##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931070_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 300488 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.61868028007774 33.0 33.0 33.0 33.0 33.0 2 32.577067969436385 33.0 33.0 33.0 33.0 33.0 3 32.639499747078084 33.0 33.0 33.0 33.0 33.0 4 36.565426905566945 37.0 37.0 37.0 37.0 37.0 5 36.50573067809696 37.0 37.0 37.0 37.0 37.0 6 36.511311599797665 37.0 37.0 37.0 37.0 37.0 7 36.452650355421845 37.0 37.0 37.0 37.0 37.0 8 36.45345238412183 37.0 37.0 37.0 37.0 37.0 9 36.450404009477914 37.0 37.0 37.0 37.0 37.0 10-11 36.42484558451585 37.0 37.0 37.0 37.0 37.0 12-13 36.30921201512207 37.0 37.0 37.0 35.0 37.0 14-15 38.186004099997334 40.0 37.0 40.0 33.0 40.0 16-17 38.15080801895583 40.0 37.0 40.0 33.0 40.0 18-19 38.097792590719095 40.0 37.0 40.0 33.0 40.0 20-21 38.01617701871622 40.0 37.0 40.0 33.0 40.0 22-23 37.85783625302841 40.0 37.0 40.0 33.0 40.0 24-25 38.020290327733555 40.0 37.0 40.0 33.0 40.0 26-27 37.948227549852234 40.0 37.0 40.0 33.0 40.0 28-29 37.83501171427811 40.0 37.0 40.0 33.0 40.0 30-31 37.729633130108354 40.0 37.0 40.0 33.0 40.0 32-33 37.564501743830036 40.0 37.0 40.0 33.0 40.0 34-35 37.473601275258915 40.0 37.0 40.0 33.0 40.0 36-37 37.42762273368653 40.0 37.0 40.0 33.0 40.0 38-39 37.240758366390665 40.0 37.0 40.0 33.0 40.0 40-41 37.04545106626554 40.0 37.0 40.0 33.0 40.0 42-43 36.83248748702111 40.0 37.0 40.0 33.0 40.0 44-45 36.62588356273795 40.0 37.0 40.0 33.0 40.0 46-47 36.36161344213413 37.0 37.0 40.0 33.0 40.0 48-49 36.164697092729156 37.0 37.0 40.0 33.0 40.0 50-51 35.852027368813395 37.0 37.0 40.0 30.0 40.0 52-53 35.646168898591625 37.0 37.0 40.0 27.0 40.0 54-55 35.38430985596763 37.0 37.0 40.0 27.0 40.0 56-57 34.934098865844895 37.0 33.0 40.0 27.0 40.0 58-59 34.55699728441735 37.0 33.0 40.0 27.0 40.0 60-61 34.20057206943372 37.0 33.0 40.0 27.0 40.0 62-63 33.78295639093741 37.0 33.0 38.5 27.0 40.0 64-65 33.270132251537504 37.0 33.0 37.0 22.0 40.0 66-67 32.74645576528847 37.0 33.0 37.0 22.0 40.0 68-69 32.315253853731264 37.0 33.0 37.0 22.0 40.0 70-71 31.969433055562952 37.0 33.0 37.0 15.0 40.0 72-73 31.622702071297358 37.0 33.0 37.0 15.0 37.0 74-75 31.22077087936956 35.0 33.0 37.0 15.0 37.0 76-77 29.570561885998774 33.0 27.0 37.0 15.0 37.0 78-79 30.333767072229172 33.0 33.0 37.0 15.0 37.0 80-81 30.36881339687442 33.0 33.0 37.0 6.0 37.0 82-83 30.123668832033225 33.0 33.0 37.0 6.0 37.0 84-85 29.811974188653124 33.0 27.0 37.0 4.0 37.0 86-87 29.462294667341126 33.0 27.0 37.0 2.0 37.0 88-89 29.11684659620351 33.0 27.0 37.0 2.0 37.0 90-91 28.690500119805115 33.0 27.0 37.0 2.0 37.0 92-93 28.257517771092356 33.0 27.0 37.0 2.0 37.0 94-95 27.745911650382045 33.0 27.0 37.0 2.0 37.0 96-97 27.19960530869785 33.0 27.0 37.0 2.0 37.0 98-99 26.464735696600197 33.0 22.0 33.0 2.0 37.0 100-101 24.739507068501904 33.0 18.5 33.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15404933956610734 1101 2 0.2114301327473811 1101 3 0.2917886905349931 1101 4 0.2909158575736086 1101 5 0.0422056629285521 1101 6 0.11043360776567113 1101 7 0.06950719121639537 1101 8 0.14769902096630005 1101 9 0.021285205842971777 1101 10-11 0.1573604582924375 1101 12-13 0.13588350697397544 1101 14-15 0.23670487735784462 1101 16-17 0.23864518728660045 1101 18-19 0.2204903516132788 1101 20-21 0.018280116605218666 1101 22-23 -0.0364042746023685 1101 24-25 0.010062617433888477 1101 26-27 0.2306555847780274 1101 28-29 0.35606672553246455 1101 30-31 0.3398959142773137 1101 32-33 0.21158944530571944 1101 34-35 0.33638278606515115 1101 36-37 -0.005662079446516088 1101 38-39 -0.40582004704346275 1101 40-41 -0.15197570405479865 1101 42-43 -0.26715374050250773 1101 44-45 -0.2010146083956954 1101 46-47 -0.4245964435959806 1101 48-49 -0.5523502796377215 1101 50-51 -0.6390028491124866 1101 52-53 -0.5963384995688656 1101 54-55 -0.5642462012173013 1101 56-57 -0.37198152897530434 1101 58-59 -0.47999327498613553 1101 60-61 -0.3124696184127558 1101 62-63 -0.7690852482188575 1101 64-65 -1.0326658155491728 1101 66-67 -0.45685898324551033 1101 68-69 -0.3846580135786226 1101 70-71 -0.880657171283687 1101 72-73 -0.7854952070872763 1101 74-75 -0.6986743873373662 1101 76-77 -0.9876530497879727 1101 78-79 -1.0382411484513874 1101 80-81 -1.0721636739698255 1101 82-83 -1.7225457166279945 1101 84-85 -1.4150398346633963 1101 86-87 -1.4157668143207793 1101 88-89 -1.4158517704820817 1101 90-91 -2.2204732511957097 1101 92-93 -1.6590518269060013 1101 94-95 -1.6390408593140435 1101 96-97 -2.3118568741467023 1101 98-99 -2.0304304238695323 1101 100-101 -3.8076039842273666 1102 1 -0.08273515039442714 1102 2 0.015566911475808354 1102 3 0.09383691454328869 1102 4 -0.1577633116137278 1102 5 -0.032146885108204515 1102 6 0.012660439212105246 1102 7 0.04836237515325337 1102 8 -0.017743320519599592 1102 9 0.04597336301751653 1102 10-11 0.12917217148345372 1102 12-13 0.0034691377175022353 1102 14-15 0.11180930509384979 1102 16-17 -0.10386338298966535 1102 18-19 -0.036495503829925724 1102 20-21 -0.2099557028284238 1102 22-23 -0.1292441328687275 1102 24-25 0.2573907077746753 1102 26-27 0.1293462770258884 1102 28-29 0.19930989091719908 1102 30-31 0.19151490428105689 1102 32-33 0.06701824719549876 1102 34-35 -0.23368462383892563 1102 36-37 -0.35604954235666497 1102 38-39 0.044861253910070786 1102 40-41 -0.7045064562225818 1102 42-43 -0.45631198093116865 1102 44-45 -0.7847800564987182 1102 46-47 -0.9678727377797145 1102 48-49 -0.9672524776615106 1102 50-51 -0.976902195178809 1102 52-53 -1.6226182794784094 1102 54-55 -1.2385685012201861 1102 56-57 -1.345809777758177 1102 58-59 -0.9676275906603777 1102 60-61 -0.8595413206568665 1102 62-63 -0.9331952358315689 1102 64-65 -1.082423831278149 1102 66-67 -1.0241464547979575 1102 68-69 -1.3361531306817263 1102 70-71 -1.6545099065701834 1102 72-73 -1.7557945185844126 1102 74-75 -1.2758140756385785 1102 76-77 -1.466176945734734 1102 78-79 -1.6590676399675353 1102 80-81 -1.5366870513788662 1102 82-83 -1.441937587108793 1102 84-85 -1.7471776504672754 1102 86-87 -1.2584842400690555 1102 88-89 -1.713845317568186 1102 90-91 -1.753846915240878 1102 92-93 -1.760483567176216 1102 94-95 -1.6985430630993683 1102 96-97 -1.9615630361033176 1102 98-99 -2.730708417704694 1102 100-101 -3.578194253002753 1103 1 0.008193610356372005 1103 2 0.19726215678332437 1103 3 0.20967507108572647 1103 4 0.17657974026056422 1103 5 0.1629091001787515 1103 6 0.1060233428741384 1103 7 0.21007060255629284 1103 8 0.12530314456397207 1103 9 0.12462322508275037 1103 10-11 0.18544144178149935 1103 12-13 0.17345345101873733 1103 14-15 0.450533814208363 1103 16-17 0.33337629894899123 1103 18-19 0.11502989239444616 1103 20-21 -0.03285828301991245 1103 22-23 0.03998289152679746 1103 24-25 -0.12380959514088374 1103 26-27 -0.2575607387149006 1103 28-29 -0.04706594074830406 1103 30-31 0.21185352060604146 1103 32-33 0.20457436771262394 1103 34-35 0.14728578780168533 1103 36-37 -0.09915280410816507 1103 38-39 0.015029480231270043 1103 40-41 -0.28402179132292105 1103 42-43 -0.004832562961787801 1103 44-45 -0.08431486474234617 1103 46-47 -0.42581615748004253 1103 48-49 -0.0010464950791586602 1103 50-51 -0.3943847504879372 1103 52-53 0.002699511401672794 1103 54-55 0.0700808199243994 1103 56-57 -0.27352925631068103 1103 58-59 -0.4555921062659465 1103 60-61 0.0441071891067466 1103 62-63 -0.26916104964668364 1103 64-65 -0.22043973434516317 1103 66-67 0.0781957316032944 1103 68-69 -0.20527738015432817 1103 70-71 -0.3381118971581607 1103 72-73 0.049257680258019576 1103 74-75 -0.4920741502856316 1103 76-77 -0.4210235947313272 1103 78-79 -0.38671409423269054 1103 80-81 -0.23085051759836972 1103 82-83 -0.32081193124250973 1103 84-85 -0.6732608440627743 1103 86-87 -0.5238734324745309 1103 88-89 -1.3424897704269512 1103 90-91 -1.3693334733628006 1103 92-93 -0.8116952294253643 1103 94-95 -0.5631911942185326 1103 96-97 -0.47190814347606747 1103 98-99 -0.98126286136781 1103 100-101 -1.3141573622146332 1104 1 0.09180835121674846 1104 2 0.3098741080386418 1104 3 0.0988494458716147 1104 4 0.29873507721548975 1104 5 0.1125004475090492 1104 6 0.01230240126007942 1104 7 -0.0812473634790436 1104 8 0.110792304256627 1104 9 0.11292646004580575 1104 10-11 -0.05450129790430225 1104 12-13 0.16082681763157325 1104 14-15 0.24804274583857477 1104 16-17 0.2729715615144457 1104 18-19 0.04442107332724987 1104 20-21 0.14790323021848195 1104 22-23 0.07009349692003042 1104 24-25 0.22301906438048036 1104 26-27 0.21276911984723057 1104 28-29 0.08939223964616616 1104 30-31 -0.010776538611885655 1104 32-33 0.16189830448157494 1104 34-35 0.22988501454274513 1104 36-37 0.06903101618253515 1104 38-39 0.258520401779748 1104 40-41 0.2782182125923143 1104 42-43 0.6849339911418681 1104 44-45 0.2879016477619345 1104 46-47 0.5421523248769304 1104 48-49 0.4343765950201899 1104 50-51 0.21876554560171968 1104 52-53 0.20489693713455637 1104 54-55 0.2145285270648145 1104 56-57 0.44518556774843887 1104 58-59 0.4693577297835887 1104 60-61 0.8275335092750993 1104 62-63 0.5948212339142174 1104 64-65 0.701500264676028 1104 66-67 0.8535835845862181 1104 68-69 0.5631486953118312 1104 70-71 0.5214657468490813 1104 72-73 0.6173705369114693 1104 74-75 0.5866098412966139 1104 76-77 0.6929709239394519 1104 78-79 0.5263065096923292 1104 80-81 0.7611110152161942 1104 82-83 0.42621493705877 1104 84-85 0.7441399291961694 1104 86-87 0.6707137549184132 1104 88-89 0.41218404154387756 1104 90-91 0.4312587059549706 1104 92-93 0.9043034002423305 1104 94-95 1.0314963210753518 1104 96-97 0.8115304118021562 1104 98-99 0.871242889133832 1104 100-101 0.03951072372478137 1105 1 0.02044780699905857 1105 2 0.2715067610998716 1105 3 0.08408754110971017 1105 4 0.14118405680732593 1105 5 0.1799834407063301 1105 6 0.3222343740491951 1105 7 0.23126964332367805 1105 8 0.20616645391648802 1105 9 0.09136183419546029 1105 10-11 0.20941026672155516 1105 12-13 0.25143906252953485 1105 14-15 0.25726043291340517 1105 16-17 0.2788559152559458 1105 18-19 0.27050950870140156 1105 20-21 0.0761345227354866 1105 22-23 0.26618193229418097 1105 24-25 0.2345836902308207 1105 26-27 0.29600041236422925 1105 28-29 0.09660312399990545 1105 30-31 0.4126928491432196 1105 32-33 0.3304017058421138 1105 34-35 0.38212991250193085 1105 36-37 0.7537248937335619 1105 38-39 0.5987584970178403 1105 40-41 0.8317216139528583 1105 42-43 0.6579612020262218 1105 44-45 0.7209968858571756 1105 46-47 0.8531047058293098 1105 48-49 0.6815194521630517 1105 50-51 0.8764186068262063 1105 52-53 0.772243875910064 1105 54-55 1.141807438629435 1105 56-57 1.0503556357756523 1105 58-59 1.0795277978107976 1105 60-61 0.7216491555336049 1105 62-63 -0.023069922548359045 1105 64-65 0.4302077476692219 1105 66-67 0.9967808634977828 1105 68-69 1.2574684232029867 1105 70-71 1.0987106448082642 1105 72-73 0.8795814212652111 1105 74-75 1.2358275283714448 1105 76-77 0.7332090191775471 1105 78-79 1.1387895028896118 1105 80-81 1.2018593145359198 1105 82-83 1.170806773793462 1105 84-85 1.363561697903652 1105 86-87 1.1949994692041237 1105 88-89 1.4442248578704096 1105 90-91 1.6207144882678968 1105 92-93 1.2347795907185208 1105 94-95 1.7904418993066464 1105 96-97 1.340441976428007 1105 98-99 1.4247803040998193 1105 100-101 0.258660383588726 1106 1 0.10536622191747114 1106 2 0.2026955722886825 1106 3 0.056558403580567074 1106 4 0.07852671414998724 1106 5 0.10902772975061481 1106 6 0.19421572603054926 1106 7 0.09005752211155738 1106 8 0.05315946090949808 1106 9 0.17105880389242856 1106 10-11 0.26054080285208414 1106 12-13 0.027103398193865758 1106 14-15 0.23107162172458828 1106 16-17 0.2175388989389333 1106 18-19 0.22646521965711486 1106 20-21 0.23487578147674526 1106 22-23 0.3919977831100354 1106 24-25 0.42770723335436855 1106 26-27 0.47107034243416734 1106 28-29 0.5379317953285749 1106 30-31 0.44707513352549455 1106 32-33 0.5375345729749839 1106 34-35 0.16439098476300273 1106 36-37 0.41910950911817224 1106 38-39 0.7039915972509405 1106 40-41 0.6421994694307145 1106 42-43 0.512285211350239 1106 44-45 0.5213348811951732 1106 46-47 0.5961816289062298 1106 48-49 0.6458318064754067 1106 50-51 0.6288078842154903 1106 52-53 0.4685142721804638 1106 54-55 0.46425499107699153 1106 56-57 0.47525074067075934 1106 58-59 0.4551338584168576 1106 60-61 0.6766724655569121 1106 62-63 0.77194173246329 1106 64-65 1.080347607809081 1106 66-67 1.1987622154791353 1106 68-69 1.2430045537391194 1106 70-71 1.2304472415448657 1106 72-73 1.4907352674190584 1106 74-75 1.5492424467863657 1106 76-77 1.0720085529770778 1106 78-79 1.6393839084840174 1106 80-81 1.2501576828342884 1106 82-83 1.5107018786885682 1106 84-85 1.865578014919972 1106 86-87 1.3348829190875762 1106 88-89 1.4693530629986178 1106 90-91 1.4739778915312982 1106 92-93 1.0196047655436935 1106 94-95 1.4968405007052468 1106 96-97 1.7769221629081962 1106 98-99 2.106388695708212 1106 100-101 1.5809098008381426 1107 1 0.1345439407503619 1107 2 0.24417133790237955 1107 3 0.11624010119329853 1107 4 0.035604119503254594 1107 5 -0.05880443808155178 1107 6 0.30200448899172727 1107 7 0.2750105001679799 1107 8 0.2483608112832627 1107 9 0.1804631926071636 1107 10-11 0.03109783202980765 1107 12-13 0.07388420359536241 1107 14-15 0.10260526049960816 1107 16-17 0.24508893510965635 1107 18-19 0.2422597699344209 1107 20-21 0.1814636763405062 1107 22-23 0.27812028130149713 1107 24-25 0.4694844216832763 1107 26-27 0.41874335907269966 1107 28-29 0.2749103340312473 1107 30-31 0.27423934862074617 1107 32-33 0.255824903334279 1107 34-35 0.3269783973504161 1107 36-37 0.12269041097493982 1107 38-39 0.08547218562807757 1107 40-41 0.027055992218272706 1107 42-43 0.2244868028621667 1107 44-45 0.4975224866931143 1107 46-47 0.32200752714592085 1107 48-49 0.40933554411707007 1107 50-51 0.294469808498107 1107 52-53 0.49962109639073304 1107 54-55 0.17121182734416607 1107 56-57 0.1736471718258059 1107 58-59 0.4151652063792426 1107 60-61 0.6641935651469808 1107 62-63 0.9510471098445308 1107 64-65 0.963154456133168 1107 66-67 0.8041998812616313 1107 68-69 1.1628863960347573 1107 70-71 0.918715869468663 1107 72-73 0.6453651203247723 1107 74-75 1.0075255430004972 1107 76-77 0.8482926137438973 1107 78-79 1.254165678438202 1107 80-81 1.4518697638567168 1107 82-83 1.5609791875865646 1107 84-85 1.6572346341627942 1107 86-87 1.7805062612626408 1107 88-89 1.9998570417784585 1107 90-91 2.2379036209742686 1107 92-93 2.4168799041981437 1107 94-95 2.4534356297141713 1107 96-97 2.055436751767612 1107 98-99 2.658353971811419 1107 100-101 3.1521661307151625 1108 1 -0.03486083497625003 1108 2 -0.08380188087544127 1108 3 0.030507294196127077 1108 4 0.08785072347399137 1108 5 0.11010689749645053 1108 6 -0.028135996321175583 1108 7 -0.010952578898546506 1108 8 -0.0093891016390657 1108 9 -0.023946807779843482 1108 10-11 -0.020960103648818063 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0.4038456551899543 2316 56-57 0.46908175042533173 2316 58-59 0.6912369273224712 2316 60-61 0.5603858858945401 2316 62-63 0.39134621333273856 2316 64-65 0.4526918241023452 2316 66-67 0.8621736448141277 2316 68-69 0.8598144953898199 2316 70-71 0.8163008783539141 2316 72-73 0.9327979817747654 2316 74-75 1.0379400549107203 2316 76-77 1.0663406539970772 2316 78-79 1.2194582926985298 2316 80-81 1.2645557051940948 2316 82-83 1.319819512646962 2316 84-85 1.2534449250798723 2316 86-87 1.158354033959963 2316 88-89 1.1156049003332562 2316 90-91 1.3546847642763886 2316 92-93 1.4339515652408146 2316 94-95 1.892257185930852 2316 96-97 2.0686797683600666 2316 98-99 2.5571369919979077 2316 100-101 3.3950828888965816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4.0 5 9.0 6 18.0 7 28.0 8 82.0 9 143.0 10 317.0 11 444.0 12 703.0 13 984.0 14 1146.0 15 1384.0 16 1600.0 17 1848.0 18 2107.0 19 2034.0 20 2387.0 21 2652.0 22 2770.0 23 3022.0 24 3287.0 25 3210.0 26 3127.0 27 3693.0 28 4567.0 29 5603.0 30 7005.0 31 8807.0 32 11462.0 33 15146.0 34 22021.0 35 32973.0 36 53988.0 37 78903.0 38 23013.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.4440403394482 21.73740724458122 13.915977245780212 25.902575170190374 2 14.193292887878679 21.892794031022973 39.01133826088982 24.902574820208528 3 17.457602300258245 27.545525944463673 30.683088842150102 24.31378291312798 4 12.161550544447698 18.26062937621469 35.89494422406219 33.68287585527542 5 12.476371768589761 38.457775352093925 34.18272942679907 14.88312345251724 6 29.412821809855966 37.23709432656213 18.443997763637814 14.90608609994409 7 26.310202071297358 32.191302148505095 22.070764889113708 19.427730891083836 8 26.620696999547405 34.72884108516813 20.646747956657173 18.003713958627298 9 26.47460131519395 14.567969436383482 19.656026197385586 39.301403051036985 10-11 25.51965469502942 25.372727030696733 27.420396155586914 21.687222118686936 12-13 26.000039935039005 23.24868214371289 27.05682090466175 23.694457016586355 14-15 23.479678855193644 24.992220974250177 24.23827946254004 27.289820708016137 16-17 23.01905566944437 27.201086233060888 25.62614813237134 24.153709965123397 18-19 22.416702164479112 26.977616410638696 27.233533452251006 23.372147972631186 20-21 23.072801576102872 26.88543302893959 25.949621948297434 24.0921434466601 22-23 23.968677617741807 26.768955831846863 25.90685817736482 23.35550837304651 24-25 23.44419743883283 27.05083064881127 26.417693884614362 23.08727802774154 26-27 22.87179521311999 27.14983626634009 26.6747756981976 23.303592822342324 28-29 21.928995500652274 26.40737733287186 28.242392375069887 23.42123479140598 30-31 23.07296797209872 26.315859535156143 27.214231516732777 23.396940976012353 32-33 23.69978168845345 26.845830781928065 26.785429035435694 22.668958494182796 34-35 22.72569952876654 27.441029259071907 27.504925321477064 22.32834589068449 36-37 22.596409839993612 26.02000745454061 27.136690982668192 24.246891722797585 38-39 23.05183568062618 25.637795852080615 27.498768669630735 23.81159979766247 40-41 22.48380499769534 25.017929735405364 28.779596543513097 23.718668723386198 42-43 23.213349685411096 25.077419988251798 28.448288772812063 23.260941553525043 44-45 21.881239849844253 26.10536860041 28.21310667980086 23.80028486994489 46-47 22.70356886131892 25.155413860120866 27.648025877905276 24.492991400654933 48-49 22.814554990548707 24.876201379090013 28.13589893772796 24.173344692633318 50-51 21.747790261175155 25.49203295971886 28.358536780170922 24.401639998935064 52-53 21.480724687841114 25.987061047362957 28.40679161896648 24.12542264582945 54-55 21.54445435425042 26.11917946806528 27.888634487899683 24.447731689784614 56-57 22.642834322834858 26.01967466254892 26.97545326269269 24.362037751923538 58-59 20.730278746572242 27.445355554963925 27.888634487899683 23.93573121056415 60-61 21.88040786986502 28.145549905487073 26.02050664252815 23.953535582119752 62-63 21.508679215143367 28.5057972364953 25.39552328212774 24.590000266233595 64-65 20.62578206118048 28.436576502223048 26.087564228854397 24.85007720774207 66-67 21.47836056462711 27.837487499230402 25.769972527426816 24.91417940871568 68-69 21.550299619422596 27.13382113361146 25.7997201000451 25.516159146920852 70-71 21.257003273047363 26.878424677479206 25.91448144579837 25.950090603675065 72-73 21.697705066425282 26.52252336199782 26.0359814701419 25.743790101435 74-75 21.709186390138708 26.99109448630228 25.65393626367775 25.645782859881262 76-77 21.537859941926733 26.56627257826995 25.705323937334125 26.190543542469197 78-79 21.53988494710795 26.718429929061255 25.540610475903623 26.201074647927168 80-81 21.206448413688904 26.719016763346282 25.88567510990858 26.18885971305624 82-83 21.64655961394459 25.7174473749896 26.531158998252767 26.104834012813043 84-85 21.5975745514268 25.962749662623697 25.927972031462943 26.511703754486565 86-87 21.234564614383675 25.993846645356776 26.389039108246127 26.38254963201342 88-89 21.621994888315008 25.14509730837837 26.33782380660792 26.895083996698705 90-91 21.509344799126755 24.949914805250124 26.159114507068505 27.381625888554616 92-93 21.60204069747126 24.81351013609642 26.51192819620248 27.072520970229846 94-95 20.72511614904352 24.708628975359098 27.31249750396038 27.253757371637004 96-97 21.0812088052889 24.766830239101967 26.774294999475835 27.377665956133303 98-99 20.821630148292112 25.02462660738532 26.610713239796596 27.54303000452597 100-101 18.97949165938683 26.222887807313118 26.470152668580223 28.32746786471983 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8.0 1 7.0 2 4.5 3 6.0 4 7.5 5 5.5 6 10.5 7 18.5 8 24.0 9 35.0 10 32.0 11 25.0 12 28.0 13 29.0 14 35.5 15 51.5 16 62.0 17 69.0 18 82.5 19 90.5 20 102.5 21 110.5 22 122.5 23 143.0 24 155.0 25 177.0 26 194.0 27 201.5 28 230.0 29 298.5 30 376.0 31 436.0 32 566.5 33 785.0 34 1008.5 35 1359.0 36 1986.0 37 3149.0 38 4776.0 39 6812.5 40 8867.0 41 10790.0 42 12735.5 43 14889.0 44 17657.0 45 20368.5 46 21946.5 47 22485.5 48 22359.5 49 20889.0 50 18461.0 51 15733.5 52 13009.5 53 10699.0 54 9061.0 55 7645.0 56 6358.5 57 5062.5 58 3912.5 59 3502.0 60 2962.0 61 2117.0 62 1480.5 63 1166.0 64 856.5 65 510.0 66 325.0 67 241.0 68 192.5 69 154.5 70 112.0 71 82.0 72 61.5 73 41.5 74 38.5 75 30.5 76 19.0 77 13.0 78 11.0 79 7.5 80 4.0 81 3.0 82 2.5 83 2.5 84 2.0 85 1.0 86 1.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07853891003966881 2 3.327919916935119E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.6639599584675595E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0031615239210883626 42-43 0.005491067862942946 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002163147946007827 68-69 0.007820611804797529 70-71 0.0011647719709272915 72-73 0.0 74-75 0.0 76-77 0.0018303559543143154 78-79 0.005823859854636458 80-81 0.0036607119086286308 82-83 0.004326295892015654 84-85 0.002495939937701339 86-87 4.991879875402678E-4 88-89 0.0 90-91 0.0 92-93 8.319799792337797E-4 94-95 0.003993503900322142 96-97 0.0031615239210883626 98-99 0.0 100-101 1.6639599584675595E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 300488.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.08505784910566 #Duplication Level Percentage of deduplicated Percentage of total 1 88.42305412067097 49.59212105552432 2 5.82219216843274 6.530759691503203 3 1.6487302912138784 2.7740740128090953 4 0.8583709226554914 1.9256713141249375 5 0.533333162611636 1.4956010638960036 6 0.3939249667724359 1.3255982729783455 7 0.2937494850657189 1.153246980413909 8 0.2157296573985915 0.9679368251974199 9 0.1482661640483391 0.7483964748944478 >10 1.4061799127010421 16.339828108427486 >50 0.17375636042065679 6.693105923656614 >100 0.07973750786427401 7.966648292376317 >500 0.0011901120576757315 0.5246353783090599 >1k 0.0017851680865135972 1.9623766058888503 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 2424 0.8066877878650729 No Hit ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT 2082 0.6928729267058917 RNA PCR Primer, Index 26 (100% over 22bp) TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG 1374 0.4572561965868853 RNA PCR Primer, Index 26 (100% over 29bp) ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT 929 0.3091637602832725 RNA PCR Primer, Index 26 (100% over 24bp) GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 643 0.21398525065892815 No Hit GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG 489 0.1627352839381273 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 475 0.15807619605441814 No Hit CATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCC 456 0.15175314821224142 No Hit CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT 435 0.14476451638667767 RNA PCR Primer, Index 26 (100% over 27bp) CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 413 0.13744309256942042 No Hit GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC 412 0.13711030057772688 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 393 0.13078725273555017 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 349 0.11614440510103564 No Hit GCATGATGGTGATTGTTTTGAAAACCATAGGAGGAAACCTCCTATTGGGA 331 0.11015414925055243 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 323 0.10749181331700433 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 314 0.10449668539176274 No Hit TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG 313 0.10416389340006922 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 312 0.1038311014083757 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 307 0.10216714144990816 No Hit GTACCTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCT 303 0.10083597348313411 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.6639599584675595E-4 0.0 16-17 0.0 0.0 0.0 6.655839833870238E-4 0.0 18-19 0.0 0.0 0.0 8.319799792337798E-4 0.0 20-21 0.0 0.0 0.0 0.0014975639626208037 0.0 22-23 0.0 0.0 0.0 0.003494315912781875 0.0 24-25 0.0 0.0 0.0 0.007987007800644284 0.0 26-27 0.0 0.0 0.0 0.01680599558052235 0.0 28-29 0.0 0.0 0.0 0.037938287053060356 0.0 30-31 0.0 0.0 0.0 0.11314927717579404 0.0 32-33 0.0 0.0 0.0 0.3188147280423844 0.0 34-35 0.0 0.0 0.0 0.6426213359601715 0.0 36-37 0.0 0.0 0.0 1.1111924602646361 0.0 38-39 0.0 0.0 0.0 1.7117156092755783 0.0 40-41 0.0 0.0 0.0 2.308578046377892 0.0 42-43 0.0 0.0 0.0 2.8967878916961745 0.0 44-45 0.0 0.0 0.0 3.587497670456058 0.0 46-47 0.0 0.0 0.0 4.317476904235777 0.0 48-49 0.0 0.0 0.0 5.058271877745534 0.0 50-51 0.0 0.0 0.0 5.782260855674769 0.0 52-53 0.0 0.0 0.0 6.429208487526957 0.0 54-55 0.0 0.0 0.0 7.115758366390671 0.0 56-57 0.0 0.0 0.0 7.847401560128857 0.0 58-59 0.0 0.0 0.0 8.652092596043769 0.0 60-61 0.0 0.0 0.0 9.40653204121296 0.0 62-63 0.0 0.0 0.0 10.156312398498443 0.0 64-65 0.0 0.0 0.0 10.890784324166024 0.0 66-67 0.0 0.0 0.0 11.640398285455658 0.0 68-69 0.0 0.0 0.0 12.43177764170283 0.0 70-71 0.0 0.0 0.0 13.233806341684193 0.0 72-73 0.0 0.0 0.0 14.005883762413141 0.0 74-75 0.0 0.0 0.0 14.756496099677857 0.0 76-77 0.0 0.0 0.0 15.495294321237454 0.0 78-79 0.0 0.0 0.0 16.27003407789995 0.0 80-81 0.0 0.0 0.0 17.03379169883656 0.0 82-83 0.0 0.0 0.0 17.84114507068502 0.0 84-85 0.0 0.0 0.0 18.589261468012033 0.0 86-87 0.0 0.0 0.0 19.359342136790822 0.0 88-89 0.0 0.0 0.0 20.170023428556213 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGGGGG 350 0.0 62.427258 5 ACCTGGG 450 0.0 62.276466 3 CAGTACG 70 1.8189894E-11 61.070145 9 TACCTGG 480 0.0 58.393906 2 CCTGGGG 425 0.0 55.881176 4 GTACCCG 40 5.364701E-4 47.530643 1 ACGATCG 50 2.822323E-5 47.499 4 GTACTAG 175 0.0 40.74055 1 ATTAATA 165 0.0 37.429688 2 GTACCTG 755 0.0 37.143147 1 CTAACGC 90 5.680249E-7 36.94367 3 GGGTAGA 65 1.3208277E-4 36.56203 1 TAACGCC 80 1.0751179E-5 35.624252 4 TCGGCTC 70 2.04999E-4 33.92786 8 TGGGGGG 830 0.0 33.192074 6 GCACGAT 75 3.070458E-4 31.67127 2 TCACGGC 60 0.0039467555 31.666002 3 GCACTCC 60 0.0039467555 31.666002 6 TCAATAC 75 3.073458E-4 31.665998 3 ACAGTAC 130 3.0248157E-7 29.230156 8 >>END_MODULE