##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931066_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3917366
Sequences flagged as poor quality	0
Sequence length	101
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.622513188708943	33.0	33.0	33.0	27.0	33.0
2	31.562562956843962	33.0	33.0	33.0	27.0	33.0
3	31.581136151179134	33.0	33.0	33.0	27.0	33.0
4	35.269872919711865	37.0	37.0	37.0	33.0	37.0
5	35.27378728461931	37.0	37.0	37.0	33.0	37.0
6	35.364811202221084	37.0	37.0	37.0	33.0	37.0
7	35.33929456680841	37.0	37.0	37.0	33.0	37.0
8	35.33892084630336	37.0	37.0	37.0	33.0	37.0
9	35.34987565624453	37.0	37.0	37.0	33.0	37.0
10-11	35.27915530486557	37.0	37.0	37.0	33.0	37.0
12-13	35.218473714225325	37.0	37.0	37.0	33.0	37.0
14-15	37.01907544508223	40.0	37.0	40.0	33.0	40.0
16-17	36.98035848577845	40.0	37.0	40.0	33.0	40.0
18-19	36.93121717500995	40.0	37.0	40.0	33.0	40.0
20-21	36.86127489746937	40.0	37.0	40.0	33.0	40.0
22-23	36.796055691502914	40.0	37.0	40.0	33.0	40.0
24-25	36.699628015355216	40.0	37.0	40.0	33.0	40.0
26-27	36.558358345888536	40.0	37.0	40.0	33.0	40.0
28-29	36.47427723118034	40.0	37.0	40.0	33.0	40.0
30-31	36.378920172381136	40.0	37.0	40.0	33.0	40.0
32-33	36.25477450919827	40.0	37.0	40.0	33.0	40.0
34-35	36.18301978931762	40.0	37.0	40.0	33.0	40.0
36-37	36.01847057436042	40.0	37.0	40.0	33.0	40.0
38-39	35.89699213706353	40.0	37.0	40.0	33.0	40.0
40-41	35.711640551329644	37.0	37.0	40.0	27.0	40.0
42-43	35.59411374888126	37.0	37.0	40.0	27.0	40.0
44-45	35.41802846606623	37.0	37.0	40.0	27.0	40.0
46-47	35.33618329254912	37.0	37.0	40.0	27.0	40.0
48-49	35.393439750076965	40.0	37.0	40.0	27.0	40.0
50-51	34.6155915735216	38.5	35.0	38.5	27.0	40.0
52-53	34.736632599557964	37.0	37.0	38.5	27.0	40.0
54-55	35.30830524898618	37.0	37.0	40.0	27.0	40.0
56-57	35.311979784375524	37.0	37.0	40.0	27.0	40.0
58-59	35.21490958465459	37.0	37.0	40.0	27.0	40.0
60-61	35.26496860390374	37.0	37.0	40.0	27.0	40.0
62-63	35.1295616748601	37.0	37.0	40.0	27.0	40.0
64-65	34.943091735620314	37.0	37.0	40.0	27.0	40.0
66-67	34.71449604147277	37.0	37.0	40.0	27.0	40.0
68-69	34.501366989962136	37.0	33.0	40.0	27.0	40.0
70-71	34.24442916490315	37.0	33.0	40.0	27.0	40.0
72-73	33.60750757013769	37.0	33.0	40.0	22.0	40.0
74-75	33.39740453151429	37.0	33.0	37.0	22.0	40.0
76-77	32.99657869599113	37.0	33.0	37.0	22.0	40.0
78-79	32.692115186582	37.0	33.0	37.0	22.0	38.5
80-81	32.38573572140055	37.0	33.0	37.0	22.0	37.0
82-83	32.08924504373602	37.0	33.0	37.0	18.5	37.0
84-85	31.683480430472926	37.0	33.0	37.0	15.0	37.0
86-87	31.491091080077787	37.0	33.0	37.0	15.0	37.0
88-89	31.253608674808532	37.0	33.0	37.0	10.5	37.0
90-91	30.931518270184608	37.0	33.0	37.0	4.0	37.0
92-93	30.63036808406465	35.0	33.0	37.0	2.0	37.0
94-95	30.34439021015652	33.0	33.0	37.0	2.0	37.0
96-97	30.01497549629011	33.0	33.0	37.0	2.0	37.0
98-99	29.654498711634297	33.0	33.0	37.0	2.0	37.0
100-101	28.907635768524052	33.0	30.0	37.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.08960639936269743
1101	2	0.07773025172484438
1101	3	0.13813274804744324
1101	4	0.0775413899587889
1101	5	0.04605363495575432
1101	6	0.029278308445974233
1101	7	0.13371076938078374
1101	8	0.16262883493107694
1101	9	0.13277153880090964
1101	10-11	0.15653869481160854
1101	12-13	0.19225804916960954
1101	14-15	0.3235454839479672
1101	16-17	0.22834776323539074
1101	18-19	0.28293125918047934
1101	20-21	0.35716296823446214
1101	22-23	0.30776530493570675
1101	24-25	0.255994261418671
1101	26-27	0.3161046153903655
1101	28-29	0.24224371795197186
1101	30-31	0.30030511090959777
1101	32-33	0.2836207858300881
1101	34-35	0.27895157612523036
1101	36-37	0.29113594764091744
1101	38-39	0.27865834231994313
1101	40-41	0.26895352006224726
1101	42-43	0.33026254414775735
1101	44-45	0.2886860972009657
1101	46-47	0.32534294061293423
1101	48-49	0.2571044677762586
1101	50-51	0.2975879751150572
1101	52-53	0.42075899395164384
1101	54-55	0.5098282534817287
1101	56-57	0.5199807105891807
1101	58-59	0.5339677029343122
1101	60-61	0.4453541153066993
1101	62-63	0.48053079589286796
1101	64-65	0.4926142242494578
1101	66-67	0.45151317650820744
1101	68-69	0.4478369075202906
1101	70-71	0.343987301006905
1101	72-73	0.27912021617706273
1101	74-75	0.2719052073399624
1101	76-77	0.12016444356437717
1101	78-79	0.3197532600726376
1101	80-81	0.41152983614078664
1101	82-83	0.29550558328134713
1101	84-85	0.3781051596623506
1101	86-87	0.28207448891669173
1101	88-89	0.18575268196540407
1101	90-91	0.10460452248508645
1101	92-93	0.05240365975709338
1101	94-95	0.0717786064806063
1101	96-97	0.021270237605062192
1101	98-99	0.00930725780119701
1101	100-101	0.14959527143983564
1102	1	0.11190471887939424
1102	2	0.02798935578020334
1102	3	0.06931449148018487
1102	4	0.21367225070364526
1102	5	0.05484456000839799
1102	6	0.03643032355703468
1102	7	0.13205398646747568
1102	8	0.06498139185088547
1102	9	0.1464159316714415
1102	10-11	0.22150295676553355
1102	12-13	0.17613072644184768
1102	14-15	0.2768661864937485
1102	16-17	0.2945423091193504
1102	18-19	0.273994440800557
1102	20-21	0.2787661289073995
1102	22-23	0.34156396732250727
1102	24-25	0.36943496891569083
1102	26-27	0.271828858546904
1102	28-29	0.24107611411810836
1102	30-31	0.3489364278308855
1102	32-33	0.31437663712929265
1102	34-35	0.3284342699761069
1102	36-37	0.3265711507614242
1102	38-39	0.4324182710674762
1102	40-41	0.41772963299349186
1102	42-43	0.44803445460301106
1102	44-45	0.49123919717646913
1102	46-47	0.36615383484592456
1102	48-49	0.37887643243233526
1102	50-51	0.27092653068133643
1102	52-53	0.39467704945212034
1102	54-55	0.37180003131121
1102	56-57	0.41954953138122164
1102	58-59	0.4720597191443474
1102	60-61	0.4687100159685542
1102	62-63	0.5260456068920192
1102	64-65	0.49025033669688156
1102	66-67	0.49532656372979034
1102	68-69	0.5945656091935447
1102	70-71	0.5734430474042327
1102	72-73	0.3343694373103645
1102	74-75	0.37545276777492376
1102	76-77	0.3780625721146009
1102	78-79	0.38622246781034875
1102	80-81	0.4438433792728844
1102	82-83	0.36581839436976793
1102	84-85	0.3138822103842607
1102	86-87	0.2730814745884693
1102	88-89	0.3390402654789426
1102	90-91	0.3272006223366404
1102	92-93	0.29082835431839626
1102	94-95	0.1865418307547273
1102	96-97	0.20522339312809734
1102	98-99	0.2348055957395232
1102	100-101	0.2654910602102447
1103	1	0.04918697985866416
1103	2	0.03396313187989719
1103	3	0.039804698099334956
1103	4	0.054702533248757845
1103	5	0.02057434425864102
1103	6	0.019411983410883238
1103	7	0.07108342997145911
1103	8	0.09235376191252698
1103	9	0.02114582577939217
1103	10-11	0.030808255537486673
1103	12-13	0.08092766654176131
1103	14-15	0.2301218511340295
1103	16-17	0.11826812959124311
1103	18-19	0.11361238428778364
1103	20-21	0.14683815433442504
1103	22-23	0.07750329401424239
1103	24-25	0.13343052401213384
1103	26-27	0.13864970077836603
1103	28-29	0.12003470994459775
1103	30-31	0.23987326105729068
1103	32-33	0.18849423265572085
1103	34-35	0.14415874873176193
1103	36-37	0.16942263509358213
1103	38-39	0.1301430454316943
1103	40-41	0.2283258183311716
1103	42-43	0.11295792079388889
1103	44-45	0.17787958700499473
1103	46-47	-4.5958948425095514E-4
1103	48-49	0.09833218472452643
1103	50-51	0.11253116522411233
1103	52-53	0.24031362564898728
1103	54-55	0.28310082849398555
1103	56-57	0.3022079191339344
1103	58-59	0.17038379187155073
1103	60-61	0.1805064142755768
1103	62-63	0.20177471419395232
1103	64-65	0.28656244406978004
1103	66-67	0.19696241285136296
1103	68-69	0.20030915259400928
1103	70-71	0.32745525449232815
1103	72-73	0.2742256244423018
1103	74-75	0.3329472063751311
1103	76-77	0.1913481630237186
1103	78-79	0.31623690390888726
1103	80-81	0.348008344784823
1103	82-83	0.3512165723247236
1103	84-85	0.3599178581546987
1103	86-87	0.36274398188792034
1103	88-89	0.35131305738011065
1103	90-91	0.33168862571154634
1103	92-93	0.20573267153872266
1103	94-95	0.23474921773657087
1103	96-97	0.27072868351433144
1103	98-99	0.27237903819036546
1103	100-101	0.3835839913941115
1104	1	-0.03366306156635801
1104	2	-0.031592614734453406
1104	3	-0.07603946045167476
1104	4	-0.06793637083799098
1104	5	-0.036644291268913776
1104	6	-0.12802461546465338
1104	7	0.013692841527735311
1104	8	-0.07069407747302847
1104	9	-0.1515678517724126
1104	10-11	-0.0886859944612155
1104	12-13	-0.10346838792041524
1104	14-15	-0.09571850361005829
1104	16-17	-0.016931153417196754
1104	18-19	0.029866103420935985
1104	20-21	0.0052010583586437065
1104	22-23	0.021261663692172306
1104	24-25	-0.03766262757218186
1104	26-27	0.02879002477934023
1104	28-29	-0.0016245835894537208
1104	30-31	-0.033380765506827004
1104	32-33	-0.0698702345768325
1104	34-35	-0.06402152045271237
1104	36-37	-0.17738294868656368
1104	38-39	-0.08508208262111339
1104	40-41	-0.00464693494765811
1104	42-43	-0.0430268222928234
1104	44-45	-0.12198285066206438
1104	46-47	-0.22411301577637488
1104	48-49	-0.24922634440064684
1104	50-51	-0.2755353023940188
1104	52-53	-0.21739377301603469
1104	54-55	-0.08632177445630873
1104	56-57	-0.06532841243297582
1104	58-59	-0.16553404589868848
1104	60-61	-0.1551977850230628
1104	62-63	-0.12145508264107718
1104	64-65	-0.11097706602073032
1104	66-67	-0.15503823327474464
1104	68-69	-0.12424155527882164
1104	70-71	-0.05862333320105506
1104	72-73	-0.1902608876751657
1104	74-75	-0.15285625665239166
1104	76-77	-0.11805245079607829
1104	78-79	0.01590810909039675
1104	80-81	-0.05649265306316664
1104	82-83	-0.0636589181318854
1104	84-85	0.05242696384321377
1104	86-87	0.005444059527263789
1104	88-89	-0.01702572112890266
1104	90-91	0.13296837226336677
1104	92-93	0.1411070338799938
1104	94-95	0.05859908720867324
1104	96-97	0.07497331519639161
1104	98-99	0.13487923359140908
1104	100-101	0.21581526285566
1105	1	-0.08256931100018505
1105	2	-0.08393174526494818
1105	3	-0.03125038485124065
1105	4	-0.02626144591773283
1105	5	-0.0977714798143623
1105	6	-0.10980864573077298
1105	7	5.747125286106325E-4
1105	8	0.013651480172228503
1105	9	-0.004003257758185441
1105	10-11	-0.07356769878153102
1105	12-13	-0.07351583034811426
1105	14-15	-0.11720511778715093
1105	16-17	-0.0668212146685434
1105	18-19	-0.05351116188440841
1105	20-21	0.040438374074248884
1105	22-23	-0.04644274098853174
1105	24-25	-0.03918360474411742
1105	26-27	0.038570850786108224
1105	28-29	-0.0020965603012115253
1105	30-31	-0.030531163807040684
1105	32-33	-0.03214187178804906
1105	34-35	0.002645677203425123
1105	36-37	-0.019748606584911954
1105	38-39	-0.16480601504343184
1105	40-41	-0.04864461974242573
1105	42-43	-0.06113299343191869
1105	44-45	-0.20676606441223555
1105	46-47	-0.09549954983332043
1105	48-49	-0.059176394244147446
1105	50-51	-0.12096698008168971
1105	52-53	-0.0071216358998782425
1105	54-55	0.02339529179360511
1105	56-57	-0.022794583561122295
1105	58-59	0.02155318812771867
1105	60-61	0.008173567839875773
1105	62-63	0.05950660674225361
1105	64-65	0.01345390071077901
1105	66-67	0.0271242329575756
1105	68-69	-0.00506417099994394
1105	70-71	0.027561692745528887
1105	72-73	0.04335585957769439
1105	74-75	0.014814059871802954
1105	76-77	0.064074840232621
1105	78-79	0.023563040137773328
1105	80-81	0.13664560476677678
1105	82-83	0.1299754160391089
1105	84-85	0.10574727403313133
1105	86-87	0.0866136962368067
1105	88-89	0.04067367971671132
1105	90-91	0.12062642224715958
1105	92-93	0.15853752661793763
1105	94-95	0.02394744376094593
1105	96-97	0.0822222102198431
1105	98-99	0.2073780971486059
1105	100-101	0.21741896499627344
1106	1	0.12936769923398828
1106	2	0.046317458246335264
1106	3	0.1251195500364659
1106	4	0.10129043574880825
1106	5	0.053931722398949944
1106	6	0.11865126147426253
1106	7	0.15629613503837447
1106	8	0.16538572495321802
1106	9	0.1749134553708842
1106	10-11	0.10229581888379613
1106	12-13	0.11551788553245501
1106	14-15	0.22472886664205305
1106	16-17	0.18803596652563925
1106	18-19	0.11547084749356173
1106	20-21	0.1938949741299254
1106	22-23	0.13817308999057332
1106	24-25	0.22594487902325966
1106	26-27	0.28160696810347474
1106	28-29	0.32896393632035625
1106	30-31	0.37855114079317786
1106	32-33	0.3462604003926444
1106	34-35	0.2823945002234538
1106	36-37	0.3489151589881132
1106	38-39	0.26279480431804814
1106	40-41	0.2777080584534133
1106	42-43	0.2890121897489948
1106	44-45	0.25624137335992714
1106	46-47	0.3580876860039055
1106	48-49	0.27551987012436996
1106	50-51	0.21225787334194024
1106	52-53	0.30976130075136865
1106	54-55	0.34727694102367934
1106	56-57	0.3331220486814317
1106	58-59	0.4296109865482407
1106	60-61	0.30525344286886025
1106	62-63	0.31746388540603476
1106	64-65	0.3671628585813451
1106	66-67	0.37801098858858495
1106	68-69	0.4684377173891079
1106	70-71	0.4270253664487811
1106	72-73	0.17418104136270784
1106	74-75	0.2856297922721538
1106	76-77	0.19331563371039806
1106	78-79	0.301483868469802
1106	80-81	0.3781450473531791
1106	82-83	0.38439644577585597
1106	84-85	0.44657488511886
1106	86-87	0.5243296585009425
1106	88-89	0.4175632523085717
1106	90-91	0.45128377127410246
1106	92-93	0.5848543739807504
1106	94-95	0.5964744805524091
1106	96-97	0.5530041542393427
1106	98-99	0.5336804241644053
1106	100-101	0.48534601401539845
1107	1	-0.06265452861683585
1107	2	-0.01322954183809344
1107	3	-0.012396472283487725
1107	4	-0.002335789430986779
1107	5	-0.0783554589775477
1107	6	-0.1278038721921817
1107	7	-0.0983501458645506
1107	8	-0.09092726099319037
1107	9	0.0010545899430169925
1107	10-11	0.023792207625575656
1107	12-13	0.0015001784703514431
1107	14-15	0.014023846187214417
1107	16-17	0.02380315741958583
1107	18-19	-0.03474291136188157
1107	20-21	0.026999197882098258
1107	22-23	-0.004163060200930602
1107	24-25	0.051579823415259796
1107	26-27	0.025450943327406605
1107	28-29	-0.07771574008634019
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2316	64-65	-0.7052969178830111
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2316	72-73	-0.43170906163292244
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2316	76-77	-0.6761811886988127
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2316	80-81	-0.5123902863080829
2316	82-83	-0.5225866765542762
2316	84-85	-0.5450372915683239
2316	86-87	-0.6992710196885277
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2316	90-91	-0.6167398044995558
2316	92-93	-0.5987043156322223
2316	94-95	-0.5822648716607866
2316	96-97	-0.6501320712265901
2316	98-99	-0.5115249878000121
2316	100-101	-0.6391666924685211
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	74942.0
3	11354.0
4	8747.0
5	7455.0
6	7495.0
7	7879.0
8	8162.0
9	8454.0
10	8550.0
11	8601.0
12	8574.0
13	8936.0
14	9661.0
15	10603.0
16	12380.0
17	15024.0
18	13877.0
19	11803.0
20	12423.0
21	13770.0
22	15439.0
23	17875.0
24	21273.0
25	24891.0
26	28400.0
27	34308.0
28	41842.0
29	51115.0
30	64255.0
31	81624.0
32	106597.0
33	142050.0
34	199212.0
35	294113.0
36	473329.0
37	984323.0
38	1067647.0
39	383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.805986090589855	23.66335592344617	13.56295126192516	22.967706724038813
2	13.693516789687395	22.153023593736997	43.769672722482696	20.38378689409291
3	17.06136275450814	30.517516899136155	31.232539261135052	21.188581085220658
4	10.764045511739024	19.016092383591943	43.751427953659125	26.468434151009912
5	11.564394014817447	39.23417151825478	37.13865815870935	12.06277630821842
6	25.19266772622216	42.11579923857	21.14813882593559	11.54339420927225
7	23.575764940013265	32.9879822309174	27.818054274224057	15.618198554845272
8	22.179878009866837	38.6496691909819	22.788756526706976	16.38169627244429
9	25.455420810820335	16.351471881871646	23.89146176282737	34.30164554448065
10-11	22.11815421264838	28.060735236849986	31.900103219933506	17.921007330568123
12-13	22.658877923746154	25.824591695995498	31.045080580992877	20.471449799265475
14-15	20.376917188730342	27.818253552609224	27.968244879380862	23.836584379279575
16-17	19.5205647748637	29.72744491784442	30.06790741738012	20.684082889911764
18-19	19.199723886028444	30.579903160989304	30.68823245447094	19.532140498511318
20-21	19.735786750587	29.599136256351844	30.09806844701261	20.567008546048545
22-23	19.916507673778757	30.071098794445046	29.400380255508416	20.612013276267778
24-25	19.843129286362316	29.86539935252412	30.05714809389779	20.234323267215775
26-27	20.14183765315776	29.484352496039428	29.5009452780261	20.87286457277671
28-29	19.89475836569777	30.099306523822385	29.6160226029429	20.389912507536952
30-31	20.870541583298575	29.86649702887093	29.123255268974102	20.139706118856395
32-33	20.597769011116142	29.955689613888513	29.170046914176517	20.276494460818824
34-35	20.923268338980836	29.205938377981532	29.675143961529255	20.19564932150838
36-37	21.17273443431122	29.545720262033214	29.485233189852565	19.796312113803
38-39	21.599883697361953	29.007348304957976	30.028174543813368	19.364593453866703
40-41	20.810593172150536	28.783016391040533	30.405833938652716	20.000556498156215
42-43	21.210632502890018	29.01083649202506	29.50380810777039	20.274722897314533
44-45	20.46441445046341	29.823414680593967	29.601580561715135	20.11059030722749
46-47	21.148333147324585	29.040495733027903	28.972105373869866	20.839065745777642
48-49	21.181527372370716	28.10349559581334	29.742505620311622	20.972471411504326
50-51	20.91122233407171	28.817649806584395	28.932218996314944	21.338908863028948
52-53	20.063628314945944	28.973475053466508	29.66856848073919	21.29432815084836
54-55	20.878667324719736	29.26710235790113	28.98379719330292	20.87043312407621
56-57	20.964220841541906	28.499585288103475	29.492154270521198	21.044039599833425
58-59	20.302176538528208	28.338898281151863	30.513196492201004	20.84572868811892
60-61	20.531904572847562	29.678508412551775	28.806855982038616	20.98273103256205
62-63	20.978226509317693	28.83448538003463	28.39404988462469	21.793238226022986
64-65	19.99786278298929	28.81246240673671	28.825477674400744	22.36419713587325
66-67	20.571869853574395	27.89817898908366	29.20578536614079	22.324165791201157
68-69	19.891142105941217	28.47284866273333	28.925728104227648	22.710281127097808
70-71	19.855694981312514	28.27257500905265	29.541903858924506	22.329826150710332
72-73	20.173318797881034	28.207810643821713	29.546189179117537	22.072681379179716
74-75	20.28490570299034	28.904553727891198	28.774600837899072	22.03593973121939
76-77	20.005404929120672	28.71441808513945	29.458816058496538	21.821360927243337
78-79	19.952522509090482	28.4080784232954	29.102193038410835	22.53720602920328
80-81	20.049690306178167	28.62997403972126	29.602348242089555	21.717987412011016
82-83	20.642325376588534	27.716429602777183	29.650164357469816	21.99108066316447
84-85	21.064858930843073	27.580779120180228	29.22369438089959	22.13066756807712
86-87	20.29056181460315	28.613148717376514	29.102630608478723	21.993658859541615
88-89	21.08767620804748	27.314725062276885	29.405618669540228	22.191980060135407
90-91	20.972745241951326	26.99886675800004	29.569297652314187	22.459090347734445
92-93	20.268805230633006	27.90882772935476	29.48421155910846	22.338155480903783
94-95	20.044223699642025	27.053504896359737	29.681082187767473	23.22118921623077
96-97	20.39088131917492	27.115999822578836	29.27010748680238	23.223011371443857
98-99	20.03294609863836	27.772377383421155	29.47355274047571	22.721123777464776
100-101	20.651390673721373	26.525748948946188	29.019222246382174	23.803638130950265
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	31.0
1	20.0
2	17.5
3	28.5
4	27.0
5	24.5
6	37.5
7	58.5
8	88.5
9	103.0
10	97.0
11	110.0
12	160.5
13	274.0
14	418.5
15	581.0
16	786.5
17	953.0
18	1031.5
19	1071.5
20	1101.5
21	1180.5
22	1293.0
23	1404.5
24	1589.0
25	1837.0
26	2237.0
27	3030.5
28	5175.0
29	10699.5
30	19973.0
31	34031.5
32	55890.0
33	88938.0
34	137959.0
35	184860.5
36	233127.5
37	295831.0
38	329463.5
39	309967.5
40	274646.5
41	238655.5
42	204984.0
43	180395.0
44	157699.5
45	134514.0
46	111571.0
47	95844.5
48	86241.0
49	77219.0
50	68788.0
51	64362.0
52	60740.0
53	57406.5
54	54427.0
55	49732.0
56	44987.0
57	38733.0
58	32763.5
59	29812.0
60	25641.5
61	20671.5
62	16531.5
63	13468.5
64	10765.0
65	7829.0
66	5995.5
67	4763.0
68	3856.0
69	3155.5
70	2638.0
71	2203.5
72	1862.0
73	1564.0
74	1295.5
75	1081.5
76	898.0
77	723.0
78	569.5
79	468.5
80	403.0
81	348.5
82	293.0
83	240.5
84	199.0
85	167.0
86	148.0
87	137.0
88	102.5
89	72.0
90	64.0
91	50.5
92	41.0
93	30.0
94	24.0
95	18.0
96	12.5
97	10.0
98	6.0
99	3.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	4.59492424246292E-4
2	0.003573829966360049
3	1.5316414141543068E-4
4	0.0011997857744208737
5	0.002348516835036604
6	0.0
7	0.0
8	0.0
9	0.0
10-11	6.381839225642945E-5
12-13	7.658207070771534E-5
14-15	0.003561066287908763
16-17	0.004965070917550211
18-19	0.0036504120370677647
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	3.190919612821472E-4
42-43	1.2763678451285889E-5
44-45	6.254202441130086E-4
46-47	0.7316140488277072
48-49	0.5249062763091322
50-51	0.4031663112407674
52-53	0.3391692274860199
54-55	0.3297879238243248
56-57	0.29720225273819195
58-59	0.29787872769611007
60-61	0.27475094234238007
62-63	0.26933914267903486
64-65	0.2658929494971876
66-67	0.25325690783041466
68-69	0.23361360669388564
70-71	0.24687506860477168
72-73	0.80906404967011
74-75	0.5349002365364891
76-77	0.6285601090120249
78-79	0.3530944006763728
80-81	0.3057283899436509
82-83	0.28708065572632224
84-85	0.2781333171319708
86-87	0.2518784305576757
88-89	0.2557202977715128
90-91	0.26633967824298266
92-93	0.2220752413739232
94-95	0.19072764709756504
96-97	0.14728008564938788
98-99	0.15645717045586244
100-101	0.15149209953831222
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3917366.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.894786142424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.91158219834058	32.22194288074944
2	12.577144795102294	10.538335829462236
3	4.431028156386803	5.569109310086532
4	2.0771848758683866	3.480928646111344
5	1.083481018879299	2.2696102787661947
6	0.6450792119119084	1.621527337678369
7	0.41353879018595524	1.212758342350614
8	0.28425078197366577	0.9526900577282815
9	0.2126368975507287	0.8017539613988671
>10	1.0750419028265172	8.572913114973629
>50	0.13404848824632692	3.9493985355107966
>100	0.12860489423121693	10.912164409413826
>500	0.014837618531291593	4.240540576335511
>1k	0.010074926159956898	8.112381868367699
>5k	9.159023781778997E-4	2.538558382623057
>10k+	5.495414269067398E-4	3.0053864684436076
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	17119	0.4370028228151263	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	15964	0.4075187255926559	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	14403	0.3676705214677413	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	12450	0.31781559343701865	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	12338	0.3149565294639306	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	12236	0.31235273905986827	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	11392	0.2908076498340977	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10983	0.2803669608609458	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	10600	0.27058998316726085	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9130	0.23306476852048033	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	8795	0.22451310395811877	No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA	8402	0.2144808526954081	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	7449	0.1901532815672572	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7117	0.18167819907560337	No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG	7050	0.17996786616313104	No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA	6267	0.15997994570841734	No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA	6164	0.15735062794745244	No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG	5864	0.14969242087668092	No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA	5861	0.1496158388059732	No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG	5800	0.1480586700349163	No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA	5559	0.14190657702139653	No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT	5356	0.13672452357017445	No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG	5275	0.13465680766106614	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5147	0.13138930597753695	No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT	4918	0.125543541246848	No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC	4844	0.12365451683605769	No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT	4738	0.12094861700438508	No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT	4685	0.11959566708854878	No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG	4637	0.11837035395722534	No Hit
GTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAA	4589	0.1171450408259019	No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA	4380	0.11180982323326438	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4165	0.10632144149921145	No Hit
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG	3936	0.10047567676852251	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	1.2763678451285889E-5
12-13	0.0	0.0	0.0	0.0	2.5527356902571778E-5
14-15	0.0	0.0	0.0	2.5527356902571778E-5	2.5527356902571778E-5
16-17	0.0	0.0	0.0	2.5527356902571778E-5	2.5527356902571778E-5
18-19	0.0	0.0	0.0	5.1054713805143556E-5	3.829103535385767E-5
20-21	0.0	0.0	0.0	1.276367845128589E-4	5.1054713805143556E-5
22-23	0.0	0.0	0.0	4.722561026975779E-4	5.1054713805143556E-5
24-25	0.0	0.0	0.0	0.0010593853114567288	5.1054713805143556E-5
26-27	0.0	0.0	0.0	0.002437862584195605	7.658207070771534E-5
28-29	0.0	0.0	0.0	0.006586058080863519	7.658207070771534E-5
30-31	0.0	0.0	0.0	0.026076195075977072	7.658207070771534E-5
32-33	0.0	0.0	0.0	0.0860144290832156	7.658207070771534E-5
34-35	0.0	0.0	0.0	0.1875877821985487	7.658207070771534E-5
36-37	0.0	0.0	0.0	0.3517542144389878	7.658207070771534E-5
38-39	0.0	0.0	0.0	0.5764715372523272	7.658207070771534E-5
40-41	0.0	0.0	0.0	0.8023631184831849	7.658207070771534E-5
42-43	0.0	0.0	0.0	1.0463280684010634	7.658207070771534E-5
44-45	0.0	0.0	0.0	1.3341362537991088	7.658207070771534E-5
46-47	0.0	0.0	0.0	1.6812189619249263	7.658207070771534E-5
48-49	0.0	0.0	0.0	2.0234387085608034	7.658207070771534E-5
50-51	1.2763678451285889E-5	0.0	0.0	2.363360993075449	7.658207070771534E-5
52-53	2.5527356902571778E-5	0.0	0.0	2.7005390867230687	8.934574915900122E-5
54-55	2.5527356902571778E-5	0.0	0.0	3.056211750446601	1.0210942761028711E-4
56-57	2.5527356902571778E-5	0.0	0.0	3.47676219173802	1.0210942761028711E-4
58-59	2.5527356902571778E-5	0.0	0.0	3.9577103594609233	1.0210942761028711E-4
60-61	2.5527356902571778E-5	0.0	0.0	4.433706219944728	1.0210942761028711E-4
62-63	2.5527356902571778E-5	0.0	0.0	4.9015077988628075	1.0210942761028711E-4
64-65	2.5527356902571778E-5	0.0	0.0	5.39971245985185	1.0210942761028711E-4
66-67	2.5527356902571778E-5	0.0	0.0	5.926443942179516	1.14873106061573E-4
68-69	2.5527356902571778E-5	0.0	0.0	6.484969747529335	1.276367845128589E-4
70-71	2.5527356902571778E-5	0.0	0.0	7.05110270523612	1.276367845128589E-4
72-73	2.5527356902571778E-5	0.0	0.0	7.65499062380181	1.276367845128589E-4
74-75	2.5527356902571778E-5	0.0	0.0	8.239809606761277	1.4040046296414477E-4
76-77	2.5527356902571778E-5	0.0	0.0	8.877789310470352	1.5316414141543068E-4
78-79	2.5527356902571778E-5	0.0	0.0	9.58478222356553	1.5316414141543068E-4
80-81	2.5527356902571778E-5	0.0	0.0	10.34602077007867	1.5316414141543068E-4
82-83	2.5527356902571778E-5	0.0	0.0	11.140572517349668	1.7869149831800245E-4
84-85	2.5527356902571778E-5	0.0	0.0	11.888204982633738	1.7869149831800245E-4
86-87	2.5527356902571778E-5	0.0	0.0	12.70204264804463	1.7869149831800245E-4
88-89	2.5527356902571778E-5	0.0	0.0	13.540258939297477	1.7869149831800245E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTGGG	4045	0.0	52.463974	3
TACCTGG	4320	0.0	49.344814	2
CCTGGGG	3485	0.0	44.718708	4
GTACCTG	5150	0.0	41.85207	1
CTGGGGG	3150	0.0	39.69991	5
GTACCCG	445	0.0	31.93552	1
TATTCGC	995	0.0	31.898483	9
TGGGGGG	6495	0.0	31.360277	6
GTACACG	550	0.0	30.145197	1
GGGGGGA	6650	0.0	26.284233	7
GTTATAA	5630	0.0	25.157967	1
GTACATG	9505	0.0	25.068514	1
GAACCGT	685	0.0	24.202934	6
GTACTAG	1645	0.0	24.189459	1
CATGGGG	5970	0.0	24.041658	4
ACATGGG	9165	0.0	23.982098	3
AACCGTG	700	0.0	23.684301	7
ACCGTGT	725	0.0	22.867891	8
GGGGAAT	7550	0.0	22.83877	9
TACATGG	10125	0.0	22.831663	2
>>END_MODULE