FastQCFastQC Report
Sat 18 Jun 2016
SRR1931067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144926
Sequences flagged as poor quality0
Sequence length101
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA40070.3499789506046679No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA37730.32954094849798155No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC34220.298883945337952No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC31960.27914467834602413No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27990.2444699482761331No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC23820.2080483804193459No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.17250023145600676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.17215086389862752No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC16780.14655969032059712No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC16580.14481285253370085No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA16250.141930570185322No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC13500.11791155061549828No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC13490.11782420872615347No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC13040.11389382370563686No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT12310.10751786578346548No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT12080.10550900232853477No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC11960.104460899656397No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11790.10297608753753519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG17400.059.781572
GTACCTG19100.055.2598271
ACCTGGG18600.055.15863
ATAACGC1250.049.3975943
CCTGGGG16050.048.2375264
CTGGGGG13700.045.7641945
TAACGCA1600.044.5290764
GTACACG1500.041.204251
GTACCCG1550.039.875081
TGGGGGG24350.030.819856
GTATAAC3050.029.6171781
GTACTAG5800.028.6899621
ACCAGTA6750.028.1467742
GGGCTAT16850.027.906656
CCAGTAC8600.027.6149353
TATCACG1056.9081056E-527.1415352
GCTATTG16150.027.0575078
CTATTGA16100.026.8465189
TGGGGGA10000.025.648756
AGGGCTA17350.025.4598545
Produced by FastQC (version 0.11.5)