FastQCFastQC Report
Sat 18 Jun 2016
SRR1931070_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931070_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300488
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT24240.8066877878650729No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT20820.6928729267058917RNA PCR Primer, Index 26 (100% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG13740.4572561965868853RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT9290.3091637602832725RNA PCR Primer, Index 26 (100% over 24bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT6430.21398525065892815No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG4890.1627352839381273No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4750.15807619605441814No Hit
CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC4560.15175314821224142No Hit
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT4350.14476451638667767RNA PCR Primer, Index 26 (100% over 27bp)
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4130.13744309256942042No Hit
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC4120.13711030057772688No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3930.13078725273555017No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT3490.11614440510103564No Hit
GCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCCTATTGGGA3310.11015414925055243No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3230.10749181331700433No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG3140.10449668539176274No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG3130.10416389340006922No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT3120.1038311014083757No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG3070.10216714144990816No Hit
GTACCTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCT3030.10083597348313411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGGG3500.062.4272585
ACCTGGG4500.062.2764663
CAGTACG701.8189894E-1161.0701459
TACCTGG4800.058.3939062
CCTGGGG4250.055.8811764
GTACCCG405.364701E-447.5306431
ACGATCG502.822323E-547.4994
GTACTAG1750.040.740551
ATTAATA1650.037.4296882
GTACCTG7550.037.1431471
CTAACGC905.680249E-736.943673
GGGTAGA651.3208277E-436.562031
TAACGCC801.0751179E-535.6242524
TCGGCTC702.04999E-433.927868
TGGGGGG8300.033.1920746
GCACGAT753.070458E-431.671272
TCACGGC600.003946755531.6660023
GCACTCC600.003946755531.6660026
TCAATAC753.073458E-431.6659983
ACAGTAC1303.0248157E-729.2301568
Produced by FastQC (version 0.11.5)