##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1931065_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3639764
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.456859016134015	33.0	33.0	33.0	27.0	33.0
2	31.388827407491256	33.0	33.0	33.0	27.0	33.0
3	31.404635025787385	33.0	33.0	33.0	27.0	33.0
4	35.07286131738211	37.0	37.0	37.0	33.0	37.0
5	35.062144413758695	37.0	37.0	37.0	33.0	37.0
6	35.152359603534734	37.0	37.0	37.0	33.0	37.0
7	35.13105272759443	37.0	37.0	37.0	33.0	37.0
8	35.13123433277542	37.0	37.0	37.0	33.0	37.0
9	35.145140454161314	37.0	37.0	37.0	33.0	37.0
10-11	35.06354079550213	37.0	37.0	37.0	33.0	37.0
12-13	34.99051902815677	37.0	37.0	37.0	33.0	37.0
14-15	36.75929098699805	40.0	37.0	40.0	33.0	40.0
16-17	36.71785217393216	40.0	37.0	40.0	33.0	40.0
18-19	36.65712254970377	40.0	37.0	40.0	33.0	40.0
20-21	36.582099278964236	40.0	37.0	40.0	33.0	40.0
22-23	36.50342879373498	40.0	37.0	40.0	33.0	40.0
24-25	36.40076952791445	40.0	37.0	40.0	33.0	40.0
26-27	36.24426817233205	40.0	37.0	40.0	33.0	40.0
28-29	36.14937616834498	40.0	37.0	40.0	33.0	40.0
30-31	36.03686035688028	40.0	37.0	40.0	33.0	40.0
32-33	35.89715445836598	40.0	37.0	40.0	33.0	40.0
34-35	35.81233906923636	40.0	37.0	40.0	30.0	40.0
36-37	35.63408685288387	40.0	37.0	40.0	27.0	40.0
38-39	35.4938324297949	38.5	37.0	40.0	27.0	40.0
40-41	35.29130199100821	37.0	37.0	40.0	27.0	40.0
42-43	35.150448078501796	37.0	37.0	40.0	27.0	40.0
44-45	34.943445096989805	37.0	37.0	40.0	27.0	40.0
46-47	34.828900033079066	37.0	37.0	40.0	27.0	40.0
48-49	34.85276270659307	37.0	37.0	40.0	27.0	40.0
50-51	34.077251162438	37.0	35.0	38.5	22.0	40.0
52-53	34.20445460200167	37.0	35.0	38.5	24.5	40.0
54-55	34.74145205568273	37.0	37.0	40.0	27.0	40.0
56-57	34.728706723842535	37.0	37.0	40.0	27.0	40.0
58-59	34.60803694964839	37.0	37.0	40.0	27.0	40.0
60-61	34.64801069519892	37.0	37.0	40.0	27.0	40.0
62-63	34.501935152938486	37.0	37.0	40.0	27.0	40.0
64-65	34.308354744977976	37.0	33.0	40.0	22.0	40.0
66-67	34.09821144997313	37.0	33.0	40.0	22.0	40.0
68-69	33.89255443484797	37.0	33.0	40.0	22.0	40.0
70-71	33.662526471496506	37.0	33.0	40.0	22.0	40.0
72-73	33.05376186478024	37.0	33.0	40.0	15.0	40.0
74-75	32.86113289213257	37.0	33.0	37.0	15.0	40.0
76-77	32.4814155533161	37.0	33.0	37.0	15.0	40.0
78-79	32.20584274145247	37.0	33.0	37.0	15.0	38.5
80-81	31.90531693813115	37.0	33.0	37.0	15.0	37.0
82-83	31.620048580072776	37.0	33.0	37.0	10.5	37.0
84-85	31.228672243585024	37.0	33.0	37.0	6.0	37.0
86-87	31.041886369555826	37.0	33.0	37.0	4.0	37.0
88-89	30.821439384531523	37.0	33.0	37.0	2.0	37.0
90-91	30.537914546107935	37.0	33.0	37.0	2.0	37.0
92-93	30.25477544697953	33.0	33.0	37.0	2.0	37.0
94-95	29.996012790939194	33.0	33.0	37.0	2.0	37.0
96-97	29.695111688560026	33.0	33.0	37.0	2.0	37.0
98-99	29.36661923685162	33.0	33.0	37.0	2.0	37.0
100-101	28.654035398998396	33.0	27.0	37.0	2.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09531894791138384
1101	2	0.10758231332889423
1101	3	0.18067003127493209
1101	4	0.1476945866622259
1101	5	0.11230440992726187
1101	6	0.24532719406597892
1101	7	0.28419093375001836
1101	8	0.23172179958774564
1101	9	0.19884464771634214
1101	10-11	0.22363649929325646
1101	12-13	0.26499982920944376
1101	14-15	0.27055239781542184
1101	16-17	0.29048143697004036
1101	18-19	0.2886517790984371
1101	20-21	0.35612192598849646
1101	22-23	0.36729294558677594
1101	24-25	0.3063416672660537
1101	26-27	0.33381518149740685
1101	28-29	0.3338829914823336
1101	30-31	0.3217604233411038
1101	32-33	0.2913646181287106
1101	34-35	0.32495635611819296
1101	36-37	0.3430584794343332
1101	38-39	0.3623966072620277
1101	40-41	0.36681169991774
1101	42-43	0.36533146166842556
1101	44-45	0.22356795428418508
1101	46-47	0.05123624166005669
1101	48-49	0.19613428969200442
1101	50-51	0.13467436786874032
1101	52-53	0.2522101111529409
1101	54-55	0.3422769047526657
1101	56-57	0.29956785719726753
1101	58-59	0.3805438332376241
1101	60-61	0.3910780368367952
1101	62-63	0.395903001595606
1101	64-65	0.4529327562081207
1101	66-67	0.45947762402470005
1101	68-69	0.5421224952663479
1101	70-71	0.39319215881116776
1101	72-73	0.23761748818732542
1101	74-75	0.3624612316234561
1101	76-77	0.34154533703392786
1101	78-79	0.3876572544404411
1101	80-81	0.5144503870975008
1101	82-83	0.465494834124609
1101	84-85	0.4932051111233555
1101	86-87	0.48329586409799674
1101	88-89	0.4603115945603662
1101	90-91	0.3613535254189202
1101	92-93	0.39790714996811616
1101	94-95	0.4012581862759106
1101	96-97	0.346684095837702
1101	98-99	0.342041700467167
1101	100-101	0.34945048984034344
1102	1	0.23141664092782221
1102	2	0.2226534553509616
1102	3	0.2973858814984247
1102	4	0.3257723415702287
1102	5	0.1827616931761824
1102	6	0.2504256830743614
1102	7	0.30930647254214705
1102	8	0.26314258523218115
1102	9	0.31514799068903443
1102	10-11	0.34790227701841303
1102	12-13	0.37208449612918315
1102	14-15	0.4224713981857491
1102	16-17	0.4135302505860281
1102	18-19	0.41043607998572185
1102	20-21	0.43836986412942736
1102	22-23	0.3568633034194022
1102	24-25	0.4376237444451476
1102	26-27	0.38615861738870905
1102	28-29	0.4433327064227939
1102	30-31	0.42438306588731933
1102	32-33	0.4070253049881174
1102	34-35	0.41766426499665243
1102	36-37	0.44725124221518797
1102	38-39	0.42993335078428885
1102	40-41	0.503472962584631
1102	42-43	0.5312081343833981
1102	44-45	0.589240250165652
1102	46-47	0.47618584883009163
1102	48-49	0.5509498765067846
1102	50-51	0.35305651907469837
1102	52-53	0.5314364530301248
1102	54-55	0.6493260577390672
1102	56-57	0.6275427077988311
1102	58-59	0.6401711468673454
1102	60-61	0.6298657530983647
1102	62-63	0.6530171554313924
1102	64-65	0.6688884283182546
1102	66-67	0.7677452406189502
1102	68-69	0.7092324330922892
1102	70-71	0.7067081659112446
1102	72-73	0.5651485355494259
1102	74-75	0.5782587308079172
1102	76-77	0.4977928096530846
1102	78-79	0.5494518080290405
1102	80-81	0.5654598979823184
1102	82-83	0.5077892586595745
1102	84-85	0.6021183925024474
1102	86-87	0.5707569787111666
1102	88-89	0.5890669197155312
1102	90-91	0.6298293324104165
1102	92-93	0.6536321392007451
1102	94-95	0.5742570424002729
1102	96-97	0.6542313242417315
1102	98-99	0.6890098785898076
1102	100-101	0.7250921301642066
1103	1	-0.0500221263840821
1103	2	-0.0618077039598397
1103	3	-0.10305396824778157
1103	4	-0.05187302768188573
1103	5	-0.1147744480422972
1103	6	-0.08843628220763833
1103	7	-0.1004377041422373
1103	8	-0.11412892253570561
1103	9	-0.1319169041586008
1103	10-11	-0.11475945474866478
1103	12-13	-0.11683843989487741
1103	14-15	-0.08436408395110817
1103	16-17	-0.08692294621316421
1103	18-19	-0.020006648871920163
1103	20-21	0.003967794553211945
1103	22-23	0.009836820724274276
1103	24-25	0.03133247646881898
1103	26-27	-0.04180842664045059
1103	28-29	-0.07133159179446835
1103	30-31	-4.747357981571554E-4
1103	32-33	-0.0676284793874089
1103	34-35	-0.048433860412906427
1103	36-37	-0.12397643555868854
1103	38-39	-0.10292546466520491
1103	40-41	-0.11504130791701783
1103	42-43	-0.05535056425463836
1103	44-45	-0.007061302151512905
1103	46-47	-0.23370263442718908
1103	48-49	-0.16027214204463291
1103	50-51	-0.13788620496791282
1103	52-53	-0.004277578754582123
1103	54-55	0.05296083016911268
1103	56-57	0.16686570891113917
1103	58-59	-0.026934901543661738
1103	60-61	0.08096882897758206
1103	62-63	0.09345937398700244
1103	64-65	0.11842360797291462
1103	66-67	0.1711790422511399
1103	68-69	0.12103622295334304
1103	70-71	0.11087331044686977
1103	72-73	-0.06719887986981377
1103	74-75	0.053096115912339314
1103	76-77	-0.034071951850016546
1103	78-79	-0.005894541695965927
1103	80-81	0.06546363299714031
1103	82-83	0.1982481497813744
1103	84-85	0.27563993638297646
1103	86-87	0.18432127456141956
1103	88-89	0.11795667440785707
1103	90-91	0.1544474451443243
1103	92-93	-0.0037023454171567494
1103	94-95	0.04646214205451571
1103	96-97	0.16757018719701477
1103	98-99	0.19959266060345016
1103	100-101	0.2842510300250396
1104	1	0.08941102738342721
1104	2	0.07320292769463776
1104	3	0.10869416168701918
1104	4	0.18118452217527903
1104	5	0.085300863725152
1104	6	0.13034709977122105
1104	7	0.08343327012599389
1104	8	0.06296331827851986
1104	9	0.10126464794831236
1104	10-11	0.10362814846571666
1104	12-13	0.024320208128237653
1104	14-15	0.047969498454492054
1104	16-17	0.10914307187649541
1104	18-19	0.15794610517505703
1104	20-21	0.13444678042892377
1104	22-23	0.03585461702810733
1104	24-25	0.10701785232966898
1104	26-27	0.05860376493029662
1104	28-29	-0.005399823210481713
1104	30-31	0.046966366926632475
1104	32-33	0.0020488568116050487
1104	34-35	0.06238082172322379
1104	36-37	0.04492360632052339
1104	38-39	0.017125952012030154
1104	40-41	0.008338654533311285
1104	42-43	0.05633942540031711
1104	44-45	0.024946746951691523
1104	46-47	-0.04873882184103451
1104	48-49	-0.08372916122949192
1104	50-51	-0.014755427188035242
1104	52-53	0.06608741777908733
1104	54-55	0.12627553759461563
1104	56-57	0.08658242910136948
1104	58-59	0.09355735113970809
1104	60-61	0.05777218922779781
1104	62-63	0.07355199717662231
1104	64-65	0.032595084117282624
1104	66-67	0.1136504281630053
1104	68-69	0.07482464763415919
1104	70-71	0.1451174884948827
1104	72-73	0.04507310266905051
1104	74-75	-0.011013763737196314
1104	76-77	-0.12452362688353702
1104	78-79	0.052431151435300194
1104	80-81	0.15042135927300748
1104	82-83	0.13434555873287124
1104	84-85	0.16588057998752603
1104	86-87	0.12939257711948926
1104	88-89	0.028548439107389356
1104	90-91	0.09532294486243131
1104	92-93	-0.04337573141534179
1104	94-95	-0.017820337599335545
1104	96-97	0.00686630625536111
1104	98-99	-0.05826812103389756
1104	100-101	-0.06215161497414101
1105	1	0.012376695726569409
1105	2	-0.050266878520645264
1105	3	0.01603192243095819
1105	4	-0.02687841770863031
1105	5	-0.06342936778666086
1105	6	-0.07048412309649876
1105	7	-0.16221186320615288
1105	8	-0.10808269730684827
1105	9	-0.06989528179772009
1105	10-11	-0.09048476771130964
1105	12-13	-0.018493592723679342
1105	14-15	0.012566570525990528
1105	16-17	-0.007039972088314528
1105	18-19	0.006692794494973953
1105	20-21	0.08295339764675447
1105	22-23	0.05852855359560749
1105	24-25	-0.0014316150300146546
1105	26-27	-0.034539263777979556
1105	28-29	-0.1045494340860813
1105	30-31	0.008534676548819675
1105	32-33	0.023515422766472227
1105	34-35	-0.031619550449477174
1105	36-37	0.028931372557828183
1105	38-39	0.024464294275809095
1105	40-41	0.052596775418066954
1105	42-43	-0.16165471829658173
1105	44-45	-0.04000619136450467
1105	46-47	-0.024806970230876857
1105	48-49	0.03341486418786843
1105	50-51	0.046965814186350485
1105	52-53	0.07760421908220394
1105	54-55	0.047682852253885244
1105	56-57	0.06884659173828567
1105	58-59	0.15315612475419016
1105	60-61	0.031270617371703224
1105	62-63	-0.05331834588503881
1105	64-65	0.039662143392149574
1105	66-67	0.19768020452799817
1105	68-69	0.0714077406059559
1105	70-71	0.10030795934432746
1105	72-73	-0.025531093259480997
1105	74-75	0.12485352769951419
1105	76-77	0.07386800076022837
1105	78-79	0.07465504740793705
1105	80-81	0.1679245488695038
1105	82-83	0.18327380173360197
1105	84-85	0.1942765124713226
1105	86-87	0.09123961796417035
1105	88-89	0.10084951789838925
1105	90-91	0.12083242793749349
1105	92-93	0.11877295094035745
1105	94-95	0.08897068296957755
1105	96-97	0.028515095037157323
1105	98-99	0.0869549085538317
1105	100-101	0.05877977747432439
1106	1	-0.1255127524225621
1106	2	-0.06689435039988112
1106	3	-0.04744952929626578
1106	4	-0.05705217546707786
1106	5	-0.13719535749495293
1106	6	-0.07347096430952149
1106	7	-0.06620082295367524
1106	8	-0.16095306972094647
1106	9	-0.06929678205513312
1106	10-11	-0.04487165289346251
1106	12-13	-0.06213582581698063
1106	14-15	-0.08614791800454213
1106	16-17	-0.06798550924920477
1106	18-19	-0.07052060472777555
1106	20-21	-0.026513169437286876
1106	22-23	-0.08586674054379273
1106	24-25	-0.13401173857548088
1106	26-27	-0.12211839805778624
1106	28-29	-0.060126027750015965
1106	30-31	-0.14686473378032616
1106	32-33	-0.1578520316127694
1106	34-35	-0.11645380124070925
1106	36-37	-0.0747472533995932
1106	38-39	-0.17027386427809432
1106	40-41	-0.03888639236433988
1106	42-43	-0.18147939476527597
1106	44-45	-0.17537319139072594
1106	46-47	-0.25742547712422237
1106	48-49	-0.2207234575410837
1106	50-51	-0.17432271249150944
1106	52-53	-0.1760098675506896
1106	54-55	-0.10561316336018223
1106	56-57	-0.11621501086569452
1106	58-59	-0.07557922560800279
1106	60-61	-0.17947224981578103
1106	62-63	-0.10795071078452878
1106	64-65	-0.15643246052429305
1106	66-67	-0.08746057916960837
1106	68-69	-0.08964777516560218
1106	70-71	-0.08378350155202696
1106	72-73	-0.41402567476903585
1106	74-75	-0.17766876902551587
1106	76-77	-0.34681520530192955
1106	78-79	-0.3347882601800549
1106	80-81	-0.3071120117275363
1106	82-83	-0.0835282757322311
1106	84-85	-0.11662095586121879
1106	86-87	-0.12119890553035617
1106	88-89	-0.16521039120072345
1106	90-91	-0.2552218292395416
1106	92-93	-0.24620651113977132
1106	94-95	-0.1438595020713045
1106	96-97	-0.10491158173226367
1106	98-99	-0.26608934034652165
1106	100-101	-0.31993466005902604
1107	1	-0.05608552167315395
1107	2	-0.036310359344078336
1107	3	-0.04118028078315561
1107	4	0.027437211614547152
1107	5	-0.005553607392087656
1107	6	-0.03432432025271481
1107	7	-0.0347107750324156
1107	8	-0.09790398055746863
1107	9	-0.05037016850501175
1107	10-11	-0.047788491004375544
1107	12-13	0.029975660739708587
1107	14-15	0.02501860942861356
1107	16-17	0.07376500923975726
1107	18-19	0.06932137562990448
1107	20-21	0.09546785324322826
1107	22-23	0.05306377985704103
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2316	84-85	-0.472669580844876
2316	86-87	-0.5381982833829149
2316	88-89	-0.5196053593323384
2316	90-91	-0.41274618058684354
2316	92-93	-0.47862000176088415
2316	94-95	-0.4175702321756525
2316	96-97	-0.38959231617585743
2316	98-99	-0.3749869070434393
2316	100-101	-0.3914067016667282
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84353.0
3	12647.0
4	9588.0
5	8317.0
6	8234.0
7	8948.0
8	9065.0
9	9223.0
10	9530.0
11	9473.0
12	10041.0
13	10423.0
14	10710.0
15	11894.0
16	13477.0
17	16847.0
18	15506.0
19	12788.0
20	13210.0
21	14057.0
22	15507.0
23	18085.0
24	21278.0
25	24656.0
26	27855.0
27	33515.0
28	39928.0
29	49279.0
30	61546.0
31	77595.0
32	99894.0
33	133479.0
34	186021.0
35	271042.0
36	433197.0
37	847766.0
38	1000275.0
39	515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.18541702841292	24.38471082645191	13.75871213203537	22.671160013099794
2	13.52227143343847	22.069353012935345	44.40178149487312	20.006594058753063
3	16.880645976657803	31.34272589401499	30.615205398158118	21.16142273116909
4	10.755345527478037	18.803563456030332	43.53441537478793	26.906675641703696
5	11.599736900380966	38.94783780798674	37.0289909624072	12.423434329225104
6	25.031595455089946	42.32562880450491	20.95336400931489	11.689411731090257
7	23.36505883348481	32.800670592928554	28.08250754719262	15.75176302639402
8	22.294714712272555	38.87507541697758	22.277763063759078	16.552446806990783
9	25.350599654263302	16.131403025031293	24.03801455259187	34.47998276811354
10-11	22.287108961275205	28.216468059303896	31.425112802736223	18.071310176684676
12-13	22.72044473243645	25.702714312462504	30.83961682654207	20.737224128558974
14-15	20.458983657260625	27.449161363584224	27.920431989143946	24.17142299001121
16-17	19.42042269397034	29.619735335100845	29.613058746969262	21.346783223959555
18-19	19.16802429708539	30.473592869132943	30.62653493289878	19.731847900882883
20-21	20.024828532838942	29.110582444356282	29.95060943511722	20.913979587687553
22-23	20.06676806518225	29.903820687275328	29.329882376989275	20.699528870553145
24-25	20.053132580335586	29.43969154035579	29.886039283326966	20.621136595981664
26-27	20.30942116027303	29.145364919263994	29.33789113799686	21.207322782466115
28-29	19.999414797223118	30.200488273415527	29.00376233184349	20.79633459751786
30-31	21.198008991791774	29.49600578499045	28.736066404305333	20.569918818912434
32-33	20.899418204037403	29.56974682973951	29.019381476381433	20.51145348984165
34-35	21.251982271378033	28.718166892139163	29.576175817992596	20.453675018490213
36-37	21.407802813589015	29.4232126038941	29.1837327914667	19.98525179105019
38-39	22.030164593089	28.68001881440665	29.8497100361452	19.440106556359147
40-41	20.86087065702463	28.697094338990215	30.226743158952647	20.215291845032514
42-43	21.46356192324557	28.75641112995238	29.297819858650175	20.482207088151867
44-45	20.922495944083995	29.505913188973405	29.238037280084185	20.333553586858418
46-47	21.758413988114263	28.766267606432898	28.576617821295518	20.898700584157318
48-49	21.65770283381957	27.78463656221889	29.60161141846751	20.95604918549403
50-51	21.52280219924211	28.62556747227017	28.427929745850218	21.423700582637505
52-53	20.457043171513728	28.6865108885736	29.273335596660253	21.58311034325241
54-55	21.056176616116577	29.286831663108455	28.466558518973855	21.190433201801113
56-57	21.468303902159366	28.14989018335199	29.138260682305763	21.243545232182885
58-59	20.9020412206679	27.96706953922734	30.178076184206553	20.952813055898208
60-61	21.0456449482281	29.733199444056456	28.38002239752718	20.84113321018826
62-63	21.458062189684142	28.820093488516683	27.772937805770493	21.94890651602868
64-65	20.29365291867895	28.825597679909997	28.516248673628258	22.36450072778279
66-67	21.05649200957906	27.657139803755744	29.06825911822154	22.21810906844366
68-69	20.12137804854709	28.488303969164352	28.590537788442223	22.799780193846335
70-71	20.26452246802258	28.25917666021966	29.205255196222957	22.271045675534804
72-73	20.925220251627515	28.333937141869526	29.256268529720675	21.484574076782287
74-75	21.22193986769235	29.131258027538777	28.039968511331775	21.6068335934371
76-77	20.501891982343803	29.138795183321083	29.109931272651018	21.2493815616841
78-79	20.613208385999364	28.673044811010907	28.554062886034725	22.159683916955
80-81	20.663251070307613	28.938601800924918	29.299043071760455	21.09910405700701
82-83	21.45551875918962	28.09258577334844	29.329266033041783	21.122629434420155
84-85	22.101139882726343	28.08948800532714	28.42042303315508	21.388949078791438
86-87	21.314305805031275	28.92338451627172	28.524546230385333	21.237763448311668
88-89	21.945754015385056	27.744911495162146	28.746567999876603	21.562766489576195
90-91	21.658567706421564	27.558740933863536	29.249846805897807	21.5328445538171
92-93	21.27371508769843	28.498306719126848	28.746165370719613	21.481812822455115
94-95	20.736858099515427	27.924329540937727	29.109861476446813	22.228950883100037
96-97	21.33616310466655	27.930005394211477	28.441499356918094	22.29233214420388
98-99	20.654841417439425	28.701625278110924	28.772605391159033	21.870927913290615
100-101	21.407761607342103	27.37649318034171	28.333562173232448	22.882183039083746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	56.5
2	52.0
3	68.5
4	51.5
5	52.0
6	72.5
7	130.0
8	209.5
9	235.0
10	218.0
11	207.5
12	266.5
13	446.5
14	599.5
15	744.0
16	972.5
17	1146.5
18	1220.5
19	1298.5
20	1427.5
21	1546.5
22	1743.0
23	2041.0
24	2321.0
25	2714.5
26	3308.0
27	4363.5
28	7139.5
29	14131.0
30	26215.5
31	45709.0
32	74542.5
33	115326.5
34	168767.5
35	212294.0
36	250163.0
37	291108.0
38	287663.5
39	237147.5
40	188486.0
41	149316.5
42	120691.5
43	104694.0
44	91748.5
45	85521.0
46	84740.0
47	85898.5
48	88304.5
49	86927.0
50	83118.0
51	81478.0
52	78176.0
53	74239.0
54	70449.0
55	64312.5
56	58028.5
57	49173.5
58	40673.0
59	37142.0
60	32106.5
61	25306.0
62	20078.5
63	16561.0
64	13380.5
65	9704.5
66	7408.0
67	6071.5
68	5049.5
69	4283.0
70	3517.5
71	2788.5
72	2284.0
73	1875.5
74	1514.0
75	1260.0
76	1069.0
77	891.0
78	692.0
79	540.0
80	450.5
81	375.5
82	311.5
83	256.0
84	203.0
85	181.5
86	163.0
87	129.0
88	97.0
89	73.0
90	63.5
91	55.0
92	42.5
93	31.0
94	26.0
95	19.5
96	11.5
97	8.0
98	5.5
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	3.296917052863867E-4
2	0.0034068142879593293
3	1.0989723509546223E-4
4	0.0010714980421807569
5	0.0021429960843615137
6	0.0
7	0.0
8	0.0
9	0.0
10-11	8.242292632159668E-5
12-13	6.86857719346639E-5
14-15	0.003722768838858783
16-17	0.004959112733682733
18-19	0.004134883470466767
20-21	0.0
22-23	0.0
24-25	2.7474308773865558E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	1.9232016141705892E-4
42-43	0.0
44-45	5.220118667034456E-4
46-47	0.7287560402267839
48-49	0.5248417205071538
50-51	0.4037899160495021
52-53	0.3395961935993652
54-55	0.3299389740653515
56-57	0.29563729956118034
58-59	0.29626920866297923
60-61	0.27196818255249516
62-63	0.26629473779069196
64-65	0.2626543918781547
66-67	0.2509915478036488
68-69	0.23030339329692803
70-71	0.24401307337508696
72-73	0.8063160138954063
74-75	0.5333862305358259
76-77	0.6261532341107829
78-79	0.35009137955098185
80-81	0.30269819691606376
82-83	0.2837958724796443
84-85	0.2760755917141881
86-87	0.2498651011439203
88-89	0.25353292136523137
90-91	0.262805500576411
92-93	0.2213605057910348
94-95	0.18902324436419504
96-97	0.1459572653611608
98-99	0.15516115880040574
100-101	0.1508614294772958
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3639764.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.715360961091456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.58453660401538	32.40313134753682
2	11.203947400411513	9.123455251936742
3	3.7631983809280616	4.596599413430429
4	1.6974604269122446	2.7645085599560177
5	0.9101643174880402	1.85288343602155
6	0.5597409856135281	1.367403376438314
7	0.392685008723021	1.1191818311917003
8	0.2591667637129283	0.8441654666951818
9	0.1948170839159576	0.7138843103722913
>10	1.116440809604557	8.785580179410827
>50	0.14384562642651932	4.168110494099128
>100	0.14476206686474363	12.192130450172376
>500	0.01633892642692082	4.68731279562926
>1k	0.011072660884282793	8.452326226320551
>5k	0.0011477758233707773	3.230920427215654
>10k+	6.751622490416338E-4	3.6984064335732683
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	17497	0.4807179806163257	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	17151	0.47121186978056817	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	16610	0.45634826873390694	No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC	13885	0.3814807773251233	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	13111	0.36021566233415137	No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC	12970	0.35634178479703627	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	11521	0.3165315113837051	No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG	11156	0.30650338868124416	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	10439	0.28680430929038253	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	10199	0.2802104751846548	No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA	9784	0.2688086370435006	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	9638	0.2647973879625163	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	8970	0.24644454970157406	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	8736	0.2400155614484895	No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA	8184	0.22484974300531574	No Hit
TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA	7144	0.19627646188049552	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	7028	0.19308944206272713	No Hit
ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA	6469	0.17773130345813629	No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA	6402	0.1758905247702873	No Hit
TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG	6260	0.1719891729243984	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6234	0.1712748408962779	No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT	6022	0.1654502874362184	No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG	5664	0.15561448489517452	No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT	5395	0.1482238958350047	No Hit
TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG	5275	0.1449269787821408	No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG	5234	0.14380053212241234	No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC	5094	0.13995412889407113	No Hit
ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT	4827	0.13261848845144905	No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA	4725	0.12981610895651474	No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC	4621	0.12695878084403275	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4532	0.1245135673631587	No Hit
GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG	4442	0.12204087957351081	No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG	4155	0.1141557529554114	No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA	4094	0.11247982012020559	No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA	3649	0.10025375271583542	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.7474308773865558E-5	0.0
5	0.0	0.0	0.0	2.7474308773865558E-5	0.0
6	0.0	0.0	0.0	2.7474308773865558E-5	0.0
7	0.0	0.0	0.0	2.7474308773865558E-5	0.0
8	0.0	0.0	0.0	2.7474308773865558E-5	2.7474308773865558E-5
9	0.0	0.0	0.0	2.7474308773865558E-5	2.7474308773865558E-5
10-11	0.0	0.0	0.0	2.7474308773865558E-5	2.7474308773865558E-5
12-13	0.0	0.0	0.0	5.4948617547731115E-5	2.7474308773865558E-5
14-15	0.0	0.0	0.0	5.4948617547731115E-5	4.121146316079834E-5
16-17	0.0	0.0	0.0	6.868577193466389E-5	5.4948617547731115E-5
18-19	0.0	0.0	0.0	8.242292632159668E-5	5.4948617547731115E-5
20-21	0.0	0.0	0.0	1.0989723509546223E-4	5.4948617547731115E-5
22-23	0.0	0.0	0.0	1.9232016141705892E-4	5.4948617547731115E-5
24-25	0.0	0.0	0.0	4.808004035426473E-4	5.4948617547731115E-5
26-27	0.0	0.0	0.0	0.0010714980421807569	5.4948617547731115E-5
28-29	0.0	0.0	0.0	0.00306338542828601	5.4948617547731115E-5
30-31	0.0	0.0	0.0	0.012432124720174164	5.4948617547731115E-5
32-33	0.0	0.0	0.0	0.045593615410229896	5.4948617547731115E-5
34-35	0.0	0.0	0.0	0.10106424482466446	5.4948617547731115E-5
36-37	0.0	0.0	0.0	0.1851905782902408	5.4948617547731115E-5
38-39	1.3737154386932779E-5	0.0	0.0	0.30470382145655595	5.4948617547731115E-5
40-41	2.7474308773865558E-5	0.0	0.0	0.4321159283953575	5.4948617547731115E-5
42-43	2.7474308773865558E-5	0.0	0.0	0.5755318201949358	5.4948617547731115E-5
44-45	2.7474308773865558E-5	0.0	0.0	0.7414629080346967	5.4948617547731115E-5
46-47	2.7474308773865558E-5	0.0	0.0	0.9409950755048954	5.4948617547731115E-5
48-49	2.7474308773865558E-5	0.0	0.0	1.14650290513341	5.4948617547731115E-5
50-51	2.7474308773865558E-5	0.0	0.0	1.353439398818165	5.4948617547731115E-5
52-53	2.7474308773865558E-5	0.0	0.0	1.5633705921592718	5.4948617547731115E-5
54-55	2.7474308773865558E-5	0.0	0.0	1.7898825308454063	5.4948617547731115E-5
56-57	2.7474308773865558E-5	0.0	0.0	2.0596390315416055	6.868577193466389E-5
58-59	2.7474308773865558E-5	2.7474308773865558E-5	0.0	2.37200818514607	8.242292632159668E-5
60-61	2.7474308773865558E-5	5.4948617547731115E-5	0.0	2.6931279060950106	8.242292632159668E-5
62-63	2.7474308773865558E-5	5.4948617547731115E-5	0.0	3.016390623128313	8.242292632159668E-5
64-65	2.7474308773865558E-5	5.4948617547731115E-5	0.0	3.3554098562434267	8.242292632159668E-5
66-67	2.7474308773865558E-5	8.242292632159668E-5	0.0	3.7294038844276716	8.242292632159668E-5
68-69	2.7474308773865558E-5	8.242292632159668E-5	0.0	4.133825709578973	8.242292632159668E-5
70-71	2.7474308773865558E-5	9.616008070852946E-5	0.0	4.556627847299989	8.242292632159668E-5
72-73	2.7474308773865558E-5	1.0989723509546223E-4	0.0	5.029680495768407	8.242292632159668E-5
74-75	2.7474308773865558E-5	1.0989723509546223E-4	0.0	5.464681226585021	8.242292632159668E-5
76-77	2.7474308773865558E-5	1.0989723509546223E-4	0.0	5.9542596717809175	8.242292632159668E-5
78-79	2.7474308773865558E-5	1.0989723509546223E-4	0.0	6.5055591516373035	8.242292632159668E-5
80-81	2.7474308773865558E-5	1.0989723509546223E-4	0.0	7.1171510020979385	8.242292632159668E-5
82-83	2.7474308773865558E-5	1.0989723509546223E-4	0.0	7.773223758463461	8.242292632159668E-5
84-85	2.7474308773865558E-5	1.0989723509546223E-4	0.0	8.398621449082963	8.242292632159668E-5
86-87	5.4948617547731115E-5	1.0989723509546223E-4	0.0	9.076289012144745	8.242292632159668E-5
88-89	5.4948617547731115E-5	1.23634389482395E-4	0.0	9.779645053910087	8.242292632159668E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTGGG	4065	0.0	56.85763	3
TACCTGG	4090	0.0	55.1258	2
CCTGGGG	3405	0.0	48.823112	4
GTACCTG	4830	0.0	47.758766	1
GTACCCG	385	0.0	45.52105	1
GTACACG	470	0.0	42.327507	1
CTGGGGG	3160	0.0	41.36676	5
TGGGGGG	6110	0.0	32.556046	6
TATTCGC	830	0.0	31.955923	9
GCATCGT	1445	0.0	27.859968	8
GGGGGGA	6360	0.0	26.659346	7
TATCACG	180	6.9139787E-9	26.31472	2
CATGGGG	5405	0.0	25.32431	4
GTTATAA	6575	0.0	25.214106	1
CTGTGCG	1840	0.0	24.711258	9
GGGGAAT	6880	0.0	24.507727	9
ATGGGGG	4620	0.0	24.398577	5
GTACTAG	1970	0.0	24.284344	1
TATTCTC	3005	0.0	24.27201	5
CGTTAAC	430	0.0	24.234022	1
>>END_MODULE