FastQCFastQC Report
Sat 18 Jun 2016
SRR1931069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742779
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA45910.2634298439446424No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA39180.22481335843500524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36580.20989465675223307No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC34880.20014012103657433No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC34720.1992220470868653No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC29430.1688682271246096No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC28170.16163839477065078No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27610.15842513594666907No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT25010.14350643426389692No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA24040.13794061094378576No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21860.12543185337899987No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC21530.12353832585772492No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21400.12279239077358632No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT18230.10460305064497563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG34250.064.208662
GTACCTG36600.060.913551
ACCTGGG36400.059.7636683
CTGGGGG22350.052.2794155
CCTGGGG30600.050.4469684
TGGGGGG39800.039.0245446
TAACGCA2800.039.016024
GTACACG2900.037.7009281
ATAACGC3200.035.623323
GTACCCG2550.035.418941
GGGGGGA42750.031.1096487
GGGGAAT44050.029.9767469
GTATAAC4650.027.601521
GGGGGAA48350.027.114298
TATTCGC1955.366019E-1026.7943789
CTGTGCG15250.026.474929
CATGGGG34300.025.7568054
TAGTACT17550.024.899114
ATGGGGG29300.024.8025865
GCTATTG18200.023.7495638
Produced by FastQC (version 0.11.5)