FastQCFastQC Report
Sat 18 Jun 2016
SRR1931069_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931069_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742779
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA39980.2294037281835505No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA35880.20587808322225595No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC30660.17592592061299797No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC26770.15360524771069656No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC26480.15194123867684886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25390.14568685989445593No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC25270.14499830443217412No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT22190.1273253809002748No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA21120.12118576136159546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.11407068825135028No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC19520.11200502186450492No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18810.10793106871267097No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT18750.10758679098153007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG31450.061.4233632
GTACCTG34150.056.844341
ACCTGGG35750.054.5651363
CCTGGGG32000.048.2349364
CTGGGGG26850.044.2612345
GTACCCG3550.042.6791461
GTACACG2750.041.321171
TGGGGGG47200.033.804116
TATCACG1553.8198777E-1133.6012232
ATAACGC3950.031.1652323
TAACGCA4150.030.8041884
GTACTAG10950.029.4028111
GGGGAAT45600.028.9689969
GGGGGGA48900.028.2719427
GGGGGAA50400.026.4911168
GTATAAC5950.026.259781
CATGGGG35050.025.5310044
TACGTGG3000.025.2518272
GTACGTG3400.023.6735881
ATGGGGG30850.023.635225
Produced by FastQC (version 0.11.5)