##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931068_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3407477 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45656449038394 33.0 33.0 33.0 27.0 33.0 2 31.383461429086683 33.0 33.0 33.0 27.0 33.0 3 31.408230194950693 33.0 33.0 33.0 27.0 33.0 4 35.0679543838447 37.0 37.0 37.0 33.0 37.0 5 35.06791564550546 37.0 37.0 37.0 33.0 37.0 6 35.16048090713451 37.0 37.0 37.0 33.0 37.0 7 35.12786645368406 37.0 37.0 37.0 33.0 37.0 8 35.13183889429041 37.0 37.0 37.0 33.0 37.0 9 35.140650105635345 37.0 37.0 37.0 33.0 37.0 10-11 35.06095110840073 37.0 37.0 37.0 33.0 37.0 12-13 34.992780435495234 37.0 37.0 37.0 33.0 37.0 14-15 36.74185005504072 40.0 37.0 40.0 33.0 40.0 16-17 36.69472545229212 40.0 37.0 40.0 33.0 40.0 18-19 36.63924114528139 40.0 37.0 40.0 33.0 40.0 20-21 36.56355068574197 40.0 37.0 40.0 33.0 40.0 22-23 36.48955943062859 40.0 37.0 40.0 33.0 40.0 24-25 36.386723813542986 40.0 37.0 40.0 33.0 40.0 26-27 36.235962707892085 40.0 37.0 40.0 33.0 40.0 28-29 36.14404044400006 40.0 37.0 40.0 33.0 40.0 30-31 36.03868771528025 40.0 37.0 40.0 33.0 40.0 32-33 35.90350353061811 40.0 37.0 40.0 33.0 40.0 34-35 35.82653294504996 40.0 37.0 40.0 33.0 40.0 36-37 35.6588509621635 38.5 37.0 40.0 27.0 40.0 38-39 35.51973131440066 37.0 37.0 40.0 27.0 40.0 40-41 35.335432198075 37.0 37.0 40.0 27.0 40.0 42-43 35.20419345457064 37.0 37.0 40.0 27.0 40.0 44-45 35.01815331402091 37.0 37.0 40.0 27.0 40.0 46-47 34.94189938772881 37.0 37.0 40.0 27.0 40.0 48-49 34.98308807366858 37.0 37.0 40.0 27.0 40.0 50-51 34.19961558067743 37.0 35.0 38.5 24.5 40.0 52-53 34.323275549622196 37.0 35.0 38.5 27.0 40.0 54-55 34.87039178841119 37.0 37.0 40.0 27.0 40.0 56-57 34.86462006933576 37.0 37.0 40.0 27.0 40.0 58-59 34.74990528182582 37.0 37.0 40.0 27.0 40.0 60-61 34.79641784816155 37.0 37.0 40.0 27.0 40.0 62-63 34.65044283497732 37.0 37.0 40.0 27.0 40.0 64-65 34.459422323320155 37.0 33.0 40.0 27.0 40.0 66-67 34.229929358290605 37.0 33.0 40.0 24.5 40.0 68-69 34.003702005912295 37.0 33.0 40.0 22.0 40.0 70-71 33.75366099316298 37.0 33.0 40.0 22.0 40.0 72-73 33.13181849796785 37.0 33.0 38.5 22.0 40.0 74-75 32.92428092691455 37.0 33.0 37.0 22.0 40.0 76-77 32.538758295360466 37.0 33.0 37.0 15.0 40.0 78-79 32.25310647731445 37.0 33.0 37.0 15.0 37.0 80-81 31.956282757007603 37.0 33.0 37.0 15.0 37.0 82-83 31.665695762583283 37.0 33.0 37.0 15.0 37.0 84-85 31.270522882472868 37.0 33.0 37.0 10.5 37.0 86-87 31.083752729658926 37.0 33.0 37.0 6.0 37.0 88-89 30.855939746621914 37.0 33.0 37.0 2.0 37.0 90-91 30.541221554833676 35.0 33.0 37.0 2.0 37.0 92-93 30.25062120742121 33.0 33.0 37.0 2.0 37.0 94-95 29.97864416986527 33.0 33.0 37.0 2.0 37.0 96-97 29.659066664279756 33.0 33.0 37.0 2.0 37.0 98-99 29.30376287206047 33.0 30.0 37.0 2.0 37.0 100-101 28.557691071722566 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1285831826436734 1101 2 0.019470890381601436 1101 3 0.01529022202124608 1101 4 0.1030306083712631 1101 5 -0.021994865859788604 1101 6 -0.03990421629546148 1101 7 -0.004546779356402908 1101 8 0.085201526386399 1101 9 0.027524776663021555 1101 10-11 0.04253348462153639 1101 12-13 0.049455653420018564 1101 14-15 0.17457672921341327 1101 16-17 0.20751482870824844 1101 18-19 0.20562607574098735 1101 20-21 0.13379854753802078 1101 22-23 0.16469831042403626 1101 24-25 0.16152010306462472 1101 26-27 0.16562469484383513 1101 28-29 0.17107624655803733 1101 30-31 0.09681962831987079 1101 32-33 0.11483589679575346 1101 34-35 0.1412994357683175 1101 36-37 0.14234490448384207 1101 38-39 0.22579505239731645 1101 40-41 0.21293560817284884 1101 42-43 0.19278075268299943 1101 44-45 0.050043637241770966 1101 46-47 0.1069356883799486 1101 48-49 0.13912604171098764 1101 50-51 0.23909382765702958 1101 52-53 0.28628706493978484 1101 54-55 0.27892736728373535 1101 56-57 0.28684452375184577 1101 58-59 0.33809522333903175 1101 60-61 0.2404271936466813 1101 62-63 0.22416197541389948 1101 64-65 0.3124326129658428 1101 66-67 0.3456147366708322 1101 68-69 0.30855121123358487 1101 70-71 0.2385991642757901 1101 72-73 0.21362910534892876 1101 74-75 0.07705883175217565 1101 76-77 0.09120139024317808 1101 78-79 0.14015159778973896 1101 80-81 0.18367445414070005 1101 82-83 0.05705746809831069 1101 84-85 0.09683051261262321 1101 86-87 -0.012649075532607412 1101 88-89 0.01692659533334151 1101 90-91 -0.05314306303228378 1101 92-93 -0.2527368525914646 1101 94-95 -0.08989869748812396 1101 96-97 -0.20753277493541944 1101 98-99 -0.15961145641462338 1101 100-101 -0.03437453743567076 1102 1 0.14958149168847257 1102 2 0.0939514033209754 1102 3 0.1468214020028391 1102 4 0.21555810228568362 1102 5 0.1302311998869783 1102 6 0.16947545057951885 1102 7 0.19787346750936763 1102 8 0.1492145474260269 1102 9 0.264400558448628 1102 10-11 0.1995028312908218 1102 12-13 0.20963136019333461 1102 14-15 0.36314072780901085 1102 16-17 0.32877729449061377 1102 18-19 0.3226331583884985 1102 20-21 0.3360464225970645 1102 22-23 0.34826071488604526 1102 24-25 0.3195050986489747 1102 26-27 0.34143342424112433 1102 28-29 0.23669208090115745 1102 30-31 0.358046167416731 1102 32-33 0.4208688366812652 1102 34-35 0.21716087915359594 1102 36-37 0.337418933896096 1102 38-39 0.3579719542818154 1102 40-41 0.26982918459689387 1102 42-43 0.2315985168079564 1102 44-45 0.3268679911042156 1102 46-47 0.4633667215643342 1102 48-49 0.46993235545799195 1102 50-51 0.40570866053217713 1102 52-53 0.4410342822964708 1102 54-55 0.45877974366747765 1102 56-57 0.49357712202333204 1102 58-59 0.541322928207201 1102 60-61 0.5448575647277281 1102 62-63 0.5031244731767259 1102 64-65 0.5277597184202278 1102 66-67 0.5386212823985019 1102 68-69 0.5374593813149247 1102 70-71 0.5897488964515745 1102 72-73 0.5154204762940395 1102 74-75 0.49622232237661734 1102 76-77 0.5913440554193841 1102 78-79 0.5947796707756297 1102 80-81 0.5999865972332614 1102 82-83 0.4043789569212777 1102 84-85 0.5912959106227866 1102 86-87 0.49356132173917544 1102 88-89 0.4085921373045309 1102 90-91 0.48166430597247967 1102 92-93 0.43626541049142986 1102 94-95 0.477524229086864 1102 96-97 0.5598228655101956 1102 98-99 0.5692815547276879 1102 100-101 0.45391664915717556 1103 1 0.13807117187124973 1103 2 0.12492082345470479 1103 3 0.1314825226231875 1103 4 0.03931981270267926 1103 5 0.10613206279694509 1103 6 0.12182241740202926 1103 7 0.14215136031641862 1103 8 0.15414624557397616 1103 9 0.104288320341837 1103 10-11 0.15842321154377714 1103 12-13 0.10613111008586174 1103 14-15 0.26023914515403845 1103 16-17 0.1889396100937617 1103 18-19 0.19612906941699748 1103 20-21 0.23339337933911963 1103 22-23 0.15392889160595757 1103 24-25 0.189815751614276 1103 26-27 0.2434021609195156 1103 28-29 0.25687792654329655 1103 30-31 0.238577401056979 1103 32-33 0.15927916122511476 1103 34-35 0.1585200861213849 1103 36-37 0.22225441291183046 1103 38-39 0.1872038835411587 1103 40-41 0.1519930611036031 1103 42-43 0.2641871391918258 1103 44-45 0.2701539664259016 1103 46-47 0.13216652604815948 1103 48-49 0.18451509936429744 1103 50-51 0.22117986277962132 1103 52-53 0.39449122001780523 1103 54-55 0.4704935768170131 1103 56-57 0.5650489994377423 1103 58-59 0.35441917354862795 1103 60-61 0.4266803295924291 1103 62-63 0.43577018457846606 1103 64-65 0.36937056732731577 1103 66-67 0.4671832305351984 1103 68-69 0.44690653292350646 1103 70-71 0.45314995257039925 1103 72-73 0.38332184277909676 1103 74-75 0.47616153046894283 1103 76-77 0.40169107869248677 1103 78-79 0.5218913764100037 1103 80-81 0.6229292812929828 1103 82-83 0.586003349489431 1103 84-85 0.6251892808371728 1103 86-87 0.46433027732040344 1103 88-89 0.4113033336291494 1103 90-91 0.5693451746663953 1103 92-93 0.382887290784371 1103 94-95 0.3459045855364913 1103 96-97 0.423871367318295 1103 98-99 0.46829806002855534 1103 100-101 0.5279576443810505 1104 1 0.08028735666649212 1104 2 0.17213287220039675 1104 3 0.062334239445693385 1104 4 0.1207964190834403 1104 5 0.12696234276516094 1104 6 0.13025039926964865 1104 7 0.07281685078883271 1104 8 0.01689494650140233 1104 9 0.03473496014616728 1104 10-11 0.07198984081312432 1104 12-13 0.056579035030495106 1104 14-15 0.1898746393013937 1104 16-17 0.17451539757438894 1104 18-19 0.21308335233548092 1104 20-21 0.1376074396863629 1104 22-23 0.26101018327509706 1104 24-25 0.226836022917702 1104 26-27 0.27011250318530244 1104 28-29 0.2789847197195954 1104 30-31 0.19111600930951766 1104 32-33 0.24797124537562354 1104 34-35 0.3708605170122752 1104 36-37 0.2814530228183898 1104 38-39 0.25376170662454456 1104 40-41 0.33538198799360686 1104 42-43 0.3361799356630897 1104 44-45 0.2826859685019585 1104 46-47 0.2607441692476016 1104 48-49 0.3722984921386754 1104 50-51 0.28325476742042355 1104 52-53 0.32419578054047804 1104 54-55 0.38284576832830197 1104 56-57 0.40971906842082007 1104 58-59 0.3985693953444098 1104 60-61 0.3899682043927939 1104 62-63 0.264578637390521 1104 64-65 0.33498508155914664 1104 66-67 0.4335023903707551 1104 68-69 0.4307813439949868 1104 70-71 0.4016842976476269 1104 72-73 0.3544798632743067 1104 74-75 0.35094320040168014 1104 76-77 0.2912124948218988 1104 78-79 0.3202018433026623 1104 80-81 0.3483326279331962 1104 82-83 0.3071601800399151 1104 84-85 0.4614264098495937 1104 86-87 0.5360678802283125 1104 88-89 0.2749419148897836 1104 90-91 0.38184146848437095 1104 92-93 0.3431291054024861 1104 94-95 0.2958510975209201 1104 96-97 0.3024904602591256 1104 98-99 0.13751514184379943 1104 100-101 0.3073243895113542 1105 1 0.125089294449797 1105 2 0.04829350130027166 1105 3 0.06826728775898872 1105 4 0.10546100062734354 1105 5 0.10111807732376121 1105 6 0.12043448653715672 1105 7 0.05541210957311904 1105 8 0.13670156168034708 1105 9 0.05762647425937928 1105 10-11 0.11675367973327866 1105 12-13 0.12345005231306772 1105 14-15 0.190294442267664 1105 16-17 0.20165305681601353 1105 18-19 0.24664093831996325 1105 20-21 0.31935860295376983 1105 22-23 0.18659469674903306 1105 24-25 0.2708541615539204 1105 26-27 0.21403714756907277 1105 28-29 0.2431886913740371 1105 30-31 0.18228190249175924 1105 32-33 0.18061647985177132 1105 34-35 0.2483659644342353 1105 36-37 0.2997724944855378 1105 38-39 0.27754639760699007 1105 40-41 0.31547738897787525 1105 42-43 0.4361553771001283 1105 44-45 0.34584869643779825 1105 46-47 0.3765994136008288 1105 48-49 0.3968223547266305 1105 50-51 0.38724910231622545 1105 52-53 0.35497429592973617 1105 54-55 0.4181720895628942 1105 56-57 0.4530516958221682 1105 58-59 0.4715498246917633 1105 60-61 0.4085103665980796 1105 62-63 0.41578631769204577 1105 64-65 0.49572194934608405 1105 66-67 0.5410390252001278 1105 68-69 0.5648408690864031 1105 70-71 0.5552838889702443 1105 72-73 0.5112031142055855 1105 74-75 0.5307353129711245 1105 76-77 0.47452204468651615 1105 78-79 0.4897751431900801 1105 80-81 0.6006715940552638 1105 82-83 0.44743223454167946 1105 84-85 0.508861730321847 1105 86-87 0.6164681973253288 1105 88-89 0.5187819726426497 1105 90-91 0.5676175172852567 1105 92-93 0.5970088396334035 1105 94-95 0.5871641274394399 1105 96-97 0.6077264782446505 1105 98-99 0.6030970223822223 1105 100-101 0.74301207756859 1106 1 0.04421979376091656 1106 2 0.05099242957889061 1106 3 0.1056224149359366 1106 4 0.0797016895617162 1106 5 0.05721272712111869 1106 6 0.10933979650666004 1106 7 0.1297614279790693 1106 8 0.11970654790719948 1106 9 0.20299657889236045 1106 10-11 0.09818407506408988 1106 12-13 0.15806029602753568 1106 14-15 0.141912931987207 1106 16-17 0.16850515354362727 1106 18-19 0.16424083496550423 1106 20-21 0.2357207047686387 1106 22-23 0.15941099850114426 1106 24-25 0.1705238921975436 1106 26-27 0.30417636316594354 1106 28-29 0.2563639263579134 1106 30-31 0.2967580089253943 1106 32-33 0.2663083244159594 1106 34-35 0.13156541082626205 1106 36-37 0.22549429932620768 1106 38-39 0.11278508293110434 1106 40-41 0.25069542129134703 1106 42-43 0.3105078247501112 1106 44-45 0.2705095534393962 1106 46-47 0.04904932557966646 1106 48-49 0.14426804222601675 1106 50-51 0.11280165975797729 1106 52-53 0.27631667903682455 1106 54-55 0.18848021281218763 1106 56-57 0.20041642530796366 1106 58-59 0.19096374410568018 1106 60-61 0.06621537008047795 1106 62-63 0.175899274461095 1106 64-65 0.0985287024335264 1106 66-67 0.23283285093341988 1106 68-69 0.12414643619035814 1106 70-71 0.17582831484784123 1106 72-73 -0.015367878059819873 1106 74-75 0.1016753199985061 1106 76-77 -0.03288259607179356 1106 78-79 0.1479174808784265 1106 80-81 0.24822398877632423 1106 82-83 0.2471238698935494 1106 84-85 0.38939295973030497 1106 86-87 0.36769065886021224 1106 88-89 0.29314058937373133 1106 90-91 0.36827841362592295 1106 92-93 0.35379572291528305 1106 94-95 0.45457854932054076 1106 96-97 0.42179345837216076 1106 98-99 0.42469399184348333 1106 100-101 0.4738603888049937 1107 1 0.1550044847699148 1107 2 0.22608475923748372 1107 3 0.15129635646705353 1107 4 0.21361352427464908 1107 5 0.18575246387784006 1107 6 0.16068506373656533 1107 7 0.24347878561951575 1107 8 0.20561375125546277 1107 9 0.25168426108885456 1107 10-11 0.1956206851466149 1107 12-13 0.14455790614611175 1107 14-15 0.3206336809871928 1107 16-17 0.2055314216982964 1107 18-19 0.2791543909296408 1107 20-21 0.3223530050997283 1107 22-23 0.28850643194540027 1107 24-25 0.3260248534320809 1107 26-27 0.2979784335301616 1107 28-29 0.22574066238606605 1107 30-31 0.3124937075748093 1107 32-33 0.3630703096650123 1107 34-35 0.3400737827473108 1107 36-37 0.3356776423431498 1107 38-39 0.3049423229597039 1107 40-41 0.29716211931446423 1107 42-43 0.329435761084774 1107 44-45 0.34435697328131454 1107 46-47 0.17563368575294902 1107 48-49 0.11366942744337649 1107 50-51 0.11016340002426972 1107 52-53 0.13818719198309282 1107 54-55 0.2965767588235906 1107 56-57 0.33007304701082063 1107 58-59 0.2946459892276181 1107 60-61 0.32247421004268517 1107 62-63 0.33807140461216534 1107 64-65 0.30379478372386615 1107 66-67 0.3286261817347622 1107 68-69 0.3658840950371953 1107 70-71 0.2502463969564701 1107 72-73 0.2238921599871233 1107 74-75 0.24609642875928017 1107 76-77 0.193362502386627 1107 78-79 0.2931490784561035 1107 80-81 0.37882750824456224 1107 82-83 0.45966586364406226 1107 84-85 0.5304614433943478 1107 86-87 0.5378969994030136 1107 88-89 0.5205850721054652 1107 90-91 0.487852133565859 1107 92-93 0.6807315855572575 1107 94-95 0.559065875529086 1107 96-97 0.49982247901963106 1107 98-99 0.5038020014046829 1107 100-101 0.5281364781692055 1108 1 0.032453961384096175 1108 2 0.033016635531048166 1108 3 0.0166237196579182 1108 4 0.07181606737266577 1108 5 0.028085442499822477 1108 6 0.033528086838074955 1108 7 0.05748047382216015 1108 8 0.05394469768831556 1108 9 0.026588993883265744 1108 10-11 0.10632983701086829 1108 12-13 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-0.896707223637911 2316 92-93 -0.744810740494561 2316 94-95 -0.7833670496585299 2316 96-97 -0.7164896321696226 2316 98-99 -0.8277646966893073 2316 100-101 -0.8107892806675565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75946.0 3 11850.0 4 8810.0 5 7370.0 6 7408.0 7 8005.0 8 8243.0 9 8275.0 10 8553.0 11 8674.0 12 8734.0 13 9044.0 14 9510.0 15 10169.0 16 11549.0 17 14089.0 18 12979.0 19 11425.0 20 12006.0 21 13240.0 22 14645.0 23 17059.0 24 20265.0 25 23668.0 26 27115.0 27 31896.0 28 38644.0 29 47419.0 30 59330.0 31 73859.0 32 96824.0 33 128571.0 34 177823.0 35 260094.0 36 416848.0 37 834539.0 38 872737.0 39 262.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.48592821980491 23.060093477307717 13.18284774163027 23.2711305612571 2 13.888232626869307 21.82702367972718 43.352228788103346 20.932514905300167 3 17.387782676487827 30.158008588769448 30.578408104774418 21.87580062996831 4 11.022810946507395 18.655572521682682 43.190949929316496 27.130666602493424 5 11.760393494197956 39.09195226887205 36.84740947696498 12.300244759965018 6 25.78391578285048 41.888177088209254 20.626639592871793 11.701267536068476 7 24.06469654820854 32.59100501632146 27.447345939532386 15.896952495937612 8 22.653916666202004 38.39670818027532 22.267677815580267 16.681697337942413 9 25.512307199725782 16.151803812615608 23.648905040298143 34.68698394736047 10-11 22.610367296952628 27.842438732850383 31.348891972632515 18.198301997564474 12-13 23.00677070662042 25.53779589742897 30.511349613041688 20.94408378290892 14-15 20.6025926230278 27.435771388993242 27.588308837340346 24.37332715063862 16-17 19.860596857581577 29.437001953446 29.544036472151653 21.158364716820767 18-19 19.549473270244132 30.29995495026186 30.13381386387349 20.01675791562052 20-21 20.250789660502477 29.250982471781906 29.624103112067964 20.874124755647657 22-23 20.353152787238184 29.737544816883577 29.052404462304516 20.856897933573727 24-25 20.28605333008166 29.458735812264592 29.676154773187726 20.57905608446603 26-27 20.468428693722657 29.20528883980728 29.0530354276786 21.273247038791457 28-29 20.182660073714366 29.7856889422878 29.241400602263784 20.79025038173405 30-31 21.205410924270364 29.531806084090956 28.82180862849551 20.44097436314317 32-33 20.920258596022805 29.632393703611204 28.78659195645341 20.660755743912578 34-35 21.226951788669446 28.905081384261727 29.3137708633103 20.554195963758524 36-37 21.427114548388733 29.258451340977505 29.03855257130129 20.275881539332474 38-39 21.832414422753256 28.764713598947257 29.64520376806652 19.757668210232968 40-41 21.062280656575016 28.39164711770799 30.128815495247867 20.417256730469127 42-43 21.440676113246855 28.80606806826107 29.078043531628172 20.6752122868639 44-45 20.852508781833272 29.411037926925342 29.17482095537131 20.56163233587007 46-47 21.47638918563029 28.619769803536016 28.718248547050678 21.18559246378302 48-49 21.590569743670134 27.80959102748874 29.453609586809865 21.146229642031262 50-51 21.241984006900648 28.636362029469158 28.64266641213458 21.478987551495614 52-53 20.440428271602194 28.66331546144924 29.301754061068053 21.594502205880513 54-55 21.162219676369936 28.927415880279455 28.643149213063257 21.267215230287352 56-57 21.357321908520564 28.188974408213387 29.099310081985248 21.3543936012808 58-59 20.509895963624057 28.136247921479757 30.223023382433006 21.130832732463176 60-61 20.850981443334156 29.448713991603054 28.411056615087844 21.289247949974946 62-63 21.25004523680696 28.603637598303628 28.08576074775044 22.06055641713897 64-65 20.235699854570555 28.608999561328535 28.645246593087936 22.51005399101297 66-67 20.845948068402937 27.8821056756269 28.917319264548258 22.354626991421906 68-69 20.083154005058596 28.306847455254207 28.72479397898179 22.885204560705407 70-71 20.044120733553157 28.067731606273856 29.25642341431646 22.631724245856528 72-73 20.46090991100622 28.039524138270604 29.211346289111567 22.288219661611606 74-75 20.68453921569495 28.64228326429894 28.451240421680545 22.221937098325565 76-77 20.211267564053664 28.60496333970613 29.1893493255427 21.994419770697508 78-79 20.281439367562303 28.309810613482995 28.765474211230575 22.643275807724127 80-81 20.282368466980735 28.6524147239401 29.189710041236005 21.87550676784316 82-83 20.901805709490123 27.74278295159768 29.288484635012864 22.066926703899338 84-85 21.335607173010864 27.519050542471707 28.86883390539762 22.276508379119807 86-87 20.51413519526762 28.461169965778545 28.834595219174254 22.19009961977958 88-89 21.364949373729818 27.067165209804223 29.143887333290873 22.423998083175082 90-91 21.174718879728935 26.950506092244726 29.27909381325785 22.595681214768486 92-93 20.459239353391272 27.795285269680566 29.26982583770555 22.475649539222616 94-95 20.227069175134577 27.025459364692562 29.4147158100125 23.33275565016036 96-97 20.4881267731377 27.152376849396948 29.03114609861217 23.328350278853176 98-99 20.192015070839265 27.7064360389379 29.166188478067124 22.935360412155713 100-101 20.85392106009466 26.467012371003744 28.754039054108265 23.925027514793335 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 37.0 1 25.5 2 18.5 3 27.0 4 27.5 5 27.0 6 40.0 7 69.5 8 95.5 9 104.0 10 110.0 11 122.5 12 165.0 13 255.5 14 341.5 15 478.0 16 648.0 17 752.0 18 815.5 19 888.0 20 1001.0 21 1044.5 22 1081.5 23 1248.5 24 1379.0 25 1508.0 26 1822.5 27 2378.0 28 3672.0 29 7094.5 30 13472.0 31 24370.5 32 42750.5 33 72023.5 34 113926.0 35 153146.5 36 195668.5 37 249521.0 38 273874.5 39 253615.5 40 223576.0 41 193525.5 42 167375.0 43 150568.5 44 132970.5 45 116489.0 46 103355.0 47 93741.0 48 86064.0 49 77933.5 50 70667.5 51 66830.5 52 63313.0 53 59942.0 54 56867.5 55 51252.5 56 46018.5 57 39267.0 58 32598.0 59 29862.0 60 25769.5 61 20413.0 62 16121.5 63 13399.0 64 10757.5 65 7679.5 66 5676.0 67 4504.5 68 3761.0 69 3141.5 70 2589.5 71 2080.5 72 1684.0 73 1425.0 74 1183.5 75 954.5 76 809.5 77 673.5 78 530.5 79 441.5 80 369.5 81 305.0 82 263.5 83 224.5 84 179.5 85 133.0 86 119.0 87 107.0 88 80.0 89 57.0 90 52.5 91 42.5 92 25.5 93 28.0 94 25.0 95 13.5 96 5.0 97 2.0 98 3.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.575973073332557E-4 2 0.0038738339246310394 3 1.173889068070012E-4 4 9.9780570785951E-4 5 0.001672791921999767 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.336806675437574E-5 12-13 1.173889068070012E-4 14-15 0.0034336255241047844 16-17 0.004842292405788799 18-19 0.0038738339246310394 20-21 0.0 22-23 0.0 24-25 1.467361335087515E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 1.9075697356137696E-4 42-43 1.467361335087515E-5 44-45 4.842292405788799E-4 46-47 0.6896011330377285 48-49 0.4972447356211062 50-51 0.3818954610698766 52-53 0.3224526533854814 54-55 0.3130321936142196 56-57 0.2820121749904695 58-59 0.2825257514577501 60-61 0.2600164285775076 62-63 0.2552034833984206 64-65 0.25174051064761405 66-67 0.2400162935802648 68-69 0.22070581841051312 70-71 0.23306100085195 72-73 0.7615165120703676 74-75 0.5029527712145966 76-77 0.5916694375339877 78-79 0.32931990443369097 80-81 0.2851963490876094 82-83 0.2675733394532083 84-85 0.2588572131227885 86-87 0.23482183445405502 88-89 0.23787394603103704 90-91 0.2477639614295269 92-93 0.2062523092599011 94-95 0.17486545030237916 96-97 0.13532006232177063 98-99 0.14319979269119057 100-101 0.13975149355373492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3407477.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.64107841676057 #Duplication Level Percentage of deduplicated Percentage of total 1 79.15681997574178 34.544889877827465 2 11.899771701896055 10.386377399679885 3 3.8702110667134644 5.067005539555707 4 1.6954528550486014 2.9596556399638643 5 0.8795037498176516 1.9191246056813556 6 0.500722784605357 1.3111249384812662 7 0.3314029727632498 1.0123948185335987 8 0.23063343636848602 0.8052073505667245 9 0.15098511464561332 0.5930237905211516 >10 0.982873088804594 8.345713847805222 >50 0.1380637140810688 4.241181400334199 >100 0.1390541030310972 12.218125859993096 >500 0.014041682612962509 4.25989171702279 >1k 0.009113592080457467 7.450398681320303 >5k 8.776051633033117E-4 2.492392988362502 >10k+ 4.725566263940909E-4 2.393491544350954 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 14356 0.4213087865303273 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 13566 0.39812447743594453 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 11693 0.3431571218235662 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 10723 0.31469031192286845 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 10421 0.30582744945893986 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 10314 0.3026872962018526 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 10169 0.2984319483300988 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 9336 0.2739857084875408 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 9327 0.273721583447225 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 7335 0.21526190785733845 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 7214 0.21171089342642666 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 7172 0.21047830990495314 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 6762 0.1984459469572355 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 5785 0.16977370646962547 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 5611 0.16466728902352093 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 5447 0.15985434384443387 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 5304 0.15565769042608357 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 5172 0.15178385650145254 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 5126 0.15043388407317201 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 5008 0.14697091132236548 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 4841 0.14206992446317318 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 4479 0.1314462283971396 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 4467 0.13109406167671858 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 4341 0.12739631111229804 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 4257 0.12493114406935102 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 4030 0.11826932360805369 No Hit GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT 4022 0.1180345457944397 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 3989 0.11706608731328193 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 3979 0.11677261504626443 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 3967 0.11642044832584345 No Hit GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA 3912 0.11480635085724716 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 3635 0.10667716906086232 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 3622 0.10629565511373958 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.6412504031575267E-4 0.0 0.0 0.0 0.0 7 2.6412504031575267E-4 0.0 0.0 1.1738890680700119E-4 0.0 8 2.6412504031575267E-4 0.0 0.0 1.1738890680700119E-4 0.0 9 2.6412504031575267E-4 0.0 0.0 1.1738890680700119E-4 0.0 10-11 2.6412504031575267E-4 0.0 0.0 1.6140974685962663E-4 0.0 12-13 2.6412504031575267E-4 0.0 0.0 1.7608336021050178E-4 1.4673613350875148E-5 14-15 2.6412504031575267E-4 0.0 0.0 1.7608336021050178E-4 4.4020840052625445E-5 16-17 4.989028539297551E-4 0.0 0.0 1.7608336021050178E-4 5.8694453403500594E-5 18-19 4.989028539297551E-4 0.0 0.0 1.7608336021050178E-4 5.8694453403500594E-5 20-21 4.989028539297551E-4 0.0 0.0 1.7608336021050178E-4 8.804168010525089E-5 22-23 4.989028539297551E-4 0.0 0.0 2.9347226701750297E-4 8.804168010525089E-5 24-25 4.989028539297551E-4 0.0 0.0 8.070487342981332E-4 8.804168010525089E-5 26-27 4.989028539297551E-4 0.0 0.0 0.0021276739358768964 1.1738890680700119E-4 28-29 4.989028539297551E-4 0.0 0.0 0.005649341140086932 1.1738890680700119E-4 30-31 5.135764672806302E-4 0.0 0.0 0.020000134997242826 1.1738890680700119E-4 32-33 5.429236939823805E-4 0.0 0.0 0.07110833029834097 1.1738890680700119E-4 34-35 6.162917607367563E-4 0.0 0.0 0.1536914262370663 1.1738890680700119E-4 36-37 6.162917607367563E-4 0.0 0.0 0.2842278906064516 1.4673613350875148E-4 38-39 6.162917607367563E-4 0.0 0.0 0.4649334390224791 1.4673613350875148E-4 40-41 6.162917607367563E-4 0.0 0.0 0.6518899467259793 1.4673613350875148E-4 42-43 6.162917607367563E-4 0.0 0.0 0.8545912415549687 1.4673613350875148E-4 44-45 6.309653740876314E-4 0.0 0.0 1.0930374585066898 1.4673613350875148E-4 46-47 6.456389874385065E-4 0.0 0.0 1.381212551104527 1.4673613350875148E-4 48-49 6.456389874385065E-4 0.0 0.0 1.6686099421947675 1.4673613350875148E-4 50-51 6.749862141402569E-4 0.0 0.0 1.9501085407179564 1.4673613350875148E-4 52-53 6.749862141402569E-4 0.0 0.0 2.2393988279304597 1.4673613350875148E-4 54-55 6.749862141402569E-4 0.0 0.0 2.547647423592294 1.4673613350875148E-4 56-57 6.749862141402569E-4 0.0 0.0 2.9108927220932084 1.4673613350875148E-4 58-59 6.749862141402569E-4 0.0 0.0 3.3323482447570445 1.4673613350875148E-4 60-61 7.190070541928823E-4 0.0 0.0 3.7544053855682664 1.4673613350875148E-4 62-63 7.923751209472581E-4 0.0 0.0 4.169830053144893 1.4673613350875148E-4 64-65 7.923751209472581E-4 0.0 0.0 4.610977564925603 1.6140974685962663E-4 66-67 7.923751209472581E-4 0.0 0.0 5.0814429561813625 1.7608336021050178E-4 68-69 7.923751209472581E-4 0.0 0.0 5.595254201275607 1.7608336021050178E-4 70-71 7.923751209472581E-4 0.0 0.0 6.131046519169462 1.7608336021050178E-4 72-73 8.217223476490083E-4 0.0 0.0 6.6995756684491194 1.7608336021050178E-4 74-75 8.217223476490083E-4 0.0 0.0 7.237128819945079 1.7608336021050178E-4 76-77 8.217223476490083E-4 0.0 0.0 7.833508487364698 1.7608336021050178E-4 78-79 8.217223476490083E-4 0.0 0.0 8.493248817233397 1.7608336021050178E-4 80-81 8.217223476490083E-4 0.0 0.0 9.201808258720455 1.9075697356137693E-4 82-83 8.363959609998835E-4 0.0 0.0 9.949766352054613 2.0543058691225208E-4 84-85 8.510695743507587E-4 0.0 0.0 10.656682348846376 2.3477781361400238E-4 86-87 8.510695743507587E-4 0.0 0.0 11.433107252081232 2.3477781361400238E-4 88-89 8.80416801052509E-4 0.0 0.0 12.230089887620665 2.3477781361400238E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 3830 0.0 52.317863 3 TACCTGG 4045 0.0 49.187176 2 CCTGGGG 3245 0.0 44.961876 4 GTACCTG 4765 0.0 42.152573 1 CTGGGGG 2945 0.0 41.659737 5 GTACCCG 355 0.0 41.367043 1 TGGGGGG 5885 0.0 32.357048 6 TATTCGC 860 0.0 29.74517 9 CTGTGCG 1430 0.0 27.826874 9 CTAACGC 580 0.0 27.76894 3 GTTATAA 4690 0.0 27.473705 1 GGGGGGA 6050 0.0 27.403244 7 TAACGCC 615 0.0 26.958849 4 GTACATG 9050 0.0 25.858273 1 ACATGGG 8740 0.0 25.311264 3 CATGGGG 5455 0.0 24.922726 4 TCTAACG 670 0.0 24.746534 2 GGGGAAT 6570 0.0 24.29885 9 ATGGGGG 4755 0.0 24.108316 5 ATAATGA 4580 0.0 23.99556 4 >>END_MODULE