##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931074_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1777890 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.710597956004026 33.0 33.0 33.0 27.0 33.0 2 30.584048506938 33.0 33.0 33.0 27.0 33.0 3 30.631177406926188 33.0 33.0 33.0 27.0 33.0 4 34.14237438761678 37.0 37.0 37.0 27.0 37.0 5 34.11371175944519 37.0 37.0 37.0 27.0 37.0 6 34.23945350949721 37.0 37.0 37.0 27.0 37.0 7 34.20378369865402 37.0 37.0 37.0 27.0 37.0 8 34.200781825647255 37.0 37.0 37.0 27.0 37.0 9 34.205486841143156 37.0 37.0 37.0 27.0 37.0 10-11 34.09102644145588 37.0 37.0 37.0 27.0 37.0 12-13 33.98479770964458 37.0 37.0 37.0 27.0 37.0 14-15 35.405444656306074 40.0 37.0 40.0 27.0 40.0 16-17 35.392375793778015 40.0 37.0 40.0 27.0 40.0 18-19 35.34058771915023 40.0 37.0 40.0 27.0 40.0 20-21 35.23068721911929 40.0 37.0 40.0 27.0 40.0 22-23 35.09673095635838 38.5 37.0 40.0 27.0 40.0 24-25 34.92584664968024 37.0 37.0 40.0 27.0 40.0 26-27 34.700813604891195 37.0 37.0 40.0 24.5 40.0 28-29 34.57617681633847 37.0 37.0 40.0 22.0 40.0 30-31 34.439864389810396 37.0 37.0 40.0 22.0 40.0 32-33 34.219158103144736 37.0 33.0 40.0 22.0 40.0 34-35 33.971907710825754 37.0 33.0 40.0 22.0 40.0 36-37 33.70255105771449 37.0 33.0 40.0 15.0 40.0 38-39 33.475241437884236 37.0 33.0 40.0 15.0 40.0 40-41 33.28336033162907 37.0 33.0 40.0 15.0 40.0 42-43 33.031848145835795 37.0 33.0 40.0 15.0 40.0 44-45 32.6436545568061 37.0 33.0 40.0 6.0 40.0 46-47 32.408553678799024 37.0 33.0 40.0 6.0 40.0 48-49 32.280040947415195 37.0 33.0 40.0 2.0 40.0 50-51 31.373808559584674 37.0 33.0 38.5 2.0 40.0 52-53 31.539159340566627 37.0 33.0 37.0 2.0 40.0 54-55 31.969339216711944 37.0 33.0 40.0 2.0 40.0 56-57 31.76329216093234 37.0 33.0 40.0 2.0 40.0 58-59 31.549679114005926 37.0 33.0 40.0 2.0 40.0 60-61 31.46647064779036 37.0 33.0 40.0 2.0 40.0 62-63 31.317524706252918 37.0 33.0 38.5 2.0 40.0 64-65 31.062852313697697 37.0 33.0 37.0 2.0 40.0 66-67 30.682209248041218 37.0 33.0 37.0 2.0 40.0 68-69 30.33506009933123 37.0 27.0 37.0 2.0 40.0 70-71 30.022210316723758 37.0 27.0 37.0 2.0 40.0 72-73 29.421673163131576 35.0 27.0 37.0 2.0 40.0 74-75 29.271436646811672 33.0 27.0 37.0 2.0 37.0 76-77 28.96517219850497 33.0 27.0 37.0 2.0 37.0 78-79 28.651980718717134 33.0 27.0 37.0 2.0 37.0 80-81 28.291275050762422 33.0 27.0 37.0 2.0 37.0 82-83 28.019856965279068 33.0 27.0 37.0 2.0 37.0 84-85 27.605590615842374 33.0 27.0 37.0 2.0 37.0 86-87 27.538422512078924 33.0 27.0 37.0 2.0 37.0 88-89 27.304910033804116 33.0 27.0 37.0 2.0 37.0 90-91 26.9275720095169 33.0 22.0 37.0 2.0 37.0 92-93 26.56429672251939 33.0 22.0 37.0 2.0 37.0 94-95 26.26079369364809 33.0 22.0 37.0 2.0 37.0 96-97 25.89004747200333 33.0 15.0 37.0 2.0 37.0 98-99 25.570981613035677 33.0 15.0 37.0 2.0 37.0 100-101 24.82029259403 33.0 2.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.08883794940265588 1101 2 -0.03704214038847908 1101 3 -0.1459755203863864 1101 4 -0.11977800476261535 1101 5 -0.22580835762643403 1101 6 -0.20984394524476357 1101 7 -0.08847967483451669 1101 8 -0.05484110349942739 1101 9 -0.07824203533587593 1101 10-11 -0.10205096493220367 1101 12-13 -0.09139032399525604 1101 14-15 0.050330082264572695 1101 16-17 0.0601045884595095 1101 18-19 -0.014793677385448234 1101 20-21 0.06986237875998569 1101 22-23 0.01634785731678079 1101 24-25 -0.048475130914276576 1101 26-27 0.09985992204667582 1101 28-29 -0.004942388618999871 1101 30-31 -0.009370691249365848 1101 32-33 -0.10851166084628261 1101 34-35 0.033590814298264604 1101 36-37 0.26677529501932185 1101 38-39 0.09847793140546202 1101 40-41 0.1423771763911006 1101 42-43 0.13101417082395272 1101 44-45 8.047700708999628E-4 1101 46-47 0.02485271663051236 1101 48-49 0.13835521310930687 1101 50-51 -0.01003562523242607 1101 52-53 0.1435611428234047 1101 54-55 0.16184652333010874 1101 56-57 0.2541260357750019 1101 58-59 0.15573956241840392 1101 60-61 0.08022530723615162 1101 62-63 -0.04211713943844586 1101 64-65 0.2502726547499954 1101 66-67 0.34932833067408353 1101 68-69 0.1678806244386486 1101 70-71 0.15215212712757165 1101 72-73 0.042955893723529925 1101 74-75 0.126000642479422 1101 76-77 0.17362872991175848 1101 78-79 0.21475352248438995 1101 80-81 0.31906714030732886 1101 82-83 0.3248704961579385 1101 84-85 0.08892750447876452 1101 86-87 0.09063450451150246 1101 88-89 0.019232100278546937 1101 90-91 0.20464831680027018 1101 92-93 0.4492047999635531 1101 94-95 0.34655623602041885 1101 96-97 0.23898009536388898 1101 98-99 0.3042380437880752 1101 100-101 0.3751493858473829 1102 1 0.13378083327045687 1102 2 0.22808962593231996 1102 3 0.08860206945641025 1102 4 0.09574782324160225 1102 5 0.10550810721072423 1102 6 0.3388367574051898 1102 7 0.231519189047674 1102 8 0.18501709966022162 1102 9 0.16996274289548552 1102 10-11 0.13826810519427823 1102 12-13 0.23470953142207662 1102 14-15 0.4160411519428351 1102 16-17 0.27705289821191315 1102 18-19 0.4111267725794576 1102 20-21 0.40595890364018317 1102 22-23 0.31920437653195677 1102 24-25 0.38601561595090317 1102 26-27 0.3144616816285861 1102 28-29 0.4050908934074613 1102 30-31 0.5515746365828775 1102 32-33 0.5916701569110856 1102 34-35 0.527328743046553 1102 36-37 0.7174243995872942 1102 38-39 0.4854861519181455 1102 40-41 0.42223742611913195 1102 42-43 0.5171775009875788 1102 44-45 0.5855832815698179 1102 46-47 0.4681351477358575 1102 48-49 0.3138523424664328 1102 50-51 0.14302220472031024 1102 52-53 0.349455259756386 1102 54-55 0.3012283351734375 1102 56-57 0.45559318407261173 1102 58-59 0.41430013228264784 1102 60-61 0.40761788574111435 1102 62-63 0.5524144765412835 1102 64-65 0.44425640515269293 1102 66-67 0.6091211214618824 1102 68-69 0.612326798133946 1102 70-71 0.6790098353756271 1102 72-73 0.46153768408956 1102 74-75 0.6010044943856876 1102 76-77 0.6054268386646875 1102 78-79 0.714984831401047 1102 80-81 0.7027726430083341 1102 82-83 0.6585158079600255 1102 84-85 0.7844051330740704 1102 86-87 0.7037663533175653 1102 88-89 0.6446507519090332 1102 90-91 0.7635056390795398 1102 92-93 0.6286310682523286 1102 94-95 0.6255001989549633 1102 96-97 0.9093666399938662 1102 98-99 1.0294924641423258 1102 100-101 0.9735439969137936 1103 1 0.06495384491582001 1103 2 -0.0472991475109481 1103 3 0.0276080705252717 1103 4 0.07558395518065453 1103 5 -0.15928851028921542 1103 6 -0.22885438195329044 1103 7 0.05321601638843276 1103 8 -0.003381295006526841 1103 9 -0.15974837284316123 1103 10-11 0.03797282086069487 1103 12-13 -0.046739833740268466 1103 14-15 -0.04814192507209469 1103 16-17 -0.029906288403701353 1103 18-19 0.008906624656972895 1103 20-21 0.04823982676505523 1103 22-23 0.04682032414310555 1103 24-25 0.02200914161162615 1103 26-27 -0.09166840803067089 1103 28-29 -0.13792483664491328 1103 30-31 -0.17087884093532324 1103 32-33 -0.21519856224443146 1103 34-35 -0.1291473800969527 1103 36-37 -0.14472157593952062 1103 38-39 -0.08867368387885932 1103 40-41 0.02657516790570469 1103 42-43 -0.16549541381938582 1103 44-45 -0.14875911282899068 1103 46-47 -0.30510388586051107 1103 48-49 -0.17006338045048608 1103 50-51 -0.2079924506441735 1103 52-53 0.006955285240177744 1103 54-55 0.1326221092647195 1103 56-57 0.09004785277604199 1103 58-59 0.052555890527379745 1103 60-61 0.21406266382097527 1103 62-63 0.2006977427187593 1103 64-65 0.16591933905064948 1103 66-67 0.225772813367211 1103 68-69 0.25227431608545814 1103 70-71 0.3480785921015297 1103 72-73 0.0301449657553583 1103 74-75 0.1687731772814054 1103 76-77 -0.02286603126166753 1103 78-79 0.04496261681587299 1103 80-81 0.19495166737229752 1103 82-83 0.20526746185810296 1103 84-85 0.40790905248121945 1103 86-87 0.3400003367545601 1103 88-89 0.19448266810085002 1103 90-91 0.20972810384273544 1103 92-93 0.08742323118550033 1103 94-95 0.22125317946641587 1103 96-97 0.07615659967122568 1103 98-99 0.16892630166640998 1103 100-101 0.32075938300779683 1104 1 0.061441314458605234 1104 2 0.07736039818178142 1104 3 -0.0299319035577561 1104 4 0.07398400318262333 1104 5 -0.08668689560933984 1104 6 -0.05322444986175867 1104 7 0.007132080445153122 1104 8 0.04265184339835315 1104 9 -0.058321371121834886 1104 10-11 -0.10274215619688931 1104 12-13 -0.05175413034947951 1104 14-15 0.07381546431626163 1104 16-17 0.10038223820517089 1104 18-19 0.10343828669446964 1104 20-21 0.1603585170885964 1104 22-23 0.10837194006735018 1104 24-25 0.057984537875036324 1104 26-27 0.2635829835321033 1104 28-29 0.07820096124292775 1104 30-31 0.11609145409610022 1104 32-33 0.21946642798983618 1104 34-35 0.3526998297715451 1104 36-37 0.15596644092116918 1104 38-39 0.004114838468474602 1104 40-41 0.008475902697213655 1104 42-43 -0.12203215356517916 1104 44-45 0.012026517126962233 1104 46-47 -0.04879467817597316 1104 48-49 0.053633637120348965 1104 50-51 0.003929936171921611 1104 52-53 0.09512054974704753 1104 54-55 0.03276846754146234 1104 56-57 0.13850052225487275 1104 58-59 0.06675533666375344 1104 60-61 0.06026237724103467 1104 62-63 0.10297163145441957 1104 64-65 0.2449792300302711 1104 66-67 0.22708093521175243 1104 68-69 0.3242898839968227 1104 70-71 0.3704366683817035 1104 72-73 0.29417130819963333 1104 74-75 0.283516463909411 1104 76-77 0.09782159012825176 1104 78-79 0.3468005672136094 1104 80-81 0.16766145694434442 1104 82-83 0.23014103905825678 1104 84-85 0.27682805018100964 1104 86-87 0.174124725266406 1104 88-89 0.31029849872754056 1104 90-91 0.4584648986135207 1104 92-93 0.1636364144763114 1104 94-95 0.1975624348801901 1104 96-97 0.17434551401191456 1104 98-99 0.2674704578528164 1104 100-101 0.2865082052037984 1105 1 -0.023894181672677206 1105 2 0.055946815347450496 1105 3 0.14035142145837298 1105 4 0.06224781561401471 1105 5 -0.027409846322029807 1105 6 -0.1650148264282123 1105 7 -0.08340542960773689 1105 8 0.032914382436992184 1105 9 -0.030254781966974065 1105 10-11 -0.04393670379018744 1105 12-13 0.08939311145001483 1105 14-15 0.1063991561227553 1105 16-17 0.12999478849395274 1105 18-19 0.19760847261565573 1105 20-21 0.1546693216734667 1105 22-23 0.10669967863287155 1105 24-25 0.08746672937031974 1105 26-27 -0.05711474608201428 1105 28-29 0.005964494612342719 1105 30-31 -0.055614778547756316 1105 32-33 -0.12048852598523752 1105 34-35 -0.09584757066632221 1105 36-37 -0.09185909972691064 1105 38-39 -0.006339677027014545 1105 40-41 0.190533402775948 1105 42-43 0.1788219806267719 1105 44-45 -0.0884927751627771 1105 46-47 -0.07072011814096157 1105 48-49 -0.04364876353342595 1105 50-51 -1.9836285524021946E-4 1105 52-53 0.11726255089576298 1105 54-55 0.12386929641559519 1105 56-57 0.17721822030364365 1105 58-59 0.1774244327984107 1105 60-61 0.11910516778286606 1105 62-63 0.11745801618461726 1105 64-65 0.19811613663395633 1105 66-67 0.09986747374259863 1105 68-69 -0.0709703157264876 1105 70-71 0.03920503915003337 1105 72-73 -0.11937175289756752 1105 74-75 0.033011506216766406 1105 76-77 0.030808079242888198 1105 78-79 0.08642032213908024 1105 80-81 0.053942159849349025 1105 82-83 0.19217270114748075 1105 84-85 -0.0018193093147758077 1105 86-87 -0.004565362043141619 1105 88-89 0.06460496559513729 1105 90-91 -0.002229895898487655 1105 92-93 0.0028759373144247036 1105 94-95 -0.18105713205588714 1105 96-97 -0.15208881421496656 1105 98-99 -0.2904247557554065 1105 100-101 -0.29845829274860947 1106 1 -0.06306112802146657 1106 2 -0.21575602681173223 1106 3 -0.12569039281830285 1106 4 -0.15150685311477474 1106 5 -0.13086115644468777 1106 6 -0.15154755970436895 1106 7 -0.1607229803643193 1106 8 -0.10072427872631096 1106 9 -0.06809855382225294 1106 10-11 0.058364311374141664 1106 12-13 0.03637470345954341 1106 14-15 0.00809726821935186 1106 16-17 -0.05951503832556426 1106 18-19 -0.049343810015720635 1106 20-21 -0.04938446626925952 1106 22-23 -0.13655213145020895 1106 24-25 -0.09598397232105782 1106 26-27 -0.09690680651166872 1106 28-29 -0.08366871945537468 1106 30-31 0.009395777407966932 1106 32-33 0.11621626904211269 1106 34-35 0.1219024635170598 1106 36-37 0.041668342813949266 1106 38-39 0.07530525948350686 1106 40-41 0.18240266724794196 1106 42-43 0.20862399185200076 1106 44-45 0.013010573499094846 1106 46-47 0.18275121133374483 1106 48-49 0.05938678520944052 1106 50-51 0.2274599421336987 1106 52-53 0.420381055646434 1106 54-55 0.38502697759890836 1106 56-57 0.3792599325208812 1106 58-59 0.2524198041410344 1106 60-61 0.22815028394417425 1106 62-63 0.24377636575259842 1106 64-65 0.3852097388808353 1106 66-67 0.19104090217440017 1106 68-69 0.24661429711167315 1106 70-71 0.2768668050008145 1106 72-73 0.28633153806146794 1106 74-75 0.35844520977726546 1106 76-77 0.2327294136570046 1106 78-79 0.3635485068352651 1106 80-81 0.30640614300692093 1106 82-83 0.38596558982876417 1106 84-85 0.4170139942512172 1106 86-87 0.4376014449552592 1106 88-89 0.5022517169318874 1106 90-91 0.39072643247384065 1106 92-93 0.40255183316407184 1106 94-95 0.29542438177349695 1106 96-97 0.40295524471519784 1106 98-99 0.4708261528026014 1106 100-101 0.5028017161096265 1107 1 -0.0011118531403120357 1107 2 0.0663320498587936 1107 3 0.03939864543088589 1107 4 0.025605607321551815 1107 5 0.07003623499652178 1107 6 0.10355360279751125 1107 7 -0.09370328398708239 1107 8 -0.20553450834933074 1107 9 -0.11178951572062346 1107 10-11 -0.07694099599901705 1107 12-13 -0.090776859536156 1107 14-15 0.08797162358045085 1107 16-17 -0.04133572972767041 1107 18-19 -0.035842667679951035 1107 20-21 -0.021223860499297587 1107 22-23 -0.09488023881255714 1107 24-25 -0.06897953577909988 1107 26-27 -0.06163765056131609 1107 28-29 -0.02201302117957482 1107 30-31 0.05888418558949837 1107 32-33 0.20497631212870715 1107 34-35 0.07863634231878791 1107 36-37 0.09988904110203833 1107 38-39 -9.2044589960949E-4 1107 40-41 0.19980023206842645 1107 42-43 0.037527853660115795 1107 44-45 0.015281411597293015 1107 46-47 -0.0627184866420265 1107 48-49 0.0486148293682831 1107 50-51 -0.0755572261032178 1107 52-53 -0.09377241083234011 1107 54-55 -0.03820074193491152 1107 56-57 -0.0996907785800687 1107 58-59 0.08020284887780704 1107 60-61 0.03380779716895432 1107 62-63 0.04693801671676212 1107 64-65 -0.06033902496183785 1107 66-67 0.06868954723382714 1107 68-69 0.047142980929361755 1107 70-71 0.04256101069011464 1107 72-73 -0.09593256160824737 1107 74-75 -0.04467819937568507 1107 76-77 0.05238976243215987 1107 78-79 -0.10570802808774715 1107 80-81 -0.014036256955694881 1107 82-83 -0.0986177957642802 1107 84-85 -0.002187575753428206 1107 86-87 0.028620644557836528 1107 88-89 0.07369658109353239 1107 90-91 0.1093032837586918 1107 92-93 -0.11305844957940181 1107 94-95 -0.32162107556659336 1107 96-97 -0.2313374539270292 1107 98-99 -0.2543157476801561 1107 100-101 -0.146300154665024 1108 1 -0.19394114298452791 1108 2 -0.22262968247431658 1108 3 -0.32069335425725143 1108 4 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-0.9946893757983624 2316 78-79 -1.070578244024059 2316 80-81 -1.1121975464815925 2316 82-83 -1.0932152717050378 2316 84-85 -0.9232839691312726 2316 86-87 -1.1502348024212772 2316 88-89 -1.1283876612359123 2316 90-91 -1.1006058715129328 2316 92-93 -1.0832529816076217 2316 94-95 -1.0503145497291513 2316 96-97 -1.261439288932916 2316 98-99 -1.1130649553099587 2316 100-101 -1.1405992054672716 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67888.0 3 9972.0 4 7676.0 5 6556.0 6 6620.0 7 7318.0 8 7416.0 9 7553.0 10 7677.0 11 7941.0 12 8748.0 13 9533.0 14 10056.0 15 11003.0 16 13212.0 17 16718.0 18 14591.0 19 11738.0 20 11624.0 21 11814.0 22 12832.0 23 14190.0 24 16336.0 25 18747.0 26 21578.0 27 25056.0 28 29740.0 29 35741.0 30 43637.0 31 54333.0 32 69713.0 33 92290.0 34 123818.0 35 172983.0 36 246209.0 37 338080.0 38 206730.0 39 223.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.49295172570508 19.64927931100039 12.234726489159572 22.623042474134955 2 15.16054385341247 21.732930895607684 39.01988489351075 24.086640357469097 3 22.113103926177537 26.473898509860298 28.5357280862057 22.87726947775646 4 12.199057864023061 15.974801378049639 40.847416156928915 30.97872460099838 5 12.522653665253355 38.77266913330536 34.273097176489905 14.431580024951373 6 28.21878743904291 37.979233810865686 18.347648054716544 15.454330695374855 7 26.633425015045926 31.987805769760786 23.549544684991762 17.829224530201532 8 25.315514458149828 31.54553993779143 20.40936165904527 22.72958394501347 9 25.149081214248355 17.278009325661316 23.37546192396605 34.19744753612428 10-11 26.14948791811865 24.589407834401406 27.570582986175463 21.690521261304486 12-13 28.00153102964556 23.378012738144154 26.759495885146904 21.860960347063386 14-15 23.336127933240093 25.051417919619585 27.58849464812087 24.023959499019455 16-17 21.241232558983267 27.20575020945673 28.153796784645856 23.399220446914146 18-19 20.594937937066625 29.645509349165344 28.449614910291043 21.309937803476984 20-21 22.86516601139553 26.610813942369887 26.997761391312174 23.526258654922408 22-23 22.108791882512417 26.91032628565322 27.371322185287056 23.60955964654731 24-25 23.667099764327375 26.49148709987682 27.85343862668669 21.98797450910911 26-27 23.15233225902615 25.30572757594677 29.435088785020447 22.10685138000664 28-29 21.010354971342434 28.9516505520589 28.245926350899097 21.792068125699565 30-31 22.46111964182261 26.461085893952944 27.65030457452373 23.427489889700713 32-33 23.249554246888167 27.007941998661334 27.421775250437314 22.320728504013186 34-35 24.094629026542698 25.92336983727902 28.151291699711457 21.830709436466822 36-37 23.261309754821728 25.287925574698093 29.29450078463797 22.156263885842208 38-39 22.61214135857674 26.931053102272916 29.034023477267993 21.422782061882344 40-41 22.309270023705114 26.86816621870969 28.346522682972168 22.476041074613033 42-43 21.82224434582567 27.24521764563612 26.564326251905346 24.368211756632864 44-45 22.72230638932863 26.312315492875353 26.927880617263778 24.037497500532233 46-47 24.021274239231516 27.130943475005797 26.87810226897081 21.969680016791873 48-49 23.993266250259722 26.336622467599835 27.294132280584215 22.375979001556225 50-51 21.35780030996423 26.967509096622837 28.989956515220523 22.684734078192413 52-53 22.391948977383446 28.26932415574844 26.3436453247562 22.995081542111915 54-55 21.6215171545199 26.673046734372125 27.17609776851865 24.52933834258932 56-57 23.84782030501035 25.361590221347 26.89075985772464 23.89982961591801 58-59 24.819031829808548 25.88107842221986 26.622776450121606 22.67711329784999 60-61 23.018942836493576 27.089635087376962 28.244133817180213 21.647288258949253 62-63 22.33097530385195 26.772864464435564 27.53483469732105 23.36132553439144 64-65 21.2451573610931 29.05605900829522 26.677935611934743 23.020848018676936 66-67 22.749232311590713 26.391029971141904 25.86723698707532 24.99250073019206 68-69 21.535481077716913 26.944134856113305 26.633202939801937 24.88718112636785 70-71 23.404723766609326 26.386776926602945 26.330959102449388 23.87754020433834 72-73 23.47019945649678 25.11607416726813 28.630134889266746 22.783591486968337 74-75 22.20567696700687 28.490825817760314 26.71044075655198 22.59305645868084 76-77 22.78170672075629 26.187445668906317 26.34064803411993 24.69019957621747 78-79 22.720838563443113 26.585823843882352 27.144805936588494 23.54853165608604 80-81 23.38189582710436 26.13356989332235 27.327445659838773 23.15708861973452 82-83 23.3182747416966 24.92188925491134 29.287715172929392 22.472120830462668 84-85 22.81720428894669 26.699832385472806 27.730532012569963 22.752431313010543 86-87 21.995278441694055 27.736025760515393 27.296431428866235 22.972264368924318 88-89 22.163484621107703 26.84150179431452 26.234262569011825 24.760751015565948 90-91 22.813098360068718 25.36627989104155 26.79697282495454 25.023648923935195 92-93 24.021005686855972 26.55514852394832 26.983114169453543 22.440731619742166 94-95 22.430834995179286 26.250357116091045 27.7516252656183 23.56718262311137 96-97 20.76836618337162 26.658251739803024 29.007278010540062 23.56610406628529 98-99 21.84455546436375 27.88880708539665 26.449325365285137 23.81731208495446 100-101 21.467063136217597 26.04904085761794 26.999874953248288 25.484021052916177 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 90.0 1 63.5 2 68.5 3 98.5 4 76.5 5 65.0 6 103.0 7 179.5 8 285.0 9 355.5 10 343.5 11 343.5 12 426.0 13 531.0 14 656.5 15 825.0 16 989.0 17 1228.0 18 1494.0 19 1691.5 20 1944.0 21 2254.0 22 2707.5 23 3214.0 24 3631.0 25 4279.5 26 5363.5 27 6619.0 28 8316.0 29 11847.5 30 16459.0 31 20736.5 32 26480.5 33 31607.0 34 35168.0 35 39494.0 36 43312.5 37 49632.5 38 52187.0 39 49363.5 40 47126.5 41 44229.0 42 43147.5 43 44893.0 44 48246.5 45 54734.5 46 63942.5 47 86832.0 48 131881.5 49 133916.5 50 92373.0 51 71757.0 52 64933.5 53 60650.5 54 56869.0 55 50801.5 56 45468.0 57 37972.0 58 30714.0 59 28490.5 60 24675.0 61 19236.0 62 14939.5 63 12351.5 64 9872.0 65 6770.0 66 5044.5 67 3984.5 68 3143.5 69 2487.0 70 2015.5 71 1690.0 72 1433.5 73 1214.5 74 1037.5 75 871.5 76 708.5 77 568.5 78 456.0 79 374.0 80 303.5 81 244.0 82 192.0 83 157.5 84 136.5 85 111.5 86 95.0 87 73.5 88 49.0 89 40.5 90 36.5 91 26.5 92 23.0 93 20.0 94 14.5 95 11.0 96 5.5 97 4.0 98 3.5 99 3.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.4997159554303136E-4 2 0.0032622940676869773 3 1.6873934832863677E-4 4 8.436967416431838E-4 5 0.0017998863821721254 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.812322472143946E-5 12-13 8.436967416431838E-5 14-15 0.003177924393522659 16-17 0.004302853382380237 18-19 0.003234170842965538 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.968625730500762E-4 42-43 0.0 44-45 6.468341685931076E-4 46-47 0.7171703536214277 48-49 0.51676425425645 50-51 0.396003127302589 52-53 0.33137595689272115 54-55 0.32082974762218136 56-57 0.2885724088666903 58-59 0.28874114821501895 60-61 0.26658004713452466 62-63 0.26199596150493 64-65 0.25760873844838544 66-67 0.2465844343575812 68-69 0.22661694480535918 70-71 0.23977861397499284 72-73 0.795718520268408 74-75 0.5253418377964891 76-77 0.6156736356017527 78-79 0.34299084870267565 80-81 0.2958563240695431 82-83 0.277098133180343 84-85 0.268520549640304 86-87 0.24315340094156557 88-89 0.24588135373954517 90-91 0.2561182075381491 92-93 0.21368026143349703 94-95 0.18319468583545664 96-97 0.139125592696961 98-99 0.1480969013831002 100-101 0.14365343187711274 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1777890.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.780568189099434 #Duplication Level Percentage of deduplicated Percentage of total 1 88.0610323523312 38.55362031703727 2 6.723874469788921 5.887500894390774 3 1.7758899394860268 2.33248411776011 4 0.7806144589982045 1.3670297820627142 5 0.4506739959460916 0.9865381805285893 6 0.29717110165017757 0.780619180777524 7 0.2261860285178673 0.6931786991463654 8 0.1613333114829535 0.5650611235642131 9 0.12828514768373445 0.5054756990234784 >10 1.011753287843789 9.11792818299243 >50 0.17589035841352438 5.447985543003601 >100 0.18368943463401 16.617164370240232 >500 0.014576452427651548 4.435305792585996 >1k 0.007868704407847295 6.716775225825391 >5k 6.449757711350242E-4 1.759529225753913 >10k+ 5.159806169080193E-4 4.233803665307464 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 31299 1.7604576211126672 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18411 1.0355533806928436 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13418 0.7547148586245493 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11840 0.6659579614036863 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7536 0.4238732430015355 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7278 0.4093616590452728 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5742 0.32296711270101075 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5592 0.3145301452845789 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5008 0.28168221880993766 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4950 0.27841992474225064 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4262 0.23972236752554996 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4228 0.23780998824449207 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3697 0.20794312359032335 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 3611 0.20310592893823579 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3452 0.19416274347681803 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 3426 0.19270033579130316 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 3399 0.19118168165634544 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3071 0.17273284623908117 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 3039 0.170932959856909 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 3036 0.1707642205085804 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 2806 0.15782753713671827 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2696 0.15164042769800157 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2635 0.14820939428198596 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2629 0.14787191558532867 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2563 0.14415964992209868 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2527 0.14213477774215502 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2465 0.13864749787669656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2453 0.137972540483382 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2439 0.1371850901911817 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2398 0.13487898576402363 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 2176 0.12239227398770451 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1881 0.10579957140205525 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 1837 0.10332472762656858 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 5.624644944287892E-5 0.0 0.0 0.0 0.0 6 1.6873934832863677E-4 0.0 0.0 0.0 0.0 7 1.6873934832863677E-4 0.0 0.0 0.0 0.0 8 1.6873934832863677E-4 0.0 0.0 0.0 0.0 9 1.6873934832863677E-4 0.0 0.0 0.0 0.0 10-11 1.6873934832863677E-4 0.0 0.0 0.0 0.0 12-13 1.6873934832863677E-4 0.0 0.0 0.0 5.624644944287892E-5 14-15 1.6873934832863677E-4 0.0 0.0 2.812322472143946E-5 5.624644944287892E-5 16-17 1.6873934832863677E-4 0.0 0.0 8.436967416431838E-5 8.436967416431838E-5 18-19 1.6873934832863677E-4 0.0 0.0 1.1249289888575784E-4 1.6873934832863677E-4 20-21 1.6873934832863677E-4 0.0 0.0 2.5310902249295515E-4 1.6873934832863677E-4 22-23 1.6873934832863677E-4 0.0 0.0 4.4997159554303136E-4 2.2498579777151568E-4 24-25 1.6873934832863677E-4 0.0 0.0 8.155735169217443E-4 2.2498579777151568E-4 26-27 1.6873934832863677E-4 0.0 0.0 0.0011811754383004571 2.2498579777151568E-4 28-29 1.6873934832863677E-4 0.0 0.0 0.0024185973260437933 2.2498579777151568E-4 30-31 1.6873934832863677E-4 0.0 0.0 0.007762010023117291 2.2498579777151568E-4 32-33 1.6873934832863677E-4 0.0 0.0 0.02393286423794498 2.2498579777151568E-4 34-35 2.2498579777151568E-4 0.0 0.0 0.04865317876809026 2.812322472143946E-4 36-37 2.2498579777151568E-4 0.0 0.0 0.08557897282734028 2.812322472143946E-4 38-39 2.2498579777151568E-4 0.0 0.0 0.1318416774941082 2.812322472143946E-4 40-41 2.812322472143946E-4 0.0 0.0 0.17787939636310457 2.812322472143946E-4 42-43 2.812322472143946E-4 0.0 0.0 0.22813559894031687 2.812322472143946E-4 44-45 2.812322472143946E-4 0.0 0.0 0.29169408681077 3.0935547193583404E-4 46-47 2.812322472143946E-4 0.0 0.0 0.3716484146938225 3.3747869665727353E-4 48-49 2.812322472143946E-4 0.0 0.0 0.45191209804881066 3.3747869665727353E-4 50-51 2.812322472143946E-4 0.0 0.0 0.5354099522467644 3.3747869665727353E-4 52-53 2.812322472143946E-4 0.0 0.0 0.6189078064447182 3.937251461001524E-4 54-55 2.812322472143946E-4 0.0 0.0 0.7164672730033916 3.937251461001524E-4 56-57 2.812322472143946E-4 0.0 0.0 0.8416437462385187 3.937251461001524E-4 58-59 2.812322472143946E-4 0.0 0.0 0.9762696229800494 3.937251461001524E-4 60-61 2.812322472143946E-4 0.0 0.0 1.112695386103752 3.937251461001524E-4 62-63 2.812322472143946E-4 0.0 0.0 1.2505835569129697 3.937251461001524E-4 64-65 2.812322472143946E-4 0.0 0.0 1.3942932352395254 3.937251461001524E-4 66-67 2.812322472143946E-4 0.0 0.0 1.5644387448042343 3.937251461001524E-4 68-69 2.812322472143946E-4 0.0 0.0 1.757364066393309 3.937251461001524E-4 70-71 2.812322472143946E-4 0.0 0.0 1.9627760997587027 3.937251461001524E-4 72-73 2.812322472143946E-4 0.0 0.0 2.171309811068176 5.062180449859103E-4 74-75 2.812322472143946E-4 0.0 0.0 2.391430290962883 5.343412697073498E-4 76-77 2.812322472143946E-4 0.0 0.0 2.632643189398669 5.624644944287892E-4 78-79 2.812322472143946E-4 0.0 0.0 2.921581200186738 5.624644944287892E-4 80-81 2.812322472143946E-4 0.0 0.0 3.2351270326060666 5.624644944287892E-4 82-83 2.812322472143946E-4 0.0 0.0 3.534386266866904 5.624644944287892E-4 84-85 2.812322472143946E-4 0.0 0.0 3.8426730562633233 5.624644944287892E-4 86-87 3.0935547193583404E-4 0.0 0.0 4.163418434211341 5.624644944287892E-4 88-89 3.3747869665727353E-4 0.0 0.0 4.515268098701269 5.905877191502286E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2245 0.0 56.53813 2 GTACCTG 2370 0.0 53.15649 1 ACCTGGG 2445 0.0 52.102642 3 CCTGGGG 2330 0.0 43.495487 4 CTGGGGG 2105 0.0 39.819454 5 GTACTAG 1165 0.0 30.490065 1 TGGGGGG 4890 0.0 29.8266 6 GAGTACG 625 0.0 29.55341 1 GTACACG 290 0.0 29.396626 1 GTACCCG 185 3.0013325E-10 28.160732 1 GTACCGG 120 5.5077326E-6 27.627386 1 GTACGTG 155 4.7226422E-8 27.50007 1 TAGTACT 1640 0.0 27.143782 4 CTGTGCG 1560 0.0 26.71509 9 GTACGCA 665 0.0 25.636997 3 CTAGTAC 1855 0.0 24.763622 3 TATCACG 135 1.3740622E-5 24.557678 2 GCATCGT 1355 0.0 24.46499 8 GGGGGGA 5115 0.0 24.348488 7 TACATGG 6620 0.0 24.252956 2 >>END_MODULE