##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931073_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1653261 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56841176317593 33.0 33.0 33.0 33.0 33.0 2 32.55187353962865 33.0 33.0 33.0 33.0 33.0 3 32.59561859863627 33.0 33.0 33.0 33.0 33.0 4 36.535687347611784 37.0 37.0 37.0 37.0 37.0 5 36.45714379036341 37.0 37.0 37.0 37.0 37.0 6 36.46595304673612 37.0 37.0 37.0 37.0 37.0 7 36.39375633974309 37.0 37.0 37.0 37.0 37.0 8 36.40505159197489 37.0 37.0 37.0 37.0 37.0 9 36.401103032128624 37.0 37.0 37.0 37.0 37.0 10-11 36.36366641443789 37.0 37.0 37.0 37.0 37.0 12-13 36.263401846411426 37.0 37.0 37.0 33.0 37.0 14-15 38.08146142684065 40.0 37.0 40.0 33.0 40.0 16-17 38.05703485414584 40.0 37.0 40.0 33.0 40.0 18-19 37.99133560883611 40.0 37.0 40.0 33.0 40.0 20-21 37.89464095505791 40.0 37.0 40.0 33.0 40.0 22-23 37.73036290095756 40.0 37.0 40.0 33.0 40.0 24-25 37.910817469232015 40.0 37.0 40.0 33.0 40.0 26-27 37.83976909876904 40.0 37.0 40.0 33.0 40.0 28-29 37.73903545780128 40.0 37.0 40.0 33.0 40.0 30-31 37.57999130203882 40.0 37.0 40.0 33.0 40.0 32-33 37.428318940566555 40.0 37.0 40.0 33.0 40.0 34-35 37.32667860670517 40.0 37.0 40.0 33.0 40.0 36-37 37.25291378675236 40.0 37.0 40.0 33.0 40.0 38-39 37.0768541688215 40.0 37.0 40.0 33.0 40.0 40-41 36.85749437021741 38.5 37.0 40.0 33.0 40.0 42-43 36.62979650521001 37.0 37.0 40.0 33.0 40.0 44-45 36.33691504245246 37.0 37.0 40.0 33.0 40.0 46-47 36.00625551561429 37.0 37.0 40.0 33.0 40.0 48-49 35.679380327728055 37.0 37.0 40.0 30.0 40.0 50-51 35.3746531854317 37.0 37.0 40.0 27.0 40.0 52-53 35.1058828581815 37.0 33.0 40.0 27.0 40.0 54-55 34.81676607625778 37.0 33.0 40.0 27.0 40.0 56-57 34.45735942479742 37.0 33.0 40.0 27.0 40.0 58-59 34.13230094945686 37.0 33.0 40.0 27.0 40.0 60-61 33.84844105074758 37.0 33.0 38.5 27.0 40.0 62-63 33.4802959121397 37.0 33.0 37.0 24.5 40.0 64-65 33.0858418604201 37.0 33.0 37.0 22.0 40.0 66-67 32.689780984369676 37.0 33.0 37.0 22.0 40.0 68-69 32.331989020487384 37.0 33.0 37.0 22.0 40.0 70-71 32.022812187549334 37.0 33.0 37.0 22.0 40.0 72-73 31.655682617566132 35.0 33.0 37.0 18.5 37.0 74-75 31.22499714201206 33.0 33.0 37.0 15.0 37.0 76-77 29.681367309819805 33.0 27.0 37.0 15.0 37.0 78-79 30.459828786864264 33.0 30.0 37.0 15.0 37.0 80-81 30.507152530665152 33.0 33.0 37.0 15.0 37.0 82-83 30.231066964018385 33.0 27.0 37.0 15.0 37.0 84-85 29.895958956268853 33.0 27.0 37.0 6.0 37.0 86-87 29.5006275476165 33.0 27.0 37.0 6.0 37.0 88-89 29.109164856607638 33.0 27.0 37.0 2.0 37.0 90-91 28.62697148242171 33.0 27.0 37.0 2.0 37.0 92-93 28.148489560934422 33.0 27.0 37.0 2.0 37.0 94-95 27.567708607412865 33.0 27.0 33.0 2.0 37.0 96-97 26.98422995522183 33.0 27.0 33.0 2.0 37.0 98-99 26.186776014192557 33.0 22.0 33.0 2.0 37.0 100-101 24.464904815392124 33.0 12.0 33.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10771689752585445 1101 2 0.2434154541989102 1101 3 0.16638681722917426 1101 4 0.23818421465629314 1101 5 0.17866294042843833 1101 6 0.13602797808910339 1101 7 0.2027696950102751 1101 8 0.2474162363752157 1101 9 0.1799094256017355 1101 10-11 0.2214057857392433 1101 12-13 0.1830126589689911 1101 14-15 0.18047563250487286 1101 16-17 0.22664570340272405 1101 18-19 0.22633329038343675 1101 20-21 0.2185554957488023 1101 22-23 0.17726004317051292 1101 24-25 0.1862240998896212 1101 26-27 0.2121133159641957 1101 28-29 0.098996000976463 1101 30-31 0.07395864691745402 1101 32-33 0.06623815983456183 1101 34-35 0.1528428254183467 1101 36-37 -0.029743783169351445 1101 38-39 0.11417973883313692 1101 40-41 0.11637710533671708 1101 42-43 0.05745774187531083 1101 44-45 0.07972014136263539 1101 46-47 0.0027259018168592775 1101 48-49 -0.06564743811925666 1101 50-51 -0.001618388253923797 1101 52-53 -0.14967965150163565 1101 54-55 -0.12299524909301596 1101 56-57 -0.11988875675167066 1101 58-59 -0.3480461379984021 1101 60-61 -0.4120951734041469 1101 62-63 -0.40898866477644447 1101 64-65 -0.4322923870802384 1101 66-67 -0.48702772735028077 1101 68-69 -0.5457276958501929 1101 70-71 -0.4773451245403031 1101 72-73 -0.7064654423158778 1101 74-75 -0.891250725845083 1101 76-77 -0.8713980298947526 1101 78-79 -0.8134442916213906 1101 80-81 -0.8008086673799788 1101 82-83 -1.086013881273967 1101 84-85 -0.8812708021864282 1101 86-87 -1.0996752533371428 1101 88-89 -1.3241990767236267 1101 90-91 -1.5335632193093893 1101 92-93 -1.717829104444629 1101 94-95 -1.813422651054882 1101 96-97 -2.0647834793080087 1101 98-99 -2.293772556728854 1101 100-101 -3.386691318491227 1102 1 0.06638017087913539 1102 2 0.2542683351568016 1102 3 0.14328707686906483 1102 4 0.19256189574288385 1102 5 0.14292700522314306 1102 6 0.1522805560370415 1102 7 0.1955282216223253 1102 8 0.14957155426615287 1102 9 0.16975311923827263 1102 10-11 0.1430270465226755 1102 12-13 0.12982918868411986 1102 14-15 0.23912203050935688 1102 16-17 0.09650131344035628 1102 18-19 0.07836405410284897 1102 20-21 -0.029800712596660617 1102 22-23 0.16227104324431707 1102 24-25 0.17998975726946043 1102 26-27 0.3114742916993549 1102 28-29 0.20383317251873478 1102 30-31 0.3495575559489552 1102 32-33 0.21202272258058485 1102 34-35 0.1559052658674389 1102 36-37 0.17514326808942116 1102 38-39 0.26038639261317087 1102 40-41 0.153228803776301 1102 42-43 0.3157799424539647 1102 44-45 0.2458606454338721 1102 46-47 0.2741389365518856 1102 48-49 0.2564787148568186 1102 50-51 0.09902109210142385 1102 52-53 0.21318533492759428 1102 54-55 0.13387492515843746 1102 56-57 0.2803223716908789 1102 58-59 0.31794219769416543 1102 60-61 0.35515603544301655 1102 62-63 -0.04717227188847062 1102 64-65 0.04828499862554736 1102 66-67 -0.014807785580600807 1102 68-69 0.019384614364327035 1102 70-71 0.017851205838393724 1102 72-73 -0.23887855085463983 1102 74-75 -0.41299152380445037 1102 76-77 -0.49690232087717945 1102 78-79 -0.36738735991638194 1102 80-81 -0.6852770026744999 1102 82-83 -0.6170740595141417 1102 84-85 -0.40195056777249505 1102 86-87 -0.6313045081432982 1102 88-89 -0.5515890117182884 1102 90-91 -0.6725435336137942 1102 92-93 -0.6711812038292386 1102 94-95 -0.6461808957200752 1102 96-97 -0.7263697123404036 1102 98-99 -0.8158050452996406 1102 100-101 -1.5423829963321118 1103 1 0.10410685017714627 1103 2 0.26713121402543294 1103 3 0.15016974484089474 1103 4 0.20184092729411418 1103 5 0.15908908681947764 1103 6 0.16185951510897212 1103 7 0.19630497658994983 1103 8 0.15508907442720243 1103 9 0.14253498328972825 1103 10-11 0.16722814085694182 1103 12-13 0.2082632919333065 1103 14-15 0.07290888481984581 1103 16-17 0.02250938512035816 1103 18-19 0.24068081653618378 1103 20-21 0.1851344170923781 1103 22-23 0.20777157642757516 1103 24-25 0.271768151713502 1103 26-27 0.2626181999803734 1103 28-29 0.23520272563437317 1103 30-31 0.16625083401921614 1103 32-33 0.03725667559856305 1103 34-35 0.1763165753892011 1103 36-37 0.20735471966602148 1103 38-39 0.035241309838909274 1103 40-41 0.031197274325521107 1103 42-43 0.049413929247222654 1103 44-45 0.11389177217942148 1103 46-47 -0.023198700454820198 1103 48-49 -0.06645286460735633 1103 50-51 -0.09729770831075513 1103 52-53 -0.15661612833662986 1103 54-55 -0.23877565851579874 1103 56-57 -0.34026606700107465 1103 58-59 -0.05128577358462394 1103 60-61 -0.4242732540649783 1103 62-63 -0.5845344532823447 1103 64-65 -0.515208702323406 1103 66-67 -0.6027562775182886 1103 68-69 -0.6098132724648053 1103 70-71 -0.6782368223158244 1103 72-73 -0.7150503600365496 1103 74-75 -0.9919246147504985 1103 76-77 -1.003082547133328 1103 78-79 -0.9817042568395067 1103 80-81 -0.9479737751869273 1103 82-83 -0.8397853355280738 1103 84-85 -0.8215785888736349 1103 86-87 -1.132208493511417 1103 88-89 -1.2448863766735307 1103 90-91 -1.022808253082225 1103 92-93 -0.8621274808881445 1103 94-95 -0.7707662029394662 1103 96-97 -1.0656385539973954 1103 98-99 -1.177938680012371 1103 100-101 -2.099328534461307 1104 1 0.14641278224797105 1104 2 0.24851081146470477 1104 3 0.1327669906690403 1104 4 0.15812680573034044 1104 5 0.1297196254410693 1104 6 0.09353292693072035 1104 7 0.0828651507408864 1104 8 0.12158686723535084 1104 9 0.06346296564816356 1104 10-11 0.10561549531529835 1104 12-13 0.12268366045069712 1104 14-15 0.13305871908538336 1104 16-17 0.17139965558347825 1104 18-19 0.1589205361177477 1104 20-21 0.12234749394649924 1104 22-23 0.13951158690188947 1104 24-25 0.16453978438944716 1104 26-27 0.11833503194598194 1104 28-29 0.09257944281802821 1104 30-31 0.08544870315268582 1104 32-33 0.21351572584733702 1104 34-35 0.185267289119885 1104 36-37 0.09201347506604662 1104 38-39 0.10848748785656426 1104 40-41 -0.01979225099484694 1104 42-43 -0.046184353487468854 1104 44-45 0.10021618347092698 1104 46-47 -0.020663233496641453 1104 48-49 0.0347084924953478 1104 50-51 -0.04462053321146442 1104 52-53 0.07443709941152576 1104 54-55 0.11465903542619316 1104 56-57 0.2396586674481469 1104 58-59 0.1817717651106392 1104 60-61 0.20888775752317912 1104 62-63 -0.054774291906731776 1104 64-65 0.2054478769157413 1104 66-67 0.34997519272228317 1104 68-69 0.09630937575893483 1104 70-71 0.06120838005879037 1104 72-73 0.1004127582829426 1104 74-75 0.10112041718963738 1104 76-77 -0.06468938570799665 1104 78-79 0.08526495280688096 1104 80-81 0.1715827830382608 1104 82-83 0.12579023833636072 1104 84-85 0.18728375007830067 1104 86-87 0.395329232719849 1104 88-89 0.22740495450468856 1104 90-91 0.2653129426738978 1104 92-93 0.4312054677336228 1104 94-95 0.2850264099452495 1104 96-97 0.3190784525401611 1104 98-99 0.37503827975732307 1104 100-101 -0.16708386226277483 1105 1 0.10185082410815482 1105 2 0.16318956565901033 1105 3 0.16717126138773608 1105 4 0.10414545683930498 1105 5 0.031043885338498 1105 6 0.11790025802898185 1105 7 0.11055357750681338 1105 8 0.08375137602196503 1105 9 0.05201600332289047 1105 10-11 -0.015727368950010145 1105 12-13 0.10475101860212277 1105 14-15 0.12796376386475572 1105 16-17 0.16265624005665558 1105 18-19 0.2143850291823668 1105 20-21 0.15106741145557123 1105 22-23 0.19615965628265286 1105 24-25 0.20310808373635325 1105 26-27 0.23632440047394 1105 28-29 0.1433896570196609 1105 30-31 0.16817705813563322 1105 32-33 0.18352158264681862 1105 34-35 0.17721472098058655 1105 36-37 0.19096311083969653 1105 38-39 0.23546814129691995 1105 40-41 0.20454352987161428 1105 42-43 0.3226245774589529 1105 44-45 0.29506418288169556 1105 46-47 0.3455719279796412 1105 48-49 0.42491138421041796 1105 50-51 0.40303281649540423 1105 52-53 0.3162954753429901 1105 54-55 0.3240755579745169 1105 56-57 0.4580238059646362 1105 58-59 0.4756692562475422 1105 60-61 0.20161314515549122 1105 62-63 0.1766385140172062 1105 64-65 0.3513237906685305 1105 66-67 0.38706937742321657 1105 68-69 0.31587792014242666 1105 70-71 0.20293779308991944 1105 72-73 0.2975124740121693 1105 74-75 0.19162815283905843 1105 76-77 -0.045364603160265915 1105 78-79 0.0190572694224862 1105 80-81 0.1596804492417654 1105 82-83 0.12699683109545745 1105 84-85 0.3692983120506028 1105 86-87 0.18409742102126003 1105 88-89 0.097205894142121 1105 90-91 0.15124947111443277 1105 92-93 0.1907611866469061 1105 94-95 0.19790315370637757 1105 96-97 0.10932978501515933 1105 98-99 0.11501815984519936 1105 100-101 -0.8622119540792639 1106 1 0.1766755113229337 1106 2 0.20543895482788344 1106 3 0.1576615702897115 1106 4 0.16510810401985054 1106 5 0.18974093010632487 1106 6 0.15950338503083117 1106 7 0.18339129200633408 1106 8 0.22989874647191044 1106 9 0.234386651682577 1106 10-11 0.20882393236122 1106 12-13 0.16796141095128547 1106 14-15 0.31427930420228734 1106 16-17 0.2691002958619606 1106 18-19 0.28789239033520175 1106 20-21 0.3168697969566239 1106 22-23 0.25429207199929493 1106 24-25 0.3122597796076434 1106 26-27 0.32804402473484373 1106 28-29 0.2514982630775222 1106 30-31 0.24813924612990235 1106 32-33 0.2520863137393974 1106 34-35 0.28193115928239365 1106 36-37 0.23481681749336047 1106 38-39 0.2819161086890247 1106 40-41 0.13164983432912436 1106 42-43 0.1519426291485857 1106 44-45 -0.0054455989197848 1106 46-47 -0.06632808279139368 1106 48-49 -0.09524391712349001 1106 50-51 -0.09485478490822885 1106 52-53 0.006521185239300564 1106 54-55 0.027192497412947603 1106 56-57 0.14310847197747734 1106 58-59 0.14823755311996933 1106 60-61 0.03199979700725919 1106 62-63 0.1286386670792936 1106 64-65 0.2995788828175776 1106 66-67 0.4000973707791857 1106 68-69 0.34594682642874375 1106 70-71 0.44011459114433293 1106 72-73 0.5680298805734232 1106 74-75 0.4992694943432632 1106 76-77 0.4368412200019982 1106 78-79 0.5172453260441223 1106 80-81 0.579189630132813 1106 82-83 0.5045379452741123 1106 84-85 0.7153256421375112 1106 86-87 0.821830628125614 1106 88-89 0.6115044445874211 1106 90-91 0.6950847536273734 1106 92-93 0.8428921511081562 1106 94-95 0.9331439997429207 1106 96-97 0.8707931436509817 1106 98-99 1.2517748874910453 1106 100-101 1.0021622770671712 1107 1 0.04702364487125266 1107 2 0.13927605783080566 1107 3 0.06169969667948294 1107 4 0.03459096358058389 1107 5 0.11170224950652852 1107 6 0.1138408255856973 1107 7 0.1781724809226759 1107 8 0.053028661180619 1107 9 0.04158031141120233 1107 10-11 -0.0049065531294800735 1107 12-13 0.009797612004554423 1107 14-15 0.050499781991099724 1107 16-17 0.007369007581736753 1107 18-19 0.06640703954606408 1107 20-21 0.0332465334474179 1107 22-23 0.0756143519215442 1107 24-25 0.056231896594859165 1107 26-27 0.13183431872424478 1107 28-29 0.16011762130774798 1107 30-31 0.23254716508483142 1107 32-33 0.16454337265002295 1107 34-35 0.12710467592159347 1107 36-37 0.2570264786781493 1107 38-39 0.2787723591050266 1107 40-41 0.17807069707656353 1107 42-43 0.14854198012518083 1107 44-45 0.20843006107236306 1107 46-47 0.36137603425559206 1107 48-49 0.2945372242503268 1107 50-51 0.16335241357366925 1107 52-53 0.1254705497315527 1107 54-55 0.39867350145677705 1107 56-57 0.488918888680935 1107 58-59 0.43718846614024187 1107 60-61 0.4667919954144466 1107 62-63 0.4748161800074726 1107 64-65 0.6208802779869842 1107 66-67 0.624658383462176 1107 68-69 0.6251145062040138 1107 70-71 0.5701754931211447 1107 72-73 0.6242580874187418 1107 74-75 0.5044623268319235 1107 76-77 0.44059028782888277 1107 78-79 0.6942982802688213 1107 80-81 0.5182384128403754 1107 82-83 0.6716112137413255 1107 84-85 0.9070578608929019 1107 86-87 0.7670952566215128 1107 88-89 0.7475759105136888 1107 90-91 0.9242176594401919 1107 92-93 0.9668056345762643 1107 94-95 0.9360756756984934 1107 96-97 0.928978404652657 1107 98-99 1.1534421699466293 1107 100-101 1.810421908164333 1108 1 -7.91895687605404E-4 1108 2 0.0333144168663182 1108 3 0.07214995912177358 1108 4 0.006223706983391253 1108 5 0.0413826209911079 1108 6 0.07466419875967034 1108 7 0.10898889779486609 1108 8 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0.26675877300356277 2316 88-89 0.07358375324861655 2316 90-91 0.25531143272229073 2316 92-93 0.46435274431837215 2316 94-95 0.596812861840494 2316 96-97 0.6273734612543755 2316 98-99 0.7670588253361075 2316 100-101 1.1180890527883705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 10.0 5 28.0 6 56.0 7 182.0 8 506.0 9 980.0 10 1665.0 11 2412.0 12 3656.0 13 5007.0 14 6309.0 15 8004.0 16 10102.0 17 13203.0 18 13423.0 19 10566.0 20 9499.0 21 9853.0 22 10635.0 23 11950.0 24 13807.0 25 16071.0 26 19376.0 27 24050.0 28 28940.0 29 36123.0 30 44886.0 31 56460.0 32 73255.0 33 98851.0 34 139360.0 35 205490.0 36 303693.0 37 369111.0 38 105734.0 39 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.23781158429883 23.422761959008064 14.748732053694704 25.5906944029984 2 13.566529160567606 21.960913588909186 39.723334502740045 24.74922274778317 3 16.00951089997284 28.108084567409502 30.654687916789907 25.22771661582775 4 11.624661804760409 18.37247718297353 37.98281094152708 32.02005007073898 5 12.513390202756854 38.09035596920268 35.26896237194248 14.127291456097979 6 29.61849338973096 38.86301074059087 18.227430514601142 13.291065355077025 7 26.256108382161074 32.012791688668635 23.864471489982524 17.866628439187764 8 26.257741518126902 35.40578287396848 19.903995799816244 18.432479808088377 9 26.32209917248396 14.727922572418993 21.730144242197692 37.219834012899355 10-11 25.4920426962228 26.43000711926308 27.8900306727129 20.187919511801223 12-13 25.340009835107708 24.18136035387032 28.402049041258458 22.076580769763517 14-15 22.57573306033694 25.3079450720774 26.107276089780328 26.009045777805333 16-17 21.729085728145765 27.39727725991238 27.35345477816267 23.52018223377918 18-19 21.465636702250883 27.633870272146986 29.39953219727557 21.500960828326562 20-21 22.46239402006096 26.98445677966153 28.62772423712892 21.92542496314859 22-23 22.787327590743388 26.654200395460848 28.35620026118078 22.202271752614983 24-25 22.199398643045473 26.80015436159203 29.145216635485866 21.855230359876632 26-27 22.24252552984677 26.90107611562845 28.373801837701368 22.482596516823417 28-29 21.709578826331715 27.260426514627756 28.801955650075822 22.228039008964707 30-31 22.553486715043782 26.99244099993891 28.506176580709276 21.94789570430803 32-33 22.837652373097775 27.25277497019527 27.880383073211068 22.02918958349589 34-35 22.53561294919556 26.963588931209287 28.743616404185424 21.757181715409725 36-37 22.527719458694058 26.652567259495026 28.484371191239617 22.3353420905713 38-39 22.481921319671834 26.68210888853202 28.920686679858786 21.91528311193736 40-41 22.145684324117028 26.104282482912804 29.272903073805136 22.477130119165032 42-43 22.424460738456904 26.386379810587396 28.529755223848095 22.6594042271076 44-45 22.19117247669908 27.26227135340397 28.355746612301385 22.190809557595564 46-47 22.450478176162143 26.77623194401852 27.97994388060929 22.793345999210047 48-49 23.131817033946618 26.510147390832945 28.033473309511674 22.32456226570876 50-51 22.577499862393175 26.86414909684557 27.521395593315273 23.036955447445987 52-53 22.069201414658664 27.275034008544324 27.43865003771334 23.217114539083667 54-55 22.36319613176625 27.20048437603016 27.319098436363042 23.11722105584055 56-57 23.170872596643846 26.836960407340406 27.102405488304633 22.889761507711125 58-59 22.320946299465117 27.13990712900141 27.86798333717423 22.671163234359245 60-61 22.693361786191048 27.708510634437033 27.015304903460496 22.582822675911427 62-63 22.950096808670864 26.96688544639957 26.743992630322737 23.33902511460683 64-65 22.024834554253683 27.247300940383884 27.270497519750357 23.457366985612072 66-67 22.603030565144568 26.725069757679393 27.115730908655394 23.556168768520642 68-69 22.25078396074389 26.78154474141394 27.279898181888573 23.6877731159536 70-71 22.242976069429684 26.574467969279738 27.375833287463834 23.806722673826744 72-73 22.763828578790644 26.719918996456094 27.489428468947132 23.02682395580613 74-75 22.764726287675952 27.257003513362545 27.113680580695288 22.86458961826622 76-77 22.48878858667841 27.066353904426858 27.00269049724599 23.442167011648742 78-79 22.185256405991126 27.18976589951844 27.2054931740604 23.419484520430032 80-81 21.900742836351892 27.43900355101242 27.63256866041754 23.02768495221815 82-83 22.66693483507643 26.531554146831276 28.31020632837121 22.491304689721083 84-85 22.818128203964978 27.083821016498057 27.01474390963118 23.08330686990579 86-87 21.865965746996913 27.42989366743757 27.481004913635605 23.223135671929914 88-89 22.418722754604385 26.589903227621047 27.336034661193846 23.655339356580722 90-91 22.178651767627734 26.54326812281908 27.722634236215576 23.555445873337604 92-93 22.557183906394744 26.74090672228269 27.29010086554255 23.411808505780012 94-95 21.256725208633068 26.87204191382599 27.877787432445473 23.99344544509547 96-97 21.636630115508755 27.09420109266348 27.230148898384233 24.039019893443538 98-99 21.310095623135126 27.474125380082153 27.028521207480242 24.187257789302475 100-101 19.923556931425946 27.92930195647197 27.043655960221543 25.103485151880545 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 110.0 1 72.0 2 54.0 3 89.5 4 75.5 5 58.5 6 109.0 7 214.0 8 337.0 9 400.5 10 402.5 11 451.0 12 554.5 13 656.0 14 779.5 15 924.0 16 1084.0 17 1256.5 18 1421.5 19 1630.0 20 1899.5 21 2131.5 22 2410.0 23 2854.0 24 3390.5 25 3999.0 26 5018.0 27 6312.0 28 8083.5 29 11668.0 30 16596.5 31 21041.0 32 27050.5 33 33283.0 34 37900.5 35 42963.5 36 46858.0 37 52771.0 38 55321.5 39 52492.5 40 49863.5 41 47081.5 42 46551.5 43 48237.0 44 52001.0 45 59282.5 46 66002.0 47 70572.5 48 73459.0 49 71009.5 50 67813.0 51 67741.5 52 66213.5 53 63232.5 54 60840.0 55 54863.5 56 47472.0 57 39740.0 58 32837.5 59 30070.0 60 25169.5 61 18354.5 62 13324.5 63 10220.5 64 7740.5 65 4919.5 66 3261.0 67 2420.0 68 1903.5 69 1505.0 70 1173.0 71 865.0 72 636.5 73 503.0 74 415.0 75 326.0 76 238.5 77 183.5 78 129.0 79 88.0 80 71.0 81 65.0 82 46.0 83 30.5 84 34.0 85 26.0 86 16.5 87 16.0 88 11.5 89 7.5 90 5.5 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07137409035838867 2 6.048651725287174E-5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.117028103850511E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.072977587930762E-5 40-41 0.0029940826040171513 42-43 0.005897435432154995 44-45 0.0 46-47 0.0 48-49 2.117028103850511E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0022984876556091265 68-69 0.007076922518585995 70-71 0.0012097303450574349 72-73 0.0 74-75 9.072977587930762E-5 76-77 0.002117028103850511 78-79 0.004959894414735483 80-81 0.0042642994663274585 82-83 0.004294542724953894 84-85 0.002177514621103383 86-87 2.7218932763792286E-4 88-89 0.0 90-91 0.0 92-93 0.0012399736036838708 94-95 0.0035989477765458688 96-97 0.0032057854144022027 98-99 0.0 100-101 2.7218932763792286E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1653261.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.20498018032149 #Duplication Level Percentage of deduplicated Percentage of total 1 84.77918580178631 36.62883042269971 2 8.991724280219334 7.7697453862758366 3 2.233745809200461 2.895268302431463 4 0.9634706595538408 1.6650692300137988 5 0.5283983484530055 1.1414720086113355 6 0.34373626608848334 0.8910671138166382 7 0.2470962975069089 0.7473053445491775 8 0.18153248982574188 0.6274486100004473 9 0.15717520929930817 0.6111676622353043 >10 1.140011535237149 10.17086471704049 >50 0.19416201068840516 5.9400338668923425 >100 0.2106182808727994 19.00273306803832 >500 0.021540486589624104 6.180591869181134 >1k 0.00760252467869086 5.728402398214069 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 4269 0.25821694215250945 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 4098 0.24787374770226844 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 4019 0.24309531283929156 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3911 0.23656276897598144 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 3414 0.20650096990130415 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 3392 0.20517026652174097 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 3168 0.19162128665709768 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3031 0.18333463379345427 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2776 0.167910571893972 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2471 0.1494621841318461 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 2080 0.12581195588597324 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 1948 0.11782773560859416 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 1851 0.1119605434350656 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 1847 0.11171859736605412 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 1746 0.10560945912351408 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 1724 0.10427875574395089 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 6.048651725287175E-5 0.0 2 0.0 0.0 0.0 6.048651725287175E-5 0.0 3 0.0 0.0 0.0 6.048651725287175E-5 0.0 4 0.0 1.209730345057435E-4 0.0 6.048651725287175E-5 0.0 5 0.0 1.209730345057435E-4 0.0 6.048651725287175E-5 0.0 6 0.0 1.209730345057435E-4 0.0 6.048651725287175E-5 6.048651725287175E-5 7 0.0 1.209730345057435E-4 0.0 6.048651725287175E-5 6.048651725287175E-5 8 0.0 1.209730345057435E-4 0.0 6.048651725287175E-5 6.048651725287175E-5 9 0.0 1.8145955175861525E-4 0.0 6.048651725287175E-5 6.048651725287175E-5 10-11 0.0 1.8145955175861525E-4 0.0 1.209730345057435E-4 6.048651725287175E-5 12-13 0.0 1.8145955175861525E-4 0.0 1.8145955175861525E-4 6.048651725287175E-5 14-15 0.0 1.8145955175861525E-4 0.0 1.8145955175861525E-4 6.048651725287175E-5 16-17 0.0 1.8145955175861525E-4 0.0 1.8145955175861525E-4 6.048651725287175E-5 18-19 0.0 1.8145955175861525E-4 0.0 1.8145955175861525E-4 6.048651725287175E-5 20-21 0.0 1.8145955175861525E-4 0.0 2.1170281038505114E-4 6.048651725287175E-5 22-23 0.0 1.8145955175861525E-4 0.0 2.7218932763792286E-4 6.048651725287175E-5 24-25 0.0 2.41946069011487E-4 0.0 3.629191035172305E-4 6.048651725287175E-5 26-27 0.0 2.41946069011487E-4 0.0 6.048651725287174E-4 6.048651725287175E-5 28-29 0.0 2.41946069011487E-4 0.0 0.001179487086430999 6.048651725287175E-5 30-31 0.0 2.41946069011487E-4 0.0 0.005322813518252714 6.048651725287175E-5 32-33 0.0 2.41946069011487E-4 0.0 0.01760157652058568 6.048651725287175E-5 34-35 0.0 2.41946069011487E-4 0.0 0.03704799181738395 6.048651725287175E-5 36-37 0.0 2.41946069011487E-4 0.0 0.0661722498746417 6.048651725287175E-5 38-39 0.0 2.41946069011487E-4 0.0 0.11047862376237025 6.048651725287175E-5 40-41 0.0 2.41946069011487E-4 0.0 0.15505718697773674 6.048651725287175E-5 42-43 0.0 2.41946069011487E-4 0.0 0.20117815638305142 6.048651725287175E-5 44-45 0.0 2.41946069011487E-4 0.0 0.259154483169929 6.048651725287175E-5 46-47 0.0 2.41946069011487E-4 0.0 0.32789741002781775 6.048651725287175E-5 48-49 0.0 2.41946069011487E-4 0.0 0.40011831162774664 6.048651725287175E-5 50-51 0.0 2.41946069011487E-4 0.0 0.47866005428060054 6.048651725287175E-5 52-53 0.0 2.41946069011487E-4 0.0 0.5577461755887304 9.072977587930762E-5 54-55 0.0 2.41946069011487E-4 0.0 0.6414595154667049 1.209730345057435E-4 56-57 0.0 2.41946069011487E-4 0.0 0.7414739717443284 1.209730345057435E-4 58-59 0.0 2.41946069011487E-4 0.0 0.8629611416467212 1.209730345057435E-4 60-61 0.0 2.41946069011487E-4 0.0 0.9916159638435795 1.209730345057435E-4 62-63 0.0 2.41946069011487E-4 0.0 1.1258355456277018 1.209730345057435E-4 64-65 0.0 2.41946069011487E-4 0.0 1.267736915102939 1.209730345057435E-4 66-67 0.0 2.41946069011487E-4 0.0 1.4284798347024457 1.209730345057435E-4 68-69 0.0 2.41946069011487E-4 0.0 1.6083062504952332 1.209730345057435E-4 70-71 0.0 2.41946069011487E-4 0.0 1.8055225399982215 1.209730345057435E-4 72-73 0.0 2.41946069011487E-4 0.0 2.0151083222794224 1.209730345057435E-4 74-75 0.0 2.41946069011487E-4 0.0 2.2213975893703415 1.209730345057435E-4 76-77 0.0 2.41946069011487E-4 0.0 2.4541799510180184 1.209730345057435E-4 78-79 0.0 2.41946069011487E-4 0.0 2.711943244291131 1.209730345057435E-4 80-81 0.0 2.41946069011487E-4 0.0 2.9992542012422723 1.209730345057435E-4 82-83 0.0 2.41946069011487E-4 0.0 3.2985414886094815 1.209730345057435E-4 84-85 0.0 2.41946069011487E-4 0.0 3.6010648046497193 1.209730345057435E-4 86-87 0.0 2.41946069011487E-4 0.0 3.9194658314688366 1.209730345057435E-4 88-89 0.0 2.41946069011487E-4 0.0 4.262999006206521 1.209730345057435E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2040 0.0 55.180767 2 GTACCTG 2130 0.0 53.10751 1 ACCTGGG 2140 0.0 53.04612 3 CTGGGGG 1620 0.0 50.13611 5 CCTGGGG 2165 0.0 44.535637 4 GTACCCG 215 0.0 35.370415 1 TGGGGGG 3925 0.0 31.2212 6 TATCACG 95 3.493081E-5 29.998339 2 GGTATCA 3505 0.0 29.832748 1 GTACTAG 1190 0.0 29.156439 1 GTATCAA 5090 0.0 28.293291 1 TAGGCAT 1570 0.0 27.530323 5 GCTATTG 1775 0.0 26.49229 8 CTATTGA 1775 0.0 25.957094 9 GGGGGGA 4135 0.0 25.845003 7 TAGTACT 1805 0.0 25.788046 4 CTGTGCG 1805 0.0 25.525675 9 AGGGCTA 1895 0.0 25.31522 5 TATTCGC 195 1.571243E-8 24.358362 9 ACTGTGC 2265 0.0 24.11639 8 >>END_MODULE