##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931065_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3639764 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.61968825451321 33.0 33.0 33.0 33.0 33.0 2 32.59449980822932 33.0 33.0 33.0 33.0 33.0 3 32.643695305519806 33.0 33.0 33.0 33.0 33.0 4 36.58477472715264 37.0 37.0 37.0 37.0 37.0 5 36.52913430650998 37.0 37.0 37.0 37.0 37.0 6 36.535183050329636 37.0 37.0 37.0 37.0 37.0 7 36.47499508209873 37.0 37.0 37.0 37.0 37.0 8 36.48931826349181 37.0 37.0 37.0 37.0 37.0 9 36.484122047473406 37.0 37.0 37.0 37.0 37.0 10-11 36.4611054452981 37.0 37.0 37.0 37.0 37.0 12-13 36.39003710130657 37.0 37.0 37.0 37.0 37.0 14-15 38.37737872565364 40.0 37.0 40.0 37.0 40.0 16-17 38.37577669870904 40.0 37.0 40.0 37.0 40.0 18-19 38.32990037266153 40.0 37.0 40.0 37.0 40.0 20-21 38.259757638132584 40.0 37.0 40.0 37.0 40.0 22-23 38.14362991666493 40.0 37.0 40.0 35.0 40.0 24-25 38.289861925113826 40.0 37.0 40.0 37.0 40.0 26-27 38.2283321116424 40.0 37.0 40.0 37.0 40.0 28-29 38.16537253514239 40.0 37.0 40.0 37.0 40.0 30-31 38.0689191661877 40.0 37.0 40.0 33.0 40.0 32-33 37.959960590851495 40.0 37.0 40.0 33.0 40.0 34-35 37.90222855108188 40.0 37.0 40.0 33.0 40.0 36-37 37.86943288081315 40.0 37.0 40.0 33.0 40.0 38-39 37.76999470295327 40.0 37.0 40.0 33.0 40.0 40-41 37.627260998240544 40.0 37.0 40.0 33.0 40.0 42-43 37.51363632367374 40.0 37.0 40.0 33.0 40.0 44-45 37.36444753011459 40.0 37.0 40.0 33.0 40.0 46-47 37.207917463879525 40.0 37.0 40.0 33.0 40.0 48-49 37.049821774158985 40.0 37.0 40.0 33.0 40.0 50-51 36.89037558479066 40.0 37.0 40.0 33.0 40.0 52-53 36.71355285672368 37.0 37.0 40.0 33.0 40.0 54-55 36.54262886275044 37.0 37.0 40.0 33.0 40.0 56-57 36.29975885799189 37.0 37.0 40.0 33.0 40.0 58-59 36.085999943952416 37.0 37.0 40.0 33.0 40.0 60-61 35.88082839986329 37.0 37.0 40.0 33.0 40.0 62-63 35.61296062052375 37.0 37.0 40.0 27.0 40.0 64-65 35.29987672277653 37.0 33.0 40.0 27.0 40.0 66-67 35.01526857235799 37.0 33.0 40.0 27.0 40.0 68-69 34.76815076471991 37.0 33.0 40.0 27.0 40.0 70-71 34.50761422993359 37.0 33.0 38.5 27.0 40.0 72-73 34.16296166454748 37.0 33.0 37.0 27.0 40.0 74-75 33.77137885862929 37.0 33.0 37.0 27.0 40.0 76-77 32.15133151490042 33.0 33.0 37.0 24.5 37.0 78-79 32.88302579507902 37.0 33.0 37.0 27.0 37.0 80-81 32.86816466672015 37.0 33.0 37.0 27.0 37.0 82-83 32.60357141287183 37.0 33.0 37.0 27.0 37.0 84-85 32.25955844939397 35.0 33.0 37.0 24.5 37.0 86-87 31.88330726937241 33.0 33.0 37.0 22.0 37.0 88-89 31.510965546117824 33.0 33.0 37.0 22.0 37.0 90-91 31.09934215515072 33.0 33.0 37.0 22.0 37.0 92-93 30.70368353552593 33.0 33.0 37.0 15.0 37.0 94-95 30.2346947494398 33.0 33.0 37.0 15.0 37.0 96-97 29.737659089984955 33.0 30.0 37.0 4.0 37.0 98-99 29.09407285747098 33.0 27.0 37.0 2.0 37.0 100-101 27.34039487175542 33.0 24.5 35.0 2.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.006493525775859155 1101 2 0.15218422050080704 1101 3 0.061102617363374634 1101 4 0.08936796252641699 1101 5 0.04859415374180287 1101 6 0.06753449118267696 1101 7 0.06575081285898676 1101 8 0.10057165013292746 1101 9 0.11053516037886624 1101 10-11 0.14369177913783204 1101 12-13 0.13740827588689797 1101 14-15 0.1964754086116116 1101 16-17 0.22086938154232172 1101 18-19 0.1566111996209898 1101 20-21 0.11600779309606679 1101 22-23 0.11793847527477652 1101 24-25 0.2070044273108138 1101 26-27 0.1761180413578174 1101 28-29 0.18633799423596287 1101 30-31 0.2021825211126682 1101 32-33 0.20881869920654594 1101 34-35 0.17788458635097726 1101 36-37 0.0333401669829243 1101 38-39 0.06851546358478089 1101 40-41 0.03394092499908652 1101 42-43 0.052268501677531276 1101 44-45 0.019722617698910483 1101 46-47 -0.07926101864723734 1101 48-49 -0.12624217162804996 1101 50-51 -0.050188546078480556 1101 52-53 -0.10032519285216068 1101 54-55 -0.15125361208628618 1101 56-57 -0.1741606289431772 1101 58-59 -0.26083619954922455 1101 60-61 -0.2040589310869123 1101 62-63 -0.2499720276985613 1101 64-65 -0.23009934934903242 1101 66-67 -0.2510879406806694 1101 68-69 -0.3992828192329867 1101 70-71 -0.35039627578821353 1101 72-73 -0.40517378261701253 1101 74-75 -0.6056959100832131 1101 76-77 -0.5799475895564363 1101 78-79 -0.45683024103870196 1101 80-81 -0.7759320433583525 1101 82-83 -0.8292714671549533 1101 84-85 -0.8625147317084796 1101 86-87 -0.8923762836553841 1101 88-89 -1.0659284575460681 1101 90-91 -1.219134459229597 1101 92-93 -1.251428206427235 1101 94-95 -1.4749304756917638 1101 96-97 -1.5408522718590483 1101 98-99 -1.6512994476590706 1101 100-101 -3.3884235194887786 1102 1 0.0984258166736538 1102 2 0.20533487039321585 1102 3 0.15121465216567742 1102 4 0.16096351038905965 1102 5 0.126565956975071 1102 6 0.161959858134189 1102 7 0.12930769339092762 1102 8 0.10282650918586 1102 9 0.09372268439502562 1102 10-11 0.08676360183556397 1102 12-13 0.12486366338251287 1102 14-15 0.18025441230212635 1102 16-17 0.14337969132133566 1102 18-19 0.19345252789432976 1102 20-21 0.08064245523676306 1102 22-23 0.13881439920782412 1102 24-25 0.21297615663331015 1102 26-27 0.16631286783582766 1102 28-29 0.14255219733270508 1102 30-31 0.20617858836295966 1102 32-33 0.20770263684751455 1102 34-35 0.17822956779009047 1102 36-37 0.17135494488105252 1102 38-39 0.14791986851211902 1102 40-41 0.1706384658599447 1102 42-43 0.08429455271001984 1102 44-45 -0.07035691184159987 1102 46-47 0.05339663090502711 1102 48-49 0.078212926028975 1102 50-51 0.0747899772306937 1102 52-53 -0.06614997323848826 1102 54-55 -0.07434403437219572 1102 56-57 -0.029564793176795945 1102 58-59 -0.1531804654856046 1102 60-61 -0.2617514173795641 1102 62-63 -0.3116272536739757 1102 64-65 -0.3084406580805208 1102 66-67 -0.27364939787607767 1102 68-69 -0.29879797810052366 1102 70-71 -0.32697473517038134 1102 72-73 -0.3813624207123283 1102 74-75 -0.3988402601463079 1102 76-77 -0.43168533439554935 1102 78-79 -0.29582248892073437 1102 80-81 -0.4603809746898264 1102 82-83 -0.4858944916379997 1102 84-85 -0.6202968880224162 1102 86-87 -0.532876021098005 1102 88-89 -0.8479564434155655 1102 90-91 -0.9464449641949635 1102 92-93 -0.891382851912855 1102 94-95 -1.0359750187355026 1102 96-97 -1.0974908524316582 1102 98-99 -1.06194083838896 1102 100-101 -1.8861915882544302 1103 1 0.0750154637345517 1103 2 0.17003286024423403 1103 3 0.1403849991719568 1103 4 0.1350624875620028 1103 5 0.12505062095918618 1103 6 0.09836659566893502 1103 7 0.14279199692361288 1103 8 0.08995195807388967 1103 9 0.10118740402996451 1103 10-11 0.13009985388048761 1103 12-13 0.11740978879063135 1103 14-15 0.1975057257898385 1103 16-17 0.1908849399171757 1103 18-19 0.1745113226977466 1103 20-21 0.13318538383219192 1103 22-23 0.15339432209011505 1103 24-25 0.19311726279349983 1103 26-27 0.13996094321868924 1103 28-29 0.1596256251142094 1103 30-31 0.18725992825587667 1103 32-33 0.19758515668112153 1103 34-35 0.17141051071842384 1103 36-37 0.14570844306443576 1103 38-39 0.06659473565134277 1103 40-41 0.05500564679764608 1103 42-43 0.03332946537862114 1103 44-45 0.03490750605813986 1103 46-47 -0.068914169668858 1103 48-49 0.06874556344354943 1103 50-51 -0.014650037846166697 1103 52-53 -0.032629070160503204 1103 54-55 -0.043368521399408166 1103 56-57 0.011166128524969565 1103 58-59 -0.15002905797451405 1103 60-61 -0.029764336390847745 1103 62-63 -0.1458317454658129 1103 64-65 -0.19560073010601542 1103 66-67 -0.12505237294588767 1103 68-69 -0.27941572615298327 1103 70-71 -0.26164937082867823 1103 72-73 -0.38293121694044885 1103 74-75 -0.3722294945015747 1103 76-77 -0.43404860877775064 1103 78-79 -0.3428519956278677 1103 80-81 -0.4474176832255523 1103 82-83 -0.45281000029256546 1103 84-85 -0.5405315063538509 1103 86-87 -0.5246232133343582 1103 88-89 -0.706173750538273 1103 90-91 -0.7977042986945229 1103 92-93 -0.6002731862074846 1103 94-95 -0.6647604450136448 1103 96-97 -0.769004938342583 1103 98-99 -0.7438550505883796 1103 100-101 -1.3735317329948096 1104 1 0.05427505481542738 1104 2 0.1288236503248612 1104 3 0.09248708598398281 1104 4 0.14852908130534104 1104 5 0.09311232839007744 1104 6 0.04752693774317862 1104 7 0.12583327728098226 1104 8 0.1556113600576552 1104 9 0.09420972746964651 1104 10-11 0.10749018059227211 1104 12-13 0.08896876429432865 1104 14-15 0.1583626158977154 1104 16-17 0.05852788053906721 1104 18-19 0.10197127106695802 1104 20-21 0.12284122743188419 1104 22-23 0.1408278616049401 1104 24-25 0.09366332790117582 1104 26-27 0.11502688105672121 1104 28-29 0.12646665642179045 1104 30-31 0.1516886479524615 1104 32-33 0.16314793638164105 1104 34-35 0.14184608990587577 1104 36-37 0.15470842687923891 1104 38-39 0.14032949964411756 1104 40-41 0.14233876219274322 1104 42-43 0.1481960959432982 1104 44-45 0.1685730378499244 1104 46-47 0.19201075729542083 1104 48-49 0.16574465842101205 1104 50-51 0.2860259556554752 1104 52-53 0.09756060523178434 1104 54-55 0.14173122159306928 1104 56-57 0.22850384321493067 1104 58-59 0.1388210323254171 1104 60-61 0.2236962620839691 1104 62-63 0.10536402062025729 1104 64-65 0.2618191227974833 1104 66-67 0.32338937145470936 1104 68-69 0.12969589340575993 1104 70-71 0.03320989715510336 1104 72-73 0.0053127038302918095 1104 74-75 0.011471020929668896 1104 76-77 -0.09818965337068164 1104 78-79 0.08869336744666612 1104 80-81 0.054192407168798695 1104 82-83 0.13845080838755308 1104 84-85 0.09028188939591075 1104 86-87 0.163688069909373 1104 88-89 0.10327474882376109 1104 90-91 -0.005600645627023226 1104 92-93 0.09533737018580624 1104 94-95 0.07170476422055216 1104 96-97 0.03354457657342991 1104 98-99 0.08268392607525854 1104 100-101 -0.5788544642341336 1105 1 0.06289899405950905 1105 2 0.1533166287515897 1105 3 0.08503933036379152 1105 4 0.07595715765682343 1105 5 0.03060436236486197 1105 6 0.08219643372957819 1105 7 0.12875521533207035 1105 8 0.08125528574037588 1105 9 0.08837429535419972 1105 10-11 0.08208433550533556 1105 12-13 0.09289290797848082 1105 14-15 0.10403093832927368 1105 16-17 0.15922716213103882 1105 18-19 0.07052841359635664 1105 20-21 0.03975610216772907 1105 22-23 0.05535559739291074 1105 24-25 0.13183978683089492 1105 26-27 0.18679367853614792 1105 28-29 0.12328928777198911 1105 30-31 0.14165057991113628 1105 32-33 0.18131891787242438 1105 34-35 0.1010799956671562 1105 36-37 0.07080610691777878 1105 38-39 0.12564210081264093 1105 40-41 0.1515437831465718 1105 42-43 0.11562931197425996 1105 44-45 0.10050402944799686 1105 46-47 0.01563523185639326 1105 48-49 0.03727099346310325 1105 50-51 0.1579938873057074 1105 52-53 -0.030873747391645168 1105 54-55 0.026870736965584285 1105 56-57 0.05562177830648096 1105 58-59 0.035703584117328546 1105 60-61 -0.05021870155271557 1105 62-63 -0.1667113488121288 1105 64-65 -0.002283529952642027 1105 66-67 0.19421616999201774 1105 68-69 -0.009438742935635958 1105 70-71 -0.0611630061870585 1105 72-73 -0.07573519014382413 1105 74-75 0.0431773546844596 1105 76-77 -0.09013201885956335 1105 78-79 0.058377224583338716 1105 80-81 0.030537454595318536 1105 82-83 0.00466830541144958 1105 84-85 0.014797945397710066 1105 86-87 -0.05433200319962239 1105 88-89 0.05029136085411423 1105 90-91 -0.07864826047161344 1105 92-93 -0.09049446447264486 1105 94-95 -0.052069344158173436 1105 96-97 0.17107246562772715 1105 98-99 0.12685505796459395 1105 100-101 -0.7045809259041711 1106 1 0.1255926190053529 1106 2 0.23657741726523795 1106 3 0.08897288645388102 1106 4 0.09884437428082293 1106 5 0.10878176769675463 1106 6 0.11107629735673896 1106 7 0.13260614005042726 1106 8 0.064318882493545 1106 9 0.10126295663275187 1106 10-11 0.11855556795503475 1106 12-13 0.15721808417972483 1106 14-15 0.2481414849210566 1106 16-17 0.20376897754945134 1106 18-19 0.154582805915922 1106 20-21 0.1441805220727943 1106 22-23 0.17418241837280135 1106 24-25 0.20519497033190248 1106 26-27 0.229558361680688 1106 28-29 0.26550285871181956 1106 30-31 0.22560186230301582 1106 32-33 0.14325836620881205 1106 34-35 0.08178572973454834 1106 36-37 0.07829452517881919 1106 38-39 0.19336137853635904 1106 40-41 0.17181599973033457 1106 42-43 0.23342180651177813 1106 44-45 0.1726008176375018 1106 46-47 0.26582876217857176 1106 48-49 0.1696791608849466 1106 50-51 0.10770154197477666 1106 52-53 0.11012525394009742 1106 54-55 0.08399782027020564 1106 56-57 0.2094374073969547 1106 58-59 0.09873163902185667 1106 60-61 0.018103338959278403 1106 62-63 0.09294905138985143 1106 64-65 0.2979675594547402 1106 66-67 0.2773080303397393 1106 68-69 0.2651649472045321 1106 70-71 0.29002645926127 1106 72-73 0.36067558674007927 1106 74-75 0.3496599411786647 1106 76-77 0.2356306898175191 1106 78-79 0.3846293866845727 1106 80-81 0.2966299142396167 1106 82-83 0.3038372585181932 1106 84-85 0.5460588594919216 1106 86-87 0.5555674423096129 1106 88-89 0.4336580960137155 1106 90-91 0.5279626237772916 1106 92-93 0.5882345630357904 1106 94-95 0.6388730702221359 1106 96-97 0.7848112818062347 1106 98-99 0.8825248489789388 1106 100-101 0.7597028788330356 1107 1 0.0745400552384794 1107 2 0.1163395149946993 1107 3 0.059017316490077576 1107 4 0.06418811479943543 1107 5 0.07380381646589029 1107 6 0.07857794945583407 1107 7 0.05668688717327797 1107 8 0.025286950223375015 1107 9 0.03767605206380864 1107 10-11 0.06042911067893897 1107 12-13 0.06854421062037375 1107 14-15 0.030803380242396372 1107 16-17 0.02666977263513104 1107 18-19 0.05661644639422292 1107 20-21 0.059624635404709636 1107 22-23 0.13111687056167654 1107 24-25 0.06253631686540473 1107 26-27 0.07432048019127535 1107 28-29 0.0998511419834287 1107 30-31 0.04292417332988663 1107 32-33 -0.01920677346110722 1107 34-35 0.04109671089438649 1107 36-37 0.034621337396458784 1107 38-39 0.08285818447810556 1107 40-41 0.13953294424058527 1107 42-43 0.07865981268793121 1107 44-45 0.10960742187767636 1107 46-47 0.10180296033902181 1107 48-49 0.1465298235948893 1107 50-51 0.12157003073011907 1107 52-53 0.13337891245641487 1107 54-55 0.20302917066899084 1107 56-57 0.22159768802521285 1107 58-59 0.2049755345676303 1107 60-61 0.1718054956239925 1107 62-63 -0.0013984837614131607 1107 64-65 0.13488189551870988 1107 66-67 0.2305014651680679 1107 68-69 0.28573243805045223 1107 70-71 0.26879854439737016 1107 72-73 0.27312181559009474 1107 74-75 0.3318181021326083 1107 76-77 0.09925915662799412 1107 78-79 0.28430021373161907 1107 80-81 0.3711796912581917 1107 82-83 0.35170947956281395 1107 84-85 0.49875396304425124 1107 86-87 0.3647697382078299 1107 88-89 0.4469251791340412 1107 90-91 0.5504515370493621 1107 92-93 0.5731025821377962 1107 94-95 0.5512104059993312 1107 96-97 0.617004562854266 1107 98-99 0.8870563801734797 1107 100-101 1.5201751090213556 1108 1 0.10164234563091412 1108 2 0.07404365359716536 1108 3 0.07640521550563051 1108 4 0.03854419200649062 1108 5 0.10976092426270867 1108 6 0.034006372606512514 1108 7 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-0.37732127922361514 2316 60-61 -0.37311271020676884 2316 62-63 -0.4058830618406759 2316 64-65 -0.4947543807991863 2316 66-67 -0.3345070307783118 2316 68-69 -0.36634098833339124 2316 70-71 -0.32900694525856267 2316 72-73 -0.1431893451892705 2316 74-75 -0.21107047738600215 2316 76-77 -0.21163442116889186 2316 78-79 -0.14913142329958617 2316 80-81 -0.08434718064913227 2316 82-83 -0.011856008234083504 2316 84-85 -0.030843407261414768 2316 86-87 0.03039668130788087 2316 88-89 -0.09391120506441197 2316 90-91 -0.02174724767469627 2316 92-93 0.048288221911075624 2316 94-95 0.07615845503006113 2316 96-97 0.3034755516444072 2316 98-99 0.2748256326639904 2316 100-101 0.707740501049603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 28.0 5 44.0 6 81.0 7 182.0 8 545.0 9 1099.0 10 1902.0 11 2802.0 12 3930.0 13 5082.0 14 6160.0 15 7662.0 16 9001.0 17 11052.0 18 11818.0 19 11311.0 20 11752.0 21 12970.0 22 14153.0 23 17000.0 24 19780.0 25 23922.0 26 29191.0 27 35894.0 28 45122.0 29 56634.0 30 71801.0 31 92689.0 32 120823.0 33 163219.0 34 231340.0 35 352001.0 36 580665.0 37 1069838.0 38 618230.0 39 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.5141679965948 25.64683451441217 14.883875551723113 23.955121937269922 2 12.323037782710948 22.99357649954942 43.50553333186804 21.177852385871596 3 15.44358370487757 31.49954227801583 31.39733784937705 21.659536167729556 4 10.228822528054017 19.684380635667587 42.587046852488236 27.499749983790156 5 11.167921876253516 39.21185549392763 37.296154366052306 12.324068263766552 6 25.2082552605059 42.95685104858447 20.40915290112216 11.42574078978747 7 23.178892917233096 32.91155690313987 28.017997870191586 15.891552309435447 8 22.314358843045866 39.258836561930934 21.87705027029225 16.549754324730944 9 25.050442830908814 15.791133710866967 23.834402450268755 35.32402100795546 10-11 22.10319130580994 28.442228671968845 31.359739257820014 18.09484076440121 12-13 22.42171470457975 25.823748462812425 30.918282064441545 20.836254768166288 14-15 20.236755206606166 27.587697619974556 27.75666522242868 24.418881950990603 16-17 19.311059728048303 29.73658456976881 29.508726389952756 21.443629312230133 18-19 19.105277155332047 30.5145333598552 30.61699879442733 19.76319069038542 20-21 19.80353671281984 29.23105728832968 30.000269248225987 20.96513675062449 22-23 19.903295927977748 29.88980878980066 29.405175720184058 20.801719562037537 24-25 19.837069106678346 29.49581346482904 29.998840584169745 20.668276844322875 26-27 20.14509731949654 29.25883381450006 29.387770745575814 21.20829812042759 28-29 19.84456959297361 30.30120908938052 29.065098726181148 20.78912259146472 30-31 21.04885096945846 29.626330168659287 28.759309669528026 20.565509192354227 32-33 20.823960014989986 29.70507153760519 29.089344803674084 20.381623643730748 34-35 21.13546372786807 28.8252480105853 29.63921561947423 20.4000726420724 36-37 21.18034301125018 29.459451217166826 29.40573894351392 19.954466828069073 38-39 21.67000319663978 28.728670122297917 30.114613341898 19.486713339164304 40-41 20.591371482343888 28.78779011862323 30.44550681036082 20.175331588672066 42-43 21.233331190230086 28.847309938339073 29.51472174551913 20.404637125911712 44-45 20.612998535069856 29.519331473139466 29.51189967261614 20.35577031917454 46-47 21.533415353303127 28.799793063506314 28.795438385565657 20.871353197624902 48-49 21.415068770340458 27.928191409349985 29.746594520874247 20.910145299435307 50-51 21.24200909729312 28.726381710462544 28.55012028252381 21.481488909720518 52-53 20.21221705583109 28.694497775130472 29.384982103235263 21.708303065803168 54-55 20.762788466504972 29.34157269537256 28.554488697618858 21.341150140503615 56-57 21.3435816168301 28.19735015786738 29.256539709717444 21.202528515585076 58-59 20.658495990399377 28.108525717601474 30.30142888385071 20.931549408148438 60-61 20.842354064714087 29.966613219978 28.377444251880068 20.81358846342785 62-63 21.28290460590302 28.970078829286734 27.74355699984944 22.003459564960806 64-65 20.037398029102985 29.033668116943844 28.460183132752565 22.468750721200607 66-67 20.787417042958385 27.816431916498267 28.996973639202295 22.39917740134105 68-69 19.89677483524954 28.70803412373529 28.478278520007926 22.916912521007237 70-71 19.942682419888776 28.435393811778432 29.162551195544932 22.45937257278786 72-73 20.57571589806372 28.67973033416452 29.11422279026882 21.630330977502936 74-75 20.852767413469262 29.348598802421535 27.96060950696907 21.838024277140136 76-77 20.131956075452457 29.32275113086368 29.004881176889498 21.54041161679437 78-79 20.175295794480792 28.886438960726736 28.553129668744525 22.385135576047947 80-81 20.131519820771686 29.228692081431397 29.196312180708695 21.44347591708822 82-83 20.868281632403537 28.366133389566578 29.210332721773874 21.555252256256004 84-85 21.53273997392347 28.37253307063563 28.224607632859172 21.870119322581722 86-87 20.763395523018534 29.172362453865325 28.26786389125741 21.796378131858727 88-89 21.153596771658822 28.07753469730455 28.50884013359108 22.26002839744555 90-91 20.902594358122446 27.80764464504356 29.053659667722915 22.23610132911108 92-93 20.459271718503544 28.69847919578407 28.504865348121655 22.337383737590734 94-95 19.66950389041582 28.1616952401598 29.020266924054088 23.14853394537029 96-97 20.327576716163417 28.173240597730587 28.228534125310656 23.270648560795337 98-99 19.507528510090214 28.94987147518356 28.630510109996145 22.912089904730088 100-101 18.678744394372426 28.50250325742855 28.409983357407743 24.408768990791284 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 87.0 1 56.0 2 43.0 3 59.5 4 45.5 5 43.5 6 68.5 7 126.5 8 200.0 9 245.0 10 216.5 11 214.5 12 284.0 13 327.0 14 356.0 15 400.5 16 466.5 17 566.0 18 665.0 19 715.0 20 814.5 21 1005.0 22 1158.0 23 1327.0 24 1571.5 25 1886.5 26 2476.0 27 3650.5 28 6320.5 29 13188.0 30 25351.5 31 44300.0 32 73293.0 33 114605.5 34 168182.5 35 212900.5 36 250535.0 37 289019.5 38 285743.0 39 235645.0 40 185164.5 41 147235.5 42 123116.5 43 109681.0 44 98611.5 45 95020.0 46 93347.0 47 91905.5 48 93046.5 49 90496.0 50 86928.0 51 85408.0 52 80448.5 53 75564.5 54 72556.5 55 66253.5 56 57731.5 57 48181.0 58 40309.0 59 36970.5 60 31108.5 61 23140.0 62 17179.0 63 13497.5 64 10344.5 65 6740.5 66 4722.0 67 3739.0 68 3037.0 69 2445.5 70 1893.5 71 1408.0 72 1033.0 73 807.0 74 632.0 75 484.5 76 386.0 77 277.0 78 183.5 79 145.5 80 114.5 81 85.0 82 62.5 83 49.5 84 44.5 85 33.0 86 24.0 87 17.5 88 13.0 89 9.0 90 8.0 91 4.5 92 2.0 93 1.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0817635429110239 2 1.0989723509546223E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 3.5716601406025227E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.2363438948239503E-4 40-41 0.002445213480874035 42-43 0.0050003241968435315 44-45 0.0 46-47 0.0 48-49 1.2363438948239503E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.002170470393135379 68-69 0.00671746849521013 70-71 0.0011813952772762191 72-73 0.0 74-75 6.86857719346639E-5 76-77 0.0018682529966228582 78-79 0.00553607321793391 80-81 0.004409626558205422 82-83 0.004437100866979287 84-85 0.002541373561582564 86-87 3.4342885967331946E-4 88-89 0.0 90-91 1.3737154386932779E-5 92-93 0.0012363438948239502 94-95 0.0031458083546076067 96-97 0.002678745105451892 98-99 0.0 100-101 1.7858300703012614E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3639764.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.75996612261747 #Duplication Level Percentage of deduplicated Percentage of total 1 76.1151819087168 27.979915083812955 2 13.056008306297528 9.59876846072219 3 4.424138745291435 4.878935711960175 4 2.0388284907900194 2.9978906500507367 5 1.0846791835245637 1.9936385020135667 6 0.6623237602794583 1.4608199394046502 7 0.43517374343888093 1.1197880446386124 8 0.30291070807454396 0.8907989893598645 9 0.22296481196646764 0.7376561040980812 >10 1.2825884845503221 9.05972248891901 >50 0.16172391372856196 4.196156484087705 >100 0.1776661830204715 13.595729809747143 >500 0.020752840186102305 5.333588800973161 >1k 0.013111000117534919 9.209648181676041 >5k 0.0011238000100744217 2.852101104638328 >10k+ 8.241200073879093E-4 4.094841643897694 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 17800 0.4890426961748069 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 17739 0.48736676333960116 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 16426 0.4512929959195156 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 13984 0.384200733893736 No Hit ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC 13791 0.3788981923003799 No Hit GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC 13062 0.35886942120423193 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 11996 0.3295818080512913 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 11742 0.3226033336227294 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 11198 0.3076573096497465 No Hit CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA 10543 0.2896616374028646 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 10403 0.2858152341745234 No Hit CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA 9093 0.2498238896807595 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 8877 0.24388943898560456 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 8539 0.23460312262003802 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 8042 0.22094839115942683 No Hit TTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 7417 0.20377694817576086 No Hit TAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGG 6922 0.19017716533269738 No Hit CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT 6724 0.18473725219547202 No Hit ATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGA 6488 0.17825331532483973 No Hit CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA 6441 0.17696202281246806 No Hit GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA 6393 0.17564325599132252 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 6262 0.17204412154194612 No Hit CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT 6044 0.16605472222924342 No Hit GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG 5640 0.15495510148460176 No Hit CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC 5432 0.14924044525963773 No Hit TGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATG 5246 0.14413022382769872 No Hit GCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTATACTATATAGGTG 4950 0.13599782843063452 No Hit ATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGT 4823 0.13250859121635358 No Hit CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC 4578 0.12577738556675652 No Hit GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG 4475 0.12294753176304839 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 4474 0.12292005745427452 No Hit GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA 4377 0.12025504950320956 No Hit GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG 4114 0.1130293062956829 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 4062 0.11160064223944191 No Hit TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA 3925 0.10783666193742232 No Hit AATCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTA 3769 0.10355066976869928 No Hit AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA 3757 0.1032209780634129 No Hit CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 3657 0.10047354718602634 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.7474308773865558E-5 0.0 5 0.0 0.0 0.0 2.7474308773865558E-5 0.0 6 0.0 0.0 0.0 2.7474308773865558E-5 0.0 7 0.0 0.0 0.0 2.7474308773865558E-5 0.0 8 0.0 0.0 0.0 2.7474308773865558E-5 0.0 9 0.0 0.0 0.0 5.4948617547731115E-5 0.0 10-11 0.0 0.0 0.0 8.242292632159668E-5 0.0 12-13 0.0 0.0 0.0 1.23634389482395E-4 0.0 14-15 0.0 0.0 0.0 1.3737154386932778E-4 0.0 16-17 0.0 0.0 0.0 1.3737154386932778E-4 0.0 18-19 0.0 0.0 0.0 1.3737154386932778E-4 0.0 20-21 0.0 0.0 0.0 1.6484585264319335E-4 2.7474308773865558E-5 22-23 0.0 0.0 0.0 2.4726877896479E-4 2.7474308773865558E-5 24-25 0.0 0.0 0.0 5.082747123165128E-4 2.7474308773865558E-5 26-27 0.0 0.0 0.0 0.0011401838141154206 5.4948617547731115E-5 28-29 0.0 0.0 0.0 0.0030908597370598754 5.4948617547731115E-5 30-31 0.0 0.0 0.0 0.012432124720174164 5.4948617547731115E-5 32-33 0.0 0.0 0.0 0.045483718175134434 5.4948617547731115E-5 34-35 0.0 0.0 0.0 0.10092687328079514 5.4948617547731115E-5 36-37 0.0 0.0 0.0 0.1850669439007584 8.242292632159668E-5 38-39 0.0 0.0 0.0 0.3049923016986815 8.242292632159668E-5 40-41 0.0 0.0 0.0 0.4332561122094729 8.242292632159668E-5 42-43 0.0 0.0 0.0 0.577097855795046 8.242292632159668E-5 44-45 0.0 0.0 0.0 0.7439493329787316 8.242292632159668E-5 46-47 0.0 0.0 0.0 0.9435913976840257 8.242292632159668E-5 48-49 0.0 0.0 0.0 1.148508529673902 8.242292632159668E-5 50-51 0.0 0.0 0.0 1.3548268514112454 8.242292632159668E-5 52-53 0.0 0.0 0.0 1.5640711870330053 8.242292632159668E-5 54-55 0.0 1.3737154386932779E-5 0.0 1.789772633610311 8.242292632159668E-5 56-57 0.0 2.7474308773865558E-5 0.0 2.057262503832666 8.242292632159668E-5 58-59 0.0 2.7474308773865558E-5 0.0 2.367873301675603 8.242292632159668E-5 60-61 0.0 2.7474308773865558E-5 0.0 2.688306164905197 8.242292632159668E-5 62-63 0.0 2.7474308773865558E-5 0.0 3.0109369728367 8.242292632159668E-5 64-65 0.0 2.7474308773865558E-5 0.0 3.349777622944784 8.242292632159668E-5 66-67 0.0 2.7474308773865558E-5 0.0 3.723606805276386 8.242292632159668E-5 68-69 0.0 2.7474308773865558E-5 0.0 4.127643990104853 8.242292632159668E-5 70-71 0.0 4.121146316079834E-5 0.0 4.5490998866959504 8.242292632159668E-5 72-73 0.0 5.4948617547731115E-5 0.0 5.020174384932649 8.242292632159668E-5 74-75 0.0 5.4948617547731115E-5 0.0 5.453018382510514 8.242292632159668E-5 76-77 0.0 5.4948617547731115E-5 0.0 5.939148801955291 8.242292632159668E-5 78-79 0.0 5.4948617547731115E-5 0.0 6.485228163144644 8.242292632159668E-5 80-81 0.0 5.4948617547731115E-5 0.0 7.091902112334756 9.616008070852946E-5 82-83 0.0 5.4948617547731115E-5 0.0 7.740968919962942 1.0989723509546223E-4 84-85 0.0 5.4948617547731115E-5 0.0 8.35953924485214 1.0989723509546223E-4 86-87 0.0 8.242292632159668E-5 0.0 9.029046938208083 1.0989723509546223E-4 88-89 0.0 8.242292632159668E-5 0.0 9.723185349379794 1.0989723509546223E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCTGGG 3860 0.0 51.55809 3 TACCTGG 4030 0.0 50.56178 2 CCTGGGG 3285 0.0 46.123837 4 CTGGGGG 2735 0.0 44.631954 5 GTACCTG 4585 0.0 44.57503 1 GTACACG 325 0.0 35.098648 1 TGGGGGG 5635 0.0 33.210274 6 TATTCGC 710 0.0 28.09707 9 GTACCCG 390 0.0 28.030172 1 GTTATAA 6380 0.0 26.297628 1 ACCGTGT 590 0.0 25.76132 8 GGGGGGA 5785 0.0 25.61659 7 CGTTAAC 460 0.0 23.764711 1 CTGTGCG 1795 0.0 23.550259 9 ACTATAC 4810 0.0 23.501848 3 TTATAAT 7040 0.0 22.871634 2 ATAATGA 6335 0.0 22.642818 4 CATGGGG 5740 0.0 22.590242 4 TATAATG 6935 0.0 22.53303 3 GTACTAG 2035 0.0 22.421741 1 >>END_MODULE