##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931069_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1742779 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.279642456100287 33.0 33.0 33.0 27.0 33.0 2 30.161314773703378 33.0 33.0 33.0 27.0 33.0 3 30.203662656022363 33.0 33.0 33.0 27.0 33.0 4 33.69325657470052 37.0 37.0 37.0 27.0 37.0 5 33.63882913438824 37.0 37.0 37.0 27.0 37.0 6 33.7344700618954 37.0 37.0 37.0 27.0 37.0 7 33.69693173948045 37.0 37.0 37.0 27.0 37.0 8 33.68568590739273 37.0 37.0 37.0 27.0 37.0 9 33.729802803453566 37.0 37.0 37.0 27.0 37.0 10-11 33.590300032304725 37.0 37.0 37.0 27.0 37.0 12-13 33.45871851795322 37.0 37.0 37.0 27.0 37.0 14-15 34.89565114107985 40.0 37.0 40.0 27.0 40.0 16-17 34.82092594643383 38.5 37.0 40.0 27.0 40.0 18-19 34.742315864489996 38.5 37.0 40.0 22.0 40.0 20-21 34.63575473424915 37.0 37.0 40.0 22.0 40.0 22-23 34.50341724337969 37.0 37.0 40.0 22.0 40.0 24-25 34.35961673855377 37.0 37.0 40.0 22.0 40.0 26-27 34.16172302971289 37.0 33.0 40.0 15.0 40.0 28-29 34.00028661121118 37.0 33.0 40.0 15.0 40.0 30-31 33.824762061053065 37.0 33.0 40.0 15.0 40.0 32-33 33.632615495137365 37.0 33.0 40.0 15.0 40.0 34-35 33.46991328217749 37.0 33.0 40.0 15.0 40.0 36-37 33.20681681383583 37.0 33.0 40.0 6.0 40.0 38-39 32.98173463187243 37.0 33.0 40.0 6.0 40.0 40-41 32.72714124969374 37.0 33.0 40.0 6.0 40.0 42-43 32.48876736522531 37.0 33.0 40.0 6.0 40.0 44-45 32.17539372462028 37.0 33.0 40.0 2.0 40.0 46-47 31.968889342825452 37.0 33.0 40.0 2.0 40.0 48-49 31.898441225192638 37.0 33.0 40.0 2.0 40.0 50-51 31.105756094146187 37.0 33.0 38.5 2.0 40.0 52-53 31.242823387245313 37.0 33.0 38.5 2.0 40.0 54-55 31.66478824911248 37.0 33.0 40.0 2.0 40.0 56-57 31.57617259560736 37.0 33.0 40.0 2.0 40.0 58-59 31.386215349163606 37.0 33.0 40.0 2.0 40.0 60-61 31.369310451870263 37.0 33.0 40.0 2.0 40.0 62-63 31.15756329402638 37.0 33.0 40.0 2.0 40.0 64-65 30.90584893437435 37.0 33.0 40.0 2.0 40.0 66-67 30.620128254930776 37.0 33.0 37.0 2.0 40.0 68-69 30.34286303656402 37.0 27.0 37.0 2.0 40.0 70-71 30.081205649138532 37.0 27.0 37.0 2.0 40.0 72-73 29.483118341453505 37.0 27.0 37.0 2.0 40.0 74-75 29.309779094193814 37.0 27.0 37.0 2.0 40.0 76-77 28.969598841849713 35.0 27.0 37.0 2.0 37.0 78-79 28.71000941599595 33.0 27.0 37.0 2.0 37.0 80-81 28.46220260859237 33.0 27.0 37.0 2.0 37.0 82-83 28.182080458853363 33.0 27.0 37.0 2.0 37.0 84-85 27.786120041611703 33.0 27.0 37.0 2.0 37.0 86-87 27.5932596732001 33.0 27.0 37.0 2.0 37.0 88-89 27.388299090131333 33.0 27.0 37.0 2.0 37.0 90-91 27.078109157845027 33.0 24.5 37.0 2.0 37.0 92-93 26.77438734343253 33.0 22.0 37.0 2.0 37.0 94-95 26.498768633314953 33.0 22.0 37.0 2.0 37.0 96-97 26.164659718759523 33.0 18.5 37.0 2.0 37.0 98-99 25.797825197572383 33.0 15.0 37.0 2.0 37.0 100-101 25.088614792810795 33.0 2.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.24657841531201186 1101 2 -0.24550668139389842 1101 3 -0.23314467657714033 1101 4 -0.2649380899160434 1101 5 -0.331669558960094 1101 6 -0.42323282586418287 1101 7 -0.3687796153294869 1101 8 -0.39994687087899905 1101 9 -0.45418169380504736 1101 10-11 -0.43986600271911414 1101 12-13 -0.409972248441143 1101 14-15 -0.39292492883176067 1101 16-17 -0.24446984700064434 1101 18-19 -0.2574375817737149 1101 20-21 -0.2598665286637001 1101 22-23 -0.34130137689765405 1101 24-25 -0.2857004038690931 1101 26-27 -0.21090105700969985 1101 28-29 -0.3550161472272819 1101 30-31 -0.35414918065648493 1101 32-33 -0.42316674462556847 1101 34-35 -0.5195976295700575 1101 36-37 -0.4007228707137429 1101 38-39 -0.5636310642450368 1101 40-41 -0.627871474298999 1101 42-43 -0.4948064488064645 1101 44-45 -0.48340953082146143 1101 46-47 -0.5160927582615997 1101 48-49 -0.48370822004179814 1101 50-51 -0.3087256707002233 1101 52-53 -0.1586280335494159 1101 54-55 -0.012120714556843382 1101 56-57 -0.082410315830856 1101 58-59 -0.2819223530737993 1101 60-61 -0.3253704863665945 1101 62-63 -0.15638423221532705 1101 64-65 -0.060955936209534656 1101 66-67 -0.022458158921640603 1101 68-69 0.13270640104333253 1101 70-71 -0.10269308325191773 1101 72-73 -0.19523976220486716 1101 74-75 -0.12054042856687275 1101 76-77 -0.1940025106429033 1101 78-79 -0.16557865760561086 1101 80-81 -0.034332141571074004 1101 82-83 -0.17962135207650576 1101 84-85 -0.14488496402102058 1101 86-87 -0.11892681385157289 1101 88-89 -0.16582283700894251 1101 90-91 -0.07194321186045016 1101 92-93 -0.1024402523081065 1101 94-95 -0.11154973203558427 1101 96-97 -0.11212393418726663 1101 98-99 -0.2436026304006056 1101 100-101 -0.1083930242383957 1102 1 0.28427851060355636 1102 2 0.27741396597582835 1102 3 0.24953526161683115 1102 4 0.320238540030509 1102 5 0.23730368915299493 1102 6 0.33206896285481236 1102 7 0.28894918493673316 1102 8 0.2986905373923179 1102 9 0.26202848419194424 1102 10-11 0.33007976077126955 1102 12-13 0.3560748997480303 1102 14-15 0.45434027934176413 1102 16-17 0.45379240523232056 1102 18-19 0.37552720941220485 1102 20-21 0.47500302405499895 1102 22-23 0.440140839307837 1102 24-25 0.2552580036101162 1102 26-27 0.32505318684290074 1102 28-29 0.45398060623758596 1102 30-31 0.4331976908428956 1102 32-33 0.5127712118696124 1102 34-35 0.5547357995865809 1102 36-37 0.5851491161251303 1102 38-39 0.4904765526138277 1102 40-41 0.5639908619876763 1102 42-43 0.752240930608508 1102 44-45 0.6704599078466771 1102 46-47 0.6059095086885762 1102 48-49 0.5482839385915952 1102 50-51 0.498017803029871 1102 52-53 0.6499193528960632 1102 54-55 0.8930276917818674 1102 56-57 0.7044030177732736 1102 58-59 0.6527846775964292 1102 60-61 0.7631997336259957 1102 62-63 0.6733531234052599 1102 64-65 0.6260926462044942 1102 66-67 0.7395630435462266 1102 68-69 0.7705136540340369 1102 70-71 0.6982947430225224 1102 72-73 0.4716037088692566 1102 74-75 0.6282321608651706 1102 76-77 0.761026806600448 1102 78-79 0.46471024183772514 1102 80-81 0.6317243025199311 1102 82-83 0.623369780101843 1102 84-85 0.4435643701893035 1102 86-87 0.5941971637945187 1102 88-89 0.6147069747138936 1102 90-91 0.6539763012530173 1102 92-93 0.7058665343251675 1102 94-95 0.6477080878488977 1102 96-97 0.7088002815588936 1102 98-99 0.6530863099759507 1102 100-101 0.4125684532319269 1103 1 0.17068485901751274 1103 2 0.1386467774027409 1103 3 0.1540926556317288 1103 4 0.23307958372657822 1103 5 0.14441113356313195 1103 6 0.061523504593822054 1103 7 0.05229553126335418 1103 8 0.028881119721944515 1103 9 -0.06260081336186119 1103 10-11 0.0547710214843633 1103 12-13 -0.05886825689907482 1103 14-15 0.18440642446692834 1103 16-17 0.09500564243153065 1103 18-19 0.09904160800098794 1103 20-21 0.031069465493978043 1103 22-23 -0.004688229223127394 1103 24-25 -0.0816785348752731 1103 26-27 0.16517528127067038 1103 28-29 0.16135466009517785 1103 30-31 -0.013446482248156144 1103 32-33 0.09152080407319829 1103 34-35 0.10587675373165695 1103 36-37 0.02140989855416109 1103 38-39 0.11193200810873094 1103 40-41 0.22685799735565126 1103 42-43 0.24301962287201206 1103 44-45 0.16697073972216714 1103 46-47 0.1494482984291139 1103 48-49 0.07369941653628587 1103 50-51 0.2541767148137488 1103 52-53 0.48867829431132037 1103 54-55 0.41471005359270663 1103 56-57 0.455686253636884 1103 58-59 0.548499366806297 1103 60-61 0.44790523345850275 1103 62-63 0.4794712008103055 1103 64-65 0.5252851910657483 1103 66-67 0.6070786565837416 1103 68-69 0.45343725688886494 1103 70-71 0.2657900665920927 1103 72-73 0.3342033224935612 1103 74-75 0.5783923235896218 1103 76-77 0.4778533440567756 1103 78-79 0.6275272009135797 1103 80-81 0.6062243459452326 1103 82-83 0.6423546644482272 1103 84-85 0.6459578340612815 1103 86-87 0.6090296406641578 1103 88-89 0.6347982919163719 1103 90-91 0.695084551403184 1103 92-93 0.6402301055342647 1103 94-95 0.5160918419697396 1103 96-97 0.6440079092311031 1103 98-99 0.6645885407226686 1103 100-101 0.5218301574450592 1104 1 0.35733862668755023 1104 2 0.4062956709437273 1104 3 0.2788629583352318 1104 4 0.5199923886239617 1104 5 0.5612274074447825 1104 6 0.5199538447255634 1104 7 0.4888028636028352 1104 8 0.551511457739096 1104 9 0.4734307363151373 1104 10-11 0.41599579178706136 1104 12-13 0.4457558219328277 1104 14-15 0.4812091649750627 1104 16-17 0.40753035669760607 1104 18-19 0.5306409778532881 1104 20-21 0.6314744094044826 1104 22-23 0.6272357301344584 1104 24-25 0.5587365590653661 1104 26-27 0.5104734912705666 1104 28-29 0.34130200681386214 1104 30-31 0.4854327852580056 1104 32-33 0.511064369905661 1104 34-35 0.3897334479937129 1104 36-37 0.4355586176415045 1104 38-39 0.5034535610633455 1104 40-41 0.595539867921616 1104 42-43 0.5803870645357776 1104 44-45 0.5274834219733506 1104 46-47 0.48052113546826547 1104 48-49 0.49766828977720934 1104 50-51 0.5482526920184725 1104 52-53 0.6426130169300279 1104 54-55 0.9272728304717859 1104 56-57 0.865330896852754 1104 58-59 0.7556265910902482 1104 60-61 0.9103802548687199 1104 62-63 0.9678060672211082 1104 64-65 0.9707853443734962 1104 66-67 0.9010288157066064 1104 68-69 0.9089002991489359 1104 70-71 0.9828400026257604 1104 72-73 0.8157561713744137 1104 74-75 0.9313247307327082 1104 76-77 0.6770297114032822 1104 78-79 0.7848764571066802 1104 80-81 0.713142754505089 1104 82-83 0.8576642932320233 1104 84-85 0.9560460124480592 1104 86-87 1.0601720477015135 1104 88-89 0.8427258883680899 1104 90-91 0.9105860791941787 1104 92-93 1.0039966125494146 1104 94-95 0.9099119538315321 1104 96-97 0.9024171034667319 1104 98-99 0.8333824845380242 1104 100-101 0.853817554490977 1105 1 -0.40088944481485456 1105 2 -0.5527694527231652 1105 3 -0.4487471568855028 1105 4 -0.5976436953838018 1105 5 -0.5311609645896098 1105 6 -0.5677752730920531 1105 7 -0.6435320581126192 1105 8 -0.5947104619341488 1105 9 -0.6908886545529285 1105 10-11 -0.5172719892683233 1105 12-13 -0.622065573322196 1105 14-15 -0.5374757554797753 1105 16-17 -0.697733995469406 1105 18-19 -0.694515969427556 1105 20-21 -0.674748014517057 1105 22-23 -0.6625229870578835 1105 24-25 -0.7202135959909839 1105 26-27 -0.7726291484692496 1105 28-29 -0.7756180446194634 1105 30-31 -0.8325991336588672 1105 32-33 -0.6454013538167018 1105 34-35 -0.6836876245424008 1105 36-37 -0.6314988414513536 1105 38-39 -0.8001165168430191 1105 40-41 -0.8474825963397841 1105 42-43 -0.7951064684798865 1105 44-45 -0.5835898705158655 1105 46-47 -0.7904745027840114 1105 48-49 -0.8932981020741408 1105 50-51 -0.742983472669021 1105 52-53 -0.76807200437446 1105 54-55 -0.6357632484934967 1105 56-57 -0.7403343327062828 1105 58-59 -0.654864294621877 1105 60-61 -0.7563544853173454 1105 62-63 -0.744570707102568 1105 64-65 -0.7232443558508947 1105 66-67 -0.5050801523492616 1105 68-69 -0.5309529645981321 1105 70-71 -0.5666242335641378 1105 72-73 -0.6501868989934358 1105 74-75 -0.6093202028325813 1105 76-77 -0.6348201234553983 1105 78-79 -0.47771588336176407 1105 80-81 -0.5903063038112215 1105 82-83 -0.5084678702452727 1105 84-85 -0.42488583839529426 1105 86-87 -0.26630350886254206 1105 88-89 -0.2941144918351206 1105 90-91 -0.2970255487714297 1105 92-93 -0.4028082181492181 1105 94-95 -0.3690382012248179 1105 96-97 -0.44751860487605555 1105 98-99 -0.25125559125049435 1105 100-101 -0.08350942531387773 1106 1 -0.08309843657798766 1106 2 -0.15202412586318914 1106 3 -0.08502995877339714 1106 4 -0.08404585184965185 1106 5 -0.21438088220443063 1106 6 -0.15781943511073848 1106 7 -0.09054406945583793 1106 8 -0.08878793435557242 1106 9 -0.11205639928755318 1106 10-11 -0.031005931664687125 1106 12-13 -0.2125229809770559 1106 14-15 -0.2431234458408369 1106 16-17 -0.24859438773106746 1106 18-19 -0.2511464585421237 1106 20-21 -0.40617711855762906 1106 22-23 -0.38568503378680674 1106 24-25 -0.08551976894046476 1106 26-27 -0.09320383210125271 1106 28-29 -0.10967492899482068 1106 30-31 -0.08317050142769489 1106 32-33 0.07571294118294247 1106 34-35 -0.07258393700559651 1106 36-37 -0.03658910386645431 1106 38-39 -9.712176977174636E-4 1106 40-41 0.06432831037558628 1106 42-43 0.04844615723835233 1106 44-45 -0.08519633199494336 1106 46-47 0.008960480727406406 1106 48-49 -0.010502647941105892 1106 50-51 -0.05867476415648909 1106 52-53 -0.024247345900121786 1106 54-55 0.21273793185481793 1106 56-57 0.2816003497379569 1106 58-59 0.311955792580374 1106 60-61 0.40003646979972274 1106 62-63 0.2770988712549105 1106 64-65 0.33642221018509844 1106 66-67 0.2524177401804799 1106 68-69 0.37722533975851746 1106 70-71 0.281759984246829 1106 72-73 0.022923100231214022 1106 74-75 0.11580051273399405 1106 76-77 0.1719970273107947 1106 78-79 0.2374610369106982 1106 80-81 0.309922342277293 1106 82-83 0.2550973920460997 1106 84-85 0.2612078273162055 1106 86-87 0.3119104451475927 1106 88-89 0.3362121289373583 1106 90-91 0.40285138486346384 1106 92-93 0.299478572672605 1106 94-95 0.3293884959279936 1106 96-97 0.30606129010511296 1106 98-99 0.6321928074845253 1106 100-101 0.644009764625693 1107 1 -0.13298805632999944 1107 2 -0.10035703531885432 1107 3 -0.1048674337513198 1107 4 -0.020782031326028516 1107 5 -0.043617617726972924 1107 6 -0.045420528371018065 1107 7 -0.12051331564492784 1107 8 -0.1670248034543249 1107 9 -0.17205245143795622 1107 10-11 -0.10631694995023366 1107 12-13 -0.26335764459805944 1107 14-15 -0.16685096038171565 1107 16-17 -0.23326988043803 1107 18-19 -0.12445958964670467 1107 20-21 -0.23264926447665601 1107 22-23 -0.1814995624364073 1107 24-25 -0.1667899441880749 1107 26-27 -0.15430087130790326 1107 28-29 -0.21850685039319728 1107 30-31 -0.19181175608057544 1107 32-33 -0.05303778965410544 1107 34-35 0.10198609305999895 1107 36-37 -0.12326536174299463 1107 38-39 -0.24543367042597453 1107 40-41 -0.22359114473358943 1107 42-43 -0.11057835477834743 1107 44-45 -0.2268263746924042 1107 46-47 -0.2567846977814021 1107 48-49 -0.15169942822262783 1107 50-51 -0.24408151623059737 1107 52-53 -0.3056612954483562 1107 54-55 -0.08927694171545397 1107 56-57 -0.05781899103372723 1107 58-59 -0.023719660397826914 1107 60-61 -0.06839371567100017 1107 62-63 -0.1595711457658915 1107 64-65 -0.16490213381337426 1107 66-67 -0.16173467496812677 1107 68-69 -0.17864721164407982 1107 70-71 -0.21305697724161732 1107 72-73 -0.17608511986734499 1107 74-75 -0.1421830120325076 1107 76-77 -0.2091781743126404 1107 78-79 0.0112358036450253 1107 80-81 -0.033555275261168305 1107 82-83 0.006523795151576195 1107 84-85 0.3023496129892642 1107 86-87 0.27832009491827847 1107 88-89 -0.006762309831401581 1107 90-91 3.041459650674483E-4 1107 92-93 0.14929621142684724 1107 94-95 0.16423418099407883 1107 96-97 0.15587110823813077 1107 98-99 0.035217417102575865 1107 100-101 0.05234645300553353 1108 1 -0.11713677339317385 1108 2 -0.1024525801239804 1108 3 -0.24645870959975724 1108 4 0.0010496770250938425 1108 5 -0.12409623875797848 1108 6 -0.1787891575510443 1108 7 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-0.8995333135165993 2316 84-85 -0.9467121330157049 2316 86-87 -0.7923111341348701 2316 88-89 -0.7035958057282024 2316 90-91 -0.8139017009417024 2316 92-93 -0.6558289991111792 2316 94-95 -0.9001342293796739 2316 96-97 -0.9413094545492591 2316 98-99 -0.9040270844631628 2316 100-101 -0.9733472835340891 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84031.0 3 11537.0 4 8696.0 5 7323.0 6 7181.0 7 7683.0 8 7925.0 9 8225.0 10 8189.0 11 8317.0 12 8773.0 13 9612.0 14 9938.0 15 10809.0 16 12859.0 17 15816.0 18 13875.0 19 11045.0 20 10900.0 21 11195.0 22 11986.0 23 13395.0 24 15173.0 25 17152.0 26 19454.0 27 22905.0 28 27157.0 29 32165.0 30 38992.0 31 48118.0 32 61181.0 33 79351.0 34 106139.0 35 150322.0 36 225320.0 37 347898.0 38 251757.0 39 385.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95276258326539 20.240410243227597 12.06830960579445 23.73851756771257 2 15.49022443180514 20.578615399446377 41.22683495609142 22.704325212657054 3 18.53129719482022 28.338007867907294 28.928100914862263 24.202594022410224 4 12.2009131476856 16.89466668732352 39.6724626354817 31.231957529509174 5 12.76445333972937 37.412049719236194 34.80271938653137 15.020777554503065 6 28.386444867651033 38.13575903772079 19.805265039342338 13.67253105528584 7 26.668613748501674 31.63900873260465 24.131631147724413 17.560746371169262 8 25.83953559229254 34.66532474857684 21.010007579848047 18.48513207928257 9 26.897214161979228 15.252478943113267 21.835987236476914 36.01431965843059 10-11 25.221019194349715 26.03529364173359 28.65410033460936 20.089586829307336 12-13 25.37727851088277 24.39238514382932 28.522573879912954 21.707762465374955 14-15 22.367853277803345 25.692221708339027 26.724486177370803 25.215438836486832 16-17 21.47754545780583 27.645604848832622 27.7674568733637 23.109392819997847 18-19 21.278327574596986 27.961923768924855 29.30873071865759 21.451017937820566 20-21 22.397912299239405 27.08970187548217 28.5664529370772 21.945932888201224 22-23 22.369847238232733 27.246053573057743 28.099259860257668 22.28483932845186 24-25 22.041469986002237 27.19921091521384 28.93138743678557 21.827931661998353 26-27 22.156452424547233 27.156627432393893 28.392211519647642 22.294708623411232 28-29 21.62465808917826 27.667219997486775 28.647321318423046 22.06080059491192 30-31 22.46920005347781 27.269866116128323 28.21640035827836 22.044533472115514 32-33 22.61723947786839 27.567293386023128 27.761752924495873 22.0537142116126 34-35 22.495594679531944 27.15745943691082 28.549603822400886 21.79734206115635 36-37 22.517398935837534 27.019977862941886 28.38633010840732 22.07629309281326 38-39 22.5404942336349 27.158033233129387 28.549804651077388 21.751667882158323 40-41 22.293038401400068 26.643313106970208 28.681355883576483 22.38229260805325 42-43 22.342190260497745 26.96124981997144 28.02874030499564 22.667819614535176 44-45 22.268566056173203 27.659264372446057 27.94713933934502 22.125030232035716 46-47 22.541087973166753 27.116718199763035 27.744365009832094 22.597828817238117 48-49 22.889522074412696 26.834890304357884 27.847993552507315 22.427594068722104 50-51 22.326912661189024 27.359179275614505 27.501555711766944 22.812352351429528 52-53 22.054145559917256 27.68359093284275 27.4022827678236 22.859980739416397 54-55 22.186087430139775 27.530930799874024 27.429368268387627 22.853613501598574 56-57 22.865086472847157 27.06970204164326 27.27169648859461 22.793514996914972 58-59 22.51615092208966 27.130815202486186 27.791125964386097 22.561907911038055 60-61 22.485887897253534 27.85818271792482 27.272005385903437 22.383923998918213 62-63 22.557569846907143 27.303146293363483 27.113732902377592 23.02555095735178 64-65 21.844643855276946 27.549984479862516 27.482294241669397 23.12307742319114 66-67 22.40463470569843 26.942434071909734 27.228522044680464 23.42440917771137 68-69 22.026850165516493 27.28300171081159 27.227930199807492 23.462217923864426 70-71 22.236024916179552 26.887286702598413 27.539212322216812 23.337476059005223 72-73 22.804556138130536 26.744472970529742 27.72321822455252 22.7277526667872 74-75 22.706208838284038 27.369948449593394 27.20072926372634 22.723113448396226 76-77 22.66714713590956 27.032949391054323 27.202759464219046 23.09714400881707 78-79 22.29942845823651 27.26044897022576 27.36159381003945 23.07852876149828 80-81 22.10207581339119 27.219363169355788 27.823548523465387 22.855012493787637 82-83 22.652543796855326 26.532314494911947 28.444362859622185 22.37077884861054 84-85 22.910335537954264 27.022703021190885 27.271819719950134 22.795141720904716 86-87 22.149013056848894 27.540643742111993 27.63573128823204 22.67461191280708 88-89 22.758973783708765 26.771422531139844 27.39857708055588 23.07102660459551 90-91 22.668697167520346 26.64408146780588 27.75174768246173 22.93547368221204 92-93 22.902515130115546 26.891810760266743 27.510385780566743 22.69528832905097 94-95 21.94384128894952 27.002471452396513 27.90858443094219 23.14510282771177 96-97 22.14124177070401 27.16778899902626 27.47416174170735 23.21680748856238 98-99 21.95712842392735 27.58000705733336 27.15329125752715 23.309573261212137 100-101 22.28167501326769 26.999599742432963 27.004743037802648 23.713982206496702 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 109.0 1 66.0 2 66.0 3 113.0 4 83.5 5 65.5 6 113.0 7 201.5 8 300.5 9 355.0 10 364.5 11 365.5 12 412.0 13 553.0 14 688.0 15 832.5 16 1020.5 17 1223.0 18 1464.5 19 1666.0 20 1932.0 21 2248.5 22 2605.0 23 3166.0 24 3848.5 25 4777.5 26 6369.5 27 8534.5 28 11713.0 29 17007.0 30 23384.5 31 28693.5 32 34531.0 33 39631.0 34 43947.0 35 49089.5 36 52520.5 37 57443.0 38 58915.0 39 55378.5 40 52918.5 41 50661.5 42 49835.5 43 51551.0 44 54324.0 45 58983.5 46 64560.0 47 68358.5 48 72015.0 49 70925.5 50 66154.0 51 64111.5 52 61686.0 53 58779.5 54 56139.0 55 51114.0 56 45783.0 57 39381.5 58 33065.5 59 30117.0 60 26032.0 61 20405.0 62 16004.5 63 13265.5 64 10547.0 65 7601.5 66 5791.0 67 4647.5 68 3835.5 69 3179.5 70 2618.0 71 2135.0 72 1832.5 73 1527.0 74 1258.5 75 1054.0 76 871.0 77 716.0 78 581.5 79 477.5 80 395.5 81 328.0 82 279.5 83 224.0 84 187.5 85 170.0 86 131.0 87 107.5 88 92.5 89 68.5 90 55.0 91 43.5 92 34.0 93 27.0 94 18.5 95 11.5 96 5.0 97 4.0 98 4.5 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.5903697485452833E-4 2 0.003844434664406675 3 1.7213886557044812E-4 4 9.180739497090567E-4 5 0.0018935275212749291 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.868981092840802E-5 12-13 8.606943278522406E-5 14-15 0.003270638445838514 16-17 0.005250235399898668 18-19 0.003700985609764634 20-21 2.868981092840802E-5 22-23 0.0 24-25 2.868981092840802E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 2.2951848742726417E-4 42-43 0.0 44-45 4.8772678578293634E-4 46-47 0.7450456999998278 48-49 0.5389094084792162 50-51 0.4153710826214913 52-53 0.3483803741036586 54-55 0.3395726021486373 56-57 0.30726787504324987 58-59 0.3063784909044692 60-61 0.2816765636951099 62-63 0.2743606619083659 64-65 0.27051622724395924 66-67 0.2583517474103142 68-69 0.23700652807957864 70-71 0.2519252297623508 72-73 0.8229098468595273 74-75 0.5464835185643159 76-77 0.6391229180521455 78-79 0.3534871604489152 80-81 0.3050013799799057 82-83 0.2859226557125143 84-85 0.2776313003542044 86-87 0.2513801233547111 88-89 0.2554253866956166 90-91 0.2650938529784901 92-93 0.22165747923288034 94-95 0.19027082607720197 96-97 0.14795335495780015 98-99 0.15750706199696002 100-101 0.1520273081096341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1742779.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.03904209439824 #Duplication Level Percentage of deduplicated Percentage of total 1 90.03298742326854 54.05494321790047 2 6.284731084654789 7.546584682871241 3 1.3925849597103028 2.508284010482183 4 0.5715977103155114 1.3727271596277857 5 0.31419432483832566 0.9431963147394636 6 0.20828661969259193 0.7503197476454067 7 0.14398964933961964 0.6051500432501346 8 0.10192113906248834 0.4895398046785404 9 0.08387910629012979 0.45324190740542464 >10 0.6404487058821334 7.817184602232981 >50 0.11255358250793274 4.748091137546492 >100 0.10200315075201859 12.229948969214064 >500 0.007566203107688845 3.117598197180561 >1k 0.0032563405779926675 3.3631902052252363 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 3998 0.2294037281835505 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 3588 0.20587808322225595 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 3066 0.17592592061299797 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 2677 0.15360524771069656 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2648 0.15194123867684886 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2539 0.14568685989445593 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 2527 0.14499830443217412 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2219 0.1273253809002748 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 2112 0.12118576136159546 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1988 0.11407068825135028 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 1952 0.11200502186450492 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1881 0.10793106871267097 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 1875 0.10758679098153007 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 5.737962185681604E-5 6 0.0 0.0 0.0 0.0 5.737962185681604E-5 7 0.0 0.0 0.0 0.0 5.737962185681604E-5 8 0.0 0.0 0.0 0.0 5.737962185681604E-5 9 0.0 0.0 0.0 0.0 5.737962185681604E-5 10-11 0.0 0.0 0.0 0.0 5.737962185681604E-5 12-13 0.0 0.0 0.0 0.0 1.1475924371363208E-4 14-15 0.0 0.0 0.0 0.0 1.434490546420401E-4 16-17 0.0 0.0 0.0 0.0 1.7213886557044812E-4 18-19 0.0 0.0 0.0 0.0 1.7213886557044812E-4 20-21 0.0 0.0 0.0 0.0 1.7213886557044812E-4 22-23 0.0 0.0 0.0 1.7213886557044812E-4 1.7213886557044812E-4 24-25 0.0 2.868981092840802E-5 0.0 2.582082983556722E-4 1.7213886557044812E-4 26-27 0.0 5.737962185681604E-5 0.0 4.3034716392612027E-4 1.7213886557044812E-4 28-29 0.0 5.737962185681604E-5 0.0 0.0015779396010624411 1.7213886557044812E-4 30-31 0.0 5.737962185681604E-5 0.0 0.005737962185681604 1.7213886557044812E-4 32-33 0.0 5.737962185681604E-5 0.0 0.01755816428818571 1.7213886557044812E-4 34-35 0.0 5.737962185681604E-5 0.0 0.03689509685393271 1.7213886557044812E-4 36-37 0.0 5.737962185681604E-5 0.0 0.06851126849703836 1.7213886557044812E-4 38-39 0.0 5.737962185681604E-5 0.0 0.1126935773267867 2.0082867649885613E-4 40-41 0.0 5.737962185681604E-5 0.0 0.15997438573680312 2.2951848742726417E-4 42-43 0.0 5.737962185681604E-5 0.0 0.20877575412602517 2.2951848742726417E-4 44-45 0.0 5.737962185681604E-5 0.0 0.27111871327345577 2.2951848742726417E-4 46-47 0.0 5.737962185681604E-5 0.0 0.3466016058260973 2.2951848742726417E-4 48-49 0.0 5.737962185681604E-5 0.0 0.425756794177575 2.2951848742726417E-4 50-51 0.0 5.737962185681604E-5 0.0 0.507178477592397 2.2951848742726417E-4 52-53 0.0 5.737962185681604E-5 0.0 0.5905223783394222 3.155879202124882E-4 54-55 0.0 5.737962185681604E-5 0.0 0.6827027408523972 4.0165735299771226E-4 56-57 0.0 5.737962185681604E-5 0.0 0.796457841183535 4.3034716392612027E-4 58-59 0.0 5.737962185681604E-5 0.0 0.9244717775460916 4.5903697485452833E-4 60-61 0.0 5.737962185681604E-5 0.0 1.057477741010191 5.164165967113443E-4 62-63 0.0 5.737962185681604E-5 0.0 1.1977709164501062 5.164165967113443E-4 64-65 0.0 5.737962185681604E-5 0.0 1.343572535588276 5.451064076397523E-4 66-67 0.0 5.737962185681604E-5 0.0 1.5118095868724606 6.024860294965684E-4 68-69 0.0 5.737962185681604E-5 0.0 1.69852287639454 6.311758404249765E-4 70-71 0.0 5.737962185681604E-5 0.0 1.9032246773687311 6.311758404249765E-4 72-73 0.0 5.737962185681604E-5 0.0 2.115557968049879 6.311758404249765E-4 74-75 0.0 5.737962185681604E-5 0.0 2.330243823227156 6.311758404249765E-4 76-77 0.0 5.737962185681604E-5 0.0 2.5791279330310957 6.311758404249765E-4 78-79 0.0 5.737962185681604E-5 0.0 2.8474924244554245 6.311758404249765E-4 80-81 0.0 5.737962185681604E-5 0.0 3.1441450694551634 6.311758404249765E-4 82-83 0.0 1.1475924371363208E-4 0.0 3.4513842546874844 6.311758404249765E-4 84-85 0.0 1.1475924371363208E-4 0.0 3.7660541009502637 6.311758404249765E-4 86-87 0.0 1.1475924371363208E-4 0.0 4.101897027678207 6.598656513533845E-4 88-89 0.0 1.1475924371363208E-4 0.0 4.477159754621785 6.885554622817925E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3145 0.0 61.423363 2 GTACCTG 3415 0.0 56.84434 1 ACCTGGG 3575 0.0 54.565136 3 CCTGGGG 3200 0.0 48.234936 4 CTGGGGG 2685 0.0 44.261234 5 GTACCCG 355 0.0 42.679146 1 GTACACG 275 0.0 41.32117 1 TGGGGGG 4720 0.0 33.80411 6 TATCACG 155 3.8198777E-11 33.601223 2 ATAACGC 395 0.0 31.165232 3 TAACGCA 415 0.0 30.804188 4 GTACTAG 1095 0.0 29.402811 1 GGGGAAT 4560 0.0 28.968996 9 GGGGGGA 4890 0.0 28.271942 7 GGGGGAA 5040 0.0 26.491116 8 GTATAAC 595 0.0 26.25978 1 CATGGGG 3505 0.0 25.531004 4 TACGTGG 300 0.0 25.251827 2 GTACGTG 340 0.0 23.673588 1 ATGGGGG 3085 0.0 23.63522 5 >>END_MODULE