FastQCFastQC Report
Sat 18 Jun 2016
SRR1931075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4108935
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA385200.9374691982228971No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC283490.6899354698967007No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC222530.5415758584645414No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC220050.5355402312277999No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC218030.5306241154946476No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC209620.5101565247442463No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA204370.4973794912793704No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG183910.447585566576254No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA156260.3802931903279073No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT152480.3710937262331967No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA151710.3692197613250149No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA140380.3416457062474826No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC139460.3394066832403043No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG134860.32821156820441305No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC120770.293920444105346No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT114560.2788070388068928No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT112950.2748887485443308No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC99300.2416684615356534No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA97030.23614391563750703No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA94260.22940250940937248No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC92240.22448639367622023No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC92020.2239509751310254No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA89530.2178910106876843No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA85430.20791275598178116No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA85350.20771805832898305No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT85320.20764504670918374No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA81390.1980805245154766No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG77290.18810226980957354No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG76360.18583890959579552No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT75570.18391627027441418No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG75460.18364856100181678No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA75370.1834295261424189No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA74980.18248037508502812No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG74320.1808741194494437No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA74100.18033870090424892No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA73780.17955991029305648No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT73740.17946256146665743No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT73450.17875678247526428No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC70530.17165031814813325No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT70090.17057948105774368No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA69800.16987370206635052No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC69730.16970334162015216No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT68950.1678050395053706No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC67470.16420313292860558No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG67010.16308362142501645No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT65840.16023616825284412No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG64900.15794847083246633No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT64630.1572913662542727No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA63230.15388415733030578No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT62950.1532027155455124No Hit
TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC61020.148505634671758No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG60440.1470940766889717No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG56200.1367751010906719No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC55780.13575293841348185No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55450.13494981059568964No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT53700.130690799440731No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA52270.12721057889696477No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT51800.1260667301867759No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA51090.12433878851819267No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT50930.12394939321259645No Hit
ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT50910.12390071879939692No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT50620.12319493980800379No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC48390.11776774273625648No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT48330.1176217194966579No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT48210.11732967301746072No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA47700.11608847548087277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47640.1159424522412742No Hit
CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC46920.1141901733660912No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA46900.11414149895289168No Hit
GATTTAACCTTTTATCCCTTATTAGAAAAAGTGGCAAAAACAGGCAAGCC46110.11221885963151035No Hit
ATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATG46080.11214584801171107No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA45680.11117235974772051No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG45490.11070995282232501No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT45410.1105152551695269No Hit
ATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACT45080.10971212735173469No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG45070.10968779014513494No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43750.10647527887396613No Hit
CCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGG43220.10518540692417865No Hit
ATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTC42780.10411456983378904No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC42530.10350613966879495No Hit
ATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTT42410.10321409318959779No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA42410.10321409318959779No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA42270.1028733722972011No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC41930.10204590727280914No Hit
ATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGAAAAAG41840.10182687241341126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTGGG31550.058.261363
TACCTGG32750.056.271622
CTGGGGG28400.052.6818735
CCTGGGG30650.052.2237934
GTACCTG38200.048.6566241
GTACCCG2550.044.740371
GTACACG2800.035.6524851
TATTCTC41650.031.588855
TCTCTGC41600.031.170498
TGGGGGG97100.030.6702526
GTACTAG26850.030.4517781
TTCTCTG44300.029.4847857
TATTCGC7050.028.9703439
TATCACG1703.6488927E-927.9395942
GGGGGGA99500.027.2572527
TGGGGGA20600.026.746066
TCTAACG13250.026.52682
CTAACGC13400.025.8753993
CTGTGCG33900.025.7805679
TAACGCC13450.025.7792114
Produced by FastQC (version 0.11.5)