##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931067_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1144926 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.454640736606557 33.0 33.0 33.0 27.0 33.0 2 31.366541593081124 33.0 33.0 33.0 27.0 33.0 3 31.392956400675676 33.0 33.0 33.0 27.0 33.0 4 35.03859550748258 37.0 37.0 37.0 33.0 37.0 5 34.99244317973389 37.0 37.0 37.0 33.0 37.0 6 35.10373508855594 37.0 37.0 37.0 33.0 37.0 7 35.09255707355759 37.0 37.0 37.0 33.0 37.0 8 35.096987927604054 37.0 37.0 37.0 33.0 37.0 9 35.12874631198872 37.0 37.0 37.0 33.0 37.0 10-11 35.02327486667261 37.0 37.0 37.0 33.0 37.0 12-13 34.9372444157963 37.0 37.0 37.0 33.0 37.0 14-15 36.652947002688386 40.0 37.0 40.0 33.0 40.0 16-17 36.583456048687864 40.0 37.0 40.0 33.0 40.0 18-19 36.52337880352093 40.0 37.0 40.0 33.0 40.0 20-21 36.42712935159128 40.0 37.0 40.0 33.0 40.0 22-23 36.320640809973746 40.0 37.0 40.0 33.0 40.0 24-25 36.2004024714261 40.0 37.0 40.0 33.0 40.0 26-27 36.03672682776005 40.0 37.0 40.0 33.0 40.0 28-29 35.901667444009476 40.0 37.0 40.0 33.0 40.0 30-31 35.746468330704346 40.0 37.0 40.0 30.0 40.0 32-33 35.569892289982064 37.0 37.0 40.0 27.0 40.0 34-35 35.420120164971365 37.0 37.0 40.0 27.0 40.0 36-37 35.185232058665804 37.0 37.0 40.0 27.0 40.0 38-39 34.97675526627921 37.0 37.0 40.0 27.0 40.0 40-41 34.732500179050874 37.0 37.0 40.0 27.0 40.0 42-43 34.516104970976286 37.0 37.0 40.0 22.0 40.0 44-45 34.19526807846097 37.0 33.0 40.0 22.0 40.0 46-47 34.004281936125125 37.0 33.0 40.0 22.0 40.0 48-49 33.95310395606353 37.0 33.0 40.0 18.5 40.0 50-51 33.142166829996 37.0 33.0 38.5 15.0 40.0 52-53 33.28724825883943 37.0 33.0 38.5 18.5 40.0 54-55 33.74897591634743 37.0 33.0 40.0 22.0 40.0 56-57 33.66917730927588 37.0 33.0 40.0 15.0 40.0 58-59 33.48446624498003 37.0 33.0 40.0 15.0 40.0 60-61 33.47537963152203 37.0 33.0 40.0 15.0 40.0 62-63 33.27452865949415 37.0 33.0 40.0 15.0 40.0 64-65 33.034642413570836 37.0 33.0 40.0 15.0 40.0 66-67 32.76546606505573 37.0 33.0 40.0 15.0 40.0 68-69 32.50580168499973 37.0 33.0 38.5 6.0 40.0 70-71 32.257598744373 37.0 33.0 37.0 6.0 40.0 72-73 31.65707041328435 37.0 33.0 37.0 2.0 40.0 74-75 31.479546276353233 37.0 33.0 37.0 2.0 40.0 76-77 31.125805510574484 37.0 33.0 37.0 2.0 38.5 78-79 30.869245697975238 37.0 33.0 37.0 2.0 37.0 80-81 30.64217163379991 37.0 33.0 37.0 2.0 37.0 82-83 30.383004229094283 37.0 33.0 37.0 2.0 37.0 84-85 29.996581001741596 35.0 30.0 37.0 2.0 37.0 86-87 29.821050006725326 33.0 33.0 37.0 2.0 37.0 88-89 29.63424142695685 33.0 30.0 37.0 2.0 37.0 90-91 29.345536742112593 33.0 27.0 37.0 2.0 37.0 92-93 29.060168954150747 33.0 27.0 37.0 2.0 37.0 94-95 28.798971287227296 33.0 27.0 37.0 2.0 37.0 96-97 28.48129267743068 33.0 27.0 37.0 2.0 37.0 98-99 28.14186550047776 33.0 27.0 37.0 2.0 37.0 100-101 27.432277282549265 33.0 27.0 37.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21620454003668144 1101 2 0.2105241490257974 1101 3 0.06464467381375272 1101 4 0.1540421388117892 1101 5 -0.025437876854013552 1101 6 0.10646412858443455 1101 7 -0.04072695553835359 1101 8 0.03685610220827584 1101 9 0.055658047136816435 1101 10-11 0.11059842681402188 1101 12-13 0.17533459718763567 1101 14-15 0.07273939975760157 1101 16-17 -0.08219941913837658 1101 18-19 -0.009491135706049647 1101 20-21 -0.0733432791105102 1101 22-23 0.040022991307246514 1101 24-25 -0.0055471525217711815 1101 26-27 0.023978374531608893 1101 28-29 -0.0015007027541358298 1101 30-31 -0.12372126630297942 1101 32-33 0.0020162412936244323 1101 34-35 -0.13137609450613041 1101 36-37 -0.017368650465591884 1101 38-39 0.09393081463832686 1101 40-41 0.15491385015143777 1101 42-43 0.008453889195735087 1101 44-45 -0.21730601089227264 1101 46-47 0.1627762281208618 1101 48-49 0.2809333044850604 1101 50-51 0.37144892053089507 1101 52-53 0.1980146408026755 1101 54-55 0.24186821099476674 1101 56-57 0.22265463852645695 1101 58-59 0.052651118640099526 1101 60-61 0.22138397452178538 1101 62-63 0.16911123198533318 1101 64-65 0.30366326370700847 1101 66-67 0.504071346091159 1101 68-69 0.4708345542901995 1101 70-71 0.48976262600574216 1101 72-73 0.3846555606895734 1101 74-75 0.3273407942964077 1101 76-77 0.19253725687832102 1101 78-79 0.177570448329746 1101 80-81 0.2447043177933672 1101 82-83 0.2621047270741528 1101 84-85 0.17568242370009912 1101 86-87 0.2760749967559022 1101 88-89 0.1189118270439593 1101 90-91 -0.03388838729079424 1101 92-93 0.14922295867480528 1101 94-95 0.0864037306504386 1101 96-97 -0.3426665703045586 1101 98-99 -0.06644000004849104 1101 100-101 -0.045219940986321205 1102 1 -0.18102239656169417 1102 2 -0.23858937988679685 1102 3 -0.16910941759126885 1102 4 -0.19282263267566435 1102 5 -0.3035397679923335 1102 6 -0.23803368933288738 1102 7 -0.31828332600750286 1102 8 -0.2633863514224686 1102 9 -0.14419799601993333 1102 10-11 -0.19630998632696617 1102 12-13 -0.13988694481745512 1102 14-15 -0.28212634154437666 1102 16-17 -0.1720754647196614 1102 18-19 -0.04574553017810956 1102 20-21 -0.24092647101254983 1102 22-23 -0.1649510964609746 1102 24-25 -0.16572490133981432 1102 26-27 -0.267496349677387 1102 28-29 -0.13181271027018937 1102 30-31 -0.1547826070715601 1102 32-33 -0.058513822956577144 1102 34-35 -0.11498395606511025 1102 36-37 -0.18182343286346025 1102 38-39 -0.19705142563322653 1102 40-41 -0.17956457147542437 1102 42-43 -0.20052295648252993 1102 44-45 -0.24414941615351893 1102 46-47 -0.3173584851777207 1102 48-49 -0.20201538626533022 1102 50-51 -0.297895848532022 1102 52-53 -0.13625996824084297 1102 54-55 -0.0365329588440062 1102 56-57 0.019920973839830936 1102 58-59 0.10616063494511252 1102 60-61 0.09876608144383425 1102 62-63 0.1308543220568552 1102 64-65 0.062295370629058766 1102 66-67 0.2545562533526464 1102 68-69 0.180969116055266 1102 70-71 -0.026753012253450947 1102 72-73 -0.11839253604942002 1102 74-75 -0.1687178188688243 1102 76-77 -0.10975468759979634 1102 78-79 0.09975571321033883 1102 80-81 0.14735463904442625 1102 82-83 -0.03542906490063302 1102 84-85 0.05489202486387157 1102 86-87 0.02492182667468157 1102 88-89 -0.08281262440469916 1102 90-91 0.09160333897905915 1102 92-93 -0.04408986712225982 1102 94-95 0.029437734772148616 1102 96-97 0.0928703129540196 1102 98-99 0.0012022899260486497 1102 100-101 -0.0016586002177447767 1103 1 -0.006981300671281332 1103 2 -0.1116144940420476 1103 3 -0.24719160937208784 1103 4 -0.310117153223608 1103 5 -0.18363109219324514 1103 6 -0.1472231870497822 1103 7 -0.05658241276549347 1103 8 -0.18770141991561928 1103 9 -0.13575050743545347 1103 10-11 -0.08814788587034172 1103 12-13 -0.10344858865307316 1103 14-15 -0.058901455699626126 1103 16-17 -0.1561964692858666 1103 18-19 -0.07572269912787988 1103 20-21 -0.2740143705559319 1103 22-23 -0.262097215182429 1103 24-25 -0.1796290109288563 1103 26-27 -0.12012192045364145 1103 28-29 -0.03894170570398359 1103 30-31 -0.15682598606699116 1103 32-33 -0.22907889144973126 1103 34-35 -0.22540633049433012 1103 36-37 -0.1969261725894924 1103 38-39 -0.17658909686610258 1103 40-41 -0.16580886371286851 1103 42-43 -0.1889133674414296 1103 44-45 -0.43159804629050313 1103 46-47 -0.3575925034425609 1103 48-49 -0.4746124182288014 1103 50-51 -0.2859552092626174 1103 52-53 -0.25104878102623474 1103 54-55 -0.2229142373828239 1103 56-57 -0.13152309008710716 1103 58-59 -0.3920870819498674 1103 60-61 -0.3012967039442884 1103 62-63 -0.3814344907285303 1103 64-65 -0.35026741475906675 1103 66-67 -0.25181360966104904 1103 68-69 -0.26724435426437054 1103 70-71 -0.26561716750458686 1103 72-73 -0.5371254127617533 1103 74-75 -0.3362064033437093 1103 76-77 -0.39385286111577855 1103 78-79 -0.30401141007733656 1103 80-81 -0.30523668515648694 1103 82-83 -0.20838568590519913 1103 84-85 -1.250984237977093E-4 1103 86-87 -0.2171329678458669 1103 88-89 -0.17148271572890295 1103 90-91 -0.16254620439993772 1103 92-93 -0.5947519675788797 1103 94-95 -0.2807791602050216 1103 96-97 -0.2151776322514607 1103 98-99 -0.29410592925203005 1103 100-101 -0.13794855455564203 1104 1 0.06553902453196869 1104 2 0.03623103441323394 1104 3 -0.03570786953468996 1104 4 -0.049548047532553596 1104 5 0.008787606993742259 1104 6 0.019443479616882087 1104 7 -0.010444201383378982 1104 8 -0.055790850809927406 1104 9 0.08691209419055923 1104 10-11 -0.13591211351262444 1104 12-13 -0.14550143418153283 1104 14-15 -0.09998885407360802 1104 16-17 -0.07778183513952541 1104 18-19 -0.06643408124170236 1104 20-21 -0.030782663238859698 1104 22-23 -0.09487160542633433 1104 24-25 0.027555948420740606 1104 26-27 -0.03963411557559482 1104 28-29 0.026688375596826575 1104 30-31 -0.034204034847476805 1104 32-33 -0.11524759063672008 1104 34-35 -0.1624286882179078 1104 36-37 -0.0978204815325725 1104 38-39 -0.14605047897992307 1104 40-41 -0.11926414826571374 1104 42-43 0.15968419353417573 1104 44-45 0.009580815498111406 1104 46-47 -0.47803965791410974 1104 48-49 -0.4672140442450683 1104 50-51 -0.29961374584798506 1104 52-53 -0.17541869972541946 1104 54-55 -0.07989594469989214 1104 56-57 0.08820251966898951 1104 58-59 0.22072795870053596 1104 60-61 -0.004071094188198288 1104 62-63 -0.04936131999682658 1104 64-65 -0.06935278061273209 1104 66-67 -0.10860222754723026 1104 68-69 -0.011563459955425515 1104 70-71 -0.03591188295174419 1104 72-73 -0.1405908599162231 1104 74-75 -0.16407225700224615 1104 76-77 -0.21526546274179736 1104 78-79 -0.09379799544318956 1104 80-81 0.20094217663212888 1104 82-83 0.16142122466390418 1104 84-85 0.23140945442173333 1104 86-87 0.37683044678827926 1104 88-89 0.21911687776703204 1104 90-91 0.13261639722607654 1104 92-93 0.316853723478026 1104 94-95 0.10072490483563001 1104 96-97 0.2696292783176446 1104 98-99 0.5529570788780482 1104 100-101 0.4733929088589903 1105 1 -0.059756341722184914 1105 2 -0.07211531506011326 1105 3 0.08948737256551453 1105 4 0.15827365137409544 1105 5 0.012924737034992972 1105 6 0.02896728914069513 1105 7 -0.09168093493298102 1105 8 -0.11762752829000078 1105 9 -0.13991717410212345 1105 10-11 -0.07070125204440103 1105 12-13 -0.1444461255988898 1105 14-15 -0.09389529806415453 1105 16-17 -0.14521945779161882 1105 18-19 0.012992407604471623 1105 20-21 0.06679433223552422 1105 22-23 0.04218234146781441 1105 24-25 -0.05499641651178422 1105 26-27 -0.04976067250618854 1105 28-29 -0.014939242649795403 1105 30-31 0.010329186346794472 1105 32-33 -0.028826428395547055 1105 34-35 -0.15678769338431664 1105 36-37 -0.0108506389277494 1105 38-39 0.008581264381845699 1105 40-41 -0.16839507060942083 1105 42-43 -0.13508331818034947 1105 44-45 -0.0554157802806543 1105 46-47 0.04545347356893359 1105 48-49 -0.05584394532240111 1105 50-51 0.09148321438269846 1105 52-53 0.3180111532923249 1105 54-55 0.4478965179537937 1105 56-57 0.17977206590632733 1105 58-59 0.33702416599759033 1105 60-61 0.12419795943829826 1105 62-63 0.04120097725175498 1105 64-65 0.07864473630825586 1105 66-67 0.007784091488375111 1105 68-69 0.13463782964367255 1105 70-71 0.07077240556601794 1105 72-73 0.04632491564547081 1105 74-75 -0.07525812748164284 1105 76-77 0.05901864955745495 1105 78-79 0.024016094027757617 1105 80-81 0.04982078610788321 1105 82-83 0.05607859980908714 1105 84-85 -0.016896444893419016 1105 86-87 0.2002274590835249 1105 88-89 0.11584281793284035 1105 90-91 0.20357038016492268 1105 92-93 0.2726248797117563 1105 94-95 0.27977092189678743 1105 96-97 0.2622341083068349 1105 98-99 0.1625710001404137 1105 100-101 0.16810234856422568 1106 1 0.07667603046545679 1106 2 0.1356644735581689 1106 3 0.1792704993593759 1106 4 0.0840344118505243 1106 5 -0.058714837388194496 1106 6 0.08627489092934582 1106 7 0.13486459437344678 1106 8 0.1138224680580251 1106 9 0.1319114914662478 1106 10-11 0.18992734751790152 1106 12-13 0.17125898960063068 1106 14-15 0.16626260251015168 1106 16-17 0.254575084805289 1106 18-19 0.09349613667662027 1106 20-21 0.1804735481700348 1106 22-23 0.15876014341174738 1106 24-25 0.2832684355291306 1106 26-27 0.24349697026573836 1106 28-29 0.3558180416875629 1106 30-31 0.4766322786227377 1106 32-33 0.2675211909225865 1106 34-35 0.19712181338265822 1106 36-37 0.0927937615126524 1106 38-39 0.14125480758200837 1106 40-41 0.09260684194336477 1106 42-43 0.052433420037971246 1106 44-45 0.0897116736435919 1106 46-47 0.07288251028616344 1106 48-49 0.09214760373714626 1106 50-51 0.12815242390597348 1106 52-53 0.33569888509128276 1106 54-55 0.2558824402668236 1106 56-57 0.4545196062335961 1106 58-59 0.4200950981709468 1106 60-61 0.30329486662132865 1106 62-63 0.38658582777778605 1106 64-65 0.20180150341775516 1106 66-67 0.36340055837849405 1106 68-69 0.4312681388712676 1106 70-71 0.31451806039217445 1106 72-73 0.0351789529713642 1106 74-75 0.10822385475623619 1106 76-77 0.23965276765961718 1106 78-79 0.3972756574679721 1106 80-81 0.36932125004394933 1106 82-83 0.15147359470984867 1106 84-85 0.2566026610490191 1106 86-87 0.15307708157752486 1106 88-89 0.07808139739575637 1106 90-91 0.05098207676425659 1106 92-93 0.30093309557323167 1106 94-95 0.42178811052534826 1106 96-97 0.3303021865294298 1106 98-99 0.32273715147284676 1106 100-101 0.21134578921151714 1107 1 -0.1509359197960798 1107 2 0.05513050865919311 1107 3 -0.052317217151667705 1107 4 -0.022840005735766056 1107 5 -0.04227641904359558 1107 6 -0.046628913684038764 1107 7 -0.13633659969147516 1107 8 0.016147850748886583 1107 9 -0.13181344638737613 1107 10-11 0.003761892627764496 1107 12-13 0.02000765121726289 1107 14-15 0.12666648055105156 1107 16-17 -0.030472536276683115 1107 18-19 -0.11579698329859411 1107 20-21 0.027875148622889867 1107 22-23 -0.06284343344282917 1107 24-25 -0.013759886131452959 1107 26-27 0.030823514446410627 1107 28-29 -0.03795170030149819 1107 30-31 -0.03372901145867502 1107 32-33 0.13562734842060564 1107 34-35 0.0882815409265234 1107 36-37 0.09911920828571397 1107 38-39 -0.043495498810990796 1107 40-41 0.0038696884211120164 1107 42-43 0.025304622834099177 1107 44-45 -0.027246344507688036 1107 46-47 -0.008594664436614607 1107 48-49 -0.12119804761832143 1107 50-51 -0.2533836263344682 1107 52-53 -0.0996481867528658 1107 54-55 0.08356942009962154 1107 56-57 0.20475257171731442 1107 58-59 -0.018642322360456376 1107 60-61 0.024364425175100735 1107 62-63 0.014710836121814452 1107 64-65 0.0024297759486486825 1107 66-67 -0.05370177821858846 1107 68-69 0.006650162213212241 1107 70-71 0.0025966358449522886 1107 72-73 0.05777126470988492 1107 74-75 -0.1327852796267983 1107 76-77 0.07388147167730352 1107 78-79 0.09366889786431898 1107 80-81 0.24147860387646958 1107 82-83 0.20418387109101843 1107 84-85 0.43155439914508165 1107 86-87 0.4562746496581873 1107 88-89 0.36573703035429617 1107 90-91 0.5418757296897425 1107 92-93 0.5279209119456993 1107 94-95 0.36515546972474766 1107 96-97 0.3156874406820904 1107 98-99 0.3353693543784395 1107 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0.153642735226601 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27702.0 3 3993.0 4 3342.0 5 2860.0 6 2853.0 7 3097.0 8 3328.0 9 3300.0 10 3673.0 11 3711.0 12 4144.0 13 4728.0 14 4874.0 15 5548.0 16 6912.0 17 9519.0 18 8161.0 19 5945.0 20 5482.0 21 5575.0 22 5949.0 23 6712.0 24 7731.0 25 8747.0 26 9862.0 27 11726.0 28 13904.0 29 16793.0 30 21036.0 31 26639.0 32 34613.0 33 46893.0 34 66817.0 35 100317.0 36 160298.0 37 268588.0 38 219252.0 39 302.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.29949891651724 21.202897322867383 12.497303302679054 24.000300457936323 2 14.958646429373132 20.65573512751958 42.196451515132985 22.1891669279743 3 18.159077529901495 28.731376525644453 29.436574940214477 23.672971004239578 4 11.92037131173171 17.06180551552344 40.43439070532809 30.583432467416767 5 12.547624163465521 37.67763529105548 35.311930628123626 14.46280991735537 6 27.825204423692014 38.70023040790409 19.979369845736755 13.495195322667142 7 26.06098560081612 31.88171113242253 24.796973778217982 17.260329488543363 8 25.52933552037424 34.675516146895085 21.36828057009798 18.426867762632696 9 26.764262493820564 15.135213978894708 22.27060962891925 35.82991389836548 10-11 24.51119111628175 26.380394890150104 29.148914427657335 19.95949956591081 12-13 24.952911742215317 24.44820597340698 29.003528180242455 21.59535410413525 14-15 22.172271166395973 25.770101017688468 27.141270601717093 24.916357214198463 16-17 21.02327169880868 27.909966834575545 28.260531572663027 22.806229893952747 18-19 20.745460913678095 28.053680313113322 29.95840644813602 21.242452325072563 20-21 21.888008482644295 27.33713794603319 29.057816837070693 21.71703673425182 22-23 21.87307301956633 27.317223995262573 28.663773903291567 22.14592908187953 24-25 21.52166232650072 27.307934009680107 29.618521030407656 21.551882633411516 26-27 21.51086620445339 27.3681443167506 29.051309866314508 22.069679612481504 28-29 21.074811821899406 27.951588137573957 29.130397947116233 21.843202093410405 30-31 22.030288420386995 27.518940088704426 28.788061411829236 21.662710079079346 32-33 22.087279003184484 27.892108310930137 28.208460634137055 21.812152051748324 34-35 21.990722544513794 27.30648094287316 29.250886083467403 21.45191042914564 36-37 22.066011253129023 27.309406896166216 29.06214026059326 21.5624415901115 38-39 22.18278735918304 27.360676585211618 29.175597374852174 21.280938680753167 40-41 21.85081992270236 26.891281088280895 29.356420726248444 21.901478262768304 42-43 21.872898335787642 27.1918447131081 28.650366923277137 22.284890027827124 44-45 21.714034667096392 28.013085647581025 28.600070660051347 21.67280902527123 46-47 21.989337261325037 27.308175132734608 28.343669960756724 22.358817645183628 48-49 22.336819072871396 26.990323046386273 28.450664914800594 22.22219296594174 50-51 21.973205225704223 27.405009610263804 28.03602150419805 22.58576365983392 52-53 21.512049921258807 27.95752575242192 27.913938833754237 22.616485492565037 54-55 21.71628483736976 27.81439867963264 27.882291772977762 22.587024710019836 56-57 22.360308280945336 27.314635813467348 27.799493905010202 22.525562000577118 58-59 22.007486838481245 27.350343143114742 28.393237236621843 22.248932781782173 60-61 22.021553654520755 28.198890403120846 27.77275546973124 22.006800472627155 62-63 22.067740726732783 27.51876190676304 27.653938687174318 22.759558679329857 64-65 21.204612010663304 27.861735982525488 27.932386943142173 23.001265063669035 66-67 21.84243905470786 26.948896905312342 27.87118438133821 23.337479658641584 68-69 21.400242869802096 27.50178870327218 27.818788495410633 23.279179931515092 70-71 21.739495783608152 26.966307379079623 28.144393695632004 23.14980314168022 72-73 22.311582772904966 27.098641992419093 28.301027580335397 22.288747654340543 74-75 22.13311016064747 27.76441706375781 27.713465932080954 22.38900684351376 76-77 22.026882192743567 27.2952648686638 27.97078977422472 22.707063164367916 78-79 21.599744153087578 27.472482218858634 28.119634717199354 22.808138910854435 80-81 21.48949744776311 27.525186288990195 28.443571196177402 22.54174506706929 82-83 22.09921844318225 26.74810455724988 29.04746038911031 22.105216610457564 84-85 22.539209312608488 27.211361452402688 27.821549197426688 22.42788003756214 86-87 21.553632962047235 27.804308634469866 28.252977007223073 22.389081396259826 88-89 22.17471726819669 27.06219793283418 28.022728844465338 22.740355954503794 90-91 22.053275213539028 26.634673931932777 28.661308474543628 22.650742379984564 92-93 22.315636373819572 27.162486074659952 28.225887611894972 22.295989939625503 94-95 21.215339687250115 27.223535505003404 28.518961139855577 23.042163667890907 96-97 21.51321272500374 27.274698923725012 28.234706129791398 22.97738222147985 98-99 21.333742647325707 27.87869962343987 27.818352047641348 22.96920568159307 100-101 21.61169758499863 27.142539173205193 27.73322558597465 23.51253765582153 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 104.0 1 73.5 2 72.5 3 91.5 4 66.0 5 58.0 6 98.5 7 190.5 8 283.0 9 315.5 10 314.0 11 312.0 12 348.0 13 425.0 14 532.5 15 644.5 16 748.0 17 890.0 18 1049.5 19 1213.5 20 1419.0 21 1577.5 22 1759.0 23 2122.5 24 2544.0 25 3084.0 26 4052.5 27 5400.0 28 7388.0 29 10986.0 30 15566.0 31 19907.5 32 25267.0 33 30466.0 34 35204.0 35 39332.0 36 42351.0 37 46997.0 38 46715.5 39 41089.5 40 37032.0 41 33629.5 42 31750.0 43 31893.0 44 33102.5 45 36363.0 46 39541.0 47 41166.0 48 43275.0 49 42953.0 50 40775.5 51 39698.5 52 37446.0 53 35566.0 54 33864.0 55 30678.0 56 27824.5 57 23513.0 58 19436.5 59 17962.5 60 15652.5 61 12505.5 62 9829.0 63 7846.5 64 6305.5 65 4619.0 66 3492.0 67 2912.5 68 2484.0 69 2093.0 70 1643.5 71 1280.0 72 1054.5 73 863.0 74 693.5 75 564.5 76 468.0 77 391.5 78 311.5 79 246.5 80 216.5 81 187.0 82 150.5 83 125.5 84 104.5 85 81.0 86 57.5 87 53.5 88 50.0 89 43.0 90 31.5 91 22.0 92 21.0 93 15.0 94 8.0 95 10.0 96 8.5 97 3.5 98 2.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.113932254136949E-4 2 0.003755701241826983 3 0.0 4 0.0010481026721377627 5 0.0020962053442755255 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 1.3101283401722034E-4 14-15 0.002401901956982373 16-17 0.005153171471343999 18-19 0.0032316499057581014 20-21 0.0 22-23 0.0 24-25 4.3670944672406774E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.734188934481355E-5 42-43 0.0 44-45 6.550641700861016E-4 46-47 0.6802623051620804 48-49 0.4864506527059391 50-51 0.368451760201096 52-53 0.30818585655317465 54-55 0.29918964195065884 56-57 0.2659997239996297 58-59 0.26687314289307784 60-61 0.24451361922080556 62-63 0.2373952552392032 64-65 0.2350370242268933 66-67 0.22263447593992972 68-69 0.2042490082328465 70-71 0.2166078855751376 72-73 0.7440218843837942 74-75 0.4890272384416111 76-77 0.5787273587987346 78-79 0.31604662659420785 80-81 0.2694060576840774 82-83 0.25420856893807986 84-85 0.246173115118357 86-87 0.22232877932722292 88-89 0.2261281515137223 90-91 0.23473132761418644 92-93 0.19433570379221016 94-95 0.166298957312525 96-97 0.1262090301032556 98-99 0.1353362575397886 100-101 0.13192992385534086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1144926.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.86487248670264 #Duplication Level Percentage of deduplicated Percentage of total 1 88.21838996531224 46.636539364984394 2 6.812042754304475 7.202355431605453 3 1.6818907932039995 2.667388269578658 4 0.7367645136690338 1.5579584827136383 5 0.4159314933144752 1.0994082678636772 6 0.28704835047370225 0.9104864667186359 7 0.20877037578945346 0.7725633506577314 8 0.16239200315033592 0.6867866031522177 9 0.12168014308636461 0.5789344723581957 >10 1.0382103240160423 11.284793506716495 >50 0.16550509267803987 6.066509883397693 >100 0.13759781218029765 13.583596602476913 >500 0.009958828063786073 3.5235905266580305 >1k 0.0038175507577846617 3.4290887711182565 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 3432 0.2997573642314001 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 3087 0.26962441240743945 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 2864 0.250147171083546 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 2594 0.22656486096044637 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 2465 0.21529775723496541 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2073 0.18105973661179847 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 1609 0.140533099955805 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1584 0.13834955272218466 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 1534 0.13398245825494398 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 1474 0.12874194489425517 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 1411 0.12323940586553192 No Hit GTACCTGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 1406 0.12280269641880784 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 1396 0.1219292775253597 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 1388 0.12123054241060122 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 1382 0.12070649107453232 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 1362 0.11895965328763607 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 1328 0.1159900290499124 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 1216 0.10620773744329329 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 1161 0.10140393352932853 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.3670944672406774E-5 0.0 18-19 0.0 0.0 0.0 8.734188934481355E-5 0.0 20-21 0.0 0.0 0.0 8.734188934481355E-5 0.0 22-23 0.0 0.0 0.0 1.746837786896271E-4 0.0 24-25 0.0 0.0 0.0 2.1835472336203388E-4 0.0 26-27 0.0 0.0 0.0 4.8038039139647456E-4 0.0 28-29 0.0 0.0 0.0 0.0013974702295170168 0.0 30-31 0.0 0.0 0.0 0.004585449190602711 0.0 32-33 0.0 0.0 0.0 0.014367740797221829 0.0 34-35 0.0 0.0 0.0 0.03187978961085695 0.0 36-37 0.0 0.0 0.0 0.06035324553726616 0.0 38-39 0.0 0.0 0.0 0.09804127078955321 0.0 40-41 0.0 0.0 0.0 0.1410134803472015 0.0 42-43 0.0 0.0 0.0 0.18647493375117694 0.0 44-45 0.0 0.0 0.0 0.2423737429318576 0.0 46-47 0.0 0.0 0.0 0.3085788950552263 0.0 48-49 0.0 0.0 0.0 0.3788454450331288 0.0 50-51 0.0 0.0 0.0 0.44985440107046215 0.0 52-53 0.0 0.0 0.0 0.5254924772430707 0.0 54-55 0.0 0.0 0.0 0.6140134820940393 0.0 56-57 0.0 0.0 0.0 0.7223610958262805 0.0 58-59 0.0 0.0 0.0 0.8397922660503823 0.0 60-61 0.0 0.0 0.0 0.9684031981106203 0.0 62-63 0.0 0.0 0.0 1.0995470449618578 0.0 64-65 0.0 0.0 0.0 1.235276341003698 0.0 66-67 0.0 0.0 0.0 1.3895221175866388 0.0 68-69 0.0 0.0 0.0 1.5697957771943338 0.0 70-71 0.0 0.0 0.0 1.7646118613779405 0.0 72-73 0.0 0.0 0.0 1.9733153059673725 0.0 74-75 0.0 4.3670944672406774E-5 0.0 2.1755554507452883 0.0 76-77 0.0 8.734188934481355E-5 0.0 2.4088456371852853 0.0 78-79 0.0 8.734188934481355E-5 0.0 2.6637529412381236 0.0 80-81 0.0 8.734188934481355E-5 0.0 2.9391855892869936 0.0 82-83 0.0 8.734188934481355E-5 0.0 3.244314479713099 0.0 84-85 0.0 8.734188934481355E-5 0.0 3.5487009640797744 0.0 86-87 0.0 8.734188934481355E-5 0.0 3.8806001435900663 0.0 88-89 0.0 8.734188934481355E-5 0.0 4.237697458176337 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1785 0.0 62.361332 2 ACCTGGG 1900 0.0 59.08544 3 GTACCTG 1925 0.0 58.81024 1 CCTGGGG 1725 0.0 50.800545 4 CTGGGGG 1515 0.0 43.45792 5 ATAACGC 155 0.0 39.728065 3 TATCACG 85 1.6635611E-5 33.43629 2 GTACACG 200 0.0 33.157654 1 TGGGGGG 3270 0.0 30.7069 6 TAACGCA 210 1.8189894E-12 29.323095 4 GTATAAC 300 0.0 28.420847 1 GTAGCGC 90 9.093985E-4 26.3156 3 GTCCTAC 650 0.0 26.234627 1 CTATTGA 1545 0.0 25.139242 9 GCATCGT 720 0.0 24.997637 8 GTACATG 4325 0.0 24.751873 1 GTACTAG 805 0.0 24.713781 1 TAGGCAT 885 0.0 24.619621 5 CATGGGG 2735 0.0 24.5933 4 GAGTACC 425 0.0 24.519945 1 >>END_MODULE