 5740.0 15 6964.0 16 9040.0 17 12306.0 18 12589.0 19 9394.0 20 8372.0 21 8253.0 22 8623.0 23 9731.0 24 11252.0 25 13146.0 26 15826.0 27 19793.0 28 24179.0 29 29984.0 30 37550.0 31 47655.0 32 62395.0 33 83679.0 34 119325.0 35 184484.0 36 297029.0 37 444968.0 38 188752.0 39 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.00690621690276 21.737347418370536 13.665572687041394 24.59017367768531 2 14.21878767220093 21.44911855215228 40.42812841833469 23.903965357312103 3 17.42809702981586 27.944054880945597 29.922811793322314 24.705036295916237 4 11.858110997092526 17.648829884271137 38.676291735552894 31.816767383083445 5 12.498990460445054 37.94633525264618 34.86118237272676 14.693491914182012 6 29.038870835946256 38.32253719856337 18.964675617125593 13.673916348364784 7 26.58824325865116 31.939396746669708 23.804348858863992 17.66801113581514 8 26.221245938087907 34.7599314938335 20.419006423046955 18.59981614503164 9 26.56609277312202 15.221124793341314 21.844773198031277 36.36800923550539 10-11 25.411298291621538 26.189059441688993 28.128325542063354 20.271316724626114 12-13 25.526563954924654 24.278060448853164 28.33747838397659 21.857897212245597 14-15 22.500849202936934 25.50539451711827 26.609714644059263 25.384041635885534 16-17 21.447816306557968 27.606809284913442 27.645528096079662 23.29984631244893 18-19 21.2431470079623 27.742146969956107 29.586813275563916 21.42789274651768 20-21 22.364359453090856 26.84769492427266 28.83378584459267 21.95415977804382 22-23 22.45091262375767 26.78842901392927 28.528014128802997 22.23264423351006 24-25 22.12557365600593 26.842765995857327 29.306072202268968 21.72558814586778 26-27 22.135164281777932 26.86797479239116 28.719321874465535 22.277539051365373 28-29 21.546899644642075 27.424142010147655 29.020134969500027 22.00882337571024 30-31 22.342476198620375 26.937365790623875 28.701951855652467 22.018206155103282 32-33 22.689460882123786 27.225648718241075 28.120100763924523 21.96478963571062 34-35 22.421160899226575 26.81245011686905 28.996440482298613 21.76994850160576 36-37 22.459018632537102 26.609799897383272 28.85151217148395 22.07966929859567 38-39 22.383778243353667 26.788963476046597 29.062743477186782 21.764514803412958 40-41 22.082978005715333 26.344296427561865 29.22245013338148 22.35027543334133 42-43 22.26295893009377 26.624752761308397 28.50006992909398 22.612218379503858 44-45 22.112330872432207 27.28135154780229 28.40600830435361 22.200309275411893 46-47 22.383481319955152 26.99832416433879 28.1292756969386 22.48891881876746 48-49 22.929520990727664 26.60902980758153 28.175213517869462 22.28623568382134 50-51 22.368991458107672 27.091944111890236 27.739801275107844 22.79926315489425 52-53 22.081153915588242 27.46868051992475 27.544752294624026 22.905413269862986 54-55 22.19704311802824 27.29426771563765 27.53156889573 22.97712027060411 56-57 23.04170110978089 26.82910751952568 27.336787146304847 22.792404224388576 58-59 22.497321750945403 27.103613201451836 27.87656419246337 22.522500855139388 60-61 22.498034367101837 27.70253738859434 27.35772024590008 22.44170799840374 62-63 22.63099666495639 27.072465936947722 27.10545412652262 23.191083271573266 64-65 21.822177327404365 27.4655331319005 27.45849604735572 23.253793493339415 66-67 22.3899445394949 26.83058564606827 27.243733449888087 23.535736364548747 68-69 22.028282053231216 27.016006423474188 27.304308526306787 23.65140299698781 70-71 22.175133022946458 26.74114566012637 27.542816760891252 23.540904556035915 72-73 22.67298163350626 26.78219362256048 27.70951508845942 22.835309655473843 74-75 22.566191687921446 27.453464652733032 27.16731940365869 22.813024255686827 76-77 22.498504223064508 27.04142003049455 27.19757468863343 23.262501057807512 78-79 22.046572590283724 27.308548978245913 27.3750634337078 23.269814997762563 80-81 21.81699623157843 27.317508001697288 27.851221730528312 23.01427403619597 82-83 22.410071152038263 26.53250658160808 28.53501985617561 22.52240241017805 84-85 22.585124991723042 27.098212681755328 27.273491195840112 23.043171130681518 86-87 21.7347049667771 27.530584721802402 27.572332325175232 23.162377986245264 88-89 22.247371634076355 26.754104669061057 27.420341390646673 23.578182306215915 90-91 22.103660709195598 26.608344972730556 27.800848725842314 23.487145592231535 92-93 22.411935264049458 26.91747071931968 27.518420587706938 23.152173428923923 94-95 21.250456163334533 26.938059477463227 28.008770213343396 23.80271414585884 96-97 21.3233045288776 27.264602113092177 27.53273238105023 23.879360976979992 98-99 21.139847357619292 27.74805574558653 27.123269530433458 23.988827366360717 100-101 19.806518430365102 28.082589984280848 27.248322825195537 24.86256876015851 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 138.0 1 92.5 2 79.5 3 115.5 4 97.5 5 91.0 6 147.5 7 290.5 8 449.5 9 554.5 10 594.0 11 614.5 12 736.5 13 862.5 14 983.0 15 1191.5 16 1361.5 17 1523.0 18 1736.5 19 1950.5 20 2209.5 21 2472.0 22 2841.5 23 3263.5 24 3759.0 25 4462.0 26 5660.0 27 7307.0 28 9358.5 29 13146.0 30 18290.0 31 22770.5 32 28346.0 33 34051.0 34 38411.0 35 43137.5 36 46896.5 37 52802.0 38 55489.5 39 52744.5 40 50418.5 41 48330.5 42 48128.5 43 50035.5 44 54118.5 45 60954.5 46 66912.5 47 72005.0 48 77510.5 49 75499.0 50 69914.5 51 67712.0 52 64815.0 53 61617.5 54 58830.0 55 53444.0 56 46810.5 57 38654.5 58 31649.5 59 29279.0 60 24744.0 61 18284.5 62 13787.0 63 10839.0 64 8092.5 65 5270.0 66 3531.5 67 2492.0 68 1994.5 69 1608.0 70 1190.0 71 933.0 72 742.0 73 586.0 74 507.5 75 402.5 76 322.0 77 245.5 78 180.0 79 140.5 80 92.5 81 66.5 82 53.5 83 42.5 84 41.0 85 38.0 86 30.5 87 21.0 88 22.5 89 18.0 90 9.5 91 5.5 92 2.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08278224350569142 2 1.1876935940558311E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.3753871881116622E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0026129259069228284 42-43 0.005196159473994261 44-45 0.0 46-47 0.0 48-49 1.1876935940558311E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0020784637895977045 68-69 0.006651084126712654 70-71 9.501548752446649E-4 72-73 0.0 74-75 5.9384679702791554E-5 76-77 0.0016924633715295595 78-79 0.005433698192805427 80-81 0.004424158637857971 82-83 0.00457262033711495 84-85 0.002642618246774224 86-87 4.156927579195409E-4 88-89 0.0 90-91 0.0 92-93 0.0010986165745016437 94-95 0.00323646504380214 96-97 0.0031473880242479523 98-99 0.0 100-101 1.7815403910837466E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1683936.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.90815411232675 #Duplication Level Percentage of deduplicated Percentage of total 1 85.56673089267734 40.99344130494376 2 8.684614537071928 8.321277032963902 3 2.077362420880346 2.985677970200754 4 0.9062798967082796 1.736727878416153 5 0.5115684856565805 1.2254150924922538 6 0.34247379584569726 0.9844372434485514 7 0.23884485398080205 0.8009831251413909 8 0.16820679682847542 0.6446781716159546 9 0.14714895216097226 0.634467120983573 >10 1.006800643339094 9.794413209017156 >50 0.15853217538390876 5.378053057851157 >100 0.17198117424097925 17.043969853616638 >500 0.013469105925405548 4.424529631473876 >1k 0.005986269300180244 5.031929307834907 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4467 0.26527136423236986 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 3892 0.23112517340326474 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 3851 0.22869040153545026 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 3689 0.21907008342359804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3525 0.20933099595234023 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 3150 0.1870617410637934 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 2986 0.1773226535925356 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2707 0.16075432795545674 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2614 0.1552315527430971 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2608 0.15487524466488037 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2566 0.15238108811736312 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1987 0.11799735856944682 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 1918 0.1138998156699542 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 2.9692339851395777E-5 0.0 16-17 0.0 0.0 0.0 5.9384679702791554E-5 0.0 18-19 0.0 0.0 0.0 5.9384679702791554E-5 5.9384679702791554E-5 20-21 0.0 0.0 0.0 5.9384679702791554E-5 5.9384679702791554E-5 22-23 0.0 0.0 0.0 5.9384679702791554E-5 5.9384679702791554E-5 24-25 0.0 0.0 0.0 1.1876935940558311E-4 5.9384679702791554E-5 26-27 0.0 0.0 0.0 2.9692339851395777E-4 5.9384679702791554E-5 28-29 0.0 0.0 0.0 0.0015440016722725804 5.9384679702791554E-5 30-31 0.0 0.0 0.0 0.005938467970279156 5.9384679702791554E-5 32-33 0.0 0.0 0.0 0.018201404328905612 5.9384679702791554E-5 34-35 0.0 0.0 0.0 0.03803588734963799 5.9384679702791554E-5 36-37 0.0 0.0 0.0 0.06793607357999354 5.9384679702791554E-5 38-39 0.0 0.0 0.0 0.10995073446971856 5.9384679702791554E-5 40-41 0.0 0.0 0.0 0.15306401193394523 5.9384679702791554E-5 42-43 0.0 0.0 0.0 0.19997790889915057 5.9384679702791554E-5 44-45 0.0 0.0 0.0 0.26019397411778117 5.9384679702791554E-5 46-47 0.0 0.0 0.0 0.3302382038272238 1.1876935940558311E-4 48-49 0.0 0.0 0.0 0.4058942857685803 1.1876935940558311E-4 50-51 0.0 0.0 0.0 0.48968606882921917 1.1876935940558311E-4 52-53 0.0 0.0 0.0 0.5697663094084335 1.1876935940558311E-4 54-55 0.0 0.0 0.0 0.6597934838378656 1.1876935940558311E-4 56-57 0.0 0.0 0.0 0.7681111396157574 1.1876935940558311E-4 58-59 0.0 0.0 0.0 0.8888698857913839 1.1876935940558311E-4 60-61 0.0 0.0 0.0 1.0193677194382684 1.1876935940558311E-4 62-63 0.0 0.0 0.0 1.1526863253710355 1.1876935940558311E-4 64-65 0.0 0.0 0.0 1.2934280162666516 1.1876935940558311E-4 66-67 0.0 0.0 0.0 1.4513615719362256 1.1876935940558311E-4 68-69 0.0 0.0 0.0 1.630703304638656 1.1876935940558311E-4 70-71 0.0 0.0 0.0 1.832284599889782 1.1876935940558311E-4 72-73 0.0 0.0 0.0 2.0378149763411435 1.1876935940558311E-4 74-75 0.0 0.0 0.0 2.2496104365011496 1.1876935940558311E-4 76-77 0.0 0.0 0.0 2.481656072439808 1.1876935940558311E-4 78-79 0.0 0.0 0.0 2.7413749691199665 1.1876935940558311E-4 80-81 0.0 0.0 0.0 3.019384347148585 1.1876935940558311E-4 82-83 0.0 0.0 0.0 3.31624836098284 1.7815403910837466E-4 84-85 0.0 0.0 0.0 3.611271449746309 1.7815403910837466E-4 86-87 0.0 0.0 0.0 3.92295194116641 1.7815403910837466E-4 88-89 0.0 0.0 0.0 4.265987543469586 1.7815403910837466E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2745 0.0 56.409313 2 ACCTGGG 2785 0.0 55.428577 3 GTACCTG 2925 0.0 53.14454 1 CCTGGGG 2520 0.0 46.93257 4 CTGGGGG 2105 0.0 44.67748 5 GTACCCG 280 0.0 44.14201 1 TATCACG 150 0.0 37.99842 2 ATAACGC 265 0.0 37.639946 3 TAACGCA 290 0.0 34.395123 4 TGGGGGG 4260 0.0 31.888346 6 GAACCGT 270 0.0 29.906164 6 GTATAAC 450 0.0 29.57892 1 TAGTACT 1525 0.0 28.966007 4 GTACTAG 1055 0.0 28.837944 1 CTGTGCG 1515 0.0 28.216648 9 GTACACG 360 0.0 27.730238 1 GGGGGGA 4440 0.0 26.637407 7 CTAGTAC 1785 0.0 24.74687 3 AACCGTG 270 1.8189894E-12 24.628607 7 GTACTGT 1765 0.0 24.219955 6 >>END_MODULE