##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931069_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1742779 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.64190296073111 33.0 33.0 33.0 33.0 33.0 2 32.592621898703165 33.0 33.0 33.0 33.0 33.0 3 32.65128395510848 33.0 33.0 33.0 33.0 33.0 4 36.598933083311195 37.0 37.0 37.0 37.0 37.0 5 36.543830284849655 37.0 37.0 37.0 37.0 37.0 6 36.54502033820697 37.0 37.0 37.0 37.0 37.0 7 36.48780941243841 37.0 37.0 37.0 37.0 37.0 8 36.494195764351076 37.0 37.0 37.0 37.0 37.0 9 36.490894714705654 37.0 37.0 37.0 37.0 37.0 10-11 36.467198365369335 37.0 37.0 37.0 37.0 37.0 12-13 36.37459999231113 37.0 37.0 37.0 37.0 37.0 14-15 38.32260401347503 40.0 37.0 40.0 37.0 40.0 16-17 38.32378373850041 40.0 37.0 40.0 37.0 40.0 18-19 38.28510069262941 40.0 37.0 40.0 37.0 40.0 20-21 38.19979412191678 40.0 37.0 40.0 35.0 40.0 22-23 38.06111962560944 40.0 37.0 40.0 33.0 40.0 24-25 38.22021897211293 40.0 37.0 40.0 37.0 40.0 26-27 38.16482698035723 40.0 37.0 40.0 37.0 40.0 28-29 38.07848442057197 40.0 37.0 40.0 35.0 40.0 30-31 37.948705487041096 40.0 37.0 40.0 33.0 40.0 32-33 37.81796343655736 40.0 37.0 40.0 33.0 40.0 34-35 37.72510169103484 40.0 37.0 40.0 33.0 40.0 36-37 37.6572548785589 40.0 37.0 40.0 33.0 40.0 38-39 37.5086740774361 40.0 37.0 40.0 33.0 40.0 40-41 37.332386952103505 40.0 37.0 40.0 33.0 40.0 42-43 37.14519081306351 40.0 37.0 40.0 33.0 40.0 44-45 36.90802649102382 40.0 37.0 40.0 33.0 40.0 46-47 36.63853621141865 40.0 37.0 40.0 33.0 40.0 48-49 36.36143653326096 37.0 37.0 40.0 33.0 40.0 50-51 36.10785360622316 37.0 37.0 40.0 33.0 40.0 52-53 35.880672764590344 37.0 37.0 40.0 33.0 40.0 54-55 35.64013681597036 37.0 37.0 40.0 27.0 40.0 56-57 35.337485418403595 37.0 37.0 40.0 27.0 40.0 58-59 35.057179940772755 37.0 33.0 40.0 27.0 40.0 60-61 34.80243823227156 37.0 33.0 40.0 27.0 40.0 62-63 34.48400227452821 37.0 33.0 40.0 27.0 40.0 64-65 34.130237970505725 37.0 33.0 38.5 27.0 40.0 66-67 33.77625304183721 37.0 33.0 37.0 27.0 40.0 68-69 33.4675744887906 37.0 33.0 37.0 27.0 40.0 70-71 33.17556586348584 37.0 33.0 37.0 24.5 40.0 72-73 32.8322105097663 37.0 33.0 37.0 22.0 40.0 74-75 32.44986685058748 37.0 33.0 37.0 22.0 38.5 76-77 30.818029996918714 33.0 30.0 37.0 22.0 37.0 78-79 31.590534141161903 33.0 33.0 37.0 22.0 37.0 80-81 31.648801138870734 33.0 33.0 37.0 22.0 37.0 82-83 31.42449243420996 33.0 33.0 37.0 22.0 37.0 84-85 31.128775937740816 33.0 33.0 37.0 15.0 37.0 86-87 30.79148188037611 33.0 33.0 37.0 15.0 37.0 88-89 30.46394494081005 33.0 33.0 37.0 15.0 37.0 90-91 30.06387040468126 33.0 30.0 37.0 10.5 37.0 92-93 29.668882285131964 33.0 27.0 37.0 4.0 37.0 94-95 29.188703788604293 33.0 27.0 37.0 2.0 37.0 96-97 28.70388787103815 33.0 27.0 37.0 2.0 37.0 98-99 28.037271220275205 33.0 27.0 37.0 2.0 37.0 100-101 26.32280742423451 33.0 21.0 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.006124104885316228 1101 2 0.15513227316251488 1101 3 0.08177263728897799 1101 4 0.0708379323984758 1101 5 -0.053585748035771985 1101 6 -0.008765446925067977 1101 7 0.040068598236764785 1101 8 0.05898935643369185 1101 9 0.09450058308385678 1101 10-11 0.1221085964982862 1101 12-13 0.16126908232536152 1101 14-15 0.0543383164051221 1101 16-17 0.08491392623756866 1101 18-19 0.07182468123568242 1101 20-21 0.01840412326153995 1101 22-23 -0.018614811980476986 1101 24-25 -0.005174718495524644 1101 26-27 -0.03329594260706159 1101 28-29 -0.06169184406899575 1101 30-31 -0.07545511212443756 1101 32-33 -0.15509463503077114 1101 34-35 0.00795910842745684 1101 36-37 -0.08074560916698204 1101 38-39 -0.09959456093238117 1101 40-41 -0.24637907961189143 1101 42-43 -0.29399833448683665 1101 44-45 -0.2568395183264798 1101 46-47 -0.33702689984149714 1101 48-49 -0.2935685224928264 1101 50-51 -0.40654384737461413 1101 52-53 -0.7286624405083941 1101 54-55 -0.6568977074932221 1101 56-57 -0.5626150871024436 1101 58-59 -0.5056644118462401 1101 60-61 -0.8069701257027262 1101 62-63 -0.8104834022855982 1101 64-65 -0.7868159361116724 1101 66-67 -0.7116588275681224 1101 68-69 -0.7379893278590828 1101 70-71 -1.00167535454667 1101 72-73 -1.163231292825106 1101 74-75 -1.2317345848660288 1101 76-77 -1.1194165889351844 1101 78-79 -0.9271225998073298 1101 80-81 -1.2791964601719954 1101 82-83 -1.486824923518526 1101 84-85 -1.413821550791372 1101 86-87 -1.7534823718253953 1101 88-89 -1.9412584724000723 1101 90-91 -2.0615435889146454 1101 92-93 -2.1724436215889753 1101 94-95 -2.4903934147478317 1101 96-97 -2.719000660417091 1101 98-99 -2.764965193788303 1101 100-101 -4.1569346871122335 1102 1 0.04570132901456958 1102 2 0.17777326230896762 1102 3 0.12025571796647228 1102 4 0.13696147883970866 1102 5 0.14836498524684316 1102 6 0.15090025994624767 1102 7 0.15420391532075683 1102 8 0.16601799562302233 1102 9 0.12595978969816457 1102 10-11 0.14951158712270995 1102 12-13 0.1585707319964058 1102 14-15 0.22596820046413768 1102 16-17 0.29782835550825126 1102 18-19 0.17927222188377812 1102 20-21 0.01826780940689332 1102 22-23 0.09622902468036898 1102 24-25 0.22417177960545587 1102 26-27 0.05211718601852766 1102 28-29 0.15908367630621711 1102 30-31 0.22254826095994673 1102 32-33 0.20693406665201763 1102 34-35 0.20805886360933812 1102 36-37 0.11481048105482472 1102 38-39 0.22057094517869302 1102 40-41 0.22965350760163972 1102 42-43 0.22167170474342868 1102 44-45 0.005704480973598436 1102 46-47 0.057894061557860255 1102 48-49 -0.029130190255735045 1102 50-51 -0.02506522873000705 1102 52-53 0.051894126226024184 1102 54-55 0.018807921761215596 1102 56-57 0.26726463642650344 1102 58-59 0.009110213310194126 1102 60-61 -0.17162706786658788 1102 62-63 -0.25023228794913166 1102 64-65 -0.04736444119004801 1102 66-67 -0.13344709348402972 1102 68-69 -0.23096193853891123 1102 70-71 -0.24987261358361224 1102 72-73 -0.41152014383601454 1102 74-75 -0.4203638611761633 1102 76-77 -0.5061071619533202 1102 78-79 -0.2773232428650054 1102 80-81 -0.39108279394642764 1102 82-83 -0.7314055689822041 1102 84-85 -0.6383455068772186 1102 86-87 -0.5763981759521499 1102 88-89 -0.8820992359451374 1102 90-91 -0.9482667757060277 1102 92-93 -0.9658090036505413 1102 94-95 -1.1217626698414378 1102 96-97 -1.3449435304603803 1102 98-99 -1.2723966423710316 1102 100-101 -2.2410762691653616 1103 1 -0.003471072346137305 1103 2 0.18274199244371658 1103 3 0.14964917235056419 1103 4 0.07264532513895716 1103 5 0.07792002553748745 1103 6 0.11386760193106227 1103 7 0.08964509082538541 1103 8 0.09932950683076314 1103 9 0.06310878372736539 1103 10-11 0.16137928373532162 1103 12-13 0.15595499627329446 1103 14-15 0.19083275366888586 1103 16-17 0.17905724041222726 1103 18-19 0.14165642412451973 1103 20-21 0.14358173053633294 1103 22-23 0.17596407926122737 1103 24-25 0.14942114665433337 1103 26-27 0.20843366960558996 1103 28-29 0.15999068120853366 1103 30-31 0.19488798693534903 1103 32-33 0.264035234464167 1103 34-35 0.08007300930961492 1103 36-37 0.13095538726712874 1103 38-39 0.289414642562015 1103 40-41 0.23844676595152237 1103 42-43 0.24261893825987357 1103 44-45 0.2149923498423263 1103 46-47 0.11865645656168766 1103 48-49 0.1964472750058519 1103 50-51 -0.014301045625579434 1103 52-53 0.08111825843161569 1103 54-55 0.05424296663525041 1103 56-57 0.21942564722152724 1103 58-59 0.20073128341176272 1103 60-61 0.04567733034998156 1103 62-63 -0.07878573646533482 1103 64-65 -0.018117136660457334 1103 66-67 0.12945332245006824 1103 68-69 -0.15785216156979232 1103 70-71 -0.22242362736366061 1103 72-73 -0.19429766982003827 1103 74-75 -0.29388147104764784 1103 76-77 -0.38725485249368674 1103 78-79 -0.25372633620605 1103 80-81 -0.3424636090137696 1103 82-83 -0.543433136384472 1103 84-85 -0.5133782583115298 1103 86-87 -0.4599720270610277 1103 88-89 -0.47335038817526254 1103 90-91 -0.5506824266220853 1103 92-93 -0.6502369829727996 1103 94-95 -0.8384414153100614 1103 96-97 -0.7204616234839456 1103 98-99 -0.725282381283531 1103 100-101 -1.20030447386128 1104 1 0.13910407488216237 1104 2 0.24446263401020474 1104 3 0.16914145938496006 1104 4 0.17509296261371787 1104 5 0.11199980900351392 1104 6 0.03364641585775274 1104 7 0.16321060609801208 1104 8 0.07656700696292518 1104 9 0.10862215322018187 1104 10-11 0.11838280452702321 1104 12-13 0.16762012986249175 1104 14-15 0.2500793253904874 1104 16-17 0.2002803190432445 1104 18-19 0.1921528240357091 1104 20-21 0.15710188786179202 1104 22-23 0.23874095833841835 1104 24-25 0.3062623726933893 1104 26-27 0.1988218025286912 1104 28-29 0.27686352036227646 1104 30-31 0.2280558536316235 1104 32-33 0.3023827884126362 1104 34-35 0.28608002974711866 1104 36-37 0.3781133788299158 1104 38-39 0.15832383151116147 1104 40-41 0.14920632653979737 1104 42-43 0.19728055105737496 1104 44-45 0.2929990319454916 1104 46-47 0.14138353705353524 1104 48-49 0.15364533804176972 1104 50-51 0.4171434305376991 1104 52-53 0.31768268469058825 1104 54-55 0.34937412405587054 1104 56-57 0.5052494384692352 1104 58-59 0.3905320890704047 1104 60-61 0.46719169369990965 1104 62-63 0.21742439030431626 1104 64-65 0.22416038208009326 1104 66-67 0.46172195991424303 1104 68-69 0.3553391770711656 1104 70-71 0.5965130512504331 1104 72-73 0.41969351234128993 1104 74-75 0.3461820723701621 1104 76-77 0.31111532756362337 1104 78-79 0.3967431026875978 1104 80-81 0.28472895608916104 1104 82-83 0.3542217837369037 1104 84-85 0.3888859385144734 1104 86-87 0.4150081486613679 1104 88-89 0.4073011168838896 1104 90-91 0.4508205179439493 1104 92-93 0.38038438918439965 1104 94-95 0.13677559444876763 1104 96-97 0.2933598628610916 1104 98-99 0.3246282773054041 1104 100-101 -0.4671048883208435 1105 1 0.049842042257303376 1105 2 0.13476846059921854 1105 3 0.07362674794104151 1105 4 0.0507907161875778 1105 5 0.01806339471311702 1105 6 0.056372886371832465 1105 7 -0.010336528511075471 1105 8 0.09503762189371656 1105 9 -0.0449970888946325 1105 10-11 -0.04437845911920135 1105 12-13 0.04780317199244166 1105 14-15 -0.017842919522912837 1105 16-17 0.020349401059228 1105 18-19 0.07254512920677314 1105 20-21 -0.02408208712748916 1105 22-23 0.02801811841204227 1105 24-25 0.021243589444523536 1105 26-27 0.0884998399943413 1105 28-29 0.04584216544711239 1105 30-31 0.03611122009407097 1105 32-33 9.482020222932874E-4 1105 34-35 -0.008416100147236705 1105 36-37 -0.03675438205554826 1105 38-39 0.006885463258633706 1105 40-41 -0.09477168823467252 1105 42-43 0.029968446577122165 1105 44-45 -0.028151662656526355 1105 46-47 0.041131412907610354 1105 48-49 0.044324308366761045 1105 50-51 0.06604542988218043 1105 52-53 0.18928846337601613 1105 54-55 0.13691919124695318 1105 56-57 0.15021325865428992 1105 58-59 0.1218736416508932 1105 60-61 0.10803832658194068 1105 62-63 -0.04117430369261399 1105 64-65 -0.026962830990022724 1105 66-67 0.11234092469250356 1105 68-69 -0.01636240878971762 1105 70-71 -0.04479749006010536 1105 72-73 -0.10782583818691904 1105 74-75 0.1366442159296568 1105 76-77 -0.14041632662290127 1105 78-79 0.13522228983935136 1105 80-81 -0.08279997515658621 1105 82-83 -0.00862015963601337 1105 84-85 0.2747064765473972 1105 86-87 0.21707052417507455 1105 88-89 0.1515186495969978 1105 90-91 -0.08798138811459211 1105 92-93 0.02895610589770925 1105 94-95 -0.27704080874816484 1105 96-97 -0.2561522769165343 1105 98-99 -0.03756066452955409 1105 100-101 -1.1023493226803218 1106 1 0.1559641815004582 1106 2 0.2178613526093045 1106 3 0.15034097497374432 1106 4 0.18311444341576077 1106 5 0.10895532891715476 1106 6 0.13571472877099922 1106 7 0.10929816564591732 1106 8 0.06704480791364631 1106 9 0.07010394595162239 1106 10-11 0.14617017601542415 1106 12-13 0.16386679697325945 1106 14-15 0.3002963368329006 1106 16-17 0.20405449049680868 1106 18-19 0.21765786030676537 1106 20-21 0.14784409944837762 1106 22-23 0.1430432678320912 1106 24-25 0.3097688881609031 1106 26-27 0.2191877413828749 1106 28-29 0.23171625832757314 1106 30-31 0.39449466255394583 1106 32-33 0.3071447419767992 1106 34-35 0.2678508977758085 1106 36-37 0.3309702264333936 1106 38-39 0.4012230907172878 1106 40-41 0.41797871305075773 1106 42-43 0.48085275924487547 1106 44-45 0.39390790080430804 1106 46-47 0.4382358889929847 1106 48-49 0.5569509834138202 1106 50-51 0.34148560659624394 1106 52-53 0.4422665539337274 1106 54-55 0.530077955106357 1106 56-57 0.5308928291427861 1106 58-59 0.495841311159964 1106 60-61 0.43362623242462917 1106 62-63 0.3761881168545216 1106 64-65 0.6174331127689783 1106 66-67 0.6132637574624695 1106 68-69 0.6169029575966789 1106 70-71 0.6781382201367521 1106 72-73 0.6782971918851572 1106 74-75 0.6228502250655978 1106 76-77 0.6836108139647479 1106 78-79 0.9606399483780343 1106 80-81 0.8666202436903809 1106 82-83 0.9318978738756556 1106 84-85 1.093630982096105 1106 86-87 0.9772531111092526 1106 88-89 0.8573035935985054 1106 90-91 0.8506746479936744 1106 92-93 1.0004749856968438 1106 94-95 1.0394398561401452 1106 96-97 1.1843596527673732 1106 98-99 1.3347370306980757 1106 100-101 1.1130791742768373 1107 1 6.36946374733327E-4 1107 2 0.09679406930229817 1107 3 0.024336689898959207 1107 4 0.05753966685873024 1107 5 0.07870328906452073 1107 6 0.07616445203049693 1107 7 0.012537685935264165 1107 8 0.037172850252609635 1107 9 0.00642625815270037 1107 10-11 -0.002189667659742156 1107 12-13 0.06917346386516243 1107 14-15 0.08582799986923817 1107 16-17 0.08454570058778188 1107 18-19 0.0980037632553632 1107 20-21 0.14956948490333843 1107 22-23 0.11522484889267304 1107 24-25 0.09176172009231465 1107 26-27 0.20849272903099347 1107 28-29 0.16241163355628174 1107 30-31 0.1351096824636855 1107 32-33 0.1830710554579511 1107 34-35 0.18814062363801298 1107 36-37 0.28743720761416114 1107 38-39 0.28511298638081684 1107 40-41 0.246088184229734 1107 42-43 0.24161057118521256 1107 44-45 0.2593358123512033 1107 46-47 0.38182089930212015 1107 48-49 0.3998193745587457 1107 50-51 0.26119519877587294 1107 52-53 0.32369952323278284 1107 54-55 0.36199872783378595 1107 56-57 0.2698469055164594 1107 58-59 0.345481578224792 1107 60-61 0.4630226963151003 1107 62-63 0.3936611052108674 1107 64-65 0.5911645845441953 1107 66-67 0.4336151723849042 1107 68-69 0.5019224637901374 1107 70-71 0.6123963575713418 1107 72-73 0.6201414883217424 1107 74-75 0.6577319162718069 1107 76-77 0.4045572843150538 1107 78-79 0.7398444160173945 1107 80-81 0.8235001536719047 1107 82-83 0.9604628493173379 1107 84-85 0.8809978321094185 1107 86-87 1.036893950643556 1107 88-89 1.0829167850470007 1107 90-91 1.021888536666193 1107 92-93 0.973270798348544 1107 94-95 1.0873189505872816 1107 96-97 1.1738980077528787 1107 98-99 1.2404747702319732 1107 100-101 1.9859365195763452 1108 1 -0.011651735082878645 1108 2 0.06347251099066398 1108 3 0.06780054481900777 1108 4 0.03176171922456206 1108 5 0.062079179020955166 1108 6 0.04006685200197779 1108 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2316 72-73 0.0721243042632338 2316 74-75 0.05405874584418058 2316 76-77 -0.03613653778429082 2316 78-79 0.07863187589599718 2316 80-81 0.13635537929288333 2316 82-83 0.09049162182871129 2316 84-85 0.04321661564577184 2316 86-87 0.25272705174336707 2316 88-89 0.07749275718875381 2316 90-91 0.2568338456712276 2316 92-93 0.20668779686469563 2316 94-95 0.3710319246186877 2316 96-97 0.765039276627963 2316 98-99 0.8918196773507852 2316 100-101 1.3187563418076955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 15.0 5 44.0 6 61.0 7 148.0 8 383.0 9 766.0 10 1299.0 11 2119.0 12 3255.0 13 4598.0 14 5597.0 15 6660.0 16 8546.0 17 11158.0 18 11363.0 19 8860.0 20 8070.0 21 8268.0 22 8557.0 23 9977.0 24 11234.0 25 12983.0 26 15728.0 27 19580.0 28 23859.0 29 29595.0 30 37783.0 31 47834.0 32 62104.0 33 84636.0 34 120057.0 35 185535.0 36 298421.0 37 472401.0 38 221243.0 39 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.10003704012106 21.754266074407575 13.387103720952936 24.758593164518437 2 13.933099912897685 21.667590479108643 40.86865911779928 23.53065049019439 3 16.97581850596088 28.648956637645966 30.0441995227163 24.331025333676845 4 11.48923644363399 17.810577244733842 39.04901309919387 31.651173212438295 5 12.150364446668224 37.86492722255662 35.356691812329615 14.628016518445541 6 28.64235798113243 38.791206458191205 19.408484954202454 13.157950606473914 7 26.38687980518471 31.898422003019316 24.257751556565694 17.456946635230285 8 25.961237770250843 35.220300451175966 20.65924595143733 18.159215827135856 9 26.54014077516426 15.07982366094611 21.844594179755436 36.535441384134195 10-11 25.081407338509358 26.399216423883924 28.776769745332025 19.742606492274696 12-13 25.064623799116237 24.616976679200288 28.72627567809803 21.592123843585444 14-15 22.023270392435045 25.908803357867992 26.86948525983742 25.19844098985955 16-17 21.235308665068835 27.778565153699926 27.96438905908322 23.021737122148018 18-19 21.041193404327228 28.06936507727027 29.61052434072249 21.278917177680015 20-21 22.086736183991203 27.269292319909756 28.894943076546138 21.74902841955291 22-23 22.11181107874263 27.370854250596317 28.422364510933402 22.09497015972765 24-25 21.7178139052628 27.32411854859394 29.308764909377494 21.649302636765764 26-27 21.84416383259151 27.264185533564493 28.825599803532175 22.066050830311816 28-29 21.32582501854796 27.804558122401062 29.02355949893819 21.846057360112788 30-31 22.174699144297698 27.40731900028632 28.640493143422084 21.77748871199389 32-33 22.393229434139382 27.694504007679686 28.186677714156527 21.725588844024397 34-35 22.175531148814624 27.287108692496293 28.94735936111234 21.590000797576746 36-37 22.220545462161294 27.07971004929483 28.84932627716997 21.850418211373903 38-39 22.11531427007422 27.281983662276833 29.097879160064416 21.504822907584536 40-41 21.827111882823196 26.790347809820663 29.210223705970044 22.172316601386097 42-43 22.02751326759001 27.00399641587859 28.598373832585494 22.370116483945907 44-45 21.847864818201277 27.817325088264205 28.42463100599674 21.910179087537777 46-47 22.1515751566894 27.33441819071724 28.239352207021085 22.274654445572274 48-49 22.53228974832946 26.980972007566095 28.45026708848448 22.03647115561996 50-51 22.03345920509715 27.464124825924575 27.903107622940144 22.59930834603814 52-53 21.872595435221562 27.73524353919803 27.71478770400607 22.677373321574336 54-55 21.951062068110758 27.697745956314602 27.766515433109994 22.58467654246465 56-57 22.700841586913775 27.17866120718691 27.643206625739698 22.477290580159618 58-59 22.259649674456718 27.407118171609824 28.06328283735344 22.269949316580014 60-61 22.325951827512267 28.012444492388305 27.536738737384375 22.124864942715057 62-63 22.376245066069767 27.40792148631582 27.344775212462398 22.87105823515202 64-65 21.644568817962575 27.7522852868895 27.652473434669574 22.95067246047835 66-67 22.17662030189842 27.115599959489046 27.482149502062413 23.225630236550117 68-69 21.819231412586877 27.421830191640446 27.49264129727782 23.266297098494864 70-71 21.928151220120988 27.039533612679907 27.805015901523877 23.22729926567523 72-73 22.438243747486055 27.03845410117978 27.96570879038593 22.557593360948232 74-75 22.33910457901121 27.621647737118554 27.392473395923062 22.646774287947174 76-77 22.21431645388036 27.315363649404677 27.513728302969444 22.956591593745518 78-79 21.762536312448447 27.468808951690992 27.758103503927124 23.010551231933434 80-81 21.563854040366078 27.46724768046842 28.15660862075594 22.81228965840956 82-83 22.073768205923905 26.748520401078324 28.794997653069775 22.382713739927997 84-85 22.335141599024062 27.29647256214072 27.516442517463897 22.851943321371312 86-87 21.56424802190302 27.722305853285008 27.898920886736 22.814525238075976 88-89 21.985145563493706 27.029416810737334 27.74092412176185 23.244513504007106 90-91 21.854606923769452 26.855212278780037 28.1020140821068 23.188166715343712 92-93 22.126887992161848 27.084519791192978 27.80458554905086 22.984006667594315 94-95 20.98472041948267 27.190027302179697 28.260510306314618 23.56474197202301 96-97 21.12046074849826 27.48782871925907 27.77622689005953 23.61548364218314 98-99 20.84937332845989 27.9301334248347 27.446566661636385 23.77392658506902 100-101 19.57159714489904 28.215079911325354 27.5845626702923 24.6287602734833 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 115.0 1 75.5 2 68.5 3 95.0 4 74.5 5 79.0 6 125.0 7 230.5 8 356.0 9 419.5 10 459.5 11 508.5 12 596.5 13 765.0 14 910.0 15 1056.5 16 1230.0 17 1364.5 18 1532.0 19 1706.0 20 1906.0 21 2206.5 22 2545.0 23 3028.5 24 3786.0 25 4745.0 26 6283.0 27 8489.5 28 11826.5 29 17441.0 30 23599.0 31 28707.5 32 34953.5 33 40348.0 34 44666.0 35 49756.0 36 53056.0 37 58259.0 38 60004.0 39 55883.5 40 53347.5 41 51386.5 42 50945.5 43 52809.0 44 55809.5 45 61472.0 46 67007.5 47 70910.0 48 75259.0 49 73278.5 50 68119.5 51 66150.5 52 63458.0 53 60565.5 54 57461.0 55 51800.5 56 45641.5 57 38507.0 58 32211.0 59 29458.5 60 24724.5 61 18252.5 62 13388.0 63 10377.0 64 7766.0 65 4991.5 66 3389.0 67 2518.5 68 1939.0 69 1501.0 70 1132.5 71 874.0 72 673.5 73 546.0 74 450.0 75 344.5 76 251.5 77 194.5 78 158.5 79 104.5 80 87.0 81 77.5 82 53.5 83 43.5 84 35.0 85 27.0 86 22.0 87 18.5 88 15.0 89 10.0 90 7.0 91 5.5 92 2.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08170858152410604 2 5.737962185681604E-5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.7296754206930425E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.434490546420401E-4 40-41 0.002582082983556722 42-43 0.005307615021755484 44-45 0.0 46-47 0.0 48-49 2.5820829835567215E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0023238746852010496 68-69 0.0065699667026054365 70-71 0.0012336618699215448 72-73 0.0 74-75 5.737962185681604E-5 76-77 0.0016640090338476653 78-79 0.005250235399898668 80-81 0.004475610504831651 82-83 0.004418230882974835 84-85 0.0023525644961294575 86-87 3.155879202124882E-4 88-89 0.0 90-91 0.0 92-93 0.0012336618699215448 94-95 0.003500156933265779 96-97 0.002840291281912394 98-99 0.0 100-101 2.0082867649885613E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1742779.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.81293659894506 #Duplication Level Percentage of deduplicated Percentage of total 1 87.26339166510941 46.95899363083444 2 8.082571396423116 8.698938042243281 3 1.8045630003203188 2.913265029751182 4 0.714388515714042 1.5377337561253688 5 0.39213671254270716 1.0551014025089724 6 0.2368632379648744 0.7647783844334775 7 0.18633721139988554 0.7019146780160386 8 0.13758438154091038 0.5923055680692862 9 0.1111746899145211 0.5384372885799751 >10 0.7964403956919166 8.742652497028521 >50 0.12923567711025954 4.9876307533531925 >100 0.13074985879094927 14.420551127749148 >500 0.009958698127724722 3.691591048276164 >1k 0.004604559349378097 4.396106793030927 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 4591 0.2634298439446424 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 3918 0.22481335843500524 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3658 0.20989465675223307 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 3488 0.20014012103657433 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 3472 0.1992220470868653 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 2943 0.1688682271246096 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2817 0.16163839477065078 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2761 0.15842513594666907 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 2501 0.14350643426389692 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 2404 0.13794061094378576 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2186 0.12543185337899987 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 2153 0.12353832585772492 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2140 0.12279239077358632 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 1823 0.10460305064497563 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.737962185681604E-5 0.0 5 0.0 0.0 0.0 1.1475924371363208E-4 0.0 6 0.0 0.0 0.0 1.1475924371363208E-4 0.0 7 0.0 0.0 0.0 1.1475924371363208E-4 0.0 8 0.0 0.0 0.0 1.1475924371363208E-4 0.0 9 0.0 0.0 0.0 1.1475924371363208E-4 0.0 10-11 0.0 0.0 0.0 1.1475924371363208E-4 0.0 12-13 0.0 0.0 0.0 1.1475924371363208E-4 0.0 14-15 0.0 0.0 0.0 1.1475924371363208E-4 0.0 16-17 0.0 0.0 0.0 1.1475924371363208E-4 0.0 18-19 0.0 0.0 0.0 1.1475924371363208E-4 0.0 20-21 0.0 0.0 0.0 1.1475924371363208E-4 0.0 22-23 0.0 0.0 0.0 2.2951848742726417E-4 0.0 24-25 0.0 0.0 0.0 3.4427773114089624E-4 0.0 26-27 0.0 0.0 0.0 4.3034716392612027E-4 0.0 28-29 0.0 0.0 0.0 0.001520559979205625 2.868981092840802E-5 30-31 0.0 0.0 0.0 0.005737962185681604 8.606943278522406E-5 32-33 0.0 0.0 0.0 0.01738602542261526 1.1475924371363208E-4 34-35 0.0 0.0 0.0 0.03637868025722137 1.1475924371363208E-4 36-37 0.0 0.0 0.0 0.06810961114404064 1.1475924371363208E-4 38-39 0.0 0.0 0.0 0.11226323016286058 1.1475924371363208E-4 40-41 0.0 0.0 0.0 0.1592858302745213 1.1475924371363208E-4 42-43 0.0 0.0 0.0 0.20768554131074565 1.1475924371363208E-4 44-45 2.868981092840802E-5 0.0 0.0 0.2695694634833218 1.1475924371363208E-4 46-47 5.737962185681604E-5 0.0 0.0 0.3442490413299678 1.1475924371363208E-4 48-49 5.737962185681604E-5 0.0 0.0 0.42205580856781033 1.1475924371363208E-4 50-51 1.1475924371363208E-4 0.0 0.0 0.5022725199236392 1.1475924371363208E-4 52-53 1.1475924371363208E-4 0.0 0.0 0.5842966893679578 1.1475924371363208E-4 54-55 1.1475924371363208E-4 0.0 0.0 0.6743253160613021 1.1475924371363208E-4 56-57 1.1475924371363208E-4 0.0 0.0 0.786186888871165 1.434490546420401E-4 58-59 1.1475924371363208E-4 0.0 0.0 0.9113605339518092 1.7213886557044812E-4 60-61 1.1475924371363208E-4 0.0 0.0 1.04164096537771 1.7213886557044812E-4 62-63 1.1475924371363208E-4 0.0 0.0 1.1792086087794265 1.7213886557044812E-4 64-65 1.1475924371363208E-4 0.0 0.0 1.3233462188837484 1.7213886557044812E-4 66-67 1.1475924371363208E-4 0.0 0.0 1.4883700113439513 1.7213886557044812E-4 68-69 1.1475924371363208E-4 0.0 0.0 1.67095196809234 1.7213886557044812E-4 70-71 1.1475924371363208E-4 0.0 0.0 1.8715511261037687 1.7213886557044812E-4 72-73 1.1475924371363208E-4 0.0 0.0 2.0806998477718635 1.7213886557044812E-4 74-75 1.1475924371363208E-4 0.0 0.0 2.293033138453011 1.7213886557044812E-4 76-77 1.1475924371363208E-4 0.0 0.0 2.5369826007772645 1.7213886557044812E-4 78-79 1.1475924371363208E-4 0.0 0.0 2.7992074726629137 1.7213886557044812E-4 80-81 1.1475924371363208E-4 0.0 0.0 3.0888884936070493 1.7213886557044812E-4 82-83 1.1475924371363208E-4 0.0 0.0 3.387893703102918 1.7213886557044812E-4 84-85 1.1475924371363208E-4 0.0 0.0 3.6929524627046804 1.7213886557044812E-4 86-87 1.1475924371363208E-4 0.0 0.0 4.020303205397816 2.582082983556722E-4 88-89 1.1475924371363208E-4 0.0 0.0 4.383659660806103 3.4427773114089624E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3425 0.0 64.20866 2 GTACCTG 3660 0.0 60.91355 1 ACCTGGG 3640 0.0 59.763668 3 CTGGGGG 2235 0.0 52.279415 5 CCTGGGG 3060 0.0 50.446968 4 TGGGGGG 3980 0.0 39.024544 6 TAACGCA 280 0.0 39.01602 4 GTACACG 290 0.0 37.700928 1 ATAACGC 320 0.0 35.62332 3 GTACCCG 255 0.0 35.41894 1 GGGGGGA 4275 0.0 31.109648 7 GGGGAAT 4405 0.0 29.976746 9 GTATAAC 465 0.0 27.60152 1 GGGGGAA 4835 0.0 27.11429 8 TATTCGC 195 5.366019E-10 26.794378 9 CTGTGCG 1525 0.0 26.47492 9 CATGGGG 3430 0.0 25.756805 4 TAGTACT 1755 0.0 24.89911 4 ATGGGGG 2930 0.0 24.802586 5 GCTATTG 1820 0.0 23.749563 8 >>END_MODULE