##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931070_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 300488 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.019268656319053 33.0 33.0 33.0 27.0 33.0 2 29.929361571843135 33.0 33.0 33.0 22.0 33.0 3 29.960930220175182 33.0 33.0 33.0 22.0 33.0 4 33.42404022789596 37.0 37.0 37.0 27.0 37.0 5 33.374890178642744 37.0 37.0 37.0 27.0 37.0 6 33.47213865445542 37.0 37.0 37.0 27.0 37.0 7 33.44170149889513 37.0 37.0 37.0 27.0 37.0 8 33.426908894864354 37.0 37.0 37.0 27.0 37.0 9 33.45412462394505 37.0 37.0 37.0 27.0 37.0 10-11 33.341241580362606 37.0 37.0 37.0 27.0 37.0 12-13 33.2301805729347 37.0 37.0 37.0 22.0 37.0 14-15 34.74565872846836 40.0 37.0 40.0 22.0 40.0 16-17 34.66545086659035 40.0 37.0 40.0 22.0 40.0 18-19 34.5911417427651 40.0 37.0 40.0 18.5 40.0 20-21 34.49887516306808 40.0 37.0 40.0 15.0 40.0 22-23 34.39592096855782 38.5 37.0 40.0 15.0 40.0 24-25 34.26427344852373 37.0 37.0 40.0 15.0 40.0 26-27 34.072490415590636 37.0 37.0 40.0 15.0 40.0 28-29 33.95115112749927 37.0 37.0 40.0 15.0 40.0 30-31 33.82437734618354 37.0 35.0 40.0 15.0 40.0 32-33 33.66523787971566 37.0 33.0 40.0 10.5 40.0 34-35 33.56532374004952 37.0 33.0 40.0 6.0 40.0 36-37 33.36917614014537 37.0 33.0 40.0 6.0 40.0 38-39 33.18085247996592 37.0 33.0 40.0 6.0 40.0 40-41 32.95998509091878 37.0 33.0 40.0 6.0 40.0 42-43 32.754283032933095 37.0 33.0 40.0 2.0 40.0 44-45 32.500244602113895 37.0 33.0 40.0 2.0 40.0 46-47 32.40491467213333 37.0 33.0 40.0 2.0 40.0 48-49 32.39521544953543 37.0 33.0 40.0 2.0 40.0 50-51 31.579637123612258 37.0 33.0 38.5 2.0 40.0 52-53 31.69746545619126 37.0 33.0 38.5 2.0 40.0 54-55 32.155691741433934 37.0 33.0 40.0 2.0 40.0 56-57 32.05082399297143 37.0 33.0 40.0 2.0 40.0 58-59 31.88873765341711 37.0 33.0 40.0 2.0 40.0 60-61 31.889702750193017 37.0 33.0 40.0 2.0 40.0 62-63 31.664935039003222 37.0 33.0 40.0 2.0 40.0 64-65 31.4127602433375 37.0 33.0 40.0 2.0 40.0 66-67 31.091784031309068 37.0 33.0 37.0 2.0 40.0 68-69 30.793745507308113 37.0 33.0 37.0 2.0 40.0 70-71 30.497865139373285 37.0 33.0 37.0 2.0 40.0 72-73 29.89671634141796 37.0 27.0 37.0 2.0 38.5 74-75 29.685736202444026 37.0 27.0 37.0 2.0 37.0 76-77 29.302765834242965 37.0 27.0 37.0 2.0 37.0 78-79 29.01356293762147 35.0 27.0 37.0 2.0 37.0 80-81 28.709818694922923 33.0 27.0 37.0 2.0 37.0 82-83 28.388924016932457 33.0 27.0 37.0 2.0 37.0 84-85 27.944182463193208 33.0 27.0 37.0 2.0 37.0 86-87 27.71732814621549 33.0 27.0 37.0 2.0 37.0 88-89 27.40299612630122 33.0 27.0 37.0 2.0 37.0 90-91 26.989373951705225 33.0 22.0 37.0 2.0 37.0 92-93 26.567704866750084 33.0 22.0 37.0 2.0 37.0 94-95 26.14311553206784 33.0 15.0 37.0 2.0 37.0 96-97 25.691937781209234 33.0 15.0 37.0 2.0 37.0 98-99 25.21322149303799 33.0 2.0 37.0 2.0 37.0 100-101 24.35569640052182 33.0 2.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1658346909580395 1101 2 0.4955185091364456 1101 3 0.3557394203308597 1101 4 0.39395622294815524 1101 5 0.5896626777246752 1101 6 0.8400931321571221 1101 7 0.662613607030039 1101 8 0.6398494717590424 1101 9 0.5584223528923573 1101 10-11 0.8045397849886342 1101 12-13 0.5463638783751676 1101 14-15 0.7061577229114917 1101 16-17 0.6627104184910309 1101 18-19 0.9581123616980776 1101 20-21 0.9161736695841398 1101 22-23 0.6507842849536374 1101 24-25 0.7650526048126594 1101 26-27 1.2863891833280192 1101 28-29 1.1228751793412428 1101 30-31 1.229608177536221 1101 32-33 1.307366016965517 1101 34-35 1.1638425726622685 1101 36-37 0.6018778073917659 1101 38-39 0.8612016736325572 1101 40-41 0.5874970424496908 1101 42-43 0.9271494820439088 1101 44-45 0.8162700781946981 1101 46-47 1.128030487927333 1101 48-49 0.9630381211599826 1101 50-51 1.2115099761930033 1101 52-53 0.8941049843415598 1101 54-55 1.0413644178097812 1101 56-57 0.9340145925064292 1101 58-59 1.1277906200750394 1101 60-61 1.1487135703875566 1101 62-63 1.1873029188422564 1101 64-65 1.7056372598792002 1101 66-67 1.456329061257719 1101 68-69 1.6033183089028746 1101 70-71 1.32908657890032 1101 72-73 1.427912124198226 1101 74-75 1.8160438895263695 1101 76-77 1.3310097679138622 1101 78-79 1.12148010927638 1101 80-81 1.2987448414751235 1101 82-83 1.119408113974675 1101 84-85 1.103442287641041 1101 86-87 1.4312744714729924 1101 88-89 0.8668988200055701 1101 90-91 0.8923397007238663 1101 92-93 1.1145219720704063 1101 94-95 1.2653838194297933 1101 96-97 0.8536399008484992 1101 98-99 0.9518561130077536 1101 100-101 1.1530302717038197 1102 1 -0.11790627064136316 1102 2 -0.11585187083647597 1102 3 -0.2798581943956151 1102 4 -0.19468492013944427 1102 5 0.06190650568537848 1102 6 -0.23513804924465376 1102 7 0.01687569904167674 1102 8 -0.09293075466909784 1102 9 -0.14255422648712113 1102 10-11 -0.11356726424364183 1102 12-13 -0.2053069654009363 1102 14-15 -0.09780620990906641 1102 16-17 0.043814039612804834 1102 18-19 0.0456011987332019 1102 20-21 0.08686620552168733 1102 22-23 -0.25048469208666546 1102 24-25 -0.16849534552294898 1102 26-27 -0.4049080252105739 1102 28-29 -0.1042659493425262 1102 30-31 0.23392212332022666 1102 32-33 0.17019118332970606 1102 34-35 -0.30511718304884283 1102 36-37 0.1628636052196626 1102 38-39 -0.09645828085135122 1102 40-41 -0.1576848778683484 1102 42-43 0.0233238259496531 1102 44-45 0.4324441340239247 1102 46-47 0.33972351359494724 1102 48-49 0.49422960561825136 1102 50-51 -0.10103143480576193 1102 52-53 -0.06771047384413365 1102 54-55 0.20441572423676746 1102 56-57 0.30237082792255876 1102 58-59 0.6664432862231777 1102 60-61 0.23385458384073843 1102 62-63 0.06739827216809857 1102 64-65 -0.21388136426737603 1102 66-67 -0.5161524298263132 1102 68-69 -0.04134651407410317 1102 70-71 0.08652615485169335 1102 72-73 -0.12335109131181454 1102 74-75 -0.20451757159770523 1102 76-77 -0.31371267036574224 1102 78-79 -0.5572209537259489 1102 80-81 -0.059226235642960035 1102 82-83 -0.10177703280031025 1102 84-85 0.3536295373745695 1102 86-87 0.1950301249745614 1102 88-89 0.4425117027872396 1102 90-91 0.5518260603009679 1102 92-93 0.2706666440952965 1102 94-95 -0.0542730814430783 1102 96-97 0.17609114389865255 1102 98-99 0.3420197404672045 1102 100-101 0.5058807888011607 1103 1 0.11901697271883549 1103 2 0.12169343126770471 1103 3 0.11820530968833864 1103 4 0.16170064809769258 1103 5 0.3475666993349691 1103 6 0.23275831035939376 1103 7 0.11007632650962762 1103 8 0.12140905168131155 1103 9 0.059993509759969754 1103 10-11 0.050453398925071724 1103 12-13 0.4452447736589491 1103 14-15 0.3834779357067717 1103 16-17 0.4280705441835835 1103 18-19 0.3826623768583701 1103 20-21 0.2801101066413949 1103 22-23 0.14895815964698755 1103 24-25 -0.08135302930074317 1103 26-27 -0.05573022459392263 1103 28-29 0.271700946313878 1103 30-31 0.2029949853313724 1103 32-33 -0.09968765044538941 1103 34-35 -0.2981257836871336 1103 36-37 -0.1389295467249454 1103 38-39 -0.4535740919618618 1103 40-41 0.06072535635028942 1103 42-43 -0.1207639113792851 1103 44-45 0.0370176180938131 1103 46-47 -0.39659119403744114 1103 48-49 -0.09987706393332729 1103 50-51 -0.19411035512814934 1103 52-53 -0.04426156379923896 1103 54-55 -0.08466579250472961 1103 56-57 -0.06152001429088472 1103 58-59 0.15317720735693996 1103 60-61 -0.1980435067093822 1103 62-63 -0.04342284537395358 1103 64-65 -0.11527153033001625 1103 66-67 -0.13472223563580243 1103 68-69 -0.12306610081072833 1103 70-71 -0.0031855344434852384 1103 72-73 -0.5172765524466634 1103 74-75 -0.15192656169885765 1103 76-77 0.15515734640279533 1103 78-79 0.4289720467608795 1103 80-81 0.5838202294406116 1103 82-83 0.6386892407973583 1103 84-85 0.5241953403712678 1103 86-87 0.2871759571809811 1103 88-89 0.5866264861485178 1103 90-91 0.6144241021682291 1103 92-93 0.7782530561686478 1103 94-95 0.6319388512663942 1103 96-97 0.6972546642102238 1103 98-99 0.8416546190305212 1103 100-101 0.9743180690521456 1104 1 -0.23462664300083347 1104 2 -0.08040611358620708 1104 3 -0.3573734110085738 1104 4 -0.147602929448432 1104 5 -0.24546907612629099 1104 6 -0.46696762317949236 1104 7 -0.20833017278073385 1104 8 -0.25494292795946905 1104 9 -0.36686500342948136 1104 10-11 -0.4098436695426031 1104 12-13 -0.1945658268402468 1104 14-15 -0.17424731266596183 1104 16-17 -0.08910755007570259 1104 18-19 0.014899444347229007 1104 20-21 -0.09958205799424746 1104 22-23 -0.08005862692375132 1104 24-25 0.054383279611307955 1104 26-27 -0.11749305922418074 1104 28-29 -0.05593082581943776 1104 30-31 -4.1371627877140327E-4 1104 32-33 -0.09305622089514998 1104 34-35 -0.18141507062492934 1104 36-37 0.20958755795865613 1104 38-39 0.28171930597284955 1104 40-41 -0.052779757925634385 1104 42-43 -0.07236181672847408 1104 44-45 0.37802236531644695 1104 46-47 0.17377292283232748 1104 48-49 0.2435135297142068 1104 50-51 -0.11195159234245722 1104 52-53 -0.00236854759995353 1104 54-55 0.023517048977190314 1104 56-57 0.23702890167837865 1104 58-59 0.42118728908747727 1104 60-61 0.3489637854161032 1104 62-63 0.49238932121929935 1104 64-65 0.5773825097032663 1104 66-67 0.573715096131437 1104 68-69 0.641163331969576 1104 70-71 0.15106249570382246 1104 72-73 0.43778388899609766 1104 74-75 0.3497251781337667 1104 76-77 0.24402094939795305 1104 78-79 0.2122026051712922 1104 80-81 0.3601257156638802 1104 82-83 0.5744671490829738 1104 84-85 0.4861930891110511 1104 86-87 0.569537822790533 1104 88-89 0.4316810547385437 1104 90-91 0.4433405608380241 1104 92-93 0.5697178435224366 1104 94-95 0.6133781895916535 1104 96-97 0.43083514676295565 1104 98-99 -0.09756135512892783 1104 100-101 0.4243376452279435 1105 1 0.23081553386991516 1105 2 0.4606142945770557 1105 3 0.40527965021591683 1105 4 0.41491407735428965 1105 5 0.47970099190091986 1105 6 0.29446094824908187 1105 7 0.1293569020491958 1105 8 0.36927475911536334 1105 9 0.05313499657052034 1105 10-11 0.2012447658315466 1105 12-13 0.29914165615294763 1105 14-15 0.04194316352451466 1105 16-17 -0.09757693783080867 1105 18-19 0.20751849196627603 1105 20-21 0.1620165814615362 1105 22-23 0.1382747064095753 1105 24-25 0.19530164695824936 1105 26-27 0.6460443557418145 1105 28-29 0.5723004666975697 1105 30-31 0.4407767599117065 1105 32-33 0.5263315342068893 1105 34-35 0.5085169021641889 1105 36-37 0.4446555851695422 1105 38-39 0.35369209508849764 1105 40-41 0.28215221486348696 1105 42-43 0.09029124449601511 1105 44-45 0.2950631816429734 1105 46-47 0.34343278677790323 1105 48-49 0.28742509434005825 1105 50-51 0.20930691105890276 1105 52-53 0.27885594219596754 1105 54-55 0.6640272530588263 1105 56-57 0.37723298331103194 1105 58-59 0.2441124591555095 1105 60-61 0.016548819429708317 1105 62-63 0.18670904911045838 1105 64-65 0.5872464552814911 1105 66-67 0.4353137355872221 1105 68-69 0.2888163931940646 1105 70-71 0.22820535284668253 1105 72-73 0.2523757257307899 1105 74-75 -0.13949250894106058 1105 76-77 0.3067760514387672 1105 78-79 0.6695835575522437 1105 80-81 0.533016872126467 1105 82-83 0.4606916388788882 1105 84-85 0.40673730679812437 1105 86-87 0.5230752377565189 1105 88-89 0.6711708506569103 1105 90-91 0.9020140302257786 1105 92-93 1.0847518571278805 1105 94-95 0.9079700263263462 1105 96-97 0.9063793644500286 1105 98-99 0.7502277605173333 1105 100-101 0.6230111146156965 1106 1 0.27291343596781914 1106 2 0.30977513373789733 1106 3 0.4104777854140522 1106 4 0.16705859949880875 1106 5 0.7403070525069779 1106 6 0.7210110647991925 1106 7 0.677189069881365 1106 8 0.5258714957120958 1106 9 0.44199280542832753 1106 10-11 0.3636923182790994 1106 12-13 0.28859387060516184 1106 14-15 0.663248524829875 1106 16-17 0.5258846006307252 1106 18-19 0.5397912192390066 1106 20-21 0.6207928052377625 1106 22-23 0.6501278532627239 1106 24-25 0.7331687798253839 1106 26-27 0.4632238429212947 1106 28-29 0.41798811238520983 1106 30-31 0.513655547790492 1106 32-33 0.2236508815262397 1106 34-35 0.4865122401595201 1106 36-37 0.5935250490390089 1106 38-39 0.556349437745844 1106 40-41 0.8039587416700087 1106 42-43 0.23453366873844317 1106 44-45 0.6117648133446068 1106 46-47 0.47261693596205134 1106 48-49 0.8790684509834179 1106 50-51 0.754586631338622 1106 52-53 0.7902079235479462 1106 54-55 0.7786309826625555 1106 56-57 0.7064404425184918 1106 58-59 0.8162802913233307 1106 60-61 1.062131570012454 1106 62-63 1.059774317175723 1106 64-65 0.79319051122555 1106 66-67 1.1822018474753335 1106 68-69 1.240016859394533 1106 70-71 1.0699769146182412 1106 72-73 1.1807789891340548 1106 74-75 1.1365098220612673 1106 76-77 0.7541536738163899 1106 78-79 0.5509471939158814 1106 80-81 0.805382839492431 1106 82-83 0.8215890747763233 1106 84-85 0.7861312461920669 1106 86-87 1.0162220909033728 1106 88-89 1.4737699182559787 1106 90-91 1.08680424001599 1106 92-93 1.261977964353985 1106 94-95 1.4799164132727327 1106 96-97 0.9947593178299847 1106 98-99 1.566952702242272 1106 100-101 1.2502022568068405 1107 1 0.6764581670987546 1107 2 0.6477302820378732 1107 3 0.57204036076973 1107 4 0.5409433354525106 1107 5 0.6854481183376961 1107 6 0.49422061387081584 1107 7 0.6660967139614229 1107 8 0.6377596076002092 1107 9 0.6169385493395936 1107 10-11 0.37315699153687376 1107 12-13 0.5148417606461564 1107 14-15 0.6493830799299474 1107 16-17 0.6731597392851754 1107 18-19 0.7576595577970053 1107 20-21 0.3531294341261102 1107 22-23 0.567538029293587 1107 24-25 0.6144082300303566 1107 26-27 0.5429200088243604 1107 28-29 0.3825408010758409 1107 30-31 0.19860852584692168 1107 32-33 -0.11008434876071505 1107 34-35 0.13493278513179519 1107 36-37 0.16544450888950024 1107 38-39 0.2920620010446058 1107 40-41 0.031593176200992446 1107 42-43 -0.22628137828350248 1107 44-45 -0.054680809997570634 1107 46-47 0.13273268228469703 1107 48-49 0.1775975081331609 1107 50-51 -0.2564454400382772 1107 52-53 -0.08482221872357343 1107 54-55 -0.022550594381094413 1107 56-57 -0.10987255482898917 1107 58-59 -0.17262735275672014 1107 60-61 -0.08604783678763539 1107 62-63 0.09865784743856665 1107 64-65 -0.14441498672477238 1107 66-67 -0.1514730982685819 1107 68-69 -0.4163612452594343 1107 70-71 -0.24283957923273292 1107 72-73 0.11356172364092743 1107 74-75 0.14850644612685926 1107 76-77 0.22268514234785997 1107 78-79 0.0807277581792043 1107 80-81 -0.19672711951407962 1107 82-83 -0.09497711765193273 1107 84-85 -0.06810072872956496 1107 86-87 -0.36670532650680343 1107 88-89 -0.4258563175771535 1107 90-91 -0.8518260951660679 1107 92-93 -0.5234090624123517 1107 94-95 -0.5961626800477404 1107 96-97 -0.600731398350387 1107 98-99 -0.8306238591273889 1107 100-101 -0.310745416209798 1108 1 0.10229701535139668 1108 2 -0.03555854492911692 1108 3 -0.03027590533963931 1108 4 -0.07422172511484604 1108 5 0.2167380289379537 1108 6 -0.16097115051635313 1108 7 0.0031840625430277214 1108 8 -0.2631943766871032 1108 9 0.1979498113853353 1108 10-11 0.02729414854759682 1108 12-13 -0.14036451668656014 1108 14-15 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1.2442956932100167 2316 86-87 1.0802726899858186 2316 88-89 1.3254914451367448 2316 90-91 1.6700076607990297 2316 92-93 1.951991772202188 2316 94-95 1.9953054828019319 2316 96-97 1.5026320183778452 2316 98-99 1.4028286097742324 2316 100-101 1.058117271728225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18886.0 3 1844.0 4 1409.0 5 1166.0 6 1110.0 7 1098.0 8 1048.0 9 1075.0 10 1084.0 11 1065.0 12 1135.0 13 1125.0 14 1226.0 15 1270.0 16 1531.0 17 1857.0 18 1666.0 19 1445.0 20 1457.0 21 1635.0 22 1719.0 23 1841.0 24 2260.0 25 2585.0 26 3084.0 27 3740.0 28 4489.0 29 5682.0 30 6743.0 31 8397.0 32 10601.0 33 13855.0 34 18513.0 35 26726.0 36 41159.0 37 68546.0 38 36408.0 39 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.001148143834136 20.17005840557765 12.424913057224154 24.403880393364062 2 16.437198188214072 21.188643390342687 38.49745571208445 23.876702709358785 3 19.864420091384986 26.89667107062868 28.479767843534 24.759140994452338 4 13.604006855583473 16.446411634524186 37.33264555635057 32.61693595354178 5 13.757849861722626 37.70695846354037 33.33499732097989 15.200194353757118 6 29.644777828066342 36.660365804957266 18.826708554085357 14.868147812891031 7 27.71025798035196 31.05881100077208 22.611551875615664 18.619379143260296 8 26.956816911157848 33.819986155853144 20.300977077287612 18.922219855701393 9 28.22941348739384 14.420875376054951 20.2194430393227 37.13026809722851 10-11 26.455343400307836 25.245476101335328 27.075169516202834 21.224010982154 12-13 26.71587550917175 23.454846782567024 26.938513351614706 22.890764356646525 14-15 23.88297881191645 24.725809591797002 24.901364670330857 26.489846925955696 16-17 23.147041889782646 26.6419616678703 26.43296203728048 23.778034405066578 18-19 22.701866196304216 27.041126207453136 27.56396069589237 22.693046900350275 20-21 23.971007161683662 27.054324964724046 26.22800244934906 22.746665424243233 22-23 24.467699209286227 26.251797076755146 26.03548228215436 23.245021431804265 24-25 23.74408253255127 26.372478056491534 26.688630974666168 23.19480843629103 26-27 23.819420409467266 26.706390937408482 26.25429301669285 23.219895636431403 28-29 23.056661164505737 26.786926599398313 27.22488086046697 22.93153137562898 30-31 23.789302734219003 26.594572828199464 26.89724714464471 22.718877292936824 32-33 23.6483653257368 27.100250259577752 26.152125875242938 23.09925853944251 34-35 23.412249407630252 26.862470381512736 26.799905487074362 22.925374723782646 36-37 23.711429408162722 25.949455552301586 26.619199435584783 23.719915603950906 38-39 23.83988711695642 26.104703016426612 26.694576821703365 23.360833044913605 40-41 23.73787797102028 25.210492335749418 27.46417470364676 23.58745498958354 42-43 23.757521099012273 25.52631053486329 26.985769814435184 23.73039855168925 44-45 23.37043427907743 26.202341868935875 27.071931684118923 23.355292167867773 46-47 23.063105030941724 25.838895247223792 27.11694849078451 23.981051231049975 48-49 23.567574619251985 25.524751547912533 27.053931915263707 23.853741917571774 50-51 22.775486201256665 25.927503763141758 26.954620102277264 24.342389933324313 52-53 22.371861007883165 26.077048671281037 26.892410325243155 24.65867999559264 54-55 22.652142125065517 25.862408567832567 26.94073225857067 24.544717048531243 56-57 23.199511392498007 25.52373450990732 26.76778127471823 24.508972822876444 58-59 22.226801921693813 25.803361796072842 27.25247512361017 24.717361158623174 60-61 22.911783302941778 25.874584375349308 26.560771706325838 24.652860615383076 62-63 22.996772014113056 25.67358139612475 26.190225959012086 25.13942063075011 64-65 22.123797846341198 25.819549750185587 26.72238487267389 25.33426753079932 66-67 23.07528072645267 25.636179851747077 26.263352437633024 25.02518698416723 68-69 23.04524964603113 25.42772280860332 26.284585968990825 25.242441576374723 70-71 22.573101756176808 25.431121474594732 26.380681221134168 25.615095548094292 72-73 23.161167314783608 25.24302289118846 26.674413885968313 24.921395908059623 74-75 23.43744512179825 25.710008947760134 26.13164078506142 24.720905145380197 76-77 23.173704016177197 25.380492279672705 26.153194595101308 25.292609109048787 78-79 23.261249949911175 25.620433568862115 26.18460069189362 24.933715789333082 80-81 23.408278454056617 25.726058672149588 26.419156400097464 24.446506473696335 82-83 23.72463366693475 24.897633643574864 26.963648680667024 24.414084008823362 84-85 23.57633373820424 25.07958382829914 26.46571054844436 24.878371885052257 86-87 23.409372956671426 25.256541987483157 26.821481471596897 24.51260358424852 88-89 24.21799026876815 24.35877120262613 26.870309734734693 24.552928793871025 90-91 24.247772552643827 24.380727096202705 26.479640605685518 24.89185974546795 92-93 24.594283617929563 24.205076040554964 26.57200506937033 24.628635272145143 94-95 24.079751277370743 24.10525701616197 27.099764113592222 24.715227592875063 96-97 24.51546254332178 24.052086110370567 26.56541588909624 24.86703545721141 98-99 24.252831616116673 24.20100683387241 26.37331506968017 25.172846480330747 100-101 24.546979949645 24.046929128085324 26.08095791141719 25.325133010852486 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6.0 1 5.0 2 6.5 3 7.0 4 3.5 5 4.0 6 9.0 7 16.5 8 24.5 9 28.5 10 34.5 11 36.0 12 32.5 13 29.0 14 37.5 15 49.5 16 63.0 17 79.5 18 94.0 19 118.0 20 138.5 21 140.0 22 147.0 23 195.5 24 225.5 25 238.0 26 261.5 27 289.5 28 314.5 29 390.0 30 479.0 31 547.0 32 704.0 33 899.5 34 1135.0 35 1509.0 36 2116.0 37 3303.5 38 4869.5 39 6729.0 40 8785.5 41 10735.5 42 12580.5 43 15002.5 44 17916.5 45 19886.5 46 21089.5 47 21544.0 48 20730.5 49 18590.0 50 16059.0 51 13984.0 52 11771.5 53 9913.5 54 8592.5 55 7238.5 56 6284.5 57 5285.5 58 4261.5 59 3827.5 60 3353.0 61 2708.0 62 2149.5 63 1736.0 64 1425.5 65 1112.0 66 900.5 67 785.5 68 693.5 69 632.0 70 564.5 71 503.0 72 457.5 73 416.5 74 373.0 75 341.5 76 324.0 77 304.5 78 272.0 79 247.5 80 242.0 81 222.5 82 194.0 83 176.5 84 157.5 85 128.0 86 118.5 87 105.0 88 91.5 89 86.0 90 67.0 91 52.5 92 48.0 93 39.5 94 26.0 95 13.5 96 11.0 97 10.5 98 4.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.983759750805355E-4 2 0.0036607119086286308 3 3.327919916935119E-4 4 9.983759750805355E-4 5 0.0016639599584675595 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.6639599584675595E-4 12-13 0.0 14-15 0.0041598998961688985 16-17 0.002995127925241607 18-19 0.0034943159127818753 20-21 0.0 22-23 0.0 24-25 1.6639599584675595E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.655839833870238E-4 42-43 0.0 44-45 4.991879875402678E-4 46-47 0.7003607465189958 48-49 0.5115012912329278 50-51 0.4005151620031415 52-53 0.3291312797848832 54-55 0.3158196001171428 56-57 0.2865339048481137 58-59 0.28503634088549296 60-61 0.2624064854503341 62-63 0.25475226964138337 64-65 0.25475226964138337 66-67 0.24293815393626367 68-69 0.22413540640558025 70-71 0.23877825404009478 72-73 0.7709126487580203 74-75 0.50933814328692 76-77 0.5976944170815474 78-79 0.3394478315273821 80-81 0.2965176645989191 82-83 0.27588456111392134 84-85 0.2675647613215836 86-87 0.2436037379196507 88-89 0.24343734192380392 90-91 0.2534211016746093 92-93 0.2156492106173957 94-95 0.18486595138574585 96-97 0.13577913261095287 98-99 0.14592928835760496 100-101 0.13927344852373474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 300488.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.489543672717154 #Duplication Level Percentage of deduplicated Percentage of total 1 90.52615164866063 57.47464058620655 2 4.8238023279493705 6.125220171377924 3 1.3615480851068273 2.593321998356829 4 0.6747119509853607 1.71348615514357 5 0.4413323937291661 1.4009996142926344 6 0.3071010807752838 1.169862448789261 7 0.2121950922811589 0.9430518704964617 8 0.16902981627869654 0.8585300722094128 9 0.14705372632040034 0.8402736581509368 >10 1.149852842250556 14.911229929160125 >50 0.13515675889121223 5.942287382142459 >100 0.05101247320018516 5.617743732345164 >500 0.0010518035711378384 0.4093523813286877 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 619 0.20599824285828386 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 607 0.20200473895796173 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 420 0.139772636511275 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 407 0.13544634061925934 No Hit CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC 377 0.12546258086845397 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 353 0.1174755730678097 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 339 0.11281648518410053 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC 332 0.11048694124224595 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 316 0.10516226937514975 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.6639599584675595E-4 0.0 16-17 0.0 0.0 0.0 6.655839833870238E-4 0.0 18-19 0.0 0.0 0.0 8.319799792337798E-4 0.0 20-21 0.0 0.0 0.0 0.0014975639626208037 0.0 22-23 0.0 0.0 0.0 0.003494315912781875 0.0 24-25 0.0 0.0 0.0 0.008319799792337799 0.0 26-27 0.0 0.0 0.0 0.01713878757221586 0.0 28-29 0.0 0.0 0.0 0.03827107904475387 0.0 30-31 0.0 0.0 0.0 0.11414765315087458 0.0 32-33 0.0 0.0 0.0 0.31948031202577143 0.0 34-35 0.0 0.0 0.0 0.642288543968478 0.0 36-37 0.0 0.0 0.0 1.1091957083144752 0.0 38-39 0.0 0.0 0.0 1.7100516493171107 0.0 40-41 0.0 0.0 0.0 2.3080788583903518 0.0 42-43 0.0 0.0 0.0 2.8944583477543198 0.0 44-45 0.0 0.0 0.0 3.5838369585474297 0.0 46-47 0.0 0.0 0.0 4.314148984318841 0.0 48-49 0.0 0.0 0.0 5.055609541811986 0.0 50-51 0.0 0.0 0.0 5.781595271691382 0.0 52-53 0.0 0.0 0.0 6.431538031468811 0.0 54-55 0.0 0.0 0.0 7.125076542158089 0.0 56-57 0.0 0.0 0.0 7.866537099651234 0.0 58-59 0.0 0.0 0.0 8.67821676739171 0.0 60-61 0.0 0.0 0.0 9.441641596336627 0.0 62-63 0.0 0.0 0.0 10.192420329597187 0.0 64-65 0.0 0.0 0.0 10.928057027235697 0.0 66-67 0.0 0.0 0.0 11.679334948483799 0.0 68-69 0.0 0.0 0.0 12.477536540560688 0.0 70-71 0.0 0.0 0.0 13.291212960251325 0.0 72-73 0.0 0.0 0.0 14.073939724714464 0.0 74-75 0.0 0.0 0.0 14.831374297808898 0.0 76-77 0.0 0.0 0.0 15.579823487127605 0.0 78-79 0.0 0.0 0.0 16.36704294347861 0.0 80-81 0.0 0.0 0.0 17.143113868107875 0.0 82-83 0.0 0.0 0.0 17.963945315619924 0.0 84-85 0.0 0.0 0.0 18.728035728548228 0.0 86-87 0.0 0.0 0.0 19.511594472990602 0.0 88-89 0.0 0.0 0.0 20.334755464444505 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 425 0.0 54.639706 3 TACCTGG 425 0.0 50.17932 2 GTACTAG 135 0.0 45.636337 1 TAAGTGT 45 9.722434E-4 42.125847 6 GTAAGAC 45 9.722434E-4 42.125847 3 CCTGGGG 420 0.0 39.49298 4 GTCTATA 85 3.6943493E-7 39.02836 1 GTACCTG 595 0.0 36.638866 1 CTGGGGG 450 0.0 33.70068 5 GCATCGT 190 0.0 32.425816 8 GATATCG 45 2.4212866E-5 31.64705 88-89 ATTAATA 135 1.2178134E-8 31.594387 2 GTATATA 80 4.5462628E-4 29.619738 1 ATATAAC 85 6.482119E-4 27.8774 3 TACGGGC 205 3.274181E-11 27.741411 4 GTATTAA 120 5.4605134E-6 27.645088 1 GGGCTAT 120 5.4605134E-6 27.645088 6 TATATAA 70 0.008475827 27.080904 2 TGTAGGC 70 0.008475827 27.080904 2 CATGGGT 105 6.997156E-5 27.0809 4 >>END_MODULE