FastQCFastQC Report
Sat 18 Jun 2016
SRR1931067_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931067_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144926
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA34320.2997573642314001No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA30870.26962441240743945No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC28640.250147171083546No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC25940.22656486096044637No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC24650.21529775723496541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20730.18105973661179847No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT16090.140533099955805No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.13834955272218466No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC15340.13398245825494398No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT14740.12874194489425517No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC14110.12323940586553192No Hit
GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA14060.12280269641880784No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA13960.1219292775253597No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC13880.12123054241060122No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT13820.12070649107453232No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13620.11895965328763607No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG13280.1159900290499124No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA12160.10620773744329329No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC11610.10140393352932853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17850.062.3613322
ACCTGGG19000.059.085443
GTACCTG19250.058.810241
CCTGGGG17250.050.8005454
CTGGGGG15150.043.457925
ATAACGC1550.039.7280653
TATCACG851.6635611E-533.436292
GTACACG2000.033.1576541
TGGGGGG32700.030.70696
TAACGCA2101.8189894E-1229.3230954
GTATAAC3000.028.4208471
GTAGCGC909.093985E-426.31563
GTCCTAC6500.026.2346271
CTATTGA15450.025.1392429
GCATCGT7200.024.9976378
GTACATG43250.024.7518731
GTACTAG8050.024.7137811
TAGGCAT8850.024.6196215
CATGGGG27350.024.59334
GAGTACC4250.024.5199451
Produced by FastQC (version 0.11.5)