##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931076_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1218331 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.301402492426114 33.0 33.0 33.0 27.0 33.0 2 31.21740643552532 33.0 33.0 33.0 27.0 33.0 3 31.240776931720525 33.0 33.0 33.0 27.0 33.0 4 34.867756791873475 37.0 37.0 37.0 33.0 37.0 5 34.835078480314465 37.0 37.0 37.0 33.0 37.0 6 34.942194690933746 37.0 37.0 37.0 33.0 37.0 7 34.902963972844816 37.0 37.0 37.0 33.0 37.0 8 34.91513636277826 37.0 37.0 37.0 33.0 37.0 9 34.935584828753434 37.0 37.0 37.0 33.0 37.0 10-11 34.83271541149327 37.0 37.0 37.0 33.0 37.0 12-13 34.736004008762805 37.0 37.0 37.0 33.0 37.0 14-15 36.38537720865676 40.0 37.0 40.0 33.0 40.0 16-17 36.32915110918133 40.0 37.0 40.0 33.0 40.0 18-19 36.256396660677595 40.0 37.0 40.0 33.0 40.0 20-21 36.16927870997291 40.0 37.0 40.0 33.0 40.0 22-23 36.058707773174945 40.0 37.0 40.0 33.0 40.0 24-25 35.91961831390648 40.0 37.0 40.0 33.0 40.0 26-27 35.736365158565285 40.0 37.0 40.0 27.0 40.0 28-29 35.61289050348387 40.0 37.0 40.0 27.0 40.0 30-31 35.450778975500086 37.0 37.0 40.0 27.0 40.0 32-33 35.27863487016255 37.0 37.0 40.0 27.0 40.0 34-35 35.12941967330717 37.0 37.0 40.0 27.0 40.0 36-37 34.88783179612109 37.0 37.0 40.0 27.0 40.0 38-39 34.65389413878495 37.0 37.0 40.0 24.5 40.0 40-41 34.40179269837179 37.0 35.0 40.0 22.0 40.0 42-43 34.17018815083914 37.0 33.0 40.0 22.0 40.0 44-45 33.82214151983328 37.0 33.0 40.0 22.0 40.0 46-47 33.62135289999188 37.0 33.0 40.0 15.0 40.0 48-49 33.54961090212758 37.0 33.0 40.0 15.0 40.0 50-51 32.70774690950161 37.0 33.0 38.5 15.0 40.0 52-53 32.83514865828744 37.0 33.0 38.5 15.0 40.0 54-55 33.27899191598999 37.0 33.0 40.0 15.0 40.0 56-57 33.17763973829772 37.0 33.0 40.0 15.0 40.0 58-59 32.96375984851407 37.0 33.0 40.0 15.0 40.0 60-61 32.956406346058664 37.0 33.0 40.0 15.0 40.0 62-63 32.74846449774323 37.0 33.0 40.0 6.0 40.0 64-65 32.499608480782314 37.0 33.0 40.0 6.0 40.0 66-67 32.23738007158974 37.0 33.0 38.5 6.0 40.0 68-69 31.97084577179765 37.0 33.0 37.0 2.0 40.0 70-71 31.755835647291253 37.0 33.0 37.0 2.0 40.0 72-73 31.183375043399536 37.0 33.0 37.0 2.0 40.0 74-75 31.005038450142038 37.0 33.0 37.0 2.0 40.0 76-77 30.66626844428977 37.0 33.0 37.0 2.0 38.5 78-79 30.420035277769344 37.0 33.0 37.0 2.0 37.0 80-81 30.20126180816215 37.0 33.0 37.0 2.0 37.0 82-83 29.951224256790642 37.0 33.0 37.0 2.0 37.0 84-85 29.573972918689584 33.0 30.0 37.0 2.0 37.0 86-87 29.400338660019322 33.0 27.0 37.0 2.0 37.0 88-89 29.223932166217555 33.0 27.0 37.0 2.0 37.0 90-91 28.923313533021812 33.0 27.0 37.0 2.0 37.0 92-93 28.61948805373909 33.0 27.0 37.0 2.0 37.0 94-95 28.359185229629716 33.0 27.0 37.0 2.0 37.0 96-97 28.044349606141516 33.0 27.0 37.0 2.0 37.0 98-99 27.69019420830628 33.0 27.0 37.0 2.0 37.0 100-101 26.964816211686315 33.0 24.5 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.06689238238759287 1101 2 -0.19033092209511793 1101 3 -0.036404832877202864 1101 4 -0.16764694985565143 1101 5 -0.1851328666948291 1101 6 -0.11860982577324819 1101 7 -0.10423483871174 1101 8 -0.24075200696530885 1101 9 0.04246493907091775 1101 10-11 -0.1355062944639016 1101 12-13 0.019716620002668606 1101 14-15 -0.1378135548330235 1101 16-17 -0.04155702633774894 1101 18-19 0.08360616640332097 1101 20-21 -0.02207327528192593 1101 22-23 -0.10388655808788627 1101 24-25 -0.17640134916421601 1101 26-27 -0.22511322841903336 1101 28-29 -0.11740765940093212 1101 30-31 -0.16574390621370583 1101 32-33 -0.1437259627282259 1101 34-35 -0.09249953175992687 1101 36-37 0.037807199529211744 1101 38-39 -0.01980702565932546 1101 40-41 -0.09974030931209654 1101 42-43 -0.02192858196076486 1101 44-45 0.03724136613087836 1101 46-47 0.07369620515991215 1101 48-49 -0.14090172255729527 1101 50-51 -0.010582311295756597 1101 52-53 -0.1094873213140346 1101 54-55 0.2416837471447053 1101 56-57 0.19769226289411534 1101 58-59 0.3809097455660364 1101 60-61 0.41647699881443856 1101 62-63 0.36026500838450914 1101 64-65 0.12944499096288098 1101 66-67 0.26415596936359265 1101 68-69 0.26395597206350985 1101 70-71 0.12136701124076055 1101 72-73 0.1454290756792389 1101 74-75 0.11852390132586876 1101 76-77 -0.026110385112041 1101 78-79 0.052788006176211155 1101 80-81 0.16883721031684829 1101 82-83 0.42351765364773186 1101 84-85 0.3266357100583832 1101 86-87 0.253782018040944 1101 88-89 0.37774666513182 1101 90-91 0.5238978561393672 1101 92-93 0.40707749188308284 1101 94-95 0.16511212995077074 1101 96-97 -0.02522000295365956 1101 98-99 0.008802425333669817 1101 100-101 0.25732675391537185 1102 1 -0.1660957679024122 1102 2 -0.28767995192501417 1102 3 -0.24560262281956824 1102 4 -0.1286110600241912 1102 5 -0.42084887165732 1102 6 -0.3482550602881034 1102 7 -0.18195834623016083 1102 8 -0.33723886975305106 1102 9 -0.31981548501620694 1102 10-11 -0.3334200669245533 1102 12-13 -0.2232418976891637 1102 14-15 -0.1649710427228186 1102 16-17 -0.23756420229179298 1102 18-19 -0.24295275776081127 1102 20-21 -0.2489640641028501 1102 22-23 -0.21566488983335574 1102 24-25 -0.23588352266101964 1102 26-27 -0.21767103966017487 1102 28-29 0.009377332081875522 1102 30-31 -0.19414898231890731 1102 32-33 -0.03842201014896318 1102 34-35 -0.08920835999443 1102 36-37 -0.32794394996235354 1102 38-39 -0.2580716087814423 1102 40-41 -0.34481167801389034 1102 42-43 -0.2601858126709331 1102 44-45 -0.20306409175798734 1102 46-47 -0.32778882726422154 1102 48-49 -0.20043458899957045 1102 50-51 -0.09039003411446345 1102 52-53 -0.23204390199359182 1102 54-55 -0.15480744969638494 1102 56-57 -0.2393749605952067 1102 58-59 -0.27352650631366515 1102 60-61 -0.11614100933203275 1102 62-63 -0.18973564946287524 1102 64-65 -0.22180139764846274 1102 66-67 -0.27027460181047047 1102 68-69 -0.16571704682909072 1102 70-71 -0.24550209183463778 1102 72-73 -0.2983872750013994 1102 74-75 -0.21804668608541178 1102 76-77 -0.15248170668100158 1102 78-79 -0.1929907419479555 1102 80-81 -0.5145702533873902 1102 82-83 -0.4098047155625366 1102 84-85 -0.4360768036693443 1102 86-87 -0.5982839710171213 1102 88-89 -0.4967925514725593 1102 90-91 -0.3857235875183278 1102 92-93 -0.46573313397744087 1102 94-95 -0.520541302116424 1102 96-97 -0.38671178411801677 1102 98-99 -0.5791718923044584 1102 100-101 -0.5938387581646403 1103 1 -0.09837663912560757 1103 2 -0.12473242269858176 1103 3 -0.1941223678548809 1103 4 -0.29972892379611693 1103 5 -0.3807509171648604 1103 6 -0.21578402682055753 1103 7 -0.19587397743669754 1103 8 -0.31181604032078525 1103 9 -0.3336018867552397 1103 10-11 -0.2842161276638464 1103 12-13 -0.21478025537756196 1103 14-15 -0.21365048361405314 1103 16-17 -0.27262509536127055 1103 18-19 -0.27874354463830997 1103 20-21 -0.3357062758615825 1103 22-23 -0.3275166037120911 1103 24-25 -0.45596960825712785 1103 26-27 -0.21377141413685052 1103 28-29 -0.16801032672056238 1103 30-31 -0.24933249177251327 1103 32-33 -0.2442639762273089 1103 34-35 -0.4514381964072456 1103 36-37 -0.37534734010611004 1103 38-39 -0.5020478190081832 1103 40-41 -0.396185445009543 1103 42-43 -0.4351148628054702 1103 44-45 -0.42652566548412096 1103 46-47 -0.5536397022343564 1103 48-49 -0.6107547532004105 1103 50-51 -0.631090457828293 1103 52-53 -0.29244062923928027 1103 54-55 -0.30187274956289656 1103 56-57 -0.3559590720126735 1103 58-59 -0.3735104067766528 1103 60-61 -0.36842869578524784 1103 62-63 -0.14696972012612264 1103 64-65 -0.28655907795786106 1103 66-67 -0.16130131188091212 1103 68-69 -0.12038132816524794 1103 70-71 -0.05762771442828196 1103 72-73 -0.23306141820642168 1103 74-75 -0.2568317702614422 1103 76-77 -0.44708157543006877 1103 78-79 0.003132662808120301 1103 80-81 0.06694755031810473 1103 82-83 0.16290418675948004 1103 84-85 -0.04911143644467941 1103 86-87 -0.19238829168524418 1103 88-89 -0.3411890823472099 1103 90-91 -0.19096972633676756 1103 92-93 -0.29976712204934586 1103 94-95 -0.39416571219112484 1103 96-97 -0.42202933124164943 1103 98-99 -0.43395948309898813 1103 100-101 -0.4725453516830953 1104 1 0.06242496111146423 1104 2 0.1097961494355637 1104 3 0.0033204631818151142 1104 4 0.10397285918407562 1104 5 0.20946615203057206 1104 6 -0.0037711360219248036 1104 7 0.2595103593374759 1104 8 0.02121683697790644 1104 9 0.025959612067630644 1104 10-11 0.013647959196759984 1104 12-13 0.07639765575330415 1104 14-15 0.07282920061899034 1104 16-17 0.02932395881959593 1104 18-19 0.036306215820026466 1104 20-21 0.041844513555787444 1104 22-23 0.07233584356404066 1104 24-25 0.048372598818815504 1104 26-27 0.17760241079194117 1104 28-29 0.05591996747940442 1104 30-31 0.22369540536450927 1104 32-33 0.18639344627150223 1104 34-35 0.1895363924207345 1104 36-37 0.3154412129305584 1104 38-39 0.1030798953857186 1104 40-41 0.33615658920128766 1104 42-43 0.14052700183290767 1104 44-45 0.24124301629588984 1104 46-47 0.07487830866437406 1104 48-49 -0.16443348749849207 1104 50-51 -0.027727408158384037 1104 52-53 0.1203900781906242 1104 54-55 0.1987919579657884 1104 56-57 0.10357402754117118 1104 58-59 0.01945521776031711 1104 60-61 0.03911830706291397 1104 62-63 0.1871168112118511 1104 64-65 0.1025242841863232 1104 66-67 -0.005582769216154304 1104 68-69 0.08269814039799073 1104 70-71 0.20805362107821423 1104 72-73 0.1823838741002568 1104 74-75 0.011053595471754107 1104 76-77 0.010888535368238195 1104 78-79 0.36722055237200735 1104 80-81 0.32037809969990505 1104 82-83 0.18718041698289056 1104 84-85 0.2455268638796504 1104 86-87 0.39728754506423414 1104 88-89 0.4613145807469117 1104 90-91 0.4005469181043857 1104 92-93 0.3657501973889268 1104 94-95 0.33649397401753234 1104 96-97 0.24379256006734806 1104 98-99 0.08091000373856616 1104 100-101 0.31884761188351973 1105 1 -0.20148727044782433 1105 2 -0.06214596074184087 1105 3 -0.14871034914311565 1105 4 -8.824162594365248E-4 1105 5 -0.07795214862301947 1105 6 -0.1896955057698193 1105 7 -0.2159331514001508 1105 8 -0.16220986703703488 1105 9 -0.06019818438428359 1105 10-11 -0.15993980924394435 1105 12-13 -0.0069706915856286855 1105 14-15 -0.15106202253692658 1105 16-17 -0.0352535388461348 1105 18-19 0.031089128985293257 1105 20-21 0.053060666683705904 1105 22-23 -0.032881243270686866 1105 24-25 -0.13946586990200416 1105 26-27 -0.013305619086672493 1105 28-29 -0.097838202455236 1105 30-31 -0.04062532292680032 1105 32-33 -0.08731103552121766 1105 34-35 0.012213796715784042 1105 36-37 0.05532216531150169 1105 38-39 -0.001250169973758375 1105 40-41 0.05449225680165881 1105 42-43 0.018526534979500298 1105 44-45 0.18628269883557635 1105 46-47 0.01905431239920574 1105 48-49 0.06336763294968506 1105 50-51 -0.005341787243352769 1105 52-53 0.1029064180599093 1105 54-55 0.26188719606102495 1105 56-57 0.3278270620883248 1105 58-59 0.2775551243896359 1105 60-61 0.25897124823938356 1105 62-63 0.2515659241903734 1105 64-65 0.2272558434767049 1105 66-67 0.3796646630901037 1105 68-69 0.14243203395541215 1105 70-71 0.21213858839847788 1105 72-73 0.15807248287710252 1105 74-75 0.26593221358566765 1105 76-77 0.15420086029821434 1105 78-79 0.2615599547996439 1105 80-81 0.5179271193077497 1105 82-83 0.5821267288409651 1105 84-85 0.3785333998273579 1105 86-87 0.49336597643678104 1105 88-89 0.5532613594583928 1105 90-91 0.5808106902799217 1105 92-93 0.5314481432339306 1105 94-95 0.43668071538074216 1105 96-97 0.42928509041282226 1105 98-99 0.510240069098046 1105 100-101 0.5241930834054642 1106 1 -0.03419954155944893 1106 2 -0.15795314915210312 1106 3 -0.05334136889135266 1106 4 -0.15203471765426002 1106 5 -0.05683474989850623 1106 6 -0.03692574790709813 1106 7 0.013894516665189371 1106 8 -0.18246881405426763 1106 9 -0.07922912200859145 1106 10-11 -0.060105019494045564 1106 12-13 0.03304515576651568 1106 14-15 0.12243761175493972 1106 16-17 -0.021028134321618097 1106 18-19 -0.0024626668340843594 1106 20-21 0.1774149031236263 1106 22-23 0.2366119622572782 1106 24-25 0.14833904726830838 1106 26-27 -0.03304551720167126 1106 28-29 0.08996158196944748 1106 30-31 0.16240075142101773 1106 32-33 0.26719423195898884 1106 34-35 0.01080999078489242 1106 36-37 0.2741506802798739 1106 38-39 0.025171279770248134 1106 40-41 0.008209006233848015 1106 42-43 0.09080168864107208 1106 44-45 0.13108066113556305 1106 46-47 -0.036477504207795164 1106 48-49 0.054642286168558485 1106 50-51 -0.1136055077140199 1106 52-53 0.036924652138772274 1106 54-55 0.1586971946495197 1106 56-57 0.05650146636903486 1106 58-59 0.17046295310991155 1106 60-61 0.2153891050614405 1106 62-63 0.26095531993789933 1106 64-65 0.34664318612597356 1106 66-67 0.22774052507057263 1106 68-69 0.2302760883111965 1106 70-71 0.2989637927459121 1106 72-73 0.15124400640432256 1106 74-75 0.3246463324857167 1106 76-77 0.2262495315586861 1106 78-79 0.3346435158985628 1106 80-81 0.28103230209764973 1106 82-83 0.2864398391565004 1106 84-85 0.2652687811261991 1106 86-87 0.3740630032938732 1106 88-89 0.6445331255078486 1106 90-91 0.39549932509790153 1106 92-93 0.5028905089163942 1106 94-95 0.5029598597520462 1106 96-97 0.4945194002510114 1106 98-99 0.5111719832272108 1106 100-101 0.47565751989415617 1107 1 0.030416279776336097 1107 2 0.13206826438841546 1107 3 0.13351429644031043 1107 4 0.1330628157774001 1107 5 0.08676371227604562 1107 6 0.03175124086776293 1107 7 0.07700275069606022 1107 8 0.07303792164587009 1107 9 0.20064029859160115 1107 10-11 0.0720455294207838 1107 12-13 0.05655469312296191 1107 14-15 0.05048287022933806 1107 16-17 0.18668999683785614 1107 18-19 0.2058710264771122 1107 20-21 0.2508432911838838 1107 22-23 0.004750526994669713 1107 24-25 0.207423140154944 1107 26-27 0.21649566529476516 1107 28-29 0.1104951308780997 1107 30-31 0.09655812551618226 1107 32-33 0.08050672771639 1107 34-35 0.2519690372343817 1107 36-37 0.2790014334564219 1107 38-39 0.08246484274888388 1107 40-41 0.14785830551121393 1107 42-43 0.11325875356668291 1107 44-45 0.25743258372592237 1107 46-47 0.12979586092701112 1107 48-49 0.16129868832349104 1107 50-51 0.019255328956816697 1107 52-53 0.1725204977884971 1107 54-55 0.2796867840896269 1107 56-57 0.25597137324790253 1107 58-59 0.32843965122835783 1107 60-61 0.37928507351504237 1107 62-63 0.3507450105832035 1107 64-65 0.3893332698671088 1107 66-67 0.4232427559048304 1107 68-69 0.49189586528947515 1107 70-71 0.3514303610871998 1107 72-73 0.28689230597173676 1107 74-75 0.2684888596537611 1107 76-77 0.2283890342955459 1107 78-79 0.006292173386825084 1107 80-81 0.025857568841253453 1107 82-83 0.20813848214279318 1107 84-85 0.2530244939749444 1107 86-87 0.39853985260302593 1107 88-89 0.36490561003394006 1107 90-91 0.43393076782747997 1107 92-93 0.2544912790633269 1107 94-95 0.43170676651366335 1107 96-97 0.41923722902908267 1107 98-99 0.3739593227027953 1107 100-101 0.47928517113113855 1108 1 -0.007200829708295942 1108 2 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68-69 0.01082572946178928 2316 70-71 -0.017947483589004776 2316 72-73 0.4191612376278293 2316 74-75 0.17484122319890005 2316 76-77 0.057884070671562426 2316 78-79 0.1210558188729749 2316 80-81 0.2818551318273066 2316 82-83 0.32696799868223536 2316 84-85 0.41480100546116105 2316 86-87 0.47521261180454744 2316 88-89 0.40472570106357963 2316 90-91 0.41114827132172493 2316 92-93 0.4391219639359214 2316 94-95 0.49514259778628755 2316 96-97 0.41185271840298654 2316 98-99 0.2982291144814546 2316 100-101 0.42319263627825876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32447.0 3 4932.0 4 3851.0 5 3435.0 6 3454.0 7 3722.0 8 3773.0 9 3930.0 10 4025.0 11 4366.0 12 4795.0 13 5422.0 14 5543.0 15 6282.0 16 8028.0 17 10521.0 18 9052.0 19 6358.0 20 6066.0 21 6294.0 22 6707.0 23 7453.0 24 8760.0 25 9822.0 26 11299.0 27 13408.0 28 16105.0 29 19563.0 30 24148.0 31 30782.0 32 39563.0 33 53171.0 34 74414.0 35 111841.0 36 174767.0 37 272568.0 38 207418.0 39 246.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.861749760121874 21.88665276235362 11.851908395693439 24.39968908183107 2 15.328048356064535 20.33389258439657 41.65178248964127 22.68627656989763 3 17.973440715207936 28.700164405239626 29.118195301605226 24.20819957794721 4 11.85935397978037 16.878392476202905 40.911877904099164 30.35037563991756 5 12.42553233402883 38.17981242930804 35.667488020329735 13.727167216333392 6 27.868616985039367 39.43476772732533 19.16720497139119 13.529410316244109 7 25.481334711174547 32.0888986654694 25.273673574751033 17.15609304860502 8 25.75088379102231 34.85251544941399 20.930765120480395 18.465835639083302 9 26.89146053084096 14.248344661672402 22.800454063797112 36.059740743689524 10-11 25.176255539855564 26.137078567761375 28.554813328567246 20.131852563815812 12-13 25.19087603522855 24.35005294132132 28.751651851304654 21.707419172145478 14-15 22.60363728295054 25.363467685901536 26.514416200614054 25.51847883053387 16-17 21.058723001873954 27.499981531324106 27.948277857535096 23.493017609266843 18-19 20.47585120590068 28.086819267159758 30.03206925053825 21.405260276401318 20-21 21.843940603990212 27.064525157777318 28.903147010131075 22.188387228101394 22-23 22.267593946144355 27.290366903575464 28.225129295733257 22.21690985454692 24-25 21.622654270473298 27.24255559449772 29.584037507048578 21.550752627980412 26-27 21.747866548581626 27.211283304783347 28.725157613160956 22.31569253347407 28-29 21.15476007751588 27.996168528913735 28.70919315030152 22.139878243268864 30-31 22.15645830238252 27.591393471889003 28.403364931205065 21.84878329452341 32-33 22.584297699065363 27.93173612097205 27.52170797591131 21.96225820405128 34-35 22.358989470020873 27.17742551080125 29.04313359834068 21.420451420837196 36-37 22.117593658866106 27.120339218159927 28.852011481280538 21.910055641693432 38-39 22.43462572978936 27.043718004384687 29.241724949952026 21.279931315873927 40-41 21.93698169254023 26.372361805539313 29.42404285871996 22.2666136432005 42-43 21.997962786796034 26.779504092073502 28.53214766758787 22.690385453542593 44-45 21.728474304433227 28.228926155050836 28.413072723735823 21.629526816780114 46-47 22.16823169854858 27.060726322453316 28.289707484218823 22.48133449477928 48-49 22.778664489068706 26.939155108488094 28.03764604959809 22.244534352845104 50-51 22.223599884990332 27.080575147201664 27.551282695576162 23.14454227223184 52-53 21.438704450507924 27.986271548991486 27.48205520032802 23.09296880017257 54-55 21.729532098747136 27.95181853657573 27.36284616739856 22.955803197278577 56-57 22.788943058918036 27.02412301548599 27.350912735498678 22.8360211900973 58-59 22.10092876122783 27.10415714167549 28.311682539708666 22.483231557388013 60-61 22.15167895356597 28.433467654007277 27.16545800003456 22.249395392392188 62-63 22.347130164040582 27.37449083157422 26.798026395093714 23.480352609291476 64-65 21.36161379131033 27.739444812872417 27.291969385555394 23.606972010261863 66-67 22.147067655564193 26.776621870102304 27.342523005030873 23.73378746930263 68-69 21.495040359032107 27.26848359769123 27.45549319617377 23.780982847102894 70-71 21.91298889655791 26.752738840822694 27.805537982623775 23.52873427999562 72-73 22.756500253290188 26.604153959080712 28.22980139981598 22.409544387813124 74-75 22.67922526875645 27.324569036980456 27.47539269002254 22.52081300424055 76-77 22.419334131174896 27.054424821321604 27.61903739686233 22.90720365064117 78-79 21.80126962352233 27.199125203949592 27.778768162990055 23.220837009538027 80-81 21.577104827701433 27.24394309951823 28.46703102925641 22.711921043523922 82-83 22.46913996779966 26.102725919793745 29.428297870852187 21.999836241554405 84-85 23.108972644727043 26.973887213497843 27.492069891920135 22.425070249854976 86-87 21.620841220443392 27.69085161045121 28.123891999858504 22.564415169246892 88-89 22.56615946058897 26.695901766117007 27.788659929513273 22.94927884378075 90-91 22.363323793550713 26.28759297585682 28.550360302656703 22.798722927935767 92-93 22.90800054607868 26.666732459278485 27.854124621075677 22.57114237356716 94-95 21.24243809473696 26.956599309048634 28.612284529891625 23.18867806632278 96-97 21.81216810004574 26.92146008653284 27.944091025084166 23.322280788337256 98-99 21.265586511457027 27.689847017737655 27.739367145100957 23.305199325704358 100-101 21.953779397448116 26.911787005679667 27.311184231884628 23.823249364987586 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 65.0 1 42.0 2 32.5 3 41.5 4 31.0 5 34.5 6 65.0 7 117.5 8 176.5 9 204.0 10 198.0 11 207.5 12 273.0 13 377.5 14 468.0 15 580.0 16 714.5 17 888.0 18 1070.0 19 1249.0 20 1492.5 21 1634.5 22 1793.0 23 2061.0 24 2369.0 25 2840.0 26 3762.0 27 4730.0 28 6263.0 29 10465.5 30 16299.0 31 20946.0 32 26498.0 33 31427.0 34 35080.5 35 39688.5 36 42261.0 37 47342.5 38 47771.5 39 41036.0 40 35997.5 41 31530.0 42 29689.5 43 30860.5 44 33414.5 45 38589.0 46 44029.0 47 47215.0 48 49021.5 49 47970.5 50 45677.0 51 46066.0 52 45614.5 53 43741.0 54 41952.0 55 37107.0 56 32708.0 57 27153.0 58 21619.0 59 20384.0 60 17562.0 61 13180.5 62 9954.0 63 8282.5 64 6720.0 65 4308.0 66 2945.0 67 2288.5 68 1847.0 69 1502.5 70 1226.5 71 1001.0 72 825.5 73 651.0 74 537.0 75 455.5 76 373.0 77 304.5 78 256.0 79 218.5 80 187.5 81 147.5 82 124.0 83 113.5 84 91.0 85 67.0 86 47.0 87 39.0 88 39.5 89 32.5 90 21.5 91 20.0 92 20.5 93 13.5 94 7.0 95 6.0 96 5.5 97 3.5 98 2.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.283180022506199E-4 2 0.002872782519692924 3 0.0 4 8.207950056265498E-4 5 0.0013953515095651344 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.103975028132749E-5 12-13 8.207950056265498E-5 14-15 0.0025855042677236316 16-17 0.0042681340292580585 18-19 0.0024623850168796492 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.0519875140663746E-4 42-43 0.0 44-45 6.566360045012398E-4 46-47 0.6849534321953558 48-49 0.49132789036805274 50-51 0.3709993425432005 52-53 0.3096859556228972 54-55 0.30147800556663173 56-57 0.2732836971233597 58-59 0.2729553791211091 60-61 0.25280486173297734 62-63 0.24554082593318236 64-65 0.24270908316377077 66-67 0.23277746359568952 68-69 0.21488413247303073 70-71 0.226703580554053 72-73 0.7587018634509013 74-75 0.491492049369178 76-77 0.5865811507710138 78-79 0.31818118393113204 80-81 0.2735299356250477 82-83 0.25662155850914076 84-85 0.24837256870259397 86-87 0.22489783154167464 88-89 0.2284272500658688 90-91 0.23757911437860482 92-93 0.19608792684418275 94-95 0.1671959426461282 96-97 0.12865961713196167 98-99 0.13575949393063133 100-101 0.1331739896629077 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1218331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.1849764646058 #Duplication Level Percentage of deduplicated Percentage of total 1 86.20965867105816 32.05704128704997 2 7.37494587273181 5.48474377410548 3 2.0467549222790673 2.2832560084128954 4 0.916782325295883 1.3636211675717593 5 0.5266674411309851 0.979205820156492 6 0.34449411907731214 0.7686003426050976 7 0.270029351748856 0.702872456267378 8 0.19796953488743435 0.5889193996398563 9 0.16297869948303506 0.5454323194057848 >10 1.3572345020350325 10.758342449798272 >50 0.26118968441398766 6.859066718366015 >100 0.29347683596263635 22.49642384768988 >500 0.024769710341075144 6.314531714328924 >1k 0.012606013298582887 7.894727157270556 >5k 4.423162560906276E-4 0.903215537331665 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 5881 0.4827095428089739 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 5102 0.4187696118706657 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 4886 0.4010404397491322 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 4216 0.3460471743721534 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 4170 0.34227151734627126 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 3186 0.26150528879261875 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 3088 0.25346149773747856 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 3028 0.24853672770371926 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2791 0.22908388607037006 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 2315 0.19001404380254627 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 2261 0.1855817507721629 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2102 0.17253111018270076 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 2041 0.1675242606483788 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 1999 0.1640769216247473 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 1948 0.15989086709605188 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 1905 0.15636144857185774 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1888 0.1549660970622926 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 1847 0.15160083753922374 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 1812 0.14872805501953082 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 1809 0.14848181651784287 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 1701 0.1396172304570761 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 1631 0.13387166541769027 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1601 0.13140928040081062 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 1601 0.13140928040081062 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 1567 0.12861857738168034 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 1555 0.12763362337492848 No Hit ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA 1503 0.12336548934567043 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 1487 0.12205221733666795 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 1465 0.12024646832428953 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 1444 0.1185227988124738 No Hit GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT 1402 0.11507545978884227 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 1356 0.11129980276296016 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 1354 0.11113564376183484 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 1316 0.10801662274045394 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 1315 0.1079345432398913 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 1297 0.10645711222976349 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 1247 0.10235313720163075 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.207950056265498E-5 7 0.0 0.0 0.0 0.0 8.207950056265498E-5 8 0.0 0.0 0.0 0.0 8.207950056265498E-5 9 0.0 0.0 0.0 0.0 8.207950056265498E-5 10-11 0.0 0.0 0.0 0.0 8.207950056265498E-5 12-13 0.0 8.207950056265498E-5 0.0 0.0 8.207950056265498E-5 14-15 0.0 8.207950056265498E-5 0.0 0.0 1.2311925084398245E-4 16-17 4.103975028132749E-5 8.207950056265498E-5 0.0 0.0 1.6415900112530995E-4 18-19 8.207950056265498E-5 8.207950056265498E-5 0.0 0.0 1.6415900112530995E-4 20-21 8.207950056265498E-5 8.207950056265498E-5 0.0 0.0 1.6415900112530995E-4 22-23 8.207950056265498E-5 8.207950056265498E-5 0.0 0.0 1.6415900112530995E-4 24-25 8.207950056265498E-5 8.207950056265498E-5 0.0 4.103975028132749E-5 1.6415900112530995E-4 26-27 8.207950056265498E-5 8.207950056265498E-5 0.0 5.335167536572574E-4 1.6415900112530995E-4 28-29 8.207950056265498E-5 8.207950056265498E-5 0.0 0.001846788762659737 1.6415900112530995E-4 30-31 8.207950056265498E-5 8.207950056265498E-5 0.0 0.008084830805421515 1.6415900112530995E-4 32-33 8.207950056265498E-5 8.207950056265498E-5 0.0 0.026224400429768265 1.6415900112530995E-4 34-35 8.207950056265498E-5 8.207950056265498E-5 0.0 0.05376207286853901 1.6415900112530995E-4 36-37 8.207950056265498E-5 8.207950056265498E-5 0.0 0.0927087958855188 1.6415900112530995E-4 38-39 8.207950056265498E-5 8.207950056265498E-5 0.0 0.14848181651784287 1.6415900112530995E-4 40-41 8.207950056265498E-5 8.207950056265498E-5 0.0 0.20967208418730213 1.6415900112530995E-4 42-43 8.207950056265498E-5 8.207950056265498E-5 0.0 0.27336577662392236 1.6415900112530995E-4 44-45 1.2311925084398245E-4 8.207950056265498E-5 0.0 0.34859163888959566 1.6415900112530995E-4 46-47 1.6415900112530995E-4 8.207950056265498E-5 0.0 0.4386738907571095 1.6415900112530995E-4 48-49 1.6415900112530995E-4 8.207950056265498E-5 0.0 0.5354045821701984 1.6415900112530995E-4 50-51 1.6415900112530995E-4 8.207950056265498E-5 0.0 0.6377577193718291 1.6415900112530995E-4 52-53 1.6415900112530995E-4 8.207950056265498E-5 0.0 0.7425732415903396 1.6415900112530995E-4 54-55 1.6415900112530995E-4 8.207950056265498E-5 0.0 0.8600290068954988 1.6415900112530995E-4 56-57 1.6415900112530995E-4 8.207950056265498E-5 0.0 0.9938185928126264 1.6415900112530995E-4 58-59 1.6415900112530995E-4 8.207950056265498E-5 0.0 1.1537094599086783 1.6415900112530995E-4 60-61 1.6415900112530995E-4 8.207950056265498E-5 0.0 1.3193869317943974 1.6415900112530995E-4 62-63 1.6415900112530995E-4 8.207950056265498E-5 0.0 1.4948318642470724 1.6415900112530995E-4 64-65 1.6415900112530995E-4 8.207950056265498E-5 0.0 1.6777870710012306 1.6415900112530995E-4 66-67 1.6415900112530995E-4 8.207950056265498E-5 0.0 1.874736832601321 1.6415900112530995E-4 68-69 1.6415900112530995E-4 8.207950056265498E-5 0.0 2.0984855511351186 1.6415900112530995E-4 70-71 1.6415900112530995E-4 8.207950056265498E-5 0.0 2.350469617862469 1.6415900112530995E-4 72-73 1.6415900112530995E-4 8.207950056265498E-5 0.0 2.6099229191410216 1.6415900112530995E-4 74-75 1.6415900112530995E-4 8.207950056265498E-5 0.0 2.8672011136546636 1.6415900112530995E-4 76-77 1.6415900112530995E-4 8.207950056265498E-5 0.0 3.1591578971560272 1.6415900112530995E-4 78-79 1.6415900112530995E-4 8.207950056265498E-5 0.0 3.478939631348131 1.6415900112530995E-4 80-81 1.6415900112530995E-4 8.207950056265498E-5 0.0 3.8395969568204373 1.6415900112530995E-4 82-83 1.6415900112530995E-4 8.207950056265498E-5 0.0 4.2117043726212335 1.6415900112530995E-4 84-85 1.6415900112530995E-4 8.207950056265498E-5 0.0 4.573716009852824 1.6415900112530995E-4 86-87 1.6415900112530995E-4 8.207950056265498E-5 0.0 4.961828928263337 1.6415900112530995E-4 88-89 1.6415900112530995E-4 8.207950056265498E-5 0.0 5.386426184673951 1.6415900112530995E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1205 0.0 50.705856 2 ACCTGGG 1295 0.0 47.179966 3 GTACCTG 1455 0.0 42.970108 1 CCTGGGG 1325 0.0 40.392452 4 TATTCTC 825 0.0 39.036854 5 CTGGGGG 1270 0.0 38.410797 5 TCTCTGC 985 0.0 32.213696 8 GTATCAA 4165 0.0 32.183025 1 GTACTAG 975 0.0 31.090721 1 ATAACGC 110 2.7959486E-6 30.139982 3 TGGGGGG 3865 0.0 29.40788 6 TTCTCTG 1100 0.0 29.276438 7 TAGTACT 1310 0.0 27.83922 4 ATCAACG 4580 0.0 27.50767 3 CAACGCA 4565 0.0 27.493176 5 CTGTGCG 1335 0.0 27.317888 9 TAGGCAT 1180 0.0 27.292715 5 TATCAAC 4880 0.0 27.273567 2 GGTATCA 2765 0.0 26.722982 1 AGGGCTA 1955 0.0 26.64807 5 >>END_MODULE