##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931076_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1218331 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56693624310635 33.0 33.0 33.0 33.0 33.0 2 32.54980214736389 33.0 33.0 33.0 33.0 33.0 3 32.591990189858095 33.0 33.0 33.0 33.0 33.0 4 36.53619911173565 37.0 37.0 37.0 37.0 37.0 5 36.463676948218506 37.0 37.0 37.0 37.0 37.0 6 36.465185569438844 37.0 37.0 37.0 37.0 37.0 7 36.39486067415177 37.0 37.0 37.0 37.0 37.0 8 36.41043279699851 37.0 37.0 37.0 37.0 37.0 9 36.396659856804106 37.0 37.0 37.0 37.0 37.0 10-11 36.35882038624971 37.0 37.0 37.0 37.0 37.0 12-13 36.26849312707302 37.0 37.0 37.0 33.0 37.0 14-15 38.09250646991663 40.0 37.0 40.0 33.0 40.0 16-17 38.08060494233504 40.0 37.0 40.0 33.0 40.0 18-19 38.01756952749294 40.0 37.0 40.0 33.0 40.0 20-21 37.92184102678172 40.0 37.0 40.0 33.0 40.0 22-23 37.772254009788796 40.0 37.0 40.0 33.0 40.0 24-25 37.937995914082464 40.0 37.0 40.0 33.0 40.0 26-27 37.85501271821861 40.0 37.0 40.0 33.0 40.0 28-29 37.76337834299546 40.0 37.0 40.0 33.0 40.0 30-31 37.60575287011494 40.0 37.0 40.0 33.0 40.0 32-33 37.45895778733366 40.0 37.0 40.0 33.0 40.0 34-35 37.352963193089565 40.0 37.0 40.0 33.0 40.0 36-37 37.27363007261573 40.0 37.0 40.0 33.0 40.0 38-39 37.08483490939655 40.0 37.0 40.0 33.0 40.0 40-41 36.85883105658479 38.5 37.0 40.0 33.0 40.0 42-43 36.629131163862695 37.0 37.0 40.0 33.0 40.0 44-45 36.333708573450075 37.0 37.0 40.0 33.0 40.0 46-47 36.0162365564038 37.0 37.0 40.0 33.0 40.0 48-49 35.68670541913487 37.0 37.0 40.0 30.0 40.0 50-51 35.392737277472214 37.0 37.0 40.0 27.0 40.0 52-53 35.12614429083722 37.0 35.0 40.0 27.0 40.0 54-55 34.85023158731084 37.0 33.0 40.0 27.0 40.0 56-57 34.494249510190585 37.0 33.0 40.0 27.0 40.0 58-59 34.15378086907417 37.0 33.0 40.0 27.0 40.0 60-61 33.886475021976786 37.0 33.0 38.5 27.0 40.0 62-63 33.531669144099595 37.0 33.0 37.0 24.5 40.0 64-65 33.13859082630254 37.0 33.0 37.0 22.0 40.0 66-67 32.75455890066 37.0 33.0 37.0 22.0 40.0 68-69 32.410915834859324 37.0 33.0 37.0 22.0 40.0 70-71 32.124047980392845 37.0 33.0 37.0 22.0 40.0 72-73 31.77675278721464 35.0 33.0 37.0 18.5 40.0 74-75 31.351981522262832 33.0 33.0 37.0 15.0 37.0 76-77 29.796455971324704 33.0 27.0 37.0 15.0 37.0 78-79 30.58822315117977 33.0 30.0 37.0 15.0 37.0 80-81 30.64725185520191 33.0 33.0 37.0 15.0 37.0 82-83 30.402146461019214 33.0 30.0 37.0 15.0 37.0 84-85 30.075069911214605 33.0 27.0 37.0 10.5 37.0 86-87 29.699357563749096 33.0 27.0 37.0 6.0 37.0 88-89 29.326640707656622 33.0 27.0 37.0 4.0 37.0 90-91 28.864105895688446 33.0 27.0 37.0 2.0 37.0 92-93 28.394898020324526 33.0 27.0 37.0 2.0 37.0 94-95 27.845246899241666 33.0 27.0 37.0 2.0 37.0 96-97 27.28615047963156 33.0 27.0 33.0 2.0 37.0 98-99 26.53170813186236 33.0 24.5 33.0 2.0 37.0 100-101 24.84235605923185 33.0 18.5 33.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.040785920616762894 1101 2 0.1658098542750821 1101 3 0.158714778205983 1101 4 0.252263388608867 1101 5 0.15126445282724177 1101 6 0.1705682671165505 1101 7 0.03181701789541336 1101 8 0.16730303882930286 1101 9 0.10186736517694328 1101 10-11 0.16950173524816847 1101 12-13 0.18104492744454603 1101 14-15 0.23452522232023654 1101 16-17 0.12802378314266605 1101 18-19 0.168340962144498 1101 20-21 -0.0508762668086149 1101 22-23 0.04776517194712682 1101 24-25 0.21740550537889902 1101 26-27 0.1659523586713405 1101 28-29 0.23527772695366167 1101 30-31 0.20291168373632473 1101 32-33 9.411655541029518E-4 1101 34-35 0.12987235255535268 1101 36-37 0.05951928890407032 1101 38-39 -0.05431318512016503 1101 40-41 0.010099401500674787 1101 42-43 0.03836429459767743 1101 44-45 0.09942373225310774 1101 46-47 0.06721706741699052 1101 48-49 -0.01298143767687776 1101 50-51 -0.1549480774357832 1101 52-53 -0.18809037830929753 1101 54-55 -0.3195245236990516 1101 56-57 -0.19861198890168907 1101 58-59 -0.41201551010129833 1101 60-61 -0.3259237095705956 1101 62-63 -0.5462204331740494 1101 64-65 -0.6957883778638703 1101 66-67 -0.6989566078947504 1101 68-69 -0.7303472553336228 1101 70-71 -0.6176370229948027 1101 72-73 -0.7643356856289572 1101 74-75 -0.9082397743118413 1101 76-77 -1.2063783331752695 1101 78-79 -0.976927657563504 1101 80-81 -1.296038486208957 1101 82-83 -1.4495505231132633 1101 84-85 -1.3162771666833741 1101 86-87 -1.7275193610355295 1101 88-89 -1.9853973468083481 1101 90-91 -1.8112229314921677 1101 92-93 -1.8800476985613805 1101 94-95 -2.521186332007076 1101 96-97 -2.5096340298936433 1101 98-99 -2.8892436391935057 1101 100-101 -4.030731498396129 1102 1 0.048215726366038325 1102 2 0.21889968604416055 1102 3 0.15540619283326151 1102 4 0.19359191527065178 1102 5 0.15117158025537236 1102 6 0.17671023986928702 1102 7 0.19744673649925915 1102 8 0.24690489411371885 1102 9 0.19266887869029148 1102 10-11 0.22996177953290697 1102 12-13 0.18390965931755687 1102 14-15 0.24176928292578026 1102 16-17 0.18545308583217945 1102 18-19 0.14502336813230698 1102 20-21 0.18819862950302735 1102 22-23 0.16792331845825004 1102 24-25 0.18918849623172207 1102 26-27 0.12532563907762295 1102 28-29 0.19055842269305856 1102 30-31 0.19436005471272466 1102 32-33 0.16831998426142292 1102 34-35 0.17517327129726823 1102 36-37 0.1716296400939541 1102 38-39 0.26739159928776957 1102 40-41 0.27217419023492084 1102 42-43 0.20077868736971283 1102 44-45 -0.06860674233346487 1102 46-47 -0.06346625026989017 1102 48-49 0.02474262707696795 1102 50-51 0.10790567999499245 1102 52-53 0.3087279621661878 1102 54-55 0.30750580616111023 1102 56-57 0.2685374345531244 1102 58-59 0.33999498759507674 1102 60-61 0.057561844487821645 1102 62-63 -0.1919332417721975 1102 64-65 -0.11096592313344189 1102 66-67 -0.04345812597433252 1102 68-69 -0.12794286184182369 1102 70-71 -0.17473275836369595 1102 72-73 -0.2702102627685292 1102 74-75 -0.36328674074537304 1102 76-77 -0.26686740013871457 1102 78-79 -0.4113394994355666 1102 80-81 -0.39431585478846287 1102 82-83 -0.4489600083438141 1102 84-85 -0.26811964241847974 1102 86-87 -0.48456045637464484 1102 88-89 -0.772372432967007 1102 90-91 -0.7892988130270133 1102 92-93 -0.5764000509075373 1102 94-95 -1.028293018600074 1102 96-97 -1.106159202617281 1102 98-99 -1.408851747897753 1102 100-101 -2.2000308444958314 1103 1 0.11866600081621215 1103 2 0.28644987797518695 1103 3 0.10433528484103505 1103 4 0.1251111267831888 1103 5 0.23657348750904816 1103 6 0.1163709078340247 1103 7 0.22877802381968593 1103 8 0.09106065388552054 1103 9 0.09295715425660944 1103 10-11 0.15976852011305454 1103 12-13 0.2103114561125281 1103 14-15 0.31761615106047003 1103 16-17 0.22521461053774772 1103 18-19 0.21133216302781221 1103 20-21 0.13496511329916672 1103 22-23 0.14786712168870508 1103 24-25 0.33535243861125963 1103 26-27 0.18471632574814834 1103 28-29 0.20778492729586873 1103 30-31 0.2488710655687214 1103 32-33 0.28646279076448167 1103 34-35 0.3956928892689717 1103 36-37 0.4763433390672702 1103 38-39 0.4355371853073038 1103 40-41 0.3337679144476908 1103 42-43 0.3206906380632617 1103 44-45 0.6191622300323658 1103 46-47 0.5586790599638505 1103 48-49 0.4903423203916262 1103 50-51 0.5736883882258965 1103 52-53 0.6335523653101518 1103 54-55 0.46557690835850707 1103 56-57 0.5332484767418535 1103 58-59 0.6032440763107445 1103 60-61 0.39191998915696047 1103 62-63 0.2914221472048908 1103 64-65 0.3851694660313498 1103 66-67 0.4752599321471678 1103 68-69 0.5692992080532733 1103 70-71 0.47304005478854094 1103 72-73 0.4141734751107933 1103 74-75 0.15926055254703897 1103 76-77 0.30340816184702746 1103 78-79 0.39651938487373783 1103 80-81 0.23981169830571503 1103 82-83 0.16316893523055498 1103 84-85 0.15528157609266557 1103 86-87 0.1564646293030485 1103 88-89 -0.1343698007288019 1103 90-91 -0.47007748193462007 1103 92-93 -0.1815820081636268 1103 94-95 -0.17824901560541306 1103 96-97 -0.0449098496670608 1103 98-99 -0.3266643334039827 1103 100-101 -0.9311321178031591 1104 1 0.030045876024061613 1104 2 0.1854493182214796 1104 3 0.09954856746726648 1104 4 0.12023180309737569 1104 5 0.028900745868305933 1104 6 0.0027573683796191517 1104 7 0.07461320927926351 1104 8 0.0871464937630364 1104 9 -0.015260524082570726 1104 10-11 0.07582398512021626 1104 12-13 0.27267357560347705 1104 14-15 0.24294665269857063 1104 16-17 0.13263196454904858 1104 18-19 0.20355220385690842 1104 20-21 0.03761015536301926 1104 22-23 0.048653863757486704 1104 24-25 0.10794014707835942 1104 26-27 0.2745084348671938 1104 28-29 0.36278422760373275 1104 30-31 0.20980354939348445 1104 32-33 0.15176690989324015 1104 34-35 0.19291284778134354 1104 36-37 0.18116380629697915 1104 38-39 0.32072799546848074 1104 40-41 0.2477911853849406 1104 42-43 0.35436538000033835 1104 44-45 0.21934270944495182 1104 46-47 0.08124310237342769 1104 48-49 0.19058597788821174 1104 50-51 0.2708544292854782 1104 52-53 0.23892805882853452 1104 54-55 0.22316651011021094 1104 56-57 0.3746159221247112 1104 58-59 0.16365014469947425 1104 60-61 0.04688121798098166 1104 62-63 0.051757011629256056 1104 64-65 0.2496267518843993 1104 66-67 0.21041925616223978 1104 68-69 0.044160710168810624 1104 70-71 0.08591274374657587 1104 72-73 0.23590735338082425 1104 74-75 0.07914683199584616 1104 76-77 0.07342758859337906 1104 78-79 0.23623740894314693 1104 80-81 0.1996092403284031 1104 82-83 0.2558071450064894 1104 84-85 0.36614511966290664 1104 86-87 0.37678739316930177 1104 88-89 0.23398929773846078 1104 90-91 0.2290178572925896 1104 92-93 0.28702856788879316 1104 94-95 0.3061066914129107 1104 96-97 0.307599164013979 1104 98-99 0.5134025077741349 1104 100-101 -0.19623003354376323 1105 1 0.1750272018877439 1105 2 0.2604493182214753 1105 3 0.11957657867174731 1105 4 0.07515710655209062 1105 5 0.09729477014468557 1105 6 0.10299079508363462 1105 7 0.08411367613266663 1105 8 0.14970485043903636 1105 9 0.14897850486234177 1105 10-11 0.08392389174953507 1105 12-13 0.1894686269573569 1105 14-15 0.24733507473404615 1105 16-17 0.2961823847171132 1105 18-19 0.20440421132656184 1105 20-21 0.12307934303808565 1105 22-23 0.21481866301051866 1105 24-25 0.11193174371701531 1105 26-27 0.11424933122574288 1105 28-29 0.30028422760373275 1105 30-31 0.1832629331469846 1105 32-33 0.23884674182601628 1105 34-35 0.2174343230381126 1105 36-37 0.05387622459763719 1105 38-39 0.19418737922197948 1105 40-41 0.20163105466599518 1105 42-43 0.07909693929071437 1105 44-45 0.0698679195289813 1105 46-47 -7.10899701772405E-5 1105 48-49 0.13015180421873396 1105 50-51 0.1822106384358051 1105 52-53 0.2757627927220909 1105 54-55 0.3049825698674411 1105 56-57 0.5250360901919393 1105 58-59 0.3934353921317779 1105 60-61 0.2955973711088973 1105 62-63 0.44928268856669007 1105 64-65 0.5540151739198791 1105 66-67 0.5253468938840058 1105 68-69 0.564667246116521 1105 70-71 0.4136671788539559 1105 72-73 0.3992710321856805 1105 74-75 0.2870483259267509 1105 76-77 0.2652109686120525 1105 78-79 0.4860389962447371 1105 80-81 0.45057796115006354 1105 82-83 0.3256834288533632 1105 84-85 0.2836171084584258 1105 86-87 0.19062959671738966 1105 88-89 0.5135411184667511 1105 90-91 0.3763101075260167 1105 92-93 0.5369118545367861 1105 94-95 0.3384596325893803 1105 96-97 0.5435702191026799 1105 98-99 0.33823910908132504 1105 100-101 -0.7717552436277941 1106 1 -0.0012396238940368676 1106 2 0.156694714790234 1106 3 0.08261824373443005 1106 4 0.15855652398546027 1106 5 0.12232332104227339 1106 6 0.13045932493696455 1106 7 0.11976316093332429 1106 8 0.038186327643231266 1106 9 -0.009663755598836588 1106 10-11 0.09546551190069152 1106 12-13 0.13242842472717342 1106 14-15 0.15927384107863674 1106 16-17 0.20873355177515407 1106 18-19 0.23357456715415736 1106 20-21 0.1868508203706014 1106 22-23 0.16773181695439376 1106 24-25 0.16248255148286006 1106 26-27 0.11638519500267108 1106 28-29 0.2952541368824555 1106 30-31 0.26926112385415024 1106 32-33 0.10403421536029356 1106 34-35 0.21118424283896076 1106 36-37 0.21290568655757625 1106 38-39 0.1689778990407973 1106 40-41 0.2783960775708678 1106 42-43 0.28786495201992324 1106 44-45 0.3053056785722461 1106 46-47 0.20619984200811814 1106 48-49 0.19711135818314318 1106 50-51 0.281173182224542 1106 52-53 0.3519221516421567 1106 54-55 0.26813747613068273 1106 56-57 0.28851788819273594 1106 58-59 0.36133680492001474 1106 60-61 0.3248941207977296 1106 62-63 0.32801836499840675 1106 64-65 0.45940584002193674 1106 66-67 0.3737291166195007 1106 68-69 0.2662346583144952 1106 70-71 0.3991732675734596 1106 72-73 0.45996863647665975 1106 74-75 0.4979900564319415 1106 76-77 0.43551354396717556 1106 78-79 0.6839323249585973 1106 80-81 0.5602458083482276 1106 82-83 0.6404086344839861 1106 84-85 0.8149165913343062 1106 86-87 0.9803923355504587 1106 88-89 0.8617863610531415 1106 90-91 0.6270383158128361 1106 92-93 0.6834421184882267 1106 94-95 1.0349295867796258 1106 96-97 0.8265452226314451 1106 98-99 0.8724886375103189 1106 100-101 0.7130688315155993 1107 1 0.07818362985333493 1107 2 0.1203305735225797 1107 3 0.05057136844516208 1107 4 -0.04529606199267988 1107 5 9.782791717043438E-4 1107 6 0.020384470110897723 1107 7 0.08783353557771534 1107 8 -0.01699210824265407 1107 9 0.021697516130970484 1107 10-11 0.029464907137885632 1107 12-13 -0.021751535407851463 1107 14-15 -0.040099797433647666 1107 16-17 -0.003959987753503924 1107 18-19 -0.0387649392853362 1107 20-21 -0.050145544881459614 1107 22-23 0.02247951361029976 1107 24-25 0.035792763952080975 1107 26-27 0.02750511700060798 1107 28-29 0.06304928273520005 1107 30-31 0.0028285456515320107 1107 32-33 -0.012907165843920154 1107 34-35 0.06647406616183105 1107 36-37 0.1331171067011141 1107 38-39 0.11538573133636731 1107 40-41 0.12904835746497412 1107 42-43 0.3025187253079267 1107 44-45 0.3888913049645808 1107 46-47 0.27288419901564964 1107 48-49 0.30119566705649703 1107 50-51 0.11261776311773275 1107 52-53 0.13299955511152461 1107 54-55 0.3584631223938004 1107 56-57 0.24614962466424828 1107 58-59 0.27265204062324244 1107 60-61 0.2112129048542073 1107 62-63 0.09713195549993259 1107 64-65 0.2780306834315738 1107 66-67 0.4094344983914482 1107 68-69 0.24833985001813375 1107 70-71 0.1548370564742143 1107 72-73 0.02590074259401831 1107 74-75 0.18727248683793718 1107 76-77 -0.10490898776744473 1107 78-79 0.13052282030871254 1107 80-81 0.11239487621125477 1107 82-83 0.21935239771316972 1107 84-85 0.40643911257814125 1107 86-87 0.4669048905499338 1107 88-89 0.32449471109973516 1107 90-91 0.7294120098645607 1107 92-93 0.7614423283038967 1107 94-95 0.9252280804265283 1107 96-97 0.8271246841456978 1107 98-99 1.0949821116258533 1107 100-101 1.7740008450672207 1108 1 0.038793501695231214 1108 2 0.19228252442539429 1108 3 0.12178499827147249 1108 4 0.05730087176222298 1108 5 -1.4084788589485697E-4 1108 6 0.07036030581699038 1108 7 0.0058455420292915505 1108 8 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94-95 0.21545493775369806 2316 96-97 0.3266408652014974 2316 98-99 0.8828400480484611 2316 100-101 1.2965378482568468 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 6.0 5 28.0 6 62.0 7 132.0 8 347.0 9 714.0 10 1210.0 11 1784.0 12 2660.0 13 3938.0 14 4804.0 15 6074.0 16 7602.0 17 9807.0 18 9635.0 19 7625.0 20 6816.0 21 6974.0 22 7555.0 23 8568.0 24 9932.0 25 11481.0 26 13784.0 27 16928.0 28 20496.0 29 25507.0 30 31795.0 31 40510.0 32 52575.0 33 71023.0 34 101333.0 35 149342.0 36 220501.0 37 275731.0 38 91045.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.412114541063595 24.850170037293204 14.171745880497461 25.56596954114574 2 13.304594564202995 22.186499399588453 40.50549481216517 24.003411224043383 3 15.576555139777284 29.456280764422804 30.809361331198172 24.157802764601737 4 10.975342497235973 18.513441749409644 39.757996800541065 30.753218952813317 5 11.834140311623031 38.79733832595576 36.20928959371468 13.159231768706533 6 28.21047810488283 40.33197874797572 18.695329922656487 12.762213224484972 7 25.01413819397192 32.540992554568504 25.342784514224785 17.10208473723479 8 25.918982608174623 35.52564943352833 20.420723103984056 18.134644854312988 9 26.521856539807327 14.06243459289799 22.597717697407354 36.817991169887335 10-11 24.915765912547574 26.82111839885877 28.551436350220094 19.71167933837356 12-13 24.74463015387444 24.706340066861962 29.08741548889423 21.461614290369365 14-15 22.242099206534863 25.65862859478613 26.534912219789923 25.564359978889083 16-17 20.907864939823416 27.566728581969922 28.00593598948069 23.519470488725972 18-19 20.490572758962877 28.075826684209794 30.353532824823464 21.080067732003865 20-21 21.522312081035448 27.240052169730554 29.287525311265988 21.950110437968007 22-23 22.00145116556995 27.233362690434703 28.691094620427453 22.074091523567898 24-25 21.389630568375917 27.293855282349377 29.976623758239757 21.33989039103495 26-27 21.48562254428394 27.35098261474099 29.142819151774024 22.020575689201046 28-29 20.89703044574914 28.105005946659816 29.024583631213517 21.97337997637752 30-31 22.020288410949078 27.529054091211663 28.644719702609557 21.805937795229706 32-33 22.60379158044899 27.985785472092562 27.974909938268006 21.43551300919044 34-35 22.049303514397973 27.458917157980878 29.347525426177285 21.14425390144386 36-37 22.018236423435013 27.22540097888012 29.257812532062307 21.49855006562256 38-39 22.12204410955981 26.940689304211503 29.854390846486584 21.082875739742107 40-41 21.511966733809736 26.404217701146028 30.05149003442519 22.032325530619048 42-43 21.764758512734936 26.816212919542004 29.138699935892333 22.280328631830727 44-45 21.42906976839627 28.205471255348506 28.92629342928974 21.43916554696548 46-47 21.90730597842458 27.19371829166294 28.728522872684024 22.170452857228454 48-49 22.721963671583232 26.85245376868336 28.587903113278013 21.837679446455393 50-51 21.983393675446163 27.296933263620478 27.652706858809307 23.066966202124053 52-53 21.064390547396396 28.021941492090406 27.775169473648788 23.138498486864407 54-55 21.557360027775704 28.139930774149224 27.51809647788655 22.78461272018852 56-57 22.819701706679055 27.24050360698365 27.549532926602048 22.390261759735246 58-59 21.93599276387123 27.42600327825525 28.571094390604852 22.066909567268663 60-61 22.053899966429487 28.726963362173336 27.234963240695677 21.98417343070151 62-63 22.416568239665573 27.444963642885227 26.793539686669714 23.344928430779486 64-65 21.238234929588103 27.845922003133794 27.30817815519756 23.60766491208054 66-67 22.021701542435547 26.918728179131502 27.403871212397927 23.655699066035023 68-69 21.562698800323414 27.358372084432936 27.45182619259673 23.627102922646923 70-71 21.74822531602036 26.77327835031238 27.975388969441823 23.50310736422544 72-73 22.47221814104706 26.960858748566686 28.170546427859094 22.396376682527162 74-75 22.190480255365742 27.71742654500296 27.659560497106288 22.432532702525013 76-77 21.907085969158892 27.28059958516271 27.7718132263654 23.040501219312997 78-79 21.288672444772413 27.500313968005635 28.01731461728326 23.19369896993869 80-81 21.02728540899795 27.699801812132346 28.628892089189055 22.644020689680648 82-83 21.948654934615135 26.46873292940536 29.62603624024398 21.95657589573553 84-85 22.630958155935808 27.386689255353115 27.451779474665294 22.53057311404578 86-87 21.08194269682934 27.898380319897697 28.1961657256221 22.82351125765086 88-89 21.77827700354009 26.924005052814053 27.893692272461262 23.4040256711846 90-91 21.45222499149451 26.449678473944466 28.8428304142431 23.255266120317927 92-93 22.047618949904273 26.989023797162893 27.765217195025105 23.19814005790773 94-95 20.223641884055237 27.16231889530135 28.637956409286154 23.97608281135726 96-97 20.839217607965924 27.31103731912653 27.86000371832803 23.989741354579515 98-99 20.168615918005862 27.94868553783824 27.80804231362413 24.07465623053177 100-101 19.120525276618515 28.03646304222298 27.761660536004424 25.08135114515408 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 68.0 1 51.5 2 37.5 3 36.5 4 34.5 5 36.5 6 60.0 7 120.5 8 180.0 9 234.0 10 268.0 11 307.0 12 388.5 13 494.5 14 576.0 15 710.0 16 862.5 17 985.5 18 1131.5 19 1265.0 20 1450.5 21 1631.0 22 1818.0 23 2080.5 24 2366.0 25 2846.5 26 3789.5 27 4717.0 28 6502.5 29 10881.5 30 16379.0 31 20822.0 32 26721.5 33 32043.0 34 35597.0 35 40627.5 36 43667.5 37 48648.5 38 48840.5 39 41163.0 40 35749.5 41 31994.5 42 30608.0 43 31507.5 44 34035.5 45 40087.0 46 45537.0 47 48971.5 48 51126.0 49 48997.5 50 46565.5 51 46595.5 52 45666.0 53 43652.5 54 42107.5 55 37324.5 56 31985.0 57 26429.5 58 21154.5 59 19943.0 60 16745.5 61 11769.5 62 8420.0 63 6397.5 64 4737.5 65 2847.5 66 1768.5 67 1202.0 68 924.5 69 703.0 70 524.5 71 421.0 72 326.5 73 241.0 74 187.0 75 151.0 76 113.0 77 88.5 78 71.5 79 51.5 80 34.0 81 30.5 82 27.0 83 24.5 84 21.0 85 14.5 86 15.0 87 9.5 88 5.0 89 5.5 90 4.0 91 2.5 92 0.5 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07805760503508488 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.283180022506199E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.207950056265498E-5 40-41 0.003036941520818234 42-43 0.005581406038260538 44-45 0.0 46-47 0.0 48-49 8.207950056265498E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002503424767160977 68-69 0.007058837048388327 70-71 0.0012722322587211522 72-73 0.0 74-75 0.0 76-77 0.0018057490123784094 78-79 0.004432293030383369 80-81 0.003488378773912837 82-83 0.0036525377750381465 84-85 0.0018057490123784094 86-87 3.283180022506199E-4 88-89 0.0 90-91 4.103975028132749E-5 92-93 0.0011080732575958422 94-95 0.003611498024756819 96-97 0.0034062992733501817 98-99 0.0 100-101 1.2311925084398248E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1218331.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.35395946210798 #Duplication Level Percentage of deduplicated Percentage of total 1 83.44554511242895 26.997937838610287 2 8.959898552124063 5.797763890800438 3 2.3763600617259106 2.3065397131335756 4 1.0794060043792388 1.3969223243536735 5 0.625545516563408 1.011943714229795 6 0.41574093329451534 0.8070519181529812 7 0.321769389671102 0.7287359652696243 8 0.2418923452832478 0.6260940106790741 9 0.19299017805101032 0.561959675752265 >10 1.6226505565909881 11.166739798370976 >50 0.3144669786770213 7.305684381569195 >100 0.354028380785504 24.226823750887128 >500 0.03448987090720331 7.6967270477778165 >1k 0.014455313541989626 7.913668003103487 >5k 7.608059758941908E-4 1.4554079673096627 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 6662 0.5468136327484074 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 6019 0.4940365138866203 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 5057 0.41507603434534623 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 4899 0.4021074732564467 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 4128 0.3388241783226398 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 3969 0.3257735377331776 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 3299 0.2707802723561988 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2848 0.23376241760244137 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 2750 0.2257186265473012 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 2610 0.21422749646852948 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2384 0.19567752934136948 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 2221 0.1822985707496567 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 2145 0.17606052870689493 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2129 0.17474725669789246 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 2063 0.16933000966075723 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 2059 0.1690016916585066 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1902 0.15611521007016976 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 1870 0.1534886660521648 No Hit ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA 1846 0.15151875803866108 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 1823 0.14963092952572002 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 1718 0.14101258196664126 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 1697 0.1392889124548255 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 1682 0.13805771994638566 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1652 0.135595334929506 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 1649 0.13534909642781806 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 1628 0.13362542691600232 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 1531 0.1256637153614248 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 1524 0.1250891588574862 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 1513 0.12418628435129699 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 1486 0.12197013783610529 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 1470 0.1206568658271028 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 1413 0.11597833429503147 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 1403 0.11515753928940493 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 1396 0.11458298278546634 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 1375 0.1128593132736506 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 1338 0.10982237175283235 No Hit CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT 1299 0.10662127123088881 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 1264 0.1037484887111959 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 1260 0.10342017070894527 No Hit ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT 1260 0.10342017070894527 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 1236 0.10145026269544155 No Hit CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG 1227 0.10071154719037766 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.207950056265498E-5 7 0.0 0.0 0.0 0.0 8.207950056265498E-5 8 0.0 0.0 0.0 0.0 8.207950056265498E-5 9 0.0 0.0 0.0 0.0 8.207950056265498E-5 10-11 0.0 0.0 0.0 0.0 8.207950056265498E-5 12-13 0.0 0.0 0.0 0.0 8.207950056265498E-5 14-15 4.103975028132749E-5 0.0 0.0 0.0 8.207950056265498E-5 16-17 8.207950056265498E-5 0.0 0.0 0.0 8.207950056265498E-5 18-19 8.207950056265498E-5 0.0 0.0 0.0 8.207950056265498E-5 20-21 8.207950056265498E-5 0.0 0.0 0.0 8.207950056265498E-5 22-23 8.207950056265498E-5 0.0 0.0 0.0 8.207950056265498E-5 24-25 8.207950056265498E-5 0.0 0.0 4.103975028132749E-5 8.207950056265498E-5 26-27 8.207950056265498E-5 0.0 0.0 5.335167536572574E-4 8.207950056265498E-5 28-29 8.207950056265498E-5 0.0 0.0 0.001846788762659737 8.207950056265498E-5 30-31 8.207950056265498E-5 0.0 0.0 0.00800275130485886 8.207950056265498E-5 32-33 8.207950056265498E-5 0.0 0.0 0.02630647993033092 8.207950056265498E-5 34-35 8.207950056265498E-5 0.0 0.0 0.05380311261882034 8.207950056265498E-5 36-37 8.207950056265498E-5 0.0 0.0 0.09295503438720676 8.207950056265498E-5 38-39 8.207950056265498E-5 0.0 0.0 0.14876909476981215 8.207950056265498E-5 40-41 8.207950056265498E-5 0.0 0.0 0.20926168668448886 8.207950056265498E-5 42-43 8.207950056265498E-5 0.0 0.0 0.27197042511435726 8.207950056265498E-5 44-45 8.207950056265498E-5 0.0 0.0 0.346498611625248 8.207950056265498E-5 46-47 8.207950056265498E-5 0.0 0.0 0.4362525454905112 8.207950056265498E-5 48-49 8.207950056265498E-5 0.0 0.0 0.5319572431465669 8.207950056265498E-5 50-51 8.207950056265498E-5 0.0 0.0 0.6343924598487602 8.207950056265498E-5 52-53 8.207950056265498E-5 0.0 0.0 0.73949526031924 8.207950056265498E-5 54-55 8.207950056265498E-5 0.0 0.0 0.8548169586097702 8.207950056265498E-5 56-57 8.207950056265498E-5 0.0 0.0 0.9858979210083303 8.207950056265498E-5 58-59 8.207950056265498E-5 0.0 0.0 1.1426697670830013 8.207950056265498E-5 60-61 1.6415900112530995E-4 0.0 0.0 1.305433416698746 8.207950056265498E-5 62-63 1.6415900112530995E-4 0.0 0.0 1.4777182883797588 8.207950056265498E-5 64-65 1.6415900112530995E-4 0.0 0.0 1.6568157586074719 8.207950056265498E-5 66-67 2.462385016879649E-4 0.0 0.0 1.851180015939839 8.207950056265498E-5 68-69 4.1039750281327486E-4 0.0 0.0 2.0699218849393146 8.207950056265498E-5 70-71 4.1039750281327486E-4 0.0 0.0 2.3169811816329062 8.207950056265498E-5 72-73 4.1039750281327486E-4 0.0 0.0 2.571879070630231 8.207950056265498E-5 74-75 4.1039750281327486E-4 0.0 0.0 2.82410937585927 8.207950056265498E-5 76-77 4.1039750281327486E-4 0.0 0.0 3.1097870775675904 8.207950056265498E-5 78-79 5.745565039385848E-4 0.0 0.0 3.420006549944145 8.207950056265498E-5 80-81 5.745565039385848E-4 0.0 0.0 3.771183693101464 8.207950056265498E-5 82-83 5.745565039385848E-4 0.0 0.0 4.133236370083335 8.207950056265498E-5 84-85 5.745565039385848E-4 0.0 0.0 4.481869048723213 8.207950056265498E-5 86-87 5.745565039385848E-4 0.0 0.0 4.854797259529635 8.207950056265498E-5 88-89 5.745565039385848E-4 0.0 0.0 5.261583264318153 8.207950056265498E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 975 0.0 47.741238 2 ACCTGGG 1025 0.0 44.94901 3 CCTGGGG 990 0.0 42.220142 4 GTACCTG 1125 0.0 41.835712 1 CTGGGGG 1005 0.0 41.11738 5 TGGGGGG 3375 0.0 30.398504 6 AGGGCTA 2055 0.0 30.04718 5 GTATCAA 4690 0.0 28.7879 1 CTATTGA 2120 0.0 28.677832 9 TATTCTC 930 0.0 28.60074 5 GCTATTG 2130 0.0 28.320202 8 TATTCGC 185 2.910383E-10 28.241852 9 TAGCCCC 205 3.092282E-11 27.803509 9 CTGTGCG 1440 0.0 27.377123 9 GTACTAG 820 0.0 27.24887 1 CGCGCGA 35 0.008386998 27.14709 78-79 ATTTAGG 2350 0.0 27.10825 1 CATGGGG 3385 0.0 27.081385 4 ACTATAT 2440 0.0 27.058094 3 TCTAACG 515 0.0 26.746256 2 >>END_MODULE