FastQCFastQC Report
Sat 18 Jun 2016
SRR1931073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1653261
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC42690.25821694215250945No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA40980.24787374770226844No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA40190.24309531283929156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39110.23656276897598144No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC34140.20650096990130415No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC33920.20517026652174097No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT31680.19162128665709768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30310.18333463379345427No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC27760.167910571893972No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24710.1494621841318461No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG20800.12581195588597324No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT19480.11782773560859416No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC18510.1119605434350656No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG18470.11171859736605412No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA17460.10560945912351408No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC17240.10427875574395089No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG20400.055.1807672
GTACCTG21300.053.107511
ACCTGGG21400.053.046123
CTGGGGG16200.050.136115
CCTGGGG21650.044.5356374
GTACCCG2150.035.3704151
TGGGGGG39250.031.22126
TATCACG953.493081E-529.9983392
GGTATCA35050.029.8327481
GTACTAG11900.029.1564391
GTATCAA50900.028.2932911
TAGGCAT15700.027.5303235
GCTATTG17750.026.492298
CTATTGA17750.025.9570949
GGGGGGA41350.025.8450037
TAGTACT18050.025.7880464
CTGTGCG18050.025.5256759
AGGGCTA18950.025.315225
TATTCGC1951.571243E-824.3583629
ACTGTGC22650.024.116398
Produced by FastQC (version 0.11.5)