##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931071_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3997492 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.269092721136154 33.0 33.0 33.0 27.0 33.0 2 31.194775374159597 33.0 33.0 33.0 27.0 33.0 3 31.210894230682637 33.0 33.0 33.0 27.0 33.0 4 34.84018404539646 37.0 37.0 37.0 33.0 37.0 5 34.823539359178206 37.0 37.0 37.0 33.0 37.0 6 34.9220944031908 37.0 37.0 37.0 33.0 37.0 7 34.886651180290045 37.0 37.0 37.0 33.0 37.0 8 34.89217714506996 37.0 37.0 37.0 33.0 37.0 9 34.90095614950574 37.0 37.0 37.0 33.0 37.0 10-11 34.81032369795862 37.0 37.0 37.0 33.0 37.0 12-13 34.72556630507328 37.0 37.0 37.0 33.0 37.0 14-15 36.37152019316111 40.0 37.0 40.0 33.0 40.0 16-17 36.32311984614353 40.0 37.0 40.0 33.0 40.0 18-19 36.25774260461309 40.0 37.0 40.0 33.0 40.0 20-21 36.16962085227438 40.0 37.0 40.0 33.0 40.0 22-23 36.0757866432253 40.0 37.0 40.0 33.0 40.0 24-25 35.945115462394924 40.0 37.0 40.0 33.0 40.0 26-27 35.7564427896291 40.0 37.0 40.0 27.0 40.0 28-29 35.64853025346892 40.0 37.0 40.0 27.0 40.0 30-31 35.51887058185483 37.0 37.0 40.0 27.0 40.0 32-33 35.36442874682426 37.0 37.0 40.0 27.0 40.0 34-35 35.252318953984144 37.0 37.0 40.0 27.0 40.0 36-37 35.040802082905984 37.0 37.0 40.0 27.0 40.0 38-39 34.84834891476956 37.0 37.0 40.0 27.0 40.0 40-41 34.61426076650059 37.0 37.0 40.0 24.5 40.0 42-43 34.429734568574496 37.0 33.0 40.0 22.0 40.0 44-45 34.14318039911024 37.0 33.0 40.0 22.0 40.0 46-47 34.00232158063106 37.0 33.0 40.0 22.0 40.0 48-49 33.980765439930835 37.0 33.0 40.0 22.0 40.0 50-51 33.1595037588568 37.0 33.0 38.5 22.0 40.0 52-53 33.28226410459358 37.0 33.0 38.5 22.0 40.0 54-55 33.78101156925392 37.0 33.0 40.0 22.0 40.0 56-57 33.714396551637876 37.0 33.0 40.0 22.0 40.0 58-59 33.541708526245955 37.0 33.0 40.0 22.0 40.0 60-61 33.54620959841821 37.0 33.0 40.0 22.0 40.0 62-63 33.379482310408626 37.0 33.0 40.0 15.0 40.0 64-65 33.15912814834901 37.0 33.0 40.0 15.0 40.0 66-67 32.92278020819053 37.0 33.0 40.0 15.0 40.0 68-69 32.690075802528185 37.0 33.0 40.0 15.0 40.0 70-71 32.46434714566033 37.0 33.0 38.5 10.5 40.0 72-73 31.867515432175978 37.0 33.0 37.0 6.0 40.0 74-75 31.674679023747892 37.0 33.0 37.0 4.0 40.0 76-77 31.33578115978719 37.0 33.0 37.0 2.0 38.5 78-79 31.08737778587174 37.0 33.0 37.0 2.0 37.0 80-81 30.835052327809535 37.0 33.0 37.0 2.0 37.0 82-83 30.57498426513424 37.0 33.0 37.0 2.0 37.0 84-85 30.203674704039432 35.0 33.0 37.0 2.0 37.0 86-87 30.05459398042573 33.0 33.0 37.0 2.0 37.0 88-89 29.87223964425695 33.0 33.0 37.0 2.0 37.0 90-91 29.590853340044205 33.0 30.0 37.0 2.0 37.0 92-93 29.30360761197271 33.0 27.0 37.0 2.0 37.0 94-95 29.072826662317272 33.0 27.0 37.0 2.0 37.0 96-97 28.77282681241138 33.0 27.0 37.0 2.0 37.0 98-99 28.452468072481444 33.0 27.0 37.0 2.0 37.0 100-101 27.7448265062194 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1732662978058137 1101 2 0.20881052151761992 1101 3 0.18828139590132054 1101 4 0.1107146528659797 1101 5 0.07382935680976033 1101 6 0.16256629790812838 1101 7 0.23673221518140508 1101 8 0.1515092745443809 1101 9 0.22265780014615189 1101 10-11 0.22668076166538498 1101 12-13 0.19221554325552148 1101 14-15 0.32970985725440727 1101 16-17 0.27046906284383 1101 18-19 0.32176612398168913 1101 20-21 0.2926475428292079 1101 22-23 0.28820074555585506 1101 24-25 0.20142566586124389 1101 26-27 0.37776531055421714 1101 28-29 0.28400644789989826 1101 30-31 0.29524125460475403 1101 32-33 0.2937276914332756 1101 34-35 0.25855359951819423 1101 36-37 0.19998311985282413 1101 38-39 0.30766483907613207 1101 40-41 0.2710685129511532 1101 42-43 0.16444433800560887 1101 44-45 0.08494360483964414 1101 46-47 0.28534961109563284 1101 48-49 0.33705533289017353 1101 50-51 0.25140321962123124 1101 52-53 0.4378131711999629 1101 54-55 0.5145830966160645 1101 56-57 0.3857193412751272 1101 58-59 0.39585635557966015 1101 60-61 0.26196601233870354 1101 62-63 0.40946697961356904 1101 64-65 0.3901579321595179 1101 66-67 0.4315883279861694 1101 68-69 0.4084178533535763 1101 70-71 0.21411798603133292 1101 72-73 0.27348775683654836 1101 74-75 0.25701879591021637 1101 76-77 0.12482534235160259 1101 78-79 0.21172323365140144 1101 80-81 0.2773130968470561 1101 82-83 0.2161552202203545 1101 84-85 0.25591214271260654 1101 86-87 0.3442145634175269 1101 88-89 0.19485305870221126 1101 90-91 0.1325613817074185 1101 92-93 0.14143451403452545 1101 94-95 0.15819001597610338 1101 96-97 0.07997616406552055 1101 98-99 0.26779623083717397 1101 100-101 0.2598340969808355 1102 1 0.14966875025847415 1102 2 0.13306522695623713 1102 3 0.1452904945747271 1102 4 0.2105432712856654 1102 5 0.09029324523381632 1102 6 0.20525289021752968 1102 7 0.2211227793354169 1102 8 0.14914506243314207 1102 9 0.11492666637975191 1102 10-11 0.1634444337565597 1102 12-13 0.1580398199077777 1102 14-15 0.3165052407929423 1102 16-17 0.35284282427478075 1102 18-19 0.234750479038091 1102 20-21 0.28083441485662064 1102 22-23 0.3705652145317089 1102 24-25 0.4148618759900984 1102 26-27 0.33261625541936013 1102 28-29 0.39547681271186974 1102 30-31 0.41823860776601407 1102 32-33 0.36050844879858346 1102 34-35 0.2642686482714751 1102 36-37 0.2993116833110392 1102 38-39 0.35395354205716245 1102 40-41 0.28511553201843753 1102 42-43 0.32368339925611167 1102 44-45 0.3502600450436333 1102 46-47 0.284971302405566 1102 48-49 0.2983780949855728 1102 50-51 0.2211560894222231 1102 52-53 0.33681717866565464 1102 54-55 0.4038400363990675 1102 56-57 0.44619452569825313 1102 58-59 0.4918137976463015 1102 60-61 0.4881833900607546 1102 62-63 0.5202175568978973 1102 64-65 0.5574541563630433 1102 66-67 0.5913105213530869 1102 68-69 0.48153043673541873 1102 70-71 0.4860085737942299 1102 72-73 0.3921844409032751 1102 74-75 0.4297420783075445 1102 76-77 0.39850204575116877 1102 78-79 0.4434856384179042 1102 80-81 0.6196926603963071 1102 82-83 0.432021369581836 1102 84-85 0.5189059602080341 1102 86-87 0.49534191575909503 1102 88-89 0.5177161623293252 1102 90-91 0.42980813303111987 1102 92-93 0.3600668308513377 1102 94-95 0.32749061518835987 1102 96-97 0.4279559805508626 1102 98-99 0.5136853582492016 1102 100-101 0.38276209669677286 1103 1 0.09243337655019346 1103 2 0.022433799610809046 1103 3 0.004537029171949314 1103 4 0.040241354198080614 1103 5 0.034543107946809926 1103 6 -0.005299970014824851 1103 7 0.03281145062940993 1103 8 -0.004610599204490029 1103 9 -0.005146534302951977 1103 10-11 0.07126202228568701 1103 12-13 0.1699543314572125 1103 14-15 0.3069127643711127 1103 16-17 0.2195975380335895 1103 18-19 0.21076114187084016 1103 20-21 0.2921642999071068 1103 22-23 0.21917460521319754 1103 24-25 0.17934814746787708 1103 26-27 0.2421997639715272 1103 28-29 0.18933550123774978 1103 30-31 0.2705440469754734 1103 32-33 0.12017143554221121 1103 34-35 0.11213177642876104 1103 36-37 0.10582950239658828 1103 38-39 0.10220739779450838 1103 40-41 0.1850683345848907 1103 42-43 0.16434114783491793 1103 44-45 0.1889715158064149 1103 46-47 0.04817095255146597 1103 48-49 0.046715519835004216 1103 50-51 0.13648367926973748 1103 52-53 0.3672194715820041 1103 54-55 0.4641071217092332 1103 56-57 0.3780798041363127 1103 58-59 0.47280280718261025 1103 60-61 0.5017056870072878 1103 62-63 0.4902611207364487 1103 64-65 0.43181315912163853 1103 66-67 0.528348037417544 1103 68-69 0.5356604979991886 1103 70-71 0.564883988647523 1103 72-73 0.3146056871084504 1103 74-75 0.4023914692605821 1103 76-77 0.4108979997056217 1103 78-79 0.5028690848738258 1103 80-81 0.4423644710234811 1103 82-83 0.5116163577217385 1103 84-85 0.5188718037004882 1103 86-87 0.5232789020046695 1103 88-89 0.5604326622448319 1103 90-91 0.4585631962153016 1103 92-93 0.33536009983990667 1103 94-95 0.35904465410605724 1103 96-97 0.2578399949123167 1103 98-99 0.22581031104283156 1103 100-101 0.41678262132786514 1104 1 -0.08138514224455307 1104 2 -0.05808574809582723 1104 3 -0.1103093366405048 1104 4 -0.14160102925418983 1104 5 -0.17235491540531456 1104 6 -0.10469354879609938 1104 7 0.022399240226363304 1104 8 -0.03217006032716796 1104 9 -0.06648885061752452 1104 10-11 -0.03590481260832945 1104 12-13 -0.01721505309142657 1104 14-15 -0.0038556566605691955 1104 16-17 -0.009241340604148718 1104 18-19 -0.016753816937757904 1104 20-21 -0.060345624431413114 1104 22-23 -0.08070134956075492 1104 24-25 -0.12721197117202365 1104 26-27 -0.043600189824559266 1104 28-29 -0.06703728022480959 1104 30-31 -0.2216368978142782 1104 32-33 -0.1755614333825548 1104 34-35 -0.14739277199041823 1104 36-37 0.023835532508179824 1104 38-39 -1.504289419145266E-4 1104 40-41 0.019467580446999477 1104 42-43 -0.055659842935071424 1104 44-45 -0.037617855248818444 1104 46-47 -0.10181193196557814 1104 48-49 -0.13155803326858972 1104 50-51 -0.08772907884248582 1104 52-53 -0.021579359089841432 1104 54-55 -0.04911928028004553 1104 56-57 0.033453328196074494 1104 58-59 -0.05050292982161153 1104 60-61 0.0066413888480951755 1104 62-63 0.018093832654358266 1104 64-65 -0.03634171634151784 1104 66-67 0.03910725746199972 1104 68-69 0.041393961797624 1104 70-71 0.01917182365934167 1104 72-73 -0.026696563400879114 1104 74-75 -0.060911343462695555 1104 76-77 -0.10690028432741272 1104 78-79 -0.001156158353687431 1104 80-81 0.057391357323638204 1104 82-83 0.026636780688583883 1104 84-85 0.09129688409591452 1104 86-87 0.01869195943371693 1104 88-89 -0.05761332800816277 1104 90-91 -0.010996836727112935 1104 92-93 0.05369259689658534 1104 94-95 0.012526829817744556 1104 96-97 0.022278975179460758 1104 98-99 -0.058125568025044316 1104 100-101 0.01877378083270642 1105 1 0.09291712431958743 1105 2 0.08561358400416452 1105 3 0.04754752178067534 1105 4 0.13670265288143213 1105 5 0.13046357928192975 1105 6 0.06595022891647773 1105 7 0.035066858155339276 1105 8 -0.0027530875086654305 1105 9 0.06834724425552707 1105 10-11 -0.0013785195649731463 1105 12-13 0.10984953494524774 1105 14-15 0.1193946196767044 1105 16-17 0.05066799941799616 1105 18-19 0.08927539830965259 1105 20-21 0.20775416818219128 1105 22-23 0.1386274120632791 1105 24-25 0.1744437557624252 1105 26-27 0.12732262626987279 1105 28-29 0.041276323203618404 1105 30-31 0.1017164409255713 1105 32-33 0.16446595287921895 1105 34-35 0.12376664122026426 1105 36-37 0.11521008929294396 1105 38-39 0.2039691234450629 1105 40-41 0.21707961228700867 1105 42-43 0.19597649105827486 1105 44-45 0.09566926282427346 1105 46-47 -0.031248669733230372 1105 48-49 0.08442980497972741 1105 50-51 0.11368005528129999 1105 52-53 0.02236219876986212 1105 54-55 0.18254086869784913 1105 56-57 0.18170678277598995 1105 58-59 0.32078254559335306 1105 60-61 0.23967457099520573 1105 62-63 0.28669078975795514 1105 64-65 0.18319258781419734 1105 66-67 0.32959727616268 1105 68-69 0.3366388579281292 1105 70-71 0.27780905791216526 1105 72-73 0.15780027630991 1105 74-75 0.21268071122184296 1105 76-77 0.16029446123687663 1105 78-79 0.23775387748524324 1105 80-81 0.3525092531426246 1105 82-83 0.3883495186645547 1105 84-85 0.39504908662614824 1105 86-87 0.26128403385085264 1105 88-89 0.2812843506939906 1105 90-91 0.2781074034852722 1105 92-93 0.36911818542140296 1105 94-95 0.4613306945607931 1105 96-97 0.4337635428300928 1105 98-99 0.3822658685438647 1105 100-101 0.3802950155371114 1106 1 -0.045765100451234275 1106 2 -0.06503448527127631 1106 3 -0.04786642245570505 1106 4 -0.06768274013994358 1106 5 -0.09686906561164221 1106 6 -0.09047248578869471 1106 7 -0.013394225169662377 1106 8 -0.011493734483416063 1106 9 -0.020895805120197508 1106 10-11 -0.11936614985695826 1106 12-13 -0.08751028269752936 1106 14-15 -0.07354554158316517 1106 16-17 -0.0056248000414029775 1106 18-19 9.511146280587468E-4 1106 20-21 -0.015254472212724579 1106 22-23 -0.0981846042244996 1106 24-25 -0.08388263765393589 1106 26-27 -0.039947614890266436 1106 28-29 -0.03218670329813733 1106 30-31 -0.09990044075955495 1106 32-33 -0.08963970200306903 1106 34-35 -0.11577867128221442 1106 36-37 -0.06871452496663721 1106 38-39 -0.04473212066393728 1106 40-41 -0.059692366628333104 1106 42-43 -0.006733487035376129 1106 44-45 -0.05203104697361738 1106 46-47 -0.14960114137473823 1106 48-49 -0.08991756522796379 1106 50-51 -0.12997495708365392 1106 52-53 -0.08119563889476922 1106 54-55 -0.08298844471831757 1106 56-57 0.038612991582624545 1106 58-59 0.060945550363250334 1106 60-61 -0.0686809304157805 1106 62-63 -0.0237405003051947 1106 64-65 0.040126397662056945 1106 66-67 0.13277329942369676 1106 68-69 0.065652628309671 1106 70-71 0.10709392127907336 1106 72-73 -0.0177502772178606 1106 74-75 -0.013320421372608138 1106 76-77 -0.10614854181985933 1106 78-79 -0.01701737491993427 1106 80-81 0.10173459978156174 1106 82-83 0.08490668533836043 1106 84-85 0.13382458388111473 1106 86-87 0.18991091377522196 1106 88-89 0.10826896866543834 1106 90-91 0.1232354231422903 1106 92-93 0.18652603522097522 1106 94-95 0.18795047118271313 1106 96-97 0.21721116668183527 1106 98-99 0.2745129898663059 1106 100-101 0.19815510998343555 1107 1 0.06259629904915087 1107 2 0.07071721986241641 1107 3 0.05798291674422629 1107 4 0.18047273012277998 1107 5 0.011710701082456865 1107 6 -0.02528790418771365 1107 7 0.00943226594174007 1107 8 0.018141924340881133 1107 9 0.08537555111656303 1107 10-11 0.06514784703146148 1107 12-13 -0.004686956414765575 1107 14-15 0.06933109620818101 1107 16-17 0.09387391263911837 1107 18-19 0.06358887665746948 1107 20-21 0.169048097370883 1107 22-23 0.05671126304174123 1107 24-25 0.15641035727605868 1107 26-27 0.10670061320952584 1107 28-29 -0.00798769959098422 1107 30-31 0.12520768839297602 1107 32-33 0.1260289817183775 1107 34-35 0.123466968777457 1107 36-37 0.19919399293931406 1107 38-39 0.1388881262226107 1107 40-41 0.1367512693589319 1107 42-43 0.09487282802420793 1107 44-45 0.21295511961422875 1107 46-47 0.023492520502500724 1107 48-49 0.060770096995682366 1107 50-51 0.0442819139298436 1107 52-53 0.1549589985812858 1107 54-55 0.2641985520836485 1107 56-57 0.26699926101244387 1107 58-59 0.20097976521204686 1107 60-61 0.20337961532869997 1107 62-63 0.22678353608973367 1107 64-65 0.16109610953402864 1107 66-67 0.18881786786879928 1107 68-69 0.15752553979946526 1107 70-71 0.17264990658924262 1107 72-73 0.25801910070186196 1107 74-75 0.2546067026147867 1107 76-77 0.22253883503076466 1107 78-79 0.21700130235518955 1107 80-81 0.18391380266951174 1107 82-83 0.28976957317569685 1107 84-85 0.3919875833250259 1107 86-87 0.4216484615355647 1107 88-89 0.36367309305337514 1107 90-91 0.3444030415010495 1107 92-93 0.3185158718733412 1107 94-95 0.4220923913123684 1107 96-97 0.46213450334452233 1107 98-99 0.49275251355061656 1107 100-101 0.6236848972130211 1108 1 -0.06386791402141156 1108 2 -0.03363209707856285 1108 3 -0.04897213827169011 1108 4 0.008135074957337451 1108 5 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12546.0 10 13074.0 11 13163.0 12 13841.0 13 14766.0 14 15079.0 15 17285.0 16 20811.0 17 25694.0 18 22456.0 19 17710.0 20 18063.0 21 18617.0 22 20709.0 23 23510.0 24 27092.0 25 32082.0 26 36510.0 27 42829.0 28 51802.0 29 62919.0 30 77506.0 31 97264.0 32 126076.0 33 166795.0 34 231722.0 35 340053.0 36 528888.0 37 904565.0 38 826793.0 39 304.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.26092964660701 23.53037266515171 12.979139837237296 23.229557851003985 2 14.364466734845408 21.486785198942496 42.79700280386737 21.35174526234472 3 17.80202522133269 30.58630055017037 29.356603607914867 22.25507062058207 4 11.405700511273892 18.116451772197685 42.890621208535606 27.587226507992813 5 12.274497737284893 38.7189455172224 36.046289972757364 12.960266772735347 6 25.926330809417504 41.189425769957765 20.30683238390471 12.577411036720024 7 24.304163710646577 32.34783209072088 27.08195538602704 16.266048812605504 8 23.533230335420306 36.624713695487074 21.643220299127552 18.198835669965067 9 25.91347274741263 15.601106894022552 23.85243047390714 34.632989884657675 10-11 23.443504938472657 27.164051151084518 29.764657381342445 19.627786529100376 12-13 24.073940425344563 25.066876364997675 29.431016499484613 21.428166710173148 14-15 21.727863754009526 26.29756954468426 27.1739108681284 24.800655833177814 16-17 20.137492721654166 28.51217789997129 28.884566529553368 22.465762848821175 18-19 19.750776860070623 29.559458455075706 30.224512966930643 20.465251717923024 20-21 20.84489474900763 27.96110661384688 29.347675992847517 21.846322644297974 22-23 21.25471420580704 28.64302167458997 28.63654261221786 21.46572150738513 24-25 21.132735791709298 28.550903191752823 29.344163462572766 20.972197553965113 26-27 21.135064185244147 28.223521147759644 28.914516902097613 21.72689776489859 28-29 20.500116072777633 29.47573128351476 28.528499869418123 21.49565277428948 30-31 21.796103656992933 28.490563583366775 28.26944994511559 21.443882814524706 32-33 21.74822363621991 28.82383504457295 28.11066288562929 21.31727843357785 34-35 22.07005042161435 27.810724824464938 29.13450483453125 20.98471991938946 36-37 22.07933124068791 28.18274558147959 28.90129611266264 20.83662706516986 38-39 22.60089576164255 27.753388874824513 29.53679457019551 20.108920793337422 40-41 21.486774167725667 27.64306513103255 29.808779531104594 21.061381170137185 42-43 21.93582448788809 27.800328731237762 28.675978091755315 21.587868689118835 44-45 21.496674333600293 28.796192191084884 28.63072501241098 21.076408462903846 46-47 22.400005342103118 28.02051518788895 27.91682295048135 21.662656519526582 48-49 22.554295174582332 27.064206168078186 28.76130089891678 21.6201977584227 50-51 22.206411494838342 27.636624024167933 27.81194853966144 22.34501594133228 52-53 21.093114548275143 28.265965763316103 28.26496174762986 22.375957940778886 54-55 21.61625673538607 28.627621401341795 27.59799737323394 22.15812449003819 56-57 22.381027053134037 27.489521850125765 28.113506495135702 22.015944601604495 58-59 21.881116860760727 27.24232048771927 29.260120078175383 21.61644257334462 60-61 21.736699469824973 29.23953145129895 27.66405676203931 21.35971231683677 62-63 22.17081852319036 28.23599377052215 26.967034885427122 22.626152820860373 64-65 20.839242643496693 28.480226797118984 27.566532175386527 23.113998383997792 66-67 21.922295050392883 26.74222087801081 28.227035301599912 23.108448769996397 68-69 20.687564148387104 27.792437977825955 27.810039411415083 23.70995846237186 70-71 21.217285110027102 27.512599468248837 28.36968303693733 22.900432384786725 72-73 22.165054198035634 27.271971894615653 28.723729533967123 21.83924437338159 74-75 21.921435333766983 28.510338491817645 27.52083296919936 22.047393205216018 76-77 21.333863289446658 28.19973589457532 28.444735713336367 22.02166510264166 78-79 21.170471956522256 28.048623898926472 28.053117372156606 22.727786772394662 80-81 21.207512704238553 28.326678806864834 28.881870184354952 21.583938304541658 82-83 22.31396333786694 27.010893827174122 29.33498722390854 21.340155611050392 84-85 22.97240466831783 27.425947064453577 27.81740676853658 21.78424149869202 86-87 21.585493767754578 28.56490644892104 28.138100614980083 21.711499168344297 88-89 22.35595138451314 27.18038326859837 28.257698444793462 22.20596690209503 90-91 22.19120329310919 26.65503216459928 28.9732334551963 22.180531087095233 92-93 22.098146040098243 27.78823863592917 28.311118582257254 21.802496741715334 94-95 20.89960095626133 27.360467839716325 28.95577811398668 22.784153090035662 96-97 21.44128997621153 27.414449626525645 28.209661719200845 22.934598678061985 98-99 20.751513298991803 28.401114429456214 28.239198953719107 22.608173317832875 100-101 21.447925200490044 26.905375713709486 28.068380822103133 23.578318263697334 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 139.0 1 87.5 2 67.5 3 94.5 4 76.0 5 71.5 6 106.0 7 186.0 8 308.5 9 360.0 10 308.0 11 302.0 12 411.5 13 609.0 14 784.0 15 987.0 16 1318.0 17 1614.5 18 1825.5 19 2084.0 20 2375.5 21 2675.5 22 2996.0 23 3394.0 24 3737.5 25 4092.5 26 4947.0 27 6383.5 28 9802.5 29 19939.5 30 36033.5 31 54249.5 32 78922.0 33 113415.5 34 159258.0 35 192580.5 36 217164.5 37 254496.5 38 252901.0 39 203133.0 40 156699.0 41 123210.0 42 105061.5 43 99708.0 44 97765.5 45 104598.5 46 113913.5 47 125456.0 48 139915.5 49 137760.5 50 124114.5 51 119468.0 52 116927.5 53 113282.5 54 108738.0 55 97657.0 56 87623.5 57 73387.0 58 58840.0 59 54675.5 60 47161.5 61 36040.0 62 27693.5 63 22520.0 64 17803.0 65 11899.5 66 8351.0 67 6517.5 68 5309.5 69 4274.5 70 3396.0 71 2698.5 72 2111.5 73 1702.0 74 1367.0 75 1101.5 76 898.0 77 699.0 78 551.5 79 442.0 80 376.5 81 318.5 82 265.0 83 207.0 84 163.0 85 138.0 86 110.0 87 92.0 88 75.5 89 57.0 90 43.5 91 36.0 92 27.5 93 22.5 94 16.5 95 13.5 96 12.0 97 7.0 98 3.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.0025095735025864E-4 2 0.0039024468341650217 3 7.504705450317349E-5 4 6.504078056941702E-4 5 0.0020512861564200755 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.5015684834391165E-5 12-13 8.755489692036907E-5 14-15 0.0033521017678084164 16-17 0.004740472276117126 18-19 0.00376486056757587 20-21 0.0 22-23 0.0 24-25 5.003136966878233E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 3.0018821801269395E-4 42-43 3.7523527251586744E-5 44-45 5.628529087738012E-4 46-47 0.7258926346819455 48-49 0.5221398817058296 50-51 0.40070123967727767 52-53 0.337474096258354 54-55 0.328330863451384 56-57 0.2956103476880004 58-59 0.2961481849119398 60-61 0.27227071373751344 62-63 0.26619190232275636 64-65 0.2624770731248493 66-67 0.250757224779937 68-69 0.22965649462212806 70-71 0.24326502717203685 72-73 0.8002142343249218 74-75 0.529394430307803 76-77 0.6212895485469389 78-79 0.3486310916944924 80-81 0.30292743550205975 82-83 0.283565295440241 84-85 0.27523507239038875 86-87 0.24926879153229073 88-89 0.2521455952882457 90-91 0.2626646907611072 92-93 0.2204757382879065 94-95 0.18958136751743343 96-97 0.14640429549327427 98-99 0.15539743419123792 100-101 0.15064445407270358 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3997492.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.917653956869128 #Duplication Level Percentage of deduplicated Percentage of total 1 80.80651343603364 22.55928279568283 2 9.972458697542638 5.5681530203433045 3 3.359590937103569 2.8137569165607332 4 1.515594571993754 1.692473791993232 5 0.8531592388714608 1.1909102200459645 6 0.5346332761346685 0.895542407817296 7 0.3725081954697934 0.7279688428056419 8 0.2662784315004073 0.5947095285445001 9 0.21959694684745845 0.5517568414865095 >10 1.4905731589278997 8.343411296873244 >50 0.24526409621584816 4.8342428710764 >100 0.281074189993378 16.91533522746139 >500 0.048859601594940176 9.420797515853241 >1k 0.030920375179085594 16.11758116832562 >5k 0.0018930841946378934 3.5495633631912002 >10k+ 0.0010817623969359392 4.224514191939045 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 19465 0.486930305301424 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 19408 0.4855044112658637 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 16138 0.4037031218574046 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 14869 0.3719582178025622 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 14161 0.35424711293981326 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 12924 0.3233027107996714 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 12737 0.31862477773564024 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 12535 0.31357160939909323 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 12384 0.30979424098910013 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 11385 0.2848035718395434 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 11341 0.2837028817068302 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 10513 0.2629898946639543 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 9836 0.2460542760310715 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 9726 0.24330255069928844 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 9234 0.23099483376076801 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 8111 0.20290221969174674 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 7431 0.18589155400436072 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 7428 0.18581650694985757 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7020 0.17561010753742598 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 6716 0.16800533934777107 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 6528 0.16330239059890553 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 6422 0.16065072800646005 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 6321 0.15812414383818654 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 6143 0.15367135193766493 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 6118 0.15304595981680513 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 6067 0.15177015989025117 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 6061 0.15162006578124484 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 5973 0.14941868551581844 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 5424 0.13568507454173767 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 5188 0.12978137292082137 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 5118 0.12803027498241398 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 5103 0.1276550397098981 No Hit GTATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATT 5073 0.1269045691648664 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 4969 0.12430293794208969 No Hit GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG 4691 0.11734857755812894 No Hit GATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAG 4651 0.1163479501647533 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 4523 0.11314594250595124 No Hit ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT 4478 0.11202023668840362 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 4472 0.11187014257939727 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 4443 0.11114468771919994 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 4429 0.11079446813151847 No Hit ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA 4369 0.10929352704145498 No Hit GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA 4312 0.10786763300589469 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 4297 0.10749239773337883 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 4276 0.10696706835185661 No Hit ATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGT 4189 0.10479070377126458 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 4006 0.100212833446571 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.5015684834391165E-5 0.0 12-13 0.0 0.0 0.0 3.752352725158675E-5 0.0 14-15 0.0 0.0 0.0 5.003136966878233E-5 0.0 16-17 0.0 0.0 0.0 5.003136966878233E-5 0.0 18-19 0.0 0.0 0.0 5.003136966878233E-5 0.0 20-21 0.0 0.0 0.0 5.003136966878233E-5 0.0 22-23 0.0 0.0 0.0 2.0012547867512932E-4 0.0 24-25 0.0 0.0 0.0 3.252039028470851E-4 0.0 26-27 0.0 0.0 0.0 7.004391753629526E-4 0.0 28-29 0.0 0.0 0.0 0.0016510351990698168 0.0 30-31 0.0 0.0 0.0 0.006816774117371592 0.0 32-33 0.0 0.0 0.0 0.022951890835553893 0.0 34-35 2.5015684834391165E-5 0.0 0.0 0.04831779525762653 0.0 36-37 2.5015684834391165E-5 0.0 0.0 0.08818028904122885 0.0 38-39 2.5015684834391165E-5 0.0 0.0 0.14505344851221716 0.0 40-41 2.5015684834391165E-5 0.0 0.0 0.20738002727710275 0.0 42-43 2.5015684834391165E-5 0.0 0.0 0.2796503407636588 0.0 44-45 2.5015684834391165E-5 0.0 0.0 0.3599381812396373 0.0 46-47 2.5015684834391165E-5 0.0 0.0 0.46320292823600395 0.0 48-49 2.5015684834391165E-5 0.0 0.0 0.574797898282223 0.0 50-51 2.5015684834391165E-5 0.0 0.0 0.6879438407881742 0.0 52-53 2.5015684834391165E-5 0.0 0.0 0.8064681555335196 0.0 54-55 2.5015684834391165E-5 0.0 0.0 0.9355365814365608 0.0 56-57 2.5015684834391165E-5 0.0 0.0 1.0923348939785245 0.0 58-59 2.5015684834391165E-5 0.0 0.0 1.2778512127103694 0.0 60-61 2.5015684834391165E-5 0.0 0.0 1.4723856858250124 0.0 62-63 2.5015684834391165E-5 0.0 0.0 1.6701847058105432 0.0 64-65 2.5015684834391165E-5 0.0 0.0 1.8820800642002538 0.0 66-67 2.5015684834391165E-5 0.0 0.0 2.1212300112170333 0.0 68-69 2.5015684834391165E-5 0.0 0.0 2.3860835744011495 0.0 70-71 2.5015684834391165E-5 0.0 0.0 2.66300720551786 0.0 72-73 2.5015684834391165E-5 0.0 0.0 2.9755781875235776 0.0 74-75 2.5015684834391165E-5 0.0 0.0 3.273715119379851 0.0 76-77 2.5015684834391165E-5 0.0 0.0 3.6143286840849216 0.0 78-79 2.5015684834391165E-5 0.0 0.0 3.9983694776624943 0.0 80-81 2.5015684834391165E-5 0.0 0.0 4.43819524842076 0.0 82-83 2.5015684834391165E-5 0.0 0.0 4.90895291347675 0.0 84-85 2.5015684834391165E-5 0.0 0.0 5.359973203198406 0.0 86-87 2.5015684834391165E-5 0.0 0.0 5.864414487883904 0.0 88-89 2.5015684834391165E-5 0.0 0.0 6.4039652862344685 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3235 0.0 44.21514 2 ACCTGGG 3265 0.0 42.93796 3 GTACCTG 3560 0.0 40.71081 1 CCTGGGG 3290 0.0 36.709393 4 CTGGGGG 3175 0.0 34.160534 5 GTACCCG 325 0.0 30.603724 1 GTACTAG 2835 0.0 29.57049 1 CTGTGCG 3100 0.0 29.183123 9 TAGTACT 3285 0.0 28.114616 4 TGGGGGG 10240 0.0 27.519062 6 ACTGTGC 3495 0.0 26.695686 8 GCATCGT 2785 0.0 26.188917 8 GTACATG 13610 0.0 25.786858 1 CATGGGG 10075 0.0 25.76134 4 GTACTGT 3720 0.0 25.589968 6 TATTCTC 3090 0.0 25.443832 5 TAGGCAT 3630 0.0 25.051142 5 TACCCCG 265 1.8189894E-12 25.0216 5 ATGGGGG 9140 0.0 24.821169 5 CGTTAAC 745 0.0 24.794004 1 >>END_MODULE