##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931075_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4108935 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36103783583824 33.0 33.0 33.0 27.0 33.0 2 31.296425959524793 33.0 33.0 33.0 27.0 33.0 3 31.301319441655806 33.0 33.0 33.0 27.0 33.0 4 34.956091055224775 37.0 37.0 37.0 33.0 37.0 5 34.958834588524766 37.0 37.0 37.0 33.0 37.0 6 35.04661962284631 37.0 37.0 37.0 33.0 37.0 7 35.013338006077 37.0 37.0 37.0 33.0 37.0 8 35.020847981289556 37.0 37.0 37.0 33.0 37.0 9 35.02241432390632 37.0 37.0 37.0 33.0 37.0 10-11 34.93482739444649 37.0 37.0 37.0 33.0 37.0 12-13 34.85633272368631 37.0 37.0 37.0 33.0 37.0 14-15 36.55687495664935 40.0 37.0 40.0 33.0 40.0 16-17 36.53356952592338 40.0 37.0 40.0 33.0 40.0 18-19 36.47801948680132 40.0 37.0 40.0 33.0 40.0 20-21 36.40067316713455 40.0 37.0 40.0 33.0 40.0 22-23 36.33080688791621 40.0 37.0 40.0 33.0 40.0 24-25 36.21336526374839 40.0 37.0 40.0 33.0 40.0 26-27 36.029630062291076 40.0 37.0 40.0 33.0 40.0 28-29 35.94549475715726 40.0 37.0 40.0 33.0 40.0 30-31 35.83052907870288 40.0 37.0 40.0 30.0 40.0 32-33 35.686249599957165 40.0 37.0 40.0 27.0 40.0 34-35 35.57049357071845 38.5 37.0 40.0 27.0 40.0 36-37 35.37994821042436 37.0 37.0 40.0 27.0 40.0 38-39 35.20327116393908 37.0 37.0 40.0 27.0 40.0 40-41 34.996482786902206 37.0 37.0 40.0 27.0 40.0 42-43 34.83087770918742 37.0 37.0 40.0 27.0 40.0 44-45 34.55999510822147 37.0 35.0 40.0 24.5 40.0 46-47 34.39054706389855 37.0 37.0 40.0 22.0 40.0 48-49 34.382793838306036 37.0 37.0 40.0 22.0 40.0 50-51 33.5610409996751 37.0 33.0 38.5 22.0 40.0 52-53 33.68041414624471 37.0 33.0 38.5 22.0 40.0 54-55 34.210061001208345 37.0 33.0 40.0 22.0 40.0 56-57 34.15636314519456 37.0 33.0 40.0 22.0 40.0 58-59 34.0118972434463 37.0 33.0 40.0 22.0 40.0 60-61 34.021078698008125 37.0 33.0 40.0 22.0 40.0 62-63 33.88602971329554 37.0 33.0 40.0 22.0 40.0 64-65 33.68521709396717 37.0 33.0 40.0 22.0 40.0 66-67 33.471158219830684 37.0 33.0 40.0 18.5 40.0 68-69 33.27338775132729 37.0 33.0 40.0 15.0 40.0 70-71 33.068644673133065 37.0 33.0 40.0 15.0 40.0 72-73 32.476040141788566 37.0 33.0 38.5 10.5 40.0 74-75 32.285432721617646 37.0 33.0 37.0 10.5 40.0 76-77 31.932102965853684 37.0 33.0 37.0 6.0 40.0 78-79 31.66280751581614 37.0 33.0 37.0 6.0 38.5 80-81 31.378710176724624 37.0 33.0 37.0 4.0 37.0 82-83 31.116695202041406 37.0 33.0 37.0 2.0 37.0 84-85 30.7473873400285 37.0 33.0 37.0 2.0 37.0 86-87 30.602832972534245 37.0 33.0 37.0 2.0 37.0 88-89 30.401505742972326 37.0 33.0 37.0 2.0 37.0 90-91 30.123810671134976 35.0 33.0 37.0 2.0 37.0 92-93 29.842011737834742 33.0 33.0 37.0 2.0 37.0 94-95 29.615193718080235 33.0 33.0 37.0 2.0 37.0 96-97 29.332668562535062 33.0 30.0 37.0 2.0 37.0 98-99 29.040593243748077 33.0 27.0 37.0 2.0 37.0 100-101 28.358194641677223 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04832287361626442 1101 2 0.04336189784415723 1101 3 0.0762437190658325 1101 4 0.17268381793898868 1101 5 -0.0366554811115023 1101 6 0.03398832783036454 1101 7 0.012573635045562526 1101 8 0.09953462698874915 1101 9 0.11310069332144934 1101 10-11 0.09133742924647947 1101 12-13 0.12603931310331262 1101 14-15 0.18933320647280283 1101 16-17 0.1442312191959516 1101 18-19 0.1840313821572721 1101 20-21 0.21965746828468724 1101 22-23 0.23156803320696184 1101 24-25 0.26842230336460915 1101 26-27 0.2734378482535931 1101 28-29 0.13476731857598168 1101 30-31 0.29777426600141865 1101 32-33 0.2741563852365374 1101 34-35 0.2703489715113747 1101 36-37 0.2475157460592996 1101 38-39 0.20368043275352932 1101 40-41 0.1813097637381489 1101 42-43 0.10535018631860282 1101 44-45 0.12911960214129437 1101 46-47 0.07868330543789881 1101 48-49 0.015136247741303066 1101 50-51 0.019252686013572884 1101 52-53 0.18384980811274687 1101 54-55 0.1622726534582526 1101 56-57 0.21830241909800208 1101 58-59 0.20701138818547093 1101 60-61 0.13965771591226428 1101 62-63 0.19203174445741666 1101 64-65 0.1869849612848995 1101 66-67 0.10882737128525122 1101 68-69 0.13587256609197595 1101 70-71 0.20913122805605866 1101 72-73 0.15465035729832266 1101 74-75 0.10061844794864783 1101 76-77 0.04888098263517193 1101 78-79 0.10091705566520659 1101 80-81 0.2181451662058116 1101 82-83 0.21274984338907288 1101 84-85 0.23017368831012774 1101 86-87 0.17025492771690054 1101 88-89 0.21112323778027076 1101 90-91 0.09000774992329497 1101 92-93 0.08197161748566728 1101 94-95 -0.047219628515552614 1101 96-97 0.02636200030804403 1101 98-99 0.08953957531405976 1101 100-101 0.1474496374872949 1102 1 0.1110702730455202 1102 2 0.11731861577871072 1102 3 0.099038102257893 1102 4 0.19348506458427295 1102 5 0.12149582112120072 1102 6 0.21968063021177642 1102 7 0.17901126914578924 1102 8 0.1348224600706942 1102 9 0.20293810896664866 1102 10-11 0.21874758209418133 1102 12-13 0.09702841071057122 1102 14-15 0.29957233417515994 1102 16-17 0.349151082139322 1102 18-19 0.26234071464794084 1102 20-21 0.23787794941683416 1102 22-23 0.19206633283378238 1102 24-25 0.2880823703527611 1102 26-27 0.2312979582600363 1102 28-29 0.2786376104630506 1102 30-31 0.24375577344729749 1102 32-33 0.2695009387314542 1102 34-35 0.2046838963444344 1102 36-37 0.26369164130002076 1102 38-39 0.2378527555287988 1102 40-41 0.20598852066886764 1102 42-43 0.21235586101535375 1102 44-45 0.2981510577173907 1102 46-47 0.3276421477324831 1102 48-49 0.371673456792486 1102 50-51 0.17828327414264322 1102 52-53 0.31328164359522503 1102 54-55 0.390053507132329 1102 56-57 0.34396063877807137 1102 58-59 0.3555821585846317 1102 60-61 0.4093912503516819 1102 62-63 0.5039575033850383 1102 64-65 0.4954599628781793 1102 66-67 0.40292643019375163 1102 68-69 0.370686932430786 1102 70-71 0.42256169775206587 1102 72-73 0.3426281170190464 1102 74-75 0.3471699034893305 1102 76-77 0.266287324912053 1102 78-79 0.23994878563780375 1102 80-81 0.3238828635057516 1102 82-83 0.21057005083282831 1102 84-85 0.32984456740571844 1102 86-87 0.22002226603539654 1102 88-89 0.3245219084619819 1102 90-91 0.39508454996436626 1102 92-93 0.35034489763918586 1102 94-95 0.3074065416527709 1102 96-97 0.36615416265872014 1102 98-99 0.31426599757310214 1102 100-101 0.31424763828746904 1103 1 0.015146077822258519 1103 2 -0.009066778422102573 1103 3 0.052488857110517984 1103 4 0.0584178667250157 1103 5 0.030646412222722574 1103 6 0.1146677497900157 1103 7 0.09275987127055885 1103 8 0.11648533250459536 1103 9 0.06792333142850993 1103 10-11 0.10422790200791354 1103 12-13 0.09752390755536311 1103 14-15 0.21064375561456927 1103 16-17 0.16252350605497412 1103 18-19 0.15694466664091777 1103 20-21 0.14465839314237172 1103 22-23 0.11253347278105963 1103 24-25 0.06239541452561781 1103 26-27 0.1679055646982448 1103 28-29 0.17187239359268602 1103 30-31 0.27725984725511665 1103 32-33 0.2149688275404742 1103 34-35 0.23139579425960477 1103 36-37 0.15248317816399037 1103 38-39 0.17820092630920925 1103 40-41 0.12610619180873783 1103 42-43 0.1688582274184185 1103 44-45 0.24330317649683764 1103 46-47 0.15272052859357643 1103 48-49 0.11173730973600726 1103 50-51 0.20022367473374914 1103 52-53 0.18351513868268654 1103 54-55 0.2830523988169702 1103 56-57 0.32371016949139175 1103 58-59 0.28471362874999073 1103 60-61 0.2229146947162448 1103 62-63 0.3204189819376424 1103 64-65 0.4154291097748981 1103 66-67 0.40605167981432544 1103 68-69 0.3771071736641076 1103 70-71 0.4306344272033016 1103 72-73 0.211255303695296 1103 74-75 0.2715548383882833 1103 76-77 0.33606875713507023 1103 78-79 0.3213263417525063 1103 80-81 0.37396798412241594 1103 82-83 0.30327039630770614 1103 84-85 0.3673587957785891 1103 86-87 0.3203722340839654 1103 88-89 0.26974641720868675 1103 90-91 0.2872222506593083 1103 92-93 0.15790490642583066 1103 94-95 0.20536040178536652 1103 96-97 0.2501766984043847 1103 98-99 0.2533723059843176 1103 100-101 0.2877591507884709 1104 1 -0.031772084960771 1104 2 -0.01772467653917076 1104 3 -0.05115869547988794 1104 4 -0.08026480803854952 1104 5 -0.10378623971413958 1104 6 -0.17750091092468523 1104 7 -0.03387301187373737 1104 8 -0.07050716021794301 1104 9 -0.04745676028067436 1104 10-11 -0.08834107719734163 1104 12-13 -0.14534468181621207 1104 14-15 0.004863144230320415 1104 16-17 0.004739715955416557 1104 18-19 -0.02153385226965554 1104 20-21 0.03447311768207584 1104 22-23 0.0029974042668996503 1104 24-25 0.018401004359915873 1104 26-27 0.0643233970744106 1104 28-29 0.025736993320627732 1104 30-31 -0.15407897377269109 1104 32-33 -0.08140168778592738 1104 34-35 -0.0014135694866368453 1104 36-37 0.0033888179849057565 1104 38-39 0.006535404846694348 1104 40-41 0.08055086065129302 1104 42-43 -0.03315230226944976 1104 44-45 0.04654511015856855 1104 46-47 -0.11447726518611034 1104 48-49 -0.04599889248846978 1104 50-51 -0.06098539775922518 1104 52-53 0.04880701872100701 1104 54-55 0.052174599837435665 1104 56-57 0.14049530288446022 1104 58-59 0.1258142318392501 1104 60-61 0.18811480366539257 1104 62-63 0.12735613344350583 1104 64-65 0.22626382431727876 1104 66-67 0.26599437317972985 1104 68-69 0.2826597501413062 1104 70-71 0.26211183340875976 1104 72-73 0.1756266872102401 1104 74-75 0.19456249268520054 1104 76-77 0.12853010262614717 1104 78-79 0.20795422623545434 1104 80-81 0.3117686969346174 1104 82-83 0.27082204269342824 1104 84-85 0.3361746160001111 1104 86-87 0.2614027321084862 1104 88-89 0.19819340431585175 1104 90-91 0.41119756377205263 1104 92-93 0.42915753536575707 1104 94-95 0.4319326587506076 1104 96-97 0.18413556081429405 1104 98-99 0.33197122608775587 1104 100-101 0.4775047761860023 1105 1 0.16669722802988218 1105 2 0.10906289336295316 1105 3 0.1448404435314714 1105 4 0.18600492827151527 1105 5 0.0983814329593784 1105 6 0.1792463883010953 1105 7 0.15888349555259396 1105 8 0.10303859895697087 1105 9 0.04447165362137184 1105 10-11 0.17572599357458074 1105 12-13 0.19380716532668174 1105 14-15 0.24530075873237678 1105 16-17 0.13273081542453014 1105 18-19 0.23065391621925357 1105 20-21 0.2941783932440458 1105 22-23 0.21511484652260293 1105 24-25 0.14828673487168942 1105 26-27 0.21023300177968451 1105 28-29 0.14471601645118426 1105 30-31 0.18887040483794237 1105 32-33 0.2551934608835893 1105 34-35 0.14431496006515943 1105 36-37 0.16160660798029625 1105 38-39 0.2783054357535022 1105 40-41 0.21908296014412088 1105 42-43 0.22661073736095716 1105 44-45 0.14117670005070693 1105 46-47 0.15821437623157664 1105 48-49 0.13003637642759003 1105 50-51 0.05584100175774864 1105 52-53 0.09599627995505244 1105 54-55 0.19523987141064936 1105 56-57 0.18213295070727042 1105 58-59 0.1433908041923999 1105 60-61 0.10404440081878619 1105 62-63 0.08232524182774625 1105 64-65 0.09056380589358781 1105 66-67 0.0766585018756345 1105 68-69 0.16013498607712506 1105 70-71 0.19893594060427944 1105 72-73 0.10783416844755322 1105 74-75 0.0965934773315027 1105 76-77 0.1489919901978105 1105 78-79 0.07005786591900787 1105 80-81 0.17841368687454917 1105 82-83 0.25182842653505233 1105 84-85 0.20676133476388614 1105 86-87 0.2584779853127195 1105 88-89 0.2392361734401689 1105 90-91 0.30881798817378225 1105 92-93 0.280764258130457 1105 94-95 0.20349686716093274 1105 96-97 0.13480729612595965 1105 98-99 0.32842730928260977 1105 100-101 0.34864408426975046 1106 1 -0.037469160179774974 1106 2 0.034965559440053084 1106 3 0.025605426730976433 1106 4 -0.10661893627234775 1106 5 -0.09830058012153842 1106 6 -0.027129224418764863 1106 7 0.016462485032867846 1106 8 -0.04993667985859673 1106 9 0.05622458398087815 1106 10-11 0.025573582228425096 1106 12-13 0.005944656259671888 1106 14-15 0.06054766084093188 1106 16-17 0.04785354859375701 1106 18-19 0.005067546789042865 1106 20-21 0.03531850152034366 1106 22-23 0.008940821419663791 1106 24-25 0.023990513983413564 1106 26-27 0.12450699798412757 1106 28-29 0.12882663391777527 1106 30-31 0.09815017921605573 1106 32-33 -0.002360017357247557 1106 34-35 -0.03093837478176198 1106 36-37 -0.0020593144051233025 1106 38-39 -0.02412692055428778 1106 40-41 0.08343042322530181 1106 42-43 -0.03494812753536536 1106 44-45 -0.05246038181748247 1106 46-47 -0.20146550653878137 1106 48-49 -0.2738872888173418 1106 50-51 -0.2470797299998111 1106 52-53 -0.1163638545221275 1106 54-55 -0.043569833968106764 1106 56-57 -0.13788046405451837 1106 58-59 -0.051175759544356936 1106 60-61 -0.029494761832758343 1106 62-63 0.01089575492407846 1106 64-65 -0.03995526543626937 1106 66-67 -0.05348910017465869 1106 68-69 0.041983495088608436 1106 70-71 -0.04034199977204622 1106 72-73 -0.2299381510642462 1106 74-75 -0.15991797663519947 1106 76-77 -0.1312546510003365 1106 78-79 -0.014545765404523792 1106 80-81 0.007946699974691995 1106 82-83 0.1543619428413443 1106 84-85 0.23063771877151495 1106 86-87 0.2411986064198537 1106 88-89 0.14483845382761018 1106 90-91 0.08062489494515646 1106 92-93 0.19533148100927633 1106 94-95 0.24436965670816235 1106 96-97 0.3888568544549962 1106 98-99 0.26552113968230273 1106 100-101 0.2901299674911364 1107 1 -0.0394949465572374 1107 2 0.0055635812796275275 1107 3 -0.06219107064443108 1107 4 -0.04775854325495743 1107 5 0.08817191463553087 1107 6 -0.023343685919947177 1107 7 -0.023484938106939524 1107 8 -0.02925151781936819 1107 9 -0.04194816241309951 1107 10-11 -0.09160212460981398 1107 12-13 -0.011893648210779872 1107 14-15 0.07801864173639927 1107 16-17 0.07105776172725342 1107 18-19 0.10867921575226802 1107 20-21 0.13802761132189545 1107 22-23 0.015566369758666099 1107 24-25 0.025238626649546347 1107 26-27 0.016517290804983986 1107 28-29 -0.0051235256885107106 1107 30-31 0.06577212114172681 1107 32-33 0.05099480186585481 1107 34-35 0.030278350179052893 1107 36-37 0.08050628868945608 1107 38-39 0.1912824774624653 1107 40-41 0.1726905655245261 1107 42-43 0.1496835551130431 1107 44-45 0.05565600834075468 1107 46-47 -0.04763399410207825 1107 48-49 -0.09449660084944611 1107 50-51 -0.12808606534711942 1107 52-53 -0.006230323243791247 1107 54-55 0.08348774441599005 1107 56-57 0.03295527097695583 1107 58-59 0.07928262912005124 1107 60-61 0.03776559719707251 1107 62-63 0.11097624997376698 1107 64-65 0.06361845569762892 1107 66-67 0.07667166908547784 1107 68-69 0.16026550954655505 1107 70-71 0.12226011198756481 1107 72-73 0.12125351634568915 1107 74-75 0.23185033457625792 1107 76-77 0.16964324599944192 1107 78-79 0.09410637392004872 1107 80-81 0.09351280654851735 1107 82-83 0.17117545619461438 1107 84-85 0.1976580021430756 1107 86-87 0.29131484946605823 1107 88-89 0.2708461898207375 1107 90-91 0.3440113626579162 1107 92-93 0.2496591342088017 1107 94-95 0.29000260624342644 1107 96-97 0.23573351128433018 1107 98-99 0.20585899177467226 1107 100-101 0.2908747721051803 1108 1 -0.13726756783795935 1108 2 -0.1252141289725941 1108 3 -0.06623295753649217 1108 4 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-0.8521735564959982 2316 54-55 -0.6801025476597502 2316 56-57 -0.7134332796584744 2316 58-59 -0.8666676406300695 2316 60-61 -0.8905274439985718 2316 62-63 -0.8152453789993999 2316 64-65 -0.8140524263180922 2316 66-67 -0.8192060583540481 2316 68-69 -0.8506875296629346 2316 70-71 -0.796342609464304 2316 72-73 -0.5066526502286592 2316 74-75 -0.60204678598258 2316 76-77 -0.6040600336458617 2316 78-79 -0.5586570579578911 2316 80-81 -0.546074636940439 2316 82-83 -0.5391006984681432 2316 84-85 -0.6550146721881482 2316 86-87 -0.7090999056186753 2316 88-89 -0.6911893548493424 2316 90-91 -0.7756506836406452 2316 92-93 -0.683381206432049 2316 94-95 -0.5797685456020751 2316 96-97 -0.5256237415394303 2316 98-99 -0.5233669297277466 2316 100-101 -0.5953468621785483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 99069.0 3 15993.0 4 12124.0 5 10299.0 6 10194.0 7 10968.0 8 11056.0 9 11593.0 10 12027.0 11 12038.0 12 12617.0 13 13668.0 14 14600.0 15 16768.0 16 20329.0 17 25389.0 18 22107.0 19 16504.0 20 16617.0 21 17498.0 22 19157.0 23 21854.0 24 25937.0 25 29653.0 26 34010.0 27 40070.0 28 47743.0 29 58827.0 30 72478.0 31 91533.0 32 118411.0 33 158704.0 34 219160.0 35 321932.0 36 505343.0 37 917506.0 38 1044614.0 39 545.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.88430131204698 25.20628884990057 13.31959737381304 22.58981246423941 2 13.627380686400926 22.173611040125156 44.22392556063896 19.975082712834958 3 17.012084892132552 32.50124363216524 29.72378223830426 20.762889237397943 4 10.401662147615626 18.627121948180303 44.87598995449148 26.095225949712585 5 11.269319901500324 39.11911032927494 37.14429793669561 12.467271832529134 6 23.783097079900266 42.84538450961137 21.324795841258137 12.046722569230226 7 22.81566878035306 33.18365951274478 28.436638691047676 15.564033015854474 8 22.242965634647422 37.53004123939658 22.10027659235301 18.12671653360299 9 25.218359501914726 15.7878623049525 25.139847673423894 33.85393051970888 10-11 22.276427968039393 28.061579490344574 30.638039121105166 19.02395342051087 12-13 23.022453534131756 26.01219782279085 30.50404849924433 20.461300143833068 14-15 20.86592551659617 27.304285965141357 28.275288530492336 23.554499987770136 16-17 18.61759516816903 29.405332956252177 30.163920780830583 21.81315109474821 18-19 18.11846490611077 31.033497064090586 31.466022257712634 19.382015772086007 20-21 19.152055216254332 28.54952682386068 30.546066073082194 21.752351886802785 22-23 19.582105825475455 30.132966328257808 29.457852217180363 20.82707562908637 24-25 19.692757331614803 29.88139625978462 30.273955450007783 20.151890958592794 26-27 19.86311538147963 28.90931591762829 30.298045600624008 20.929523100268074 28-29 19.0524430296415 31.112453713675198 28.972738678027277 20.862364578656027 30-31 20.780165663365324 29.536169348018404 28.767527351978067 20.916137636638204 32-33 20.76915307737893 29.974908339995643 28.722649543008107 20.533289039617323 34-35 21.27892020681758 28.605125172337843 30.089366222634332 20.02658839821024 36-37 21.060055707865907 29.021814655135696 30.44485979943708 19.473269837561315 38-39 21.56241215789493 28.47991024438206 31.186025089226284 18.77165250849673 40-41 20.25176655064688 28.904539641811468 30.842248174127274 20.00144563341438 42-43 20.943127598757343 28.800346075077847 29.637886703002113 20.618639623162693 44-45 20.109839375853785 30.472565831951986 29.546882152902388 19.870712639291842 46-47 21.780056899572855 29.03443977658525 28.70321427533601 20.482289048505887 48-49 21.492175442554696 27.861192196756342 29.909946196761723 20.73668616392724 50-51 21.04595954695681 28.437263469745023 28.69169273361949 21.82508424967868 52-53 19.669666570885635 29.552522665972386 29.07463923055064 21.703171532591337 54-55 20.244885848150016 30.08976095087264 28.46777531799749 21.197577882979854 56-57 21.346624095799342 28.66152364302919 28.99138805366937 21.000464207502095 58-59 21.500726221243646 27.813071298825854 30.1564607324807 20.529741747449805 60-61 20.85208040475491 30.373813014292338 28.73208027295744 20.04202630799531 62-63 21.260010860479447 29.349518295026755 27.551925904684037 21.838544939809758 64-65 19.712137197177746 29.683127370150704 28.05383522994199 22.550900202729565 66-67 20.90620064771669 27.12114771571523 29.362680840430954 22.609970796137123 68-69 19.602716993799863 28.799395063409044 28.39580311212015 23.202084830670938 70-71 20.638548102167697 28.23869791819167 29.16766706682673 21.955086912813908 72-73 21.686698346269743 27.588128836930544 30.09410128146792 20.631071535331788 74-75 21.154500555462494 29.55125311579646 28.219330045791548 21.0749162829495 76-77 20.427902328133573 28.98166202869013 29.715401348279375 20.875034294896917 78-79 20.232790272461884 28.890893726907436 29.209263198416313 21.66705280221437 80-81 20.431126877131646 29.00306639876368 30.27875908506083 20.287047639043852 82-83 21.699671588225897 27.47231798373307 30.73302743078596 20.09498299725508 84-85 22.70213382875675 28.221745098402923 28.747965701756012 20.32815537108431 86-87 20.92432548958895 29.661841689660612 28.964965633217076 20.44886718753336 88-89 21.591584820976507 28.554989543125732 28.866585119883776 20.986840516013988 90-91 21.50314764846976 27.28652618062136 30.240986612153144 20.96933955875574 92-93 21.706234707524818 28.734693499220786 28.855911252846894 20.703160540407502 94-95 19.94791453936616 28.564412779713983 29.91641575950953 21.57125692141033 96-97 20.512088039315454 28.622929342477686 28.753525868556473 22.111456749650387 98-99 19.55703968989512 29.70793110492171 29.043251752530374 21.691777452652794 100-101 20.34839286029856 27.922348342891407 28.934230579620856 22.795028217189177 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 63.0 1 43.0 2 36.0 3 49.5 4 41.5 5 39.0 6 69.5 7 125.0 8 207.0 9 254.5 10 246.0 11 295.5 12 456.5 13 663.5 14 843.0 15 1112.5 16 1474.5 17 1815.5 18 2108.5 19 2401.0 20 2750.5 21 3112.5 22 3484.5 23 3935.5 24 4302.0 25 4692.5 26 5673.5 27 7710.5 28 14673.5 29 36887.0 30 68709.0 31 96469.0 32 128685.5 33 167664.5 34 222957.5 35 262504.0 36 275684.5 37 301155.5 38 284136.0 39 211439.5 40 151060.0 41 111313.5 42 89380.0 43 81994.0 44 76930.0 45 84096.0 46 95979.0 47 111874.0 48 135296.0 49 132970.0 50 110351.5 51 101540.0 52 97133.0 53 92754.5 54 88850.5 55 78247.5 56 68827.0 57 56341.5 58 43353.5 59 40351.0 60 34686.0 61 25625.5 62 19188.5 63 15951.5 64 12584.5 65 7755.0 66 5120.5 67 3799.0 68 3004.5 69 2411.5 70 1948.0 71 1571.0 72 1305.0 73 1105.0 74 906.0 75 774.0 76 671.0 77 542.5 78 445.5 79 370.5 80 301.0 81 247.0 82 206.5 83 178.5 84 146.5 85 106.5 86 81.0 87 67.0 88 53.0 89 43.5 90 42.0 91 33.5 92 21.0 93 18.5 94 15.5 95 8.5 96 4.5 97 5.5 98 5.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.920464791971642E-4 2 0.0034315461305666796 3 7.301161979929106E-5 4 8.274650243919653E-4 5 0.002166011387378968 6 0.0 7 0.0 8 0.0 9 0.0 10-11 4.867441319952737E-5 12-13 1.2168603299881844E-4 14-15 0.003419377527266798 16-17 0.004429371601156991 18-19 0.0038209414361628988 20-21 0.0 22-23 0.0 24-25 1.2168603299881843E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.3120346269775503E-4 42-43 0.0 44-45 6.327673715938558E-4 46-47 0.7556337591127629 48-49 0.5446180092895118 50-51 0.4192813953007288 52-53 0.3535344316714672 54-55 0.3448582175186514 56-57 0.30924071565989725 58-59 0.30984914582489137 60-61 0.28570663687792575 62-63 0.28012124796328003 64-65 0.27642199256011596 66-67 0.26398567998763667 68-69 0.2432017055514385 70-71 0.257365959792501 72-73 0.8369321977592733 74-75 0.5524667584179356 76-77 0.6496817301806915 78-79 0.3638290700631672 80-81 0.3145583953019456 82-83 0.2943950196340414 84-85 0.28472098001063534 86-87 0.2573294539826013 88-89 0.2597753432458776 90-91 0.2704837141497736 92-93 0.22604397489860512 94-95 0.19427175168261362 96-97 0.15143826806702954 98-99 0.1601631566330448 100-101 0.15533222112299172 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4108935.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.13050462136277 #Duplication Level Percentage of deduplicated Percentage of total 1 81.72572944968279 17.269059038207658 2 8.84948602086128 3.7398821052098907 3 3.0842608619304124 1.955159651895267 4 1.5041900151498082 1.2713717626612304 5 0.8752536358280006 0.9247275498364069 6 0.5546437610269671 0.7031941533354215 7 0.3996491846673532 0.5911352260476159 8 0.29401992563374013 0.49702315179011874 9 0.23652235175942926 0.449805298221739 >10 1.6940375807578338 7.33427038986169 >50 0.29900810736380273 4.456758744939089 >100 0.36199602924704527 16.62271469165377 >500 0.06710285247221852 9.909425442118275 >1k 0.047250624491683275 19.72060247380827 >5k 0.004875985819780584 7.221589340895908 >10k+ 0.0019736133080064267 7.333280979517623 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 34737 0.8454015456559912 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 26216 0.6380242082194048 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 21470 0.5225198256969263 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 20857 0.5076011180512712 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 19933 0.4851135391530896 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 19220 0.4677611108474581 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 18816 0.45792887938115356 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 18527 0.4508954266738218 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 16091 0.3916099913967974 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 14328 0.34870349616141405 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 14292 0.3478273567238226 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 14024 0.3413049853550859 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13632 0.33176480036797856 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 12682 0.30864445409820307 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 12666 0.30825505879260684 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 11305 0.2751321206103285 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 10138 0.24673060050840423 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 9904 0.24103569416405957 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 9837 0.23940510132187537 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 9146 0.2225880915614387 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 9075 0.22086014989285543 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 8705 0.2118553834509429 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 8631 0.21005443016256037 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 8547 0.20801010480818025 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 8337 0.20289929142222984 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7985 0.19433259469911304 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 7938 0.19318874598892416 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 7764 0.18895407204056525 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 7755 0.18873503718116738 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 7596 0.18486542133180495 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 7464 0.18165291006063614 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 7380 0.179608584706256 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 7184 0.1748384922127023 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 7106 0.17294019009792075 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 7086 0.17245344596592546 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 7063 0.1718936902141309 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 6988 0.17006839971914864 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 6944 0.16899756262875903 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 6856 0.16685588844797983 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 6790 0.16524963281239544 No Hit CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA 6789 0.16522529560579566 No Hit GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG 6774 0.1648602375067992 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 6668 0.16228049360722424 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 6623 0.1611853193102349 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 6427 0.1564152268166812 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 6403 0.15583113385828687 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 6123 0.14901671601035305 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 6059 0.14745913478796818 No Hit GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 5985 0.14565818149958565 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5870 0.14285940274061285 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 5839 0.14210494933602016 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 5782 0.14071772855983364 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 5528 0.13453607808349366 No Hit GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT 5527 0.13451174087689388 No Hit TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC 5403 0.13149392725852319 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 5165 0.12570167208777944 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5163 0.1256529976745799 No Hit GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT 5142 0.12514191633598487 No Hit GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC 5030 0.12241614919681135 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 4955 0.12059085870182906 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 4908 0.11944700999164018 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 4755 0.11572341738187633 No Hit GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA 4751 0.11562606855547727 No Hit ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA 4743 0.11543137090267916 No Hit GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT 4739 0.11533402207628012 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4664 0.11350873158129783 No Hit GATTTAACCTTTTATCCCTTATTAGAAAAAGTGGCAAAAACAGGCAAGCC 4550 0.11073429002892478 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 4514 0.10985815059133329 No Hit ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA 4443 0.10813020892275005 No Hit CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC 4406 0.10722973227855881 No Hit ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT 4405 0.10720539507195904 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 4391 0.10686467417956236 No Hit ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG 4367 0.10628058122116801 No Hit TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA 4360 0.10611022077496968 No Hit ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT 4351 0.1058911859155718 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 4336 0.10552612781657535 No Hit GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA 4304 0.10474733720538291 No Hit CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT 4268 0.10387119776779143 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 4175 0.10160783755401338 No Hit TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT 4138 0.10070736090982213 No Hit ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT 4118 0.10022061677782686 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.4337206599763685E-5 0.0 0.0 0.0 3 0.0 2.4337206599763685E-5 0.0 0.0 0.0 4 0.0 2.4337206599763685E-5 0.0 0.0 0.0 5 0.0 2.4337206599763685E-5 0.0 0.0 0.0 6 2.4337206599763685E-5 2.4337206599763685E-5 0.0 0.0 0.0 7 2.4337206599763685E-5 2.4337206599763685E-5 0.0 0.0 0.0 8 2.4337206599763685E-5 2.4337206599763685E-5 0.0 0.0 0.0 9 2.4337206599763685E-5 2.4337206599763685E-5 0.0 0.0 0.0 10-11 2.4337206599763685E-5 2.4337206599763685E-5 0.0 0.0 0.0 12-13 2.4337206599763685E-5 2.4337206599763685E-5 0.0 0.0 1.2168603299881843E-5 14-15 4.867441319952737E-5 2.4337206599763685E-5 0.0 2.4337206599763685E-5 2.4337206599763685E-5 16-17 4.867441319952737E-5 2.4337206599763685E-5 0.0 4.867441319952737E-5 4.867441319952737E-5 18-19 4.867441319952737E-5 2.4337206599763685E-5 0.0 4.867441319952737E-5 4.867441319952737E-5 20-21 4.867441319952737E-5 2.4337206599763685E-5 0.0 4.867441319952737E-5 4.867441319952737E-5 22-23 4.867441319952737E-5 2.4337206599763685E-5 0.0 1.7036044619834578E-4 4.867441319952737E-5 24-25 4.867441319952737E-5 2.4337206599763685E-5 0.0 2.555406692975187E-4 4.867441319952737E-5 26-27 4.867441319952737E-5 2.4337206599763685E-5 0.0 4.989127352951555E-4 4.867441319952737E-5 28-29 4.867441319952737E-5 2.4337206599763685E-5 0.0 0.0014480637926859393 4.867441319952737E-5 30-31 4.867441319952737E-5 4.867441319952737E-5 0.0 0.005999121426841748 4.867441319952737E-5 32-33 4.867441319952737E-5 4.867441319952737E-5 0.0 0.022803962583978574 4.867441319952737E-5 34-35 4.867441319952737E-5 4.867441319952737E-5 0.0 0.049465372414019694 4.867441319952737E-5 36-37 4.867441319952737E-5 6.084301649940921E-5 0.0 0.0936860768057903 4.867441319952737E-5 38-39 4.867441319952737E-5 7.301161979929106E-5 0.0 0.15524704089989255 4.867441319952737E-5 40-41 4.867441319952737E-5 7.301161979929106E-5 0.0 0.22023955112456148 4.867441319952737E-5 42-43 4.867441319952737E-5 7.301161979929106E-5 0.0 0.2965853682280202 7.301161979929106E-5 44-45 4.867441319952737E-5 7.301161979929106E-5 0.0 0.38518496885445985 7.301161979929106E-5 46-47 4.867441319952737E-5 7.301161979929106E-5 0.0 0.4961261251394826 7.301161979929106E-5 48-49 4.867441319952737E-5 7.301161979929106E-5 0.0 0.6152689200486257 9.734882639905474E-5 50-51 4.867441319952737E-5 7.301161979929106E-5 0.0 0.7331705174211809 9.734882639905474E-5 52-53 4.867441319952737E-5 7.301161979929106E-5 0.0 0.8516805449587301 9.734882639905474E-5 54-55 4.867441319952737E-5 7.301161979929106E-5 0.0 0.9902200935278849 9.734882639905474E-5 56-57 4.867441319952737E-5 7.301161979929106E-5 0.0 1.160738731569129 9.734882639905474E-5 58-59 4.867441319952737E-5 7.301161979929106E-5 0.0 1.360863581438986 9.734882639905474E-5 60-61 4.867441319952737E-5 7.301161979929106E-5 0.0 1.568386942115171 9.734882639905474E-5 62-63 4.867441319952737E-5 7.301161979929106E-5 0.0 1.770215396447011 9.734882639905474E-5 64-65 4.867441319952737E-5 7.301161979929106E-5 0.0 1.9863176224496129 9.734882639905474E-5 66-67 4.867441319952737E-5 7.301161979929106E-5 0.0 2.2393880652772555 9.734882639905474E-5 68-69 4.867441319952737E-5 7.301161979929106E-5 0.0 2.5166740286716633 9.734882639905474E-5 70-71 4.867441319952737E-5 7.301161979929106E-5 0.0 2.804011258391773 9.734882639905474E-5 72-73 4.867441319952737E-5 8.518022309917289E-5 0.0 3.137114605122739 9.734882639905474E-5 74-75 4.867441319952737E-5 9.734882639905474E-5 0.0 3.4473774834598263 9.734882639905474E-5 76-77 4.867441319952737E-5 1.0951742969893659E-4 0.0 3.8042096066255615 9.734882639905474E-5 78-79 4.867441319952737E-5 1.2168603299881843E-4 0.0 4.197072964162246 1.2168603299881843E-4 80-81 4.867441319952737E-5 1.2168603299881843E-4 0.0 4.6533955879078155 1.2168603299881843E-4 82-83 4.867441319952737E-5 1.2168603299881843E-4 0.0 5.1430845219016605 1.460232395985821E-4 84-85 4.867441319952737E-5 1.2168603299881843E-4 0.0 5.6188525737204404 1.460232395985821E-4 86-87 4.867441319952737E-5 1.2168603299881843E-4 0.0 6.138305911385798 1.460232395985821E-4 88-89 4.867441319952737E-5 1.2168603299881843E-4 0.0 6.69725853536257 1.460232395985821E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2765 0.0 58.584904 2 ACCTGGG 2860 0.0 56.801746 3 GTACCTG 3165 0.0 51.330463 1 CCTGGGG 2890 0.0 49.656765 4 CTGGGGG 2945 0.0 45.994835 5 GTACCCG 270 0.0 36.83921 1 GTACACG 295 0.0 33.717243 1 TCTCTGC 3705 0.0 32.725033 8 TTCTCTG 3945 0.0 31.213997 7 TATTCTC 3875 0.0 31.16713 5 GTACTAG 2645 0.0 29.72605 1 TGGGGGG 9770 0.0 28.358572 6 GCATCGT 2670 0.0 28.02684 8 CTGTGCG 3265 0.0 27.707645 9 TAGGCAT 3220 0.0 27.21098 5 ACTGTGC 3460 0.0 27.103004 8 TAGTACT 3470 0.0 27.025227 4 TATCACG 125 7.5626667E-6 26.524231 2 TGGGGGA 2325 0.0 26.074144 6 AGGGCTA 7485 0.0 25.81646 5 >>END_MODULE