##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931072_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5172312
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.544814581950973	33.0	33.0	33.0	27.0	33.0
2	31.487739912054803	33.0	33.0	33.0	27.0	33.0
3	31.49295170128948	33.0	33.0	33.0	27.0	33.0
4	35.17324128938858	37.0	37.0	37.0	33.0	37.0
5	35.17463815021213	37.0	37.0	37.0	33.0	37.0
6	35.265393502944136	37.0	37.0	37.0	33.0	37.0
7	35.23384474873132	37.0	37.0	37.0	33.0	37.0
8	35.23907451831985	37.0	37.0	37.0	33.0	37.0
9	35.24296272150636	37.0	37.0	37.0	33.0	37.0
10-11	35.17019187937619	37.0	37.0	37.0	33.0	37.0
12-13	35.099780813686415	37.0	37.0	37.0	33.0	37.0
14-15	36.8809251066061	40.0	37.0	40.0	33.0	40.0
16-17	36.85173129540523	40.0	37.0	40.0	33.0	40.0
18-19	36.7899641398276	40.0	37.0	40.0	33.0	40.0
20-21	36.71580726375362	40.0	37.0	40.0	33.0	40.0
22-23	36.648224237052986	40.0	37.0	40.0	33.0	40.0
24-25	36.53657445645197	40.0	37.0	40.0	33.0	40.0
26-27	36.36752983965391	40.0	37.0	40.0	33.0	40.0
28-29	36.289266482764376	40.0	37.0	40.0	33.0	40.0
30-31	36.19056729369767	40.0	37.0	40.0	33.0	40.0
32-33	36.04610916742841	40.0	37.0	40.0	33.0	40.0
34-35	35.962069476860634	40.0	37.0	40.0	33.0	40.0
36-37	35.78381340878122	40.0	37.0	40.0	30.0	40.0
38-39	35.63065143788697	38.5	37.0	40.0	27.0	40.0
40-41	35.42963185515491	37.0	37.0	40.0	27.0	40.0
42-43	35.283882140133855	37.0	37.0	40.0	27.0	40.0
44-45	35.05674570675551	37.0	37.0	40.0	27.0	40.0
46-47	34.939650198982584	37.0	37.0	40.0	27.0	40.0
48-49	34.96485788173645	37.0	37.0	40.0	27.0	40.0
50-51	34.17179483758907	37.0	35.0	38.5	24.5	40.0
52-53	34.288795416827135	37.0	35.0	38.5	27.0	40.0
54-55	34.83634436592379	37.0	37.0	40.0	27.0	40.0
56-57	34.805435460969875	37.0	37.0	40.0	27.0	40.0
58-59	34.68191072000297	37.0	37.0	40.0	27.0	40.0
60-61	34.71349814164343	37.0	37.0	40.0	27.0	40.0
62-63	34.577918056760694	37.0	35.0	40.0	27.0	40.0
64-65	34.394961962851426	37.0	33.0	40.0	27.0	40.0
66-67	34.19868233006826	37.0	33.0	40.0	24.5	40.0
68-69	34.00632212441941	37.0	33.0	40.0	22.0	40.0
70-71	33.788429429624514	37.0	33.0	40.0	22.0	40.0
72-73	33.182893259339345	37.0	33.0	40.0	22.0	40.0
74-75	32.9843263515426	37.0	33.0	37.0	22.0	40.0
76-77	32.614035464217935	37.0	33.0	37.0	15.0	40.0
78-79	32.34910655041691	37.0	33.0	37.0	15.0	38.5
80-81	32.058829494431116	37.0	33.0	37.0	15.0	37.0
82-83	31.800243778797565	37.0	33.0	37.0	15.0	37.0
84-85	31.436103139176446	37.0	33.0	37.0	15.0	37.0
86-87	31.28108223169832	37.0	33.0	37.0	6.0	37.0
88-89	31.08799372504984	37.0	33.0	37.0	6.0	37.0
90-91	30.817493994948485	37.0	33.0	37.0	2.0	37.0
92-93	30.55174040158444	35.0	33.0	37.0	2.0	37.0
94-95	30.33274075500473	33.0	33.0	37.0	2.0	37.0
96-97	30.054607881349774	33.0	33.0	37.0	2.0	37.0
98-99	29.748486846887815	33.0	33.0	37.0	2.0	37.0
100-101	29.05068130074133	33.0	30.0	37.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.07402207306739328
1101	2	-0.037284595405274956
1101	3	-0.09789175696253949
1101	4	-0.08305241392024953
1101	5	-0.10004473523532198
1101	6	-0.1405633823719299
1101	7	0.010011085747372306
1101	8	-0.06209229082158885
1101	9	0.009622387178040981
1101	10-11	-0.018497950828511023
1101	12-13	0.012159377213855294
1101	14-15	0.10658771954455659
1101	16-17	0.016679093465860717
1101	18-19	0.10443575622497292
1101	20-21	0.1295526573125514
1101	22-23	0.11520935859296344
1101	24-25	0.13254602400380833
1101	26-27	0.11665149823273424
1101	28-29	0.07390852409317006
1101	30-31	0.09542021638808507
1101	32-33	0.12191778116995522
1101	34-35	0.17586740562262548
1101	36-37	0.1107967104861487
1101	38-39	0.10924778069771435
1101	40-41	0.09672100278319107
1101	42-43	0.06713983476964813
1101	44-45	0.021156719795406786
1101	46-47	-0.019833831425572157
1101	48-49	0.08686407604680113
1101	50-51	0.03732174993159987
1101	52-53	0.18007102034540168
1101	54-55	0.2595418043720841
1101	56-57	0.23892219569696493
1101	58-59	0.23626586687490914
1101	60-61	0.18841911698347502
1101	62-63	0.2261510062891432
1101	64-65	0.2587996470944489
1101	66-67	0.21427316317208067
1101	68-69	0.14788749403268753
1101	70-71	0.08581877706935614
1101	72-73	0.14026476384960773
1101	74-75	0.1315707488169764
1101	76-77	-0.04388845267521191
1101	78-79	0.04022343117442517
1101	80-81	0.12335035864528976
1101	82-83	0.07105703747884462
1101	84-85	0.11331479057950844
1101	86-87	0.134220041436528
1101	88-89	0.12751899719133775
1101	90-91	0.05197303851475965
1101	92-93	0.04028972549839338
1101	94-95	-0.06984673965363086
1101	96-97	-0.17504556436056973
1101	98-99	-0.08066701500891327
1101	100-101	-0.010663429157872883
1102	1	0.17520661167100826
1102	2	0.183431878950163
1102	3	0.18206600817851992
1102	4	0.21552549513217656
1102	5	0.12250513686390718
1102	6	0.10305768168887397
1102	7	0.14537265846678338
1102	8	0.1529020042519278
1102	9	0.08454463455101546
1102	10-11	0.15553175159516286
1102	12-13	0.19380975723556304
1102	14-15	0.24695881581059353
1102	16-17	0.32780703564367286
1102	18-19	0.2518249472295935
1102	20-21	0.2527931015606981
1102	22-23	0.3002089675294499
1102	24-25	0.28935202429826035
1102	26-27	0.24386043196357576
1102	28-29	0.19790705645483087
1102	30-31	0.22716560183868495
1102	32-33	0.2753523539445766
1102	34-35	0.16758778601239754
1102	36-37	0.2638719649074588
1102	38-39	0.26130983557543175
1102	40-41	0.26147076814508097
1102	42-43	0.3431560247989154
1102	44-45	0.24108244073249097
1102	46-47	0.244897185891638
1102	48-49	0.21355853122631885
1102	50-51	0.15650962604268415
1102	52-53	0.15870181498645053
1102	54-55	0.22056461947725126
1102	56-57	0.3649037651038327
1102	58-59	0.3084092629624351
1102	60-61	0.3305383821521346
1102	62-63	0.30953092218747713
1102	64-65	0.2492545919774969
1102	66-67	0.2577590802850196
1102	68-69	0.2326033518880024
1102	70-71	0.3095792207654142
1102	72-73	0.1226627652850425
1102	74-75	0.15220475477955375
1102	76-77	0.06454621211435096
1102	78-79	0.12854707993498948
1102	80-81	0.21427434970144077
1102	82-83	0.15805337068335845
1102	84-85	0.21869777977695293
1102	86-87	0.2171100927808638
1102	88-89	0.21970198270818386
1102	90-91	0.23775914517847951
1102	92-93	0.25396314606189563
1102	94-95	0.1613815218160326
1102	96-97	0.18013970772761922
1102	98-99	0.20369711572591953
1102	100-101	0.2219015793995176
1103	1	0.0981202011654112
1103	2	0.0222020128663587
1103	3	0.08041959676073418
1103	4	0.0709494169085616
1103	5	-0.013478087650092618
1103	6	0.02426964608547877
1103	7	0.03564246434618212
1103	8	0.11142964529897625
1103	9	0.050881393266791974
1103	10-11	0.08789547598811254
1103	12-13	0.08290104268371579
1103	14-15	0.19335102502439128
1103	16-17	0.12734192946150102
1103	18-19	0.21342299953303723
1103	20-21	0.1683285821609175
1103	22-23	0.1227724391411158
1103	24-25	0.161013613996154
1103	26-27	0.21402050778042536
1103	28-29	0.1480307500088216
1103	30-31	0.18213092992810687
1103	32-33	0.20982509619185663
1103	34-35	0.24934839195073977
1103	36-37	0.19860810140585272
1103	38-39	0.15354887090165903
1103	40-41	0.19410091279703323
1103	42-43	0.13836280638955856
1103	44-45	0.10705605657508244
1103	46-47	0.08238799823003262
1103	48-49	0.051742189731243116
1103	50-51	0.14787243473934097
1103	52-53	0.26985986820209007
1103	54-55	0.381574793003459
1103	56-57	0.4002805600966326
1103	58-59	0.30970246470682383
1103	60-61	0.31433500898659616
1103	62-63	0.3720646687536231
1103	64-65	0.4261789794959583
1103	66-67	0.4590920367561182
1103	68-69	0.30528779803207584
1103	70-71	0.3856275132198661
1103	72-73	0.19009528969483824
1103	74-75	0.29527293819246125
1103	76-77	0.28720138492132463
1103	78-79	0.2962530633612346
1103	80-81	0.2554311048035487
1103	82-83	0.31640407797980785
1103	84-85	0.363586391988342
1103	86-87	0.26673824465313345
1103	88-89	0.2858972865193756
1103	90-91	0.4455467080229596
1103	92-93	0.2796833359373103
1103	94-95	0.29703618327153336
1103	96-97	0.11020230662106556
1103	98-99	0.2197171084284193
1103	100-101	0.28174654254361897
1104	1	-1.2258524193242692E-4
1104	2	0.024259418710034453
1104	3	0.00952830499604218
1104	4	0.049038507914005436
1104	5	0.004371827918653537
1104	6	0.020677534483780846
1104	7	0.0351234701851979
1104	8	0.025854079992086554
1104	9	0.03460077208628576
1104	10-11	-2.3254493240898455E-4
1104	12-13	0.017342679455573773
1104	14-15	0.09349539961399245
1104	16-17	0.04939387771344883
1104	18-19	0.051424082239520885
1104	20-21	0.06011327600850791
1104	22-23	0.041434042800950976
1104	24-25	0.08140040794184245
1104	26-27	0.1380478579985791
1104	28-29	0.10846321000531844
1104	30-31	0.06487944707441073
1104	32-33	0.08303664268368749
1104	34-35	0.06771546518891114
1104	36-37	0.13488301793298518
1104	38-39	0.1084387293793938
1104	40-41	0.1826408143026086
1104	42-43	0.13642497838176837
1104	44-45	0.11749968169906566
1104	46-47	-0.02401848726989897
1104	48-49	-0.07016175212493891
1104	50-51	-0.017150743554715575
1104	52-53	0.06887229692635088
1104	54-55	0.15485266319372926
1104	56-57	0.24518166276156705
1104	58-59	0.13870937119155968
1104	60-61	0.12949787799323786
1104	62-63	0.13625375870081768
1104	64-65	0.17357598948181874
1104	66-67	0.1483863245220718
1104	68-69	0.23261506828049505
1104	70-71	0.31042244279020537
1104	72-73	0.21832694144563902
1104	74-75	0.16835063903845082
1104	76-77	0.14206306601852248
1104	78-79	0.06385335402102754
1104	80-81	0.05976596194447836
1104	82-83	0.11054169222162713
1104	84-85	0.2538507714186977
1104	86-87	0.10413073217559088
1104	88-89	0.11610802145488464
1104	90-91	0.1680881018632263
1104	92-93	0.07843809995494055
1104	94-95	0.028362409766859997
1104	96-97	-0.011440799904054444
1104	98-99	0.05877971207849697
1104	100-101	0.19232181858250286
1105	1	0.054879471972093086
1105	2	0.013302128450110473
1105	3	-0.05551031564504072
1105	4	0.07325394023166609
1105	5	0.02815902148334004
1105	6	-0.04736933694466927
1105	7	0.08078296481481573
1105	8	0.042727906978534236
1105	9	0.03554605575324388
1105	10-11	-0.0033339842717694523
1105	12-13	0.09703180692500979
1105	14-15	0.08622873896040062
1105	16-17	0.06832314260230277
1105	18-19	0.060380782054309634
1105	20-21	0.07249353055322416
1105	22-23	0.07754967444596872
1105	24-25	0.03735378764177
1105	26-27	0.0771313683779411
1105	28-29	0.1013995293467076
1105	30-31	0.06383097450949293
1105	32-33	0.08821456312448817
1105	34-35	0.11669131006756572
1105	36-37	0.16085125220086383
1105	38-39	0.11886227530592919
1105	40-41	0.08740935378568082
1105	42-43	0.05498523050477644
1105	44-45	0.04336425987134618
1105	46-47	-0.039522884642103406
1105	48-49	-0.030216142423533654
1105	50-51	-0.034030825583805324
1105	52-53	-0.01739533997722731
1105	54-55	0.12989125912353217
1105	56-57	0.09615409408583275
1105	58-59	0.11364435349828028
1105	60-61	0.023991969371842004
1105	62-63	0.12063538055151923
1105	64-65	0.1788774470148624
1105	66-67	0.17521223324848734
1105	68-69	0.23534410091421876
1105	70-71	0.18020705001060122
1105	72-73	0.15246425754737913
1105	74-75	0.1662920929204077
1105	76-77	0.12704719906034967
1105	78-79	0.23484017657673917
1105	80-81	0.33115770584419124
1105	82-83	0.31344768850628313
1105	84-85	0.40276847142870764
1105	86-87	0.4088121798426627
1105	88-89	0.38760156424308434
1105	90-91	0.35695886356988993
1105	92-93	0.33536480676302105
1105	94-95	0.2959924019086735
1105	96-97	0.2554584912837825
1105	98-99	0.24693423279087057
1105	100-101	0.25963285374418277
1106	1	0.03641167418174618
1106	2	0.04670774653722276
1106	3	0.005940444301675285
1106	4	-0.013759295100584268
1106	5	-0.005119396116363362
1106	6	-0.04514844035445975
1106	7	0.016218204218716892
1106	8	0.0317991975854639
1106	9	0.06120459702098913
1106	10-11	0.10308303533091134
1106	12-13	0.05809121221469837
1106	14-15	0.1309634952725034
1106	16-17	0.07648685213734296
1106	18-19	0.0829384208120203
1106	20-21	0.11325202740689377
1106	22-23	0.06690694431069488
1106	24-25	0.0317932320306582
1106	26-27	0.001966972261193689
1106	28-29	0.10972010790869291
1106	30-31	0.10908520032804603
1106	32-33	0.05737527370646234
1106	34-35	0.020167533105841073
1106	36-37	0.09507328368022172
1106	38-39	0.013616719336781102
1106	40-41	0.13244239659108814
1106	42-43	0.18169182739906375
1106	44-45	0.18512713902679678
1106	46-47	0.04679126884849438
1106	48-49	0.06664953648523664
1106	50-51	0.004173264029539325
1106	52-53	0.07621667623653394
1106	54-55	0.10624061053608358
1106	56-57	0.14186596387821737
1106	58-59	0.23404860413192807
1106	60-61	0.03807308647684948
1106	62-63	0.1645894954737841
1106	64-65	0.22120588800989083
1106	66-67	0.24489807698008548
1106	68-69	0.25358066542542446
1106	70-71	0.26371685135406864
1106	72-73	0.13003308624423937
1106	74-75	0.16105439969295077
1106	76-77	0.052694038809185884
1106	78-79	0.1821800428530338
1106	80-81	0.2583337871216429
1106	82-83	0.2604752827943706
1106	84-85	0.35516088227030806
1106	86-87	0.36837382543502173
1106	88-89	0.3105281191369045
1106	90-91	0.34161264753168297
1106	92-93	0.3496394445367734
1106	94-95	0.37232793372322703
1106	96-97	0.3892274737477841
1106	98-99	0.3896650046451313
1106	100-101	0.495535725658808
1107	1	-0.1807656238995783
1107	2	-0.1485722800562641
1107	3	-0.18409854462365516
1107	4	-0.0729538400468428
1107	5	-0.15724117614512068
1107	6	-0.16091250305596105
1107	7	-0.2212078337313983
1107	8	-0.23057768745765372
1107	9	-0.19755164109981393
1107	10-11	-0.22057729094978384
1107	12-13	-0.1978309963770144
1107	14-15	-0.22876199596297653
1107	16-17	-0.21573819180731846
1107	18-19	-0.19526512401451868
1107	20-21	-0.1558156774616677
1107	22-23	-0.21474696606905042
1107	24-25	-0.17067700581461764
1107	26-27	-0.14053691901440146
1107	28-29	-0.25735353250264126
1107	30-31	-0.13881436889480625
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2311	24-25	-0.16248997037886426
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2312	80-81	-0.294481841539433
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2312	84-85	-1.3542936887075392
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2312	98-99	-0.33573089001701817
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2313	1	-0.1517687611660925
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2313	24-25	-0.407368871543369
2313	26-27	-1.608787135560135
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2313	80-81	-0.31598621217255385
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2313	86-87	-0.5006461359220609
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2313	98-99	-0.4436987406561954
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2314	4	-0.2749393859447551
2314	5	-0.2513633502672903
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2314	24-25	-0.595605341259386
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2314	82-83	-0.5563283863090369
2314	84-85	-0.570085700030539
2314	86-87	-0.6965055520541767
2314	88-89	-0.6546890161060723
2314	90-91	-0.6923199334293173
2314	92-93	-0.6708691061526544
2314	94-95	-0.6392055954330438
2314	96-97	-0.5974216719194487
2314	98-99	-0.6661283359027337
2314	100-101	-0.7332877907616044
2315	1	-0.3234622140939223
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2315	3	-0.39050119202120825
2315	4	-0.3168131272984027
2315	5	-0.36733958837118763
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2315	7	-0.4035312639721198
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2315	9	-0.364422049684336
2315	10-11	-0.47294199247540547
2315	12-13	-0.43990546371369277
2315	14-15	-0.7699189061789227
2315	16-17	-0.6058414358811959
2315	18-19	-0.5739370364592844
2315	20-21	-0.6300313179793733
2315	22-23	-0.5741157363458242
2315	24-25	-0.6807648652132414
2315	26-27	-0.673046243672367
2315	28-29	-0.5914426827889798
2315	30-31	-0.6217910266392224
2315	32-33	-0.5672228561860848
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2315	84-85	-0.5232345553779751
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2315	88-89	-0.5926033170227356
2315	90-91	-0.6644751400998921
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2315	96-97	-0.49227804311151857
2315	98-99	-0.46492441330401135
2315	100-101	-0.517923381070954
2316	1	-0.43198459847738135
2316	2	-0.93350199080637
2316	3	-0.5836600560309044
2316	4	-0.49028087740163073
2316	5	-0.4298281644040216
2316	6	-0.5419899199222584
2316	7	-0.6657137226315939
2316	8	-0.5912366982783439
2316	9	-0.5724537952785056
2316	10-11	-0.4430603702450995
2316	12-13	-0.543667357374467
2316	14-15	-0.7635686187294297
2316	16-17	-0.7640184194264847
2316	18-19	-0.7483444434449638
2316	20-21	-0.9506950197582498
2316	22-23	-0.7727337134901049
2316	24-25	-0.7643319416544827
2316	26-27	-0.7745060244160911
2316	28-29	-0.7090623205660691
2316	30-31	-0.6596203739943434
2316	32-33	-0.7433099833752124
2316	34-35	-0.7020088182611914
2316	36-37	-0.7429779426200511
2316	38-39	-0.6927580230968147
2316	40-41	-0.6403399704556634
2316	42-43	-0.6076559410793578
2316	44-45	-0.6796132828802683
2316	46-47	-0.5577017022663
2316	48-49	-0.6437862049788308
2316	50-51	-0.5763199701193997
2316	52-53	-0.6643955557537637
2316	54-55	-0.6243146775456268
2316	56-57	-0.608093569330066
2316	58-59	-0.5853165716173123
2316	60-61	-0.6792848417864477
2316	62-63	-0.7260752923983489
2316	64-65	-0.7146829596116078
2316	66-67	-0.6707494051521152
2316	68-69	-0.6584781728530515
2316	70-71	-0.6238958597601325
2316	72-73	-0.35757372224979633
2316	74-75	-0.45915349699387065
2316	76-77	-0.5062926421576961
2316	78-79	-0.5086849282696164
2316	80-81	-0.5645380071679007
2316	82-83	-0.49311017684687997
2316	84-85	-0.4698695715378314
2316	86-87	-0.6448144366334532
2316	88-89	-0.5561517974927739
2316	90-91	-0.5439740856489941
2316	92-93	-0.500146914821002
2316	94-95	-0.5530459710623461
2316	96-97	-0.5108683510327516
2316	98-99	-0.49488655015669636
2316	100-101	-0.5733506719022365
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107662.0
3	16872.0
4	13134.0
5	11311.0
6	11765.0
7	12256.0
8	12467.0
9	12745.0
10	13140.0
11	13272.0
12	13374.0
13	14202.0
14	14842.0
15	16611.0
16	19510.0
17	24003.0
18	21873.0
19	17354.0
20	17855.0
21	19401.0
22	21565.0
23	24791.0
24	29376.0
25	34468.0
26	38510.0
27	46128.0
28	55567.0
29	67611.0
30	84226.0
31	108082.0
32	140690.0
33	188022.0
34	263605.0
35	390337.0
36	624170.0
37	1202624.0
38	1448278.0
39	613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.32504945579294	25.395917016350182	13.483934407466238	22.795099120390635
2	13.428775876493127	22.06220392926146	44.58719708003512	19.921823114210294
3	16.65984185777098	32.51971171079205	30.032909885842017	20.787536545594943
4	10.381563976323719	19.090108492120958	44.744091805040924	25.7842357265144
5	11.480406579687912	39.238752559103716	37.213239728155465	12.0676011330529
6	23.93857911123691	43.34523903430419	21.261575094464526	11.45460675999437
7	22.79433259246542	32.77281030224008	29.032181353329033	15.400675751965466
8	21.818637390783852	38.88311455302774	22.175847087337345	17.122400968851068
9	25.31780758778666	15.680743930373882	24.884751731914083	34.116696749925374
10-11	21.883729479786442	28.347506727411947	31.250823495930298	18.51794029687131
12-13	22.44296719733558	25.999261258295014	30.72008369183702	20.837687852532387
14-15	20.494354941306625	27.408425613102832	27.871648337030713	24.225571108559834
16-17	18.72787396313355	29.474462275675094	30.12965398406906	21.66800977712229
18-19	18.329089582220192	31.07774392852285	31.10731597299325	19.485850516263707
20-21	19.183461863862814	28.95840390138878	30.392076116057964	21.46605811869044
22-23	19.54416129576097	30.456070708804884	29.424298070186023	20.57546992524813
24-25	19.61332955294746	29.936489710644842	30.105824059513818	20.34435667689388
26-27	19.91994102443936	29.268129996798336	29.577923760206264	21.234005218556035
28-29	19.365324442918368	30.995481324405798	28.82421826061537	20.814975972060463
30-31	20.993223146631525	29.78517150550856	28.613886787958652	20.607718559901258
32-33	20.6698764498352	30.024242543759925	28.87230120688775	20.43357979951712
34-35	21.230699153492676	28.67898340239336	29.83104074154846	20.259276702565508
36-37	21.324960675226087	29.55666634186028	29.633392185158204	19.484980797755433
38-39	22.147861536581708	28.50597566426774	30.650094193853732	18.69606860529682
40-41	20.516656033099395	28.879415347137638	30.792925777700443	19.811002842062525
42-43	21.36760324663354	28.843148754671873	29.591083212989012	20.19816478570558
44-45	20.44540275719532	30.24576178613514	29.52091773751833	19.787917719151206
46-47	21.958517689203855	28.95141925777723	28.43926443078932	20.650798622229598
48-49	21.655726476323164	27.632215238883067	29.899897841453665	20.8121604433401
50-51	21.524612576444902	28.515800513571897	28.285183339149157	21.67440357083405
52-53	19.97248395147762	28.91337605308349	29.463522417004423	21.650617578434463
54-55	20.754204187266442	29.84502774598219	28.33363773682487	21.067130329926503
56-57	21.3970238170269	28.344715175973338	29.294138294478632	20.964122712521128
58-59	20.96864932204399	27.737941043745273	30.756847094880097	20.536562539330642
60-61	20.84888011773969	30.390228893258953	28.368705842527625	20.392185146473732
62-63	21.5497677246398	29.161356250806982	27.31143160798294	21.97744441657028
64-65	19.836682533014287	29.217191255175884	28.237555922041714	22.708570289768115
66-67	20.980978006456	27.087669267403292	29.525639363099625	22.405713363041084
68-69	19.526329243007012	28.575795906288704	28.577540071716207	23.320334778988077
70-71	20.0001647502338	28.32085592592054	29.44989896934192	22.22908035450374
72-73	21.0477784052267	27.96589982965314	29.797562279720108	21.188759485400052
74-75	21.049674968797277	29.27570611651129	28.1245826921249	21.550036222566533
76-77	19.985241891431105	29.2695806391516	29.772689132652243	20.972488336765053
78-79	20.040848214047514	28.717053115580963	29.04716830762219	22.194930362749332
80-81	20.09187890974084	29.149629427936652	30.059022347582165	20.699469314740345
82-83	21.362737041878674	27.741185421708753	30.1311391621628	20.76493837424977
84-85	22.345816055190788	27.775469016930277	28.77086645613482	21.107848471744113
86-87	20.840586904215844	29.24843298155998	28.901637750750332	21.00934236347385
88-89	21.661874322374082	27.714503886484778	29.309676607134598	21.313945184006542
90-91	21.374493438569523	27.031494284736713	30.184518726658922	21.409493550034846
92-93	20.79545158384701	28.5661663154413	29.193634286855875	21.44474781385582
94-95	19.75170773813029	27.782619425443748	30.061527774764528	22.404145061661435
96-97	20.58280063246409	27.766672178589396	28.86726889908573	22.783258289860786
98-99	19.355782875148332	28.80395085807057	29.549505203572945	22.29076106320815
100-101	20.282768283115665	26.96905090094377	29.23088153952847	23.51729927641209
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	103.0
1	67.0
2	45.0
3	60.0
4	52.0
5	52.0
6	76.5
7	139.0
8	233.5
9	262.0
10	225.5
11	242.0
12	368.5
13	621.5
14	798.0
15	1011.5
16	1397.0
17	1647.0
18	1780.5
19	1918.5
20	2093.0
21	2354.0
22	2648.0
23	2926.0
24	3163.0
25	3524.0
26	4337.5
27	6084.5
28	11562.5
29	28058.5
30	52693.0
31	81124.0
32	119762.0
33	177580.5
34	263456.0
35	325546.0
36	371280.5
37	435042.5
38	426916.0
39	331886.5
40	242561.5
41	182151.5
42	144947.0
43	125636.0
44	109458.0
45	106450.5
46	111550.5
47	120909.5
48	132395.5
49	129257.0
50	117927.0
51	115759.0
52	113472.0
53	109701.0
54	105359.5
55	93642.5
56	83259.0
57	69528.0
58	55307.0
59	51526.0
60	44363.5
61	33355.0
62	25534.0
63	21212.5
64	16760.0
65	10860.0
66	7404.0
67	5543.0
68	4407.0
69	3535.0
70	2857.5
71	2305.5
72	1846.0
73	1541.5
74	1304.0
75	1066.5
76	843.0
77	656.5
78	529.0
79	437.0
80	383.5
81	319.5
82	255.5
83	219.5
84	164.5
85	129.0
86	123.5
87	113.0
88	96.0
89	70.0
90	46.5
91	36.5
92	24.5
93	23.5
94	19.5
95	15.5
96	12.5
97	6.0
98	3.5
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.093394211331412E-4
2	0.0037700741950601586
3	1.1600228292492797E-4
4	9.086845495786023E-4
5	0.0017593679576947407
6	0.0
7	0.0
8	0.0
9	0.0
10-11	6.766799837287465E-5
12-13	6.766799837287465E-5
14-15	0.003441401060106196
16-17	0.004601423889355476
18-19	0.003818408479612212
20-21	0.0
22-23	0.0
24-25	9.666856910410663E-6
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	2.3200456584985594E-4
42-43	9.666856910410663E-6
44-45	5.220102731621759E-4
46-47	0.7389345422317911
48-49	0.5291250798482381
50-51	0.4086083747461483
52-53	0.34540646426588345
54-55	0.33682229532943875
56-57	0.3036746429836406
58-59	0.3037133104112822
60-61	0.2802035144051635
62-63	0.27473207339387107
64-65	0.2709619991988109
66-67	0.25885909434697674
68-69	0.23685732801888207
70-71	0.25108694139100657
72-73	0.8157087198142726
74-75	0.5361045505375546
76-77	0.6334594664823003
78-79	0.3548413166104442
80-81	0.3085660725803084
82-83	0.28787899879202955
84-85	0.2788404875807956
86-87	0.252720640208866
88-89	0.25550469499906425
90-91	0.26597390103303903
92-93	0.2237007357638132
94-95	0.19194511081311413
96-97	0.1498362821113653
98-99	0.1579177744884686
100-101	0.15423470200560213
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5172312.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.42062959244891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.08364025388633	17.50684375362419
2	10.588512118988568	4.748022163099981
3	3.965058190777462	2.6669730302378127
4	1.9382818439751786	1.7382999707814535
5	1.1430116960139298	1.2813520928082567
6	0.7220010059036817	0.9712630271245181
7	0.48917983439733803	0.7677403909782751
8	0.34702656339156635	0.6224443229234241
9	0.27344578876458264	0.5517744069155163
>10	1.7812056071391993	7.8098644626378775
>50	0.25544252338820705	4.0362314829319486
>100	0.3097959405942516	15.03108188051474
>500	0.05820510284829883	8.936143237110015
>1k	0.03860099965348453	16.617215749196788
>5k	0.004076959513963535	6.1303455311708985
>10k+	0.0025155707639349465	10.584404497944362
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	38252	0.7395532210740574	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	34479	0.6666071188280985	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	32037	0.6193941896776528	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	27884	0.5391012761797819	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	25934	0.5014005342291803	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	25701	0.4968957789089289	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	25440	0.49184967960169457	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	23555	0.45540562904944637	No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG	21876	0.4229443235442874	No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA	20527	0.3968631435999994	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	20480	0.39595445905042076	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	20391	0.3942337585203677	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	19252	0.3722126584784522	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	19060	0.36850058542485453	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	17298	0.3344345816725673	No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT	15664	0.3028432932893453	No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA	14935	0.2887490159139665	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	14318	0.2768201144865198	No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA	13810	0.26699858786554254	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	13393	0.25893642920226	No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG	12056	0.2330872538238219	No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA	11610	0.2244644174597356	No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG	11522	0.22276305064350332	No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA	11448	0.22133235582076255	No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA	11120	0.21499089768753318	No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG	11059	0.21381154114446305	No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT	10548	0.20393201338202332	No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC	10408	0.20122529344710838	No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG	10170	0.1966238695577529	No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC	9788	0.18923839087819916	No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG	9694	0.18742102177904194	No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA	8902	0.17210872043295145	No Hit
GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT	8767	0.1694986690671406	No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT	8690	0.16800997310293733	No Hit
ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT	8252	0.1595418064494176	No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA	8240	0.15930980188356775	No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA	7999	0.1546503768527498	No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA	7921	0.15314234717472572	No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG	7843	0.15163431749670167	No Hit
AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA	7814	0.15107363979589783	No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT	7813	0.15105430608207704	No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG	7791	0.15062896437801895	No Hit
GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG	7484	0.14469351423502683	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	7445	0.14393949939601478	No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT	7344	0.14198679430011182	No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT	6869	0.1328032802352217	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	6825	0.13195259682710556	No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA	6785	0.13117924827427271	No Hit
TTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGC	6666	0.12887853632959498	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	6629	0.12816318891822456	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	6560	0.1268291626645879	No Hit
ATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAA	6540	0.1264424883881715	No Hit
GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT	6503	0.1257271409768011	No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA	6420	0.12412244272967292	No Hit
CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC	6385	0.12344576274594418	No Hit
GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG	6130	0.11851566572163473	No Hit
ATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAA	6024	0.11646629205662767	No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA	5977	0.11555760750704908	No Hit
GTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGA	5959	0.11520960065827428	No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA	5861	0.1133148967038338	No Hit
CTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCAC	5857	0.11323756184855051	No Hit
ACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTC	5841	0.11292822242741737	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	5721	0.1106081767689188	No Hit
CAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAAT	5698	0.11016350135103993	No Hit
ATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATT	5661	0.10944815393966953	No Hit
GGAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCT	5650	0.1092354830876405	No Hit
TCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCA	5545	0.10720544313645426	No Hit
GCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACTCCACAAAT	5512	0.10656743058036716	No Hit
ATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTC	5482	0.10598741916574252	No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT	5408	0.10455672434300173	No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT	5242	0.1013473278487454	No Hit
CTCTCACAGCCAATAACTTCAACAACTTCCCTATCTTTAATCCTCTCACT	5227	0.10105732214143308	No Hit
CCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGG	5177	0.10009063645039201	No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA	5176	0.1000713027365712	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.9333713820821326E-5	0.0
5	0.0	0.0	0.0	1.9333713820821326E-5	0.0
6	0.0	0.0	0.0	1.9333713820821326E-5	0.0
7	0.0	0.0	0.0	1.9333713820821326E-5	0.0
8	0.0	0.0	0.0	1.9333713820821326E-5	0.0
9	0.0	0.0	0.0	1.9333713820821326E-5	0.0
10-11	0.0	0.0	0.0	3.866742764164265E-5	0.0
12-13	0.0	0.0	0.0	3.866742764164265E-5	0.0
14-15	0.0	0.0	0.0	5.800114146246398E-5	0.0
16-17	0.0	0.0	0.0	5.800114146246398E-5	0.0
18-19	0.0	0.0	0.0	5.800114146246398E-5	0.0
20-21	0.0	0.0	0.0	1.2566913983533864E-4	0.0
22-23	0.0	0.0	0.0	2.126708520290346E-4	9.666856910410663E-6
24-25	0.0	0.0	0.0	3.770074195060159E-4	1.9333713820821326E-5
26-27	0.0	0.0	0.0	8.990176926681917E-4	1.9333713820821326E-5
28-29	0.0	0.0	0.0	0.0024747153690651297	1.9333713820821326E-5
30-31	0.0	0.0	0.0	0.011000883164047336	1.9333713820821326E-5
32-33	0.0	0.0	0.0	0.039846784184712755	1.9333713820821326E-5
34-35	0.0	0.0	0.0	0.08751405560994774	1.9333713820821326E-5
36-37	0.0	0.0	0.0	0.16507124860217248	1.9333713820821326E-5
38-39	0.0	0.0	0.0	0.2728567031532514	1.9333713820821326E-5
40-41	0.0	0.0	0.0	0.38343587935143897	1.9333713820821326E-5
42-43	0.0	0.0	0.0	0.5126044213883463	1.9333713820821326E-5
44-45	0.0	0.0	0.0	0.6603719961208836	1.9333713820821326E-5
46-47	0.0	0.0	0.0	0.8437619385682844	1.9333713820821326E-5
48-49	0.0	0.0	0.0	1.0312312946318782	1.9333713820821326E-5
50-51	0.0	0.0	0.0	1.2250131082579707	1.9333713820821326E-5
52-53	0.0	0.0	0.0	1.4197809412889246	1.9333713820821326E-5
54-55	0.0	0.0	0.0	1.6310790996366809	1.9333713820821326E-5
56-57	0.0	0.0	0.0	1.8914365568047713	1.9333713820821326E-5
58-59	0.0	0.0	0.0	2.190519442755967	1.9333713820821326E-5
60-61	0.0	0.0	0.0	2.5031842626662892	1.9333713820821326E-5
62-63	0.0	0.0	0.0	2.8048481994125645	1.9333713820821326E-5
64-65	0.0	0.0	0.0	3.1268318693845227	1.9333713820821326E-5
66-67	0.0	0.0	0.0	3.4833068848128264	3.866742764164265E-5
68-69	0.0	0.0	0.0	3.870309834364207	3.866742764164265E-5
70-71	0.0	0.0	0.0	4.265278274009766	3.866742764164265E-5
72-73	0.0	0.0	0.0	4.728543057727376	3.866742764164265E-5
74-75	0.0	0.0	0.0	5.146083608258744	3.866742764164265E-5
76-77	0.0	0.0	0.0	5.624351353901311	3.866742764164265E-5
78-79	0.0	0.0	0.0	6.157778185074682	3.866742764164265E-5
80-81	0.0	0.0	0.0	6.773005959423948	3.866742764164265E-5
82-83	0.0	0.0	0.0	7.435756002344792	3.866742764164265E-5
84-85	0.0	0.0	0.0	8.057586239963868	3.866742764164265E-5
86-87	0.0	0.0	0.0	8.737697957895811	3.866742764164265E-5
88-89	0.0	0.0	0.0	9.458265471997823	3.866742764164265E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTGGG	3590	0.0	50.65516	3
TACCTGG	3585	0.0	50.199356	2
GTACCTG	4320	0.0	42.53551	1
CCTGGGG	3680	0.0	42.33845	4
CTGGGGG	3405	0.0	41.167366	5
TATATCC	6630	0.0	32.428677	4
TATTCGC	1360	0.0	32.385597	9
GTACACG	450	0.0	31.572752	1
TGGGGGG	10385	0.0	31.281208	6
TATTCTC	4725	0.0	29.265741	5
ATATATC	7600	0.0	28.850563	3
TCTCTGC	4875	0.0	28.36526	8
CTGTGCG	3450	0.0	27.592106	9
GTACTAG	3125	0.0	27.581953	1
CCCATTT	7965	0.0	27.173046	9
GGGGGGA	10810	0.0	26.54684	7
TAGGCAT	3675	0.0	26.416485	5
CTTAATA	2675	0.0	26.379475	2
GTACATG	14965	0.0	26.266674	1
CATGGGG	10470	0.0	26.188992	4
>>END_MODULE