##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931074_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1777890 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.65662948776359 33.0 33.0 33.0 33.0 33.0 2 32.6000506218045 33.0 33.0 33.0 33.0 33.0 3 32.65029951234328 33.0 33.0 33.0 33.0 33.0 4 36.57339936666498 37.0 37.0 37.0 37.0 37.0 5 36.54209596769204 37.0 37.0 37.0 37.0 37.0 6 36.56450399068559 37.0 37.0 37.0 37.0 37.0 7 36.49991394293235 37.0 37.0 37.0 37.0 37.0 8 36.518069171883525 37.0 37.0 37.0 37.0 37.0 9 36.492502348289264 37.0 37.0 37.0 37.0 37.0 10-11 36.47454088835642 37.0 37.0 37.0 37.0 37.0 12-13 36.393506909876315 37.0 37.0 37.0 37.0 37.0 14-15 38.2416257473747 40.0 37.0 40.0 37.0 40.0 16-17 38.30276788777709 40.0 37.0 40.0 37.0 40.0 18-19 38.30101468594795 40.0 37.0 40.0 37.0 40.0 20-21 38.19577167316314 40.0 37.0 40.0 35.0 40.0 22-23 38.05818807687764 40.0 37.0 40.0 33.0 40.0 24-25 38.201258795538536 40.0 37.0 40.0 37.0 40.0 26-27 38.1117515706821 40.0 37.0 40.0 35.0 40.0 28-29 38.046462379562286 40.0 37.0 40.0 33.0 40.0 30-31 37.96492415166293 40.0 37.0 40.0 33.0 40.0 32-33 37.83309316099421 40.0 37.0 40.0 33.0 40.0 34-35 37.68126627631631 40.0 37.0 40.0 33.0 40.0 36-37 37.619236848173955 40.0 37.0 40.0 33.0 40.0 38-39 37.44871420616573 40.0 37.0 40.0 33.0 40.0 40-41 37.32740439509756 40.0 37.0 40.0 33.0 40.0 42-43 37.12900545028095 40.0 37.0 40.0 33.0 40.0 44-45 36.842814797315924 40.0 37.0 40.0 33.0 40.0 46-47 36.56145740175151 38.5 37.0 40.0 33.0 40.0 48-49 36.237461260257945 37.0 37.0 40.0 33.0 40.0 50-51 35.91260145453318 37.0 37.0 40.0 33.0 40.0 52-53 35.7500911192481 37.0 37.0 40.0 33.0 40.0 54-55 35.5216762566863 37.0 37.0 40.0 27.0 40.0 56-57 35.13626630443953 37.0 35.0 40.0 27.0 40.0 58-59 34.837150779857026 37.0 33.0 40.0 27.0 40.0 60-61 34.4854411690262 37.0 33.0 40.0 27.0 40.0 62-63 34.23115350218517 37.0 33.0 38.5 27.0 40.0 64-65 33.888884857893345 37.0 33.0 37.0 27.0 40.0 66-67 33.45540697118494 37.0 33.0 37.0 27.0 40.0 68-69 33.11757560928966 37.0 33.0 37.0 24.5 40.0 70-71 32.804578179752404 37.0 33.0 37.0 22.0 40.0 72-73 32.42916940868108 37.0 33.0 37.0 22.0 38.5 74-75 32.09156387628031 37.0 33.0 37.0 22.0 37.0 76-77 30.45353733920546 33.0 30.0 37.0 22.0 37.0 78-79 31.27092564781848 33.0 33.0 37.0 22.0 37.0 80-81 31.274160662358188 33.0 33.0 37.0 22.0 37.0 82-83 31.04590722710629 33.0 33.0 37.0 18.5 37.0 84-85 30.72961234947044 33.0 33.0 37.0 15.0 37.0 86-87 30.497341230334833 33.0 33.0 37.0 15.0 37.0 88-89 30.158563803159925 33.0 30.0 37.0 15.0 37.0 90-91 29.672020766189135 33.0 27.0 37.0 6.0 37.0 92-93 29.31016063985961 33.0 27.0 37.0 2.0 37.0 94-95 28.795775047950098 33.0 27.0 37.0 2.0 37.0 96-97 28.245349262327814 33.0 27.0 37.0 2.0 37.0 98-99 27.6486067754473 33.0 27.0 37.0 2.0 37.0 100-101 25.95569748409632 33.0 21.0 33.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05932835884709675 1101 2 0.1658193370447023 1101 3 0.10210909977335803 1101 4 0.07721989031321641 1101 5 0.1436349138785431 1101 6 0.023127875618556004 1101 7 0.03601664571640839 1101 8 0.11197770401354035 1101 9 0.0640132773608002 1101 10-11 0.08442807033981126 1101 12-13 0.11070016096596191 1101 14-15 0.1388262403173286 1101 16-17 0.20529595973935955 1101 18-19 0.13797907829454203 1101 20-21 0.06389051931284229 1101 22-23 0.12699147704457658 1101 24-25 0.1657634658565783 1101 26-27 0.15723952326106172 1101 28-29 -0.035431297505475357 1101 30-31 -0.020606847779355064 1101 32-33 0.014738465871474205 1101 34-35 0.125547490963406 1101 36-37 -0.16200891187379085 1101 38-39 -0.07142813077323495 1101 40-41 -0.04170025876307193 1101 42-43 -0.1498447520205204 1101 44-45 -0.13012637796479254 1101 46-47 -0.1478837538773874 1101 48-49 -0.2451391317217002 1101 50-51 -0.2568410272906263 1101 52-53 -0.43625094856158597 1101 54-55 -0.5156776235793501 1101 56-57 -0.6116727208923436 1101 58-59 -0.5253339595317073 1101 60-61 -0.6429512735016445 1101 62-63 -0.7896632277146551 1101 64-65 -0.6552425177508923 1101 66-67 -0.8728998025921513 1101 68-69 -0.960956856327023 1101 70-71 -0.9910962288983249 1101 72-73 -1.077691080175768 1101 74-75 -1.3903494521341955 1101 76-77 -1.247667494197426 1101 78-79 -1.3511208977361626 1101 80-81 -1.628988235269123 1101 82-83 -1.6485937941595488 1101 84-85 -1.4299025620279657 1101 86-87 -1.9802928026820084 1101 88-89 -1.824440522667512 1101 90-91 -1.8929099971227181 1101 92-93 -1.9173074389738858 1101 94-95 -1.8013925471415426 1101 96-97 -2.1874269422878747 1101 98-99 -2.3573116477102936 1101 100-101 -3.5341832400984217 1102 1 0.1169867266660205 1102 2 0.22000437484007307 1102 3 0.14116042584072375 1102 4 0.20584165490649298 1102 5 0.10641499415382327 1102 6 0.09855403652085215 1102 7 0.18755336177567017 1102 8 0.17656600137759426 1102 9 0.16202172509704837 1102 10-11 0.15411683631354833 1102 12-13 0.16165076474253226 1102 14-15 0.25005586995848716 1102 16-17 0.2561771124787313 1102 18-19 0.21493277293409108 1102 20-21 0.22044677504207044 1102 22-23 0.2477304516437826 1102 24-25 0.3526548452125411 1102 26-27 0.31395180064811257 1102 28-29 0.2722284320984869 1102 30-31 0.3489971762565389 1102 32-33 0.34625083321415673 1102 34-35 0.32666174239405876 1102 36-37 0.29267652356281104 1102 38-39 0.15316251664271618 1102 40-41 0.20778673735700437 1102 42-43 0.2672153408298712 1102 44-45 0.29701812221340873 1102 46-47 0.40438389057688084 1102 48-49 0.3985671257973067 1102 50-51 0.08184736360860967 1102 52-53 0.22234156488895707 1102 54-55 0.3175910573878795 1102 56-57 0.31457514110513074 1102 58-59 0.2816333996480509 1102 60-61 0.2728013111663685 1102 62-63 0.23564418553186073 1102 64-65 0.2235228178941071 1102 66-67 0.21069998668134104 1102 68-69 0.22640142075810132 1102 70-71 0.04248558481259579 1102 72-73 -0.14222062624222787 1102 74-75 -0.20922795600711197 1102 76-77 -0.0780752593291929 1102 78-79 0.101656482313917 1102 80-81 0.01882419323716178 1102 82-83 -0.11737662290613216 1102 84-85 -0.18064356763229839 1102 86-87 -0.3356657839329493 1102 88-89 -0.6793927123400465 1102 90-91 -0.6840672264893435 1102 92-93 -0.6051108252274844 1102 94-95 -0.642801979098202 1102 96-97 -0.8579294331872624 1102 98-99 -0.6795920851623407 1102 100-101 -1.394725362833384 1103 1 0.13285693989982406 1103 2 0.207115401167016 1103 3 0.06303731437170512 1103 4 0.12800032136151884 1103 5 0.12184251752246666 1103 6 0.11059658425926955 1103 7 0.19486320536343982 1103 8 0.05618421049322109 1103 9 0.08729560304570327 1103 10-11 0.07343406000735797 1103 12-13 0.10996126217158775 1103 14-15 0.28330730323973796 1103 16-17 0.18509244221714738 1103 18-19 0.2194634270229301 1103 20-21 0.1447730199980981 1103 22-23 0.15674020975162506 1103 24-25 0.20103138206719962 1103 26-27 0.1805059260371067 1103 28-29 0.1900397457369749 1103 30-31 0.18970236373715466 1103 32-33 0.22123736321002951 1103 34-35 0.12682549395766785 1103 36-37 0.14488127199410172 1103 38-39 0.15163021266040033 1103 40-41 0.07178174399007275 1103 42-43 -0.0494781080882305 1103 44-45 0.0851327730636271 1103 46-47 0.21691274186949272 1103 48-49 0.1450763924320242 1103 50-51 0.11820982176057981 1103 52-53 0.04520682407368071 1103 54-55 0.0887762794099487 1103 56-57 0.10963467124226867 1103 58-59 0.17801921551527045 1103 60-61 0.15190365552957275 1103 62-63 0.021025517707990105 1103 64-65 0.015695216472252582 1103 66-67 -0.01794878259714494 1103 68-69 0.07263877533666374 1103 70-71 0.07858355761337776 1103 72-73 -0.11284109103746687 1103 74-75 -0.32915922986176227 1103 76-77 -0.22792668882427947 1103 78-79 -0.13808356116870968 1103 80-81 -0.15188480953110073 1103 82-83 -0.21139033464222123 1103 84-85 -0.05980980350153331 1103 86-87 -0.0032289946576931072 1103 88-89 -0.02520831016795455 1103 90-91 -0.18514428073778078 1103 92-93 -0.03899713256783599 1103 94-95 -0.07315641495258873 1103 96-97 0.03288052908216699 1103 98-99 0.1414734722555515 1103 100-101 -0.8169977962151158 1104 1 0.11864692514808439 1104 2 0.15875512184192075 1104 3 0.12100991983881926 1104 4 0.13544574042928303 1104 5 0.11870466152632275 1104 6 0.08979959474640964 1104 7 0.1253114885259663 1104 8 0.07939698092619096 1104 9 0.10336126210285812 1104 10-11 0.12240442117786188 1104 12-13 0.1473961501255161 1104 14-15 0.14648345171582378 1104 16-17 0.09467128971305527 1104 18-19 0.028272231565928507 1104 20-21 0.11603865941808067 1104 22-23 0.12921152363166044 1104 24-25 0.13934680614311645 1104 26-27 0.18075769772909922 1104 28-29 0.10153151261513926 1104 30-31 0.13101399027092242 1104 32-33 0.33183894584313833 1104 34-35 0.13825528102052687 1104 36-37 0.06764020562763307 1104 38-39 0.08642253766373642 1104 40-41 0.008546649914059401 1104 42-43 0.04353832133575253 1104 44-45 0.07197638107981419 1104 46-47 0.1255629149049966 1104 48-49 -0.035190604884569154 1104 50-51 0.004649708669575148 1104 52-53 0.06997505604319798 1104 54-55 -0.0825830797637579 1104 56-57 0.01389290274472188 1104 58-59 0.10531157375860545 1104 60-61 -0.0641028192814943 1104 62-63 -0.08372412031693699 1104 64-65 -0.023859701632915176 1104 66-67 0.09596452680111867 1104 68-69 -0.21079444277603443 1104 70-71 -0.23288291277251005 1104 72-73 -0.17413667373125463 1104 74-75 -0.4369268894214322 1104 76-77 -0.4110402233681363 1104 78-79 -0.271622789312044 1104 80-81 -0.2622538826260339 1104 82-83 -0.06924416250947729 1104 84-85 -0.09102591361696 1104 86-87 0.13838823532185174 1104 88-89 -0.14270932356179955 1104 90-91 -0.45589839917377617 1104 92-93 -0.028014636581353614 1104 94-95 -0.2377983962884116 1104 96-97 -0.2831585720673253 1104 98-99 -0.31678186071890124 1104 100-101 -0.8380100786507896 1105 1 0.014598529730086796 1105 2 0.0899623676479564 1105 3 0.04478103252498755 1105 4 0.09380560277818972 1105 5 -0.03367843683108873 1105 6 0.0242471413224834 1105 7 0.030549574332894736 1105 8 0.02570831408917229 1105 9 -0.04221623059916624 1105 10-11 0.01309492018092584 1105 12-13 0.02509167938708856 1105 14-15 0.057767393298199465 1105 16-17 0.12577574200968655 1105 18-19 0.09603640130703894 1105 20-21 1.940107358322507E-4 1105 22-23 0.05676206729114597 1105 24-25 0.07664373944844272 1105 26-27 0.13398551457483876 1105 28-29 0.21137365022662635 1105 30-31 0.07633943432686863 1105 32-33 0.14953939724495768 1105 34-35 0.10855198282941103 1105 36-37 0.11736323783485858 1105 38-39 0.025549201929941034 1105 40-41 0.13918336901537032 1105 42-43 0.2976405648381828 1105 44-45 0.25846137228587196 1105 46-47 0.24310969552981732 1105 48-49 0.1481141512470856 1105 50-51 0.14291103730328558 1105 52-53 0.08718647527870615 1105 54-55 0.2529963317937245 1105 56-57 0.19381677191059765 1105 58-59 0.14397212822667171 1105 60-61 0.20588485264032386 1105 62-63 0.1331856067867463 1105 64-65 0.22117213480917286 1105 66-67 0.2734041112633889 1105 68-69 0.11842255055602635 1105 70-71 -0.016817676881537125 1105 72-73 0.012605138908575952 1105 74-75 0.015418124038156122 1105 76-77 0.09967712013068919 1105 78-79 0.3694018860004 1105 80-81 0.13854226381867818 1105 82-83 0.17938463506296287 1105 84-85 0.44078325539524243 1105 86-87 0.1910591944671154 1105 88-89 0.15747629567168886 1105 90-91 0.07960643351728791 1105 92-93 0.30595014414151933 1105 94-95 0.0698077714520231 1105 96-97 0.1312747600430484 1105 98-99 0.1509989001413814 1105 100-101 -0.8427185798298638 1106 1 0.06911135618128128 1106 2 0.15701280398477735 1106 3 0.11124528129879963 1106 4 0.15002007415280616 1106 5 0.09774402972318086 1106 6 0.049769198265110504 1106 7 0.07639454473238771 1106 8 0.04009084465302948 1106 9 -0.030523882244118283 1106 10-11 0.082549477887369 1106 12-13 0.12375486425663951 1106 14-15 0.15784747070635774 1106 16-17 0.05136884802524122 1106 18-19 0.07671200210022988 1106 20-21 0.17212442582336251 1106 22-23 0.19675654561365974 1106 24-25 0.1560744440315034 1106 26-27 0.18740676295807646 1106 28-29 0.25309682576689596 1106 30-31 0.20687133411758651 1106 32-33 0.256205416226706 1106 34-35 0.23477236187556372 1106 36-37 0.1159172321862556 1106 38-39 0.2797131944429694 1106 40-41 0.29148844038828514 1106 42-43 0.277362956614283 1106 44-45 0.17172589400327354 1106 46-47 0.10835001607497219 1106 48-49 0.035575625443378556 1106 50-51 0.10498450570835871 1106 52-53 0.2107994318556834 1106 54-55 0.1815949575240836 1106 56-57 0.061969057682269124 1106 58-59 0.13216201897726876 1106 60-61 0.07620328464093262 1106 62-63 0.14764648105671085 1106 64-65 0.22007107044696284 1106 66-67 0.22724560248267522 1106 68-69 0.24188634333348347 1106 70-71 0.2820933292479211 1106 72-73 0.3971783636140245 1106 74-75 0.2892735372120683 1106 76-77 0.002215587053942869 1106 78-79 0.24630380622262749 1106 80-81 0.20464537918313752 1106 82-83 0.25337249653166083 1106 84-85 0.45121621640788234 1106 86-87 0.47519382526381193 1106 88-89 0.3247087053016564 1106 90-91 0.47434831145026735 1106 92-93 0.5753360407042791 1106 94-95 0.5920377571702424 1106 96-97 0.8583892386795888 1106 98-99 0.7724782582921712 1106 100-101 0.5925664113478852 1107 1 0.03800945097670194 1107 2 0.15607889222867755 1107 3 0.03523875928701159 1107 4 0.07787339282882755 1107 5 0.031883269264476155 1107 6 0.059835648023238264 1107 7 0.0506040705093298 1107 8 0.03151410403580002 1107 9 0.00270653020180589 1107 10-11 0.06946825769077236 1107 12-13 0.0660948819142746 1107 14-15 0.10189299936558882 1107 16-17 0.1217399288544101 1107 18-19 0.056204062313248926 1107 20-21 0.15297892741329377 1107 22-23 0.08895091238582609 1107 24-25 0.09232620549113335 1107 26-27 0.14878284104515416 1107 28-29 0.10235000377208081 1107 30-31 0.006148244179236428 1107 32-33 0.033153596728055845 1107 34-35 0.004582173420892843 1107 36-37 0.047163128261978216 1107 38-39 0.0013432424235233498 1107 40-41 0.056864784077653496 1107 42-43 -0.0976293581342631 1107 44-45 0.10516608307275277 1107 46-47 0.01638153421339439 1107 48-49 -0.03779085899059709 1107 50-51 -3.5231734395324565E-4 1107 52-53 0.09285756678656298 1107 54-55 0.0016826062798145358 1107 56-57 0.028553276971386765 1107 58-59 0.09669796505016848 1107 60-61 -0.07369815969448013 1107 62-63 0.08507347116794506 1107 64-65 0.20726221556171964 1107 66-67 0.12933181010004802 1107 68-69 0.17720972641806298 1107 70-71 0.039424136900215956 1107 72-73 0.07565111355685161 1107 74-75 0.12905356687098646 1107 76-77 0.051460219059514145 1107 78-79 0.24254694790423414 1107 80-81 0.32537822016852047 1107 82-83 0.27683464697568283 1107 84-85 0.4881739758066388 1107 86-87 0.4677225312150277 1107 88-89 0.3926822950887541 1107 90-91 0.5444433380473903 1107 92-93 0.7350819847359737 1107 94-95 0.7467747617174574 1107 96-97 0.8494983249831165 1107 98-99 1.1162696817974798 1107 100-101 1.6450376193218545 1108 1 0.01169061363943058 1108 2 -0.033859729448309395 1108 3 0.034403733869012854 1108 4 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-0.08874841878814266 2316 76-77 -0.10184797795870182 2316 78-79 0.007277830172430555 2316 80-81 0.2515643057362702 2316 82-83 0.17482470221520785 2316 84-85 0.1101341556026405 2316 86-87 0.24007354503725153 2316 88-89 0.22081102540298403 2316 90-91 0.3398224820406064 2316 92-93 0.34500225377137994 2316 94-95 0.3275090522065405 2316 96-97 0.6840102661048668 2316 98-99 0.8274209030766322 2316 100-101 1.1904002984798403 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 8.0 5 30.0 6 51.0 7 137.0 8 375.0 9 716.0 10 1270.0 11 2019.0 12 3088.0 13 4755.0 14 5530.0 15 6984.0 16 9148.0 17 12201.0 18 12437.0 19 9655.0 20 8538.0 21 8376.0 22 8751.0 23 9851.0 24 11175.0 25 13492.0 26 16538.0 27 20276.0 28 25048.0 29 31555.0 30 40125.0 31 51668.0 32 67525.0 33 92502.0 34 134296.0 35 211485.0 36 341766.0 37 448805.0 38 167694.0 39 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.694274077555946 21.326731948693844 13.559090179830498 23.41990379391971 2 13.791491692943191 22.867270454911058 38.698262993240284 24.642974858905465 3 20.726366648105337 26.716501020873057 29.587376046887044 22.969756284134565 4 11.589468414806316 16.862573050076215 40.116823875492855 31.43113465962461 5 12.071331747183459 39.30743746800983 34.49381007823881 14.127420706567898 6 28.44602309479214 38.654528682876894 17.94464224445832 14.954805977872645 7 26.365635669248377 32.12409091676088 23.637570378369865 17.872703035620876 8 25.665592359482307 32.230227966859594 19.91771144446507 22.186468229193032 9 24.80541540815236 17.27137224462706 23.385530038416324 34.53768230880426 10-11 26.070313686448543 25.176304495778705 27.600245234519573 21.153136583253183 12-13 27.839995725269844 23.515599952752982 27.064975898396415 21.57942842358076 14-15 22.93217022815599 25.441332100032373 27.702840088880915 23.923657582930723 16-17 21.114467149261205 27.260601049558748 28.30743184336489 23.317499957815162 18-19 20.453796354105148 29.70107824443582 28.67950210642953 21.1656232950295 20-21 22.689170871088763 26.64115890184432 27.453863849844478 23.215806377222435 22-23 21.946661491993318 26.985640281457236 27.73602416347468 23.331674063074768 24-25 23.284455168767472 26.774997328293654 28.265528238529942 21.675019264408935 26-27 22.83687404732576 25.412961431809617 29.737132218528707 22.013032302335915 28-29 20.76782028134474 29.244835169779908 28.420740315767567 21.566604233107785 30-31 22.4027358273009 26.44300266045706 27.937977040199335 23.2162844720427 32-33 23.105225857617736 27.024197222550328 27.950576244874544 21.920000674957393 34-35 23.872005579647784 26.073463487617342 28.6464010709324 21.408129861802475 36-37 23.038967540174028 25.44752487499227 29.688619655884224 21.824887928949487 38-39 22.148414298313504 27.100160415054265 29.53346892492665 21.217956361705575 40-41 22.054106562248027 26.87601580229081 28.709416773748647 22.360460861712518 42-43 21.57691052886184 27.288725264093422 27.191075386155767 23.943288820888974 44-45 22.402932689873953 26.410435966229635 27.45017970740597 23.736451636490447 46-47 23.644038720055796 27.388674214940185 27.34111784193623 21.62616922306779 48-49 23.722065888252867 26.582452560311697 27.769984515343758 21.925497036091677 50-51 21.042162338502383 27.24428957922031 29.176664473055137 22.53688360922217 52-53 22.145352074650287 28.410925310339785 26.588090376794966 22.855632238214962 54-55 21.50504249419255 26.763101204236484 27.45678866521551 24.275067636355455 56-57 23.77169003706641 25.6713576205502 27.249970470614045 23.306981871769345 58-59 24.632429452890786 26.10600205862005 26.925119101856694 22.336449386632466 60-61 22.73540545253081 27.499282857769604 28.38997350792231 21.375338181777277 62-63 22.27452204579586 26.822722440645936 27.665603608772194 23.23715190478601 64-65 21.06767010332473 29.215868248316823 26.776572228878052 22.939889419480394 66-67 22.689760530439646 26.522319705981285 26.095707952207338 24.692211811371738 68-69 21.480444594071237 27.17523462858946 26.741007556434194 24.60331322090511 70-71 23.11182882610008 26.678551711899306 26.560657462928138 23.648961999072483 72-73 23.209928623255657 25.42024534701247 28.746154149019343 22.62367188071253 74-75 21.92794992486874 29.072323714338665 26.69481241371436 22.304913947078234 76-77 22.615784273773397 26.3817911882486 26.433173056776827 24.56925148120118 78-79 22.443618707553018 26.986465339862733 27.312344541530404 23.25757141105385 80-81 23.060671590097005 26.62792270669984 27.61331188015922 22.698093823043937 82-83 22.999940376483135 25.373884449624317 29.48681026559464 22.139364908297907 84-85 22.389264825366954 27.259792902295544 27.80277823322636 22.548164039111143 86-87 21.665005986043536 28.138960579857365 27.146208234444796 23.049825199654308 88-89 21.73075949580683 27.319659821473767 26.343221459145393 24.60635922357401 90-91 22.35738564106083 25.745476798607786 27.013413098343037 24.883724461988347 92-93 23.500653027154904 27.273473823229317 26.94884451879042 22.277028630825356 94-95 21.882665616694556 26.857114732889908 27.72010743464627 23.54011221576927 96-97 20.133679860751553 27.42419900221028 28.786253124948146 23.65586801209002 98-99 21.175494546906727 28.602866319063608 26.27946610870189 23.942173025327776 100-101 19.245649682558653 27.657261778374615 27.08036925845983 26.0167192806069 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 107.0 1 80.5 2 64.5 3 74.0 4 66.5 5 66.5 6 119.0 7 223.5 8 352.0 9 439.0 10 502.5 11 574.5 12 651.5 13 823.0 14 982.0 15 1120.0 16 1295.0 17 1458.5 18 1686.5 19 1914.0 20 2149.5 21 2436.0 22 2715.5 23 3103.5 24 3580.0 25 4204.0 26 5248.0 27 6547.0 28 8497.0 29 12186.0 30 16884.0 31 21201.5 32 26772.5 33 32060.5 34 35827.0 35 40132.0 36 43988.5 37 50465.5 38 53098.0 39 49810.5 40 46927.0 41 44196.5 42 43477.5 43 45141.5 44 48793.0 45 56394.5 46 66007.5 47 91823.5 48 142696.0 49 139870.5 50 90765.0 51 70474.5 52 64592.0 53 60630.0 54 57801.5 55 51613.5 56 44373.5 57 36392.5 58 29372.0 59 27503.5 60 23468.0 61 17135.0 62 12329.5 63 9612.5 64 7289.5 65 4326.5 66 2786.0 67 2048.5 68 1505.5 69 1155.0 70 882.5 71 651.0 72 515.0 73 415.5 74 345.0 75 264.0 76 204.0 77 156.0 78 108.5 79 80.0 80 57.0 81 52.5 82 48.5 83 31.0 84 24.5 85 29.0 86 23.0 87 14.5 88 11.5 89 7.5 90 6.0 91 4.5 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08408844191710399 2 2.2498579777151568E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.0935547193583404E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.1249289888575784E-4 40-41 0.0026154598990938698 42-43 0.006187109438716682 44-45 0.0 46-47 0.0 48-49 5.624644944287892E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0024748437754866723 68-69 0.007508901000624335 70-71 0.0014342844607934124 72-73 0.0 74-75 8.436967416431838E-5 76-77 0.0014342844607934124 78-79 0.0047247017532018295 80-81 0.0036278959890656904 82-83 0.003824758562115766 84-85 0.0018842560563364437 86-87 2.5310902249295515E-4 88-89 0.0 90-91 5.624644944287892E-5 92-93 8.999431910860627E-4 94-95 0.0036560192137871294 96-97 0.0034029101912941744 98-99 0.0 100-101 1.406161236071973E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1777890.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.18658348027347 #Duplication Level Percentage of deduplicated Percentage of total 1 84.5909361489882 31.45647908778833 2 8.851367876140296 6.58304260881404 3 2.2414081192353965 2.500509304179295 4 0.9899605362325047 1.4725300049114531 5 0.5532811668693502 1.0287318149925107 6 0.37342045405796326 0.8331738532840844 7 0.2679686794912246 0.6975387768999348 8 0.2039781080736726 0.6068199155223896 9 0.16622244439976525 0.5563120324470288 >10 1.269269064088106 9.676703793985011 >50 0.21695157636323897 5.680330548651943 >100 0.23874047902696763 18.382144916387283 >500 0.023721975571669544 5.903547120570931 >1k 0.011100668055973569 7.832887390750621 >5k 0.0012165115677779256 3.1335168595720067 >10k+ 4.5619183791672206E-4 3.6557319712432 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 34807 1.9577701657582867 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17237 0.9695200490469039 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12605 0.7089864952274888 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9926 0.5583022571700161 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8060 0.4533463825096041 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7709 0.43360387875515355 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7271 0.4089679338991726 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6709 0.3773574293122747 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5342 0.30046853292385917 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5230 0.29416893058625676 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5167 0.29062540427135536 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 4731 0.26610195231426015 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4569 0.2569900275045138 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4484 0.25220907930186903 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4354 0.24489704087429484 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 4318 0.24287216869435116 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 3947 0.22200473595104306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3613 0.20321842183712155 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3610 0.2030496824887929 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 3294 0.18527580446484315 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 3233 0.18184477104882754 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 3175 0.17858247698114058 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2989 0.1681206373847651 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2932 0.16491458976652099 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2910 0.16367716787877767 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2832 0.1592899448222331 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2609 0.14674698659647112 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2571 0.1446096215176417 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2401 0.1350477251123523 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2386 0.1342040283707091 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 2165 0.12177356304383287 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2070 0.11643015034675935 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1937 0.10894937257085646 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 1833 0.10309974182879705 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.624644944287892E-5 2 0.0 0.0 0.0 0.0 5.624644944287892E-5 3 0.0 0.0 0.0 5.624644944287892E-5 5.624644944287892E-5 4 0.0 0.0 0.0 5.624644944287892E-5 1.1249289888575784E-4 5 0.0 0.0 0.0 5.624644944287892E-5 1.1249289888575784E-4 6 0.0 0.0 0.0 5.624644944287892E-5 1.1249289888575784E-4 7 0.0 0.0 0.0 5.624644944287892E-5 1.1249289888575784E-4 8 0.0 0.0 0.0 5.624644944287892E-5 1.6873934832863677E-4 9 0.0 0.0 0.0 5.624644944287892E-5 1.6873934832863677E-4 10-11 0.0 0.0 0.0 5.624644944287892E-5 1.6873934832863677E-4 12-13 0.0 0.0 0.0 5.624644944287892E-5 2.2498579777151568E-4 14-15 0.0 0.0 0.0 8.436967416431838E-5 2.2498579777151568E-4 16-17 0.0 0.0 0.0 1.406161236071973E-4 2.2498579777151568E-4 18-19 0.0 0.0 0.0 1.6873934832863677E-4 2.812322472143946E-4 20-21 0.0 0.0 0.0 2.812322472143946E-4 2.812322472143946E-4 22-23 0.0 0.0 0.0 4.4997159554303136E-4 3.0935547193583404E-4 24-25 5.624644944287892E-5 0.0 0.0 7.593270674788653E-4 3.3747869665727353E-4 26-27 5.624644944287892E-5 0.0 0.0 0.0010686825394146996 3.3747869665727353E-4 28-29 5.624644944287892E-5 0.0 0.0 0.0022498579777151567 3.3747869665727353E-4 30-31 5.624644944287892E-5 0.0 0.0 0.007537024225345775 3.3747869665727353E-4 32-33 5.624644944287892E-5 0.0 0.0 0.023707878440173462 3.937251461001524E-4 34-35 5.624644944287892E-5 0.0 0.0 0.04834382329615443 4.4997159554303136E-4 36-37 5.624644944287892E-5 0.0 0.0 0.0850165083329115 4.780948202644708E-4 38-39 5.624644944287892E-5 0.0 0.0 0.13080111817941492 5.062180449859103E-4 40-41 5.624644944287892E-5 0.0 0.0 0.17706382284618283 5.062180449859103E-4 42-43 5.624644944287892E-5 0.0 0.0 0.2274325183222809 5.062180449859103E-4 44-45 5.624644944287892E-5 0.0 0.0 0.29082226684440543 5.343412697073498E-4 46-47 5.624644944287892E-5 0.0 0.0 0.3702984999071933 5.624644944287892E-4 48-49 5.624644944287892E-5 0.0 0.0 0.44971848652053836 5.624644944287892E-4 50-51 5.624644944287892E-5 0.0 0.0 0.5320351652801917 5.624644944287892E-4 52-53 5.624644944287892E-5 0.0 0.0 0.614351844039845 6.187109438716681E-4 54-55 5.624644944287892E-5 0.0 0.0 0.7104207796882822 6.749573933145471E-4 56-57 5.624644944287892E-5 0.0 0.0 0.8337411200917941 7.030806180359866E-4 58-59 5.624644944287892E-5 0.0 0.0 0.9663702478781027 7.874502922003048E-4 60-61 5.624644944287892E-5 0.0 0.0 1.1002930440015972 7.874502922003048E-4 62-63 5.624644944287892E-5 0.0 0.0 1.236775053574743 7.874502922003048E-4 64-65 5.624644944287892E-5 0.0 0.0 1.378712968743848 7.874502922003048E-4 66-67 5.624644944287892E-5 0.0 0.0 1.547142961600549 7.874502922003048E-4 68-69 5.624644944287892E-5 0.0 0.0 1.7383527664816159 7.874502922003048E-4 70-71 8.436967416431838E-5 0.0 0.0 1.9411774631726373 8.436967416431838E-4 72-73 1.1249289888575784E-4 0.0 0.0 2.1468144823358024 9.561896405289416E-4 74-75 1.1249289888575784E-4 0.0 0.0 2.363025833994229 9.561896405289416E-4 76-77 1.1249289888575784E-4 0.0 0.0 2.6002733577442925 9.561896405289416E-4 78-79 1.1249289888575784E-4 0.0 0.0 2.8842898042061096 9.561896405289416E-4 80-81 1.1249289888575784E-4 0.0 0.0 3.1904954749731425 9.84312865250381E-4 82-83 1.1249289888575784E-4 0.0 0.0 3.48390507849192 0.0010124360899718206 84-85 1.1249289888575784E-4 0.0 0.0 3.7860610048990657 0.0010124360899718206 86-87 1.1249289888575784E-4 0.0 0.0 4.098819387026194 0.0010124360899718206 88-89 1.6873934832863677E-4 0.0 0.0 4.442344576998577 0.0010124360899718206 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2415 0.0 59.593166 2 ACCTGGG 2410 0.0 59.125553 3 CTGGGGG 1865 0.0 55.265217 5 GTACCTG 2655 0.0 54.24435 1 CCTGGGG 2285 0.0 49.680122 4 GTACCCG 250 0.0 41.827232 1 GTACTAG 1195 0.0 34.60412 1 GTACACG 235 0.0 34.384087 1 GAGTACG 480 0.0 31.687298 1 TGGGGGG 4740 0.0 31.564812 6 CTGTGCG 1750 0.0 29.041344 9 TCTAACG 630 0.0 27.895372 2 GTACGCA 535 0.0 27.52193 3 TAGGCAT 1560 0.0 27.402418 5 CTAACGC 660 0.0 27.34706 3 CGCAGTG 540 0.0 27.2671 6 GGACCGT 105 6.9103764E-5 27.141443 6 TAACGCC 650 0.0 27.037052 4 TAGTACT 1860 0.0 26.813118 4 CTAGGCA 1755 0.0 26.522837 4 >>END_MODULE