FastQCFastQC Report
Sat 18 Jun 2016
SRR1931073_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931073_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653261
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA36050.21805389469660266No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC35840.21678367783429234No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT35020.21182378341955688No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA33970.20547269910800534No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC31700.19174225969160344No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC29820.18037079444806356No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC28050.16966468089430525No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27230.16470478647956976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21060.1273846053345479No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC20930.12659828061026057No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT20740.125449036782456No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT17380.1051255669854911No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA16810.10167783550207742No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG16680.10089151077779007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG19850.055.3726042
ACCTGGG21550.051.0044633
GTACCTG22250.049.3998341
CTGGGGG19200.040.4666255
CCTGGGG22000.038.9783444
GTATCAA51250.031.5230331
GTACTAG13750.031.0103971
TGGGGGG49250.028.6649536
GGTATCA33700.028.5386641
GTACACG2900.027.772721
TAGTACT15300.027.5591664
GTACCCG1904.0745363E-1027.4287831
CAACGCA55600.027.266515
CTGTGCG16100.027.0725679
ATCAACG57100.026.6343
TATCAAC60600.026.2684332
TAGGCAT17950.026.1291395
TATTCGC2409.094947E-1225.6625399
GCATCGT16450.025.3429978
CATGGGG43950.025.1168144
Produced by FastQC (version 0.11.5)